This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.
quadgram | frequency |
---|---|
severe acute respiratory syndrome | 212 |
porcine epidemic diarrhea virus | 144 |
acute respiratory syndrome coronavirus | 113 |
in the presence of | 91 |
in vero e cells | 88 |
cells were infected with | 80 |
of severe acute respiratory | 66 |
of porcine epidemic diarrhea | 58 |
infected vero e cells | 47 |
as shown in fig | 43 |
for the treatment of | 42 |
vero cells were infected | 40 |
in vitro and in | 38 |
in the present study | 38 |
at an moi of | 38 |
in the absence of | 38 |
the antiviral activity of | 37 |
cells were treated with | 37 |
vitro and in vivo | 36 |
african green monkey kidney | 35 |
on vero e cells | 35 |
coronavirus infectious bronchitis virus | 35 |
of the s protein | 34 |
the severe acute respiratory | 32 |
washed three times with | 32 |
herpes simplex virus type | 31 |
of vero e cells | 30 |
the cells were fixed | 30 |
the intracellular extracts of | 29 |
cells were fixed with | 29 |
cells were incubated with | 29 |
gds and gds strains | 28 |
with severe acute respiratory | 28 |
with fetal bovine serum | 27 |
a and icpc a | 27 |
icpc a and icpc | 27 |
cells were washed twice | 27 |
rna was extracted from | 27 |
middle east respiratory syndrome | 27 |
vero cells infected with | 26 |
in the united states | 25 |
on the other hand | 25 |
were washed twice with | 25 |
the cells were washed | 25 |
was used as a | 25 |
as well as the | 24 |
the vero cell line | 24 |
has been shown to | 24 |
the gds and gds | 23 |
for the production of | 23 |
at c for h | 23 |
in cells infected with | 23 |
after h of incubation | 23 |
porcine reproductive and respiratory | 22 |
as a negative control | 22 |
in the case of | 22 |
determined by plaque assay | 22 |
supplemented with fetal bovine | 22 |
epidemic diarrhea virus in | 22 |
reproductive and respiratory syndrome | 22 |
the vero cells and | 21 |
was added to the | 21 |
effect on vero e | 21 |
used in this study | 21 |
the recombinant ibvs and | 21 |
of the viral genome | 20 |
and respiratory syndrome virus | 20 |
s s cleavage site | 20 |
washed twice with pbs | 20 |
at a moi of | 20 |
total rna was extracted | 19 |
an important role in | 19 |
incubated for h at | 19 |
pcr and western blotting | 19 |
vero e cells were | 19 |
cells were inoculated with | 19 |
east respiratory syndrome coronavirus | 19 |
presence or absence of | 19 |
at the plasma membrane | 19 |
and vero cells were | 19 |
at a multiplicity of | 19 |
a multiplicity of infection | 19 |
intracellular extracts of ln | 18 |
in vero cells and | 18 |
added to each well | 18 |
were washed three times | 18 |
cells were washed with | 18 |
cells were seeded in | 18 |
cells were fixed and | 18 |
the presence or absence | 18 |
h at room temperature | 18 |
of blfp crude extract | 18 |
recombinant ibvs and beaudette | 18 |
three times with pbs | 18 |
vero e cells exposed | 17 |
for the detection of | 17 |
the coronavirus infectious bronchitis | 17 |
avian infectious bronchitis virus | 17 |
vero cells were seeded | 17 |
isolation and characterization of | 17 |
in the vero cells | 17 |
cell fusion activity of | 17 |
e cells exposed to | 17 |
of a novel coronavirus | 17 |
at different time points | 17 |
in the presence or | 17 |
were washed with pbs | 17 |
the s s cleavage | 16 |
propagated in vero cells | 16 |
the cells were incubated | 16 |
vero cells and ipec | 16 |
pcd and vero cells | 16 |
for h at room | 16 |
infectivity of icpc a | 16 |
for the development of | 16 |
the pedv s protein | 16 |
the vero e cells | 16 |
cells were transfected with | 16 |
cells infected with the | 16 |
pedv in vero cells | 16 |
african swine fever virus | 16 |
was added to each | 15 |
plates were infected with | 15 |
the s protein of | 15 |
these results indicated that | 15 |
min at room temperature | 15 |
than that of the | 15 |
egfr pi k akt | 15 |
at a concentration of | 15 |
the inoculum was removed | 15 |
the porcine epidemic diarrhea | 15 |
these results suggest that | 15 |
vero e and ma | 15 |
in the s protein | 15 |
cells in the presence | 15 |
was used as the | 15 |
vero and a cells | 15 |
it is important to | 15 |
the spike protein of | 15 |
the sam binding site | 15 |
the expression levels of | 15 |
in pbs for min | 15 |
vero e cells in | 14 |
at room temperature for | 14 |
and the cells were | 14 |
infected with wild type | 14 |
vero e cells and | 14 |
used as a negative | 14 |
a member of the | 14 |
the antiviral effect of | 14 |
was performed as described | 14 |
with different concentrations of | 14 |
was removed and the | 14 |
were fixed and stained | 14 |
incubated at c for | 14 |
in the treatment of | 14 |
acute respiratory syndrome in | 14 |
vero cells treated with | 14 |
the results showed that | 14 |
treated with or without | 14 |
in cells transfected with | 14 |
there was no significant | 13 |
in the sam binding | 13 |
one of the most | 13 |
the infectivity of icpc | 13 |
in the vero cell | 13 |
cytopathic effect on vero | 13 |
of herpes simplex virus | 13 |
and characterization of a | 13 |
the cells were collected | 13 |
for min at room | 13 |
patients with severe acute | 13 |
is one of the | 13 |
the invasion rates of | 13 |
at hpi and hpi | 13 |
at the same time | 13 |
of the mutant virus | 13 |
vero cells were treated | 13 |
were incubated for h | 13 |
c in a co | 13 |
viral rna was extracted | 13 |
were infected with pedv | 13 |
inoculum was removed and | 13 |
infection of vero cells | 13 |
green monkey kidney cells | 12 |
infection in vero cells | 12 |
with pedv strains at | 12 |
cells were grown in | 12 |
with the recombinant ibvs | 12 |
porcine epidemic diarrhoea virus | 12 |
cells were incubated for | 12 |
is a member of | 12 |
in vero cells infected | 12 |
in vero e and | 12 |
the infectivity of the | 12 |
of the gds and | 12 |
vero cells were inoculated | 12 |
were infected with hsv | 12 |
at rt for min | 12 |
had no effect on | 12 |
regulation of cell death | 12 |
in the number of | 12 |
associated with severe acute | 12 |
been shown to be | 12 |
characterization of a novel | 12 |
monolayers of vero cells | 12 |
well plates were infected | 12 |
with wild type and | 12 |
the in vitro prophylactic | 12 |
the expression of the | 12 |
the authors declare that | 12 |
with the intracellular extracts | 12 |
these results suggested that | 12 |
were determined by plaque | 12 |
cells transfected with the | 11 |
avian coronavirus infectious bronchitis | 11 |
the presence of increasing | 11 |
this work was supported | 11 |
the vero e and | 11 |
of the coronavirus infectious | 11 |
of the rescued virus | 11 |
d d d d | 11 |
a fixed concentration of | 11 |
cells were cultured in | 11 |
for min at rt | 11 |
a novel coronavirus associated | 11 |
infected vero cells were | 11 |
compared to those of | 11 |
on the surface of | 11 |
of infectious bronchitis virus | 11 |
as compared to the | 11 |
fixed concentration of sars | 11 |
cells were maintained in | 11 |
the treatment of sars | 11 |
the development of a | 11 |
the production of viral | 11 |
coronavirus associated with severe | 11 |
cells were pretreated with | 11 |
to those of the | 11 |
to a fixed concentration | 11 |
fusion activity of s | 11 |
was observed in the | 11 |
treated with different concentrations | 11 |
of vero cells with | 11 |
compared to the parental | 11 |
was determined using the | 11 |
porcine epidemic diarrhea in | 11 |
monkey kidney cell line | 11 |
cells were collected at | 11 |
against pedv in vero | 11 |
the invasion efficiency of | 11 |
novel coronavirus associated with | 11 |
infected with pedv strains | 11 |
were infected with the | 11 |
length cdna clone of | 11 |
were detected in the | 11 |
cells infected with wild | 11 |
pi k akt pathway | 11 |
and incubated for h | 11 |
the vero and ipec | 11 |
fusion activity of the | 11 |
propagation in vero cells | 11 |
in vero cells in | 11 |
at a density of | 11 |
our results suggest that | 11 |
as a result of | 11 |
were collected at hpi | 11 |
cells exposed to a | 11 |
exposed to a fixed | 11 |
the vero cells were | 11 |
lower than that of | 11 |
were fixed with paraformaldehyde | 11 |
the presence of ribavirin | 10 |
presence of increasing concentrations | 10 |
carrying mutations in the | 10 |
the vero cell lineage | 10 |
a concentration ranging from | 10 |
centers for disease control | 10 |
the end of the | 10 |
at a concentration ranging | 10 |
the plasma membrane and | 10 |
rates of the gds | 10 |
pseudovirions with uncleaved s | 10 |
it is possible that | 10 |
washed with pbs and | 10 |
results showed that the | 10 |
m f and c | 10 |
used at a concentration | 10 |
american type culture collection | 10 |
of the recombinant ibvs | 10 |
against herpes simplex virus | 10 |
mutations in the sam | 10 |
and then incubated with | 10 |
an equal volume of | 10 |
of peptide h on | 10 |
the vero e cell | 10 |
in vitro prophylactic effects | 10 |
the study of the | 10 |
of increasing concentrations of | 10 |
the early stages of | 10 |
were observed in the | 10 |
replication in vero e | 10 |
was carried out by | 10 |
was extracted from the | 10 |
of the murine coronavirus | 10 |
g a and rmv | 10 |
of the spike protein | 10 |
g for min at | 10 |
the presence of trypsin | 10 |
fixed and stained with | 10 |
in patients with severe | 10 |
with ck ch ldl | 10 |
have been shown to | 10 |
of prrsv and pedv | 10 |
supernatants were collected at | 10 |
and in the ipec | 10 |
propagation of the virus | 10 |
and in the vero | 10 |
at h post infection | 10 |
were inoculated with kpedv | 10 |
mechanism of action of | 10 |
cells were washed three | 10 |
work was supported by | 10 |
vero cells were cultured | 10 |
at the end of | 10 |
these data suggest that | 10 |
containing the empty vector | 10 |
identification of a novel | 10 |
were inoculated with the | 10 |
in the footpad with | 10 |
bile and bile acids | 10 |
of the porcine epidemic | 10 |
culture supernatants were collected | 10 |
proteolytic cleavage of the | 10 |
room temperature for h | 10 |
vero cells were grown | 10 |
of the in vitro | 10 |
in the culture medium | 10 |
at h after the | 10 |
the inhibitory effect of | 10 |
infectious cdna clone of | 10 |
studies have shown that | 10 |
be due to the | 9 |
in vitro antiviral activity | 9 |
and then infected with | 9 |
apoptosis in vero e | 9 |
p mapk signaling pathway | 9 |
the cells were then | 9 |
the overexpression of the | 9 |
at the time of | 9 |
was amplified by rt | 9 |
cells containing the empty | 9 |
after washing three times | 9 |
of vero cells were | 9 |
of sabin poliovirus type | 9 |
production of viral vaccines | 9 |
of the viral rna | 9 |
of the virus of | 9 |
was observed in cells | 9 |
epidemic diarrhea in cell | 9 |
strains at h after | 9 |
when compared to the | 9 |
from patients recovered from | 9 |
to that of the | 9 |
rna was isolated from | 9 |
for disease control and | 9 |
it was found that | 9 |
the p mapk signaling | 9 |
by plaque assay and | 9 |
of the helicase protein | 9 |
and gds strains were | 9 |
the regulation of cell | 9 |
fusion of the viral | 9 |
of pedv icpc a | 9 |
ns a and ns | 9 |
antiviral activity of ribavirin | 9 |
of mouse hepatitis virus | 9 |
invasion efficiency of pedv | 9 |
error bars represent standard | 9 |
e protein mab g | 9 |
without blfp crude extract | 9 |
the results of the | 9 |
al kassaa et al | 9 |
cells were overlaid with | 9 |
sialic acid binding activity | 9 |
pedv icpc a and | 9 |
epidemic diarrhea virus strains | 9 |
of the sars coronavirus | 9 |
the virus of porcine | 9 |
may be due to | 9 |
of icpc a and | 9 |
crude extract against pedv | 9 |
by the addition of | 9 |
was found to be | 9 |
of cell death during | 9 |
suggesting that myricetin may | 9 |
activation of p mapk | 9 |
the growth properties of | 9 |
as described above and | 9 |
diarrhea in cell culture | 9 |
blfp crude extract against | 9 |
the ns a protein | 9 |
the focus forming assay | 9 |
and vero e cells | 9 |
well tissue culture plates | 9 |
vero e cell line | 9 |
virus of porcine epidemic | 9 |
we determined that the | 9 |
the results indicated that | 9 |
added to the cells | 9 |
the cells were infected | 9 |
infection in vero e | 9 |
incubated at room temperature | 9 |
flavivirus e protein mab | 9 |
the infected cells were | 9 |
mers pseudovirions with uncleaved | 9 |
vero e cells are | 9 |
at the indicated times | 9 |
associated with diarrhea in | 8 |
disease control and prevention | 8 |
green monkey kidney cell | 8 |
by the severe acute | 8 |
infected with an moi | 8 |
vero cells and and | 8 |
and r l mutant | 8 |
of african green monkey | 8 |
cells and and in | 8 |
epidemic diarrhea virus infection | 8 |
was significantly lower than | 8 |
fixed with paraformaldehyde in | 8 |
the total number of | 8 |
it was shown that | 8 |
in cell cultures and | 8 |
blood samples were collected | 8 |
type i and type | 8 |
washing three times with | 8 |
feline infectious peritonitis virus | 8 |
were observed in cells | 8 |
of the viral envelope | 8 |
the two cell lines | 8 |
results showed that pedv | 8 |
an african green monkey | 8 |
absence of growth factors | 8 |
syndrome coronavirus spike protein | 8 |
myricetin may interact with | 8 |
s protein of pedv | 8 |
in these cell lines | 8 |
and hpi for qrt | 8 |
a novel coronavirus from | 8 |
cells were stained with | 8 |
for h at c | 8 |
cells were incubated at | 8 |
the african green monkey | 8 |
results indicated that the | 8 |
and all of them | 8 |
aminopeptidase n is a | 8 |
e and huh cells | 8 |
the absence of growth | 8 |
passages in cell culture | 8 |
was no significant difference | 8 |
a and ns b | 8 |
line of african green | 8 |
similar to that of | 8 |
induction of apoptosis by | 8 |
is thought to be | 8 |
pedv strains at h | 8 |
medium was removed and | 8 |
collected at hpi and | 8 |
the induction of apoptosis | 8 |
of three independent experiments | 8 |
for the inhibition of | 8 |
were considered statistically significant | 8 |
abortus and chlamydia pecorum | 8 |
syndrome in hong kong | 8 |
institutional animal care and | 8 |
experimental infection of pigs | 8 |
were infected with an | 8 |
vero cells grown in | 8 |
protein of severe acute | 8 |
was determined relative to | 8 |
in each group were | 8 |
in vero and ipec | 8 |
acute respiratory distress syndrome | 8 |
care and use committee | 8 |
respiratory syndrome in hong | 8 |
were infected with a | 8 |
on the infection of | 8 |
respiratory syndrome coronavirus spike | 8 |
the viral titers in | 8 |
the world health organization | 8 |
r l mutant viruses | 8 |
authors declare that they | 8 |
the plates were incubated | 8 |
and and in the | 8 |
intracellular extracts of ypk | 8 |
animal care and use | 8 |
culture supernatants were harvested | 8 |
a functional receptor for | 8 |
viral rna synthesis and | 8 |
the medium was removed | 8 |
at various time points | 8 |
glycoprotein of murine coronavirus | 8 |
and infected with pedv | 8 |
was determined by plaque | 8 |
the international committee on | 8 |
variants of porcine epidemic | 8 |
fold serial dilutions of | 8 |
were determined using the | 8 |
e cells in the | 8 |
with an moi of | 8 |
chlamydia abortus and chlamydia | 8 |
twice with pbs and | 8 |
phosphorylation of p mapk | 8 |
acquisition of the cell | 8 |
i and type iii | 8 |
in vero cells at | 8 |
infected vero cells treated | 8 |
the detection limit of | 8 |
no significant difference between | 8 |
vero e and huh | 8 |
cells and incubated at | 8 |
functional receptor for the | 8 |
invasion rates of the | 8 |
with vero e cells | 8 |
is not essential for | 8 |
as described in the | 8 |
with or without myricetin | 8 |
the s gene of | 8 |
genes in vero cells | 8 |
for h and then | 8 |
in vero cells were | 8 |
h of incubation at | 8 |
hpi and hpi for | 8 |
in cell culture and | 8 |
isolated from kefir grains | 8 |
is a functional receptor | 8 |
virus entry and syncytia | 8 |
entry and syncytia formation | 8 |
an infectious cdna clone | 8 |
spike protein of sars | 8 |
that they have no | 8 |
are shown in fig | 8 |
for the first time | 8 |
for h at rt | 8 |
genome sequence of the | 8 |
pbs for min at | 8 |
of porcine epidemic diarrhoea | 8 |
infected cells were treated | 8 |
the virus life cycle | 8 |
significantly lower than that | 8 |
incubated with pedv at | 8 |
declare that they have | 8 |
there is an urgent | 8 |
at the s s | 8 |
was propagated in vero | 7 |
the growth kinetics of | 7 |
these results demonstrate that | 7 |
and western blotting at | 7 |
ic value of this | 7 |
no effect on the | 7 |
treatment of zikv infection | 7 |
infected cells were detected | 7 |
the virus inoculum was | 7 |
at least three independent | 7 |
genomic rna and sg | 7 |
pretreated with the intracellular | 7 |
in lung epithelial cells | 7 |
in cells expressing s | 7 |
of no and peroxynitrite | 7 |
cell infection by pedv | 7 |
to each well and | 7 |
that of the parental | 7 |
j cells were transfected | 7 |
infected cells were fixed | 7 |
of the virus in | 7 |
cell lines were infected | 7 |
on taxonomy of viruses | 7 |
were significantly higher than | 7 |
infected with chikv ls | 7 |
the viral spike protein | 7 |
vero cells with the | 7 |
in a variety of | 7 |
vero e cells infected | 7 |
an urgent need to | 7 |
a single amino acid | 7 |
of vero cells on | 7 |
proteolytic processing of the | 7 |
of the virus to | 7 |
they have no competing | 7 |
on the expression of | 7 |
cell cycle progression at | 7 |
is consistent with the | 7 |
as shown in figure | 7 |
were incubated with pedv | 7 |
have no competing interests | 7 |
tcid ml at hpi | 7 |
was used for the | 7 |
with virus gd protein | 7 |
test the invasion efficiency | 7 |
the cell monolayers were | 7 |
within the helicase protein | 7 |
novel coronavirus in patients | 7 |
with gds or gds | 7 |
is an urgent need | 7 |
the replication of the | 7 |
of the icpc a | 7 |
observed in cells expressing | 7 |
is known to be | 7 |
and western blotting assay | 7 |
rna was extracted using | 7 |
in a co atmosphere | 7 |
days two and four | 7 |
international committee on taxonomy | 7 |
well plates until confluence | 7 |
functional characterization of the | 7 |
to the wt sequence | 7 |
as well as to | 7 |
was performed using the | 7 |
stage of zikv infection | 7 |
in the s region | 7 |
mutations in the s | 7 |
epidemic diarrhea virus strain | 7 |
of vesicular stomatitis virus | 7 |
in both vero and | 7 |
a high level of | 7 |
of the expression of | 7 |
at days two and | 7 |
results suggest that the | 7 |
in contrast to the | 7 |
with pfu of rzikv | 7 |
more than of the | 7 |
can be used for | 7 |
and porcine epidemic diarrhea | 7 |
a novel coronavirus in | 7 |
expression of the viral | 7 |
in addition to the | 7 |
the identity of the | 7 |
compared to the control | 7 |
the replication of sars | 7 |
similar to those of | 7 |
cells in the absence | 7 |
titer was determined by | 7 |
a wide range of | 7 |
was carried out using | 7 |
in both cell types | 7 |
identification and characterization of | 7 |
as severe acute respiratory | 7 |
used to infect vero | 7 |
the neutralization capacity of | 7 |
coronavirus in patients with | 7 |
with acute respiratory distress | 7 |
wild type and ribv | 7 |
for min and then | 7 |
confluent monolayers of vero | 7 |
at nm was measured | 7 |
mode of action of | 7 |
the absence of trypsin | 7 |
higher than that in | 7 |
cell death during infection | 7 |
as well as in | 7 |
entry into host cells | 7 |
significant difference between the | 7 |
of these cell lines | 7 |
data are shown as | 7 |
during its propagation in | 7 |
monolayers of vero e | 7 |
was detected in the | 7 |
play an important role | 7 |
the antiviral efficacy of | 7 |
in severe acute respiratory | 7 |
in vero cells as | 7 |
were compared to those | 7 |
which is consistent with | 7 |
in both cell lines | 7 |
the percentage of infected | 7 |
viral titers in the | 7 |
to vero e cells | 7 |
in vero cells was | 7 |
moi of pfu cell | 7 |
associated with acute respiratory | 7 |
the g c mutation | 7 |
vero cells were pretreated | 7 |
new variants of porcine | 7 |
the effects of the | 7 |
with paraformaldehyde in pbs | 7 |
cell fusion activities of | 7 |
to test the invasion | 7 |
h before virus infection | 7 |
cells were then washed | 7 |
observed in vero cells | 7 |
analysis of the growth | 7 |
human airway epithelial cells | 7 |
committee on taxonomy of | 7 |
complete genome sequence of | 7 |
lines were infected with | 7 |
the presence of the | 7 |
reverse genetics system for | 7 |
of the intracellular extracts | 7 |
the absence of virus | 7 |
cells were resuspended in | 7 |
early stages of infection | 7 |
cells were seeded at | 7 |
in the spike protein | 7 |
then infected with pedv | 7 |
the early stage of | 7 |
in different cell lines | 7 |
alone or in combination | 7 |
pi k akt signaling | 7 |
plates were incubated at | 7 |
for vero e cells | 7 |
we found that the | 7 |
well plates at a | 7 |
titers were determined by | 7 |
ns a a v | 7 |
on the plasma membrane | 7 |
the s and s | 7 |
test was used to | 7 |
in vitro prophylactic and | 7 |
entry into vero cells | 7 |
the genome of the | 7 |
were used at a | 7 |
the viability of the | 7 |
vero e cell culture | 7 |
between the gds and | 7 |
vitro prophylactic and therapeutic | 7 |
in the cytoplasm of | 7 |
with uncleaved s protein | 7 |
no conflict of interest | 7 |
vitro prophylactic effects of | 7 |
the culture supernatants were | 7 |
the replication cycle of | 7 |
in this study were | 7 |
in the vero e | 7 |
of viral rna in | 7 |
supplemented with fbs and | 6 |
the nucleotide sequence of | 6 |
were infected with chikv | 6 |
the arg residue to | 6 |
experiment with two replicates | 6 |
incubated with gds or | 6 |
studies have suggested that | 6 |
sequence of the sars | 6 |
herpes simplex virus glycoprotein | 6 |
the etiological agent of | 6 |
of the virus was | 6 |
the authors declare no | 6 |
s and s subunits | 6 |
in vero cells with | 6 |
various time points post | 6 |
water was used as | 6 |
activity of the s | 6 |
in the coinfection group | 6 |
was used in the | 6 |
species severe acute respiratory | 6 |
total rna extracted from | 6 |
the species severe acute | 6 |
respiratory syndrome coronavirus infection | 6 |
tissue culture infectious dose | 6 |
at h after transfection | 6 |
the addition of trypsin | 6 |
antiviral activity against sars | 6 |
this is the first | 6 |
epidermal growth factor receptor | 6 |
the institutional animal care | 6 |
cov infection in vero | 6 |
infectious bronchitis virus a | 6 |
plays an important role | 6 |
sirna and infected with | 6 |
fit to a variable | 6 |
virus is also shown | 6 |
lungs and spinal cord | 6 |
approximately and in the | 6 |
performed as described previously | 6 |
both in vitro and | 6 |
activities of these s | 6 |
were removed by washing | 6 |
of these s constructs | 6 |
in the beaudette strain | 6 |
van dinten et al | 6 |
with an equal volume | 6 |
caused by a novel | 6 |
each well and incubated | 6 |
in an atmosphere of | 6 |
in vero cells but | 6 |
of the international committee | 6 |
in the viral genome | 6 |
samples were collected from | 6 |
entry into vero e | 6 |
western blot analysis of | 6 |
absence of virus is | 6 |
virus entry at the | 6 |
the viral life cycle | 6 |
three times with pbst | 6 |
virus titers in supernatants | 6 |
the genome sequence of | 6 |
were fixed at hpi | 6 |
cells were detected by | 6 |
binding and entry process | 6 |
were purchased from the | 6 |
involved in pedv entry | 6 |
of mx and isg | 6 |
the footpad with pfu | 6 |
a reverse genetics system | 6 |
times with pbs and | 6 |
of viral rna synthesis | 6 |
a new porcine enteric | 6 |
vero cells were transfected | 6 |
protein has been shown | 6 |
in vero cells is | 6 |
the results of this | 6 |
was also observed in | 6 |
j cells were pre | 6 |
inhibit the replication of | 6 |
that the vero e | 6 |
distilled water was used | 6 |
by the institutional animal | 6 |
to increasing concentrations of | 6 |
with paraformaldehyde for min | 6 |
of the virus and | 6 |
were performed in triplicate | 6 |
of the ns a | 6 |
vero e cells at | 6 |
reverse transcription was performed | 6 |
the mutant virus was | 6 |
of the severe acute | 6 |
in group were inoculated | 6 |
were mapped to the | 6 |
the treatment of zikv | 6 |
new porcine enteric coronavirus | 6 |
a potent inhibitor of | 6 |
hu carrying mutations in | 6 |
inhibition was determined relative | 6 |
p p p p | 6 |
respiratory syndrome coronavirus in | 6 |
cells exposed to increasing | 6 |
were carried out in | 6 |
of the virus life | 6 |
of virus is also | 6 |
fusion activities of these | 6 |
of the sars vaccine | 6 |
bars represent standard deviations | 6 |
to test this hypothesis | 6 |
experiments were performed in | 6 |
with the exception of | 6 |
at later time points | 6 |
footpad with pfu of | 6 |
two replicates is shown | 6 |
variable response curve from | 6 |
cell viability was measured | 6 |
of intracellular viral rna | 6 |
and cells were washed | 6 |
we did not observe | 6 |
cut fragment of p | 6 |
was significantly higher than | 6 |
stained with crystal violet | 6 |
in one of the | 6 |
transducer and activator of | 6 |
virus inoculum was removed | 6 |
with diarrhea in swine | 6 |
of the vero cells | 6 |
food and drug administration | 6 |
nucleotide sequence of the | 6 |
hepatitis c virus infection | 6 |
of the antiviral activity | 6 |
viability of vero cells | 6 |
passaged in vero cells | 6 |
analysis was performed using | 6 |
confirmed by western blotting | 6 |
from one representative experiment | 6 |
drugs in the absence | 6 |
did not affect the | 6 |
birds in each group | 6 |
of vero cells was | 6 |
levels of zikv rna | 6 |
percentage of infected cells | 6 |
a variable response curve | 6 |
were similar to those | 6 |
be involved in the | 6 |
our data indicated that | 6 |
rna and sg mrna | 6 |
human immunodeficiency virus type | 6 |
and incubated with gds | 6 |
cytotoxic effect on vero | 6 |
also been shown to | 6 |
for the presence of | 6 |
then incubated for h | 6 |
of at least three | 6 |
e cells infected with | 6 |
of avian infectious bronchitis | 6 |
isolation of a novel | 6 |
and infected with junv | 6 |
c for h then | 6 |
the three cell lines | 6 |
single amino acid substitution | 6 |
has been reported that | 6 |
of the two viruses | 6 |
were then incubated with | 6 |
like particle associated with | 6 |
after h of adsorption | 6 |
least three independent experiments | 6 |
against porcine epidemic diarrhea | 6 |
approved by the institutional | 6 |
infected cells treated with | 6 |
possessed an in vitro | 6 |
inhibition of viral rna | 6 |
by severe acute respiratory | 6 |
were orally inoculated with | 6 |
of hepatitis c virus | 6 |
replication of sabin poliovirus | 6 |
cells were maintained at | 6 |
of the parental virus | 6 |
of the cell lines | 6 |
cleavage of the s | 6 |
induces apoptosis in vero | 6 |
female balb c mice | 6 |
were approved by the | 6 |
immunostaining using the pan | 6 |
vero cell monolayers in | 6 |
total rna was isolated | 6 |
in the icpc a | 6 |
particle associated with diarrhea | 6 |
prrsv and pedv were | 6 |
the cell lysates were | 6 |
but there was no | 6 |
titrated by plaque assay | 6 |
the a v mutation | 6 |
all cell lines were | 6 |
exposed to increasing concentrations | 6 |
containing trypsin for h | 6 |
it has been reported | 6 |
to be able to | 6 |
virus in porcine cell | 6 |
titers in the supernatant | 6 |
in a volume of | 6 |
were infected with sars | 6 |
cdna clone of mv | 6 |
cells as well as | 6 |
for prrsv and pedv | 6 |
followed by cycles of | 6 |
cells were then incubated | 6 |
vero cells in the | 6 |
statistical analysis was performed | 6 |
increasing concentrations of drugs | 6 |
institute of infectious diseases | 6 |
fixed with paraformaldehyde for | 6 |
with a moi of | 6 |
has also been reported | 6 |
for h prior to | 6 |
a a v mutation | 6 |
a moi of for | 6 |
sequence analysis of the | 6 |
were treated with different | 6 |
clarified by centrifugation at | 6 |
at a dosage of | 6 |
isolation of porcine epidemic | 6 |
coronavirus spike protein by | 6 |
inhibits the replication of | 6 |
to be involved in | 6 |
and titrated by plaque | 6 |
with two replicates is | 6 |
from vero cells infected | 6 |
cells as described previously | 6 |
been shown to induce | 6 |
to a variable response | 6 |
the expression of a | 6 |
and the supernatants were | 6 |
body weight loss and | 6 |
introduced into vero cells | 6 |
the spike glycoprotein of | 6 |
terminal domain of the | 6 |
can be used to | 6 |
gds or gds strains | 6 |
our results showed that | 6 |
in cells treated with | 6 |
pcr was performed using | 6 |
the s domain of | 6 |
removed and the cells | 6 |
in the context of | 6 |
with or without blfp | 6 |
of drugs in the | 6 |
in the form of | 6 |
log s gene copies | 6 |
by western blot analysis | 6 |
response curve from one | 6 |
antiviral activity of the | 6 |
cells were fixed at | 6 |
has the potential to | 6 |
virus gd protein to | 6 |
s region of the | 6 |
of egfr pi k | 6 |
fragment of p tl | 6 |
from three independent experiments | 6 |
antiviral activity of potential | 6 |
of the mock group | 6 |
concentrations of peptide h | 6 |
were obtained from the | 6 |
were infected with zikv | 6 |
results confirmed that the | 6 |
signal transducer and activator | 6 |
expression levels of the | 6 |
dephosphorylation of stat in | 6 |
group were inoculated with | 6 |
is shown in figure | 6 |
moi of for h | 6 |
infected or infected with | 6 |
the presence of mm | 6 |
and activator of transcription | 6 |
for porcine epidemic diarrhea | 6 |
of a variety of | 6 |
cells infected with ribv | 6 |
for the generation of | 6 |
s rrna and rpoa | 6 |
the presence of a | 6 |
added to vero cells | 6 |
the blfp crude extract | 6 |
using graphpad prism software | 6 |
concentrations of drugs in | 6 |
isolates of sars coronavirus | 6 |
of the arg residue | 6 |
in vero cell culture | 6 |
in this study are | 6 |
was similar to that | 6 |
in dmem supplemented with | 6 |
p in vero cells | 6 |
s gene copies ml | 6 |
infected vero cells with | 6 |
the cells three times | 6 |
from cells transfected with | 6 |
cells were fixed in | 6 |
dmem supplemented with fbs | 6 |
mutation of the arg | 6 |
supernatants were harvested at | 6 |
that myricetin may interact | 6 |
there were no significant | 6 |
activity of peptide h | 6 |
conjugated to alexa fluor | 6 |
significant difference between groups | 6 |
the murine coronavirus spike | 6 |
did not show any | 6 |
be explained by the | 6 |
as early as hpi | 5 |
the fusion activity of | 5 |
different concentrations of each | 5 |
entry at the plasma | 5 |
the centers for disease | 5 |
followed by staining with | 5 |
mechanisms of action of | 5 |
effectively inhibit the recently | 5 |
which was significantly lower | 5 |
and chloroquine effectively inhibit | 5 |
as a receptor for | 5 |
peripheral blood mononuclear cells | 5 |
bands were visualized using | 5 |
after the second transfection | 5 |
is known about the | 5 |
the fact that the | 5 |
expression in vero cells | 5 |
of pfu per cell | 5 |
and clarified by centrifugation | 5 |
respiratory syndrome coronavirus replication | 5 |
cell lines were maintained | 5 |
plaque assay in vero | 5 |
by a novel coronavirus | 5 |
by plaque assay in | 5 |
in order to determine | 5 |
depends on ace and | 5 |
virus titers were determined | 5 |
signal transduction in sars | 5 |
entry depends on ace | 5 |
belongs to the genus | 5 |
diarrhea virus spike protein | 5 |
of mm ammonium chloride | 5 |
effects against pedv in | 5 |
the american type culture | 5 |
in vero and huh | 5 |
it was reported that | 5 |
it will be interesting | 5 |
the cells were treated | 5 |
to inoculate a cm | 5 |
nucleotides to of the | 5 |
pcr products were purified | 5 |
treatment of vero cells | 5 |
fig a and b | 5 |
epidemic diarrhea virus spike | 5 |
dmso was used as | 5 |
cell fusion activity by | 5 |
coronavirus induces apoptosis in | 5 |
the plaque reduction assay | 5 |
to the parental pedvpt | 5 |
we also found that | 5 |
of pigs with a | 5 |
were detected by immunocytochemistry | 5 |
cells pretreated with the | 5 |
of harringtonine for h | 5 |
were added to the | 5 |
viral rna mini kit | 5 |
in porcine cell cultures | 5 |
is lethal to the | 5 |
cells infected with sars | 5 |
a line of african | 5 |
to the cell surface | 5 |
viral titers were determined | 5 |
extracted from the infected | 5 |
data were analyzed by | 5 |
blotting at hpi and | 5 |
mutant virus from p | 5 |
of the mutant rzikv | 5 |
the lab isolates were | 5 |
at days after challenge | 5 |
to further confirm the | 5 |
infection of vero e | 5 |
the specificity of the | 5 |
were significantly more attenuated | 5 |
turkey with confirmed covid | 5 |
is blocked by a | 5 |
drugs were used at | 5 |
significance was analyzed from | 5 |
of action of ribavirin | 5 |
to the research community | 5 |
the viral envelope with | 5 |
the recombinant ibvs were | 5 |
cells treated with the | 5 |
and stained with anti | 5 |
in dmem containing fbs | 5 |
swine pedv positive serum | 5 |
sars coronavirus infection in | 5 |
as a loading control | 5 |
were fixed with formaldehyde | 5 |
analyzed by western blotting | 5 |
to cells and incubated | 5 |
strains were and in | 5 |
plaque assays were performed | 5 |
patient in turkey with | 5 |
signaling pathways are activated | 5 |
facilitate the development of | 5 |
for replication in cell | 5 |
and is blocked by | 5 |
or gds strains for | 5 |
natural science foundation of | 5 |
the s s region | 5 |
the data presented as | 5 |
on cell infection by | 5 |
after days of incubation | 5 |
chloroquine effectively inhibit the | 5 |
were introduced into vero | 5 |
previous studies have shown | 5 |
cell entry depends on | 5 |
blocks cell cycle progression | 5 |
of each viral stock | 5 |
by interfering with the | 5 |
a or icpc a | 5 |
used to determine the | 5 |
national institute of infectious | 5 |
read and approved the | 5 |
to the results of | 5 |
may be involved in | 5 |
were removed and the | 5 |
at each time point | 5 |
functions within the helicase | 5 |
treated and untreated cells | 5 |
via the cyclin d | 5 |
vero cells were co | 5 |
extracted from vero cells | 5 |
mem containing trypsin for | 5 |
pedv s protein was | 5 |
used to inoculate a | 5 |
cell substrates for the | 5 |
a better understanding of | 5 |
vero cells transfected with | 5 |
the mode of action | 5 |
a variety of viruses | 5 |
an moi of pfu | 5 |
followed by incubation with | 5 |
the most commonly used | 5 |
and approved the final | 5 |
cells treated with or | 5 |
on the left indicate | 5 |
in vitro assembly of | 5 |
the e glycoprotein of | 5 |
the number of cells | 5 |
and virus titers were | 5 |
phosphorylation and activation of | 5 |
expression of s protein | 5 |
the s rrna and | 5 |
piglets in group were | 5 |
were collected from the | 5 |
was analyzed from t | 5 |
data presented as mean | 5 |
was used to compare | 5 |
from the ministry of | 5 |
the infection of pedv | 5 |
as well as other | 5 |
of this ns a | 5 |
the wt sequence during | 5 |
during the course of | 5 |
plates were incubated for | 5 |
an atmosphere of co | 5 |
pcr was carried out | 5 |
wt sequence during its | 5 |
and the presence of | 5 |
of the subgenomic mrna | 5 |
of infected vero e | 5 |
of s gene mutants | 5 |
end of the genome | 5 |
up to days post | 5 |
the effect of trypsin | 5 |
cov in vero e | 5 |
cleavage of the e | 5 |
in vitro prophylactic effect | 5 |
the groups treated with | 5 |
that pedv entry followed | 5 |
binding site in the | 5 |
groups of five female | 5 |
dna and rna viruses | 5 |
and western blotting analysis | 5 |
vero e cells by | 5 |
were propagated in vero | 5 |
virus in cultured cells | 5 |
fixed at hpi and | 5 |
clinically proven protease inhibitor | 5 |
was allowed to proceed | 5 |
then washed three times | 5 |
of the s gene | 5 |
tissue culture supernatants were | 5 |
a patient in turkey | 5 |
the s subunit of | 5 |
infection of pigs with | 5 |
factors that influence ebola | 5 |
tmprss and is blocked | 5 |
to of the viral | 5 |
no significant differences in | 5 |
blfp crude extract were | 5 |
of transmissible gastroenteritis coronavirus | 5 |
viral titer in the | 5 |
and immunostaining using the | 5 |
treated with proteinase k | 5 |
allowed to proceed for | 5 |
the amount of virus | 5 |
than those of the | 5 |
vero e cells to | 5 |
supernatants were determined by | 5 |
we found that pedv | 5 |
cellulose in mem containing | 5 |
have been used to | 5 |
of the ibv genome | 5 |
in vitro transcribed rna | 5 |
diarrhea virus in porcine | 5 |
the culture of vero | 5 |
was considered to be | 5 |
serial dilutions of the | 5 |
particular ic value of | 5 |
were monitored daily for | 5 |
adapted ibv beaudette strain | 5 |
the recovery of infectious | 5 |
that influence ebola antiviral | 5 |
fold serial dilution of | 5 |
at day after infection | 5 |
together with the n | 5 |
h and incubated with | 5 |
the feasibility of generating | 5 |
was used to detect | 5 |
spike protein is a | 5 |
expressed as the mean | 5 |
possessed in vitro prophylactic | 5 |
the vero jcrb cell | 5 |
coronavirus associated with human | 5 |
time course analysis of | 5 |
a duplicated subgenomic promoter | 5 |
these results indicate that | 5 |
jejunum and ileum of | 5 |
c bl j mice | 5 |
the entire genome of | 5 |
in hek t cells | 5 |
linear fit to a | 5 |
ebola antiviral activities in | 5 |
of viral protein expression | 5 |
twice with sterile pbs | 5 |
on the cell surface | 5 |
member of the genus | 5 |
control cells containing the | 5 |
institute of biomedical sciences | 5 |
cell lines such as | 5 |
cotton rats in groups | 5 |
virus in vero cells | 5 |
did not observe a | 5 |
cycle progression at g | 5 |
the qiaamp viral rna | 5 |
it should be noted | 5 |
from a patient with | 5 |
with the n transcripts | 5 |
identity of the rescued | 5 |
of the parental pedvpt | 5 |
harvested at h post | 5 |
carried out in the | 5 |
stages of viral infection | 5 |
the recently emerged novel | 5 |
we were able to | 5 |
with respect to the | 5 |
of african swine fever | 5 |
as a positive control | 5 |
that the inhibition of | 5 |
apoptosis induced by sars | 5 |
compared with the zikv | 5 |
us porcine epidemic diarrhea | 5 |
with methylcellulose in dmem | 5 |
pfu ml at hpi | 5 |
to the method described | 5 |
the drug was added | 5 |
of the e glycoprotein | 5 |
confluent vero cells in | 5 |
science foundation of china | 5 |
these data indicate that | 5 |
its propagation in vero | 5 |
the membrane was incubated | 5 |
detection limit of the | 5 |
cdna clone of the | 5 |
supernatants were collected and | 5 |
the virus titer in | 5 |
the heptad repeat region | 5 |
significantly higher than that | 5 |
acidophilus cmul and l | 5 |
removed and the monolayers | 5 |
be attributed to the | 5 |
a wide variety of | 5 |
in turkey with confirmed | 5 |
treated with lm snap | 5 |
by grants from the | 5 |
vero cells were prepared | 5 |
the cyclin d prb | 5 |
treated with the recombinant | 5 |
conformational changes in the | 5 |
were found to be | 5 |
was determined by a | 5 |
cells but not in | 5 |
the treatment of covid | 5 |
qiaamp viral rna mini | 5 |
with increasing concentrations of | 5 |
into vero e cells | 5 |
order genetic assembly system | 5 |
to determine whether the | 5 |
provided in supplementary file | 5 |
the seven recombinant ibvs | 5 |
assay was performed to | 5 |
was used at a | 5 |
with a new porcine | 5 |
we are grateful to | 5 |
mutation in a domain | 5 |
to further investigate the | 5 |
a clinically proven protease | 5 |
standard deviations of the | 5 |
in a line of | 5 |
of the bacterial intracellular | 5 |
was measured using a | 5 |
growth properties of the | 5 |
supported by grants from | 5 |
plates at a density | 5 |
were incubated for min | 5 |
and tmprss and is | 5 |
for h followed by | 5 |
hsv binding and entry | 5 |
numbers on the left | 5 |
drugs for treatment of | 5 |
icpc a or icpc | 5 |
cellular egfr pi k | 5 |
of an antisense oligonucleotide | 5 |
infectious bronchitis virus in | 5 |
the infectious bronchitis virus | 5 |
the infected vero cells | 5 |
e glycoprotein of murine | 5 |
h prior to infection | 5 |
progression at g g | 5 |
vero cells pretreated with | 5 |
vero e and llcmk | 5 |
our understanding of the | 5 |
the particular ic value | 5 |
results were confirmed by | 5 |
were used to infect | 5 |
of an avian coronavirus | 5 |
of human coronavirus e | 5 |
cyclin d prb pathway | 5 |
at room temperature with | 5 |
pigs with a new | 5 |
antiviral activities in cell | 5 |
from the american type | 5 |
protein to interfere with | 5 |
are listed in table | 5 |
of icpc a was | 5 |
the viral rna was | 5 |
could be due to | 5 |
at rt for h | 5 |
the bacterial intracellular extracts | 5 |
patients recovered from covid | 5 |
the bac cdna clone | 5 |
at room temperature and | 5 |
the generation of a | 5 |
and u cell lines | 5 |
inhibit the recently emerged | 5 |
and naming it sars | 5 |
genomic characterization of a | 5 |
of the infected cells | 5 |
when the drug was | 5 |
concentrations of each compound | 5 |
significant difference in the | 5 |
the activation of egfr | 5 |
was approved by the | 5 |
all animal experiments were | 5 |
little is known about | 5 |
of the viral life | 5 |
further characterization of the | 5 |
at this time point | 5 |
transcripts containing the g | 5 |
antiviral activity in vitro | 5 |
antiviral drug candidates against | 5 |
were then overlaid with | 5 |
the viability of vero | 5 |
the mechanism of action | 5 |
and then shifted to | 5 |
nucleotide sequences of the | 5 |
stocks were prepared by | 5 |
a broad range of | 5 |
of west nile virus | 5 |
the detection of icpc | 5 |
resuspended in ml of | 5 |
results were expressed as | 5 |
the detection of the | 5 |
higher than those of | 5 |
with porcine epidemic diarrhea | 5 |
was added at the | 5 |
of the vero cell | 5 |
in a similar manner | 5 |
was carried out with | 5 |
with the zikv group | 5 |
weight loss and survival | 5 |
cells infected with chikv | 5 |
human respiratory disease in | 5 |
recently emerged novel coronavirus | 5 |
ace and tmprss and | 5 |
the entry mechanism of | 5 |
in vitro inhibition of | 5 |
well and incubated at | 5 |
western blotting at hpi | 5 |
demonstrate the feasibility of | 5 |
added to cells and | 5 |
infected with pedv at | 5 |
the egfp reporter gene | 5 |
in the pathogenesis of | 5 |
in accordance with the | 5 |
and foetal calf serum | 5 |
and hepatitis c virus | 5 |
invasion rates of pedv | 5 |
gd protein to interfere | 5 |
allowed to incubate for | 5 |
in mem containing trypsin | 5 |
removed and cells were | 5 |
was not observed in | 5 |
of a number of | 5 |
swine acute diarrhea syndrome | 5 |
into the host cell | 5 |
inoculated with ml of | 5 |
these results confirm that | 5 |
h after the first | 5 |
of the cell monolayer | 5 |
the vero cell monolayers | 5 |
remdesivir and chloroquine effectively | 5 |
the consensus sequence of | 5 |
adsorption and membrane fusion | 5 |
were incubated at room | 5 |
methyl cellulose in mem | 5 |
treatment of cells with | 5 |
assay was performed as | 5 |
a high degree of | 5 |
an essential role in | 5 |
approved for clinical use | 5 |
plaque assay and immunostaining | 5 |
in cells electroporated with | 5 |
in a domain with | 5 |
the plasma membrane of | 5 |
the pcr products were | 5 |
of virus to the | 5 |
stock was determined by | 5 |
will be interesting to | 5 |
and incubated for min | 5 |
groups treated with the | 5 |
drug candidates against sars | 5 |
tcid ml of the | 5 |
new coronavirus associated with | 5 |
log rna copies ml | 5 |
at different stages of | 5 |
the oxygen atom of | 5 |
after the first transfection | 5 |
the pedv blfp group | 5 |
gds strains for h | 5 |
those of the parental | 5 |
rrna and rpoa gene | 5 |
were treated with or | 5 |
myricetin may block hsv | 5 |
the sialic acid binding | 5 |
be used for the | 5 |
were fixed with acetone | 5 |
the parental vaccine strain | 5 |
these results demonstrate the | 5 |
pedv entry into vero | 5 |
substrates for the production | 5 |
were analyzed using the | 5 |
error bars represent the | 5 |
and mechanism of action | 5 |
were added to each | 5 |
effect of trypsin on | 5 |
rna was detected in | 5 |
of middle east respiratory | 5 |
were and in the | 5 |
with human respiratory disease | 5 |
authors declare no conflict | 5 |
were added to cells | 5 |
in the perinuclear region | 5 |
between the two cell | 5 |
vero cells as described | 5 |
influence ebola antiviral activities | 5 |
of the pedv s | 5 |
statistical significance was analyzed | 5 |
paraformaldehyde in pbs for | 5 |
cell lines for the | 5 |
in vitro models of | 5 |
the difference in the | 5 |
virus infection in vitro | 5 |
was kindly provided by | 5 |
the induction of cell | 5 |
wild type and mutant | 5 |
the cell monolayer was | 5 |
morbidity and mortality rates | 5 |
declare no conflict of | 5 |
the molecular biology of | 5 |
sam binding site in | 5 |
in the pedv blfp | 5 |
for h and incubated | 5 |
at g g phase | 5 |
with various concentrations of | 5 |
blocked by a clinically | 5 |
the number of foci | 5 |
study of the antiviral | 5 |
results of this study | 5 |
the icpc a and | 5 |
and the virus titer | 5 |
was washed three times | 5 |
in a co incubator | 5 |
the virus titer was | 5 |
g c mutant transcripts | 5 |
on ace and tmprss | 5 |
assay and immunostaining using | 5 |
commercially available antiviral agents | 5 |
the ministry of health | 5 |
in different cell types | 5 |
significantly higher than those | 5 |
infected with the recombinant | 5 |
was removed and cells | 5 |
test compared with the | 5 |
it is known that | 5 |
as the primary antibody | 5 |
in order to evaluate | 5 |
was carried out in | 5 |
and rpoa gene sequence | 5 |
sequence during its propagation | 5 |
were found in the | 5 |
peptide h and pedv | 5 |
would like to thank | 5 |
is a receptor for | 5 |
or absence of ribavirin | 5 |
detection of icpc a | 5 |
added into each well | 5 |
on the basis of | 5 |
were no significant differences | 5 |
to the end of | 5 |
cultured in the presence | 5 |
infection of pedv icpc | 5 |
gds strains were and | 5 |
the clinical management of | 5 |
infected with the mutant | 5 |
jnk and pi k | 5 |
infectivity of the in | 5 |
time points post infection | 5 |
according to the method | 5 |
porcine transmissible gastroenteritis virus | 5 |
replication in cell culture | 5 |
virus into vero cells | 5 |
associated with human respiratory | 5 |
and ck ch ldl | 5 |
the rescued virus was | 5 |
these s constructs are | 5 |
a new coronavirus associated | 5 |
and vero cell lines | 5 |
crude extract were determined | 5 |
or without blfp crude | 5 |
performed as described above | 5 |
of porcine reproductive and | 5 |
after washing twice with | 5 |
were cultured overnight in | 5 |
and the plaques were | 5 |
are thought to be | 5 |
ncov and naming it | 5 |
were separated by sds | 5 |
might be due to | 5 |
and the data presented | 5 |
was added to vero | 5 |
respiratory disease in china | 5 |
by a clinically proven | 5 |
in the heptad repeat | 5 |
and swine pedv positive | 5 |
difference between the gds | 4 |
the hpi time point | 4 |
porcine epidemic diarrhea coronavirus | 4 |
treated with various concentrations | 4 |
in mammalian cell lines | 4 |
is a class i | 4 |
for the study of | 4 |
avian infectious bronchitis coronavirus | 4 |
terminal region of the | 4 |
results indicated that pedv | 4 |
r k and r | 4 |
inoculum was removed followed | 4 |
the amount of gd | 4 |
for h in the | 4 |
fusion and syncytia formation | 4 |
a one sample t | 4 |
vero cell monolayers were | 4 |
cell cultures and mice | 4 |
step growth curves of | 4 |
respiratory syncytial virus infection | 4 |
new coronavirus of probable | 4 |
in the release of | 4 |
a new coronavirus of | 4 |
coronavirus of probable bat | 4 |
the expression of s | 4 |
icpc a replicated to | 4 |
the different cell lines | 4 |
of vero cells vol | 4 |
detection and quantification of | 4 |
and hela cell lines | 4 |
that the differences between | 4 |
grew to high titer | 4 |
at c followed by | 4 |
diarrhea virus into vero | 4 |
results demonstrate that the | 4 |
control by the humoral | 4 |
k akt signaling pathway | 4 |
amplification of cdna ends | 4 |
virions interacted with vero | 4 |
from a vero cell | 4 |
play important roles in | 4 |
mapk and its downstream | 4 |
in this report we | 4 |
murine coronavirus spike protein | 4 |
onset of clinical signs | 4 |
for min to remove | 4 |
to the s protein | 4 |
the percentage inhibition was | 4 |
for the clinical management | 4 |
lethal to the coronavirus | 4 |
transfected with the mutant | 4 |
seeded with vero e | 4 |
reduction of the viral | 4 |
vero cells were incubated | 4 |
cell spread of hsv | 4 |
the reason for the | 4 |
newly discovered coronavirus associated | 4 |
an increase in the | 4 |
with prrsv or pedv | 4 |
porcine aminopeptidase n is | 4 |
figure a and b | 4 |
or in combination with | 4 |
coronavirus of bat origin | 4 |
that the intracellular extracts | 4 |
inhibitory effects against pedv | 4 |
prevention and control of | 4 |
efficacy of m f | 4 |
percentage inhibition was determined | 4 |
outbreak associated with a | 4 |
data were analyzed using | 4 |
the type i interferon | 4 |
viral replication in the | 4 |
u ml penicillin and | 4 |
based on the sequence | 4 |
prepared by freezing thawing | 4 |
representative of three separate | 4 |
with an excess of | 4 |
on ice for min | 4 |
human suspension cell lines | 4 |
to infection by sars | 4 |
at the amino acid | 4 |
during the early stages | 4 |
a man with pneumonia | 4 |
protein was detected by | 4 |
characterization of a newly | 4 |
replication of prrsv and | 4 |
that pedv enters vero | 4 |
using the mmessage mmachine | 4 |
the central nervous system | 4 |
used in the pcr | 4 |
coronavirus severe acute respiratory | 4 |
activity of toremifene citrate | 4 |
as early as h | 4 |
analyzed by western blot | 4 |
with error bars represent | 4 |
in vero cells by | 4 |
with pedv at moi | 4 |
is due to the | 4 |
pedv entry relies on | 4 |
and added to the | 4 |
moi of pfu per | 4 |
the number of viral | 4 |
at concentrations ranging from | 4 |
when data are compared | 4 |
of the small intestine | 4 |
vero cells in a | 4 |
the time of infection | 4 |
with sabin poliovirus type | 4 |
by the world health | 4 |
viral control by the | 4 |
important role in the | 4 |
than the gds strain | 4 |
the presence of rounded | 4 |
to the best of | 4 |
p mapk and its | 4 |
attenuated in vitro and | 4 |
its interaction with the | 4 |
associated with porcine epidemic | 4 |
species as well as | 4 |
identification of antiviral drug | 4 |
d the vero cells | 4 |
similar results were obtained | 4 |
petrus jansen van vuren | 4 |
by the humoral immune | 4 |
the selection of the | 4 |
from a man with | 4 |
entire genome of each | 4 |
values are representative of | 4 |
myricetin may be able | 4 |
it is necessary to | 4 |
fold dilutions of the | 4 |
in the vero and | 4 |
the result shown is | 4 |
mixture of two viruses | 4 |
is a major receptor | 4 |
biology and pathogenesis of | 4 |
and isg genes in | 4 |
were used as a | 4 |
were incubated with rabbit | 4 |
the large polymerase protein | 4 |
to this article can | 4 |
emergence of resistant viruses | 4 |
time of infection or | 4 |
knockdown of the expression | 4 |
detected in vero cells | 4 |
in human lung cells | 4 |
entry mechanism of pedv | 4 |
in the s s | 4 |
the culture supernatant was | 4 |
those of the mock | 4 |
do not appear to | 4 |
targeting the n gene | 4 |
related coronavirus of bat | 4 |
the genetic stability of | 4 |
did not exhibit any | 4 |
sabin poliovirus type in | 4 |
to the fact that | 4 |
simplex virus type infection | 4 |
antigens targeting the s | 4 |
replication in vero cells | 4 |
are statistically significant when | 4 |
represent standard deviations of | 4 |
effects of chloroquine on | 4 |
this article can be | 4 |
that some of the | 4 |
electroporated into vero cells | 4 |
more insight into the | 4 |
both icpc a and | 4 |
to investigate the effect | 4 |
inoculated with ped virus | 4 |
a betacoronavirus in group | 4 |
were more susceptible to | 4 |
derived vero cell line | 4 |
data are expressed as | 4 |
to the coronavirus infectious | 4 |
pedv enters vero cells | 4 |
sequence derived from the | 4 |
vero cells were first | 4 |
virus titer was determined | 4 |
duration of the experiment | 4 |
the peak viral titer | 4 |
like particles associated with | 4 |
type and mutant viruses | 4 |
amount of gd protein | 4 |
member of the family | 4 |
the use of a | 4 |
on the consensus sequence | 4 |
in vero jcrb cells | 4 |
constitutive activation of stat | 4 |
effect of ribavirin on | 4 |
amount of viral rna | 4 |
the phosphorylation of egfr | 4 |
duplicated subgenomic promoter and | 4 |
located in the c | 4 |
crude extract of blfp | 4 |
pellet was resuspended in | 4 |
a bacterial artificial chromosome | 4 |
rna was extracted and | 4 |
were conducted based on | 4 |
with the seven recombinant | 4 |
as a percentage of | 4 |
active in vero cells | 4 |
cells were lysed with | 4 |
apoptosis and necrosis have | 4 |
frames encode nonessential functions | 4 |
number of asterisks indicate | 4 |
on porcine nidovirus replication | 4 |
reading frames encode nonessential | 4 |
were assessed with a | 4 |
for severe acute respiratory | 4 |
inhibited at a concentration | 4 |
pedv rna was detected | 4 |
at and h post | 4 |
produced by escherichia coli | 4 |
the differentially expressed proteins | 4 |
intranasal and ocular routes | 4 |
with phosphate buffered saline | 4 |
based on the consensus | 4 |
to the negative control | 4 |
signaling pathways in sars | 4 |
assess the antiviral activity | 4 |
gene was amplified by | 4 |
adsorbed to hela cells | 4 |
both apoptosis and necrosis | 4 |
blfp as a feed | 4 |
the total amount of | 4 |
was used in all | 4 |
is a potent inhibitor | 4 |
the development of new | 4 |
the polybasic cleavage motif | 4 |
titer in vero cells | 4 |
each well and the | 4 |
s protein by trypsin | 4 |
virus produced in c | 4 |
indicate that the differences | 4 |
of p mapk and | 4 |
compared the effects of | 4 |
virus strains in the | 4 |
as a feed additive | 4 |
of influenza a viruses | 4 |
and extracellular supernatants of | 4 |
through direct interaction with | 4 |
were treated with proteinase | 4 |
the small intestine of | 4 |
by the duplex rt | 4 |
protection against challenge with | 4 |
for the sars coronavirus | 4 |
are expressed as the | 4 |
to evaluate the viral | 4 |
receptor for the sars | 4 |
vero cells have been | 4 |
cme to enter cells | 4 |
has also been shown | 4 |
the production of vaccines | 4 |
conducted based on three | 4 |
vero cells could be | 4 |
well plates were inoculated | 4 |
a major receptor for | 4 |
with virus adsorption and | 4 |
have been deposited in | 4 |
well plates were washed | 4 |
higher than that of | 4 |
heptad repeat region of | 4 |
in the nearby clusters | 4 |
asterisks indicate that the | 4 |
caused by an hku | 4 |
cell death and survival | 4 |
not human coronavirus nl | 4 |
addition of guanosine to | 4 |
for the recombinant ibvs | 4 |
bars represent the mean | 4 |
on the viability of | 4 |
shown to induce apoptosis | 4 |
interaction with virus gd | 4 |
arg residue to a | 4 |
used as the primary | 4 |
of this graph is | 4 |
has not yet been | 4 |
nonstructural protein ns a | 4 |
to block viral entry | 4 |
nucleocapsid protein of sars | 4 |
s s region of | 4 |
differences were considered significant | 4 |
analyzed by flow cytometry | 4 |
man with pneumonia in | 4 |
in virus growth medium | 4 |
are shown as m | 4 |
in virus entry and | 4 |
in or near the | 4 |
for the prevention of | 4 |
the importance of this | 4 |
jev production in huh | 4 |
to replicate in these | 4 |
hepatitis virus strain a | 4 |
and middle east respiratory | 4 |
design of the study | 4 |
respiratory distress syndrome in | 4 |
infection with chlamydia abortus | 4 |
at c for min | 4 |
binding of gd to | 4 |
k and r l | 4 |
revealed that pedv entry | 4 |
passages in vero cells | 4 |
in vitro using the | 4 |
a final concentration of | 4 |
viral stocks were prepared | 4 |
entry step of sars | 4 |
and used to infect | 4 |
is possible that the | 4 |
kit according to the | 4 |
with a lethal dose | 4 |
of the family coronaviridae | 4 |
asterisks indicate significant difference | 4 |
we compared the effects | 4 |
productivity and susceptibility to | 4 |
allergy and infectious diseases | 4 |
at the beginning of | 4 |
to determine the effect | 4 |
the sequence of the | 4 |
can enter cells through | 4 |
to further assess the | 4 |
cells were then overlaid | 4 |
representative of the mean | 4 |
an increase in virus | 4 |
in blocking buffer for | 4 |
mmessage mmachine t kit | 4 |
not appear to be | 4 |
cm tissue culture flask | 4 |
against the pedv s | 4 |
cell lines can support | 4 |
reversion of the mutant | 4 |
with the mutant virus | 4 |
interacted with vero cells | 4 |
mm nh cl or | 4 |
after washing with pbs | 4 |
and characterization of the | 4 |
was performed according to | 4 |
mutation at nucleotide position | 4 |
the ministry of education | 4 |
mixed infections with chlamydia | 4 |
assay for detection and | 4 |
can be found in | 4 |
diarrhea virus strain pc | 4 |
that acquisition of the | 4 |
performed to determine the | 4 |
membrane fusion and reduces | 4 |
the causative agent of | 4 |
and monitored for cpe | 4 |
observed in cells infected | 4 |
expression of viral proteins | 4 |
was detected by qrt | 4 |
and used to inoculate | 4 |
statistical significance was determined | 4 |
point mutation in the | 4 |
we next sought to | 4 |
a positively charged amino | 4 |
shown is a representative | 4 |
of the number of | 4 |
targets of p mapk | 4 |
of ck ch ldl | 4 |
the mean from three | 4 |
positively charged amino acid | 4 |
vero e cells was | 4 |
due to the low | 4 |
the potential emergence of | 4 |
a of the coronavirus | 4 |
to further evaluate the | 4 |
and the overexpression of | 4 |
landscape of vero cells | 4 |
to ensure that the | 4 |
human and animal cells | 4 |
were aligned using the | 4 |
in vivo compared to | 4 |
characterization of severe acute | 4 |
virus to the cells | 4 |
below the detection limit | 4 |
by deka et al | 4 |
or near the catalytic | 4 |
the inhibitory activities of | 4 |
g for min to | 4 |
and the lack of | 4 |
plaques were visualized by | 4 |
in the spike glycoprotein | 4 |
scrofa and chlorocebus sabaeus | 4 |
mice infected with pfu | 4 |
detection and titration of | 4 |
cells and incubated for | 4 |
activity against both hsv | 4 |
in cultured cells and | 4 |
vero cells via clathrin | 4 |
for the duration of | 4 |
prior to incubation with | 4 |
most commonly used cell | 4 |
as a measure of | 4 |
in multiple cell types | 4 |
determine the effect of | 4 |
travel history to iran | 4 |
using the unpaired t | 4 |
the replication of porcine | 4 |
are summarized in table | 4 |
the most effective inhibitor | 4 |
been tested in humans | 4 |
observed in cells transfected | 4 |
the expression level of | 4 |
these two cell lines | 4 |
from the first patient | 4 |
cells transfected with z | 4 |
coronavirus spike protein for | 4 |
for the use of | 4 |
spike protein for membrane | 4 |
which belongs to the | 4 |
was removed followed by | 4 |
for membrane fusion and | 4 |
on sv in tissue | 4 |
the addition of guanosine | 4 |
for the ratio of | 4 |
absorbance at nm was | 4 |
supplemented with fetal calf | 4 |
was cloned by three | 4 |
a discontinuous transcription mechanism | 4 |
length transcripts containing the | 4 |
plaque assay on vero | 4 |
detected as early as | 4 |
infected with a moi | 4 |
have shown that the | 4 |
the virus passage virus | 4 |
activity of s protein | 4 |
the unpaired t test | 4 |
of cell death in | 4 |
strain was more sensitive | 4 |
were reported to have | 4 |
the limit of detection | 4 |
coronavirus replication in cell | 4 |
the morbidity and mortality | 4 |
at the institute of | 4 |
that vero e cells | 4 |
coronavirus in vero cells | 4 |
the cells were harvested | 4 |
were washed with phosphate | 4 |
were grown in dmem | 4 |
proteins were separated by | 4 |
enters vero cells via | 4 |
in the groups treated | 4 |
related to the sars | 4 |
cell lines infected with | 4 |
rapid amplification of cdna | 4 |
chikv ls and ls | 4 |
were used in this | 4 |
cells was determined using | 4 |
a role in the | 4 |
in both lungs and | 4 |
necrosis have been observed | 4 |
fold diluted virus stock | 4 |
for the staining of | 4 |
and found that the | 4 |
titers in supernatants were | 4 |
coronavirus associated with acute | 4 |
of the amino acid | 4 |
genetic stability of the | 4 |
viral rna in the | 4 |
calculated and expressed as | 4 |
emergent porcine epidemic diarrhea | 4 |
domain with undefined functions | 4 |
were incubated for days | 4 |
assay on vero cells | 4 |
enhancement of the cell | 4 |
for the induction of | 4 |
of infected cells was | 4 |
cases of severe acute | 4 |
coronavirus mouse hepatitis virus | 4 |
of lactic acid bacteria | 4 |
that the replication of | 4 |
nonessential functions for replication | 4 |
a pneumonia outbreak associated | 4 |
after the addition of | 4 |
and activation of p | 4 |
was able to induce | 4 |
before washing with pbs | 4 |
major receptor for the | 4 |
of the m protein | 4 |
based on the use | 4 |
when it was added | 4 |
the other cell lines | 4 |
and cells were incubated | 4 |
for h and were | 4 |
isolation in cell culture | 4 |
the correlation between the | 4 |
of antiviral activity of | 4 |
the ability of the | 4 |
infectious bronchitis virus is | 4 |
the coronavirus spike protein | 4 |
that the recombinant ibvs | 4 |
infectious virus from the | 4 |
virus strain pc a | 4 |
the production of virions | 4 |
successful entry into cells | 4 |
were washed once with | 4 |
a cm tissue culture | 4 |
tissue culture infective dose | 4 |
genotyping of emergent porcine | 4 |
by means of the | 4 |
in the s gene | 4 |
infection was allowed to | 4 |
cells were then infected | 4 |
pneumonia in saudi arabia | 4 |
replication in cell cultures | 4 |
of the novel human | 4 |
were intranasally inoculated with | 4 |
antiviral activity of ata | 4 |
cultured in vero cells | 4 |
prophylactic effects of the | 4 |
authors read and approved | 4 |
mesenteroides ypk against pedv | 4 |
vero jcrb cell line | 4 |
respiratory syndrome coronavirus entry | 4 |
was significantly inhibited by | 4 |
was quantified by real | 4 |
could be inhibited by | 4 |
for human coronavirus e | 4 |
e cells were treated | 4 |
in balb c mice | 4 |
were significantly lower than | 4 |
cathepsin l to infect | 4 |
the number of infected | 4 |
washed for three times | 4 |
on the replication of | 4 |
strain of porcine epidemic | 4 |
the s protein gene | 4 |
of infectious viral progeny | 4 |
samples was determined by | 4 |
the mutant virus from | 4 |
compared using the unpaired | 4 |
calpain and cathepsin inhibitors | 4 |
culture of vero e | 4 |
all of them survived | 4 |
important insights into the | 4 |
an inverted fluorescence microscope | 4 |
incubated for min at | 4 |
were approximately and in | 4 |
the method described by | 4 |
the igg and igm | 4 |
the species rhinolophus bat | 4 |
a percentage of the | 4 |
cell culture supernatants were | 4 |
of the mean from | 4 |
the duration of the | 4 |
but not human coronavirus | 4 |
late stage of zikv | 4 |
their effects on the | 4 |
to induce apoptosis in | 4 |
the s protein on | 4 |
the mmessage mmachine t | 4 |
which suggested that the | 4 |
incubated for h with | 4 |
guanosine to the ribavirin | 4 |
obtained from the american | 4 |
apoptosis via a caspase | 4 |
and iu ml for | 4 |
the concentration of the | 4 |
was supported by grants | 4 |
with pneumonia in saudi | 4 |
of the plasma membrane | 4 |
the internalization rates of | 4 |
protein similar to p | 4 |
of the synthetic viruses | 4 |
of the negative strand | 4 |
of stat in sars | 4 |
has been reported to | 4 |
strains in the united | 4 |
membrane fusion activity of | 4 |
for the propagation of | 4 |
the phosphorylation and activation | 4 |
both levels of zikv | 4 |
to the presence of | 4 |
between virus and host | 4 |
then the amount of | 4 |
with the plasma membrane | 4 |
of the antisense oligonucleotide | 4 |
visualized by staining with | 4 |
viral protein expression in | 4 |
encode nonessential functions for | 4 |
sv in tissue culture | 4 |
viruses at a multiplicity | 4 |
in agreement with these | 4 |
pedv entry followed clathrin | 4 |
and analyzed by flow | 4 |
and human leukocyte interferon | 4 |
gds strain was more | 4 |
it was observed that | 4 |
in vero cells for | 4 |
the second passage of | 4 |
vitro prophylactic effect against | 4 |
in the blfp crude | 4 |
the enzymatic activities of | 4 |
carried out using the | 4 |
that of the single | 4 |
either mm nh cl | 4 |
then washed with pbs | 4 |
mouse hepatitis virus strain | 4 |
genomic and subgenomic rna | 4 |
type i interferon in | 4 |
prophylactic effect against pedv | 4 |
respiratory syndrome coronavirus by | 4 |
the genomic and subgenomic | 4 |
entry of mers pseudovirions | 4 |
by western blot with | 4 |
virus binding and entry | 4 |
on the use of | 4 |
pck and cho cells | 4 |
of antiviral drug candidates | 4 |
room temperature for min | 4 |
bronchitis virus a polyprotein | 4 |
may be related to | 4 |
plays important roles in | 4 |
with fetal calf serum | 4 |
was performed using a | 4 |
staining with crystal violet | 4 |
samples were incubated for | 4 |
zika virus infection in | 4 |
to hepatitis c virus | 4 |
sus scrofa and chlorocebus | 4 |
porcine intestinal epithelial cells | 4 |
to further analyze the | 4 |
all authors read and | 4 |
associated with a new | 4 |
to the ribavirin treatment | 4 |
were then washed with | 4 |
of wild type and | 4 |
used in the treatment | 4 |
in order to test | 4 |
mice infected with the | 4 |
of guanosine to the | 4 |
treatment of vero e | 4 |
value of this graph | 4 |
of probable bat origin | 4 |
the percentage of ebov | 4 |
used for the production | 4 |
polyclonal antibody to the | 4 |
cells were cultured overnight | 4 |
pneumonia outbreak associated with | 4 |
of the african green | 4 |
simplex virus glycoprotein d | 4 |
at the same position | 4 |
infected with junv at | 4 |
were randomly assigned to | 4 |
virus infection and the | 4 |
was assigned values of | 4 |
been shown to have | 4 |
acute respiratory syndrome a | 4 |
the development of vaccine | 4 |
and hamster cell lines | 4 |
could be attributed to | 4 |
and pi k akt | 4 |
the activity of cyclin | 4 |
the outer capsid layer | 4 |
removed followed by three | 4 |
the infectivity of ibv | 4 |
in vitro inhibitory activity | 4 |
inhibitory activity of peptide | 4 |
were performed using the | 4 |
no significant difference in | 4 |
by staining with crystal | 4 |
of the s subunit | 4 |
transmission of zika virus | 4 |
japanese encephalitis reporter virus | 4 |
the cells were stained | 4 |
inhibitor of chikv replication | 4 |
both lungs and spinal | 4 |
plates were inoculated with | 4 |
that the compound may | 4 |
and then permeabilized with | 4 |
no and peroxynitrite on | 4 |
monolayer of vero e | 4 |
induced cell death than | 4 |
the humoral immune response | 4 |
pensaert and de bouck | 4 |
were inoculated with pfu | 4 |
statistically significant when data | 4 |
were used to amplify | 4 |
was effective against chikv | 4 |
and approved by the | 4 |
in the cell culture | 4 |
viral titer was determined | 4 |
observed for other alphaviruses | 4 |
cells were compared to | 4 |
changes in the viral | 4 |
cell culture model of | 4 |
cell fusion and syncytia | 4 |
containing the g c | 4 |
it has been shown | 4 |
approved the final manuscript | 4 |
the nucleocapsid protein of | 4 |
higher cell metabolic activity | 4 |
with a new coronavirus | 4 |
a part of the | 4 |
viral protein expression was | 4 |
from cells treated with | 4 |
was used to identify | 4 |
were fixed with methanol | 4 |
and the development of | 4 |
that the isolated virus | 4 |
error of the mean | 4 |
of virus yield inhibition | 4 |
of emergent porcine epidemic | 4 |
a result of the | 4 |
which recognizes residues to | 4 |
and infected with the | 4 |
with the addition of | 4 |
been used to treat | 4 |
belong to the species | 4 |
the majority of the | 4 |
isg genes in vero | 4 |
buffer for h at | 4 |
probably due to the | 4 |
passaged sabin poliovirus type | 4 |
the fact that they | 4 |
are available upon request | 4 |
cells for h at | 4 |
was shown to be | 4 |
in supernatants were determined | 4 |
g g phase via | 4 |
of a newly discovered | 4 |
death during infection by | 4 |
challenge with the parental | 4 |
in all groups were | 4 |
authors declare that there | 4 |
fusion and reduces viral | 4 |
virus adsorption and membrane | 4 |
swollen head syndrome in | 4 |
to vero cell monolayers | 4 |
enhanced the infectivity of | 4 |
the avian coronavirus infectious | 4 |
distress syndrome in humans | 4 |
are compared using the | 4 |
the plaques were counted | 4 |
region of the s | 4 |
in the ns a | 4 |
a crucial role in | 4 |
of prrsv or pedv | 4 |
with undefined functions within | 4 |
the ck ch ldl | 4 |
utilizes cathepsin l to | 4 |
bronchitis virus in cultured | 4 |
a domain with undefined | 4 |
to various types of | 4 |
used as a template | 4 |
we found that a | 4 |
and the number of | 4 |
the effects of chloroquine | 4 |
genome landscape of vero | 4 |
the vero cell culture | 4 |
result shown is a | 4 |
the mixture of two | 4 |
of vero cells is | 4 |
later stages of infection | 4 |
of the genome of | 4 |
based on three replicates | 4 |
preliminary step for cancer | 4 |
and genotyping of emergent | 4 |
and those less than | 4 |
the upper and lower | 4 |
a region of the | 4 |
were incubated at c | 4 |
s domain of mers | 4 |
indicate significant difference between | 4 |
compared to infected cells | 4 |
taiwan porcine epidemic diarrhea | 4 |
the number of plaques | 4 |
pellets were resuspended in | 4 |
the n transcripts were | 4 |
inoculate a cm tissue | 4 |
was reported to be | 4 |
downstream targets of p | 4 |
have been observed in | 4 |
the s protein was | 4 |
we set out to | 4 |
grown on glass coverslips | 4 |
with the mutant transcripts | 4 |
lungs were collected for | 4 |
antibody conjugated to alexa | 4 |
as shown in table | 4 |
g a grew to | 4 |
were added into each | 4 |
swine acute diarrhoea syndrome | 4 |
were harvested at h | 4 |
in a mouse model | 4 |
for h before infection | 4 |
inactivated fetal bovine serum | 4 |
adsorbed but not internalized | 4 |
the novel human betacoronavirus | 4 |
vero cells were pre | 4 |
calculated based on the | 4 |
the genome landscape of | 4 |
a newly discovered coronavirus | 4 |
virus titers were calculated | 4 |
s and s species | 4 |
is a representative of | 4 |
the nucleotide sequences of | 4 |
were prepared by freezing | 4 |
of pedv n proteins | 4 |
the same results were | 4 |
clone was fully stable | 4 |
and akt proteins in | 4 |
compared to the non | 4 |
or infected with prrsv | 4 |
the gds strain was | 4 |
than of the cells | 4 |
the annotations in the | 4 |
infection through direct interaction | 4 |
carried out using a | 4 |
a monolayer of vero | 4 |
kda protein similar to | 4 |
effects on the infection | 4 |
did not differ significantly | 4 |
the absence of serum | 4 |
suggested that the compound | 4 |
levels of intracellular viral | 4 |
cell monolayers were washed | 4 |
leader sequence derived from | 4 |
ypk possessed in vitro | 4 |
levels of viral rna | 4 |
antiviral effect of ribavirin | 4 |
diarrhea virus strains in | 4 |
is a determinant of | 4 |
that the s protein | 4 |
novel coronavirus from a | 4 |
and the monolayers were | 4 |
and the supernatant was | 4 |
antibody to the sars | 4 |
of asterisks indicate significant | 4 |
were expressed as the | 4 |
drug was added at | 4 |
of an african green | 4 |
activity of blfp crude | 4 |
the levels of phosphorylated | 4 |
extract were determined by | 4 |
studies on sv in | 4 |
the replication of prrsv | 4 |
and necrosis have been | 4 |
the supernatants were harvested | 4 |
that myricetin possessed anti | 4 |
in the supernatants of | 4 |
with the vero cell | 4 |
of the nucleocapsid protein | 4 |
l to infect ace | 4 |
of action of chloroquine | 4 |
cell infection by tgev | 4 |
near the catalytic centers | 4 |
in the design of | 4 |
inhibitors of cathepsin l | 4 |
were then infected with | 4 |
evaluated by western blot | 4 |
gene sequences of the | 4 |
a limited number of | 4 |
were confirmed by western | 4 |
recovery of infectious virus | 4 |
a dosage of mg | 4 |
with pbs and then | 4 |
ml per well of | 4 |
performed according to the | 4 |
produced in vero cells | 4 |
infectious virus could be | 4 |
the bac plasmid pbelobac | 4 |
a porcine epidemic diarrhea | 4 |
enzyme is a functional | 4 |
during serial passages in | 4 |
of pedv in vero | 4 |
and the infected cells | 4 |
for mouse hepatitis virus | 4 |
h prior to incubation | 4 |
two and four after | 4 |
potential emergence of resistant | 4 |
direct interaction with virus | 4 |
data are compared using | 4 |
hepatitis c virus rna | 4 |
infectious virus was recovered | 4 |
icpc a and coinfection | 4 |
of type i interferons | 4 |
rpoa gene sequence analysis | 4 |
the a protein of | 4 |
intracellular viral rna levels | 4 |
were washed twice in | 4 |
epidemic diarrhea virus into | 4 |
this graph is indicated | 4 |
infectious bronchitis virus and | 4 |
significant when data are | 4 |
into each well and | 4 |
blfp crude extract was | 4 |
of sars coronavirus infection | 4 |
growth kinetics of sars | 4 |
positive for both viruses | 4 |
apoptosis was observed in | 4 |
cell lines that are | 4 |