This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.
trigram | frequency |
---|---|
the detection of | 314 |
for the detection | 295 |
in this study | 260 |
c for min | 236 |
for detection of | 200 |
the presence of | 189 |
according to the | 182 |
polymerase chain reaction | 180 |
as well as | 165 |
based on the | 162 |
respiratory syncytial virus | 153 |
severe acute respiratory | 142 |
acute respiratory syndrome | 138 |
data not shown | 132 |
c for s | 127 |
in order to | 124 |
sensitivity and specificity | 123 |
mediated isothermal amplification | 122 |
the use of | 122 |
infectious bronchitis virus | 122 |
the sensitivity of | 118 |
specificity of the | 116 |
a and b | 115 |
virol methods doi | 115 |
j virol methods | 115 |
a h n | 114 |
was carried out | 114 |
porcine epidemic diarrhea | 113 |
feline infectious peritonitis | 112 |
sensitivity of the | 109 |
of the assay | 106 |
was used to | 106 |
influenza a h | 99 |
pcr assay for | 98 |
the specificity of | 94 |
of the virus | 93 |
development of a | 90 |
epidemic diarrhea virus | 89 |
for min at | 89 |
found to be | 86 |
analysis of the | 84 |
detection of the | 84 |
to determine the | 83 |
due to the | 82 |
sequence of the | 81 |
primers and probes | 79 |
of influenza a | 79 |
the number of | 79 |
were used to | 79 |
the assay was | 78 |
detection limit of | 78 |
the development of | 77 |
the h y | 74 |
a total of | 70 |
the detection limit | 70 |
pcr assay was | 70 |
influenza a virus | 69 |
the expression of | 69 |
serial dilutions of | 67 |
decaro et al | 67 |
assay for the | 65 |
and specificity of | 64 |
rapid detection of | 64 |
was added to | 64 |
used in this | 64 |
rna was extracted | 62 |
transmissible gastroenteritis virus | 62 |
this study was | 62 |
c t values | 61 |
the present study | 61 |
real time pcr | 61 |
sybr green real | 60 |
used in the | 60 |
of the rt | 59 |
was used as | 59 |
influenza a viruses | 58 |
of a novel | 58 |
the diagnosis of | 58 |
the m gene | 58 |
of porcine epidemic | 57 |
the sensitivity and | 57 |
for h at | 57 |
shown in fig | 56 |
of respiratory viruses | 56 |
in the presence | 55 |
the cells were | 55 |
were obtained from | 55 |
of the viral | 54 |
were carried out | 54 |
the detection and | 54 |
and stored at | 53 |
the s protein | 53 |
were positive for | 53 |
can be used | 53 |
of the samples | 52 |
of the s | 52 |
be used to | 51 |
h n and | 51 |
used for the | 51 |
detection of respiratory | 51 |
was extracted from | 51 |
sensitive and specific | 50 |
of viral rna | 49 |
at room temperature | 49 |
c for h | 49 |
assay for detection | 49 |
the n protein | 48 |
was determined by | 48 |
in the present | 48 |
real time rt | 48 |
porcine circovirus type | 48 |
compared to the | 47 |
of respiratory syncytial | 47 |
of severe acute | 47 |
was detected in | 47 |
one of the | 47 |
the results of | 46 |
pcr for the | 45 |
time reverse transcription | 45 |
shown in table | 45 |
pratelli et al | 45 |
method for the | 45 |
as shown in | 45 |
limit of detection | 45 |
and nested pcr | 44 |
was found to | 44 |
the samples were | 44 |
in clinical samples | 44 |
limit of the | 44 |
in the same | 44 |
carried out in | 44 |
detection of sars | 44 |
sequences of the | 44 |
park et al | 43 |
positive and negative | 43 |
o o f | 43 |
were collected from | 43 |
and l of | 43 |
classical swine fever | 43 |
of feline infectious | 43 |
r o o | 43 |
linked immunosorbent assay | 43 |
p r e | 43 |
h n virus | 43 |
p r o | 43 |
a l p | 42 |
of infectious bronchitis | 42 |
u r n | 42 |
was used for | 42 |
j o u | 42 |
was performed using | 42 |
o u r | 42 |
r n a | 42 |
are shown in | 42 |
for rapid detection | 42 |
and differentiation of | 42 |
l p r | 42 |
vero e cells | 42 |
n a l | 42 |
were positive by | 41 |
agarose gel electrophoresis | 41 |
respiratory syndrome coronavirus | 41 |
to assess the | 41 |
pcr products were | 41 |
could be used | 41 |
added to the | 41 |
primers were designed | 41 |
detection and differentiation | 41 |
for min and | 41 |
evaluation of a | 40 |
of canine coronavirus | 40 |
sybr green i | 40 |
were used as | 40 |
more sensitive than | 40 |
for s and | 40 |
time pcr assay | 40 |
was confirmed by | 39 |
all of the | 39 |
were subjected to | 39 |
followed by cycles | 39 |
to evaluate the | 39 |
respiratory tract infections | 39 |
for the presence | 39 |
the absence of | 39 |
of the h | 39 |
primer and probe | 38 |
volume of l | 38 |
the primers and | 38 |
shown to be | 38 |
avian infectious bronchitis | 38 |
pcr assays for | 38 |
were used for | 38 |
dilutions of the | 38 |
with l of | 38 |
the c t | 38 |
the identification of | 38 |
end of the | 38 |
of the primers | 37 |
be used for | 37 |
h n influenza | 37 |
and h n | 37 |
the sequence of | 37 |
the amount of | 37 |
the spike protein | 37 |
l of the | 37 |
newcastle disease virus | 37 |
pcr and nested | 37 |
used to detect | 37 |
virus in the | 37 |
and evaluation of | 36 |
the m protein | 36 |
kim et al | 36 |
the n gene | 36 |
in cell culture | 36 |
fold serial dilutions | 35 |
by cycles of | 35 |
obtained from the | 35 |
used as a | 35 |
samples were positive | 35 |
were found to | 35 |
able to detect | 35 |
results of the | 35 |
detected by the | 35 |
fold dilutions of | 35 |
was supported by | 35 |
this work was | 35 |
the production of | 35 |
copies per reaction | 35 |
comparison of the | 35 |
to confirm the | 34 |
were detected in | 34 |
of the disease | 34 |
of this study | 34 |
detection and quantitation | 34 |
of canine parvovirus | 34 |
the s gene | 34 |
been shown to | 34 |
g for min | 34 |
of the m | 34 |
and respiratory syndrome | 34 |
as described previously | 34 |
detection of human | 34 |
simultaneous detection of | 33 |
canine distemper virus | 33 |
and quantitation of | 33 |
development and evaluation | 33 |
primers and probe | 33 |
tmev and rtv | 33 |
bovine viral diarrhea | 33 |
the viral rna | 33 |
was evaluated by | 33 |
wang et al | 33 |
swine fever virus | 33 |
expression of the | 33 |
respiratory syndrome virus | 33 |
any of the | 33 |
porcine reproductive and | 32 |
were used in | 32 |
west nile virus | 32 |
of the target | 32 |
reproductive and respiratory | 32 |
of the pcr | 32 |
that of the | 32 |
to that of | 32 |
the sybr green | 32 |
detected in the | 32 |
carried out using | 32 |
of the sars | 32 |
and characterization of | 32 |
and identification of | 32 |
the limit of | 31 |
detection of influenza | 31 |
of canine distemper | 31 |
the standard curve | 31 |
included in the | 31 |
the united states | 31 |
the effect of | 31 |
rapid and sensitive | 31 |
by using a | 31 |
showed that the | 31 |
for the diagnosis | 31 |
characterization of a | 31 |
rapid detection and | 31 |
sequence analysis of | 31 |
added to each | 31 |
of porcine circovirus | 31 |
in the study | 31 |
transcription polymerase chain | 31 |
for influenza a | 31 |
influenza a and | 30 |
in addition to | 30 |
the efficiency of | 30 |
to detect the | 30 |
detection of canine | 30 |
assay was developed | 30 |
recombinant m protein | 30 |
were stored at | 30 |
of the two | 30 |
detection of porcine | 30 |
mesenteric lymph nodes | 30 |
positive by the | 29 |
the percentage of | 29 |
as previously described | 29 |
specific for the | 29 |
performed using the | 29 |
to be positive | 29 |
be due to | 29 |
than that of | 29 |
lower respiratory tract | 29 |
for the rapid | 29 |
seasonal h n | 29 |
dilution series of | 29 |
viral diarrhea virus | 29 |
region of the | 29 |
to develop a | 28 |
it has been | 28 |
the aim of | 28 |
for diagnosis of | 28 |
the end of | 28 |
inoculated with the | 28 |
used to determine | 28 |
in the united | 28 |
in comparison to | 28 |
the vaccinia virus | 28 |
was assessed by | 28 |
of each primer | 28 |
reverse transcription loop | 28 |
has been shown | 28 |
three times with | 28 |
used as the | 28 |
the performance of | 27 |
was able to | 27 |
of the real | 27 |
in the field | 27 |
study was to | 27 |
each of the | 27 |
a number of | 27 |
liu et al | 27 |
was performed in | 27 |
and can be | 27 |
l of each | 27 |
n and h | 27 |
the need for | 27 |
a variety of | 27 |
to be a | 27 |
were incubated at | 27 |
bovine respiratory syncytial | 27 |
equine arteritis virus | 27 |
the course of | 27 |
detection of antibodies | 27 |
have been developed | 26 |
the other hand | 26 |
the polymerase chain | 26 |
pcr was performed | 26 |
cells were washed | 26 |
kindly provided by | 26 |
were infected with | 26 |
f and b | 26 |
were detected by | 26 |
a panel of | 26 |
tsv and yhv | 26 |
when compared to | 26 |
of sars coronavirus | 26 |
on the other | 26 |
detection and quantification | 26 |
sequencing of the | 26 |
detection of bovine | 26 |
at the end | 25 |
of newcastle disease | 25 |
of a new | 25 |
by conventional rt | 25 |
h y rt | 25 |
the concentration of | 25 |
was developed for | 25 |
in terms of | 25 |
the influenza a | 25 |
as described above | 25 |
btov and ptov | 25 |
provided by dr | 25 |
in l of | 25 |
is based on | 25 |
viral transport media | 25 |
there was no | 25 |
work was supported | 25 |
at h post | 25 |
the case of | 25 |
in the case | 25 |
samples collected from | 25 |
were negative for | 25 |
there is a | 25 |
for detection and | 24 |
closely related to | 24 |
compared with the | 24 |
nucleic acid amplification | 24 |
ranging from to | 24 |
presence of the | 24 |
of influenza viruses | 24 |
well as the | 24 |
by reverse transcription | 24 |
corresponding to the | 24 |
the addition of | 24 |
none of the | 24 |
type i and | 24 |
a wide range | 24 |
a rapid and | 24 |
rapid diagnosis of | 24 |
multisystemic wasting syndrome | 24 |
as a result | 24 |
in patients with | 24 |
identification of a | 24 |
the reaction was | 24 |
this study were | 24 |
hepatitis c virus | 24 |
porcine epidemic diarrhoea | 24 |
viral rna was | 24 |
derived from the | 24 |
sephadex tm g | 24 |
b and c | 24 |
world health organization | 24 |
the basis of | 24 |
between the two | 24 |
in vitro transcribed | 24 |
li et al | 23 |
to compare the | 23 |
and probes were | 23 |
of the genome | 23 |
a member of | 23 |
use of the | 23 |
use of a | 23 |
a agarose gel | 23 |
suggest that the | 23 |
reverse transcription polymerase | 23 |
of the n | 23 |
such as the | 23 |
incubated for h | 23 |
it is possible | 23 |
of a real | 23 |
phylogenetic analysis of | 23 |
of the three | 23 |
canine parvovirus type | 23 |
in clinical specimens | 23 |
have been described | 23 |
the supernatant was | 23 |
the level of | 23 |
virus infection in | 23 |
a range of | 23 |
h and h | 23 |
washed three times | 23 |
to detect and | 23 |
wide range of | 23 |
of influenza virus | 23 |
samples were collected | 23 |
and quantification of | 23 |
time pcr for | 23 |
developed for the | 23 |
reproducibility of the | 23 |
respiratory viruses in | 23 |
from to copies | 23 |
in vitro transcription | 23 |
out in a | 23 |
sybr green rt | 22 |
of clinical samples | 22 |
and incubated at | 22 |
high sensitivity and | 22 |
indicated that the | 22 |
is the first | 22 |
zhang et al | 22 |
of transmissible gastroenteritis | 22 |
house sephadex tm | 22 |
results showed that | 22 |
of the ibv | 22 |
appears to be | 22 |
evaluation of the | 22 |
were designed to | 22 |
an internal control | 22 |
the lack of | 22 |
sensitive detection of | 22 |
final volume of | 22 |
of reverse transcription | 22 |
value of the | 22 |
tool for the | 22 |
a novel coronavirus | 22 |
casais et al | 22 |
infectious peritonitis virus | 22 |
and sensitive detection | 22 |
pcr for detection | 22 |
of primers and | 22 |
of nucleic acid | 22 |
of h n | 22 |
the in vitro | 22 |
provided by the | 22 |
methods for the | 22 |
was detected by | 22 |
the plates were | 22 |
influenza virus a | 22 |
to be more | 22 |
on the basis | 22 |
performance of the | 22 |
the development and | 22 |
were negative by | 22 |
porcine respiratory coronavirus | 21 |
demonstrated that the | 21 |
was determined using | 21 |
detection of a | 21 |
a set of | 21 |
has been reported | 21 |
be detected by | 21 |
c t value | 21 |
was assessed using | 21 |
have been reported | 21 |
serum samples were | 21 |
of the test | 21 |
the role of | 21 |
determined to be | 21 |
the viral load | 21 |
with the following | 21 |
was performed on | 21 |
related to the | 21 |
h of storage | 21 |
member of the | 21 |
the primers were | 21 |
pdm h n | 21 |
was used in | 21 |
and validation of | 21 |
the pcr products | 21 |
l of template | 21 |
of the duplex | 21 |
of this assay | 21 |
of in vitro | 21 |
dilution of the | 21 |
has not been | 21 |
indicating that the | 21 |
the detection rate | 21 |
aim of this | 21 |
as determined by | 21 |
of antibodies against | 21 |
of the standard | 20 |
samples were tested | 20 |
molecular characterization of | 20 |
regions of the | 20 |
the nucleocapsid protein | 20 |
for at least | 20 |
time pcr assays | 20 |
to each well | 20 |
of sybr green | 20 |
with severe acute | 20 |
pcr was carried | 20 |
the genome of | 20 |
cloned into the | 20 |
supported by the | 20 |
that had been | 20 |
were added to | 20 |
fragment of the | 20 |
there is no | 20 |
be used as | 20 |
swine influenza virus | 20 |
a reverse transcription | 20 |
sephadex tm columns | 20 |
in ml of | 20 |
assay to detect | 20 |
as described by | 20 |
present in the | 20 |
to amplify the | 20 |
of the different | 20 |
cells transfected with | 20 |
from patients with | 20 |
a concentration of | 20 |
genbank accession no | 20 |
of total rna | 20 |
parida et al | 20 |
of feline coronavirus | 20 |
listed in table | 20 |
nucleotide sequence of | 20 |
postweaning multisystemic wasting | 20 |
in our study | 20 |
the in silico | 20 |
reverse transcriptase pcr | 20 |
performed in a | 20 |
coefficient of variation | 20 |
isothermal amplification assay | 20 |
depending on the | 20 |
because of the | 20 |
viral load in | 20 |
of the recombinant | 20 |
diagnosis of sars | 19 |
the gold standard | 19 |
gag and env | 19 |
total rna was | 19 |
chen et al | 19 |
pedv specific siga | 19 |
in the us | 19 |
infections in children | 19 |
rna in the | 19 |
the fact that | 19 |
clinical samples were | 19 |
protein of sars | 19 |
in the faeces | 19 |
an important role | 19 |
the results were | 19 |
in which the | 19 |
of the triplex | 19 |
of the rna | 19 |
would like to | 19 |
with the exception | 19 |
a sensitivity of | 19 |
carried out on | 19 |
efficiency of the | 19 |
a standard curve | 19 |
t rna polymerase | 19 |
of the multiplex | 19 |
in accordance with | 19 |
assay was determined | 19 |
at c for | 19 |
developed in this | 19 |
i and ii | 19 |
of classical swine | 19 |
described for the | 19 |
final concentration of | 19 |
control and prevention | 19 |
incubated for min | 19 |
considered to be | 19 |
specific detection of | 19 |
universal influenza a | 19 |
of these viruses | 19 |
were tested by | 19 |
h n viruses | 19 |
be useful for | 19 |
for the development | 19 |
was designed to | 19 |
a result of | 19 |
were tested for | 19 |
this is the | 19 |
were included in | 19 |
consistent with the | 19 |
assays for the | 19 |
were performed using | 19 |
tested positive for | 19 |
results suggest that | 19 |
a final volume | 19 |
conserved region of | 19 |
the european standard | 18 |
associated with the | 18 |
of the patients | 18 |
coiras et al | 18 |
s protein of | 18 |
diagnosis of fip | 18 |
higher than that | 18 |
amplification of the | 18 |
time reverse transcriptase | 18 |
m of each | 18 |
sensitive than the | 18 |
transfected with pegfp | 18 |
a final concentration | 18 |
of l of | 18 |
in the absence | 18 |
were washed with | 18 |
yang et al | 18 |
the analysis of | 18 |
the sequences of | 18 |
was mixed with | 18 |
copy number of | 18 |
could not be | 18 |
and vaccine strains | 18 |
total volume of | 18 |
the rapid detection | 18 |
detection limits of | 18 |
of the viruses | 18 |
of denaturation at | 18 |
pcr detection of | 18 |
pcr product was | 18 |
each sample was | 18 |
method for detection | 18 |
the surface of | 18 |
and incubated for | 18 |
was shown to | 18 |
by flow cytometry | 18 |
times with pbs | 18 |
the duplex real | 18 |
acute respiratory tract | 18 |
viral rna in | 18 |
strain of pedv | 18 |
basic kit assay | 18 |
false positive results | 18 |
were performed in | 18 |
multiplex reverse transcription | 18 |
viruses in the | 18 |
the nucleotide sequence | 18 |
values of the | 18 |
could be detected | 18 |
the sars coronavirus | 18 |
rna extracted from | 18 |
forward and reverse | 18 |
diagnosis of respiratory | 18 |
rapid identification of | 18 |
carried out with | 18 |
the target sequence | 18 |
serum samples from | 18 |
of the method | 17 |
in this paper | 17 |
thermo fisher scientific | 17 |
cell culture supernatant | 17 |
followed by a | 17 |
specificity and sensitivity | 17 |
was calculated using | 17 |
the primer pair | 17 |
by agarose gel | 17 |
based on a | 17 |
the reaction mixture | 17 |
specific and sensitive | 17 |
human respiratory syncytial | 17 |
performed according to | 17 |
were confirmed by | 17 |
faecal samples of | 17 |
for disease control | 17 |
cells in the | 17 |
involved in the | 17 |
at the same | 17 |
transcriptase polymerase chain | 17 |
in silico sensitivity | 17 |
amino acid residues | 17 |
of avian infectious | 17 |
on the surface | 17 |
was cloned into | 17 |
the virus neutralisation | 17 |
to determine whether | 17 |
the current study | 17 |
in vitro and | 17 |
time pcr system | 17 |
of the cap | 17 |
pandemic influenza a | 17 |
results indicated that | 17 |
were incubated for | 17 |
human immunodeficiency virus | 17 |
for the identification | 17 |
the soiv osel | 17 |
the exception of | 17 |
the ability of | 17 |
rna mini kit | 17 |
with pbs and | 17 |
a mixture of | 17 |
of the study | 17 |
of sars cov | 17 |
the rna was | 17 |
chimaeric s gene | 17 |
and in the | 17 |
observed in the | 17 |
the multiplex method | 17 |
is the most | 17 |
was obtained from | 17 |
submitted to the | 17 |
validation of a | 17 |
of bovine viral | 17 |
ml of the | 17 |
of the other | 17 |
avian influenza virus | 17 |
fetal bovine serum | 17 |
in escherichia coli | 17 |
l of rna | 17 |
samples were negative | 17 |
was observed in | 17 |
rt real time | 17 |
the ee index | 17 |
h y mutation | 17 |
syncytial virus in | 17 |
samples positive for | 17 |
by polymerase chain | 17 |
e and oc | 16 |
with ml of | 16 |
amplification assay for | 16 |
in combination with | 16 |
sequences in the | 16 |
with respect to | 16 |
the virus in | 16 |
the amino acid | 16 |
important role in | 16 |
of samples were | 16 |
for the first | 16 |
assay for rapid | 16 |
the results showed | 16 |
early diagnosis of | 16 |
weeks of age | 16 |
nested pcr assay | 16 |
was tested in | 16 |
to a final | 16 |
is important to | 16 |
the reproducibility of | 16 |
in a total | 16 |
detection of viruses | 16 |
the majority of | 16 |
freymuth et al | 16 |
n protein of | 16 |
min followed by | 16 |
nucleic acid extraction | 16 |
s gene of | 16 |
has been described | 16 |
was isolated from | 16 |
ube d mrna | 16 |
false negative results | 16 |
the universal influenza | 16 |
was performed with | 16 |
was not detected | 16 |
were mixed with | 16 |
significantly higher than | 16 |
sensitivity of rt | 16 |
of the genus | 16 |
a simple and | 16 |
tan et al | 16 |
a positive result | 16 |
assays have been | 16 |
seasonal influenza a | 16 |
influenza viruses a | 16 |
of each dilution | 16 |
the ftdrp assay | 16 |
that the assay | 16 |
quantitative detection of | 16 |
were analyzed by | 16 |
can be detected | 16 |
assays were performed | 16 |
for h n | 16 |
were designed using | 16 |
defined as the | 16 |
disease control and | 16 |
negative by the | 16 |
in the rt | 16 |
agarose gel and | 16 |
in the m | 16 |
of a single | 16 |
sample preparation method | 16 |
time pcr was | 16 |
of the spike | 16 |
to be the | 16 |
chain reaction assay | 16 |
was compared to | 16 |
c until use | 16 |
were compared with | 16 |
s gene sequence | 16 |
probes were designed | 16 |
likely to be | 16 |
a nested pcr | 16 |
using the same | 16 |
detection of h | 16 |
the vero e | 16 |
nested pcr and | 16 |
has also been | 16 |
we found that | 16 |
time quantitative pcr | 16 |
t values of | 16 |
by the in | 16 |
with acute respiratory | 16 |
is one of | 16 |
antigen capture elisa | 16 |
vero e cell | 16 |
of the ns | 16 |
in the first | 15 |
and cycles of | 15 |
assays for detection | 15 |
carried out at | 15 |
developed to detect | 15 |
in silico validation | 15 |
mutations in the | 15 |
and sybr green | 15 |
qiaamp viral rna | 15 |
with a high | 15 |
it should be | 15 |
of viruses in | 15 |
vaccinia virus genome | 15 |
the lamp assay | 15 |
samples that were | 15 |
was determined to | 15 |
marra et al | 15 |
fold dilution series | 15 |
by use of | 15 |
tested using the | 15 |
indicate that the | 15 |
detection of feline | 15 |
by using the | 15 |
of a rapid | 15 |
tested by the | 15 |
primers for the | 15 |
the world health | 15 |
the membrane was | 15 |
have been shown | 15 |
were incubated with | 15 |
viral rna mini | 15 |
the viral genome | 15 |
of the sample | 15 |
the emergence of | 15 |
assay was performed | 15 |
like to thank | 15 |
is able to | 15 |
the nested pcr | 15 |
found in the | 15 |
assay for rsv | 15 |
a total volume | 15 |
reverse transcription and | 15 |
assay can be | 15 |
copies ng of | 15 |
tract infections in | 15 |
virus neutralisation test | 15 |
similar to that | 15 |
a positive control | 15 |
monoclonal antibodies against | 15 |
and used as | 15 |
appeared to be | 15 |
the h n | 15 |
gel electrophoresis and | 15 |
due to its | 15 |
rsv a and | 15 |
the most common | 15 |
he et al | 15 |
be noted that | 15 |
isolated from a | 15 |
it is important | 15 |
for each sample | 15 |
in the clinical | 15 |
containing l of | 15 |
hek t cells | 15 |
were determined by | 15 |
of the fcov | 15 |
lamp assay was | 15 |
m protein was | 15 |
have shown that | 15 |
open reading frame | 15 |
of positive samples | 15 |
higher than the | 15 |
with the same | 15 |
for the n | 15 |
of the plasmid | 15 |
of the same | 15 |
are listed in | 15 |
is shown in | 15 |
were inoculated with | 15 |
study was supported | 15 |
on the same | 15 |
it can be | 15 |
the ministry of | 15 |
assay and the | 15 |
and conventional rt | 15 |
rabies and rabies | 15 |
the conventional rt | 15 |
the method described | 15 |
for the h | 15 |
the replication of | 15 |
real time qt | 15 |
identification of the | 15 |
at postinoculation day | 15 |
were coated with | 15 |
were analyzed using | 15 |
the capsid protein | 15 |
methods such as | 15 |
the importance of | 15 |
in a l | 15 |
detection rate of | 15 |
positive or negative | 15 |
from clinical samples | 15 |
a comparison of | 15 |
have also been | 15 |
hemagglutinating encephalomyelitis virus | 15 |
pandemic h n | 15 |
laboratory diagnosis of | 15 |
by means of | 15 |
the ic assay | 15 |
days after inoculation | 14 |
reaction was performed | 14 |
variola virus specific | 14 |
and for the | 14 |
of the cell | 14 |
is an enveloped | 14 |
of monoclonal antibodies | 14 |
respiratory disease in | 14 |
detection and identification | 14 |
to the detection | 14 |
subtype h n | 14 |
avian influenza viruses | 14 |
most of the | 14 |
a final extension | 14 |
be more sensitive | 14 |
copies of the | 14 |
the highly conserved | 14 |
results indicate that | 14 |
comparison with the | 14 |
bronchitis virus in | 14 |
of the reaction | 14 |
to investigate the | 14 |
analytical sensitivity of | 14 |
the standard deviation | 14 |
were tested in | 14 |
bovine viral diarrhoea | 14 |
large number of | 14 |
been described for | 14 |
isothermal amplification method | 14 |
the reverse transcription | 14 |
was subjected to | 14 |
respiratory virus infections | 14 |
of tmev and | 14 |
and probe were | 14 |
for min in | 14 |
positive samples were | 14 |
syncytial virus infection | 14 |
the assay is | 14 |
for use in | 14 |
used to evaluate | 14 |
the threshold cycle | 14 |
pcr is a | 14 |
bovine and porcine | 14 |
and the results | 14 |
similar to the | 14 |
there was a | 14 |
in nasal aspirates | 14 |
the generation of | 14 |
thompson et al | 14 |
samples from the | 14 |
with and without | 14 |
centrifuged for min | 14 |
oseltamivir resistance in | 14 |
for min followed | 14 |
been developed for | 14 |
wild type and | 14 |
early detection of | 14 |
for rapid diagnosis | 14 |
to test the | 14 |
of the gene | 14 |
development of an | 14 |
copies rna l | 14 |
standard deviation of | 14 |
the control of | 14 |
component of the | 14 |
of the host | 14 |
were resuspended in | 14 |
the qcm biosensor | 14 |
purified using the | 14 |
isothermal amplification for | 14 |
soiv osel res | 14 |
peptides derived from | 14 |
of serum samples | 14 |
an indirect elisa | 14 |
of public health | 14 |
differences between the | 14 |
was developed to | 14 |
the recombinant m | 14 |
patients with severe | 14 |
a combination of | 14 |
the titer of | 14 |
can be found | 14 |
of the most | 14 |
min at room | 14 |
the faecal samples | 14 |
characterization of the | 14 |
without the need | 14 |
influenza b virus | 14 |
and used for | 14 |
has been developed | 14 |
the dilution series | 14 |
the difference between | 14 |
extracted from the | 14 |
on a agarose | 14 |
for rna extraction | 14 |
in vitro transcripts | 14 |
to improve the | 14 |
for quantitation of | 14 |
the coefficient of | 14 |
approved by the | 14 |
those of the | 14 |
as a diagnostic | 14 |
and used to | 14 |
have been used | 14 |
the faeces of | 14 |
epidemic diarrhoea virus | 14 |
assay was evaluated | 14 |
mm of each | 14 |
each well and | 14 |
type and vaccine | 14 |
in the samples | 14 |
of the sequence | 14 |
in stool samples | 14 |
of h y | 14 |
primers used for | 14 |
a ct value | 14 |
the mixture was | 14 |
genbank accession number | 14 |
belonging to the | 14 |
infected vero cells | 14 |
blood samples from | 13 |
coronavirus associated with | 13 |
of cell culture | 13 |
all samples were | 13 |
pcr assay and | 13 |
a negative control | 13 |
and h of | 13 |
were purified using | 13 |
all rights reserved | 13 |
for this study | 13 |
at postchallenge day | 13 |
in the s | 13 |
of infectious virus | 13 |
of detection of | 13 |
for simultaneous detection | 13 |
a l reaction | 13 |
the size of | 13 |
was extracted using | 13 |
lymph node cells | 13 |
the clinical samples | 13 |
to detect all | 13 |
open reading frames | 13 |
amplification and detection | 13 |
of the product | 13 |
assay based on | 13 |
copies l of | 13 |
were washed three | 13 |
highly purified sars | 13 |
distilled water and | 13 |
of hepatitis c | 13 |
the first report | 13 |
determined by testing | 13 |
contained l of | 13 |
centers for disease | 13 |
positive for the | 13 |
of porcine parvovirus | 13 |
a bp fragment | 13 |
in a final | 13 |
useful for the | 13 |
products were detected | 13 |
poon et al | 13 |
in of the | 13 |
clinical diagnosis of | 13 |
the rt step | 13 |
the negative control | 13 |
mab f g | 13 |
l reaction volume | 13 |
carried out to | 13 |
pcr using the | 13 |
and negative by | 13 |
and probe sequences | 13 |
and influenza a | 13 |
caused by the | 13 |
was resuspended in | 13 |
vero cells were | 13 |
genetic characterization of | 13 |
was performed by | 13 |
yellow head virus | 13 |
rsv lna assay | 13 |
the same as | 13 |
were submitted to | 13 |
detection of viral | 13 |
infection in the | 13 |
pcr master mix | 13 |
western blot analysis | 13 |
japanese encephalitis virus | 13 |
the detection limits | 13 |
was based on | 13 |
the ibv cdna | 13 |
step step step | 13 |
of respiratory virus | 13 |
using the primer | 13 |
amino acid sequences | 13 |
the taqman probe | 13 |
in a single | 13 |
was to develop | 13 |
performed on a | 13 |
results were obtained | 13 |
phosphate buffered saline | 13 |
as a template | 13 |
l of extracted | 13 |
the possibility of | 13 |
a previous study | 13 |
the ability to | 13 |
infected with the | 13 |
in the diagnosis | 13 |
and resuspended in | 13 |
the multiplex assay | 13 |
fip and bip | 13 |
the hcov e | 13 |
is known to | 13 |
diagnosis of feline | 13 |
a novel real | 13 |
virus rna in | 13 |
that tested positive | 13 |
is difficult to | 13 |
of enteric viruses | 13 |
belongs to the | 13 |
the respiratory tract | 13 |
h n isolates | 13 |
as per the | 13 |
of the detection | 13 |
by the real | 13 |
c and min | 13 |
were purchased from | 13 |
melting curve analysis | 13 |
development and validation | 13 |
and specificity were | 13 |
three of the | 13 |
and the viral | 13 |
in the positive | 13 |
reverse transcription pcr | 13 |
performed using a | 13 |
soiv osel sen | 13 |
as negative controls | 13 |
to detect a | 13 |
for the study | 13 |
the duplex assay | 13 |
minor groove binder | 13 |
were synthesized by | 13 |
methods have been | 13 |
the chimaeric s | 13 |
performed with the | 13 |
n influenza a | 13 |
embryonated chicken eggs | 13 |
detection of all | 13 |
final extension at | 13 |
the study of | 13 |
strains of pedv | 13 |
be explained by | 13 |
orders of magnitude | 13 |
described in this | 13 |
at a concentration | 13 |
due to a | 13 |
of the five | 13 |
quartz crystal microbalance | 13 |
found that the | 13 |
siga positive colostrum | 13 |
and centrifuged at | 13 |
viral diarrhoea virus | 12 |
a commercially available | 12 |
the most sensitive | 12 |
course of the | 12 |
the amplification of | 12 |
confirmed by sequencing | 12 |
the cap gene | 12 |
to those of | 12 |
the analytical sensitivity | 12 |
small interfering rnas | 12 |
the lamp method | 12 |
a dilution series | 12 |
these samples were | 12 |
to demonstrate the | 12 |
the cell culture | 12 |
wu et al | 12 |
developed sample preparation | 12 |
this study is | 12 |
of the in | 12 |
in our laboratory | 12 |
eluted in l | 12 |
not detected by | 12 |
from clinical specimens | 12 |
cells were infected | 12 |
pcr and real | 12 |
that were negative | 12 |
zhou et al | 12 |
the threshold value | 12 |
time polymerase chain | 12 |
of the infected | 12 |
children with acute | 12 |
porcine transmissible gastroenteritis | 12 |
pcr assay using | 12 |
virus replication in | 12 |
to have a | 12 |
ck s gene | 12 |
which is the | 12 |
need to be | 12 |
pcv capsid protein | 12 |
infected with a | 12 |
no significant difference | 12 |
to study the | 12 |
was defined as | 12 |
experimentally infected with | 12 |
were able to | 12 |
and in vivo | 12 |
results demonstrated that | 12 |
drosten et al | 12 |
in the laboratory | 12 |
in viral transport | 12 |
molecular detection of | 12 |
of viruses from | 12 |
of the individual | 12 |
were cloned into | 12 |
gene of the | 12 |
the severe acute | 12 |
osel sen probe | 12 |
were performed on | 12 |
for up to | 12 |
to obtain a | 12 |
the risk of | 12 |
a review of | 12 |
as a positive | 12 |
and molecular characterization | 12 |
suggested that the | 12 |
the determination of | 12 |
used to generate | 12 |
subjected to a | 12 |
m protein is | 12 |
of the corresponding | 12 |
was stored at | 12 |
pcr and the | 12 |
herrewegh et al | 12 |
peripheral blood mononuclear | 12 |
the range of | 12 |
was extracted and | 12 |
of each sample | 12 |
in parallel with | 12 |
to the sars | 12 |
multiplex pcr assay | 12 |
vitro and in | 12 |
hepatitis b virus | 12 |
be positive by | 12 |
the serum samples | 12 |
oc and e | 12 |
the host cells | 12 |
scov m gene | 12 |
determination of the | 12 |
e and hcov | 12 |
the lamp reaction | 12 |
calculated using the | 12 |
and probes used | 12 |
lee et al | 12 |
was calculated by | 12 |
the fta card | 12 |
expressed as the | 12 |
the coronaviridae family | 12 |
that can be | 12 |
bovine leukemia virus | 12 |
and min at | 12 |
specific siga positive | 12 |
the copy number | 12 |
sequence detection system | 12 |
of dengue virus | 12 |
in the detection | 12 |
gene expression in | 12 |
mouth disease virus | 12 |
we have developed | 12 |
osel res probe | 12 |
in the online | 12 |
limits of the | 12 |
reaction was carried | 12 |
coronavirus infectious bronchitis | 12 |
the binding of | 12 |
accordance with the | 12 |
human influenza a | 12 |
phase of the | 12 |
infected cells were | 12 |
plays an important | 12 |
vitro transcribed rna | 12 |
and reverse primers | 12 |
a large number | 12 |
low levels of | 12 |
should be noted | 12 |
the a l | 12 |
antibodies directed against | 12 |
immunodeficiency virus type | 12 |
the epidemiology of | 12 |
the prevalence of | 12 |
ib h and | 12 |
low viral load | 12 |
may not be | 12 |
large amounts of | 12 |
for the sars | 12 |
molecular epidemiology of | 12 |
by centrifugation at | 12 |
a new coronavirus | 12 |
respiratory viruses by | 12 |
more than one | 12 |
of the new | 12 |
size of the | 12 |
all the samples | 12 |
the design of | 12 |
be able to | 12 |
of which were | 12 |
molecular detection mix | 12 |
an in vitro | 12 |
designed using the | 12 |
been used to | 12 |
test for the | 12 |
samples that tested | 12 |
the virus was | 12 |
multiple sequence alignment | 12 |
amino acid sequence | 12 |
in faecal samples | 12 |
the sense and | 12 |
the clinical signs | 12 |
did not detect | 12 |
a series of | 12 |
mixed with l | 12 |
fcovs type i | 12 |
the online version | 12 |
the wild type | 12 |
dynamic range of | 12 |
huang et al | 12 |
is possible that | 12 |
primer or probe | 12 |
members of the | 12 |
human enteric viruses | 12 |
added to a | 12 |
assay was compared | 12 |
in contrast to | 12 |
respiratory tract infection | 12 |
detection of infectious | 12 |
the university of | 12 |
to identify the | 12 |
included in each | 12 |
to generate a | 11 |
cells were used | 11 |
hepatitis a virus | 11 |
samples were used | 11 |
of rna template | 11 |
was applied to | 11 |
neutralizing antibodies in | 11 |
can also be | 11 |
blood mononuclear cells | 11 |
obtained with the | 11 |
on vero cells | 11 |
cavanagh and naqi | 11 |
quantitation of canine | 11 |
number of the | 11 |
the primers used | 11 |
the authors would | 11 |
used as an | 11 |
to the same | 11 |
majority of the | 11 |
are associated with | 11 |
in south korea | 11 |
to be highly | 11 |
in case of | 11 |
known to be | 11 |
has been used | 11 |
per reaction for | 11 |
antibodies against pedv | 11 |
dna was extracted | 11 |
morbidity and mortality | 11 |
for btov and | 11 |
assay was also | 11 |
at days post | 11 |
this assay was | 11 |
against the s | 11 |
the total antibody | 11 |
vaccine strains of | 11 |
samples tested positive | 11 |
were considered positive | 11 |
during the first | 11 |
and that the | 11 |
gel extraction kit | 11 |
be associated with | 11 |
of viral sequences | 11 |
the genbank database | 11 |
to ensure that | 11 |
change in the | 11 |
part of the | 11 |
taqman real time | 11 |
and application of | 11 |
as a negative | 11 |
well as for | 11 |
reaction mixture was | 11 |
terminus of the | 11 |
ct value of | 11 |
lamp and pcr | 11 |
in vero cells | 11 |
positive for h | 11 |
evaluated by testing | 11 |
coronavirus infections in | 11 |
the optical density | 11 |
on the in | 11 |
to copies of | 11 |
for the assay | 11 |
were as follows | 11 |
rota et al | 11 |
the same samples | 11 |
samples were stored | 11 |
of variola virus | 11 |
viral load and | 11 |
explained by the | 11 |
the application of | 11 |
the cap protein | 11 |
n protein was | 11 |
than conventional rt | 11 |
virulent strains of | 11 |
reactions were performed | 11 |
of f and | 11 |
for the rt | 11 |
effect on the | 11 |
notomi et al | 11 |
was the most | 11 |
the efficacy of | 11 |
of the amplified | 11 |
can be applied | 11 |
have to be | 11 |
viruses a and | 11 |
of the amplicons | 11 |
highly sensitive and | 11 |
in cultured cells | 11 |
the membrane protein | 11 |
the study was | 11 |
amount of viral | 11 |
ms ms analysis | 11 |
all reactions were | 11 |
been used for | 11 |
ministry of agriculture | 11 |
in the negative | 11 |
article can be | 11 |
antibodies against sars | 11 |
lower than that | 11 |
rpm for min | 11 |
in the sample | 11 |
previous studies have | 11 |
avian influenza a | 11 |
were consistent with | 11 |
h n pdm | 11 |
diarrhea virus in | 11 |
and the other | 11 |
by virus isolation | 11 |
porcine hemagglutinating encephalomyelitis | 11 |
presence of a | 11 |
high degree of | 11 |
taq dna polymerase | 11 |
the mesenteric lymph | 11 |
performed as described | 11 |
and h y | 11 |
l well of | 11 |
of the four | 11 |
sequence in the | 11 |
in the future | 11 |
of human respiratory | 11 |
the assessment of | 11 |
resulted in the | 11 |
may be due | 11 |
samples obtained from | 11 |
was eluted in | 11 |
the mlpa and | 11 |
virus and porcine | 11 |
of conventional rt | 11 |
revealed that the | 11 |
nested polymerase chain | 11 |
been reported in | 11 |
specific antibodies in | 11 |
designed based on | 11 |
by gel electrophoresis | 11 |
be used in | 11 |
of the influenza | 11 |
the severity of | 11 |
coronavirus in patients | 11 |
ferret lymph node | 11 |
and of the | 11 |
were not detected | 11 |
and detection of | 11 |
of equine arteritis | 11 |
and stained with | 11 |
the standard rna | 11 |
assay is a | 11 |
from stainless steel | 11 |
for h and | 11 |
been reported to | 11 |
respiratory viral infections | 11 |
tested in triplicate | 11 |
the genotype isolates | 11 |
cell culture and | 11 |
of west nile | 11 |
resuspended in l | 11 |
identified in the | 11 |
from the national | 11 |
collected from the | 11 |
were tested using | 11 |
of the coronavirus | 11 |
in the genbank | 11 |
central nervous system | 11 |
reactivity of the | 11 |
assay developed in | 11 |
the formation of | 11 |
of acute respiratory | 11 |
to achieve the | 11 |
to be specific | 11 |
in vitro rna | 11 |
samples from healthy | 11 |
subjected to lc | 11 |
that were positive | 11 |
assay variability was | 11 |
type and mutant | 11 |
cells were incubated | 11 |
is consistent with | 11 |
rna was isolated | 11 |
these results indicate | 11 |
reverse transcriptase polymerase | 11 |
the results obtained | 11 |
resuspended in ml | 11 |
and vero e | 11 |
ng of total | 11 |
of the full | 11 |
immunosorbent assay for | 11 |
the new assay | 11 |
establishment of a | 11 |
is an important | 11 |
ns serotype specific | 11 |
and sensitivity of | 11 |
fetal calf serum | 11 |
in the control | 11 |
was found in | 11 |
bovine serum albumin | 11 |
the h gene | 11 |
of a multiplex | 11 |
to detect sars | 11 |
pcr assays were | 11 |
this article can | 11 |
sets of primers | 11 |
serotype specific rt | 11 |
in the assay | 11 |
and by the | 11 |
from l of | 11 |
it was demonstrated | 11 |
been detected in | 11 |
virus isolation and | 11 |
portion of the | 11 |
with the corresponding | 11 |
egyptian variant ii | 11 |
and found that | 11 |
the existence of | 11 |
buonavoglia et al | 11 |
rna was eluted | 11 |
of a nested | 10 |
of infected cells | 10 |
cells were harvested | 10 |
with a single | 10 |
and cell cultures | 10 |
in comparison with | 10 |
by grants from | 10 |
subtyping of influenza | 10 |
as a possible | 10 |
number of mismatches | 10 |
and reproducibility of | 10 |
components of the | 10 |
amplified products were | 10 |
quality of the | 10 |
is similar to | 10 |
ability of the | 10 |
faecal samples from | 10 |
feline coronavirus type | 10 |
the occurrence of | 10 |
intensive care unit | 10 |
pigs inoculated with | 10 |
spike and membrane | 10 |
and clinical samples | 10 |
the causative agent | 10 |
viruses that are | 10 |
located in the | 10 |
influenza a strains | 10 |
of extracted rna | 10 |
conserved regions of | 10 |
ct values for | 10 |
of viral load | 10 |
the time of | 10 |
assay was used | 10 |
were also tested | 10 |
viruses were detected | 10 |
of neutralizing antibodies | 10 |
assay could be | 10 |
in the second | 10 |
may be used | 10 |
cells were seeded | 10 |
rad laboratories srl | 10 |
nested pcr were | 10 |
ward et al | 10 |
eggs inoculated with | 10 |
room temperature for | 10 |
the supernatants were | 10 |
were found in | 10 |
supported by grants | 10 |
and n genes | 10 |
nucleic acid sequence | 10 |
depends on the | 10 |
cap protein was | 10 |
detect and differentiate | 10 |
the amplification reaction | 10 |
standard curve was | 10 |
viral rna and | 10 |
the rapid and | 10 |
with ethidium bromide | 10 |
for the in | 10 |
which is a | 10 |
in the real | 10 |
order to detect | 10 |
cause of severe | 10 |
of the first | 10 |
of the total | 10 |
sybr green chemistry | 10 |
clinical signs and | 10 |
the clart pneumovir | 10 |
the viral copy | 10 |
was determined as | 10 |
was also used | 10 |
and specific detection | 10 |
the results indicated | 10 |
were designed based | 10 |
of these mabs | 10 |
respiratory tract disease | 10 |
tested positive by | 10 |
the culture method | 10 |
detected by rt | 10 |
assay was assessed | 10 |
differences in the | 10 |
to determine if | 10 |
negative and positive | 10 |
amplification was carried | 10 |
recombinase polymerase amplification | 10 |
was higher than | 10 |
the reverse primer | 10 |
by the addition | 10 |
is more sensitive | 10 |
herpes simplex virus | 10 |
appear to be | 10 |
of bovine respiratory | 10 |
the quality of | 10 |
for each of | 10 |
compared to that | 10 |
is a member | 10 |
and development of | 10 |
detection of norovirus | 10 |
pair of primers | 10 |
of tsv and | 10 |
described in the | 10 |
plates were incubated | 10 |
children hospitalized with | 10 |
two of the | 10 |
children and adults | 10 |
the extracted rna | 10 |
present study was | 10 |
application of a | 10 |
rv detection kit | 10 |
copies ml for | 10 |
a dilution of | 10 |
of a virus | 10 |
associated with severe | 10 |
cov s protein | 10 |
were separated by | 10 |
was added and | 10 |
the quantitation of | 10 |
for respiratory viruses | 10 |
was compared with | 10 |
the rate of | 10 |
of pcv infection | 10 |
infected and mock | 10 |
were placed in | 10 |
need for a | 10 |
according to their | 10 |
clinical samples from | 10 |
to increase the | 10 |
in vitro model | 10 |
of virus in | 10 |
of sars in | 10 |
chain reaction for | 10 |
the control group | 10 |
results can be | 10 |
with feline infectious | 10 |
the na gene | 10 |
number of viral | 10 |
is associated with | 10 |
that there is | 10 |
the establishment of | 10 |
cycles of denaturation | 10 |
authors would like | 10 |
strain of ibv | 10 |
determined by the | 10 |
of the p | 10 |
institute of public | 10 |
pcr assay to | 10 |
to measure the | 10 |
derived from a | 10 |
were taken from | 10 |
the developed rt | 10 |
on the sequence | 10 |
proved to be | 10 |
of the pandemic | 10 |
disease severity in | 10 |
t easy vector | 10 |
have developed a | 10 |
the dpo assay | 10 |
gene encoding for | 10 |
in young children | 10 |
had to be | 10 |
were prepared in | 10 |
concentration of the | 10 |
used as template | 10 |
to the s | 10 |
in hospitalized children | 10 |
and extension at | 10 |
coefficients of variation | 10 |
standard for the | 10 |
the result of | 10 |
capsid protein of | 10 |
with that of | 10 |
associated with a | 10 |
johnson et al | 10 |
of avian influenza | 10 |
the two assays | 10 |
to obtain the | 10 |
reaction mixture contained | 10 |
in north america | 10 |
the acute phase | 10 |
the sample was | 10 |
samples that had | 10 |
of the developed | 10 |
detection and the | 10 |
with the in | 10 |
a duplex real | 10 |
using a commercial | 10 |
difference between the | 10 |
of the following | 10 |
method for rapid | 10 |
pcr assay were | 10 |
the recovery of | 10 |
to be used | 10 |
this method is | 10 |
with g of | 10 |
the gene encoding | 10 |
of rsv rna | 10 |
the difference in | 10 |
the method is | 10 |
in response to | 10 |
acute lower respiratory | 10 |
out of the | 10 |
of viral respiratory | 10 |
circulating in the | 10 |
in the gut | 10 |
was approved by | 10 |
and the corresponding | 10 |
resistant influenza a | 10 |
ssc and lsc | 10 |
virus from the | 10 |
determine the sensitivity | 10 |
of the c | 10 |
of tgev in | 10 |
the results are | 10 |
virus infections in | 10 |
applicability of the | 10 |
a multiplex real | 10 |
in agreement with | 10 |
probes used in | 10 |
inserted into the | 10 |
a pair of | 10 |
the developed assay | 10 |
of the clinical | 10 |
have been established | 10 |
extracted from l | 10 |
the method was | 10 |
studies have shown | 10 |
and ib d | 10 |
well as a | 10 |
off value of | 10 |
protein is a | 10 |
and a final | 10 |
and the rt | 10 |
is characterized by | 10 |
followed by the | 10 |
of human coronaviruses | 10 |
izzo et al | 10 |
increase in the | 10 |
caused by a | 10 |
the spread of | 10 |
the triplex assay | 10 |
intestinal epithelial cells | 10 |
been described previously | 10 |
naturally infected dogs | 10 |
for min to | 10 |
the host cell | 10 |
van der hoek | 10 |
was incubated at | 10 |
kidney cell line | 10 |
was observed for | 10 |
for both the | 10 |
was performed at | 10 |
it is a | 10 |
at the time | 10 |
of the present | 10 |
of the positive | 10 |
nawagitgul et al | 10 |
in influenza a | 10 |
coronavirus type i | 10 |
for rapid and | 10 |
a and ribv | 10 |
and negative controls | 10 |
confirmed by the | 10 |
of cats with | 10 |
detection of pedv | 10 |
applied to the | 9 |
of lassa virus | 9 |
the quantity of | 9 |
of each serotype | 9 |
were performed according | 9 |
were obtained with | 9 |
of the identified | 9 |
in the number | 9 |
nucleotides in length | 9 |
elisa based on | 9 |
large scale rna | 9 |
in respiratory samples | 9 |
a laboratory manual | 9 |
to serve as | 9 |
compared with conventional | 9 |
and public health | 9 |
in cell cultures | 9 |
the onset of | 9 |
increasing number of | 9 |
the pedv m | 9 |
were centrifuged at | 9 |
in relation to | 9 |
on the results | 9 |
did not cross | 9 |
a sensitive and | 9 |
was performed as | 9 |
that could be | 9 |
the highest dilution | 9 |
in the cell | 9 |
pcr assay described | 9 |
of the expected | 9 |
different concentrations of | 9 |
plates were washed | 9 |
were amplified by | 9 |
nested pcr assays | 9 |
was measured by | 9 |
by in vitro | 9 |
infectious bursal disease | 9 |
were observed in | 9 |
who taqman assay | 9 |
as described in | 9 |
the differences in | 9 |
for tmev and | 9 |
because of its | 9 |
transmissible gastroenteritis coronavirus | 9 |
as the target | 9 |
the indirect elisa | 9 |
that the expression | 9 |
using the nuclisens | 9 |
were provided by | 9 |
designed to detect | 9 |
chain reaction and | 9 |
l of cdna | 9 |
ranged from to | 9 |
in hong kong | 9 |
a group of | 9 |
rna and dna | 9 |
heaton et al | 9 |
were cultured in | 9 |
were selected for | 9 |
describes the development | 9 |
per primer or | 9 |
of the sensitivity | 9 |
in the family | 9 |
amplification method for | 9 |
accuracy of the | 9 |
time consuming and | 9 |
for the production | 9 |
of antibodies to | 9 |
loop mediated isothermal | 9 |
nucleotide sequences of | 9 |
h and n | 9 |
the duplex rrt | 9 |
cells were grown | 9 |
load in the | 9 |
length ibv cdna | 9 |
fold serially diluted | 9 |
a maximum of | 9 |
second round of | 9 |
study was approved | 9 |
the tqm probe | 9 |
american type culture | 9 |
fecal samples from | 9 |
of scov m | 9 |
in some cases | 9 |
been shown that | 9 |
the pathogenesis of | 9 |
in a co | 9 |
of hnov gii | 9 |
culture supernatants were | 9 |
amplification products were | 9 |
of the elisa | 9 |
novel h n | 9 |
compared to a | 9 |
other respiratory viruses | 9 |
type a influenza | 9 |
and env genes | 9 |
upper respiratory tract | 9 |
the reaction conditions | 9 |
pcr assay is | 9 |
as it is | 9 |
simultaneous detection and | 9 |
days after the | 9 |
detected down to | 9 |
this assay is | 9 |
patients with a | 9 |
reference strains of | 9 |
variant strain of | 9 |
the discovery of | 9 |
was serially diluted | 9 |
infected vero cell | 9 |
was observed with | 9 |
the commercially available | 9 |
of the human | 9 |
time pcr using | 9 |
and cells were | 9 |
the results suggest | 9 |
and membrane proteins | 9 |
gunson et al | 9 |
the amplified product | 9 |
during the course | 9 |
and days post | 9 |
it is also | 9 |
by rna interference | 9 |
we developed a | 9 |
has been found | 9 |
influenza h n | 9 |
vp and vp | 9 |
was achieved by | 9 |
the apical surface | 9 |
developed in the | 9 |
high prevalence of | 9 |
defined in the | 9 |
of gene expression | 9 |
up to h | 9 |
gold standard for | 9 |
l of mm | 9 |
but it is | 9 |
copy numbers of | 9 |
the two groups | 9 |
relationship between the | 9 |
step of the | 9 |
an abi prism | 9 |
of the manuscript | 9 |
the centers for | 9 |
the negative group | 9 |
series of a | 9 |
influenza virus infection | 9 |
sense rna genome | 9 |
for the analysis | 9 |
immunochromatographic test strip | 9 |
denaturation step at | 9 |
m gene of | 9 |
in a variety | 9 |
samples of the | 9 |
was incubated for | 9 |
a viruses and | 9 |
was incubated with | 9 |
free water and | 9 |
of these two | 9 |
type culture collection | 9 |
detection of nv | 9 |
this study and | 9 |
the test was | 9 |
in the n | 9 |
used to design | 9 |
site of the | 9 |
in western immunoblot | 9 |
of the results | 9 |
and rapid detection | 9 |
the duration of | 9 |
molecular detection methods | 9 |
of sensitivity and | 9 |
the same sample | 9 |
in out of | 9 |
was reverse transcribed | 9 |
the alignment search | 9 |
with a mean | 9 |
and the plates | 9 |
of the na | 9 |
stewart et al | 9 |
coronavirus nucleocapsid protein | 9 |
times lower than | 9 |
it was found | 9 |
to the other | 9 |
negative by both | 9 |
were determined using | 9 |
shin et al | 9 |
as a control | 9 |
pcr assay targeting | 9 |
characterization of porcine | 9 |
acid sequences of | 9 |
isolation and identification | 9 |
the pcr product | 9 |
to the cell | 9 |
of a coronavirus | 9 |
assay and inter | 9 |
in the course | 9 |
to estimate the | 9 |
cells infected with | 9 |
and quantitative detection | 9 |
were positive according | 9 |
resistance to oseltamivir | 9 |
by a grant | 9 |
information on the | 9 |
to quantify the | 9 |
pigs from group | 9 |
were collected at | 9 |
suggesting that the | 9 |
lu et al | 9 |
chain reaction assays | 9 |
ge pfu ratio | 9 |
total rna from | 9 |
we used the | 9 |
the potential of | 9 |
min and min | 9 |
a useful tool | 9 |
onto fta cards | 9 |
were identified by | 9 |
from the same | 9 |
used to assess | 9 |
fluorescence intensity of | 9 |
of the serum | 9 |
lamp products were | 9 |
assay for detecting | 9 |
for the clinical | 9 |
viral culture and | 9 |
available in the | 9 |
the standard curves | 9 |
under isothermal conditions | 9 |
assay has been | 9 |
serial dilution of | 9 |
the first time | 9 |
in a ml | 9 |
vries et al | 9 |
in the current | 9 |
analysis showed that | 9 |
in fecal specimens | 9 |
days of incubation | 9 |
of the protein | 9 |
at and h | 9 |
equal volume of | 9 |
of nucleic acids | 9 |
the positive samples | 9 |
reverse transcription was | 9 |
serve as a | 9 |
suspected to have | 9 |
with respiratory tract | 9 |
were also included | 9 |
subgroups a and | 9 |
science and technology | 9 |
with high sensitivity | 9 |
enteric viruses from | 9 |
total antibody test | 9 |
results in the | 9 |
quantitation of the | 9 |
le et al | 9 |
h post infection | 9 |
viral nucleic acid | 9 |
and phylogenetic analysis | 9 |
causative agent of | 9 |
and the probe | 9 |
agreement with the | 9 |
for use as | 9 |
lymphocyte proliferative responses | 9 |
useful tool for | 9 |
on fta cards | 9 |
body cavity effusions | 9 |
detected with the | 9 |
a high specificity | 9 |
of bluetongue virus | 9 |
then added to | 9 |
it is more | 9 |
primers used in | 9 |
detection of fcov | 9 |
sd bioline norovirus | 9 |
in mammalian cells | 9 |
one hundred and | 9 |
the ct values | 9 |
to the number | 9 |
for each virus | 9 |
plasmid dna was | 9 |
techniques for the | 9 |
the forward primer | 9 |
the virulent pedv | 9 |
well and the | 9 |
and analyzed by | 9 |
by the multiplex | 9 |
direct detection of | 9 |
stainless steel surfaces | 9 |
with the h | 9 |
after h of | 9 |
sensitive than conventional | 9 |
of the family | 9 |
samples were analyzed | 9 |
used to monitor | 9 |
in the gene | 9 |
positive according to | 9 |
scale rna production | 9 |
h and ib | 9 |
human coronavirus e | 9 |
infected vero e | 9 |
protein and the | 9 |
of the world | 9 |
the accuracy of | 9 |
of oseltamivir resistance | 9 |
the reverse transcriptase | 9 |
infected with wild | 9 |
concentrations ranging from | 9 |
the ecogpt gene | 9 |
could be a | 9 |
of porcine reproductive | 9 |
c virus rna | 9 |
gelb and jackwood | 9 |
nf en a | 9 |
analysis of porcine | 9 |
with a final | 9 |
rna extraction kit | 9 |
this study could | 9 |
cdc real time | 9 |
tof ms and | 9 |
of btov and | 9 |
pcr was determined | 9 |
rna extraction and | 9 |
the infectivity of | 9 |
mouse hepatitis virus | 9 |
the molecular assays | 9 |
the virus is | 9 |
viruses associated with | 9 |
peiris et al | 9 |
table comparison of | 9 |
it is difficult | 9 |
proven to be | 9 |
taku et al | 9 |
nested pcr system | 9 |
of rnase inhibitor | 9 |
protein gene of | 9 |
the need to | 9 |
clinical samples by | 9 |
and probes for | 9 |
fouchier et al | 9 |
the structural proteins | 9 |
of up to | 9 |
for rsv a | 9 |
was purchased from | 9 |
in the mesenteric | 9 |
by the polymerase | 9 |
primers defined in | 9 |
in this assay | 9 |
are reported in | 9 |
by viral isolation | 9 |
which can be | 9 |
were designed and | 9 |
the genomic rna | 9 |
by real time | 9 |
was provided by | 9 |
of the ic | 9 |
quantitative reverse transcription | 9 |
carried out by | 9 |
the amplified products | 9 |
used in a | 9 |
protein of porcine | 9 |
directed against the | 9 |
temperature for min | 9 |
were examined for | 9 |
may result in | 9 |
the reaction mix | 9 |
that have been | 9 |
normal human serum | 9 |
the ns gene | 9 |
lamp assay is | 9 |
glass et al | 9 |
with virulent pedv | 8 |
sun et al | 8 |
obtained using the | 8 |
m protein of | 8 |
one step rt | 8 |
was observed when | 8 |
related to this | 8 |
is useful for | 8 |
value for the | 8 |
production of the | 8 |
fecal samples that | 8 |
samples with a | 8 |
each dilution was | 8 |
properties of the | 8 |
of respiratory viral | 8 |
were grown in | 8 |
syncytial virus and | 8 |
high sensitivity of | 8 |
combined with the | 8 |
s protein fragments | 8 |
coronavirus spike protein | 8 |
data were analyzed | 8 |
the type of | 8 |
with the rt | 8 |
of nested primers | 8 |
of the cells | 8 |
extracted using the | 8 |
were identified as | 8 |
filtration on the | 8 |
number of cells | 8 |
will be useful | 8 |
treated with the | 8 |
the variant pedv | 8 |
primer probe sets | 8 |
were selected to | 8 |
the lod of | 8 |
to amplify a | 8 |
summarized in table | 8 |
type ii and | 8 |
changes in the | 8 |
the potential to | 8 |
purified using a | 8 |
virulent strain of | 8 |
the human coronavirus | 8 |
c in a | 8 |
analysis of a | 8 |
samples were obtained | 8 |
ftdrp ev pev | 8 |
the age of | 8 |
and western blotting | 8 |
and amino acid | 8 |
rna or dna | 8 |
titer of the | 8 |
julian et al | 8 |
were harvested and | 8 |
of agricultural sciences | 8 |
methods for detection | 8 |
the cdc real | 8 |
probe for the | 8 |
between groups and | 8 |
scov m protein | 8 |
dilutions of a | 8 |
new england biolabs | 8 |
diagnosis of human | 8 |
was purified using | 8 |
of norovirus in | 8 |
of this paper | 8 |
l of rnase | 8 |
n gene was | 8 |
was included in | 8 |
rna copy number | 8 |
virus by real | 8 |
is a rapid | 8 |
in table s | 8 |
assay was highly | 8 |
yellow fever virus | 8 |
carr et al | 8 |
for clinical diagnosis | 8 |
the endpoint detection | 8 |
as an internal | 8 |
and disease severity | 8 |
probe were designed | 8 |
was selected as | 8 |
viruses in clinical | 8 |
the combination of | 8 |
clinical signs of | 8 |
primers and the | 8 |
of wild type | 8 |
set of four | 8 |
positive rate of | 8 |
s protein and | 8 |
times more sensitive | 8 |
required for the | 8 |
the advantages of | 8 |
of standard rna | 8 |
resulting in the | 8 |
of the lamp | 8 |
the amplification efficiency | 8 |
hoek et al | 8 |
reported in table | 8 |
of the porcine | 8 |
isolation and characterization | 8 |
dual priming oligonucleotide | 8 |
by the rt | 8 |
the recombinant plasmid | 8 |
reaction volume containing | 8 |
three multiplex rt | 8 |
be applied to | 8 |
a prospective study | 8 |
a minimum of | 8 |
ic and cc | 8 |
and western blot | 8 |
in the total | 8 |
of the capsid | 8 |
for influenza virus | 8 |
multiplicity of infection | 8 |
to rsv infection | 8 |
polyacrylamide gel electrophoresis | 8 |
nucleic acid from | 8 |
limits of detection | 8 |
coronavirus as a | 8 |
capable of detecting | 8 |
antigen detection tests | 8 |
number of copies | 8 |
acute phase of | 8 |
is responsible for | 8 |
range of the | 8 |
determined using a | 8 |
detection of tgev | 8 |
were shown to | 8 |
an alternative to | 8 |
a detection limit | 8 |
out of samples | 8 |
celigo image cytometer | 8 |
fcovs type ii | 8 |
selected based on | 8 |
care and use | 8 |
of ube d | 8 |
and vero cells | 8 |
reaction volume of | 8 |
the nucleotide sequences | 8 |
of viral rnas | 8 |
reverse transcription step | 8 |
given in table | 8 |
recovery efficiencies of | 8 |
have been identified | 8 |
the choice of | 8 |
the tubes were | 8 |
was evaluated using | 8 |
of each of | 8 |
is a newly | 8 |
three independent experiments | 8 |
as little as | 8 |
cell line po | 8 |
the most abundant | 8 |
and b primers | 8 |
to be tested | 8 |
used as positive | 8 |
of bovine leukemia | 8 |
washed with pbs | 8 |
in respiratory specimens | 8 |
virus with a | 8 |
be detected in | 8 |
in the orf | 8 |
associated with this | 8 |
for rsv b | 8 |
stranded rna virus | 8 |
sterile distilled water | 8 |
dengue virus serotypes | 8 |
prevention and control | 8 |
of the amplification | 8 |
clinical samples was | 8 |
the yield of | 8 |
were performed with | 8 |
cho et al | 8 |
identity of the | 8 |
for the simultaneous | 8 |
set of primers | 8 |
samples for the | 8 |
cook et al | 8 |
positive samples by | 8 |
of feline coronaviruses | 8 |
the novel h | 8 |
and genotyping of | 8 |
in the human | 8 |
feline enteric coronaviruses | 8 |
obtained by the | 8 |
after the incubation | 8 |
that the viral | 8 |
because it is | 8 |
disease virus by | 8 |
was performed for | 8 |
were derived from | 8 |
influenza c virus | 8 |
of s at | 8 |
flow cytometry and | 8 |
the probe was | 8 |
mononuclear cells from | 8 |
alignment of the | 8 |
a l and | 8 |
products were electrophoresed | 8 |
and comparison of | 8 |
their ability to | 8 |
the introduction of | 8 |
by the soiv | 8 |
the internal control | 8 |
system for the | 8 |
in recent years | 8 |
and newcastle disease | 8 |
rapid and reliable | 8 |
a monoclonal antibody | 8 |
of denv infection | 8 |
viruses in a | 8 |
was demonstrated that | 8 |
supported by a | 8 |
of porcine transmissible | 8 |
the family coronaviridae | 8 |
viral transport medium | 8 |
green i real | 8 |
a specificity of | 8 |
followed by amplification | 8 |
isothermal amplification of | 8 |
were performed by | 8 |
used to amplify | 8 |
amplification for rapid | 8 |
the applicability of | 8 |
north american swine | 8 |
kuypers et al | 8 |
novel coronavirus in | 8 |
after incubation at | 8 |
of primers were | 8 |
ccv and ccv | 8 |
when compared with | 8 |
it was also | 8 |
responsible for the | 8 |
collected from patients | 8 |
antibodies in the | 8 |
in the virus | 8 |
are grateful to | 8 |
is likely to | 8 |
the following formula | 8 |
were calculated using | 8 |
is due to | 8 |
method was developed | 8 |
was digested with | 8 |
dawood et al | 8 |
used as negative | 8 |
house developed sample | 8 |
national center for | 8 |
only a few | 8 |
by the national | 8 |