This is a table of type bigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.
bigram | frequency |
---|---|
time rt | 486 |
pcr assay | 471 |
time pcr | 308 |
reverse transcription | 263 |
viral rna | 216 |
acute respiratory | 193 |
polymerase chain | 186 |
chain reaction | 182 |
respiratory syndrome | 179 |
clinical samples | 177 |
sybr green | 176 |
nested pcr | 173 |
influenza virus | 165 |
infectious bronchitis | 159 |
respiratory syncytial | 156 |
pcr assays | 156 |
real time | 154 |
syncytial virus | 153 |
severe acute | 144 |
porcine epidemic | 138 |
cell culture | 137 |
isothermal amplification | 136 |
conventional rt | 135 |
respiratory viruses | 134 |
nucleic acid | 128 |
bronchitis virus | 122 |
mediated isothermal | 122 |
diarrhea virus | 119 |
virol methods | 115 |
cord uid | 115 |
doc id | 115 |
methods doi | 115 |
epidemic diarrhea | 114 |
infectious peritonitis | 113 |
feline infectious | 113 |
detection limit | 112 |
positive samples | 110 |
respiratory tract | 109 |
influenza viruses | 108 |
reverse transcriptase | 107 |
rapid detection | 106 |
agarose gel | 103 |
lamp assay | 103 |
applied biosystems | 102 |
serum samples | 102 |
transmissible gastroenteritis | 90 |
cell lines | 89 |
viral load | 87 |
pcr products | 85 |
infected cells | 84 |
serial dilutions | 84 |
amino acid | 84 |
gel electrophoresis | 82 |
copy number | 82 |
virus infection | 80 |
monoclonal antibodies | 80 |
canine coronavirus | 79 |
cell line | 79 |
vero cells | 76 |
porcine circovirus | 74 |
time reverse | 74 |
standard curve | 73 |
rna extraction | 72 |
disease virus | 72 |
step rt | 70 |
performed using | 70 |
virus isolation | 69 |
spike protein | 69 |
cell cultures | 69 |
sars coronavirus | 69 |
feline coronavirus | 68 |
avian influenza | 68 |
type i | 67 |
green real | 66 |
canine parvovirus | 65 |
positive results | 65 |
sephadex tm | 64 |
per reaction | 64 |
clinical specimens | 64 |
dna polymerase | 63 |
negative control | 63 |
clinical signs | 63 |
gastroenteritis virus | 63 |
present study | 63 |
total rna | 62 |
fecal samples | 61 |
pcr kit | 61 |
sequence analysis | 61 |
ct values | 60 |
described previously | 60 |
monoclonal antibody | 60 |
dilution series | 59 |
internal control | 58 |
capsid protein | 57 |
gene expression | 57 |
oseltamivir resistance | 56 |
three times | 56 |
tested positive | 56 |
newcastle disease | 56 |
pcr using | 55 |
pcr product | 54 |
ms ms | 53 |
serially diluted | 53 |
room temperature | 52 |
faecal samples | 52 |
multiplex pcr | 52 |
copies ml | 52 |
reaction mixture | 51 |
linked immunosorbent | 50 |
fold dilutions | 50 |
immune response | 50 |
sars cov | 50 |
vaccinia virus | 49 |
classical swine | 49 |
nucleocapsid protein | 49 |
copies per | 49 |
multiplex real | 49 |
specific antibodies | 48 |
bovine viral | 48 |
closely related | 48 |
flow cytometry | 48 |
human coronavirus | 48 |
immunosorbent assay | 48 |
fever virus | 48 |
nucleotide sequence | 48 |
pcr system | 48 |
circovirus type | 48 |
canine distemper | 47 |
transfected cells | 47 |
escherichia coli | 47 |
swine fever | 46 |
rna copies | 46 |
nucleic acids | 46 |
lymph nodes | 46 |
distilled water | 46 |
duplex real | 46 |
pcr amplification | 46 |
positive control | 45 |
respiratory disease | 45 |
vaccine strains | 45 |
neutralizing antibodies | 45 |
high sensitivity | 45 |
variola virus | 45 |
multiplex rt | 45 |
days post | 45 |
public health | 45 |
virus type | 44 |
electron microscopy | 44 |
simultaneous detection | 43 |
blood samples | 43 |
like particles | 43 |
wild type | 43 |
united states | 43 |
syndrome coronavirus | 42 |
molecular detection | 42 |
enteric viruses | 42 |
genbank accession | 42 |
assay variability | 42 |
basic kit | 42 |
specific rt | 41 |
cap protein | 41 |
virus rna | 41 |
plasmid dna | 41 |
viral isolation | 41 |
commercially available | 41 |
sars patients | 41 |
rna viruses | 40 |
previously described | 40 |
fold serial | 40 |
copy numbers | 40 |
green i | 40 |
epithelial cells | 40 |
syndrome virus | 40 |
samples tested | 39 |
avian infectious | 39 |
samples collected | 39 |
human metapneumovirus | 39 |
buffered saline | 39 |
molecular methods | 39 |
negative results | 39 |
tract infections | 39 |
respiratory samples | 38 |
indirect elisa | 38 |
mm tris | 38 |
virus detection | 38 |
nasal aspirates | 38 |
standard deviation | 38 |
negative controls | 38 |
virus infections | 38 |
virus replication | 38 |
sample preparation | 38 |
western blot | 37 |
determined using | 37 |
pcr method | 37 |
protein gene | 37 |
tcid ml | 37 |
lower respiratory | 37 |
postinoculation day | 37 |
atcc vr | 37 |
nested rt | 37 |
rapid diagnosis | 36 |
pcr tests | 36 |
virus isolates | 36 |
bovine respiratory | 36 |
detection rate | 36 |
novel coronavirus | 36 |
swine influenza | 36 |
life technologies | 36 |
respiratory virus | 36 |
porcine parvovirus | 36 |
free water | 35 |
west nile | 35 |
highly conserved | 35 |
viral genome | 35 |
genomic rna | 35 |
reverse primer | 35 |
type ii | 35 |
dengue virus | 35 |
genome copies | 35 |
transport media | 34 |
analytical sensitivity | 34 |
virus strains | 34 |
sequence alignment | 34 |
phylogenetic analysis | 34 |
conventional methods | 34 |
rna virus | 34 |
structural proteins | 34 |
membrane protein | 34 |
standard curves | 34 |
reaction volume | 34 |
quantitative pcr | 34 |
mass spectrometry | 33 |
viral diarrhea | 33 |
pcr results | 33 |
host cells | 33 |
porcine reproductive | 33 |
specific primers | 33 |
transcription loop | 33 |
rsv infection | 33 |
like pestivirus | 33 |
stranded rna | 33 |
infected vero | 33 |
primer probe | 33 |
rna transcripts | 33 |
purified using | 33 |
pcr test | 33 |
nile virus | 33 |
fusion protein | 33 |
distemper virus | 33 |
viral transport | 33 |
mesenteric lymph | 33 |
rna polymerase | 33 |
accession number | 32 |
highly sensitive | 32 |
conventional pcr | 32 |
respiratory pathogens | 32 |
samples containing | 32 |
forward primer | 32 |
diarrhoea virus | 32 |
virus strain | 32 |
respiratory specimens | 32 |
molecular techniques | 32 |
human respiratory | 32 |
viral infections | 32 |
cov infection | 32 |
primer pair | 31 |
mononuclear cells | 31 |
transcription polymerase | 31 |
immune responses | 31 |
pcr reaction | 31 |
surface sampling | 31 |
negative samples | 31 |
fta cards | 31 |
mini kit | 31 |
viral sequences | 31 |
bovine serum | 31 |
stool samples | 30 |
detection methods | 30 |
sensitive detection | 30 |
results showed | 30 |
extraction kit | 30 |
experimentally infected | 30 |
detection limits | 30 |
protein expression | 30 |
false positive | 30 |
detection system | 30 |
fold dilution | 30 |
rna interference | 30 |
tissue culture | 30 |
standard rna | 30 |
goat anti | 30 |
two different | 29 |
stainless steel | 29 |
final concentration | 29 |
specific siga | 29 |
well plates | 29 |
mg ml | 29 |
pcr detection | 29 |
world health | 29 |
molecular assays | 29 |
immunofluorescence assay | 29 |
assay using | 29 |
highly specific | 28 |
calculated using | 28 |
significantly higher | 28 |
time quantitative | 28 |
infectious virus | 28 |
multiplex assay | 28 |
parainfluenza virus | 28 |
primers used | 28 |
western blotting | 28 |
gene sequence | 28 |
recovery efficiencies | 28 |
equine arteritis | 28 |
viral culture | 28 |
arteritis virus | 28 |
pedv infection | 28 |
extracted rna | 28 |
ct value | 28 |
gene sequences | 28 |
amplification assay | 28 |
viral proteins | 28 |
false negative | 28 |
open reading | 28 |
kindly provided | 28 |
sanger sequencing | 27 |
virus genome | 27 |
amplification efficiency | 27 |
viral infection | 27 |
amino acids | 27 |
primer set | 27 |
high specificity | 27 |
host cell | 27 |
infected cell | 27 |
naturally infected | 27 |
viral loads | 27 |
coronavirus infection | 27 |
using primers | 27 |
soiv osel | 27 |
encephalomyelitis virus | 27 |
viral pathogens | 27 |
ethidium bromide | 27 |
cell lysates | 27 |
realtime pcr | 27 |
gold standard | 27 |
target sequence | 27 |
pandemic influenza | 27 |
results obtained | 26 |
virulent pedv | 26 |
assay detected | 26 |
respiratory infections | 26 |
master mix | 26 |
copies reaction | 26 |
capture elisa | 26 |
wasting syndrome | 26 |
virus neutralisation | 26 |
dna vaccine | 26 |
quantitative rt | 26 |
transcriptase pcr | 26 |
housekeeping genes | 26 |
associated coronavirus | 26 |
ee index | 25 |
wide range | 25 |
reaction mix | 25 |
taqman rt | 25 |
seasonal influenza | 25 |
type strains | 25 |
diagnostic methods | 25 |
genomic dna | 25 |
quantitative real | 25 |
threshold cycle | 25 |
sense rna | 25 |
final extension | 25 |
taqman assay | 25 |
peripheral blood | 25 |
pcr buffer | 25 |
time consuming | 25 |
rna genome | 25 |
culture supernatant | 25 |
vitro transcribed | 25 |
final volume | 25 |
assays using | 25 |
viral copy | 25 |
pseudorabies virus | 25 |
enzyme mix | 24 |
taqman probe | 24 |
assessed using | 24 |
clinical diagnosis | 24 |
samples positive | 24 |
health organization | 24 |
using real | 24 |
viral copies | 24 |
economic losses | 24 |
respiratory coronavirus | 24 |
multisystemic wasting | 24 |
human coronaviruses | 24 |
total antibody | 24 |
epidemic diarrhoea | 24 |
dynamic range | 24 |
acid amplification | 24 |
specific primer | 24 |
rna template | 23 |
washed three | 23 |
specific detection | 23 |
diagnostic laboratories | 23 |
lamp method | 23 |
tissue samples | 23 |
elution buffer | 23 |
related viruses | 23 |
multiplex reverse | 23 |
also used | 23 |
genome sequence | 23 |
bovine coronavirus | 23 |
lamp reaction | 23 |
tested using | 23 |
variant pedv | 23 |
nasal swabs | 23 |
parvovirus type | 23 |
vitro transcription | 23 |
transcribed rna | 23 |
virus dna | 23 |
rna isolation | 23 |
ibv cdna | 23 |
antigen detection | 23 |
viruses using | 23 |
infected dogs | 23 |
viral replication | 23 |
cytopathic effect | 23 |
enrichment methods | 23 |
lymph node | 23 |
foster city | 23 |
virus specific | 22 |
peritonitis virus | 22 |
primer sets | 22 |
whole blood | 22 |
porcine respiratory | 22 |
initial denaturation | 22 |
reference strains | 22 |
laboratory diagnosis | 22 |
encephalitis virus | 22 |
green rt | 22 |
previous studies | 22 |
pcr methods | 22 |
allantoic fluid | 22 |
well plate | 22 |
expression vector | 22 |
molecular characterization | 22 |
contact time | 22 |
animal health | 22 |
amplification products | 22 |
structural protein | 22 |
nucleotide sequences | 22 |
oligonucleotide primers | 22 |
positive result | 22 |
infectious diseases | 22 |
swab samples | 22 |
rna extracted | 22 |
taqman probes | 22 |
acid sequence | 22 |
amplification method | 22 |
disease control | 22 |
house sephadex | 22 |
fluorogenic rt | 21 |
conserved region | 21 |
bp fragment | 21 |
universal influenza | 21 |
mouse anti | 21 |
avian reovirus | 21 |
mammalian cells | 21 |
cap gene | 21 |
transport medium | 21 |
negative sense | 21 |
tof ms | 21 |
viral titers | 21 |
cdv wild | 21 |
designed using | 21 |
bluetongue virus | 21 |
tm columns | 21 |
neutralising mabs | 21 |
genomic sequences | 21 |
lamp assays | 21 |
significant difference | 21 |
antigen capture | 21 |
cdna synthesis | 21 |
postweaning multisystemic | 20 |
detected using | 20 |
current study | 20 |
porcine iecs | 20 |
reverse transcribed | 20 |
analyzed using | 20 |
one sample | 20 |
rna standards | 20 |
viral particles | 20 |
pedv specific | 20 |
rabbit anti | 20 |
pbs containing | 20 |
cov igg | 20 |
human serum | 20 |
considered positive | 20 |
conserved regions | 20 |
south korea | 20 |
curve analysis | 20 |
dnase i | 20 |
european standard | 20 |
extracted using | 20 |
leukemia virus | 20 |
total volume | 20 |
cells transfected | 20 |
western immunoblot | 20 |
field samples | 20 |
vaccine strain | 20 |
north american | 20 |
taqman real | 20 |
fcovs type | 20 |
rapid identification | 20 |
dna vaccines | 20 |
rna templates | 20 |
research institute | 20 |
stool specimens | 20 |
results suggest | 20 |
important role | 20 |
highly purified | 19 |
three different | 19 |
antibody responses | 19 |
higher sensitivity | 19 |
thermal cycler | 19 |
antibody test | 19 |
antibody response | 19 |
alignment search | 19 |
acute gastroenteritis | 19 |
pcr analysis | 19 |
respiratory viral | 19 |
dna sequencing | 19 |
specific pathogen | 19 |
viral cdna | 19 |
independent experiments | 19 |
previously published | 19 |
multiplex method | 19 |
assay developed | 19 |
sequence data | 19 |
specimens collected | 19 |
results indicate | 19 |
ebola virus | 19 |
ftdrp assay | 19 |
one step | 19 |
hnov gii | 19 |
determine whether | 19 |
diagnostic tests | 19 |
preparation method | 19 |
lassa virus | 19 |
mouse igg | 18 |
diagnostic tool | 18 |
field strains | 18 |
realtime rt | 18 |
results indicated | 18 |
abi prism | 18 |
healthy cats | 18 |
innate immune | 18 |
japanese encephalitis | 18 |
hemagglutinating encephalomyelitis | 18 |
amplified products | 18 |
clinical sample | 18 |
dna copies | 18 |
fisher scientific | 18 |
diagnostic test | 18 |
detection method | 18 |
pfu ml | 18 |
assay described | 18 |
coli bl | 18 |
secondary antibody | 18 |
coronavirus strains | 18 |
van der | 18 |
neutralizing antibody | 18 |
equine sera | 18 |
amplification reaction | 18 |
melting temperature | 18 |
house assays | 18 |
pcr reactions | 18 |
kit assay | 18 |
neutralisation test | 18 |
melting curve | 18 |
method described | 18 |
matrix gene | 18 |
egyptian variant | 18 |
feline coronaviruses | 18 |
fta card | 18 |
previous study | 18 |
recovery efficiency | 18 |
parainfluenza viruses | 18 |
flk blv | 18 |
widely used | 18 |
viruses including | 17 |
cov rna | 17 |
rna transcript | 17 |
less sensitive | 17 |
human immunodeficiency | 17 |
samples obtained | 17 |
family coronaviridae | 17 |
clart pneumovir | 17 |
infected shrimp | 17 |
using primer | 17 |
young children | 17 |
ha gene | 17 |
kidney cell | 17 |
immunodeficiency virus | 17 |
chicken eggs | 17 |
performed according | 17 |
acid residues | 17 |
pcr master | 17 |
two groups | 17 |
mm mgcl | 17 |
culture supernatants | 17 |
virulent strains | 17 |
green fluorescence | 17 |
lysis buffer | 17 |
probe sequences | 17 |
yellow head | 17 |
qiaamp viral | 17 |
viral rnas | 17 |
fetal bovine | 17 |
cultured cells | 17 |
control group | 17 |
also tested | 17 |
probes used | 17 |
tracheal swabs | 17 |
transcriptase polymerase | 17 |
per well | 17 |
spike gene | 17 |
positive controls | 17 |
two samples | 17 |
ic assay | 17 |
genbank database | 17 |
disease severity | 17 |
reading frame | 17 |
statistically significant | 17 |
vero cell | 17 |
sg cells | 17 |
thermo fisher | 17 |
denv infection | 17 |
duplex assay | 17 |
serial dilution | 17 |
cost effective | 17 |
rna mini | 17 |
evaluated using | 17 |
rt real | 17 |
guinea pig | 17 |
culture medium | 17 |
silico sensitivity | 17 |
quartz crystal | 17 |
pcr primers | 17 |
postchallenge day | 17 |
step real | 17 |
per ml | 17 |
virus types | 17 |
mm nacl | 17 |
mouth disease | 17 |
murine coronavirus | 16 |
even though | 16 |
sensitive method | 16 |
copies rna | 16 |
clinical symptoms | 16 |
acid extraction | 16 |
north america | 16 |
false positives | 16 |
threshold value | 16 |
low viral | 16 |
virus using | 16 |
reaction assay | 16 |
positive sense | 16 |
transcription pcr | 16 |
min followed | 16 |
early diagnosis | 16 |
pcr detected | 16 |
cov infected | 16 |
severe disease | 16 |
cycle threshold | 16 |
statistical analysis | 16 |
virus recovery | 16 |
purified sars | 16 |
cycle sequencing | 16 |
viral shedding | 16 |
head virus | 16 |
relatively low | 16 |
infectious disease | 16 |
elisa kit | 16 |
lna assay | 16 |
lamp products | 16 |
rnase inhibitor | 16 |
large scale | 16 |
developed rt | 16 |
significant differences | 16 |
using two | 16 |
molecular epidemiology | 16 |
viral antigen | 16 |
siga positive | 16 |
cell lysate | 16 |
coronavirus spike | 16 |
cells expressing | 16 |
qcm biosensor | 16 |
respiratory diseases | 16 |
hcov oc | 16 |
two viruses | 16 |
quantitative detection | 16 |
positive rate | 16 |
diagnostic sensitivity | 16 |
enzyme immunoassay | 16 |
nested primers | 15 |
induced apoptosis | 15 |
three samples | 15 |
commonly used | 15 |
ge pfu | 15 |
tested negative | 15 |
bovine herpesvirus | 15 |
samples using | 15 |
viral respiratory | 15 |
rt step | 15 |
early detection | 15 |
highly contagious | 15 |
calf serum | 15 |
using different | 15 |
supplementary data | 15 |
specific antibody | 15 |
rsv lna | 15 |
rna copy | 15 |
arv replication | 15 |
respiratory illness | 15 |
field isolates | 15 |
coronavirus nl | 15 |
throat swabs | 15 |
respiratory infection | 15 |
ccov strains | 15 |
ns gene | 15 |
st cells | 15 |
culture method | 15 |
copies ng | 15 |
immune system | 15 |
serum sample | 15 |
viral titer | 15 |
silico validation | 15 |
specific products | 15 |
hospitalized children | 15 |
two separate | 15 |
like virus | 15 |
time qt | 15 |
genotype isolates | 15 |
serotype specific | 15 |
human influenza | 15 |
infected patients | 15 |
rsv rna | 15 |
coronavirus rna | 15 |
fluorescence intensity | 15 |
two assays | 15 |
eid ml | 15 |
positive serum | 15 |
immunochromatographic test | 15 |
probe binding | 15 |
cytopathic effects | 15 |
lassa fever | 15 |
polarized calu | 15 |
rad laboratories | 15 |
using rna | 15 |
reverse primers | 15 |
molecular diagnostics | 15 |
results demonstrated | 15 |
small interfering | 15 |
mixture contained | 15 |
end point | 14 |
similar results | 14 |
viruses tested | 14 |
step step | 14 |
novel real | 14 |
large number | 14 |
diagnostic method | 14 |
coronavirus type | 14 |
recombinant plasmid | 14 |
oseltamivir resistant | 14 |
positive sera | 14 |
may also | 14 |
minor groove | 14 |
based real | 14 |
acute phase | 14 |
fecal specimens | 14 |
binding sites | 14 |
cycling conditions | 14 |
optical density | 14 |
multiple sequence | 14 |
env gene | 14 |
amplification curves | 14 |
fluorescence signal | 14 |
interfering rnas | 14 |
ck cells | 14 |
cells using | 14 |
osel res | 14 |
coding region | 14 |
coronavirus associated | 14 |
high throughput | 14 |
detection kit | 14 |
vitro transcripts | 14 |
years old | 14 |
vaccine development | 14 |
viral diarrhoea | 14 |
coronavirus infections | 14 |
body fluids | 14 |
large amounts | 14 |
membrane proteins | 14 |
detect sars | 14 |
scfv antibodies | 14 |
detection rates | 14 |
peptides derived | 14 |
corresponding author | 14 |
viral dna | 14 |
low sensitivity | 14 |
four different | 14 |
infected pigs | 14 |
mm dntps | 13 |
osel sen | 13 |
time points | 13 |
rights reserved | 13 |
monkey kidney | 13 |
groove binder | 13 |
new assay | 13 |
software version | 13 |
herpes simplex | 13 |
containing mm | 13 |
sv tag | 13 |
technical assistance | 13 |
reading frames | 13 |
dna virus | 13 |
structural genes | 13 |
molecular tests | 13 |
human enteric | 13 |
phosphate buffered | 13 |
artificial sample | 13 |
thank dr | 13 |
kappa statistic | 13 |
new coronavirus | 13 |
viruses isolated | 13 |
triplex real | 13 |
quantified using | 13 |
reference sequence | 13 |
variant ii | 13 |
conventional one | 13 |
stranded dna | 13 |
plasma samples | 13 |
enteric pathogens | 13 |
consensus primers | 13 |
clinical sign | 13 |
cells infected | 13 |
generated using | 13 |
sequence detection | 13 |
sirna scr | 13 |
first report | 13 |
three independent | 13 |
ibv infection | 13 |
embryonated chicken | 13 |
ethics committee | 13 |
yellow fever | 13 |
norwalk virus | 13 |
normal human | 13 |
phage display | 13 |
positive colostrum | 13 |
without filtration | 13 |
hemorrhagic fever | 13 |
pcv infection | 13 |
node cells | 13 |
different concentrations | 13 |
matrix protein | 13 |
samples included | 13 |
contact times | 13 |
intestinal epithelial | 13 |
house developed | 13 |
viral nucleic | 13 |
crystal microbalance | 13 |
pcv capsid | 13 |
blot analysis | 13 |
cvb replicon | 13 |
linear regression | 13 |
acid sequences | 13 |
body cavity | 13 |
homologous recombination | 13 |
full length | 13 |
antiviral activity | 13 |
lymphoproliferative responses | 13 |
like ccovs | 13 |
spike glycoprotein | 13 |
diagnostic assays | 13 |
upper respiratory | 13 |
assay based | 13 |
like viruses | 13 |
organ samples | 13 |
nasal swab | 13 |
viral genomic | 13 |
cells co | 13 |
enveloped viruses | 13 |
species identification | 13 |
developed sample | 13 |
prrsv rna | 13 |
genetic characterization | 13 |
gene encoding | 13 |
image cytometer | 13 |
environmental surfaces | 13 |
based assay | 13 |
prv ge | 13 |
cross reactivity | 12 |
hepatitis virus | 12 |
bat cell | 12 |
viral strains | 12 |
dnase treatment | 12 |
incubation time | 12 |
viruses detected | 12 |
high viral | 12 |
lymphoid tissues | 12 |
three genotypes | 12 |
ferret lymph | 12 |
fcov type | 12 |
virus particles | 12 |
tgev rna | 12 |
washed twice | 12 |
fluorescent signal | 12 |
online version | 12 |
gel extraction | 12 |
length cdna | 12 |
resistant influenza | 12 |
low levels | 12 |
primer sequences | 12 |
embryonated eggs | 12 |
children hospitalized | 12 |
gene fragment | 12 |
time polymerase | 12 |
superscript iii | 12 |
ns antigen | 12 |
influenza season | 12 |
primer pairs | 12 |
virus shedding | 12 |
isolation kit | 12 |
gapdh mrna | 12 |
interferon beta | 12 |
na gene | 12 |
recombinant protein | 12 |
software package | 12 |
fluorescence microscope | 12 |
method using | 12 |
causative agent | 12 |
specific amplification | 12 |
also observed | 12 |
also detected | 12 |
forming units | 12 |
virus dilution | 12 |
sen probe | 12 |
pcr based | 12 |
coronaviridae family | 12 |
mm kcl | 12 |
hemagglutinin gene | 12 |
triplex assay | 12 |
human cells | 12 |
lamp primers | 12 |
hong kong | 12 |
coronavirus infectious | 12 |
persistently infected | 12 |
retention rates | 12 |
assay cvs | 12 |
aligned using | 12 |
blood mononuclear | 12 |
reaction conditions | 12 |
biotech co | 12 |
control samples | 12 |
two methods | 12 |
annealing temperature | 12 |
animal species | 12 |
primer express | 12 |
pcr positive | 12 |
lamp reactions | 12 |
fetal calf | 12 |
diagnostic tools | 12 |
antibodies directed | 12 |
colloidal gold | 12 |
done using | 12 |
rna kit | 12 |
viral genomes | 12 |
blocking elisa | 12 |
porcine transmissible | 12 |
detection mix | 12 |
new zealand | 12 |
ev pev | 12 |
multiplex assays | 12 |
analysis showed | 12 |
house assay | 12 |
tract infection | 12 |
bovine leukemia | 12 |
time detection | 12 |
res probe | 12 |
melting point | 12 |
ns serotype | 12 |
ns ag | 12 |
binding site | 12 |
previously reported | 12 |
molecular diagnostic | 12 |
pigs inoculated | 12 |
dna samples | 12 |
ms analysis | 12 |
high prevalence | 12 |
culture plates | 11 |
feline enteric | 11 |
intensive care | 11 |
affinity chromatography | 11 |
molecular weight | 11 |
test kit | 11 |
epithelial cell | 11 |
designed based | 11 |
type prv | 11 |
direct detection | 11 |
true positive | 11 |
diagnostic laboratory | 11 |
porcine hemagglutinating | 11 |
cells ml | 11 |
strain vaccine | 11 |
nv rna | 11 |
buffer containing | 11 |
ndv strains | 11 |
pcr efficiency | 11 |
cleavage site | 11 |
qpcr assay | 11 |
standard deviations | 11 |
vitro rna | 11 |
cell debris | 11 |
nested polymerase | 11 |
nitrocellulose membrane | 11 |
amplified product | 11 |
takara biotechnology | 11 |
developed using | 11 |
human rhinovirus | 11 |
pedv strains | 11 |
culture collection | 11 |
cov strain | 11 |
flow cytometric | 11 |
tube rt | 11 |
elisa using | 11 |
inner primers | 11 |
nervous system | 11 |
calf diarrhea | 11 |
found positive | 11 |
avian coronavirus | 11 |
assay targeting | 11 |
house rtrt | 11 |
coronavirus nucleocapsid | 11 |
lymphocyte proliferative | 11 |
first strand | 11 |
based amplification | 11 |
specific pcr | 11 |
accession numbers | 11 |
cell surface | 11 |
carbonate buffer | 11 |
essential medium | 11 |
cov rt | 11 |
based methods | 11 |
hek cells | 11 |
based rt | 11 |
dual infections | 11 |
cycle number | 11 |
denaturation step | 11 |
confidence interval | 11 |
genetic diversity | 11 |
conjunctival swab | 11 |
coronavirus oc | 11 |
type virus | 11 |
easy vector | 11 |
firefly luciferase | 11 |
ee ratio | 11 |
sample collection | 11 |
variant strain | 11 |
high degree | 11 |
cdv vaccine | 11 |
loop mediated | 11 |
two independent | 11 |
serological assays | 11 |
specific monoclonal | 11 |
obtained using | 11 |
viral stock | 11 |
restriction enzyme | 11 |
newly developed | 11 |
viral suspension | 11 |
rabies virus | 11 |
post infection | 11 |
test strip | 11 |
statistical significance | 11 |
virus serotypes | 11 |
inactivated sars | 11 |
enrichment method | 11 |
rna standard | 11 |
expression system | 11 |
tipped applicators | 11 |
od nm | 11 |
reaction mixtures | 11 |
indirect immunofluorescence | 11 |
sd bioline | 11 |
ion torrent | 11 |
second round | 11 |
protein genes | 11 |
positive using | 11 |
methods used | 11 |
reagent set | 11 |
serum albumin | 11 |
primers defined | 11 |
host range | 11 |
order nidovirales | 11 |
rna samples | 11 |
virulent strain | 11 |
neutralization test | 11 |
recombinant baculovirus | 11 |
protective immunity | 11 |
taq dna | 11 |
sense primer | 11 |
test results | 11 |
different swabs | 11 |
reaction time | 11 |
equal volume | 11 |
origin influenza | 11 |
target gene | 11 |
thermal cycling | 11 |
housekeeping gene | 11 |
central nervous | 11 |
polyacrylamide gel | 11 |
day post | 10 |
lyssa irus | 10 |
reported previously | 10 |
mrna copies | 10 |
analytical specificity | 10 |
eggs inoculated | 10 |
simple method | 10 |
viral protein | 10 |
mouse hepatitis | 10 |
acute lower | 10 |
tracheal washes | 10 |
pcr protocol | 10 |
infected cats | 10 |
published sequence | 10 |
detection using | 10 |
table comparison | 10 |
conjugate pad | 10 |
specimen collection | 10 |
taura syndrome | 10 |
green chemistry | 10 |
group i | 10 |
highly pathogenic | 10 |
signaling pathways | 10 |
correlation coefficient | 10 |
sars patient | 10 |
virus neutralization | 10 |
standard plasmid | 10 |
thermofisher scientific | 10 |
isothermal mastermix | 10 |
microtiter plates | 10 |
virus discovery | 10 |
currently circulating | 10 |
human adenovirus | 10 |
pcr conditions | 10 |
four viruses | 10 |
dengue viruses | 10 |
template rna | 10 |
care unit | 10 |
relative abundance | 10 |
corbett research | 10 |
differentially expressed | 10 |
dna extraction | 10 |
multiplex rtpcr | 10 |
dpo assay | 10 |
rv detection | 10 |
environmental conditions | 10 |
purified virus | 10 |
outer primers | 10 |
log reduction | 10 |
developing countries | 10 |
laboratories srl | 10 |
binding assay | 10 |
taqman rrt | 10 |
different methods | 10 |
time taqman | 10 |
enrichment techniques | 10 |
neuraminidase inhibitor | 10 |
strain josiah | 10 |
neuraminidase inhibitors | 10 |
human norovirus | 10 |
volume containing | 10 |
reference standard | 10 |
mm edta | 10 |
american type | 10 |
using virus | 10 |
routine diagnostic | 10 |
kidney cells | 10 |
rdrp gene | 10 |
vitro model | 10 |
amplified using | 10 |
unpublished data | 10 |
tested samples | 10 |
leader sequence | 10 |
per primer | 10 |
pediatric patients | 10 |
routinely used | 10 |
detection tests | 10 |
bovine enteric | 10 |
one hundred | 10 |
reporter gene | 10 |
dissociation curve | 10 |
dugbe virus | 10 |
cov spike | 10 |
like ccov | 10 |
infectious agents | 10 |
ebov makona | 10 |
assay performance | 10 |
luciferase reporter | 10 |
rsv positive | 10 |
human rhinoviruses | 10 |
penaeus monodon | 10 |
trachomatis assay | 10 |
assay will | 10 |
standard dilutions | 10 |
control measures | 10 |
resistance testing | 10 |
fecal suspension | 10 |
porcine coronaviruses | 10 |
using monoclonal | 10 |
nf en | 10 |
chain variable | 10 |
coli cells | 10 |
capsid proteins | 10 |
three multiplex | 10 |
primary antibody | 10 |
complete genome | 10 |
tract disease | 10 |
sars outbreak | 10 |
cell types | 10 |
primers targeting | 10 |
porcine samples | 10 |
mixture containing | 10 |
based assays | 10 |
mrna levels | 10 |
assay reproducibility | 10 |
bicistronic dna | 10 |
useful tool | 10 |
visual detection | 10 |
viral species | 10 |
developed assay | 10 |
infective dose | 10 |
negative result | 10 |
template controls | 10 |
der hoek | 10 |
high pure | 10 |
molecular size | 10 |
sample volume | 10 |
geometric mean | 10 |
random primer | 10 |
highly reproducible | 10 |
nasal irrigation | 10 |
lateral flow | 10 |
simplex virus | 10 |
water bath | 10 |
endpoint detection | 10 |
also included | 10 |
quantitative reverse | 10 |
coronavirus isolates | 10 |
clinical presentation | 10 |
recombinase polymerase | 10 |
polymerase amplification | 10 |
biological samples | 10 |
ftdrp assays | 10 |
another study | 10 |
cdc real | 10 |
type csfv | 10 |
genome sequences | 10 |
different viruses | 10 |
may result | 9 |
seeding density | 9 |
time point | 9 |
phylogenetic tree | 9 |
different genotypes | 9 |
recent years | 9 |
loop primers | 9 |
pmk cells | 9 |
gibco brl | 9 |
recombinant vaccinia | 9 |
animal care | 9 |
using rt | 9 |
type culture | 9 |
like particle | 9 |
taken together | 9 |
dna viruses | 9 |
live virus | 9 |
duplex rrt | 9 |
phosphate buffer | 9 |
human sars | 9 |
purification kit | 9 |
concentrations ranging | 9 |
tenfold serial | 9 |
plasmid containing | 9 |
etiological agent | 9 |
detection sensitivity | 9 |
prospective study | 9 |
assay may | 9 |
converting enzyme | 9 |
duplex rt | 9 |
del cane | 9 |
blv antigen | 9 |
coronavirus membrane | 9 |
virus control | 9 |
rpa assay | 9 |
reporter dye | 9 |
type strain | 9 |
hospitalised children | 9 |
celigo image | 9 |
gastroenteritis coronavirus | 9 |
bursal disease | 9 |
see section | 9 |
bioline norovirus | 9 |
plasmid pires | 9 |
reaction efficiency | 9 |
rna extract | 9 |
highest dilution | 9 |
fluorescence microscopy | 9 |
steel surfaces | 9 |
mean recovery | 9 |
sampling method | 9 |
positive according | 9 |
genetic analysis | 9 |
using sybr | 9 |
reaction assays | 9 |
extraction method | 9 |
ibv vaccine | 9 |
first time | 9 |
values obtained | 9 |
rt reaction | 9 |
early stage | 9 |
first step | 9 |
igg antibody | 9 |
isothermal conditions | 9 |
functional receptor | 9 |
neuraminidase gene | 9 |
step quantitative | 9 |
assay showed | 9 |
increasing number | 9 |
expected size | 9 |
icg test | 9 |
priming oligonucleotide | 9 |
days later | 9 |
probe used | 9 |
nucleotide substitutions | 9 |
nhbe cells | 9 |
rna production | 9 |
pfu ratio | 9 |
virus subtype | 9 |
antibody production | 9 |
following infection | 9 |
molecular diagnosis | 9 |
natural infection | 9 |
random hexamers | 9 |
national center | 9 |
porcine circoviruses | 9 |
elisa based | 9 |
strain bj | 9 |
dna rna | 9 |
env genes | 9 |
ibv genome | 9 |
infection using | 9 |
using trizol | 9 |
apical surface | 9 |
pigs infected | 9 |
school children | 9 |
cavity effusions | 9 |
target sequences | 9 |
secondary structure | 9 |
maternal antibodies | 9 |
target genes | 9 |
canine herpesvirus | 9 |
quality control | 9 |
neutralization assay | 9 |
nine samples | 9 |
corona virus | 9 |
nested multiplex | 9 |
onto fta | 9 |
highly infectious | 9 |
primers specific | 9 |
high level | 9 |
disease viruses | 9 |
mail address | 9 |
recombinant plasmids | 9 |
using multiplex | 9 |
per assay | 9 |
ftdrp rv | 9 |
target cells | 9 |
authors declare | 9 |
reaction buffer | 9 |
thermal profile | 9 |
protein expressed | 9 |
accurate diagnosis | 9 |
diagnostic assay | 9 |
ifn beta | 9 |
virus entry | 9 |
polymerase gene | 9 |
probe sets | 9 |
van rijn | 9 |
rna levels | 9 |
proliferative responses | 9 |
clinical laboratory | 9 |
persistent infection | 9 |
using specific | 9 |
infectious bursal | 9 |
conjunctival swabs | 9 |
dhinakar raj | 9 |
high mortality | 9 |
biotechnology co | 9 |
major structural | 9 |
negative group | 9 |
test result | 9 |
absolute quantification | 9 |
inoculum levels | 9 |
scale rna | 9 |
will allow | 9 |
atcc ccl | 9 |
measured using | 9 |
also found | 9 |
serial ten | 9 |
genetic variability | 9 |
times lower | 9 |
base differences | 9 |
elisa antigen | 9 |
single nucleotide | 9 |
using pcr | 9 |
study showed | 9 |
laboratory manual | 9 |
length ibv | 9 |
ecogpt gene | 9 |
qpcr method | 9 |
life cycle | 9 |
infected individuals | 9 |
target region | 9 |
infected chickens | 9 |
proteasome inhibitor | 9 |
fold serially | 9 |
pcv lamp | 9 |
fluorogenic pcr | 9 |
biological materials | 9 |
purified clp | 9 |
human bocavirus | 9 |
fold change | 9 |
united kingdom | 9 |
cell responses | 9 |
genome copy | 9 |
equine viral | 9 |
reverse genetics | 9 |
incubation period | 9 |
selected based | 9 |
colostrum samples | 9 |
polyester swabs | 9 |
growth medium | 9 |
sampling methods | 9 |
tqm probe | 9 |
rsv subgroups | 9 |
pedv antibodies | 9 |
specific binding | 9 |
product activity | 9 |
virus vaccine | 9 |
plaque assay | 9 |
gene specific | 9 |
rna concentration | 9 |
viruses associated | 9 |
binding activity | 8 |
like strains | 8 |
situ hybridization | 8 |
positive reaction | 8 |
single step | 8 |
new human | 8 |
new method | 8 |
low copy | 8 |
bp amplicon | 8 |
pedv strain | 8 |
veterinary medicine | 8 |
mp rt | 8 |
cell leukemia | 8 |
variability among | 8 |
transcription nested | 8 |
amplification conditions | 8 |
spike proteins | 8 |
human enterovirus | 8 |
secondary antibodies | 8 |
swine industry | 8 |
veterinary research | 8 |
infected cattle | 8 |
pig sera | 8 |
myotis polyomavirus | 8 |
pev assay | 8 |
two strains | 8 |
reference strain | 8 |
commercial rtrt | 8 |
crfk cells | 8 |
exogenous reference | 8 |
sucrose gradient | 8 |
regression analysis | 8 |
enteric coronaviruses | 8 |
cutoff value | 8 |
agricultural sciences | 8 |
coronavirus disease | 8 |
primer explorer | 8 |
genetic variation | 8 |
three replicates | 8 |
serologic assays | 8 |
fusion proteins | 8 |
topo ta | 8 |
classical rabies | 8 |
energy transfer | 8 |
viral disease | 8 |
related virus | 8 |
fcov strains | 8 |
significant reduction | 8 |
cdv strains | 8 |
compared using | 8 |
software programs | 8 |
peg nacl | 8 |
new variant | 8 |
pcr testing | 8 |
hospital serdang | 8 |
virus identification | 8 |
human noroviruses | 8 |
clinical features | 8 |
ccov infection | 8 |
among different | 8 |
blocking effect | 8 |
vaccinia viruses | 8 |
porcine viruses | 8 |
unclassified becv | 8 |
partially purified | 8 |
slightly higher | 8 |
recombinant antigen | 8 |
pathogen free | 8 |
storage conditions | 8 |
probe sequence | 8 |
fluid samples | 8 |
enteric coronavirus | 8 |
least two | 8 |
positive sample | 8 |
prepared using | 8 |
iipcr reagent | 8 |
strain elmo | 8 |
washed times | 8 |
antiseptic antiviral | 8 |
purified viral | 8 |
prototype strains | 8 |
specific sirnas | 8 |
sars virus | 8 |
pcr procedure | 8 |
i real | 8 |
uv transilluminator | 8 |
sodium fluorescein | 8 |
bright field | 8 |
conserved among | 8 |
nephropathogenic strain | 8 |
degenerated primers | 8 |
experimental infection | 8 |
causing severe | 8 |
avirulent strains | 8 |
pig anti | 8 |
qualitative rt | 8 |
regulated deps | 8 |
slightly less | 8 |
mm dtt | 8 |
negative sample | 8 |
influenza strains | 8 |
rna using | 8 |
cvb infection | 8 |
linear relationship | 8 |
throughput sequencing | 8 |
epidemiological studies | 8 |
randomly selected | 8 |
probable sars | 8 |
canine adenovirus | 8 |
human parainfluenza | 8 |
analysis using | 8 |
first days | 8 |
cell monolayers | 8 |
length genome | 8 |
whole virus | 8 |
genotype strains | 8 |
amplification cycles | 8 |
per vial | 8 |
bal fluid | 8 |
live attenuated | 8 |
envelope protein | 8 |
newly discovered | 8 |
single stranded | 8 |
results show | 8 |
virology laboratory | 8 |
new virus | 8 |
antibody detection | 8 |
enteric virus | 8 |
variant strains | 8 |
virus neutralizing | 8 |
gradient centrifugation | 8 |
green pcr | 8 |
blood donors | 8 |
clinical management | 8 |
house singleplex | 8 |
increased sensitivity | 8 |
diagnostic purposes | 8 |
capsid expresser | 8 |
four primers | 8 |
patient management | 8 |
viral envelope | 8 |
orthopoxvirus dna | 8 |
american swine | 8 |
pcv antibodies | 8 |
allantoic fluids | 8 |
coronavirus causing | 8 |
virus genomes | 8 |
dogs suspected | 8 |
novel swine | 8 |
low concentrations | 8 |
england biolabs | 8 |
short time | 8 |
human disease | 8 |
amplification product | 8 |
point mutations | 8 |
tracheal organ | 8 |
neutralization assays | 8 |
nucleocapsid gene | 8 |
mean fluorescence | 8 |
fluorogenic assay | 8 |
blood cells | 8 |
image cytometry | 8 |
test line | 8 |
ecl detection | 8 |
quantitative assay | 8 |
sample type | 8 |
sera raised | 8 |
nonstructural protein | 8 |
described herein | 8 |
standard nf | 8 |
two distinct | 8 |
loaded onto | 8 |
animal disease | 8 |
grant number | 8 |
specific product | 8 |
plasmid standard | 8 |
deep sequencing | 8 |
virus date | 8 |
gene amplification | 8 |
pdu ml | 8 |
commercial rna | 8 |
information processing | 8 |
based vaccines | 8 |
sterile distilled | 8 |
protein fragments | 8 |
ftdrp ev | 8 |
rna per | 8 |
nucleotide sequencing | 8 |
unexposed pigs | 8 |
rnase free | 8 |
amplitaq gold | 8 |
blv provirus | 8 |
ta cloning | 8 |
guangdong province | 8 |
following formula | 8 |
samples used | 8 |
conjugated anti | 8 |
national health | 8 |
strand rna | 8 |
roche diagnostics | 8 |
rna sample | 8 |
pcr target | 8 |
cotton tipped | 8 |
pcr thermocycler | 8 |
cotton swab | 8 |
suspected patients | 8 |
mixed infections | 8 |
rt pcr | 8 |
single assay | 8 |
antiviral drugs | 8 |
transport mediums | 8 |
turnaround time | 8 |
potential use | 8 |
fragment length | 8 |
oligonucleotide system | 8 |
drying time | 8 |
virus titer | 8 |
endogenous control | 8 |
new england | 8 |
transcription step | 8 |
suckling piglets | 8 |
per sample | 8 |
specific real | 8 |
viral mrna | 8 |
molecular biology | 8 |
standard dna | 8 |
molecular cloning | 8 |
blast search | 8 |
assays used | 8 |
line po | 8 |
control rna | 8 |
scr env | 8 |
dual priming | 8 |
ace antibody | 8 |
reliable method | 8 |
coated quartz | 8 |
highly variable | 8 |
parvovirus dna | 8 |
extension step | 8 |
tokyo metropolitan | 8 |
bp product | 8 |
ex vivo | 8 |
pcr diagnostics | 8 |
supplementary table | 8 |
house ev | 8 |
virus stock | 8 |
san diego | 7 |
nasopharyngeal aspirates | 7 |
detect viruses | 7 |
vp gene | 7 |
sick children | 7 |
strain cdv | 7 |
two commercial | 7 |
veterinary diagnostic | 7 |
amplification using | 7 |
enterovirus serotypes | 7 |
vaccinated exposed | 7 |
subgenomic mrna | 7 |
antibody immobilization | 7 |
absorbance values | 7 |
viral gene | 7 |
rapid molecular | 7 |
using anti | 7 |
sequencing method | 7 |
least days | 7 |
rna detection | 7 |
vector system | 7 |
od values | 7 |
pcr amplicons | 7 |
polyclonal antibodies | 7 |
bovine torovirus | 7 |
laboratory diagnostic | 7 |
study describes | 7 |
na assay | 7 |
fluorescent dye | 7 |
cap proteins | 7 |
mm phosphate | 7 |
cat populations | 7 |
proteasome pathway | 7 |
absorbance value | 7 |
minor modifications | 7 |
influenza surveillance | 7 |
pathway groups | 7 |
transcribed using | 7 |
microbiology laboratory | 7 |
percent agreement | 7 |
plaque forming | 7 |
also positive | 7 |
spectrophotometric parameters | 7 |
high fever | 7 |
healthy individuals | 7 |
template dna | 7 |
infecting cattle | 7 |
dna fragment | 7 |
kupe virus | 7 |
amplification assays | 7 |
ncr based | 7 |
small intestine | 7 |
ccov strain | 7 |
early stages | 7 |
binding region | 7 |
iipcr assay | 7 |
combined rt | 7 |
tsv infected | 7 |
throat swab | 7 |
pedv viral | 7 |
identification limit | 7 |
three clones | 7 |
conducted using | 7 |
direct immunofluorescence | 7 |
ib virus | 7 |
also performed | 7 |
cov cdna | 7 |
mrna loads | 7 |
cowpox virus | 7 |
ns rt | 7 |
cellular immune | 7 |
cell cycle | 7 |
od value | 7 |
east respiratory | 7 |
human caliciviruses | 7 |
cdna template | 7 |
virus release | 7 |
acquired pneumonia | 7 |
first line | 7 |
chinese academy | 7 |
like pestiviruses | 7 |
infection system | 7 |
early phase | 7 |
rnai technology | 7 |
different viral | 7 |
sars positive | 7 |
two sets | 7 |
initial rna | 7 |
quantitative analysis | 7 |
containing ml | 7 |
testing samples | 7 |
viruses used | 7 |
antisense primer | 7 |
norovirus infection | 7 |
specific lesions | 7 |
tracheal swab | 7 |
tcid cdv | 7 |
vitro assembly | 7 |
control antigen | 7 |
three rt | 7 |
four sars | 7 |
excellent technical | 7 |
limited number | 7 |
first round | 7 |
virus vaccines | 7 |
pestiviruses infecting | 7 |
virus antibodies | 7 |
assay specificity | 7 |
elution buffers | 7 |
including influenza | 7 |
enteric caliciviruses | 7 |
fold diluted | 7 |
assays based | 7 |
rna extracts | 7 |
children admitted | 7 |
bromide staining | 7 |
bicistronic plasmid | 7 |
cdv cell | 7 |
dna template | 7 |
murine encephalomyelitis | 7 |
proteasome inhibition | 7 |
direct tm | 7 |
health commission | 7 |
assay coefficient | 7 |
natural science | 7 |
analysis revealed | 7 |
fold lower | 7 |
refractory mutation | 7 |
time amplification | 7 |
single tube | 7 |
virus infectivity | 7 |
assays described | 7 |
fluorogenic reverse | 7 |
different animal | 7 |
thermo scientific | 7 |
different amounts | 7 |
ferret model | 7 |
commercial elisa | 7 |
acid change | 7 |
strand cdna | 7 |
ccov rna | 7 |
pcr targeting | 7 |
production system | 7 |
stock preparation | 7 |
semen samples | 7 |
pcr multiplex | 7 |
reaction containing | 7 |
restriction sites | 7 |
ex taq | 7 |
significantly different | 7 |
insect cells | 7 |
without sirna | 7 |
restriction fragment | 7 |
control sirna | 7 |
detect antibodies | 7 |
glycine buffer | 7 |
based method | 7 |
postinoculation days | 7 |
growth curve | 7 |
single peak | 7 |
length polymorphism | 7 |
cells per | 7 |
dna content | 7 |
lasv strain | 7 |
identified peptides | 7 |
lagos bat | 7 |
pfu per | 7 |
swab types | 7 |
genus alphacoronavirus | 7 |
nucleoprotein gene | 7 |
ib primer | 7 |
single round | 7 |
quantitative results | 7 |
cell pellet | 7 |
commercial kits | 7 |
pneumovir test | 7 |
clinical disease | 7 |
borne viruses | 7 |
positive clinical | 7 |
etiologic agent | 7 |
competent escherichia | 7 |
rapid screening | 7 |
see table | 7 |
mean value | 7 |
several days | 7 |
amv reverse | 7 |
virus detected | 7 |
gb acc | 7 |
purified recombinant | 7 |
bovine leukosis | 7 |
human infection | 7 |
challenge day | 7 |
samples detected | 7 |
genome organization | 7 |
samples also | 7 |
mucosal surfaces | 7 |
viral families | 7 |
polarized epithelial | 7 |
oligonucleotide primer | 7 |
method detected | 7 |
exposed pigs | 7 |
virus rapid | 7 |
enteric disease | 7 |
transcription reaction | 7 |
mdck cells | 7 |
annealing temperatures | 7 |
qiaquick gel | 7 |
true positives | 7 |
denv ns | 7 |
sequence reads | 7 |
pcr developed | 7 |
showed high | 7 |
possible cause | 7 |
retention rate | 7 |
sn assay | 7 |
used widely | 7 |
biotechnology information | 7 |
specimens positive | 7 |
tertiary care | 7 |
mycoplasma pneumoniae | 7 |
tissue kit | 7 |
different virus | 7 |
fipv df | 7 |
study design | 7 |
swab sample | 7 |
base pair | 7 |
virus concentration | 7 |
reference rna | 7 |
microneutralization assay | 7 |
encephalomyocarditis virus | 7 |
influenza pandemic | 7 |
blood lymphocytes | 7 |
mixed samples | 7 |
virological diagnostics | 7 |
bst dna | 7 |
pair ccov | 7 |
step multiplex | 7 |
following conditions | 7 |
treated water | 7 |
nasopharyngeal swabs | 7 |
extraction step | 7 |
genomic sequence | 7 |
various concentrations | 7 |
clinical validation | 7 |
palo alto | 7 |
env co | 7 |
novel pandemic | 7 |
tm sybr | 7 |
infection control | 7 |
fecal suspensions | 7 |
sample containing | 7 |
fluorescent signals | 7 |
uv light | 7 |
antibody levels | 7 |
true negative | 7 |
orf ab | 7 |
conventional viral | 7 |
stranded positive | 7 |
neurological signs | 7 |
mk cells | 7 |
novel influenza | 7 |
untranslated region | 7 |
acute infection | 7 |
sample pad | 7 |
sodium dodecyl | 7 |
endogenous reference | 7 |
adenovirus type | 7 |
virus species | 7 |
metropolitan institute | 7 |
horseradish peroxidase | 7 |
cdna within | 7 |
informed consent | 7 |
actin genes | 7 |
screening assay | 7 |
recombinant proteins | 7 |
human pbmcs | 7 |
wnv pcr | 7 |
paper describes | 7 |
viral arteritis | 7 |
antigen production | 7 |
mean cpm | 7 |
respiratory panel | 7 |
different respiratory | 7 |
sample types | 7 |
molecules tested | 7 |
surfaces using | 7 |
one mismatch | 7 |
elisa plates | 7 |
previous report | 7 |
circulating strains | 7 |
antibody libraries | 7 |
mucosal immune | 7 |
lymphocyte proliferation | 7 |
false negatives | 7 |
mutation system | 7 |
using one | 7 |
health research | 7 |
rapid diagnostic | 7 |
based multiplex | 7 |
color change | 7 |
roche assay | 7 |
viral entry | 7 |
lung tissue | 7 |
pure rna | 7 |
sequences used | 7 |
personal communication | 7 |
diagnostic accuracy | 7 |
target rna | 7 |
diagnostic testing | 7 |
rsv strains | 7 |
science foundation | 7 |
mrc cells | 7 |
middle east | 7 |
viruses circulating | 7 |
duplex taqman | 7 |
bat species | 7 |
newly identified | 7 |
capture antibody | 7 |
pe applied | 7 |
blot assay | 7 |
total sample | 7 |
pcr enzyme | 7 |
np swab | 7 |
orf encodes | 7 |
scr gag | 7 |
severe respiratory | 7 |
positive test | 7 |
perkin elmer | 7 |
pcr steps | 7 |
pharmacia biotech | 7 |
multiplex methods | 7 |
culture flasks | 7 |
loopmediated isothermal | 7 |
higher recovery | 7 |
first isolated | 7 |
samples taken | 7 |
glycoprotein gene | 7 |
electron microscope | 7 |
may cause | 7 |
ftdrp multiplex | 7 |
immunocompromised patients | 7 |
rat theilovirus | 7 |
reference method | 7 |
one virus | 7 |
luc pseudovirus | 7 |
greater sensitivity | 7 |
test samples | 7 |
cell fusion | 7 |
african green | 7 |
clinical virology | 7 |
may explain | 7 |
bp dna | 7 |
amplification refractory | 7 |
three sets | 7 |
bronchoalveolar lavage | 7 |
positive specimens | 7 |
amplification tests | 7 |
virus transmission | 7 |
one egg | 7 |
amplification plot | 6 |
ib strains | 6 |
sequences obtained | 6 |
one test | 6 |
antibodies specific | 6 |
cells following | 6 |
cause respiratory | 6 |
nasopharyngeal aspirate | 6 |
unclassified becvs | 6 |
clinical respiratory | 6 |
microtiter plate | 6 |
reaction tube | 6 |
assay kit | 6 |
infected sf | 6 |
norovirus test | 6 |
signs method | 6 |
insulated isothermal | 6 |
repeated three | 6 |
calculated according | 6 |
based ifa | 6 |
co incubator | 6 |
clinical isolates | 6 |
normal saline | 6 |
temperature range | 6 |
resistant isolates | 6 |
chickens immunized | 6 |
avian paramyxovirus | 6 |
rv assay | 6 |
molecular weights | 6 |
health concern | 6 |
resistant pandemic | 6 |
fluorogenic probe | 6 |
melting points | 6 |
cdna preparations | 6 |
six established | 6 |
pcr result | 6 |
human lung | 6 |
national key | 6 |
igg antibodies | 6 |
four structural | 6 |
weeks post | 6 |
border disease | 6 |
heat inactivated | 6 |
test method | 6 |
serological tests | 6 |
efk clones | 6 |
ribomax tm | 6 |
lower sensitivity | 6 |
first reported | 6 |
i interferon | 6 |
whole sars | 6 |
challenge studies | 6 |
hpa coronavirus | 6 |
ferret leukocytes | 6 |
limited settings | 6 |
rabies viruses | 6 |
variant i | 6 |
enteric calicivirus | 6 |
internal reference | 6 |
nv stock | 6 |
pcr annealing | 6 |
also assessed | 6 |
amplification efficacy | 6 |
detection systems | 6 |
many countries | 6 |
enteric viral | 6 |
animal models | 6 |
agarose gels | 6 |
double distilled | 6 |
reference rnas | 6 |
biosafety level | 6 |
results using | 6 |
used according | 6 |
rabbit igg | 6 |
sequencing using | 6 |
spf pigs | 6 |
medium containing | 6 |
ascites fluid | 6 |
data associated | 6 |
associated virus | 6 |
cov viral | 6 |
control cells | 6 |
cdna standards | 6 |
apoptosis induction | 6 |
culture methods | 6 |
specific assay | 6 |
complete genomic | 6 |
gave similar | 6 |
containing pcr | 6 |
stimulated wells | 6 |
different geographical | 6 |
independent assays | 6 |
assay detection | 6 |
reaction contained | 6 |
novel tool | 6 |
mediated immune | 6 |
golgi complex | 6 |
virus positive | 6 |
detect pedv | 6 |
six independent | 6 |
coronavirus rt | 6 |
samples found | 6 |
louis encephalitis | 6 |
derived peptides | 6 |
molecular mass | 6 |
conservation plot | 6 |
methods described | 6 |
rabbit sera | 6 |
peritonitis cases | 6 |
egfp gene | 6 |
small membrane | 6 |
amplification methods | 6 |
rv assays | 6 |
novel pestivirus | 6 |
strain hb | 6 |
pcr cycle | 6 |
front matter | 6 |
chicken embryos | 6 |
filter paper | 6 |
trizol reagent | 6 |
terminator cycle | 6 |
respiratory signs | 6 |
quality assessment | 6 |
ffu ml | 6 |
nd nd | 6 |
ee indices | 6 |
coating antigen | 6 |
family flaviviridae | 6 |
associated lymphoid | 6 |
method used | 6 |
specific rna | 6 |
sequence database | 6 |
orthopoxvirus species | 6 |
emerging viruses | 6 |
antiviral therapy | 6 |
samples submitted | 6 |
cell clones | 6 |
previous reports | 6 |
membrane fusion | 6 |
genus pestivirus | 6 |
fcov mrna | 6 |
effective method | 6 |
infected dog | 6 |
variable regions | 6 |
ml reaction | 6 |
relationships among | 6 |
plasmid vector | 6 |
short hairpin | 6 |
protein ns | 6 |
poultry industry | 6 |
using molecular | 6 |
specific probes | 6 |
protective immune | 6 |
dna replication | 6 |
diarrhea viruses | 6 |
test kits | 6 |
pcr inhibitors | 6 |
using single | 6 |
internal primer | 6 |
without fip | 6 |
automated sequencing | 6 |
virus culture | 6 |
directly compared | 6 |
display system | 6 |
green monkey | 6 |
antibody testing | 6 |
surface types | 6 |
mab dilution | 6 |
tgev purdue | 6 |
reducing agent | 6 |
diluted sample | 6 |
unexposed animals | 6 |
porcine deltacoronavirus | 6 |
speed centrifugation | 6 |
immunosorbent electron | 6 |
time assay | 6 |
primer mix | 6 |
isolation technique | 6 |
pcr date | 6 |
integral membrane | 6 |
infectious rna | 6 |
recombinant rna | 6 |
cells well | 6 |
barrier colony | 6 |
round infection | 6 |
perfect base | 6 |
subunit vaccine | 6 |
viral antigens | 6 |
diagnostic performance | 6 |
plasmid controls | 6 |
organismal systems | 6 |
two types | 6 |
taqman assays | 6 |
pathogenic viruses | 6 |
sodium bicarbonate | 6 |
sequencing technologies | 6 |
faecal specimens | 6 |
cho cells | 6 |
new variants | 6 |
dengue infection | 6 |
primer annealing | 6 |
purdue strain | 6 |
sequencing kit | 6 |
mutations associated | 6 |
tm one | 6 |
organ culture | 6 |
noncoding region | 6 |
several factors | 6 |
following primers | 6 |
broad range | 6 |
disease caused | 6 |
na genes | 6 |
regression curve | 6 |
amplification times | 6 |
proliferation responses | 6 |
ccv primers | 6 |
two hundred | 6 |
ccov fluorogenic | 6 |
pcr cycles | 6 |
study using | 6 |
conventional pigs | 6 |
human sera | 6 |
based sars | 6 |
elisa assay | 6 |
significantly lower | 6 |
final reaction | 6 |
electrophoresis systems | 6 |
newly emerging | 6 |
phylogenetic analyses | 6 |
nlv outbreaks | 6 |
currently available | 6 |
realart hpa | 6 |
system using | 6 |
tube containing | 6 |
pcr negative | 6 |
replicon rna | 6 |
immunochromatography test | 6 |
virus dilutions | 6 |
overall sensitivity | 6 |
sars cases | 6 |
samples showed | 6 |
following thermal | 6 |
swine viruses | 6 |
virus development | 6 |
nucleocapsid antigen | 6 |
exo probe | 6 |
pcr may | 6 |
reference ccov | 6 |
comparative sequence | 6 |
purified protein | 6 |
product tested | 6 |
several coronaviruses | 6 |
cell population | 6 |
cov assay | 6 |
sample buffer | 6 |
much higher | 6 |
cytometric analysis | 6 |
onestep rt | 6 |
point mutation | 6 |
high levels | 6 |
pedv antigen | 6 |
positive rt | 6 |
assay sensitivity | 6 |
virus persistence | 6 |
control sequences | 6 |
sequence alignments | 6 |
oc hku | 6 |
czech republic | 6 |
healthy animals | 6 |
heat inactivation | 6 |
low concentration | 6 |
nv nv | 6 |
detector antibody | 6 |
ge gene | 6 |
step sybr | 6 |
like illness | 6 |
free dnase | 6 |
cell scraping | 6 |
recombination events | 6 |
cdna clone | 6 |
thaw cycles | 6 |
cycles consisting | 6 |
monoclonal anti | 6 |
mismatch per | 6 |
assays performed | 6 |
new viruses | 6 |
three cases | 6 |
virus antigen | 6 |
nc membrane | 6 |
pcr run | 6 |
analysis indicated | 6 |
independent runs | 6 |
primer binding | 6 |
mulv reverse | 6 |
isotype control | 6 |
highly virulent | 6 |
low level | 6 |
virus titre | 6 |
using serially | 6 |
cystic fibrosis | 6 |
comparative analysis | 6 |
norovirus real | 6 |
luciferase activity | 6 |
using loop | 6 |
siga sc | 6 |
calculated based | 6 |
several species | 6 |
shell vial | 6 |
single copy | 6 |
two human | 6 |
based pcr | 6 |
use committee | 6 |
positive group | 6 |
virucidal activity | 6 |
three assays | 6 |
parental cell | 6 |
negative rt | 6 |
mrna detection | 6 |
dna plasmid | 6 |
stock solution | 6 |
ge healthcare | 6 |
fluorescence detection | 6 |
primer sequence | 6 |
passive immunization | 6 |
turkey coronavirus | 6 |
sirnas env | 6 |
based binding | 6 |
serological methods | 6 |
conjugated goat | 6 |
prokaryotic expression | 6 |
six primers | 6 |
proliferative response | 6 |
external quality | 6 |
ibv vaccines | 6 |
plasmid standards | 6 |
lower detection | 6 |
local immune | 6 |
frozen fecal | 6 |
hazara virus | 6 |
rapid immunochromatography | 6 |
detection test | 6 |
taq polymerase | 6 |
shell vials | 6 |
biological properties | 6 |
genome segment | 6 |
solution containing | 6 |
antibody tests | 6 |
bronchitis viruses | 6 |
disease complex | 6 |
university hospital | 6 |
pcr runs | 6 |
pcr protocols | 6 |
cdv field | 6 |
filmarray respiratory | 6 |
infected groups | 6 |
feline panleukopenia | 6 |
mrna rt | 6 |
detected positive | 6 |
mrna expression | 6 |
bovine leukaemia | 6 |
cultured viruses | 6 |
qiagen gmbh | 6 |
pcr techniques | 6 |
viral detection | 6 |
diarrhea specimens | 6 |
field strain | 6 |
patients infected | 6 |
primer design | 6 |
human embryonic | 6 |
visualized using | 6 |
reconstituted clinical | 6 |
different primers | 6 |
dengue fever | 6 |
resistant virus | 6 |
transmembrane protein | 6 |
virus oc | 6 |
natural host | 6 |
penaeid shrimp | 6 |
type porcine | 6 |
volume subjected | 6 |
beckman coulter | 6 |
amplification step | 6 |
annealing extension | 6 |
gov blast | 6 |
antemortem diagnosis | 6 |
cross reaction | 6 |
immunoblot analysis | 6 |
bovine pestiviruses | 6 |
ii viruses | 6 |
heat denatured | 6 |
nucleotide database | 6 |
pcr contained | 6 |
minimal essential | 6 |
capture enzyme | 6 |
medical research | 6 |
institutional review | 6 |
fluorescence signals | 6 |
available sequences | 6 |
diversity among | 6 |
assays showed | 6 |
mm sodium | 6 |
positive fecal | 6 |
common respiratory | 6 |
cellular responses | 6 |
see front | 6 |
pedv infections | 6 |
cell seeding | 6 |
linear range | 6 |
cellular rna | 6 |
cvb pseudovirus | 6 |
infection rate | 6 |
major cause | 6 |
significant increase | 6 |
known sequence | 6 |
two pairs | 6 |
values ranged | 6 |
base pairing | 6 |
using sanger | 6 |
fatal disease | 6 |
ml penicillin | 6 |
egg inoculated | 6 |
attenuated pedv | 6 |
respiratory distress | 6 |
risk factors | 6 |
amplification curve | 6 |
bd biosciences | 6 |
target rt | 6 |
universal primer | 6 |
within min | 6 |
one tube | 6 |
amplified pcr | 6 |