quadgram

This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

quadgram	frequency
porcine epidemic diarrhea virus	253
of transmissible gastroenteritis virus	157
in the presence of	102
porcine reproductive and respiratory	97
reproductive and respiratory syndrome	96
of transmissible gastroenteritis coronavirus	95
of porcine epidemic diarrhea	89
porcine transmissible gastroenteritis virus	87
and respiratory syndrome virus	83
of the s protein	70
severe acute respiratory syndrome	67
cells were transfected with	58
for the detection of	58
of porcine transmissible gastroenteritis	55
transmissible gastroenteritis virus and	54
as shown in fig	53
in the absence of	52
an important role in	51
cells were infected with	51
feline infectious peritonitis virus	51
of mouse hepatitis virus	48
the results showed that	48
were infected with tgev	47
in the present study	46
and porcine respiratory coronavirus	45
washed three times with	43
on the surface of	41
to transmissible gastroenteritis virus	40
the transmissible gastroenteritis virus	40
acute respiratory syndrome coronavirus	38
gastroenteritis virus and porcine	37
in the united states	37
the s protein of	37
cells were incubated with	36
cells were treated with	36
was used as a	36
used in this study	34
for min at room	34
min at room temperature	34
a member of the	34
were washed three times	33
s and g m	33
porcine epidemic diarrhoea virus	33
has been shown to	32
coronavirus transmissible gastroenteritis virus	32
transmissible gastroenteritis virus in	32
play an important role	32
of porcine respiratory coronavirus	32
epidemic diarrhea virus in	32
cells infected with tgev	31
transmissible gastroenteritis virus infection	31
of the s gene	31
detection of transmissible gastroenteritis	31
infected with tgev at	29
the presence of the	29
hek t cells were	28
it has been shown	28
the end of the	28
in the small intestine	28
as a receptor for	28
play a role in	28
related to transmissible gastroenteritis	27
in the case of	27
with transmissible gastroenteritis virus	27
aminopeptidase n is a	27
and transmissible gastroenteritis virus	27
epidemic diarrhea virus and	26
in the regulation of	26
the coronavirus transmissible gastroenteritis	26
with fetal bovine serum	26
total rna was extracted	25
epidermal growth factor receptor	25
results showed that the	25
of porcine reproductive and	25
at room temperature for	25
loss of enteric tropism	24
studies have shown that	24
work was supported by	24
cells were fixed with	24
transmissible gastroenteritis of swine	24
the molecular biology of	24
avian infectious bronchitis virus	24
on the basis of	24
the tgev s protein	24
from transmissible gastroenteritis virus	24
coronavirus infectious bronchitis virus	24
of feline infectious peritonitis	24
these results suggest that	24
has been shown that	23
for min at rt	23
coronavirus mouse hepatitis virus	23
of antibody binding to	23
the loss of enteric	23
the expression levels of	23
was significantly higher than	23
molecular biology of coronaviruses	23
three times with pbs	23
as well as the	23
this work was supported	23
in the s protein	22
of severe acute respiratory	22
in the s gene	22
sialic acid binding activity	22
and porcine epidemic diarrhea	22
incubated for h at	22
porcine transmissible gastroenteritis coronavirus	22
in transmissible gastroenteritis virus	22
under the control of	21
plays an important role	21
sequence analysis of the	21
these results indicate that	21
gene of transmissible gastroenteritis	21
is a member of	20
antibody binding to the	20
complete genome sequence of	20
we found that tgev	20
of tgev and prcv	20
transmissible gastroenteritis in pigs	20
of the leader rna	20
at an moi of	20
a multiplicity of infection	20
of the coronavirus transmissible	20
of the m protein	20
and g m phases	20
virus and porcine respiratory	20
in vitro and in	20
at a multiplicity of	20
important role in the	19
at the same time	19
the cells were washed	19
of three independent experiments	19
of tgev and pedv	19
supplemented with fetal bovine	19
on the cell surface	19
cell cycle arrest and	19
protein of transmissible gastroenteritis	19
the control of the	19
coronavirus related to transmissible	19
between tgev and prcv	19
transmissible gastroenteritis virus by	19
and then incubated with	19
there was no significant	18
have been shown to	18
found that tgev infection	18
we found that the	18
in the tgev group	18
the degree of antibody	18
antigenic sites of the	18
virus and porcine epidemic	18
degree of antibody binding	18
the sequence of the	18
related porcine respiratory coronavirus	18
nucleotide sequence of the	18
at different time points	18
end of the genome	18
gastroenteritis virus infection induces	17
that tgev infection induced	17
fixed with paraformaldehyde for	17
was no significant difference	17
the porcine transmissible gastroenteritis	17
were fixed with paraformaldehyde	17
n protein of tgev	17
of the tgev s	17
was found to be	17
than that in the	17
vitro and in vivo	17
of the genomic rna	17
rna was extracted from	17
are shown in table	17
ingezim corona diferencial elisa	17
a and orf b	17
of a porcine respiratory	17
cycle arrest and apoptosis	17
for the expression of	17
similar to those of	17
of the cell cycle	16
the sialic acid binding	16
at the end of	16
the tgev m protein	16
expressed in transgenic plants	16
absence of e protein	16
mouse hepatitis virus strain	16
against transmissible gastroenteritis virus	16
cells were washed with	16
significantly higher than that	16
a major receptor for	16
four major antigenic sites	16
with paraformaldehyde for min	16
of the transmissible gastroenteritis	16
cells were pretreated with	16
of the small intestine	16
orf a and orf	16
been shown to be	16
the s protein is	16
were washed twice with	16
the structure of the	16
detection of porcine epidemic	16
structure of transmissible gastroenteritis	16
in this study were	16
ileum and colon epithelial	16
higher than that of	16
from three independent experiments	16
of p and jnk	15
are shown in fig	15
induced by tgev infection	15
the authors declare that	15
in the loss of	15
transcription of tgev gene	15
pathogenesis of transmissible gastroenteritis	15
the detection limit of	15
of the spike protein	15
in addition to the	15
has been reported to	15
hepatitis virus strain a	15
is a receptor for	15
the presence of a	15
was added to each	15
it is possible that	15
of the porcine transmissible	15
in cells infected with	15
and incubated for h	15
n is a major	15
porcine intestinal epithelial cells	15
that tgev n protein	15
is a major receptor	15
cells were cotransfected with	15
with the exception of	15
the development of a	15
of porcine epidemic diarrhoea	15
cells treated with lp	15
and colon epithelial cells	15
major receptor for the	15
dna and rna viruses	14
j cells treated with	14
with tgev for h	14
purdue strain of tgev	14
showed that tgev infection	14
the expression of the	14
than that of the	14
as shown in figure	14
cells were grown in	14
added to each well	14
h at room temperature	14
in a co incubator	14
a transmissible gastroenteritis in	14
swine transmissible gastroenteritis virus	14
on the other hand	14
cells were washed three	14
with porcine respiratory coronavirus	14
was added to the	14
the s gene of	14
for mouse hepatitis virus	14
of the virus in	14
the porcine epidemic diarrhea	14
were detected in the	14
a functional receptor for	14
activation of p signaling	14
middle east respiratory syndrome	14
higher than that in	14
our results showed that	14
porcine epidemic diarrhea in	14
the spike gene of	14
the epidermal growth factor	14
infected cells per well	14
infected with tgev for	14
the sensitivity of the	14
for h at room	14
the leader rna sequence	14
for porcine epidemic diarrhea	14
the level of p	14
were washed with pbs	14
have shown that the	14
the detection of tgev	13
was observed in the	13
of apn positive cells	13
play important roles in	13
these results suggested that	13
declare that they have	13
that they have no	13
of the n gene	13
is a functional receptor	13
and systemic immune responses	13
to that of the	13
the ingezim corona diferencial	13
cells were cultured in	13
the tips of the	13
to those of the	13
for the development of	13
were found to be	13
is involved in the	13
tgev and prcv strains	13
authors declare that they	13
be involved in the	13
binding of tgev to	13
the nature of the	13
was supported by the	13
is not required for	13
amino acid changes at	13
with tgev at moi	13
were performed as described	13
induces cell cycle arrest	13
passages in tissue culture	13
acid changes at the	13
induced cell cycle arrest	13
swinecheck tgev prcv recombinant	13
virus and transmissible gastroenteritis	13
similar to that of	13
cells infected with rtgev	13
the coronavirus infectious bronchitis	13
coe and rlppg f	13
incubated for min at	13
enteric tropism and virulence	13
of the porcine epidemic	13
the course of the	13
the spike protein of	13
activation of p mapk	12
cv vero adapted strain	12
that in the tgev	12
tgev at an moi	12
monoclonal antibodies to the	12
the absence of e	12
transmissible gastroenteritis virus of	12
pigs inoculated with tgev	12
washed with pbs and	12
the detection of porcine	12
at the time of	12
the m protein is	12
cells with bound tgev	12
was lower than that	12
is a determinant of	12
the leader rna and	12
coronaviruses as the gene	12
amino acid sequences of	12
cd cd t cells	12
as the gene source	12
pellet was resuspended in	12
the surface of the	12
was performed as described	12
in agreement with the	12
were incubated with tgev	12
against transmissible gastroenteritis coronavirus	12
the svanovir tgev prcv	12
the villous enterocytes of	12
the gene source of	12
transmissible gastroenteritis coronavirus is	12
of tgev n protein	12
is responsible for the	12
the m protein of	12
in contrast to the	12
s protein of tgev	12
were incubated for h	12
as a negative control	12
critical epitopes in transmissible	12
performed as described previously	12
epitopes in transmissible gastroenteritis	12
of the viral genome	12
transmissible gastroenteritis virus spike	12
was used to detect	12
room temperature for h	12
lower than that of	12
isolation of a porcine	12
the ability of the	12
with tgev at an	12
spike gene of transmissible	12
epidemic diarrhea virus strain	12
in any of the	12
may be involved in	12
cell cycle arrest at	12
is a highly contagious	12
it is difficult to	12
were cloned into the	12
associated with diarrhea in	12
in the cytoplasm of	12
are located within the	12
had no effect on	12
the presence of tgev	12
at a dilution of	12
gastroenteritis coronavirus is a	11
were added to the	11
assay for the detection	11
the percentage of apn	11
science foundation of china	11
that the spike gene	11
after h of incubation	11
strain of transmissible gastroenteritis	11
it is likely that	11
determinant of its enteric	11
and cloned into the	11
coronavirus is a determinant	11
the porcine reproductive and	11
the tgev nucleoprotein gene	11
early in tgev infection	11
nuclei were stained with	11
its enteric tropism and	11
demonstrates that the spike	11
and evolution of the	11
by transmissible gastroenteritis virus	11
detection limit of the	11
cells infected with the	11
epithelial cells of the	11
the replication of tgev	11
mesenteric lymph node cells	11
in accordance with the	11
these data indicate that	11
sites of the coronavirus	11
antigenic sites a and	11
are located on the	11
no conflict of interest	11
mutations in the s	11
epidemic diarrhea virus infection	11
result in the loss	11
porcine hemagglutinating encephalomyelitis virus	11
of the virus to	11
transmissible gastroenteritis virus on	11
end of the leader	11
enteric coronavirus related to	11
changes at the n	11
these results indicated that	11
two amino acid changes	11
a determinant of its	11
the results of the	11
national natural science foundation	11
of the murine coronavirus	11
in the formation of	11
transmissible gastroenteritis virus neutralization	11
by transmissible gastroenteritis coronavirus	11
at g for min	11
in the induction of	11
the enteric tropism of	11
was similar to that	11
tgev prcv recombinant elisa	11
mice orally immunized with	11
a porcine respiratory coronavirus	11
of its enteric tropism	11
for rapid detection of	11
a cellular receptor for	11
tips of the villi	11
of tgev infection on	11
with tgev at a	11
major antigenic sites of	11
group than in the	11
and characterization of a	11
t cells were transfected	11
cell cycle arrest in	11
east respiratory syndrome coronavirus	11
antibodies for h at	11
the role of apn	11
of the e glycoprotein	11
at s and g	11
group was significantly higher	11
cells were washed twice	11
natural science foundation of	11
functional receptor for the	11
used as a control	11
was expressed as the	10
virus are located on	10
protein result in the	10
for h prior to	10
the small intestine and	10
recombination demonstrates that the	10
interpretation of suspect results	10
the cells were fixed	10
of apn negative cells	10
in a co atmosphere	10
the transmissible gastroenteritis coronavirus	10
may play a role	10
cells which were fixed	10
in the lamina propria	10
isolation and identification of	10
to the cell surface	10
j cells infected with	10
in porcine intestinal epithelial	10
the cells released from	10
in the spike protein	10
is not essential for	10
tgev n protein was	10
the small intestine of	10
economic losses in the	10
paraformaldehyde for min and	10
at the cell surface	10
in the di rna	10
pedv and tgev infection	10
are listed in table	10
located on the amino	10
in the pbs group	10
it is important to	10
the copy number of	10
by the addition of	10
the beginning of the	10
were stained with hoechst	10
the cell culture adapted	10
diarrhea virus in the	10
the s and g	10
the binding of the	10
was inserted into the	10
targeted recombination demonstrates that	10
was carried out using	10
were isolated from the	10
analyzed by western blotting	10
in the cells released	10
and porcine epidemic diarrhoea	10
the swinecheck tgev prcv	10
gastroenteritis coronavirus spike protein	10
the piglets in the	10
is consistent with the	10
international committee on taxonomy	10
was used as the	10
villous enterocytes of newborn	10
gastroenteritis virus spike protein	10
transmissible gastroenteritis virus from	10
the tropism of the	10
m and e proteins	10
tgev was detected by	10
de groot et al	10
in the control of	10
internal entry of ribosomes	10
for pedv and tgev	10
were analyzed by western	10
largest rna virus genome	10
transmissible gastroenteritis coronavirus genome	10
mucosal and systemic immune	10
the viral genomic rna	10
inoculated with tgev strain	10
gastroenteritis virus on the	10
in the serum of	10
pigs of all ages	10
followed by goat anti	10
for the first time	10
spike protein result in	10
in a variety of	10
results suggest that tgev	10
hepatitis b surface antigen	10
the coronavirus mouse hepatitis	10
coronavirus spike protein result	10
was detected in the	10
from porcine transmissible gastroenteritis	10
antigenic structure of transmissible	10
performed according to the	10
the largest rna virus	10
in this study was	10
a role in the	10
were observed in the	10
was significantly lower than	10
transmissible gastroenteritis coronavirus spike	10
under a fluorescence microscope	10
and st cells were	10
probably due to the	10
evolution of the purdue	9
recombinant lactic acid bacteria	9
intestinal epithelial cell line	9
results were interpreted as	9
to porcine reproductive and	9
studies on transmissible gastroenteritis	9
de haan et al	9
of swine transmissible gastroenteritis	9
suspect results were interpreted	9
losses in the swine	9
hoechst for min at	9
genome sequence of transmissible	9
transmissible gastroenteritis virus are	9
cells were inoculated with	9
to infection with tgev	9
could be detected in	9
plays a role in	9
our results indicate that	9
were found in the	9
in the culture medium	9
terminal half of spike	9
from porcine respiratory coronavirus	9
sequence of transmissible gastroenteritis	9
from mpi to mpi	9
the deduced amino acid	9
in comparison with the	9
the causative agent of	9
it has been reported	9
transmissible gastroenteritis virus entry	9
from the tips of	9
of a against tgev	9
these results show that	9
room temperature for min	9
the presence or absence	9
have no competing interests	9
epidemic diarrhea virus strains	9
immunity against transmissible gastroenteritis	9
of lactic acid bacteria	9
by the use of	9
were randomly divided into	9
the cells were then	9
diagnosis of transmissible gastroenteritis	9
arrest at s and	9
the amino acid sequence	9
the size of the	9
on taxonomy of viruses	9
it is worth noting	9
the authors declare no	9
receptor for mouse hepatitis	9
on the day of	9
with the tgev m	9
demonstrated that tgev infection	9
was measured at nm	9
infected cells which were	9
gastroenteritis virus are located	9
orally immunized with rlppg	9
of avian infectious bronchitis	9
presence or absence of	9
way analysis of variance	9
is worth noting that	9
of the purdue virus	9
they have no competing	9
t cells were cotransfected	9
cd ssc low cells	9
sites c and b	9
the difference in the	9
times with pbs and	9
the case of the	9
sites a and d	9
the pellet was resuspended	9
it has been demonstrated	9
transmissible gastroenteritis coronavirus pur	9
of the pfcrn gene	9
with diarrhea in swine	9
the cells were incubated	9
and phylogenetic analysis of	9
that the levels of	9
at h post transfection	9
in cells transfected with	9
results suggest that the	9
compared with that of	9
the early stage of	9
the nucleotide sequence of	9
the international committee on	9
cd ssc high cells	9
residues involved in the	9
stained with hoechst for	9
copy number of the	9
of the e protein	9
the activation of p	9
and infected with tgev	9
was found in the	9
emergence of porcine epidemic	9
t g e v	9
and c antigen sites	9
clone and evolution of	9
classical swine fever virus	9
by sodium dodecyl sulfate	9
were used in this	9
of the tgev spike	9
has been demonstrated that	9
the s protein was	9
bca protein assay reagent	9
the porcine respiratory coronavirus	9
the specificity of the	9
when suspect results were	9
de diego et al	9
immunized with tgev antigen	9
of the tgev genome	9
virulent and attenuated strains	9
the effects of tgev	9
the mucosal immune response	9
conventional duplex pcr rt	9
to the s protein	9
the purdue virus cluster	9
an increase in the	9
committee on taxonomy of	9
are involved in the	9
piglets in the tgev	9
the animals immunized with	9
and g m phase	9
region of the s	9
transmissible gastroenteritis and porcine	9
mad clone and evolution	9
of the assay was	9
which were fixed with	9
were incubated with the	9
in the golgi complex	9
the results indicated that	9
the formation of the	9
a receptor for pdcov	9
involved in the regulation	9
for the presence of	9
the number of cd	9
the amount of viral	9
member of the coronaviridae	9
incubated with tgev at	9
these findings suggest that	9
as shown in table	9
with hoechst for min	9
the cell membrane and	9
cells were stained with	9
i i f test	9
protective lactogenic immunity against	9
b and c antigen	9
virus in the united	9
the feline infectious peritonitis	9
at a concentration of	9
in the respiratory tract	9
pedv cv vero adapted	9
both ileum and colon	9
determinants are located within	9
was used to confirm	9
half of spike glycoprotein	8
avian coronavirus infectious bronchitis	8
on transmissible gastroenteritis of	8
three major structural proteins	8
these data indicated that	8
of the family coronaviridae	8
the rapid detection of	8
within the same domain	8
the viral spike protein	8
in the feces of	8
structural components from porcine	8
ra plus tgev s	8
terminal domain of the	8
by agarose gel electrophoresis	8
antigenic homology among coronaviruses	8
binding to the m	8
common mucosal immune system	8
promotes transmissible gastroenteritis virus	8
our understanding of the	8
the interpretation of suspect	8
membrane of infected cells	8
to determine whether the	8
in the viral envelope	8
analysis of the porcine	8
was calculated using the	8
in the presence or	8
the invasion of tgev	8
as a result of	8
for the formation of	8
with different concentrations of	8
der most et al	8
and the cells were	8
cellular receptor for pedv	8
with mouse monoclonal anti	8
are summarized in table	8
the purdue strain of	8
in relation to the	8
in an attempt to	8
serves as a receptor	8
reactivity between tgev and	8
with a mixture of	8
amino acid sequence of	8
t cells were co	8
has been reported that	8
in complex with the	8
in a dose dependent	8
the enteropathogenic coronavirus tgev	8
a dose dependent manner	8
located within the same	8
to jurisdictional claims in	8
the molecular basis of	8
antigenic relationship of the	8
strains of transmissible gastroenteritis	8
and feline infectious peritonitis	8
of the s glycoprotein	8
nature remains neutral with	8
the tgev n protein	8
can be used for	8
molecular features and virus	8
did not neutralize the	8
recombinant swinepox virus expressing	8
and the expression of	8
in the swine industry	8
washed twice with pbs	8
sites a and b	8
regard to jurisdictional claims	8
the leader rna sequences	8
of structural components from	8
aminopeptidase n and its	8
the m gene of	8
and its inhibition by	8
virus in cell culture	8
a novel swine enteric	8
at moi for h	8
the enteric and respiratory	8
the culture medium was	8
of murine hepatitis virus	8
antibody was purchased from	8
with bound tgev purdue	8
was performed using the	8
epidemic diarrhoea virus and	8
cells were fixed in	8
of the spike glycoprotein	8
maps and institutional affiliations	8
other cov n proteins	8
porcine respiratory coronavirus in	8
were significantly higher than	8
the pathogenesis of transmissible	8
oral corn vaccine on	8
of coronavirus mouse hepatitis	8
in ml of pbs	8
mediated by the viral	8
in dmem supplemented with	8
structure and genome expression	8
receptor for the enteropathogenic	8
the n terminus of	8
as a source of	8
have been reported to	8
rna was isolated from	8
rapid detection of transmissible	8
for the enteropathogenic coronavirus	8
as a selective marker	8
jurisdictional claims in published	8
the basis of the	8
expression of tgev n	8
cells were purchased from	8
for the rapid detection	8
than in the tgev	8
domain of the transmissible	8
inhibition by neutralizing antibodies	8
containing fetal calf serum	8
clarified by centrifugation at	8
of alpha interferon by	8
cells in the presence	8
binding and neutralization determinants	8
of the feline infectious	8
the same domain of	8
version of this article	8
infected cells compared to	8
min at rt and	8
neutralization determinants are located	8
neutral with regard to	8
remains neutral with regard	8
internalized into the cytoplasm	8
tgev at moi for	8
to the cell membrane	8
were approved by the	8
and mucosal immune responses	8
tgev at a multiplicity	8
the i i f	8
on the plasma membrane	8
virus genome as an	8
antigenicity of structural components	8
in this study we	8
attenuated and virulent tgev	8
with bound tgev miller	8
of the structural proteins	8
and neutralization determinants are	8
of the papn ectodomain	8
as the secondary antibody	8
its inhibition by neutralizing	8
is one of the	8
corn vaccine on days	8
particle associated with diarrhea	8
the inhibitory activity of	8
common to five isolates	8
with regard to jurisdictional	8
terminus of transmissible gastroenteritis	8
of the nucleoprotein gene	8
for the study of	8
virus common to five	8
springer nature remains neutral	8
in phosphate buffered saline	8
glycoprotein of transmissible gastroenteritis	8
j cells were pretreated	8
this is the first	8
that the leader rna	8
the expression of small	8
may be required for	8
goat serum for h	8
porcine aminopeptidase n is	8
cycle arrest at s	8
point mutations in the	8
of spike glycoprotein s	8
selected characteristics of a	8
high morbidity and mortality	8
of a murine coronavirus	8
grown in the presence	8
was higher than that	8
claims in published maps	8
the interaction between the	8
the national natural science	8
induction of alpha interferon	8
is required for efficient	8
published maps and institutional	8
for min and the	8
same domain of the	8
at the indicated time	8
for tgev and pcv	8
the inhibitory effect of	8
with respect to the	8
the antigenic sites of	8
activity of a against	8
we are grateful to	8
in published maps and	8
for pcv and tgev	8
was not affected by	8
investigated the effects of	8
gastroenteritis virus by rt	8
in the control group	8
inhibitory activity of a	8
previous study showed that	8
were inoculated with tgev	8
mrna expression levels of	8
serum for h at	8
between and copies ml	8
van der most et	8
samples were collected from	8
and sequence analysis of	7
in the enteric tract	7
in the cell membrane	7
significantly higher in the	7
pi k akt pathway	7
entry mediated by the	7
with fetal calf serum	7
that tgev infection could	7
for the design of	7
third of the genome	7
were shown to be	7
the membrane was blocked	7
acid changes in the	7
in st cells and	7
cells were used as	7
the s proteins of	7
enteric tropism of tgev	7
the interaction between tgev	7
characteristics of a cytopathogenic	7
with bovine serum albumin	7
it is not clear	7
a major cause of	7
were between and copies	7
us porcine epidemic diarrhea	7
the result showed that	7
were compared with those	7
a epitope of the	7
determine the effect of	7
st cells infected with	7
online version of this	7
has also been shown	7
and viral genomic sequences	7
no significant difference in	7
binding activity with the	7
a g magnetic beads	7
porcine respiratory coronavirus differs	7
through activation of p	7
cd t cells in	7
vivo virus replication and	7
diarrhea in suckling piglets	7
of pedv and tgev	7
strain was isolated from	7
of the present study	7
igg fitc labeled antibodies	7
g for min at	7
was performed with the	7
apoptosis via activation of	7
all experiments were performed	7
and differentiation from porcine	7
tgev prcv blocking elisas	7
is an enveloped virus	7
and sequence of the	7
in the correct orientation	7
gastroenteritis virus entry into	7
differs from transmissible gastroenteritis	7
the severe acute respiratory	7
s protein connect the	7
the tgev spike protein	7
compared with those of	7
and identification of porcine	7
end of the genomic	7
western blot analysis showed	7
days after infection and	7
be used as a	7
from the viral membrane	7
bases of coronavirus attachment	7
based and duplex mmps	7
the presence of maternal	7
entry into intestinal epithelial	7
gastroenteritis virus by a	7
sodium dodecyl sulfate polyacrylamide	7
of a cytopathogenic virus	7
data indicate that the	7
african green monkey kidney	7
in intestinal epithelial cells	7
from the jejunum of	7
the mrna start sites	7
and the supernatant was	7
incubated with mouse monoclonal	7
with tgev antigen alone	7
a cytopathogenic virus common	7
for the loss of	7
of transmissible gastroenteritis in	7
a previous study showed	7
human immunodeficiency virus type	7
it is interesting to	7
results showed that tgev	7
in pbs for min	7
genes a and b	7
in the early stage	7
propagated in st cells	7
and the sensitivity of	7
diarrhea virus in china	7
gene is not essential	7
and van der zeijst	7
immune responses induced by	7
mechanisms of coronavirus cell	7
infection or vaccination with	7
responsible for the loss	7
targeting the m gene	7
tissue culture infective dose	7
acid binding activity of	7
between the leader rna	7
at days of age	7
the titers of tgev	7
structural bases of coronavirus	7
at a density of	7
of coronavirus cell entry	7
gastroenteritis virus infection in	7
at room temperature and	7
a wide range of	7
virus by a few	7
of the coronavirus mouse	7
binding domain complexed with	7
via activation of p	7
protein a g magnetic	7
coronavirus attachment to host	7
virus and porcine deltacoronavirus	7
were used to infect	7
acid binding activity with	7
that there is a	7
enteropathogenicity of transmissible gastroenteritis	7
and washed three times	7
was derived from the	7
to the family coronaviridae	7
fixed cells which were	7
higher in the lp	7
differentiation from porcine respiratory	7
a log reduction factor	7
challenge with a virulent	7
the data indicate that	7
and expression of the	7
the indirect fluorescent antibody	7
components from porcine transmissible	7
cells released from g	7
the absence of rna	7
to determine the effect	7
a key role in	7
replication of tgev in	7
for the production of	7
and tropism of transmissible	7
the virus in the	7
the total number of	7
the s protein connect	7
n and its inhibition	7
transmissible gastroenteritis coronavirus gene	7
at the beginning of	7
activity with the enteropathogenicity	7
signal transducer and activator	7
was not significantly different	7
into hek t cells	7
and hemagglutinating encephalomyelitis virus	7
the cells were infected	7
and cultured for h	7
virus infection induces cell	7
porcine intestinal columnar epithelial	7
that tgev infection induces	7
three times with pbst	7
of the tgev n	7
with the enteropathogenicity of	7
pcr and differentiation from	7
in the pathogenesis of	7
m and s determinants	7
evolution and tropism of	7
data are presented as	7
as well as in	7
compared to that in	7
the enteropathogenicity of transmissible	7
and immunogenicity of recombinant	7
oral lactobacillus casei vaccine	7
have been detected in	7
divided into three groups	7
the common mucosal immune	7
have been demonstrated to	7
the binding of tgev	7
of sows with a	7
the difference between the	7
followed by incubation with	7
classification and nomenclature of	7
the tgev and prcv	7
for transmissible gastroenteritis virus	7
the viral life cycle	7
of the human coronavirus	7
terminal half of the	7
terminal region of the	7
attachment to host aminopeptidase	7
at the indicated times	7
after infection or vaccination	7
the e glycoprotein of	7
might be involved in	7
of cd cd t	7
by the viral spike	7
respiratory coronavirus differs from	7
binding to the n	7
the detection of the	7
of the tgev and	7
coronavirus differs from transmissible	7
of coronavirus attachment to	7
cells compared with the	7
the innate immune response	7
as a cellular receptor	7
relationship of the feline	7
percentage of apn positive	7
transducer and activator of	7
role of apn in	7
in the supernatants of	7
of tgev has been	7
apn and egfr synergistically	7
diarrhea virus and porcine	7
infected or infected with	7
followed by cycles of	7
dodecyl sulfate polyacrylamide gel	7
method of estimating fifty	7
virus replication and virulence	7
when the cells were	7
for the synthesis of	7
cytopathogenic virus common to	7
that the amount of	7
well tissue culture plates	7
transcription recombinase polymerase amplification	7
of tgev in the	7
supplemented with fetal calf	7
and nomenclature of viruses	7
a receptor for tgev	7
in vivo and in	7
which was consistent with	7
in vivo virus replication	7
a simple method of	7
one of the most	7
genetic evolution and tropism	7
immunized with rlppg f	7
compared with the control	7
in the production of	7
the results suggest that	7
did not affect the	7
tgev miller and purdue	7
a large number of	7
pi k akt and	7
of the coronaviridae family	7
both tgev and prcv	7
the s protein and	7
the mouse hepatitis virus	7
were carried out in	7
of intestinal epithelial cells	7
infectious bursal disease virus	7
enterocytes of newborn pigs	7
is distinct from the	7
for the pathogenesis of	7
gastroenteritis virus in swine	7
after an incubation period	7
were used as a	7
of the virus of	7
infected tissue culture fluid	7
could be due to	7
into intestinal epithelial cells	7
can be used to	7
presence of maternal antibodies	7
for h and then	7
and the absorbance was	7
protein connect the sialic	7
not essential for viral	7
that the m protein	7
in the viral genome	7
to host aminopeptidase n	7
coronavirus cell entry mediated	7
membrane was blocked with	7
is required for the	7
the online version of	7
american type culture collection	7
the bottom of the	7
amino acid changes in	7
were considered statistically significant	7
cell entry mediated by	7
for simultaneous detection of	7
of differentially expressed lncrnas	7
the separated proteins were	7
was based on the	7
infection with the porcine	7
swine after infection or	7
an incubation period of	7
tgev is an enveloped	7
the extracellular region of	7
entry into host cells	7
as a function of	7
in the tgev genome	7
compared to that of	7
of the af mab	7
the mrna levels of	7
was removed and the	7
p and jnk phosphorylation	7
simple method of estimating	7
host aminopeptidase n and	7
porcine epidemic diarrhea coronavirus	7
with the results of	7
is closely related to	7
animals immunized with tgev	7
under the same conditions	7
the absorbance was measured	7
gastroenteritis virus of swine	7
against porcine epidemic diarrhea	7
the intestinal epithelial cells	7
the s protein in	7
the pedv s protein	7
in significant economic losses	7
may be related to	7
cells were seeded in	7
were transfected with the	7
connect the sialic acid	7
with porcine epidemic diarrhoea	7
extracellular region of tfr	7
that is responsible for	7
with the porcine respiratory	7
the epithelial cells of	7
the region of the	7
the tgev s gene	7
were transferred to a	6
results indicated that the	6
in pigs inoculated with	6
mammalian and avian coronaviruses	6
coronavirus gene is not	6
that the s protein	6
protein is essential for	6
results demonstrated that the	6
and fecal shedding of	6
the infectious bronchitis virus	6
the mechanism of rna	6
rna and dna genomes	6
tissue culture infectious dose	6
value obtained with tgev	6
primary ileum epithelial cells	6
tgev n protein expressed	6
the n gene of	6
st cells were infected	6
for systematic assembly of	6
significantly increased at h	6
of gammacoronavirus and deltacoronavirus	6
in tgev n protein	6
of kda expressed by	6
with tcid animal of	6
betacoronavirus and avian coronaviruses	6
ileum epithelial cells were	6
absorbance was measured at	6
and avian coronaviruses in	6
in the genus deltacoronavirus	6
receptor for the entero	6
the activation of the	6
then washed three times	6
be used for the	6
gastroenteritis coronavirus gene is	6
norwalk virus capsid protein	6
been shown that the	6
coronavirus defective interfering rna	6
of primary porcine enterocytes	6
the a epitope of	6
roswell park memorial institute	6
cells were synchronized at	6
by the oral route	6
coe of kda expressed	6
analyzed by flow cytometry	6
cells at h p	6
the loss of the	6
of neutralizing antibodies in	6
vaccination with transmissible gastroenteritis	6
activity and promotes transmissible	6
in the development of	6
these data suggest that	6
of spleen lymphocytes from	6
milk of swine after	6
were then incubated with	6
the cellular receptor for	6
like particle associated with	6
and attenuated strains of	6
suggest that tgev infection	6
swine transmissible gastroenteritis coronavirus	6
enjuanes and van der	6
and avian coronaviruses as	6
not essential but influences	6
but not in the	6
strand cdna synthesis kit	6
tgev infection and replication	6
it was found that	6
a final concentration of	6
of bovine coronavirus is	6
as a positive control	6
better understanding of the	6
homology among coronaviruses related	6
rapid and efficient detection	6
cholesterol depletion from the	6
positive cells with bound	6
the phosphorylation of cofilin	6
protein of tgev and	6
the reciprocal of the	6
the s protein has	6
studies have found that	6
diarrhea virus spike protein	6
apn is a receptor	6
the economic impact of	6
serial dilutions of the	6
of tgev n gene	6
the expression of cd	6
of tgev spike protein	6
an infectious bacterial artificial	6
the d antigenic site	6
produced in transgenic plants	6
cells were maintained in	6
born to vaccinated sows	6
was carried out as	6
was shown to be	6
of the economic impact	6
and milk of swine	6
distinct from the enzymatic	6
the leader and ig	6
the cells were lysed	6
discussed in section v	6
and replication of tgev	6
of swine after infection	6
the detection limits of	6
our results suggest that	6
in a swine herd	6
wild type and mutant	6
supports bat coronaviruses as	6
antigens on the surface	6
genome as an infectious	6
has not been determined	6
source of alphacoronavirus and	6
infection induces apoptosis through	6
strains of porcine epidemic	6
interacting with n protein	6
porcine respiratory coronavirus from	6
single amino acid changes	6
in colostrum and milk	6
was amplified by pcr	6
it was shown that	6
susceptible cells did not	6
by the finding that	6
an equal volume of	6
was significantly higher in	6
localization of apn and	6
and differential diagnosis of	6
have been used to	6
to be involved in	6
of the same age	6
compatible ends into the	6
bel rv g p	6
translocation to the nucleus	6
times with lysis buffer	6
leader complex binding site	6
transmissible gastroenteritis coronavirus neutralization	6
we investigated the effects	6
pcr assay for tgev	6
similar to transmissible gastroenteritis	6
from the enzymatic site	6
virus infection induces apoptosis	6
a virulent strain of	6
encodes four structural proteins	6
apoptosis through activation of	6
than those in the	6
compared with the mock	6
end and a poly	6
a putative second receptor	6
growth factor receptor regulates	6
role of transmembrane glycoprotein	6
reproduced with permission from	6
density value obtained with	6
by low speed centrifugation	6
proteins of interest by	6
m protein of tgev	6
cells compared to that	6
prepared in our laboratory	6
the effect of tgev	6
the nucleocapsid protein of	6
statistical analysis was performed	6
polypeptide structure of transmissible	6
by western blotting using	6
activation of the ifn	6
deltacoronavirus supports bat coronaviruses	6
the activities of caspase	6
the respiratory tract and	6
respiratory syndrome virus infection	6
academy of agricultural sciences	6
by any of the	6
levels of neutralizing antibodies	6
major structural proteins of	6
as an infectious bacterial	6
antigenic site of the	6
the tgev strain purdue	6
the polypeptide structure of	6
were also observed in	6
the gene encoding the	6
characterization and pathogenesis of	6
j cells were infected	6
cells were collected and	6
that is distinct from	6
as the optical density	6
interesting to note that	6
the effects of mir	6
protein was used as	6
animals infected with the	6
and activator of transcription	6
to the leader rna	6
of the three commercial	6
spleen lymphocytes from mice	6
rna was extracted using	6
resuspended in ml of	6
the virulent and attenuated	6
not included in the	6
genome sequence of a	6
lower than that in	6
avian coronaviruses as the	6
receptor interaction reside within	6
interaction reside within a	6
less than weeks old	6
virus was detected in	6
of the partial s	6
are presented as the	6
with the m protein	6
apn negative cells were	6
used as the secondary	6
is not essential but	6
cells were fixed and	6
analysis was performed using	6
the induction of ifn	6
against swine transmissible gastroenteritis	6
resulting in significant economic	6
that the expression of	6
using purified tgev as	6
time pcr was performed	6
were incubated for min	6
caused by transmissible gastroenteritis	6
essential but influences in	6
assess the role of	6
response specific for pedv	6
t cells in the	6
added to the cells	6
the plates were washed	6
multiplicity of infection of	6
j cells were transfected	6
transcripts of porf a	6
and apoptosis through activation	6
data indicate that rtgev	6
vomiting and wasting disease	6
the finding that the	6
were collected at different	6
of the peplomer protein	6
factor receptor regulates cofilin	6
based on the s	6
in order to determine	6
balb c mice were	6
cofilin activity and promotes	6
the infectivity of tgev	6
studies have demonstrated that	6
of the tgev nucleoprotein	6
of the international committee	6
f i p v	6
n terminus of the	6
the complete genome sequence	6
intestinal columnar epithelial cells	6
characterization of a novel	6
sequence of the peplomer	6
the m and e	6
both pedv and tgev	6
vaccines for porcine epidemic	6
mock infected or infected	6
was obtained from the	6
st cells stably expressing	6
has been previously described	6
are present in the	6
a virulent us pedv	6
protein was expressed in	6
cell lysates were prepared	6
expressed as the optical	6
negative cells with bound	6
kindly provided by dr	6
receptor for the pedv	6
each of the two	6
and pathogenesis of transmissible	6
essential for the transmissible	6
apoptosis induced by tgev	6
against pedv and tgev	6
avian coronaviruses in the	6
phase of the cell	6
of cell cycle arrest	6
generating recombinant plasmid ppg	6
used as a source	6
among coronaviruses related to	6
important role in tgev	6
and promotes transmissible gastroenteritis	6
identification of a new	6
around the cell membrane	6
investigate the effect of	6
of the genome encodes	6
for the transmissible gastroenteritis	6
n that is distinct	6
containing normal goat serum	6
d antigenic site of	6
used to confirm the	6
proteins were transferred to	6
coe at saci and	6
deduced amino acid sequences	6
shown in fig a	6
dmem supplemented with fbs	6
cells were permeabilized with	6
j cells were incubated	6
of enteric tropism in	6
by porcine epidemic diarrhea	6
molecular characterization and pathogenesis	6
the nuclear translocation of	6
samples were between and	6
of the coronavirus infectious	6
effect of cholesterol depletion	6
protein concentrations were measured	6
is possible that the	6
the formation of virus	6
systematic assembly of large	6
vivo and in vitro	6
five isolates from transmissible	6
the m and s	6
influences in vivo virus	6
centrifugation at g for	6
lactogenic immunity against tgev	6
or vaccination with transmissible	6
in the intestinal tract	6
by the presence of	6
the differentially expressed proteins	6
mechanisms of transmissible gastroenteritis	6
for the pedv coronavirus	6
relative to the optical	6
been reported to be	6
large rna and dna	6
of tgev and the	6
group was comprised of	6
tropism of transmissible gastroenteritis	6
sd from three independent	6
in the colostrum and	6
regulates cofilin activity and	6
the cells transfected with	6
in the gut of	6
but influences in vivo	6
within a domain of	6
supported by the national	6
that the n protein	6
porcine intestinal epithelial cell	6
has been detected in	6
of the tgev rbd	6
discovery of seven novel	6
is interesting to note	6
divided into four groups	6
of the bovine coronavirus	6
the mucosal immune system	6
immunity to transmissible gastroenteritis	6
cells derived from the	6
the upper respiratory tract	6
the plasma membrane of	6
the genus deltacoronavirus supports	6
encoding gene of transmissible	6
c in a co	6
of tgev was detected	6
the rest of the	6
immune responses in the	6
monoclonal antibodies directed against	6
predicted to encode a	6
correlated positively with time	6
and betacoronavirus and avian	6
has been identified as	6
seven novel mammalian and	6
fold higher than that	6
assembly of large rna	6
protein induces cell cycle	6
between tgev s and	6
to assess the role	6
to five isolates from	6
herpes simplex virus type	6
entry of tgev was	6
be related to the	6
on the villous enterocytes	6
a receptor for human	6
gene source of gammacoronavirus	6
glycoprotein s from tgev	6
for each of the	6
the site of virus	6
respiratory and fecal shedding	6
for detection of tgev	6
circulating in a swine	6
the effect of these	6
sense rna genome of	6
inhibit the replication of	6
indirect fluorescent antibody technique	6
previous studies have shown	6
of st cells were	6
have been described in	6
an attenuated variant of	6
infectious peritonitis virus to	6
h n influenza virus	6
epidemic diarrhea virus from	6
the surface of infected	6
and the percentage of	6
rna virus genome as	6
is involved in tgev	6
the effect of cholesterol	6
tc bel rv g	6
the optical density value	6
of the method was	6
transmissible gastroenteritis virus a	6
the fixed cells were	6
tissue culture fluid was	6
the levels of anti	6
s protein interacts with	6
a consequence of the	6
coronaviruses in the genus	6
of p mapk pathway	6
commercial tgev prcv blocking	6
the antigenic relationship of	6
leader sequence at the	6
were analyzed by a	6
be responsible for the	6
with the cell culture	6
novel mammalian and avian	6
followed by the addition	6
of porcine enteric coronaviruses	6
entry of porcine epidemic	6
the synthesis of a	6
an integral membrane protein	6
n is a receptor	6
of large rna and	6
determinants essential for the	6
tgev and prcv s	6
the apn expression to	6
by a few genomic	6
virus entry into intestinal	6
infectious bacterial artificial chromosome	6
it has been suggested	6
novel swine enteric alphacoronavirus	6
in viral rna synthesis	6
genetic analysis of porcine	6
a few genomic deletions	6
may play an important	6
effects of tgev infection	6
were inserted into the	6
n is a functional	6
analysis of porcine respiratory	6
was defined as the	6
transmissible gastroenteritis virus model	6
the presence of rna	6
strategy for systematic assembly	6
infected with tgev or	6
stably transformed with the	6
the proportion of cells	6
tgev infection on the	6
in ileum epithelial cells	6
in the jejunum of	6
used as a negative	6
is associated with the	6
end of the tgev	6
as evidenced by the	6
were transfected with nm	6
epidemic diarrhea virus spike	6
and matrix protein genes	6
genus deltacoronavirus supports bat	6
alphacoronavirus and betacoronavirus and	6
human aminopeptidase n is	6
the membranes were blocked	6
attenuated transmissible gastroenteritis virus	6
not significantly different from	6
of transmissible gastroenteritis of	6
coronaviruses related to transmissible	6
biology of transmissible gastroenteritis	6
in the infected cells	6
sensitivity of the assay	6
of infectious bronchitis virus	6
the jejunum and ileum	6
recombinant s protein was	6
in the cell culture	6
or egfp empty vector	6
the standard deviation for	6
sows that had been	6
of seven novel mammalian	6
negative cells were infected	6
the assembly of the	6
type i interferon production	6
the three commercial elisas	6
the ra plus tgev	6
and then infected with	6
the n protein of	6
identification of porcine transmissible	6
results of this study	6
british field isolate of	6
proportion of cells in	6
carried out as described	6
bat coronaviruses as the	6
directed against virion proteins	6
gene of the prcv	6
differentially expressed proteins were	6
kda expressed by rlppg	6
hp dna transfection reagent	6
and tgev inactivated vaccine	6
as the reciprocal of	6
be explained by the	6
in the process of	6
efficacy and immunogenicity of	6
apn positive cells and	6
engineering the largest rna	6
optical density value obtained	6
based on the transmissible	6
is located in the	6
course of the disease	6
molecular biology of transmissible	6
the nucleocapsid gene of	6
g for min and	6
a domain of aminopeptidase	6
the ectodomain of the	6
and genetic characterization of	6
not present in the	6
in the s and	6
gene source of alphacoronavirus	6
reverse transcription recombinase polymerase	6
removed by centrifugation at	6
the pcr products were	6
by the coronavirus infectious	6
of the carcinoembryonic antigen	6
of alphacoronavirus and betacoronavirus	6
reside within a domain	6
purified tgev as antigen	6
was evaluated by real	6
source of gammacoronavirus and	6
arrest and apoptosis through	6
of the mouse hepatitis	6
the age of the	6
reverse genetics system for	6
and the formation of	6
was performed according to	6
variant porcine epidemic diarrhea	6
intestinal epithelial cells of	6
for the generation of	6
was used for the	6
amino acids of the	6
those in the tgev	6
sulfate polyacrylamide gel electrophoresis	6
the ratio of the	6
the construction of a	6
on the transmissible gastroenteritis	6
of vesicular stomatitis virus	6
tgev and pedv were	6
receptor regulates cofilin activity	6
for a porcine respiratory	6
recombinase polymerase amplification assay	6
were used as the	6
have been developed for	6
of glycoprotein s expressed	6
in the mesenteric lymph	6
to the loss of	6
gastroenteritis virus in vitro	6
were tested for tgev	6
a pair of primers	5
normal goat serum for	5
for the care and	5
is incorporated into virions	5
virus strain pc a	5
immunity against tgev in	5
in the event of	5
removal of sa from	5
are consistent with the	5
amplified by pcr using	5
of the prcv isolates	5
care and use of	5
report of the international	5
infected with transmissible gastroenteritis	5
maintained in maintenance medium	5
can be divided into	5
studies have suggested that	5
changes in the viral	5
to porcine respiratory coronavirus	5
a and l b	5
tgev m protein and	5
important roles in the	5
by western blot analysis	5
of apn and egfr	5
cells transfected with n	5
of the expression of	5
ddx is involved in	5
was obtained from a	5
of the wt group	5
of a coronavirus from	5
declare no conflict of	5
crystal structures of the	5
glycoprotein m affect induction	5
cells were subjected to	5
could significantly increase the	5
clinical illness and better	5
is thought to be	5
against the s protein	5
were compared with the	5
pig kidney cell line	5
with that of the	5
supernatant was collected and	5
the s protein gene	5
shown in table i	5
and is required for	5
a portion of the	5
quantitative pcr and the	5
diarrhea virus and discovery	5
was used as control	5
expressing glycoprotein s polypeptides	5
during the assembly of	5
of a per cent	5
attenuated porcine epidemic diarrhea	5
verified by dna sequencing	5
a leader sequence at	5
and better lactogenic immunity	5
the s gene that	5
used in the present	5
humoral and cellular immune	5
were treated with the	5
of the probiotic bacteria	5
an oral lactobacillus casei	5
epidemic diarrhea in china	5
total rna extracted from	5
the glycoprotein s from	5
st cell monolayers in	5
as well as to	5
partial protection against prrsv	5
this study was to	5
primed as neonates with	5
and mouth disease virus	5
was used to analyze	5
no significant difference between	5
both in vitro and	5
tgev was evaluated by	5
of orf a and	5
virus titers were determined	5
the percentage of sa	5
were euthanized at days	5
has been used for	5
pedv n in the	5
infection induces dramatic changes	5
performed to determine the	5
relationship between tgev and	5
expressed tgev n gene	5
of the innate immune	5
from santa cruz biotechnology	5
pfu ml in ml	5
detection and differentiation of	5
phosphorylation level of egfr	5
of transmissible gastroenteritis on	5
gene counteracts host defenses	5
diarrhea virus and transmissible	5
the highest levels of	5
also been shown to	5
the development of new	5
of apn as a	5
enveloped virus with a	5
the viral replication cycle	5
the viral glycoprotein m	5
this article can be	5
binding to viral determinants	5
evidence for a putative	5
were expressed as the	5
antibodies directed against virion	5
the two parental viruses	5
vector based on the	5
has been demonstrated to	5
was considered statistically significant	5
was more sensitive than	5
extracted using trizol reagent	5
of ibv and mhv	5
restriction fragment length polymorphism	5
incubated in dmem containing	5
respiratory syndrome virus in	5
in the antigenic sites	5
the levels of p	5
the expression level of	5
of transmissible gastroenteritis coronaviruses	5
crystal structure of the	5
of the reproductive tract	5
s domain of the	5
results indicate that egfr	5
has not been established	5
to interact with the	5
be detected in the	5
a membrane protein that	5
gastroenteritis virus shxb isolated	5
gastroenteritis and porcine respiratory	5
were mock infected or	5
the inhibition of tgev	5
role in tgev infection	5
than that observed in	5
a significant increase in	5
of tgev was evaluated	5
tgev in porcine iecs	5
and high mortality in	5
mouse hepatitis virus is	5
gastroenteritis coronavirus nucleocapsid protein	5
the s glycoprotein of	5
the remainder of the	5
piglets were obtained from	5
for human coronavirus e	5
it has also been	5
residues to of the	5
tgev infection induces ifn	5
and ingezim corona diferencial	5
consistent with previous reports	5
pedv and tgev have	5
in the test sample	5
virulent strain of tgev	5
reduced clinical illness and	5
premix ex taq ii	5
a variety of viruses	5
to the end of	5
labeled antibodies for h	5
discovery of a novel	5
affect induction of alpha	5
the exception of the	5
surface of infected cells	5
promoter for tgev entry	5
virus replication in the	5
is the most abundant	5
were removed from the	5
the supernatant was collected	5
recombinant protein expressed in	5
s protein of transmissible	5
that were primed as	5
foot and mouth disease	5
gastroenteritis coronavirus genome is	5
a common feature of	5
antigenically similar to transmissible	5
tgev shaanxi strain was	5
could not be detected	5
impact of transmissible gastroenteritis	5
levels of p and	5
analysis of relative gene	5
of dna and rna	5
the pathogenesis of tgev	5
the cauliflower mosaic virus	5
were primed as neonates	5
nasal shedding of prcv	5
m gene of tgev	5
piglets were inoculated with	5
to the mammary gland	5
presented as the mean	5
depletion from the viral	5
in the immunized group	5
the expression of ifn	5
in each group were	5
time quantitative pcr and	5
that transmissible gastroenteritis virus	5
antibodies containing normal goat	5
similar results were also	5
commercial pig production in	5
for a putative second	5
acid sequences of the	5
neutralizing antibody titers were	5
fecal samples were collected	5
nucleotide and amino acid	5
porcine small intestine epithelial	5
outbreaks of diarrhea in	5
to be associated with	5
development and evaluation of	5
proteolytic cleavage of the	5
better lactogenic immunity against	5
the expression of il	5
growth factor receptor is	5
cells expressed tgev n	5
dmem containing dispase ii	5
nested polymerase chain reaction	5
expression data using real	5
prevalence of porcine epidemic	5
molecular basis of transmissible	5
increase the number of	5
in gilts that were	5
the concentration of the	5
were used to evaluate	5
both pcv and tgev	5
second receptor for porcine	5
proteomic analysis reveals that	5
the fusion of the	5
damage via targeting rb	5
virus infection induces dramatic	5
limit of the method	5
cells of the small	5
lactic acid bacteria can	5
mouse hepatitis virus and	5
was prepared in our	5
cellular receptor for tgev	5
results indicate that the	5
the enteric tropism determinant	5
domain of glycoprotein s	5
st cells were synchronized	5
egfp expression vector or	5
of each sample was	5
that of the wt	5
western blot analysis of	5
with tgev strain miller	5
m and n genes	5
membrane protein that is	5
was kindly provided by	5
piglets were challenged with	5
to the absence of	5
salmonella enterica serovar typhimurium	5
not appear to be	5
kits evaluated in this	5
h prior to infection	5
for the construction of	5
paraformaldehyde for min at	5
for the preparation of	5
morbidity and mortality in	5
this type of di	5
than that of conventional	5
by the indirect fluorescent	5
a highly contagious disease	5
induces apoptosis through fasl	5
of monoclonal antibodies directed	5
shxb isolated in china	5
compared with the pbs	5
has not been elucidated	5
plays a crucial role	5
not the related porcine	5
the carcinoembryonic antigen family	5
gastroenteritis on commercial pig	5
the prcv s protein	5
morphogenesis and proliferative rule	5
of protective lactogenic immunity	5
the immune response of	5
shedding of prcv tgev	5
responsible for receptor binding	5
were blocked for h	5
in apn negative cells	5
the s gene from	5
and washed with pbs	5
s group was significantly	5
prevention and control of	5
m and n proteins	5
have not yet been	5
to a lesser extent	5
recent studies have shown	5
in the level of	5
s b and table	5
at seven days cultivation	5
terminal domain of spike	5
epidemic diarrhea virus into	5
with any of the	5
the potential role of	5
receptor porcine aminopeptidase n	5
using monoclonal antibodies to	5
for min and then	5
the crystal structure of	5
reported that tgev infection	5
in the natural host	5
that observed in the	5
characteristics of a coronavirus	5
coronavirus gene counteracts host	5
piglets born to vaccinated	5
the antiviral activity of	5
previous studies showed that	5
in the form of	5
the activation of egfr	5
was carried out with	5
a high level of	5
j cells were seeded	5
were identical to those	5
were washed five times	5
as shown in figures	5
sequence comparison of porcine	5
pcr was performed on	5
stat in the lp	5
lactic acid bacteria nc	5
before and after nebulization	5
isolate of transmissible gastroenteritis	5
b cells and cd	5
of a reverse transcription	5
the binding site of	5
modified live virus vaccine	5
the intensity of the	5
site of the tgev	5
moi for h at	5
the results of three	5
mab to n protein	5
the tip of the	5
quantitative proteomic analysis reveals	5
for the detection and	5
the presence of tunicamycin	5
encoding the s protein	5
supplemented with fbs and	5
s gene from the	5
from transmissible gastroenteritis coronavirus	5
were harvested at the	5
see materials and methods	5
in the viral glycoprotein	5
is not clear whether	5
attenuated variant of transmissible	5
in the dko group	5
cells transfected with the	5
mrna and protein levels	5
the prevention and control	5
the results show that	5
the fact that the	5
to test this hypothesis	5
synergistically promote tgev invasion	5
protein of tgev has	5
neutralizing antibodies against the	5
with porcine epidemic diarrhea	5
play a key role	5
swine enteric coronavirus from	5
and modulates virus virulence	5
the results indicate that	5
length infectious cdna clone	5
simulation of the economic	5
of type i interferon	5
cells were harvested at	5
cells were used for	5
and its role in	5
mt d in ml	5
mitochondrial damage via targeting	5
in animals immunized with	5
designed to amplify the	5
the recombinant lactobacillus strain	5
porcine epidemic diarrhoea in	5
virulent strain of transmissible	5
deficient virus vector based	5
a polypeptide of m	5
washed five times with	5
the tgev group at	5
expression levels of zap	5
to determine whether tgev	5
junctions and microfilaments of	5
in dmem containing dispase	5
the stability of the	5
diarrhoea virus and transmissible	5
diarrhoea virus infection induces	5
of the sars coronavirus	5
the villi to the	5
in the splenic lymphocytes	5
shedding of porcine respiratory	5
by inserting the oligo	5
or g m phase	5
amount of phosphorylated stat	5
the use of the	5
of the heterologous gene	5
to human rotavirus in	5
relative gene expression data	5
results showed that nc	5
genomic sequence of the	5
with tgev strain purdue	5
tremegene hp dna transfection	5
arranged in the order	5
is essential for the	5
were used to determine	5
the target of mir	5
changes in the tight	5
tgev entry in a	5
in the secretory pathway	5
the levels of viral	5
as neonates with porcine	5
involved in the induction	5
of swine enteric coronaviruses	5
t g and pbs	5
of a novel swine	5
in the concentration of	5
twice with pbs and	5
and entry of tgev	5
national center for biotechnology	5
of the tgev group	5
article can be found	5
in the replication of	5
the limit of detection	5
basis of transmissible gastroenteritis	5
the s proteins from	5
the epitopes recognized by	5
were performed in triplicate	5
the importance of cholesterol	5
induction of cell cycle	5
of sglt and glut	5
nucleocapsid protein of transmissible	5
cell supernatants were harvested	5
of the presence of	5
is similar to that	5
m affect induction of	5
of hepatitis c virus	5
was not observed in	5
the length of the	5
in escherichia coli and	5
gene expression data using	5
cells were then incubated	5
recombinant swine enteric coronavirus	5
a and d in	5
tgev infection caused the	5
domain complexed with its	5
as described in the	5
s a and table	5
differences were considered significant	5
is expressed in the	5
the increased concentration of	5
viral glycoprotein m affect	5
involved in the antigenic	5
u m a n	5
the tgev n gene	5
tropism of the virus	5
n protein expressed pk	5
the he protein of	5
the major structural proteins	5
fipv type i viruses	5
immunoprecipitation of glycoprotein s	5
epidemic diarrhoea virus infection	5
adapted to grow in	5
results were analyzed by	5
with neutralizing activity against	5
in the coronaviridae family	5
translation of orf b	5
the majority of the	5
deletion in the s	5
to days after infection	5
increased concentration of il	5
an enveloped virus with	5
were fixed in absolute	5
has been used to	5
increased litter survival rates	5
be due to the	5
of iga b cells	5
been identified as a	5
and processing of the	5
and the results were	5
it is known that	5
separated proteins were transferred	5
virulent british field isolate	5
used as templates for	5
induces dramatic changes in	5
a virulent british field	5
pigs of the same	5
nucleocapsid protein induces cell	5
tight junctions and microfilaments	5
and functional characterization of	5
the mortality rate of	5
predicted amino acid sequences	5
in pedv and tgev	5
and their ability to	5
developed in this study	5
and rna viruses in	5
protein that is incorporated	5
used to determine the	5
modified live tgev vaccine	5
and macrophage cell lines	5
peripheral blood mononuclear cells	5
coronavirus nucleocapsid protein gene	5
half of the s	5
similar results were obtained	5
porcine respiratory coronavirus and	5
propagation of the virus	5
previous studies have found	5
authors declare no conflict	5
used to analyze the	5
was performed in triplicate	5
the best of our	5
an infectious cdna clone	5
expression vector or egfp	5
of enteric viral infections	5
challenge with transmissible gastroenteritis	5
as a template for	5
expression levels of the	5
derived from the anpep	5
weaned pigs and sequence	5
virus shxb isolated in	5
pbs and lysed in	5
host innate immune response	5
promoter luciferase report plasmids	5
for the differentiation of	5
the data shown are	5
for cv fecal suspension	5
with pbs and fixed	5
ability of the virus	5
expression levels in the	5
on commercial pig production	5
chain reaction assay for	5
read and approved the	5
receptor determinants for coronaviruses	5
the age of days	5
inoculation of sows with	5
between prcv and tgev	5
debris was removed by	5
the expression of p	5
well culture chamber slides	5
is believed to be	5
fixed in absolute acetone	5
h u m a	5
receptor for porcine transmissible	5
antigenic sites of transmissible	5
differentiation between transmissible gastroenteritis	5
cells were incubated for	5
outbreak of porcine epidemic	5
activating p mapk pathway	5
a mouse hepatitis virus	5
the cell culture medium	5
bca protein assay kit	5
van gorp et al	5
the care and use	5
of the virus and	5
of swine and a	5
were purchased from sigma	5
of the sa protein	5
cloned into the same	5
the related porcine respiratory	5
the structural proteins of	5
discovery of a recombinant	5
related group was comprised	5
of the s domain	5
the presence of glucose	5
coronavirus spike protein is	5
infected st cells was	5
was resuspended in ml	5
the leader rna of	5
cellular proteins were identified	5
experiment was performed in	5
were expressed as means	5
of the m gene	5
tgev infection in pk	5
were interpreted as negative	5
differences were found between	5
entry in a dose	5
due to the absence	5
among transmissible gastroenteritis virus	5
tgev and prcv is	5
transmissible gastroenteritis virus the	5
times to remove unbound	5
at the nonpermissive temperature	5
collected by centrifugation at	5
virus vector based on	5
in the tight junctions	5
the respiratory activity of	5
the present study was	5
the transcription start site	5
tgev infection induced the	5
agent of transmissible gastroenteritis	5
at the plasma membrane	5
was extracted using trizol	5
evidence for a porcine	5
virulent rv inoculated pigs	5
in well culture chamber	5
has also been demonstrated	5
were stained with antibodies	5
against tgev in gilts	5
observed in the animals	5
transmissible gastroenteritis coronavirus nucleocapsid	5
diarrhea virus strain pc	5
for both tgev and	5
in roswell park memorial	5
of freezing and thawing	5
a recombinant vaccinia virus	5
the murine coronavirus spike	5
virus and discovery of	5
a large deletion in	5
in mice orally immunized	5
in vitro inhibition of	5
infection of pigs with	5
attached to the cells	5
cells in the s	5
as a loading control	5
of the dko pigs	5
were orally inoculated with	5
were fixed in paraformaldehyde	5
like particles associated with	5
sequence of the coronavirus	5
the g m phase	5
economic impact of transmissible	5
compared to the control	5
which is consistent with	5
as the log of	5
had been inoculated with	5
characterization of a coronavirus	5
the late stage of	5
antibody responses in serum	5
development of a reverse	5
was isolated from an	5
hydrophobic protein encoded at	5
in transmissible gastroenteritis of	5
at the level of	5
illness and better lactogenic	5
receptor for human coronavirus	5
to the best of	5
comparison of porcine respiratory	5
host defenses and modulates	5
was not isolated from	5
a against tgev replication	5
of a recombinant swine	5
it is also possible	5
the transcription of tgev	5
pigs and sequence of	5
antigenic differentiation between transmissible	5
and mesenteric lymph node	5
as receptor determinants for	5
used to amplify the	5
were obtained from the	5
appl microbiol biotechnol doi	5
the virulent strain of	5
and incubated for min	5
the time of farrowing	5
transmissible gastroenteritis of pigs	5
of viral host range	5
higher than those in	5
were verified by dna	5
properties of monoclonal antibodies	5
p infected cell lysates	5
in the cells transfected	5
the phosphorylation of p	5
m and s antigens	5
transfected with p sirna	5
tgev nucleocapsid protein induces	5
the large size of	5
of this study was	5
determinant was expressed as	5
transmissible gastroenteritis virus molecular	5
greater than that of	5
specified by the coronavirus	5
of tgev in porcine	5
each of the three	5
of relative gene expression	5
pedv and rotavirus a	5
acids as receptor determinants	5
sialic acids as receptor	5
for porcine transmissible gastroenteritis	5
different from that of	5
gastroenteritis virus of pigs	5
cells were then washed	5
pcr was performed using	5
encodes a membrane protein	5
fecal shedding of porcine	5
were transfected with p	5
of the coronavirus genome	5
transmissible gastroenteritis virus shxb	5
cauliflower mosaic virus s	5
fitc labeled antibodies for	5
in this paper we	5
the villi of the	5
the university of minnesota	5
in order to test	5
is related to the	5
end of the s	5
in the current study	5
large deletion in the	5
was provided by dr	5
sem of three independent	5
the possibility that the	5
days after the last	5
interaction between tgev s	5
orf encodes a membrane	5
h and h of	5
compared with the tgev	5
sentinel weaned pigs and	5
antibody binding to viral	5
transmissible gastroenteritis on commercial	5
of the subgenomic mrnas	5
protein is the most	5
the porcine aminopeptidase n	5
the cd and papn	5
the mechanism by which	5
tgev in gilts that	5
and position of the	5
between virulent and attenuated	5
study is the first	5
was supported by grants	5
variant of transmissible gastroenteritis	5
no deletions or insertions	5
that apn is not	5
has a sialic acid	5
by grants from the	5
la monica et al	5
in pbs for h	5
by centrifugation at g	5
mouse hepatitis virus a	5
porcine coronavirus transmissible gastroenteritis	5
the role of the	5
have been identified in	5
is required for tgev	5
was determined using a	5
were performed using the	5
in sentinel weaned pigs	5
was performed using a	5
that the e protein	5
a better understanding of	5
it has been proposed	5
to the development of	5
in the i i	5
having specificity for the	5
of the gastrointestinal tract	5
for an additional h	5
is also possible that	5
dilution was added to	5
neonates with porcine respiratory	5
the detection of pedv	5
harvested at the indicated	5
for the avian coronavirus	5
evaluated in this study	5
sequence of the partial	5
a co atmosphere incubator	5
on the detection of	5
t cells were cultured	5
extracellular receptor binding domain	5
and discovery of a	5
three times to remove	5
a and table s	5
and porcine hemagglutinating encephalomyelitis	5
were considered significant at	5
microfilaments of polarized ipec	5
the united states and	5
e protein is essential	5
normalized to control conditions	5