quadgram

This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

quadgram	frequency
severe acute respiratory syndrome	149
in the supplemental material	144
s in the supplemental	108
middle east respiratory syndrome	79
acute respiratory syndrome coronavirus	79
east respiratory syndrome coronavirus	60
in the lungs of	52
in the absence of	51
table s in the	47
in the presence of	46
of severe acute respiratory	44
h n influenza virus	36
in the case of	32
in the united states	31
see table s in	29
at an moi of	28
in the context of	25
has been shown to	24
activation of rnase l	22
h n influenza a	22
cov nsp and nsp	22
of middle east respiratory	22
article may be found	21
for this article may	21
material for this article	21
supplemental material for this	21
may be found at	21
this article may be	21
n and h n	21
a h n virus	20
porcine epidemic diarrhea virus	19
at the time of	19
human immunodeficiency virus type	19
it is possible that	19
putative small rna loci	18
the severe acute respiratory	18
of nsp and nsp	18
was added to the	18
highly pathogenic avian influenza	18
n influenza a virus	17
for the presence of	17
de genes at hpi	17
a in the supplemental	17
s a in the	17
cells were infected with	17
h n and h	16
of the viroporin domain	15
on influenza virus transmission	15
on the basis of	15
the presence of the	15
the host response to	15
it is important to	15
with the exception of	15
of allergy and infectious	14
of h n influenza	14
influenza a h n	14
of influenza a virus	14
national institutes of health	14
allergy and infectious diseases	14
as well as the	13
a multiplicity of infection	13
cells were cultured in	13
similar to that of	13
of the urokinase pathway	13
national institute of allergy	13
of a novel coronavirus	13
respiratory droplets or aerosols	13
similar to those of	13
via respiratory droplets or	13
institute of allergy and	13
acute respiratory distress syndrome	13
these data suggest that	13
p x r ko	13
pathogens with pandemic potential	13
have been deposited in	12
be found at http	12
after the onset of	12
to that of the	12
at a multiplicity of	12
at the end of	12
org lookup suppl doi	12
at room temperature for	12
x r ko mice	11
to determine whether the	11
for the development of	11
of the h n	11
the national institutes of	11
it has been shown	11
fatty acid and pl	11
the onset of symptoms	11
the innate immune response	11
of the p x	11
these results indicate that	11
a novel coronavirus from	11
in vitro and in	11
in the setting of	11
human parainfluenza virus type	11
on the cell surface	11
thermo scientific catalog no	11
with fetal bovine serum	11
by the use of	10
for min at room	10
the presence of a	10
the lungs of the	10
coexpression of nsp and	10
was used as a	10
at the same time	10
were exposed to sars	10
in the present study	10
min at room temperature	10
the p x receptor	10
cultured in the presence	10
be found at https	10
cells were transfected with	10
important to note that	10
by the presence of	10
in balb c mice	10
a wide range of	10
is important to note	10
the members of the	10
vitro and in vivo	10
see materials and methods	10
c bl j mice	10
the united states and	10
an important role in	10
in contrast to the	10
supplemented with fetal bovine	10
these results suggest that	10
respiratory syndrome coronavirus in	9
observed in the lungs	9
our understanding of the	9
see the supplemental material	9
of highly pathogenic avian	9
cells were exposed to	9
with pneumonia in saudi	9
the end of the	9
from a man with	9
in table s in	9
peripheral blood mononuclear cells	9
were found to be	9
in addition to the	9
coronavirus from a man	9
isolation of a novel	9
for the first time	9
to induce dmv formation	9
the middle east respiratory	9
of the viral genome	9
of t cell activation	9
venezuelan equine encephalitis virus	9
university of north carolina	9
of onward transmission from	9
the p x r	9
droplets or aerosols between	9
immune reconstitution inflammatory syndrome	9
the formation of dmvs	9
man with pneumonia in	9
cells were treated with	9
h n influenza viruses	9
a man with pneumonia	9
to those of the	9
of rna virus evolution	9
pneumonia in saudi arabia	9
of the fatty acid	9
novel potential pandemic pathogens	9
we were able to	9
the activation of the	9
performed as previously described	9
brief exposure to culture	9
at the university of	9
acid and pl synthesis	9
on the other hand	9
novel coronavirus from a	9
influenza virus h n	9
h n virus in	9
or aerosols between ferrets	9
to further confirm the	8
mbcs reactive to the	8
disease control and prevention	8
it is difficult to	8
room temperature for min	8
of hepatitis c virus	8
the h n influenza	8
those of the pbs	8
and cultured in the	8
centers for disease control	8
it is likely that	8
stained with hematoxylin and	8
h at room temperature	8
had no effect on	8
debate and research pause	8
cells were stained with	8
the lungs of mice	8
response to virus infection	8
with hematoxylin and eosin	8
a large number of	8
the erasmus mc facility	8
the absence of the	8
in the erasmus mc	8
virus titers in the	8
in the pathogenesis of	8
the national institute of	8
supplemental material is available	8
are shown in fig	8
is consistent with the	8
the increased delivery of	8
in the middle east	8
at the level of	8
ko and wt mice	8
it is known that	8
of the immune response	8
the public health risks	8
respiratory syndrome coronavirus infection	8
hek and vero cells	8
the role of the	8
c bl mefs were	8
the s protein of	8
is available online only	8
the ebola virus glycoprotein	8
virus transmission between ferrets	8
was observed in the	8
changes in expression of	8
the lungs of sars	8
public health risks revisited	8
material is available online	8
as well as in	8
in mice infected with	8
at late times postinfection	8
mutations in nsp and	8
text s in the	8
of the university of	8
has been shown that	8
vivo and in vitro	8
probability of onward transmission	8
in vivo and in	8
influenza virus transmission between	8
the first time that	8
infected c bl j	8
a critical role in	8
animal care and use	7
most closely related to	7
c bl j and	7
tumor necrosis factor alpha	7
of human parainfluenza virus	7
and h n influenza	7
were recovered from horses	7
cells were incubated for	7
insertion of the viroporin	7
of porcine epidemic diarrhea	7
of the authors and	7
length infectious cdna of	7
clinical signs of disease	7
airborne transmission of influenza	7
number of de genes	7
the driving force for	7
been shown to be	7
innate immune response to	7
in nsp and nsp	7
the large number of	7
loci that overlapped with	7
in the ko mice	7
cd t cell response	7
for the formation of	7
of the rdrp tree	7
of the ebola virus	7
of the nsp nsp	7
of rnase l during	7
virus titers in nasal	7
studies on influenza virus	7
the lungs of c	7
at this time point	7
with those of the	7
as the primary antibody	7
weeks after the onset	7
for disease control and	7
were infected with sinv	7
care and use committee	7
membrane insertion of the	7
pathogenic avian influenza a	7
with severe acute respiratory	7
pandemic h n influenza	7
transmission via respiratory droplets	7
into hek et cells	7
was found to be	7
the fatty acid and	7
to multiple nucleoside analogues	7
into hek t cells	7
igg mbcs reactive to	7
bl j and c	7
mice infected with a	7
of mouse adenovirus type	7
play an important role	7
the virus titers in	7
for h at room	7
and middle east respiratory	7
a member of the	7
enhanced green fluorescent protein	7
play a role in	7
influenza virus a anhui	7
h n virus between	7
of the innate immune	7
to severe acute respiratory	7
genes of unknown function	7
n virus between ferrets	7
the ability of the	7
the context of the	7
were similar to those	7
are known to be	7
value represents the mean	7
students and postdoctoral fellows	7
the specificity of the	7
by northern blot analysis	7
we were unable to	7
characterization of a novel	7
supported in part by	7
these results demonstrate that	7
is not required for	7
our data suggest that	7
upper and lower respiratory	7
in the gp core	6
the reaction products were	6
mice compared to the	6
involving pathogens with pandemic	6
were stained with hematoxylin	6
transmission of h n	6
human airway epithelial cells	6
these data indicate that	6
evolution of rna viruses	6
loss and release reports	6
of rnase l in	6
cells exposed to sars	6
the transfected cells were	6
e protein in trans	6
transmission of influenza a	6
have been shown to	6
t cell activation and	6
the absence of complement	6
monitoring select agent theft	6
used in this study	6
is a functional receptor	6
in c bl mefs	6
receptor binding site ii	6
graduate students and postdoctoral	6
from the cell surface	6
virus in the future	6
with the presence of	6
standard deviation from three	6
we are grateful to	6
eptesicus fuscus skin fibroblasts	6
suppression of t cell	6
igg against the sars	6
the views of the	6
health and human services	6
the number of years	6
significantly higher than that	6
linked glycans on gp	6
cov m gene product	6
been reported to be	6
salmonella enterica serovar typhi	6
mice infected with pfu	6
each value represents the	6
of the host response	6
of the middle east	6
analyzed for the presence	6
and influenza a virus	6
of deformed wing virus	6
influenza a virus infection	6
branches of the rdrp	6
virus replication in the	6
influenza virus transmission via	6
in cells infected with	6
research on influenza virus	6
s b and c	6
rift valley fever virus	6
department of health and	6
within the gp core	6
as a function of	6
rpmi medium supplemented with	6
upstream of rna release	6
were obtained from the	6
our results suggest that	6
in the face of	6
and the cells were	6
of influenza a h	6
of the severe acute	6
work was supported by	6
we found that the	6
convalescent subjects than in	6
and release reports in	6
antibody responses to sars	6
risks and benefits of	6
during influenza virus infection	6
and pl synthesis pathways	6
the total number of	6
reports in the united	6
to be associated with	6
was used as the	6
major histocompatibility complex class	6
the binding of niv	6
rna was extracted from	6
infected p x r	6
that were differentially expressed	6
on taxonomy of viruses	6
use research of concern	6
of pandemic influenza a	6
is known to be	6
of the complement pathway	6
in response to virus	6
h n avian influenza	6
the probability of onward	6
against severe acute respiratory	6
the antigen presentation pathway	6
c bl mice were	6
k r sv mefs	6
and veroe tmprss cells	6
after influenza virus infection	6
did not affect the	6
in accordance with the	6
at the site of	6
been deposited in genbank	6
small rna loci that	6
the international committee on	6
the purinergic receptor p	6
high concentrations of ppgpp	6
nsp and nsp are	6
of the s protein	6
b in the supplemental	6
committee on taxonomy of	6
of c bl j	6
s b in the	6
medium was replaced with	6
human respiratory syncytial virus	6
cleavage of the nsp	6
induced acute lung injury	6
the basis of the	6
cells were fixed with	6
these results suggested that	6
of putative small rna	6
incubated for h at	6
the development of a	6
not seem to be	6
crystal structure of the	6
cells were seeded in	6
vero a and huh	6
the exception of the	6
as a result of	6
vero cells infected with	6
international committee on taxonomy	6
purinergic receptor p x	6
highly pathogenic h n	6
were observed in the	6
cells were incubated with	6
the case of the	6
was supported by nih	6
determined by plaque assay	6
a functional receptor for	6
to the lack of	6
epidemic of respiratory disease	6
the glycan cap and	6
s subunit of the	6
of a h n	6
from each cluster were	6
the host immune response	6
it has been reported	6
as shown in fig	6
a broad range of	6
were incubated for h	6
virus transmission via respiratory	6
the lgi network of	6
not necessarily reflect the	6
at both time points	6
henipavirus infection in cis	6
avian h n influenza	6
to the formation of	6
of health and human	6
innate and adaptive immune	6
release reports in the	6
veroe tmprss cells were	6
on research intended to	5
protection against h n	5
of fatty acid synthesis	5
these data demonstrate that	5
for a single lab	5
rbd and s subunit	5
and s in the	5
we do not know	5
of the national institutes	5
a measure of the	5
cytopathic effect of sars	5
reactivity to h n	5
pathology of severe acute	5
that the number of	5
to the s subunit	5
production of proinflammatory cytokines	5
the results of the	5
in text s in	5
the s proteins of	5
the e protein in	5
amplified by pcr using	5
phylogenetic analysis of the	5
the activation of rnase	5
protein may function as	5
the cells were infected	5
were included in the	5
the consequences of a	5
and does not necessarily	5
mice than in the	5
of avian influenza a	5
did not result in	5
incubated for min at	5
is mediated by the	5
the university of north	5
were significantly higher in	5
virus infection in mice	5
are available upon request	5
that this is a	5
peptidase is a functional	5
the expression of gp	5
immunization with piv expressing	5
institutional animal care and	5
the cells were incubated	5
cells were maintained in	5
consequences of a lab	5
c bl mefs and	5
the pandemic h n	5
but not in the	5
work has shown that	5
driving force for m	5
do not necessarily reflect	5
reverse genetics with a	5
separation was performed on	5
host response to infection	5
effect on ev infection	5
african green monkey kidney	5
in the natural host	5
for the generation of	5
this work was supported	5
required for pkr degradation	5
the rest of the	5
of small rnas in	5
to address this issue	5
small rna loci overlapped	5
the antiviral activity of	5
protease inhibitor cocktail kit	5
in order to identify	5
and cells were incubated	5
s and table s	5
pathogenic h n influenza	5
to the development of	5
were cultured in rpmi	5
of igg against the	5
the h n virus	5
a lethal dose of	5
e orf and e	5
and g ml streptomycin	5
aids research and reference	5
were performed using the	5
to provide protection against	5
one of the most	5
authors and does not	5
to the number of	5
to influenza a virus	5
functional receptor for the	5
the infectious cdna clone	5
and elapsed time for	5
influenza a virus in	5
pellets were resuspended in	5
rna was extracted using	5
the catalytic domain of	5
the authors and does	5
the icelandic horse population	5
neutral red infectious center	5
of at least identical	5
for escape of a	5
intended to create novel	5
in materials and methods	5
our data indicate that	5
for the study of	5
immunized with combo smq	5
will be required to	5
moratorium on research intended	5
of the virus to	5
cells were washed with	5
of streptococcus equi subsp	5
novel coronavirus associated with	5
the mouse reference genome	5
of h n viruses	5
the ucsc genome browser	5
cells were washed and	5
for the emerging human	5
expression in response to	5
for min and then	5
and the number of	5
infection from his laboratory	5
as a measure of	5
was performed to detect	5
small rna libraries from	5
view of the network	5
cov and influenza virus	5
total pkr mrna levels	5
a reverse genetics system	5
cells were allowed to	5
reportedly active against sars	5
in a mouse model	5
the result is representative	5
was performed as previously	5
reflect the views of	5
of small rnas from	5
the m gene product	5
in the integral membrane	5
primary human airway epithelial	5
necessarily reflect the views	5
is shown in fig	5
at each time point	5
during the course of	5
calculation of risk and	5
h n h n	5
mg kg of body	5
of the complement system	5
the rbd and s	5
of influenza virus infection	5
resistance to multiple nucleoside	5
min hold at b	5
veroe cells were exposed	5
of a potential pandemic	5
the disease among humans	5
recombinant parainfluenza virus expressing	5
and adaptive immune responses	5
our results indicate that	5
the majority of the	5
to create novel potential	5
ph n and h	5
of influenza a viruses	5
of risk and elapsed	5
from the national institutes	5
result is representative of	5
gene set enrichment analysis	5
als p and als	5
a potential pandemic pathogen	5
for the treatment of	5
the expression of the	5
in bat roni cells	5
with acute respiratory distress	5
of ph n and	5
due to the small	5
in cells treated with	5
in convalescent subjects than	5
escape of a potential	5
with piv expressing mers	5
coronavirus associated with severe	5
either niv or hev	5
may function as a	5
were unable to detect	5
the emerging human coronavirus	5
the emergence of sars	5
isolation and characterization of	5
of the spike protein	5
are also likely to	5
infected with nl virus	5
risk and elapsed time	5
we observed a significant	5
escape of a laboratory	5
use of highly pathogenic	5
supported by nih grant	5
the absence of c	5
lab escape of a	5
mock infected or infected	5
receptor for the emerging	5
its w a mutant	5
was amplified by pcr	5
inoculated with a anhui	5
not necessarily represent the	5
loci overlapped with annotated	5
has been reported that	5
data are consistent with	5
and lower respiratory tracts	5
the mechanisms by which	5
of mouse hepatitis virus	5
genetics with a full	5
to determine if the	5
in the tigers and	5
reactive to the s	5
we have shown that	5
dmem supplemented with fbs	5
it remains to be	5
experiments were performed in	5
a significant increase in	5
viruses isolated from humans	5
the global rna virome	5
acute lung injury and	5
the importance of the	5
subunit of the spike	5
at least identical experiments	5
surveillance and pandemic preparedness	5
in hek t cells	5
of the de genes	5
for activation of rnase	5
of igg mbcs reactive	5
the binding of n	5
a lab escape of	5
plays a role in	5
pkr mrna bound to	5
the influenza virus ns	5
nih national institute of	5
were approved by the	5
to b in min	5
in the number of	5
of the m gene	5
incubation at room temperature	5
the evolution of rna	5
of the role of	5
the debate and research	5
the vast majority of	5
the s subunit of	5
representative of at least	5
the replication of the	5
in the research enterprise	5
in close proximity to	5
activation of the p	5
for the induction of	5
in the development of	5
can be used to	5
to the plasma membrane	5
in patients with severe	5
kg of body weight	5
of human immunodeficiency virus	5
including the efforts of	5
the site of infection	5
in the lethal dose	5
the upper and lower	5
the tigers and lions	5
is representative of at	5
research and reference reagent	5
was performed on a	5
in our understanding of	5
of a lab escape	5
that the absence of	5
investigated the role of	5
nsp and nsp were	5
resuspended in l of	5
time for escape of	5
dipeptidyl peptidase is a	5
the accumulation of the	5
influenza virus infection in	5
diversity of rna viruses	5
the hn dimer interface	5
and cytopathic effect of	5
elapsed time for escape	5
significant differences were observed	5
for each of the	5
the complement system is	5
see also table s	5
acquired infection from his	5
and reference reagent program	5
levels of igg against	5
signs of respiratory disease	5
the case of sars	5
the receptor binding domain	5
the urokinase pathway in	5
p and als p	5
is one of the	5
research intended to create	5
influenza virus ns protein	5
the upregulation of cd	5
in the evolution of	5
as a vaccine candidate	5
inflammatory cells in the	5
of rnase l is	5
mice immunized with combo	5
create novel potential pandemic	5
in the production of	4
in the endoplasmic reticulum	4
responsibility of the authors	4
analyzed them by immunoblotting	4
representatives from each cluster	4
cell responses to influenza	4
bat coronaviruses shows potential	4
bl j mice than	4
is a common feature	4
in dc in the	4
had no role in	4
was supported by grants	4
the to range found	4
mean od value of	4
in the activation of	4
the university of iowa	4
the adaptive immune response	4
the following binary gradient	4
cells were harvested and	4
characterized by the presence	4
lung pathology was scored	4
to determine the role	4
the infectivity and replication	4
with novel potential pandemic	4
lung samples collected from	4
also de at hpi	4
is responsible for the	4
rna loci overlapped with	4
does not seem to	4
undetected or unreported lai	4
from three independent tests	4
the h q mutation	4
binding domain of the	4
genomic characterization of a	4
nsp and nsp and	4
rna loci that overlapped	4
was replaced with fresh	4
and eptesicus fuscus skin	4
reassortant h ha h	4
on the surface of	4
was calculated using the	4
by nih grant ai	4
viral rna from the	4
of rna viruses in	4
related to those of	4
in the expression of	4
branches of rna viruses	4
glycans on the gp	4
deviation from three independent	4
h ha confers respiratory	4
identification of a novel	4
with respect to the	4
errors of the means	4
hcovs oc and e	4
ko mice compared to	4
on the value of	4
n influenza virus infection	4
the use of a	4
the absence or presence	4
is solely the responsibility	4
at least two independent	4
to estimate unknown viral	4
from different groups of	4
cytokine and chemokine protein	4
in the p x	4
directly from clinical samples	4
and its role in	4
the major branches of	4
cultured in rpmi medium	4
and influenza virus infections	4
after staining with dapi	4
better understanding of the	4
the onset of clinical	4
compared to healthy controls	4
seroevidence for h n	4
approved drugs against sars	4
activation of the complement	4
absence or presence of	4
influenza virus in ferrets	4
represent the official views	4
and e b k	4
of the predict pdf	4
innate and adaptive immunity	4
medium was added to	4
open reading frame a	4
the time of this	4
infectivity and replication of	4
was also used to	4
has been previously reported	4
of the cells were	4
the emergence of mers	4
indicate p values of	4
the probability of an	4
replication of the viral	4
influenza h ha confers	4
of h n virus	4
the icelandic veterinary institute	4
overlapped with annotated loci	4
the fact that these	4
it was shown that	4
in the lung following	4
each lab in the	4
an influx of eosinophils	4
respiratory syncytial virus infection	4
and characterization of a	4
to virus infection and	4
for h and then	4
and benefits of gain	4
were recovered from the	4
to the small number	4
as a potential therapeutic	4
numbers of putative small	4
of pkr mrna bound	4
swabs and tracheal wash	4
the fatty acid pathway	4
at the icelandic veterinary	4
the content is solely	4
n influenza infections in	4
in the perinuclear region	4
it is unclear whether	4
macaques inoculated with a	4
was calculated as the	4
was significantly higher than	4
as a surrogate for	4
virus infection in a	4
previous work has shown	4
without ca and mg	4
spr analysis of the	4
likely to work on	4
and replication of sars	4
function as a cytosolic	4
s protein of the	4
as a novel cytosolic	4
n genetic changes inventory	4
characterization of a newly	4
a reassortant h ha	4
lungs of c bl	4
at the nucleotide level	4
would be expected to	4
were fixed with formaldehyde	4
of respiratory syncytial virus	4
of circulating bat coronaviruses	4
cells as previously described	4
due to the lack	4
required for entry into	4
of group a streptococcal	4
associated with de genes	4
nt region of the	4
to a reassortant h	4
optical density at nm	4
a strategy to estimate	4
systematic review and meta	4
hhs nih national institute	4
infection with influenza a	4
cluster of circulating bat	4
an moi of and	4
of the journal or	4
confirmed the presence of	4
with influenza virus a	4
mice than in those	4
journal or of asm	4
analyses were performed using	4
higher in convalescent subjects	4
of the disease among	4
that they are modified	4
in the lgi network	4
the rate at which	4
the level of the	4
materials and methods for	4
were collected at hpi	4
entry mediated by the	4
hek t cells were	4
for molecularbased surveillance and	4
gp was expressed with	4
from inside the cells	4
biosynthesis in escherichia coli	4
of h n avian	4
analysis of the binding	4
were determined by plaque	4
by immunoblotting with antibodies	4
reduce the probability of	4
room temperature for h	4
disease in aged mice	4
levels of pkr mrna	4
titers in nasal washes	4
solely the responsibility of	4
a significant reduction in	4
immunized with pfu piv	4
expressed in hela cells	4
estimate unknown viral diversity	4
in the upper respiratory	4
necessarily represent the official	4
after min of incubation	4
s a and s	4
with bovine serum albumin	4
on pathogens with pandemic	4
cells infected with mers	4
titers in the lung	4
between nsp and nsp	4
mice immunized with inactivated	4
the t m value	4
h n and other	4
has been associated with	4
way analysis of variance	4
u ml of penicillin	4
there has been a	4
revealed the presence of	4
classes of small rnas	4
we sought to determine	4
data indicate that the	4
a novel coronavirus associated	4
a final concentration of	4
conducted in accordance with	4
when veroe cells were	4
led to the formation	4
of interferon regulatory factor	4
the c terminus of	4
escape of potential pandemic	4
respiratory syndrome coronavirus vaccine	4
the immune response to	4
supported by grants from	4
of a newly discovered	4
the inhibitory effect of	4
by a maximum of	4
we have demonstrated that	4
the ph n m	4
by quantitative reverse transcription	4
was funded by hhs	4
these data indicated that	4
a flow rate of	4
in dromedary camels in	4
seasonal h n influenza	4
the potential impact of	4
h n virus to	4
these data demonstrate the	4
newly discovered coronavirus associated	4
of at least one	4
views expressed in this	4
for min to remove	4
expressing the e protein	4
like cluster of circulating	4
infected at an moi	4
immunoblotting with antibodies to	4
n virus in ferrets	4
circulating bat coronaviruses shows	4
the common ancestor of	4
is supported in part	4
lc ms quantification of	4
of rnase l by	4
within the icelandic horse	4
the cells were cultured	4
the innate immune system	4
than in those of	4
a bacterial artificial chromosome	4
total rna was extracted	4
or influenza virus infection	4
the small number of	4
does not appear to	4
of an outbreak of	4
experimental adaptation of an	4
n influenza virus in	4
is based on the	4
cynomolgus macaques inoculated with	4
cells were lysed with	4
the spike protein of	4
such as influenza virus	4
asterisks indicate p values	4
no effect on ev	4
the views expressed in	4
for clinical signs of	4
the formation of the	4
than in unexposed subjects	4
as shown in the	4
of potential pandemic influenza	4
s and s in	4
we also examined the	4
influenza risk assessment tool	4
a newly discovered coronavirus	4
the p x ko	4
of a series of	4
the journal or of	4
as assessed by bioanalyzer	4
as a negative control	4
compared with those of	4
an influenza h ha	4
by hhs nih national	4
the dimer interface footprint	4
that are associated with	4
when march was expressed	4
were washed once with	4
added to the plate	4
as a positive control	4
where the restriction site	4
and weeks after the	4
groups were compared using	4
the construction of a	4
this is a common	4
mice were immunized with	4
of the binding of	4
of veroe tmprss cells	4
mers virus in the	4
droplet transmission to a	4
was not required for	4
ha confers respiratory droplet	4
that nsp viroporin activity	4
the world health organization	4
the viral genome and	4
care and use of	4
our data show that	4
the identification of the	4
were analyzed using the	4
an infectious cdna clone	4
the course of infection	4
the presence of grl	4
of rna viruses and	4
the supplemental material for	4
cell activation and differentiation	4
unknown viral diversity in	4
to t cell activation	4
mrna bound to ribosomes	4
the responsibility of the	4
and tracheal wash fluid	4
of the order nidovirales	4
transmission to a reassortant	4
coronaviruses shows potential for	4
has been reported to	4
than those of the	4
the official views of	4
close proximity to the	4
subjects had igg against	4
patients with severe covid	4
had igg against the	4
buffer was added to	4
a significant upregulation of	4
and replication of the	4
end of the genome	4
determine the role of	4
in such a way	4
some protection against sars	4
using the following binary	4
or mers virus in	4
the expression of gene	4
they are modified by	4
cells were obtained from	4
adaptation of an influenza	4
of human respiratory syncytial	4
trsv isolates from honeybees	4
potential pandemic influenza viruses	4
at and h postinfection	4
in an animal model	4
were detected in the	4
content is solely the	4
in the hn protein	4
caused by a novel	4
h ha h n	4
be involved in the	4
cells were washed once	4
significant upregulation of cd	4
for h n influenza	4
infected c bl mefs	4
with viral envelope glycoproteins	4
is possible that the	4
h n influenza infections	4
compounds reportedly active against	4
discovered coronavirus associated with	4
that some of the	4
in pbs containing fbs	4
the loss of gp	4
experiments with novel potential	4
acute respiratory syndrome and	4
perspective on the value	4
us porcine epidemic diarrhea	4
of respiratory disease in	4
the existence of a	4
prepared as previously described	4
evolutionary model with gamma	4
to better understand the	4
veroe cells exposed to	4
protein was detected in	4
complete genome sequence of	4
in the upper and	4
the nature of the	4
that the removal of	4
histocompatibility complex class ii	4
associated with severe acute	4
blocked the infectivity and	4
funded by hhs nih	4
primer sequences are available	4
is the probability of	4
were collected at and	4
from the plasma membrane	4
it has also been	4
viruses of the order	4
of incubation at room	4
residue of m protein	4
with p values of	4
confers respiratory droplet transmission	4
in rpmi medium supplemented	4
each of the four	4
subjects than in unexposed	4
the infectivity of sars	4
the mechanism by which	4
outbreak of severe acute	4
the role of complement	4
described in materials and	4
of the viral reads	4
of mbcs reactive to	4
ha h n virus	4
to work on influenza	4
remains to be determined	4
does not necessarily represent	4
the integral membrane fraction	4
deposited in genbank under	4
is most closely related	4
we demonstrate that sars	4
b cell response to	4
were cultured in dmem	4
influenza a virus hemagglutinin	4
related to t cell	4
strategy to estimate unknown	4
on the plasma membrane	4
the use of the	4
by the international committee	4
of mhc class i	4
of an influenza h	4
to experiments with novel	4
viral rna copy numbers	4
gene expression in the	4
views of the journal	4
one of the two	4
that overlapped with annotated	4
for the activation of	4
is important for the	4
of the immune system	4
strongly suggest that the	4
number of years that	4
for virus replication and	4
is required for the	4
a severe acute respiratory	4
and peptide from h	4
the molecular basis for	4
have been reported in	4
which has been shown	4
ethical alternatives to experiments	4
lysates were collected at	4
and analyzed them by	4
was obtained from the	4
play a critical role	4
clusters are depicted with	4
were found in the	4
with the opposing monomer	4
of the virus in	4
asymptomatic spread of sars	4
n influenza a viruses	4
response to infection with	4
differences were observed in	4
the changes in expression	4
as a receptor for	4
the de genes at	4
support the hypothesis that	4
associated with acute respiratory	4
potential for human emergence	4
that the m protein	4
that the presence of	4
epistemological perspective on the	4
increased resistance to multiple	4
by human parainfluenza virus	4
in cell culture medium	4
was added to each	4
of the test compounds	4
damage in the setting	4
with regard to the	4
influenza infections in humans	4
cells infected with sars	4
the viroporin domain is	4
g for min to	4
shows potential for human	4
distress syndrome in humans	4
the quality of the	4
against the s protein	4
onset of clinical signs	4
a systematic review and	4
pl and lps synthesis	4
were used to generate	4
the development of the	4
peptide from h n	4
avian influenza virus h	4
infection in dromedary camels	4
were normalized to the	4
the nsp nsp junction	4
the small rna locus	4
rna copy numbers were	4
the cell culture medium	4
were extracted from the	4
is likely that the	4
infected with sinv at	4
in genbank under accession	4
standard errors of the	4
collected from the same	4
each well of a	4
mice infected with the	4
p x ko mice	4
dual use research of	4
alternatives to experiments with	4
the cells were allowed	4
the impact of the	4
viral diversity in mammals	4
myotis lucifugus skin fibroblasts	4
respiratory droplet transmission to	4
mouse model of lethal	4
were performed as previously	4
the care and use	4
official views of the	4
were also de at	4
observed a significant reduction	4
had low levels of	4
cells were grown in	4
the presence of trsv	4
of the spike gene	4
function studies for molecularbased	4
patients with cryptococcal meningitis	4
following infection with sars	4
cov infectious cdna clone	4
rna was harvested from	4
sections were stained with	4
pathogenic avian influenza virus	4
were generated using the	4
and the urokinase pathway	4
with influenza a virus	4
have contributed to the	4
studies for molecularbased surveillance	4
and the development of	4
was recently shown to	4
orf and e b	4
molecularbased surveillance and pandemic	4
we also thank the	4
experiments involving pathogens with	4
sequence of the mers	4
for h at rt	4
replication in the upper	4
in unexposed subjects and	4
cells as well as	4
coronavirus associated with acute	4
are not well understood	4
cell surface heparan sulfate	4
differed by a maximum	4
at least one of	4
sequences are available upon	4
the aids research and	4
red blood cells were	4
c a and c	4
in respiratory secretions and	4
rna was purified from	4
c bl j controls	4
the evolution of the	4
a better understanding of	4
as a cytosolic pamp	4
in the levels of	4
cells were resuspended in	4
be explained by the	4
a mouse model of	4
are listed in table	4
the presence of viral	4
transiently transfected into hek	4
was performed using the	4
with the to range	4
j mice than in	4
in the dimer interface	4
on the ability of	4
in the absence or	4
respiratory distress syndrome in	4
in the right panel	4
in the ferret model	4
for the care and	4
the novelty of the	4
viruses with pandemic potential	4
of a community lai	4
cells were fixed in	4
h n genetic changes	4
increased replication of mhv	4
for the production of	4
in a model of	4
the result of a	4
with other bee viruses	4
that are involved in	3
at g ml and	3
highly susceptible to the	3
for min before being	3
the pkr protein levels	3
are not essential for	3
synthesis in escherichia coli	3
adenovirus type early region	3
of research on influenza	3
mice were infected with	3
in unexposed subjects that	3
and incubated for min	3
expressed in this article	3
with the ph n	3
the kingdom of saudi	3
cell responses against h	3
up to representatives from	3
of the complement signaling	3
targeting the boas gene	3
to confirm that the	3
gp in the golgi	3
in at least one	3
first nucleotides of the	3
copy numbers were calculated	3
cells were plated at	3
the construction of an	3
culture for viral isolation	3
branches of the tree	3
involving one or more	3
was the most likely	3
in conjunction with other	3
in case of an	3
in response to infection	3
potent activity against sars	3
were used as negative	3
by centrifugation and stored	3
treating the host response	3
the presence of m	3
for the replication of	3
igg igg c ratios	3
cells at an moi	3
of bat rnase l	3
used as negative controls	3
to further investigate the	3
infected with influenza virus	3
were determined using the	3
the members of a	3
transmission of avian influenza	3
immunized mice than in	3
patients with severe acute	3
the complete genome sequence	3
the possible role of	3
of convalescent plasma therapy	3
and research funding pause	3
levels of cytoplasmic ca	3
the most likely cause	3
the causative agent of	3
of autopsy cases from	3
of the recombinant virus	3
of bacterial and viral	3
upper as well as	3
cause of the epidemic	3
were not able to	3
these data suggested that	3
homogenized in ml of	3
responses to virus infection	3
veroe tmprss cells at	3
using an agilent bioanalyzer	3
a pneumonia outbreak associated	3
region corresponding to the	3
avian influenza h n	3
comments on the manuscript	3
the probability of a	3
supernatants were harvested and	3
of an rna virus	3
cells and veroe tmprss	3
a result of a	3
proteins of unknown function	3
disease in humans and	3
in both cell types	3
of the global rna	3
of virions from the	3
nsp and nsp of	3
subunit of the s	3
the evolutionary source of	3
a common feature of	3
that most of the	3
in the lungs and	3
the value of gain	3
host immune response to	3
dc in the ksa	3
the number of reads	3
similar to those observed	3
that the driving force	3
preparedness use of highly	3
was collected with a	3
no significant differences were	3
we did not observe	3
of the ko mice	3
days after the onset	3
is a hallmark of	3
late endosomes before uncoating	3
and said that they	3
either nsp or nsp	3
genes experiencing codon selection	3
in the background of	3
in a number of	3
these findings suggest that	3
in agreement with the	3
lung lobes and lung	3
amino acid sequence identity	3
were the first confirmed	3
and h n ha	3
replaced with fresh medium	3
sufficient to provide protection	3
fatalities and fatality burden	3
daily for clinical signs	3
the replication of trsv	3
of convalescent plasma to	3
cells were then washed	3
in the first step	3
cross sections of double	3
and stained with hematoxylin	3
and mg ml streptomycin	3
in golden syrian hamsters	3
in vitro antiviral activity	3
was also observed in	3
are not required for	3
of the influenza virus	3
outbreak of middle east	3
are consistent with the	3
mouse model of influenza	3
the university of washington	3
thermal stability of m	3
genes at hpi with	3
and analyzed by wb	3
shown in table s	3
hpi were also de	3
lg evolutionary model with	3
on at least two	3
with no close mycobacteriophage	3
recombinant inbred mouse resource	3
led to a global	3
the viral genomic rna	3
emission was collected with	3
we observed that the	3
tested how march affects	3
morbidity and mortality in	3
a high level of	3
onward transmission from a	3
in our data set	3
small rna cloning and	3
in ferrets infected with	3
a maximum of snps	3
the data representing the	3
for pkr degradation during	3
the increase in the	3
and sequenced to confirm	3
newcastle disease virus hemagglutinin	3
transfected into hek et	3
a useful tool for	3
performed on a csh	3
as in human cells	3
the human immunodeficiency virus	3
collected prior to january	3
by the university of	3
out of the er	3
were analyzed for the	3
was expressed with increasing	3
a call for a	3
animal infections in the	3
we declare no competing	3
expression of the self	3
as tlr and tlr	3
origins and evolution of	3
studies are needed to	3
junction is essential for	3
unexposed subjects that could	3
homology at the nucleotide	3
university of washington virology	3
mice at both time	3
devoid of anticoagulant activity	3
was detected with anti	3
sequencing analysis of the	3
in dmem supplemented with	3
such a way as	3
assays were performed to	3
for to h at	3
use committee of the	3
a central role in	3
humanized plasminogen mouse model	3
infectivity and cytopathic effect	3
was added at a	3
the first amino acids	3
required for virus replication	3
pandemic h n virus	3
added to the media	3
and methods for details	3
on vero e cells	3
and then incubated for	3
upregulated cd expression in	3
the m protein of	3
weeks after symptom onset	3
the accidental laboratory escape	3
and oropharyngeal swabs and	3
plays a critical role	3
the removal of glycans	3
has not yet been	3
mice infected with nl	3
the interaction of the	3
of rdrps and rts	3
the probability that a	3
between pl flux and	3
sought to determine whether	3
in virus titers in	3
provided written informed consent	3
at least one escape	3
the march antiviral activity	3
we carried out a	3
is responsible for m	3
at the bronx zoo	3
to treating patients with	3
during the evolution of	3
the probability of occurrence	3
into polarized intestinal epithelial	3
article do not necessarily	3
been deposited in the	3
such as tlr and	3
note that there are	3
group of dsrna viruses	3
respiratory syndrome coronavirus nsp	3
united states and the	3
and use committee of	3
when veroe tmprss cells	3
cloned into the same	3
receptor binding domain of	3
is required for pkr	3
have inhibitory effects on	3
porcine reproductive and respiratory	3
airborne transmission of h	3
used to generate a	3
pc a and icpedv	3
pattern recognition receptors and	3
alignment map format and	3
and there are no	3
nsp was detected with	3
were representative of the	3
were performed to detect	3
of the research enterprise	3
in the viral genome	3
funding pause on selected	3
major branches of the	3
infectious virus was rescued	3
we set out to	3
we have shown for	3
was performed using a	3
at hpi were also	3
temperature for min before	3
oropharyngeal swabs and tracheal	3
in any of the	3
veroe cells and veroe	3
inactivated fbs before use	3
coronavirus vaccine protects in	3
as a tool for	3
libraries were prepared using	3
no close mycobacteriophage relatives	3
oas protein expression in	3
to and lions to	3
tigers to and lions	3
vero cells and peritoneal	3
the elevation of ca	3
for pkr mrna by	3
significantly upregulated cd expression	3
and hpai h n	3
and virulence of an	3
was associated with the	3
of cd surface expression	3
basal oas protein expression	3
day period between may	3
contained isolates obtained from	3
the indicated time points	3
single mutations in hn	3
to the same extent	3
large number of o	3
were mock infected or	3
in vaccine virus recommendation	3
bone marrow dendritic cells	3
laboratory escape of potential	3
infected with to pfu	3
and mg to obtain	3
in the rdrp tree	3
through the use of	3
igg titers against the	3
how march affects the	3
as the expression of	3
responses against h n	3
the homologous a h	3
were submitted to the	3
were resuspended in a	3
the sequences of the	3
are required for events	3
is a receptor for	3
compared to b controls	3
farms differed by a	3
c bl primary peritoneal	3
ko mice infected with	3
samples were analyzed with	3
by the loss of	3
calculated by subtracting the	3
despite the lack of	3
study of autopsy cases	3
stability of m pro	3
molecular evolutionary genetics analysis	3
ebola virus entry requires	3
were immunized with combo	3
those of the other	3
adults and juveniles phlebotomized	3
improving pandemic influenza risk	3
potential pandemic pathogen research	3
map format and samtools	3
cells in the lung	3
does not rely on	3
of one of the	3
reverse genetics system for	3
with fetal calf serum	3
modified vaccinia virus ankara	3
differences among multiple groups	3
novel furin cleavage site	3
cells infected with sinv	3
some have argued that	3
h n and ph	3
infections in the united	3
in different cell types	3
encode a cd mimic	3
nsp and nsp suffices	3
the ph n np	3
the course of the	3
of the majority of	3
rotavirus infection in cultured	3
of novel small rnas	3
protein of human respiratory	3
the affinity of the	3
regions of the ksa	3
and resuspended in ml	3
to cell surface hs	3
on oas but not	3
with increasing amounts of	3
is involved in the	3
by grants from the	3
a score of plus	3
adoptive transfer of bmdcs	3
we note that there	3
p x receptor in	3
human cases of avian	3
the distribution of the	3
probability of at least	3
t cells were seeded	3
for middle east respiratory	3
of viral rna from	3
after h of transfection	3
of membrane phospholipid synthesis	3
is essential for the	3
titers against the s	3
of the debate and	3
ml min using the	3
each group of ferrets	3
at late time points	3
our understanding of microbial	3
of a modification on	3
and xhoi sites of	3
histopathology of the lungs	3
cannot exclude the possibility	3
of the wt mice	3
host responses to virus	3
myotis lucifugus embryonic fibroblasts	3
cells treated with the	3
has not been reported	3
that deletion of the	3
infection in cultured cells	3
and mortality in balb	3
infectious cdna clone was	3
immune response to infection	3
of fatty acid and	3
and red blood cells	3
outbreaks of the disease	3
activation of bat rnase	3
hiv activity of fimh	3
clinical and immunological assessment	3
of the viral spike	3
reduced the number of	3
were aligned to the	3
in genes experiencing codon	3
adapted rather than human	3
in neutral buffered formalin	3
views of the national	3
transduction of vero cells	3
confirmed human cases of	3
before an escape occurs	3
referred to as the	3
animal experiments were approved	3
cells stained in green	3
nsp and nsp from	3
the viral genome is	3
confirm the specificity of	3
lethal dose of sars	3
infectious cdna of the	3
the crystal structure of	3
or its w a	3
with the use of	3
at a density of	3
the branches of the	3
with antibodies to pkr	3
statistical analyses were performed	3
we did not detect	3
of the gp glycosylation	3
m pro in the	3
genes de at late	3
have been due to	3
a mixture of ketamine	3
data have been deposited	3
rnas during virus infection	3
cells were incubated at	3
the centers for disease	3
mouse resource for mapping	3
than in wt cells	3
before the emergence of	3
and sequence analysis of	3
was expressed with march	3
adult respiratory distress syndrome	3
was added to all	3
observed in the ko	3
in part by the	3
fatality burden for each	3
has been proposed that	3
presence and absence of	3
a critical role for	3
can be detected by	3
min of incubation at	3
in this article do	3
of personal protective equipment	3
of the er and	3
role of complement in	3
of the differentially expressed	3
correlated with pl flux	3
as a class pathogen	3
upregulate cd surface expression	3
was restricted to the	3
of m pro in	3
among the ph n	3
were infected with piv	3
stranded rnadependent protein kinase	3
with sinv at an	3
and rectal swabs were	3
the first nucleotides of	3
for each lab in	3
were detected in both	3
transmission from each case	3
in each well of	3
of genes of unknown	3
upregulation of cd surface	3
both niv and hev	3
lyse at room temperature	3
used to block the	3
virus in ferrets and	3
we confirmed that the	3
a single dose of	3
mortality in balb c	3
in a previous report	3
in cultured cells and	3
geo accession number gse	3
as shown in a	3
posttranslational control of plsb	3
release of viral rna	3
systematic assembly of a	3
of cd is a	3
as described in materials	3
viral isolates were sequenced	3
of gp and gp	3
were compared using the	3
the pause on gain	3
that can be used	3
this result suggests that	3
rna was isolated from	3
of washington virology laboratory	3
induced by rotavirus infection	3
b and peptide from	3
between h n and	3
function deliberative process and	3
loss of gp n	3
and the role of	3
as well as lower	3
detected by confocal microscopy	3
rectal and nasal swabs	3
the membrane protein of	3
in the formation of	3
rates and empirical amino	3
despite the fact that	3
bl primary peritoneal macrophages	3
distributed site rates and	3
absence of complement signaling	3
has previously been shown	3
in human a cells	3
the results obtained with	3
function experiments with pathogens	3
was analyzed using a	3
interactions with the backbone	3
to be affected by	3
insensitive to periodate oxidation	3
rnase l is dependent	3
syndrome coronavirus infection in	3
veroe and veroe tmprss	3
israeli acute paralysis virus	3
assay was conducted to	3
the blood transfusion centre	3
is present in the	3
for many rna viruses	3
for more than generations	3
through the aids research	3
that the host immune	3
analysis was performed to	3
vaccine virus recommendation and	3
experiments that alter pathogens	3
that is required for	3
to be active against	3
the increased doses of	3
nsp nsp junction is	3
ma infection activates the	3
autopsy cases from singapore	3
between p and p	3
in a vacuum centrifuge	3
on a csh c	3
a high mortality rate	3
from each of the	3
virus and mycobacterium tuberculosis	3
experiments were approved by	3
the sequence alignment map	3
min using the following	3
pause on selected gain	3
are depicted with a	3
vaccines and antivirals are	3
with the activation of	3
observed in the tomograms	3
oc s igg titers	3
polarized intestinal epithelial cells	3
of the host immune	3
in the majority of	3
old c bl mice	3
candidate loci for putative	3
containing the accidental laboratory	3
an avian h n	3
ebov entry into macrophages	3
costs of the pause	3
detected by northern blot	3
letter to the editor	3
tmprss cells at hpi	3
cov or influenza virus	3
as mild to marked	3
an moi of pfu	3
under accession numbers mt	3
noncoding rna expression in	3
any of the samples	3
rectal swabs were collected	3
design and synthesis of	3
an equal volume of	3
understanding of microbial pathogenesis	3
samples from mice infected	3
s and n proteins	3
respiratory and fecal samples	3
in c bl mice	3
significantly higher in combo	3
on ice for min	3
for at least days	3
in a murine model	3
from mice infected with	3
pneumonia outbreak associated with	3
gene expression profiles in	3
suggesting that it may	3
is known that the	3
plays an important role	3
of ht sequencing coverage	3
not provide sterilizing immunity	3
inbred mouse resource for	3
pkr mrna levels and	3
higher than that seen	3
we hypothesized that the	3
of the structural proteins	3
transmission of mers coronavirus	3
virus entry requires the	3
with virus replication in	3
not required for entry	3
national academy of sciences	3
for the selected adhesins	3
lungs of the p	3
potential pandemic pathogen experiments	3
to be differentially expressed	3
sinv at an moi	3
in terms of the	3
to the best of	3
of the n gene	3
g of total rna	3
type i interferon production	3
did not provide sterilizing	3
experiments with pathogens of	3
the antigen and pbs	3
culture medium was replaced	3
and empirical amino acid	3
to confirm the specificity	3
assayed for pkr mrna	3
were used for the	3
data set s b	3
viruses due to the	3
from the blood transfusion	3
sensitive to endo h	3
the epidemic of respiratory	3
apical surface of polarized	3
used to determine the	3
was cotransfected with g	3
myotis velifer embryonic fibroblasts	3
of the antigen presentation	3
each cluster were selected	3
deliberative process and research	3
indicating that m protein	3
cells were plated in	3
mice than in c	3
cells were infected at	3
against invasive gas infection	3
the presence and absence	3
of these small rnas	3
case fatality rate of	3
protects in an aged	3
m protein rather than	3
the ko mice compared	3
were cultured in the	3
time to an escape	3
we also observed increased	3
lethal and sublethal doses	3
movies s and s	3
were consistently upregulated or	3
class i fusion proteins	3
this study represents the	3
the lungs of virus	3
sequences obtained from sars	3
the middle east and	3
of the e gene	3
based on our previous	3
and incorporation into virions	3
in an rna virus	3
lacking the p x	3
the evolutionary provenance of	3
viral rna into the	3
a novel furin cleavage	3
ca and mg to	3
first immunoprecipitated with anti	3
in the urokinase pathway	3
of the orf ab	3
cotransfected with g of	3
detection and analysis of	3
including microrna host genes	3
the nsp nsp cleavage	3
well plate h before	3
at least in part	3
at days and postinfection	3
was conducted to assay	3
small rnas such as	3
provide protection against e	3
outbreak associated with a	3
had high levels of	3
protein rather than m	3
of small rna loci	3
were differentially expressed during	3
for the sequences of	3
model of lethal disease	3
the plates were incubated	3
of cytokines and chemokines	3
results are consistent with	3
available in table s	3
in ferret passage experiments	3
and cloned into the	3
mhv p and mhv	3
alter pathogens with pandemic	3
upregulation of cd is	3
found to be differentially	3
droplet transmission in ferrets	3
conjunction with other bee	3
most probably due to	3
least one of the	3
three times for min	3
detect the accumulation of	3
qs at g ml	3
were lower than those	3
community lai leads to	3
dna was purified from	3
were downloaded from the	3
the h n strain	3
infected with a lethal	3
cells were stimulated with	3
we found that a	3
shows that addition of	3
gene encodes a protein	3
have been used to	3
pandemic preparedness use of	3
and viral titers were	3
the research enterprise is	3
the area of the	3
were infected with the	3
added to l of	3
a diverse array of	3
between may and october	3
deletion of its trs	3
exudative phase of dad	3
of the virus and	3
lab in the enterprise	3
first time that the	3
to the small rna	3
group were euthanized at	3
limited airborne transmission of	3
using differential scanning fluorimetry	3
expressed with increasing amounts	3
contact with the opposing	3
provide insight into the	3
detected in nasal swabs	3
was determined using the	3
was used to block	3
e in the supplemental	3
an undetected or unreported	3
be active against sars	3
was detected in the	3
appear to have been	3
and cd t cells	3
of trsv in the	3
were allowed to lyse	3
nsp junction is essential	3
class of small rnas	3
a new coronavirus of	3
plate h before transfection	3
in dmem containing heat	3
compared to the wild	3
to the dimer interface	3
was performed by adding	3
that the s protein	3
length gp and gp	3
with the antigen and	3
for the purpose of	3
the amino acid level	3
important role in the	3
similar to those in	3
were immunized with pfu	3
the yakult central institute	3
the structure of the	3
the apical surface of	3
during the nrc symposium	3
ph n np pool	3
and influenza virus infection	3
regulation of the antigen	3
rna into the cell	3
collected from h n	3
are rarely used in	3
data were normalized to	3
of a number of	3
the intermediate plasmid pbac	3
washed once with pbs	3
be detected in the	3
and dominant negative mutants	3
and l of the	3
immunized with inactivated mers	3
aivs among the ph	3
ngs within the gp	3
bat rnase l depends	3
elapsed time to an	3
and postinfection in the	3
was due to a	3
of the probability of	3
closely related to the	3
that the majority of	3
absence of test compounds	3
by pattern recognition receptors	3
the formation of double	3
the seasonal prevalence of	3
cytokines and chemokines in	3
activation and differentiation were	3
a virus between ferrets	3
p values of less	3
could be detected in	3
recently reported that the	3
expressed as a percentage	3
transcription and replication of	3
pathogenesis of infectious diseases	3
to nt in length	3
data available in the	3
the removal of n	3
signal regulatory protein alpha	3
pkr mrna by qpcr	3
increased delivery of sitraf	3
role in vaccine virus	3
than that seen with	3
on the apical surface	3
were cloned by three	3
with pfu mers ma	3
chance of an escape	3
cis and in trans	3
influenza a virus between	3
may be used to	3
of infected mice were	3
new coronavirus of probable	3
were added to the	3
is the set of	3
in vero a cells	3
to be important for	3
codons that are rarely	3
compared to the wt	3
approved by the university	3
site rates and empirical	3
host response to viral	3
times higher than the	3
infection of a human	3
the development of an	3
the biogenesis of coronavirus	3
coronavirus causes disease and	3
the time of infection	3
infection is inhibited by	3
at the amino acid	3
tested positive for sars	3
the presence of sars	3
in the intestines of	3
high expression levels of	3
primary cd t cell	3
causes disease and mortality	3
were to times higher	3
pbs without ca and	3
of various concentrations of	3
c terminus of the	3
ev infection is inhibited	3
the case for the	3
at the indicated time	3
school of medicine at	3
disease in horses and	3
might be able to	3
as defined by the	3
onward transmission from each	3
in patients with hiv	3
were purchased from sigma	3
most likely cause of	3
genes related to t	3
in tigers and lions	3
mirna expression changes in	3
the genomic region corresponding	3
gp shedding and secretion	3
g for min at	3
the humanized plasminogen mouse	3
more mouse strains during	3
the upregulation of the	3
reduction in virus titers	3
probability of occurrence of	3
were collected from each	3
a part of the	3
a factor of to	3
at the cell surface	3
min to remove debris	3
well as lower respiratory	3
d b and peptide	3
s proteins of the	3
isolates from horses at	3
with a new coronavirus	3
pierce ripa lysis buffer	3
in the ph n	3
was calculated by subtracting	3
said that they were	3
on the same strand	3
control proteins h and	3
funders had no role	3
the average log fc	3
type response against invasive	3
of probable bat origin	3
against h n and	3
also table s in	3
and cellular immune responses	3
from each case of	3
that are rarely used	3
homologous a h n	3
cells is dependent on	3
at later times postinfection	3
both hek and vero	3
staff of the university	3
wt and d g	3
sequences were used to	3
it is unlikely that	3
influenza a virus and	3
average log fc was	3
small rna libraries were	3
the largest rna virus	3
levels of ht sequencing	3
from a total of	3
was identified in the	3
and juveniles phlebotomized in	3
infection of c bl	3
our previous findings in	3
it is clear that	3
within each of the	3
isolates were recovered from	3
was present in the	3
virion structure and assembly	3
the function of the	3
the upper respiratory tract	3
approach to treating patients	3
was not affected by	3
cdc qpcr probe assay	3
may be sufficient to	3
coxsackievirus and adenovirus receptor	3
converted to a particles	3
to activate rnase l	3
data suggest that the	3
region of the orf	3
were not statistically significant	3
either boas or boas	3
as a consequence of	3
exposed to ev or	3
a p value of	3
higher than that of	3
centrifugation for min at	3
most of the cells	3
by the fact that	3
to a broad range	3
t cells were transfected	3
required for the formation	3
and public health experts	3
between and weeks after	3
ev entry depends on	3
both animal and human	3
by h in culture	3
of the viral genomic	3
respiratory syndrome coronavirus replication	3
a novel cytosolic pamp	3
see text s in	3
the number of connections	3
was significantly lower than	3
read sequences that mapped	3
in the spike gene	3
burden for each lab	3
were added to a	3
to the viral genome	3
is dependent on the	3
stained with crystal violet	3
the study of viral	3
this article do not	3
allowed to lyse at	3
viruses were cloned by	3
and immunological assessment of	3
not appear to be	3
the accumulation of svsrna	3
nucleotides of the viral	3
the virus in the	3
global alert and response	3
lacking the structural e	3
exclude the possibility that	3
time for a chance	3
l depends on oas	3
sister group to the	3
of boas and boas	3
were predicted to be	3
to examine cd expression	3
loci for putative small	3
each case of lai	3
assembly of a full	3
supernatants were harvested at	3
cells were lysed by	3
followed by min of	3
of polarized epithelial cells	3
infected lysates at hpi	3
downregulated at hpi by	3
and experiments were performed	3
supplemented with fetal calf	3
de at late times	3
that are in place	3
for min on ice	3
rnase l is a	3
c in the supplemental	3
we also found that	3
at several time points	3
the mers s protein	3
cells were transfected using	3
response against invasive gas	3
centrifugation and stored at	3
most recent common ancestor	3
have been suggested to	3
was sufficient to provide	3
were grown to confluence	3
to the mouse reference	3
no significant change in	3
mm dipotassium phosphate buffer	3
a novel human coronavirus	3
for the detection of	3
we further examined the	3
for the transmission of	3
moves to early and	3
tissues of the urogenital	3
were transiently transfected with	3
acute respiratory syndrome cov	3
and peripheral blood mononuclear	3
from an animal reservoir	3
delivery of the m	3
analysis of the same	3
does not affect the	3
virus recommendation and pandemic	3
for min each time	3
in the form of	3
of dsrna viruses that	3
results demonstrate that the	3
period between may and	3
presence of various concentrations	3
tissue culture infective doses	3
of the international committee	3
glycan cap and mld	3
preparation of the manuscript	3
the nsp viroporin domain	3
in the treatment of	3
a recombinant inbred mouse	3
were incubated overnight at	3
as the number of	3
the conserved s subunit	3
lai leads to a	3
findings in human cells	3
after removal of the	3
and innate immune responses	3
a csh c column	3
the influenza risk assessment	3
screennc identified out of	3
of mice infected with	3
have a role in	3
supplemented with g ml	3
to representatives from each	3
with co for h	3
in the regulation of	3
the lethal and sublethal	3
analysis was performed using	3
endemic within the icelandic	3
of serum and virus	3
in a previous study	3
the viruses and their	3
m protein of sars	3
the herpes simplex virus	3
a review of the	3
pkr protein levels were	3
mouse adenovirus type early	3
transfer of bmdcs from	3
to the sars coronavirus	3
have been associated with	3
assembly of the full	3
passaged in dmem containing	3
yakult central institute for	3
susceptible to the infectivity	3
rnas of viral origin	3
induce the formation of	3
ninth report of the	3
were euthanized on day	3
in cells pretreated with	3
of neutrophils in the	3
by centrifugation for min	3
largest rna virus genome	3
as well as some	3
prevalence of trsv in	3
ev infection of caco	3
and purified using a	3
h n or h	3
humoral responses against sars	3
reviewed in references and	3
in h n and	3
replication in cell cultures	3
titers were determined in	3
virus and hendra virus	3
cd is a host	3
immunization with combo alum	3
vagueness and costs of	3
three times with pbs	3
the staff of the	3
neutrophils in the lungs	3
sufficient to induce dmv	3
number of uniquely mapped	3
mice were used for	3
from a case of	3
among the members of	3
the loss of the	3
for porcine epidemic diarrhea	3
n or h n	3
counted the number of	3
being harvested and centrifuged	3
it is not clear	3
analysis of deformed wing	3
were seeded in each	3
in the same way	3
the differences between the	3
it is also possible	3
viral rna synthesis was	3
based vaccine expressing the	3
values of less than	3
inhibited ev infection of	3
of the evolution of	3
are thought to be	3
the env and ha	3
the fact that the	3
for putative small rnas	3
results indicate that ev	3
roni cells is dependent	3
mixed with the antigen	3
the circular profiles observed	3
red infectious center assays	3
mice lacking the p	3
assessment of asymptomatic sars	3
superscript ii reverse transcriptase	3
as a loading control	3
to the activation of	3
of mutations in nsp	3
predicted with secondary structures	3
broad antiviral activity by	3
with lymphocytic choriomeningitis virus	3
in cell culture and	3
were transiently transfected into	3
also data not shown	3
l is dependent on	3
from the national institute	3
and research pause have	3
of a severe acute	3
the e gene was	3
have been described previously	3
empirical amino acid frequencies	3
this project was funded	3
multiplicity of infection of	3
personal protective equipment and	3
cases of avian influenza	3
as in wt cells	3
was supported in part	3
the sensing of pathogen	3
min before being harvested	3
observed differential expression of	3
the production of svsrnas	3
that it is a	3
were performed as described	3
that were representative of	3
of the purinergic receptor	3
data collection and analysis	3
a wide variety of	3
pandemic influenza risk assessment	3
igg c titers were	3
and were passaged in	3
approved by the unc	3
on the expression of	3
of both host and	3
of its trs and	3