bigram

This is a table of type bigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

bigram	frequency
time pcr	3081
nucleic acid	2804
respiratory tract	2349
chain reaction	2319
pcr assay	2265
polymerase chain	2245
stem cell	2218
acute respiratory	2083
reverse transcription	1673
time rt	1666
respiratory viruses	1624
mg kg	1610
influenza virus	1555
respiratory syndrome	1500
pcr assays	1404
gene expression	1401
bone marrow	1368
cell transplantation	1315
cell lines	1283
novel coronavirus	1260
present study	1258
viral rna	1237
severe acute	1195
blood donors	1193
performed using	1165
viral load	1165
significantly higher	1131
clinical samples	1111
peripheral blood	1099
clinical signs	1095
control group	1088
respiratory syncytial	1061
whole blood	1038
infectious diseases	1017
syncytial virus	999
median age	994
cell culture	987
cord uid	974
doc id	974
risk factors	967
coronavirus disease	966
flow cytometry	953
virus infection	934
public health	931
influenza viruses	922
amino acid	900
significant difference	897
isothermal amplification	895
epithelial cells	890
ng ml	886
chest ct	884
pcr products	880
statistically significant	875
immune response	870
cell line	870
viral infections	865
nucleic acids	862
blood samples	847
blood transfusion	845
united states	840
multiplex pcr	827
reverse transcriptase	821
stem cells	818
real time	816
lower respiratory	808
clinical specimens	771
mean age	760
positive samples	756
study design	748
rapid detection	745
tract infections	743
red blood	739
significant differences	736
years old	730
pcr testing	727
hematopoietic stem	715
human metapneumovirus	714
nested pcr	709
one patient	702
patients received	680
mediated isothermal	661
positive results	656
respiratory virus	652
mass spectrometry	647
pcr amplification	644
results suggest	643
two groups	633
cd cells	629
overall survival	628
important role	626
extracellular vesicles	624
breast cancer	621
syndrome coronavirus	617
blood cells	610
western blot	605
respiratory infections	603
nervous system	595
respiratory disease	590
pcr results	590
test results	589
pcr test	588
electron microscopy	587
mg dl	573
copies ml	571
present document	571
summary conclusion	570
en el	569
cystic fibrosis	565
previously described	561
blood components	560
high risk	555
avian influenza	554
upper respiratory	548
viral infection	544
intensive care	544
blood donation	541
serum samples	540
commercially available	540
escherichia coli	531
cd cd	531
gel electrophoresis	523
blood products	522
infectious disease	520
previous studies	517
dna polymerase	513
tract infection	512
protein expression	512
conditioning regimen	509
body weight	509
least one	508
immune system	506
sybr green	504
pcr tests	504
tested positive	504
acquired pneumonia	502
red cell	502
applied biosystems	501
case studies	500
significantly lower	499
blood pressure	497
quantitative pcr	496
lymph node	494
blood group	492
summary conclusions	491
simultaneous detection	491
mg ml	489
blood cell	488
negative results	488
copy number	483
time reverse	481
two patients	481
background case	481
lymph nodes	477
mrna expression	473
may also	472
university hospital	466
respiratory pathogens	465
rna extraction	465
host disease	464
chronic gvhd	461
clinical features	459
sequence analysis	459
central nervous	458
staphylococcus aureus	458
pcr product	457
pg ml	457
significantly increased	455
pregnant women	454
two different	454
statistical analysis	453
cell proliferation	453
rna viruses	453
author funder	452
positive control	452
granted medrxiv	451
immune responses	450
infectious bronchitis	450
phylogenetic analysis	449
closely related	447
genomic dna	446
virus isolation	445
type i	445
acute gvhd	440
adverse events	439
retrospective study	439
agarose gel	438
data suggest	437
lung cancer	434
median time	433
blood bank	433
infected patients	433
room temperature	433
oxidative stress	433
virus detection	431
respiratory symptoms	431
clinical characteristics	431
respiratory viral	429
specific primers	429
monoclonal antibodies	429
smooth muscle	428
food safety	428
total rna	426
widely used	424
infected cells	423
results showed	422
risk factor	421
diagnostic tests	420
copyright holder	419
side effects	419
previously reported	418
results indicate	416
world health	414
human coronavirus	413
first time	412
high sensitivity	412
detection limit	410
determine whether	409
endothelial cells	408
respiratory infection	407
cell cultures	405
generation sequencing	405
clinical trials	404
negative control	403
acid amplification	401
internal control	401
hbv dna	400
hong kong	400
virus infections	398
median follow	397
case report	397
pcr reaction	397
tumor cells	397
viral loads	397
growth factor	397
fecal samples	397
wild type	395
reference genes	395
disease control	394
safety authority	394
chronic hepatitis	393
adult patients	393
tissue samples	393
european food	390
informed consent	389
medical center	388
gold standard	388
commonly used	388
design method	388
health organization	385
significantly different	384
samples collected	384
quantitative real	382
amino acids	382
renal failure	381
health care	381
version posted	376
results finding	375
study period	374
respiratory illness	374
multiplex real	373
large number	372
bronchitis virus	371
differential diagnosis	370
viral pathogens	370
increased risk	370
cancer cells	366
highly sensitive	366
young children	364
free survival	362
quality control	362
expression levels	361
cell surface	360
systematic review	360
clinical symptoms	359
wide range	359
care unit	356
significant increase	355
ct values	354
cell types	354
prostate cancer	354
pandemic influenza	353
pcr detection	352
logistic regression	351
respiratory specimens	350
transfusion medicine	349
cell death	349
nk cells	349
total number	348
significantly reduced	346
healthy controls	346
false negative	345
inflammatory response	342
pcr analysis	342
cord blood	342
viral genome	342
determined using	341
human bocavirus	341
patients undergoing	340
allogeneic hematopoietic	340
false positive	338
year old	337
hospitalized patients	337
pediatric patients	336
disease severity	335
mononuclear cells	334
monoclonal antibody	334
days post	334
acid extraction	334
three patients	332
time points	332
platelet count	331
respiratory samples	329
molecular methods	328
pacientes con	327
specific antibodies	326
nasopharyngeal swabs	326
blood donor	322
cell carcinoma	322
sample preparation	321
infectious agents	321
cohort study	321
lung disease	320
cerebrospinal fluid	319
pcr using	319
situ hybridization	318
recent years	318
analyzed using	317
porcine epidemic	317
pcr system	315
nasopharyngeal swab	315
feline infectious	314
infectious peritonitis	313
symptom onset	312
pcr positive	312
results show	311
predictive value	311
one case	311
primer pairs	311
analysis revealed	309
urinary tract	309
clinical isolates	309
cancer patients	308
cell cycle	306
prospective study	306
los pacientes	306
hcv rna	306
patients receiving	305
seasonal influenza	305
gvhd prophylaxis	303
analysis showed	303
middle east	303
patients treated	303
parainfluenza virus	303
clinical practice	302
dendritic cells	300
peer review	300
clinical diagnosis	300
kg day	300
diarrhea virus	299
spinal cord	297
renal function	297
clinical data	297
pcr method	296
complete remission	294
results obtained	294
detection methods	293
high levels	293
viral replication	293
red cells	293
streptococcus pneumoniae	292
step rt	292
recent studies	292
significantly associated	292
lung function	292
porcine circovirus	291
innate immune	290
dengue virus	289
lg ml	289
conventional rt	289
linked immunosorbent	289
type ii	289
described previously	288
three different	287
time quantitative	287
sample collection	286
molecular weight	286
patients without	285
iu ml	285
immunosorbent assay	285
clinical presentation	284
pcr kit	284
stool samples	283
positive patients	283
master mix	283
made available	282
long term	282
mouse model	281
detection rate	281
disease virus	280
diagnostic methods	280
high prevalence	279
blood cultures	279
epidemic diarrhea	278
plasma membrane	278
measured using	276
en pacientes	275
transcription polymerase	275
standard deviation	275
mrna levels	275
different types	274
cfu ml	274
two cases	274
plasma samples	273
multivariate analysis	273
bronchoalveolar lavage	272
genome sequence	272
positive cells	272
copies per	271
lateral flow	271
united kingdom	270
antibiotic resistance	270
within days	269
cell count	269
west nile	269
findings suggest	269
infection control	269
analytical sensitivity	268
ex vivo	268
human rhinovirus	268
syndrome virus	267
age groups	267
current study	267
respiratory distress	266
swab samples	266
diagnostic accuracy	266
also found	265
diagnostic test	265
statistical significance	265
even though	265
may contribute	265
herpes simplex	264
negative controls	264
positive result	264
clinical manifestations	264
pseudomonas aeruginosa	263
viral respiratory	262
weight loss	261
clinical course	261
standard curve	261
transplant recipients	261
viral shedding	259
clinical microbiology	259
confirmed cases	259
immunodeficiency virus	259
western blotting	257
molecular diagnostics	257
also observed	257
platelet concentrates	256
severe disease	256
may lead	256
acid sequence	256
study group	255
cancer cell	255
human respiratory	255
rna polymerase	255
serial dilutions	254
per reaction	254
allogeneic stem	254
diabetes mellitus	254
primer pair	254
medrxiv preprint	254
blood culture	253
mortality rate	253
molecular detection	253
transcription factor	253
bacterial infections	253
among patients	253
viral culture	253
heart failure	253
nitric oxide	252
viral nucleic	251
positive rt	251
cd cell	250
virus type	250
developing countries	250
blot analysis	250
fever virus	249
class i	249
inflammatory cytokines	249
nucleotide sequence	248
age group	248
data analysis	248
clostridium difficile	248
multiple myeloma	248
dependent manner	248
virus replication	247
multiplex rt	247
rapid diagnosis	247
disease progression	247
primer sets	246
negative rt	246
dna amplification	246
clinical trial	246
nasal swabs	246
molecular biology	245
immunocompromised patients	245
transfusion reactions	245
patients infected	244
authors declare	244
liver disease	244
pcr reactions	243
regression analysis	243
several studies	242
using real	241
cell lymphoma	241
pcr primers	241
high throughput	241
may play	240
international license	240
ct scan	239
antibiotic therapy	239
quantitative rt	239
early diagnosis	239
pulmonary disease	239
four patients	238
patient samples	238
drug resistance	238
sars coronavirus	238
dna extraction	237
ml min	236
highly conserved	236
differentially expressed	236
nile virus	236
spike protein	235
high level	235
reactive protein	235
study showed	235
coronavirus infection	235
cell transplant	235
positive cases	233
taken together	233
antigen detection	232
mycoplasma pneumoniae	232
conventional pcr	231
last years	231
digital pcr	231
significantly decreased	230
target dna	229
molecular mechanisms	229
viral particles	229
blood stem	228
stranded dna	228
susceptibility testing	228
dna sequencing	228
fold increase	227
clinically significant	227
well tolerated	226
negative samples	226
graft versus	226
genetic diversity	226
ct findings	224
complete genome	224
samples tested	224
tissue culture	224
se recomienda	224
computed tomography	224
medical records	223
unrelated donor	223
next generation	223
en los	223
reverse primer	222
detection system	222
east respiratory	221
old male	221
ct value	221
causative agent	220
molecular diagnosis	220
may provide	220
old female	220
one year	219
primer set	219
clinically relevant	219
per year	219
laboratory diagnosis	218
target genes	218
analysis using	218
new york	218
data indicate	217
using pcr	217
enteric viruses	217
transgenic mice	217
may occur	217
swine fever	217
within hours	216
gestational age	216
single nucleotide	216
rights reserved	216
five patients	216
confidence interval	215
allogeneic hsct	215
early detection	215
bacterial infection	215
target sequence	215
blood banks	214
positive controls	214
critically ill	214
simplex virus	213
age years	213
nucleotide sequences	213
gastrointestinal tract	212
sensitive detection	212
stranded rna	212
feline coronavirus	212
high dose	211
diagnostic testing	211
retrospective analysis	211
rna virus	210
genbank accession	210
protein levels	210
pathogen detection	209
copy numbers	209
transfusion service	209
preliminary results	209
graft failure	209
viral dna	208
antibody response	208
sanger sequencing	208
classical swine	208
bacterial pathogens	208
curve analysis	208
small number	207
versus host	207
hospitalized children	207
adverse effects	207
molecular characterization	206
positive rate	206
cumulative incidence	206
canine parvovirus	206
myeloid leukemia	205
extracellular matrix	205
time polymerase	205
chlamydia pneumoniae	204
results demonstrate	204
serum levels	204
hepatocellular carcinoma	204
blood collection	204
blood donations	204
previous study	204
con covid	204
antibiotic treatment	203
two weeks	202
low levels	202
sequence data	202
drinking water	201
physical examination	201
renal disease	201
tested using	200
patients showed	200
cell activation	200
squamous cell	200
tested negative	200
genome sequencing	200
acid detection	199
transport medium	199
study population	199
faecal samples	199
based assays	199
based detection	199
antibody responses	199
time point	198
mini kit	198
free water	197
human coronaviruses	197
also used	197
kidney disease	197
blood supply	196
reaction mixture	196
antimicrobial resistance	196
assessed using	196
significant reduction	196
laboratory tests	196
based methods	196
diagnostic tool	196
molecular diagnostic	195
clinical outcome	195
general population	195
cmv reactivation	195
high specificity	195
bovine serum	195
pcr methods	195
highly pathogenic	195
conclusions reached	194
autologous stem	194
three times	194
authority reserves	194
issues addressed	194
sample size	193
nephrotic syndrome	193
output adopted	193
may cause	193
awarded following	193
colorectal cancer	193
transparency principle	193
also detected	193
recent study	193
published complying	193
tender procedure	193
lung tissue	192
inclusion criteria	192
healthy donors	192
igg antibodies	191
sputum samples	191
cdna synthesis	191
calculated using	191
throat swabs	191
multiple sclerosis	191
north america	191
human immunodeficiency	191
specific pcr	190
may result	190
acute myeloid	189
turnaround time	189
one hundred	189
study aimed	189
deficient mice	188
healthy dogs	188
porcine reproductive	188
control subjects	187
significant correlation	187
resistance genes	187
cell responses	187
small intestine	187
evaluated using	187
future studies	187
plasmid dna	187
blood units	186
immune cells	186
expression level	186
host cell	186
without prejudice	186
emergency department	186
healthcare workers	186
bodies identified	185
currently available	185
molecular testing	185
vero cells	184
patients developed	184
genome sequences	184
naturally occurring	184
incubation period	183
cell viability	183
positive pcr	183
viral diseases	183
sequence alignment	183
low viral	183
year period	183
grade ii	182
family members	182
early stage	182
assay using	182
national institute	182
parainfluenza viruses	182
gene sequences	181
clinical outcomes	181
per day	181
white blood	181
blood center	180
positive correlation	180
experimentally infected	180
myeloablative conditioning	180
like particles	180
molecular epidemiology	180
viral detection	180
control groups	179
strains isolated	179
exact test	179
old woman	178
nested rt	178
realtime pcr	178
sore throat	178
canine distemper	178
molecular assays	178
control measures	177
animal models	177
rrna gene	177
lamp assay	177
data show	177
consecutive patients	177
also showed	177
factors associated	176
clinical laboratory	176
marrow transplantation	175
specific antibody	175
test result	175
clinical disease	175
chronic obstructive	175
study included	175
positive test	174
significant decrease	174
nasopharyngeal aspirates	174
may represent	174
reference standard	174
epithelial cell	174
circovirus type	174
similar results	174
barr virus	173
antimicrobial susceptibility	173
group i	173
ill patients	173
first step	173
growth factors	172
infected individuals	172
transmission electron	172
common cold	172
swab specimens	171
see table	171
three groups	171
relatively high	171
control study	171
virus rna	170
detected using	170
virus strains	170
transmissible gastroenteritis	170
grade iii	170
positive specimens	170
may help	170
one study	170
life technologies	170
pilot study	170
respiratory failure	169
clinical studies	169
laboratory testing	169
ethics committee	169
open reading	169
acute phase	168
influenza vaccine	168
samples using	168
tract disease	168
linear regression	168
rapid identification	168
whole genome	168
abdominal pain	168
obstructive pulmonary	168
serological tests	168
amplification assay	168
broad spectrum	168
thermal cycler	167
higher risk	167
six patients	167
transcription factors	167
molecular techniques	167
viral genomes	167
based assay	167
clinical laboratories	167
samples obtained	167
related mortality	167
relatively low	166
fold higher	166
new zealand	166
also tested	165
mechanical ventilation	165
culture medium	165
signal transduction	165
gene therapy	165
major cause	165
structural proteins	165
high mortality	164
final extension	164
resistant strains	164
per patient	164
univariate analysis	164
blood flow	163
times higher	163
transcriptase polymerase	163
clinical significance	163
cell counts	163
nk cell	163
swine influenza	163
forward primer	163
two years	163
newcastle disease	163
antibody titers	163
confirmed covid	163
higher sensitivity	162
significant association	162
higher levels	162
vaccine strain	162
cytopathic effect	162
specific detection	162
binding sites	162
patients died	162
highly specific	162
culture media	162
urine samples	161
high viral	161
hospital admission	160
previously published	160
direct contact	160
well known	160
another study	160
plant mirnas	160
cytokine production	160
human influenza	160
binding protein	159
factor viii	159
among children	159
using different	159
phylogenetic tree	159
green i	159
blood product	159
least two	159
one sample	159
false positives	159
reduced intensity	159
months post	159
response rate	158
host cells	158
hcv infection	158
human adenovirus	158
first report	158
blood mononuclear	158
healthy volunteers	158
dna damage	158
high frequency	158
hbv infection	158
fungal infections	157
like illness	157
bacterial contamination	157
amplification products	156
heart rate	156
distress syndrome	156
thermal cycling	156
viral transport	156
umbilical cord	156
poor prognosis	156
three cases	156
based amplification	156
mesenchymal stem	156
serum creatinine	156
also known	156
risk assessment	156
detection rates	156
hepatitis virus	155
para el	155
platelet counts	155
patients underwent	155
copies reaction	155
canine coronavirus	155
better understanding	155
using primers	155
increased expression	155
antibody test	155
also performed	155
using two	155
conventional methods	155
blood safety	155
mast cells	154
heart disease	154
fully automated	154
disease caused	154
serum albumin	154
signaling pathways	154
genomic rna	154
blood loss	153
general hospital	153
reactive oxygen	153
rna extracted	153
pcr result	152
transcriptase pcr	152
thermo fisher	152
donor chimerism	152
day post	152
naturally infected	152
target gene	152
negative predictive	152
diagnostic tools	151
detection method	151
obtained using	151
hematopoietic cell	151
necrosis factor	151
mdck cells	151
multiplex reverse	150
recombinase polymerase	150
virus particles	150
throat swab	150
mycobacterium tuberculosis	150
supplementary table	150
specific primer	150
patient care	150
leading cause	150
distemper virus	150
using rt	150
experimental infection	150
suspected cases	150
polymerase amplification	149
patients admitted	149
randomly selected	149
four different	149
amplified using	149
cell type	149
body mass	149
matched unrelated	148
healthy cats	148
protein kinase	148
class ii	148
studies showed	148
first case	148
human serum	148
healthy individuals	148
suspected covid	148
animal health	147
infectious virus	147
purified using	147
design methods	147
cd expression	147
key role	147
derived evs	147
viral pneumonia	147
intensity conditioning	147
pcr amplicons	147
laser desorption	147
old man	146
eight patients	146
much higher	146
cells expressing	146
leukemia virus	146
antibody detection	146
signaling pathway	146
limited number	146
oxygen species	145
birth weight	145
virus culture	145
high incidence	145
results findings	145
expression analysis	145
primers used	145
seven patients	145
rabies virus	144
extracted using	144
replacement therapy	144
soft tissue	144
large numbers	144
infected mice	144
conditioning regimens	144
con el	144
total protein	144
antimicrobial agents	144
genetic analysis	144
nanoparticle tracking	144
buffered saline	144
rapid diagnostic	144
single cell	144
ethidium bromide	144
coronavirus infections	143
immunofluorescence assay	143
severe respiratory	143
normal range	143
reverse primers	143
coronavirus hku	143
patients presented	143
tissue sections	142
group ii	142
analysed using	142
time period	142
showed significant	142
virus isolates	142
capillary electrophoresis	142
tcid ml	141
water samples	141
animal model	141
genes involved	141
fisher scientific	141
accession number	141
crucial role	141
median number	141
observational study	141
binding site	141
human plasma	141
rna copies	141
amplifi cation	140
negative patients	140
blood vessels	140
serially diluted	140
tracking analysis	140
acute leukemia	140
cycling conditions	140
common respiratory	140
preliminary data	140
melting curve	140
done using	140
protein gene	140
medical university	139
based real	139
el uso	139
statistical analyses	139
diagnostic assays	139
patients aged	139
dna sequence	139
respiratory diseases	139
patients may	139
endothelial cell	139
transcription pcr	139
antibiotic use	139
previously shown	138
tritrichomonas foetus	138
lung injury	138
progenitor cells	138
children aged	138
reaction assay	137
nine patients	137
severe covid	137
controlled trial	137
immunohistochemical staining	137
final concentration	137
extraction kit	137
vaccine strains	137
muscle cells	137
positive blood	137
quantitative detection	137
proinflammatory cytokines	137
positive predictive	136
acid sequences	136
data collection	136
epidemiological studies	136
early stages	136
plasma cells	136
stromal cells	136
rrt pcr	136
zika virus	136
th day	136
ovarian cancer	135
university medical	135
effective treatment	135
included patients	135
pulmonary function	135
hospital stay	135
human rhinoviruses	135
first days	135
cell receptor	135
genes encoding	135
negative bacteria	135
studies using	135
hematological malignancies	135
study shows	134
fatty acid	134
orf ab	134
month period	134
left ventricular	134
disease status	134
antiviral therapy	134
emerging infectious	134
igm antibodies	134
airway epithelial	134
years ago	133
cells showed	133
symptomatic patients	133
infected cats	133
insulin resistance	133
mixed infections	133
converting enzyme	133
dna samples	133
van een	133
patients diagnosed	133
veterinary medicine	133
per sample	133
poorly understood	132
enzyme immunoassay	132
rbc units	132
older adults	132
reference gene	132
ms ms	131
pfu ml	131
adenovirus type	131
blood services	131
foster city	131
fatty acids	131
better understand	131
fusion protein	131
igg antibody	131
first study	131
odds ratio	131
treated cells	131
liver biopsy	131
protein level	131
sickle cell	131
nasal swab	130
aged years	130
ca i	130
transcription loop	130
results indicated	130
helicobacter pylori	130
well established	130
mouth disease	130
healthy subjects	130
case series	130
skeletal muscle	130
gene encoding	130
neutralizing antibodies	130
autologous blood	130
specific cd	130
new coronavirus	129
low level	129
transplant patients	129
care testing	129
higher prevalence	129
first year	129
dna sequences	129
cell growth	129
methods used	128
ebola virus	128
cell source	128
transfusion services	128
antigen test	128
dna microarray	128
exome sequencing	128
high resolution	128
clinical management	128
dna viruses	128
south korea	128
survival rate	127
institutional review	127
hiv infection	127
adverse reactions	127
pcr master	127
cycle threshold	127
data obtained	127
prognostic factors	127
bovine respiratory	127
months later	127
innate immunity	127
tumor necrosis	127
clinical use	126
liquid chromatography	126
remains unclear	126
european countries	126
common cause	126
high affinity	126
gene sequence	126
roche diagnostics	126
cells using	126
control samples	126
patients presenting	126
method using	126
tumour cells	126
previous reports	126
showed high	126
patient management	125
conserved regions	125
accession numbers	125
low dose	125
del paciente	125
clinical evaluation	125
nucleocapsid protein	125
fresh frozen	125
primer design	125
family history	125
south africa	125
bovine coronavirus	125
written informed	125
inflammatory responses	125
reference laboratory	125
high degree	125
gold nanoparticles	125
review board	125
probe sets	124
using specific	124
cardiovascular disease	124
gastroenteritis virus	124
primer probe	124
well plates	124
severe cases	124
inflammatory bowel	124
randomized controlled	124
will help	124
viral antigen	124
plasma levels	124
detection limits	124
past years	124
healthy children	124
direct detection	124
gene mutations	124
acid testing	123
correlation coefficient	123
detection kit	123
frozen plasma	123
unrelated donors	123
significant changes	123
regression model	123
mortality rates	123
patient died	123
relapse mortality	123
reuse allowed	123
inhibitory effect	123
potential role	123
bronchial epithelial	123
specimens collected	123
infectious complications	123
deep sequencing	123
colon cancer	123
surface expression	123
antibody tests	123
investigate whether	123
viral sequences	123
viruses using	123
acute renal	123
cell adhesion	123
without permission	123
allowed without	123
qpcr assay	123
blood component	122
renal biopsy	122
national health	122
accurate diagnosis	122
microscopic examination	122
children hospitalized	122
nucleotide polymorphisms	122
critical role	122
median duration	122
final volume	122
flow cytometric	122
tyrosine kinase	122
autosomal recessive	122
acute lymphoblastic	122
hodgkin lymphoma	121
definitive diagnosis	121
autoimmune diseases	121
rapid antigen	121
type diabetes	121
pcr based	121
process control	121
virus strain	121
study demonstrates	121
mouse hepatitis	121
pcr negative	121
coronavirus pneumonia	121
reading frame	121
mass index	121
fragment length	121
clinical relevance	121
reference strains	121
restriction enzyme	121
amniotic fluid	121
wound healing	120
diagnostic performance	120
rna detection	120
human papillomavirus	120
ct scans	120
alkaline phosphatase	120
low sensitivity	120
medical history	120
magnetic beads	120
throughput sequencing	120
primer sequences	120
otitis media	120
compared using	120
rna isolation	120
infl uenza	120
dna fragments	119
quality assurance	119
patient population	119
useful tool	119
desorption ionization	119
antibody screening	119
body fluids	119
klebsiella pneumoniae	119
red cross	119
negative result	118
donor selection	118
using standard	118
infections caused	118
blood volume	118
candidate genes	118
less sensitive	118
will provide	118
supplementary material	118
nanotrap particles	118
enzyme activity	118
two methods	118
performed according	118
high rate	118
competing interests	118
sequence identity	118
single center	118
clinical setting	117
dose chemotherapy	117
frequently used	117
one week	117
conducted using	117
combination therapy	117
cell dose	117
patient received	117
antimicrobial therapy	117
lymphoblastic leukemia	117
amplified products	117
adipose tissue	117
direct sequencing	117
molecular analysis	117
data demonstrate	116
protective equipment	116
cells may	116
per ml	116
sin embargo	116
frequently detected	116
acute gastroenteritis	116
blood sample	116
samples showed	116
national blood	116
sequencing data	116
chronic inflammation	116
increased levels	115
underlying disease	115
bowel disease	115
glial cells	115
laboratory mice	115
abi prism	115
extraction methods	115
three months	115
amplification method	115
specific rt	115
cd ra	115
diagnostic sensitivity	115
avian infectious	115
por covid	115
inflammatory cells	115
time course	115
penaeus monodon	115
large scale	114
like receptor	114
considered significant	114
lavage fluid	114
chronic renal	114
coding region	114
cd positive	114
dna methylation	114
square test	114
elderly patients	114
considered positive	114
risk patients	114
teaching hospital	114
detection using	114
bl mice	114
increased significantly	113
sensitive method	113
treated patients	113
mouse models	113
endoplasmic reticulum	113
amplification efficiency	113
research institute	113
may affect	113
antiviral drugs	113
possible role	113
retrospectively analyzed	113
additional file	113
lysis buffer	113
antiglobulin test	113
patient developed	113
literature review	113
virus dna	113
molecular tests	113
target cells	113
cmv infection	113
false negatives	113
patients suffering	112
blood service	112
septic shock	112
target sequences	112
viruses detected	112
cell membrane	112
age range	112
will allow	112
platelet engraftment	112
vitro activity	112
antiviral treatment	112
identified using	112
confocal microscopy	112
taqman probe	112
acute graft	112
laboratory data	112
housekeeping genes	112
mhc class	112
six months	112
also included	112
grade i	112
will also	111
recent advances	111
different time	111
brain tissue	111
mg day	111
reaction volume	111
reverse transcribed	111
high grade	111
lactate dehydrogenase	111
year os	111
infected animals	111
time consuming	111
standard curves	111
assisted laser	111
two major	111
older patients	111
wide variety	111
viral etiology	111
one step	111
en un	110
immunosuppressive therapy	110
diagnostic laboratories	110
encephalitis virus	110
receptor binding	110
drug administration	110
diagnostic techniques	110
first line	110
complete response	110
different methods	110
se ha	110
economic losses	110
specimen collection	110
viral antigens	110
days later	110
czech republic	110
new england	109
blood centers	109
patients compared	109
coronavirus nl	109
culture supernatants	109
diagnostic value	109
study suggests	109
knockout mice	109
microarray analysis	109
domestic cats	109
platelet transfusion	109
respiratory coronavirus	109
primary immunodeficiency	109
serological testing	109
human health	109
uric acid	109
fluorescence intensity	109
examination revealed	109
ko mice	109
immune reconstitution	109
personal protective	108
vascular endothelial	108
consecutive days	108
environmental samples	108
tumor cell	108
pcr inhibitors	108
mycophenolate mofetil	108
reported cases	108
different concentrations	108
rna molecules	108
liver cirrhosis	108
antibody testing	108
cell function	108
log copies	108
length polymorphism	108
melting temperature	108
corona virus	108
total nucleic	108
group compared	108
microbiology laboratory	108
low risk	108
yellow head	108
coronavirus associated	108
clinical findings	107
pathogenic avian	107
one month	107
histological examination	107
kg body	107
north american	107
cell therapy	107
magnetic resonance	107
multiple sequence	107
short time	107
infection may	107
takes place	107
exogenous ncrnas	107
dynamic range	107
human herpesvirus	107
serologic testing	107
human genome	107
pcr primer	107
transport media	107
secondary structure	107
different species	106
duplex real	106
ml kg	106
clinical information	106
neutrophil engraftment	106
asthma exacerbation	106
aml patients	106
extremely rare	106
many patients	106
rbc transfusion	106
asthma exacerbations	106
may reflect	106
expression profiles	106
aplastic anemia	106
respiratory pathogen	106
full length	106
diagnostic laboratory	106
light microscopy	106
increasing number	106
three days	106
total volume	106
buffy coat	106
first two	106
natural killer	106
scoring system	106
positive sample	105
oropharyngeal swabs	105
fungal infection	105
many cases	105
cell differentiation	105
confidence intervals	105
inflammatory cytokine	105
laboratory findings	105
rna sequencing	105
higher incidence	105
virus genome	105
el paciente	105
major role	105
culture supernatant	105
antigen tests	105
two samples	105
dilution series	105
treatment group	105
expression pattern	105
phylogenetic trees	105
extracted rna	105
clinical sensitivity	105
differential expression	105
virus transmission	105
different cell	105
taq dna	105
life cycle	105
bovine viral	105
tertiary care	104
acute infection	104
many studies	104
control cells	104
signalling pathways	104
retrospective cohort	104
probably due	104
porcine respiratory	104
gi tract	104
protective effect	104
vast majority	104
close contact	104
sars patients	104
samples taken	104
influenza infection	104
likely due	104
th percentile	104
isolated using	103
many countries	103
well plate	103
serological assays	103
wt mice	103
laboratory animal	103
cov infection	103
allowed us	103
survival rates	103
paraffin embedded	103
bacterial species	103
skin lesions	103
haemophilus influenzae	103
amplification reaction	103
vitro studies	103
using flow	103
dna microarrays	102
immunohistochemical analysis	102
many different	102
small cell	102
san diego	102
raw sewage	102
cells infected	102
blood count	102
samples positive	102
convalescent plasma	102
liver injury	102
two days	102
significantly correlated	102
host immune	102
por el	102
rna genome	102
microglial cells	102
genome copies	102
invasive fungal	102
covid pos	102
studies suggest	101
blood groups	101
membrane protein	101
nkt cells	101
rna samples	101
pcr conditions	101
antiviral activity	101
haematopoietic stem	101
mammalian cells	101
liver fibrosis	101
two distinct	101
clinical sample	101
resistant staphylococcus	101
also reported	101
important cause	101
consensus sequence	101
persistent infection	101
breast milk	101
reaction mix	101
initial denaturation	101
pet birds	101
studies will	101
specific dna	101
matched sibling	101
fold dilutions	100
influenza pandemic	100
brain barrier	100
body temperature	100
experimental conditions	100
annealing temperature	100
biological samples	100
inflammatory diseases	100
table shows	100
study using	100
may explain	100
infection among	100
dna rna	100
nucleotide identity	100
renal transplantation	100
global health	100
recently described	100
biopsy specimens	100
post transplant	100
genetic material	100
stool specimens	100
small rna	100
van het	100
human disease	100
infect dis	100
samples containing	100
hemolytic anemia	100
may serve	100
gene segments	99
mitral valve	99
expression patterns	99
ms phage	99
coronavirus oc	99
diagnostic criteria	99
healthy control	99
green real	99
acute lower	99
two viruses	99
cardiac surgery	99
genetic variation	99
broad range	99
chronic kidney	99
high quality	99
assays using	99
capsid protein	99
routine use	99
marrow transplant	99
study also	99
viral proteins	99
species identification	99
protein concentration	99
low grade	99
cultured cells	98
pulmonary fibrosis	98
average age	98
threshold cycle	98
single dose	98
quantitative reverse	98
taq polymerase	98
severe pneumonia	98
infection rate	98
white matter	98
culture system	98
among others	98
weight gain	98
highly expressed	98
cell disease	98
airway inflammation	98
pathogenic bacteria	98
gene rrt	98
phase i	98
quantitative analysis	98
financial support	98
liver tissue	98
strand displacement	98
purification kit	98
young adults	98
significant effect	98
saudi arabia	97
low cost	97
cell wall	97
human infection	97
internal controls	97
influenza surveillance	97
liver transplantation	97
also significantly	97
case reports	97
hela cells	97
care workers	97
vertical transmission	97
oral administration	97
investigated whether	97
screening test	97
clinical performance	97
chronic inflammatory	97
bij een	97
feline leukemia	97
newly diagnosed	96
significantly improved	96
restriction fragment	96
rna mini	96
infected cell	96
vp gene	96
influenza season	96
significant improvement	96
significantly less	96
clinical impact	96
virus types	96
sars cov	96
national university	96
highly contagious	96
generated using	96
newly discovered	96
respiratory secretions	96
tract samples	96
old boy	96
ct imaging	96
detect sars	95
blood glucose	95
cells kg	95
mm tris	95
rheumatoid arthritis	95
university school	95
one day	95
dependent rna	95
superscript iii	95
health problem	95
patients hospitalized	95
mean value	95
multiplex assays	95
platelet aggregation	95
contact tracing	95
progressive disease	95
pcr protocol	95
detection assay	95
fluid samples	94
present work	94
liver function	94
met een	94
lupus erythematosus	94
neoplastic cells	94
published data	94
amplification methods	94
glomerular filtration	94
viruses isolated	94
trend towards	94
sectional study	94
also evaluated	94
genetic variants	94
stage renal	94
human cells	94
quantified using	94
partial response	94
mast cell	94
breast carcinoma	94
expressed genes	94
risk group	94
remains unknown	94
different regions	94
van der	94
activated protein	94
asymptomatic patients	94
exclusion criteria	93
bp fragment	93
retrospective review	93
mcg ml	93
year overall	93
hek cells	93
study provides	93
developed countries	93
point mutations	93
routine laboratory	93
five years	93
may influence	93
three years	93
head virus	93
cell migration	93
hours post	93
also identified	93
patients transplanted	93
viral isolation	93
much lower	93
different stages	93
nasopharyngeal aspirate	93
viral pathogen	93
patients whose	93
pneumocystis jirovecii	93
mm mgcl	92
quality assessment	92
within min	92
surface antigen	92
exclusion chromatography	92
toxoplasma gondii	92
coat protein	92
findings indicate	92
prognostic factor	92
viral disease	92
study aims	92
provide evidence	92
bacillus anthracis	92
conserved region	92
human parainfluenza	92
significantly greater	92
platelet activation	92
weeks gestation	92
information regarding	92
negative correlation	92
posted june	92
low prevalence	92
treated mice	92
species level	92
iron deficiency	92
gene amplification	92
heat shock	92
data collected	92
cell populations	92
immunodeficient mice	92
binding proteins	92
elevated levels	92
phylogenetic analyses	92
potential risk	92
fold serial	92
amplification tests	91
routine clinical	91
diffuse large	91
multicenter study	91
recipient cells	91
two consecutive	91
survival time	91
hubei province	91
viral agents	91
dna levels	91
early phase	91
cell lysates	91
chlamydia trachomatis	91
antigen expression	91
natural history	91
using multiplex	91
amplification techniques	91
coronavirus rna	91
type mice	91
differ significantly	91
twice daily	91
four cases	91
reverse genetics	91
expression profile	91
based pcr	91
surveillance system	91
inflammatory mediators	91
japanese encephalitis	90
negative test	90
polymerase gene	90
cell subsets	90
bal fluid	90
two independent	90
first months	90
first reported	90
recently developed	90
two strains	90
surface area	90
solid organ	90
antibiotic susceptibility	90
hematologic malignancies	90
complete blood	90
sibling donor	90
recombinant proteins	90
myocardial infarction	90
virulence factors	90
descriptive study	90
function tests	90
fluorescence signal	90
results may	90
liver failure	90
mechanisms underlying	90
disk diffusion	89
ct features	89
cycle sequencing	89
currently used	89
colloidal gold	89
disease free	89
prospective studies	89
pcr technique	89
cell lung	89
time detection	89
data suggests	89
internal medicine	89
monoclonal anti	89
qiaamp viral	89
pleural effusion	89
rsv infection	89
density gradient	89
comparative analysis	89
least days	89
cleavage site	89
respiratory illnesses	89
among different	89
dna detection	89
bacterial growth	89
laboratory test	89
rna template	89
usa introduction	89
significant role	89
extraction method	89
often used	88
external quality	88
also increased	88
human cases	88
may develop	88
study revealed	88
study conducted	88
merkel cell	88
clinical suspicion	88
like receptors	88
showed similar	88
matched related	88
dis doi	88
nosocomial infections	88
ms plp	88
diagnostic method	88
bacterial culture	88
binding domain	88
genbank database	88
gene mutation	88
two types	88
highly variable	88
hsct recipients	88
blood transfusions	88
first trimester	88
mean number	88
size distribution	88
total body	88
icu patients	88
hemorrhagic fever	88
central role	88
ha gene	88
pulmonary hypertension	87
found positive	87
patient outcomes	87
control dogs	87
scid mice	87
second round	87
cell population	87
library preparation	87
performance characteristics	87
medical record	87
three samples	87
using either	87
molecular evolutionary	87
partial remission	87
influenza vaccination	87
human adenoviruses	87
chronic liver	87
environmental conditions	87
nasopharyngeal samples	87
signal amplification	87
associated coronavirus	87
dna template	87
primary care	87
environmental factors	87
lymphocyte count	87
fatality rate	87
expression profiling	87
flight mass	87
also shown	87
community acquired	87
female patients	87
new cases	87
primary human	87
th cells	87
using primer	86
wistar rats	86
clinical settings	86
chikungunya virus	86
clinical applications	86
assay results	86
genomic sequence	86
en las	86
detection assays	86
fluorescence microscopy	86
urinary bladder	86
different clinical	86
low molecular	86
test kit	86
male patients	86
hla class	86
fold change	86
oral cavity	86
autoimmune disease	86
high expression	86
endemic areas	86
treatment option	86
plant viruses	86
health status	86
interest reported	86
nutritional status	86
antibody screen	86
transfusion practice	86
adaptive immune	86
virus using	86
high concentrations	86
transfusion therapy	86
cell carcinomas	86
specific probes	86
long time	86
serum concentrations	86
urgent need	85
negative pcr	85
new therapeutic	85
also present	85
also demonstrated	85
virus load	85
high number	85
induced apoptosis	85
multiplex assay	85
myelodysplastic syndrome	85
image analysis	85
high fat	85
key words	85
female donors	85
fluorescent signal	85
acute asthma	85
significant impact	85
extracellular vesicle	85
also positive	85
novel human	85
true positive	85
pcr positivity	85
white spot	85
per unit	85
evidence suggests	85
first week	85
dna copies	85
data showed	84
respiratory panel	84
died due	84
human dna	84
pathogen identification	84
protein synthesis	84
nanopore sequencing	84
indirect immunofluorescence	84
expression studies	84
treatment groups	84
related complications	84
live attenuated	84
molecular beacons	84
molecular typing	84
organ failure	84
sample volume	84
apoptotic cells	84
england biolabs	84
viral hepatitis	84
flow rate	84
mean values	84
within months	84
tract specimens	84
agar dilution	84
old girl	84
resonance imaging	84
cell depletion	83
los casos	83
plaque assay	83
cd count	83
last decade	83
immune cell	83
haploidentical donor	83
con un	83
significantly elevated	83
realtime rt	83
data support	83
like virus	83
pcr techniques	83
recently reported	83
several days	83
virus shedding	83
cause severe	83
term follow	83
mm patients	83
previously identified	83
mixed chimerism	83
time interval	83
recombinant protein	83
disease onset	83
positive bacteria	83
rank test	83
glass opacities	83
specific amplification	83
lamina propria	83
nasal discharge	83
human cytomegalovirus	83
residual disease	83
con una	83
first round	83
rna interference	82
sexually transmitted	82
potential therapeutic	82
hcv genotype	82
may reduce	82
using high	82
statistical difference	82
neurological signs	82
con sospecha	82
medical research	82
i ii	82
wild birds	82
national center	82
hiv positive	82
turkey objective	82
different countries	82
stage iii	82
cells per	82
based method	82
pivotal role	82
outer membrane	82
also investigated	82
chicken eggs	82
virus detected	82
immune deficiency	82
positivity rate	82
schwann cells	82
muscle actin	82
cell number	82
positive sars	82
agarose gels	82
medical staff	82
gram negative	82
particle size	82
designed using	82
chronic graft	82
reporter gene	82
standard methods	82
results demonstrated	82
salivary gland	82
taqman rt	81
group system	81
new method	81
paciente con	81
small rnas	81
fat diet	81
rna sequences	81
high morbidity	81
small sample	81
method used	81
within minutes	81
successfully treated	81
pcr buffer	81
causative agents	81
step real	81
based tests	81
relapse rate	81
case definition	81
one hour	81
using molecular	81
medical school	81
showed higher	81
body condition	81
way anova	81
human lung	81
south america	81
qpcr assays	81
fecal specimens	81
systemic lupus	81
gene transcription	81
oxygen saturation	81
reference sequence	81
infectious agent	81
low concentrations	81
mixed infection	81
biological activity	81
per week	81
pneumoniae infection	81
infected dogs	81
bacterial pneumonia	81
time donors	80
adhesion molecules	80
clinical application	80
clinical response	80
probe sequences	80
mosaic virus	80
therapeutic strategies	80
examination showed	80
distilled water	80
tissue specimens	80
mesenteric lymph	80
water bath	80
decision making	80
rapid test	80
microbiology laboratories	80
hospital admissions	80
using three	80
magna pure	80
membrane potential	80
viral diarrhea	80
influenza vaccines	80
study demonstrated	80
different viruses	80
filtration rate	80
promoter activity	80
also associated	80
neutralizing antibody	80
male patient	80
tumor size	80
clinical study	80
salmonella spp	80
antibody production	80
human airway	80
cells ml	80
patient groups	80
household contacts	80
showed significantly	80
less likely	80
venous blood	80
control mice	79
droplet digital	79
enveloped viruses	79
donate blood	79
surface markers	79
female patient	79
ribosomal rna	79
newly developed	79
pasteurella multocida	79
kans op	79
arachidonic acid	79
evolutionary genetics	79
energy transfer	79
five different	79
mean duration	79
se han	79
host range	79
hydrogen peroxide	79
maximum likelihood	79
significantly better	79
adhesion molecule	79
dose dependent	79
organ transplantation	79
acute lung	79
final diagnosis	79
sample types	79
clostridium perfringens	79
control patients	79
platelet function	79
technical assistance	79
field samples	79
pcr systems	79
human pathogens	79
total dna	79
brain injury	79
viral clearance	79
solid tumors	79
patient group	79
testing using	79
icu admission	79
cell response	78
routine diagnostic	78
sialic acid	78
main cause	78
ferritin levels	78
routinely used	78
asymptomatic carriers	78
infected pigs	78
ibv strains	78
efsa supporting	78
previously demonstrated	78
feline herpesvirus	78
resistant isolates	78
research complications	78
comparative study	78
transplanted patients	78
type iii	78
antibody levels	78
tests performed	78
russian federation	78
vaccine development	78
metapneumovirus infection	78
patient age	78
patient presented	78
viral titers	78
medium containing	78
infected children	78
index case	78
gastric cancer	78
interquartile range	78
multiple pathogens	78
fetal bovine	78
significant proportion	78
transfusion reaction	78
supporting publication	78
dna extracted	78
diagnostic yield	78
first described	78
significant number	78
genetic characterization	77
weeks later	77
using dna	77
complementary dna	77
cost effective	77
porcine apn	77
important roles	77
diagnostic assay	77
isolation kit	77
preterm birth	77
strand rna	77
bartonella spp	77
probe set	77
genomic sequences	77
clinical parameters	77
readily available	77
detect viral	77
asthma control	77
months old	77
blood banking	77
molecular basis	77
patients included	77
emergency use	77
tumor suppressor	77
kg bw	77
renal insufficiency	77
odds ratios	77
values obtained	77
animal species	77
also determined	77
solid phase	77
three weeks	77
connective tissue	77
donor screening	77
respiratory system	77
new insights	77
model system	77
therapeutic option	77
speci fi	77
showed positive	77
forming units	77
lipid peroxidation	77
exogenous rnas	77
state university	77
legionella pneumophila	77
associated pneumonia	77
line therapy	77
patients tested	77
two main	77
size exclusion	77
voluntary blood	77
viruses including	77
stimulating factor	76
normal human	76
large amounts	76
antibody titer	76
prospective cohort	76
assess whether	76
higher rates	76
testing methods	76
genetic testing	76
human blood	76
acute rejection	76
fold dilution	76
sample processing	76
animal medicine	76
derived exosomes	76
different tissues	76
specific binding	76
agar plates	76
conditioned media	76
massive transfusion	76
patient underwent	76
successfully used	76
fetal growth	76
may require	76
inflammatory markers	76
induced sputum	76
average number	76
pneumoniae isolates	76
etiologic agent	76
less frequently	76
oligonucleotide probes	76
cases showed	76
posted august	76
spot syndrome	76
will require	75
cellular uptake	75
ionization mass	75
care units	75
genetics analysis	75
disease course	75
related viruses	75
five days	75
cell survival	75
correctly identified	75
pulmonary embolism	75
fetal dna	75
days prior	75