This is a table of type bigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.
bigram | frequency |
---|---|
transmissible gastroenteritis | 1113 |
gastroenteritis virus | 725 |
tgev infection | 503 |
porcine epidemic | 351 |
infected cells | 335 |
epidemic diarrhea | 308 |
diarrhea virus | 265 |
st cells | 260 |
epithelial cells | 238 |
porcine respiratory | 223 |
respiratory coronavirus | 204 |
gastroenteritis coronavirus | 203 |
cell culture | 191 |
respiratory syndrome | 179 |
amino acid | 175 |
spike protein | 172 |
hepatitis virus | 170 |
immune responses | 164 |
mouse hepatitis | 152 |
cell cycle | 151 |
virus infection | 149 |
immune response | 142 |
cell line | 134 |
neutralizing antibodies | 134 |
cell lines | 128 |
viral rna | 125 |
porcine transmissible | 114 |
rna synthesis | 108 |
small intestine | 107 |
three times | 107 |
structural proteins | 106 |
leader rna | 106 |
genomic rna | 105 |
doc id | 103 |
intestinal epithelial | 103 |
infectious bronchitis | 103 |
cells infected | 103 |
cord uid | 103 |
monoclonal antibodies | 103 |
cell surface | 100 |
clinical signs | 99 |
time rt | 99 |
porcine reproductive | 97 |
results showed | 96 |
bronchitis virus | 95 |
infectious peritonitis | 93 |
tgev replication | 93 |
feline infectious | 92 |
syndrome virus | 90 |
room temperature | 90 |
viral replication | 88 |
expression levels | 87 |
nucleocapsid protein | 87 |
virus replication | 87 |
amino acids | 86 |
goat anti | 86 |
di rna | 84 |
viral infection | 83 |
murine coronavirus | 82 |
western blot | 82 |
tgev strains | 79 |
tissue culture | 78 |
swine enteric | 77 |
enteric tropism | 75 |
tgev strain | 74 |
antigenic sites | 74 |
sialic acid | 73 |
membrane protein | 72 |
present study | 71 |
time pcr | 71 |
tgev prcv | 70 |
type i | 70 |
pcr assay | 70 |
previously described | 69 |
bovine serum | 69 |
severe acute | 68 |
strand rna | 68 |
virus particles | 68 |
well plates | 68 |
newborn piglets | 67 |
acute respiratory | 67 |
signaling pathway | 67 |
lactogenic immunity | 67 |
cell membrane | 66 |
western blotting | 65 |
important role | 65 |
reverse transcription | 65 |
human coronavirus | 64 |
enteric coronavirus | 64 |
significantly higher | 63 |
virus entry | 62 |
tgev pl | 62 |
mucosal immune | 62 |
negative control | 62 |
mrna species | 61 |
dko pigs | 61 |
sequence analysis | 61 |
monoclonal antibody | 61 |
total rna | 61 |
open reading | 60 |
host cell | 60 |
protein expression | 59 |
cycle arrest | 59 |
tgev entry | 59 |
performed using | 58 |
neutralizing antibody | 58 |
rabbit anti | 58 |
respiratory tract | 58 |
cell cultures | 57 |
rna viruses | 57 |
control group | 57 |
cell lysates | 57 |
glucose uptake | 57 |
porcine deltacoronavirus | 56 |
united states | 56 |
previous studies | 56 |
pfu ml | 55 |
host cells | 55 |
culture medium | 55 |
immune system | 54 |
bovine coronavirus | 54 |
rna genome | 54 |
epithelial cell | 54 |
electron microscopy | 54 |
differentially expressed | 54 |
peritonitis virus | 54 |
escherichia coli | 54 |
serum samples | 53 |
transgenic plants | 53 |
mg ml | 52 |
subgenomic mrnas | 52 |
washed three | 52 |
antibody titers | 51 |
spedv virus | 51 |
influenza virus | 51 |
three independent | 51 |
induced apoptosis | 51 |
syndrome coronavirus | 51 |
days post | 51 |
virus strains | 50 |
coronavirus spike | 50 |
recombinant lactobacillus | 49 |
described previously | 49 |
gnotobiotic pigs | 48 |
epidemic diarrhoea | 48 |
leader sequence | 48 |
gene expression | 47 |
rna polymerase | 47 |
intestinal contents | 47 |
nucleotide sequence | 46 |
viral proteins | 46 |
vero cells | 46 |
old piglets | 46 |
like particles | 46 |
enteric coronaviruses | 46 |
per well | 46 |
tgev genome | 45 |
buffered saline | 45 |
cellular receptor | 45 |
closely related | 45 |
terminal domain | 45 |
disease virus | 44 |
gp fr | 44 |
innate immune | 44 |
independent experiments | 44 |
receptor binding | 44 |
virus strain | 44 |
cell entry | 44 |
reading frame | 44 |
il rap | 43 |
growth factor | 43 |
coronavirus infection | 43 |
antibody binding | 43 |
plasma membrane | 43 |
maternal antibodies | 42 |
specific antibodies | 42 |
suckling piglets | 42 |
viral genome | 42 |
determine whether | 41 |
antibody responses | 41 |
economic losses | 41 |
antibody response | 41 |
porcine intestinal | 41 |
genome sequence | 41 |
pedv infection | 41 |
lactic acid | 41 |
tgev miller | 41 |
cellular proteins | 41 |
complete genome | 41 |
flow cytometry | 41 |
tgev group | 41 |
tgev purdue | 41 |
passive immunity | 40 |
oral immunization | 40 |
prcv strains | 40 |
dendritic cells | 40 |
cell fusion | 40 |
porcine coronavirus | 40 |
porcine aminopeptidase | 40 |
infection induces | 40 |
rna replication | 40 |
viral protein | 39 |
pcr products | 39 |
paneth cells | 39 |
infected piglets | 39 |
structural protein | 39 |
cell proliferation | 39 |
results suggest | 39 |
lysis buffer | 39 |
acid bacteria | 39 |
orf gene | 38 |
binding activity | 38 |
rabbit igg | 38 |
virus binding | 38 |
binding domain | 38 |
modified live | 38 |
spike glycoprotein | 38 |
may also | 38 |
duplex undp | 38 |
results indicate | 38 |
copies ml | 38 |
mucosal immunity | 38 |
antigenic relationship | 37 |
coronavirus transmissible | 37 |
target cells | 37 |
cells treated | 37 |
cells expressing | 37 |
spike gene | 37 |
high levels | 37 |
type ii | 37 |
wt pigs | 37 |
fetal bovine | 37 |
viral antigens | 37 |
tgev infected | 36 |
taken together | 36 |
conjugated goat | 36 |
protein expressed | 36 |
significantly increased | 36 |
cells transfected | 36 |
virus titers | 36 |
nucleoprotein gene | 36 |
virulent tgev | 36 |
lamina propria | 36 |
villous enterocytes | 36 |
time points | 36 |
tgev spike | 36 |
lgr iscs | 36 |
infected st | 36 |
tgev antigen | 36 |
per cent | 35 |
reverse genetics | 35 |
rna recombination | 35 |
stranded rna | 35 |
positive cells | 35 |
molecular biology | 35 |
expression vector | 35 |
fusion protein | 35 |
blot analysis | 35 |
encephalomyelitis virus | 34 |
neonatal piglets | 34 |
intestinal epithelium | 34 |
signaling pathways | 34 |
vaccine candidate | 34 |
gold nanoparticles | 34 |
diarrhoea virus | 34 |
pdcov infection | 34 |
tgev particles | 34 |
infectious virus | 34 |
thermo scientific | 34 |
binding site | 34 |
rna virus | 33 |
vaccinated animals | 33 |
villous atrophy | 33 |
virus neutralization | 33 |
blocking elisa | 33 |
purdue strain | 33 |
mortality rate | 33 |
di rnas | 33 |
coronavirus nucleocapsid | 33 |
vaccinia virus | 32 |
endoplasmic reticulum | 32 |
lipid rafts | 32 |
subgenomic mrna | 32 |
small intestinal | 32 |
tgev rna | 32 |
cell apoptosis | 32 |
results indicated | 32 |
rapid detection | 32 |
protein levels | 32 |
gel electrophoresis | 32 |
family coronaviridae | 32 |
coronavirus genome | 32 |
epidermal growth | 32 |
virus isolation | 31 |
rna sequence | 31 |
fecal samples | 31 |
cytopathic effect | 31 |
multiplex real | 31 |
two different | 31 |
pregnant sows | 31 |
binding sites | 31 |
mrna transcription | 31 |
primary antibodies | 30 |
equine influenza | 30 |
tcid ml | 30 |
high mortality | 30 |
significant difference | 30 |
membrane fusion | 30 |
mitochondrial damage | 30 |
infectious cdna | 30 |
nonstructural protein | 30 |
viral genomic | 30 |
secondary antibody | 30 |
polyacrylamide gel | 30 |
protective immunity | 30 |
canine coronavirus | 30 |
immunized animals | 29 |
plaque assay | 29 |
recombinant viruses | 29 |
stem cells | 29 |
infected group | 29 |
acid binding | 29 |
previous study | 29 |
tgev gene | 29 |
heterologous gene | 29 |
porcine enteric | 29 |
washed twice | 29 |
medium containing | 29 |
calf serum | 29 |
mrna synthesis | 28 |
virus genome | 28 |
analyzed using | 28 |
fixed cells | 28 |
major antigenic | 28 |
acid sequence | 28 |
acid changes | 28 |
virus attachment | 28 |
secondary antibodies | 28 |
protein gene | 28 |
factor receptor | 28 |
colon epithelial | 28 |
hemagglutinating encephalomyelitis | 28 |
infected pk | 28 |
golgi complex | 28 |
virus titer | 28 |
st cell | 27 |
cdna clone | 27 |
tgev antibodies | 27 |
sa protein | 27 |
post infection | 27 |
expression systems | 27 |
nucleic acid | 27 |
human rotavirus | 27 |
protein genes | 27 |
statistically significant | 27 |
infectious clone | 27 |
reading frames | 27 |
coronavirus infectious | 27 |
notch signaling | 27 |
avian infectious | 27 |
lactobacillus plantarum | 27 |
watery diarrhea | 27 |
significant differences | 27 |
coronavirus replication | 27 |
fluorescence microscopy | 27 |
cell types | 27 |
virulent pedv | 27 |
porcine coronaviruses | 27 |
iga antibodies | 26 |
copy number | 26 |
virus type | 26 |
coronavirus tgev | 26 |
cell viability | 26 |
gfp bac | 26 |
rna sequences | 26 |
infected cell | 26 |
magnetic beads | 26 |
specific antibody | 26 |
swine transmissible | 26 |
induced cell | 26 |
additional file | 26 |
pedv strain | 26 |
mouse igg | 26 |
pedv replication | 26 |
pcr method | 26 |
linked immunosorbent | 26 |
authors declare | 26 |
neutralising antibody | 26 |
standard deviation | 26 |
severe diarrhea | 26 |
mouse monoclonal | 26 |
pedv strains | 25 |
tgev rbd | 25 |
rna transcription | 25 |
cholesterol depletion | 25 |
tissue tropism | 25 |
three days | 25 |
fetal calf | 25 |
mrna expression | 25 |
mouse anti | 25 |
coronavirus infections | 25 |
horseradish peroxidase | 25 |
similar results | 25 |
sodium dodecyl | 25 |
protein synthesis | 25 |
cdna clones | 25 |
acid sequences | 25 |
murine hepatitis | 25 |
pigs inoculated | 25 |
apn positive | 25 |
suspect results | 25 |
tgev nucleoprotein | 25 |
neonatal pigs | 25 |
primary enterocytes | 25 |
kidney cells | 25 |
porcine kidney | 25 |
clinical symptoms | 25 |
enteric disease | 25 |
data indicate | 25 |
i interferon | 25 |
purified tgev | 25 |
cells per | 25 |
cd ssc | 24 |
negative cells | 24 |
helper virus | 24 |
viral infections | 24 |
ileum epithelial | 24 |
enveloped viruses | 24 |
oral vaccine | 24 |
viral antigen | 24 |
recombinant virus | 24 |
herpes simplex | 24 |
major receptor | 24 |
swine testis | 24 |
live virus | 24 |
immunosorbent assay | 24 |
veroe cells | 24 |
mesenteric lymph | 24 |
probiotic bacteria | 24 |
respiratory disease | 24 |
vesicular stomatitis | 24 |
coronaviridae family | 24 |
restriction sites | 24 |
cd cells | 24 |
antiviral activity | 24 |
targeted recombination | 24 |
envelope protein | 24 |
infected ipec | 24 |
significantly different | 24 |
rotavirus infection | 24 |
lab strains | 24 |
coronavirus rna | 24 |
newborn pigs | 24 |
multiplex rt | 23 |
tgev vaccine | 23 |
trizol reagent | 23 |
specific primers | 23 |
restriction endonuclease | 23 |
empty vector | 23 |
different time | 23 |
clinical disease | 23 |
crystal structure | 23 |
sense rna | 23 |
significantly lower | 23 |
oral administration | 23 |
ingezim corona | 23 |
luciferase reporter | 23 |
lymph nodes | 23 |
viral envelope | 23 |
kda protein | 23 |
retinoic acid | 23 |
virulent strain | 23 |
tgev invasion | 23 |
lactobacillus casei | 23 |
coronavirus mouse | 23 |
serum albumin | 23 |
inflammatory response | 23 |
stomatitis virus | 23 |
prcv strain | 23 |
related porcine | 23 |
sars coronavirus | 23 |
anpep ko | 22 |
apn negative | 22 |
rna interference | 22 |
recombinant plasmids | 22 |
peplomer protein | 22 |
recent studies | 22 |
study showed | 22 |
human cytomegalovirus | 22 |
mediated endocytosis | 22 |
interfering rna | 22 |
fluorescent antibody | 22 |
highly conserved | 22 |
recombinant plasmid | 22 |
genbank accession | 22 |
highly contagious | 22 |
antigenically related | 22 |
porcine enterocytes | 22 |
dodecyl sulfate | 22 |
porcine rotavirus | 22 |
secreting cells | 22 |
phage display | 22 |
phylogenetic analysis | 22 |
indirect immunofluorescence | 22 |
confocal microscopy | 22 |
surface antigen | 22 |
cells compared | 22 |
santa cruz | 22 |
attenuated tgev | 21 |
passive protection | 21 |
induced ifn | 21 |
suckling pigs | 21 |
two types | 21 |
may play | 21 |
igg antibody | 21 |
cell responses | 21 |
corona diferencial | 21 |
lymphoid tissues | 21 |
antigenic site | 21 |
tgev neutralizing | 21 |
molecular weight | 21 |
gene sequences | 21 |
gene encoding | 21 |
strand rnas | 21 |
detected using | 21 |
coronavirus mhv | 21 |
nasal swabs | 21 |
length cdna | 21 |
tge virus | 21 |
rna extraction | 21 |
infected pigs | 21 |
enteric viruses | 21 |
subgenomic negative | 21 |
significantly reduced | 21 |
cell monolayers | 21 |
viral particles | 21 |
cells using | 21 |
previously reported | 21 |
optical density | 20 |
enteric pathogens | 20 |
virus spike | 20 |
enteropathogenic coronavirus | 20 |
ko pigs | 20 |
serially diluted | 20 |
prcv isolates | 20 |
pigs infected | 20 |
pcr using | 20 |
immunofluorescence staining | 20 |
dependent rna | 20 |
protein level | 20 |
chain reaction | 20 |
acute diarrhea | 20 |
virus vaccine | 20 |
lactobacillus vaccine | 20 |
primary porcine | 20 |
major structural | 20 |
conjugated secondary | 20 |
whole virus | 20 |
recombinant protein | 20 |
polymerase chain | 20 |
mm tris | 20 |
functional receptor | 20 |
fipv type | 20 |
culture adapted | 20 |
surface projections | 20 |
gene sequence | 20 |
simplex virus | 20 |
porcine iecs | 20 |
swine testicle | 20 |
prrsv infection | 20 |
induces cell | 20 |
mrna start | 19 |
fluorescence microscope | 19 |
expression plasmid | 19 |
fold higher | 19 |
integral membrane | 19 |
host range | 19 |
human immunodeficiency | 19 |
heterologous genes | 19 |
pcr amplification | 19 |
whether tgev | 19 |
experimentally infected | 19 |
gp ep | 19 |
iga antibody | 19 |
van der | 19 |
stably expressing | 19 |
actin cytoskeleton | 19 |
probably due | 19 |
infected animals | 19 |
experimental infection | 19 |
viral titers | 19 |
agarose gel | 19 |
also observed | 19 |
swine industry | 19 |
infection induced | 19 |
miller strain | 19 |
seven days | 19 |
af mab | 19 |
virus rna | 19 |
pig kidney | 19 |
orally administered | 19 |
mouth disease | 19 |
coronavirus related | 19 |
determined using | 19 |
three groups | 19 |
ng ml | 19 |
cell death | 19 |
health care | 19 |
log reduction | 19 |
green fluorescence | 19 |
like receptor | 19 |
treated cells | 19 |
fc receptor | 19 |
four major | 19 |
pig farms | 19 |
serum antibodies | 19 |
discontinuous transcription | 19 |
specific inhibitor | 19 |
cultured cells | 19 |
immunodeficiency virus | 18 |
point mutations | 18 |
clinical samples | 18 |
survival rate | 18 |
goblet cells | 18 |
life cycle | 18 |
calculated using | 18 |
also found | 18 |
like protease | 18 |
table i | 18 |
coronavirus gene | 18 |
packaging signal | 18 |
lymph node | 18 |
viral membrane | 18 |
dmem containing | 18 |
faecal samples | 18 |
serum sample | 18 |
two groups | 18 |
tgev orf | 18 |
molecular basis | 18 |
detection limit | 18 |
porcine circovirus | 18 |
pfcrn expression | 18 |
incubation period | 18 |
field strains | 18 |
apoptosis via | 18 |
carcinoembryonic antigen | 18 |
two viruses | 18 |
three major | 18 |
serum igg | 18 |
antiviral effect | 18 |
protein kinase | 18 |
gastrointestinal tract | 18 |
mapk pathway | 18 |
stat signaling | 18 |
promoter activity | 18 |
gp ecto | 18 |
prcv tgev | 18 |
located within | 18 |
pcr analysis | 18 |
recombinant vaccinia | 18 |
host interactions | 18 |
pbs containing | 18 |
coronavirus ibv | 18 |
important roles | 18 |
dependent manner | 18 |
terminal half | 18 |
consensus sequence | 18 |
papn ectodomain | 17 |
apn expression | 17 |
pfcrn gene | 17 |
pedv virus | 17 |
innate immunity | 17 |
polyclonal antibody | 17 |
subunit vaccines | 17 |
recombinant tgev | 17 |
elisa kit | 17 |
virus assembly | 17 |
diferencial elisa | 17 |
virus production | 17 |
positive control | 17 |
enterococcus faecium | 17 |
lg ml | 17 |
protease inhibitor | 17 |
fecal shedding | 17 |
gp protein | 17 |
swinepox virus | 17 |
results suggested | 17 |
inhibitory effect | 17 |
transcription factor | 17 |
feline coronavirus | 17 |
specific binding | 17 |
tgev serum | 17 |
rabbit polyclonal | 17 |
ms ms | 17 |
orally immunized | 17 |
cellular immunity | 17 |
enteric viral | 17 |
like agent | 17 |
results obtained | 17 |
serum antibody | 17 |
uninfected cells | 17 |
fecal suspension | 17 |
pcr product | 17 |
previous reports | 17 |
plasmid dna | 17 |
svanovir tgev | 17 |
control cells | 17 |
mammalian cells | 17 |
live attenuated | 17 |
transfected cells | 17 |
homing receptors | 17 |
rna helicase | 17 |
based real | 17 |
sentinel pigs | 17 |
reverse primer | 17 |
start site | 17 |
results demonstrated | 17 |
dna sequencing | 17 |
weaned pigs | 17 |
commercially available | 17 |
high level | 17 |
inactivated virus | 17 |
polymerase gene | 16 |
vero adapted | 16 |
crispr cas | 16 |
renilla luciferase | 16 |
systemic immune | 16 |
alveolar macrophages | 16 |
attenuated strains | 16 |
bound tgev | 16 |
essential medium | 16 |
terminal region | 16 |
different concentrations | 16 |
protein interactions | 16 |
induced mitochondrial | 16 |
two amino | 16 |
three commercial | 16 |
intestinal cells | 16 |
cycle progression | 16 |
mrna levels | 16 |
forward primer | 16 |
culture supernatants | 16 |
secondary structure | 16 |
data suggest | 16 |
sialic acids | 16 |
strain purdue | 16 |
genomic sequences | 16 |
weaned piglets | 16 |
reverse transcriptase | 16 |
partial protection | 16 |
protein induces | 16 |
internal entry | 16 |
porcine apn | 16 |
live vaccine | 16 |
virion assembly | 16 |
mm nacl | 16 |
viral challenge | 16 |
protein may | 16 |
infected bhk | 16 |
defective interfering | 16 |
avian coronaviruses | 16 |
co incubator | 16 |
statistical analysis | 16 |
cell growth | 16 |
causative agent | 16 |
results show | 16 |
indirect fluorescent | 16 |
even though | 16 |
cellular receptors | 16 |
tyrosine kinase | 16 |
yeast cells | 16 |
well plate | 16 |
high specificity | 16 |
widely used | 16 |
transferrin receptor | 16 |
inoculated pigs | 16 |
electron microscope | 16 |
intestinal stem | 16 |
foreign genes | 16 |
canine parvovirus | 16 |
tgev virions | 16 |
tgev challenge | 16 |
largest rna | 16 |
cell signaling | 16 |
entry receptor | 16 |
human coronaviruses | 16 |
signal peptide | 16 |
expressed lncrnas | 16 |
monoclonal anti | 16 |
associated lymphoid | 16 |
like cells | 16 |
variant tgev | 16 |
measured using | 16 |
expression level | 16 |
swine herds | 16 |
life technologies | 16 |
naturally occurring | 15 |
polyclonal antibodies | 15 |
antibody levels | 15 |
accession number | 15 |
oral corn | 15 |
positive samples | 15 |
buffer containing | 15 |
simultaneous detection | 15 |
live vaccines | 15 |
protein assay | 15 |
neutralizing epitopes | 15 |
culture supernatant | 15 |
proteomic analysis | 15 |
expressed proteins | 15 |
animals immunized | 15 |
total protein | 15 |
analysis using | 15 |
expression system | 15 |
indirect elisa | 15 |
based vaccine | 15 |
gene editing | 15 |
wild type | 15 |
science foundation | 15 |
systemic immunity | 15 |
isothermal amplification | 15 |
coronavirus mrnas | 15 |
virulent rv | 15 |
ml penicillin | 15 |
viral shedding | 15 |
specific mabs | 15 |
infected jejunum | 15 |
pg ml | 15 |
enteric covs | 15 |
humoral immune | 15 |
based vectors | 15 |
via targeting | 15 |
using monoclonal | 15 |
play important | 15 |
knockout pigs | 15 |
us pedv | 15 |
virus infectivity | 15 |
murine coronaviruses | 15 |
gnotobiotic pig | 15 |
kindly provided | 15 |
diagnostic sensitivity | 15 |
transmission electron | 15 |
weeks old | 15 |
phosphate buffered | 15 |
mortality rates | 15 |
antigenic structure | 15 |
cd cd | 15 |
virus envelope | 15 |
recombinant ad | 15 |
molecular characterization | 15 |
nuclear translocation | 15 |
leaky scanning | 15 |
luciferase activity | 15 |
expression vectors | 15 |
igg antibodies | 15 |
mm edta | 15 |
group coronaviruses | 15 |
surface glycoprotein | 15 |
analysis showed | 15 |
elisa kits | 15 |
expressing tgev | 15 |
class i | 15 |
virus vaccines | 15 |
ab elisa | 15 |
border membrane | 15 |
pedv cv | 15 |
cells stably | 15 |
genetically engineered | 15 |
parental viruses | 15 |
responses induced | 14 |
dmem supplemented | 14 |
egfr receptor | 14 |
considered significant | 14 |
calf diarrhea | 14 |
pk cells | 14 |
molecular cloning | 14 |
tgev vaccines | 14 |
glucose absorption | 14 |
bca protein | 14 |
cells via | 14 |
wnt agonist | 14 |
derived vectors | 14 |
adapted strain | 14 |
treatment groups | 14 |
mediated isothermal | 14 |
ra plus | 14 |
negative controls | 14 |
infected swine | 14 |
different coronaviruses | 14 |
distilled water | 14 |
oral vaccines | 14 |
induces apoptosis | 14 |
analytical specificity | 14 |
cells showed | 14 |
natural science | 14 |
critical epitopes | 14 |
mhv di | 14 |
transcription factors | 14 |
enteric tract | 14 |
saturable binding | 14 |
middle east | 14 |
days cultivation | 14 |
transferred onto | 14 |
early stage | 14 |
also showed | 14 |
intestinal tissue | 14 |
maintenance medium | 14 |
acid residues | 14 |
least three | 14 |
virus neutralizing | 14 |
porcine small | 14 |
two strains | 14 |
targeting shrna | 14 |
strand cdna | 14 |
nucleocapsid gene | 14 |
piglets infected | 14 |
mammary gland | 14 |
antigenic determinants | 14 |
protective efficacy | 14 |
fold change | 14 |
competition assay | 14 |
vaccinated gilts | 14 |
replicase gene | 14 |
recent years | 14 |
swinecheck tgev | 14 |
gene products | 14 |
papn cells | 14 |
virus particle | 14 |
source data | 14 |
see ref | 14 |
inactivated vaccines | 14 |
immunized piglets | 14 |
antigenic relationships | 14 |
tlr expression | 14 |
vectors expressing | 14 |
target gene | 14 |
active immunity | 14 |
east respiratory | 14 |
virus recovery | 14 |
ts recombinants | 14 |
contact time | 14 |
protective immune | 14 |
cv vero | 14 |
viral determinants | 14 |
cov infection | 13 |
expressing pgpu | 13 |
blocking elisas | 13 |
i i | 13 |
studies showed | 13 |
virulent virus | 13 |
subcellular localization | 13 |
treatment group | 13 |
casei shirota | 13 |
low ph | 13 |
considered statistically | 13 |
prcv rbd | 13 |
oral delivery | 13 |
virus shedding | 13 |
differential diagnosis | 13 |
monkey kidney | 13 |
susceptible cells | 13 |
hx strain | 13 |
oral route | 13 |
protein bands | 13 |
nitric oxide | 13 |
dna fragment | 13 |
attenuated virus | 13 |
attenuated vaccine | 13 |
indicated time | 13 |
virus expressing | 13 |
time quantitative | 13 |
gfp nc | 13 |
virus infections | 13 |
via activation | 13 |
north america | 13 |
nucleocapsid proteins | 13 |
veterinary medicine | 13 |
brush border | 13 |
fever virus | 13 |
orf protein | 13 |
antiviral effects | 13 |
animal health | 13 |
field isolates | 13 |
intestinal mucosa | 13 |
inoculated piglets | 13 |
gibco brl | 13 |
pbs group | 13 |
inhibited tgev | 13 |
plasmid ppg | 13 |
virology doi | 13 |
based vaccines | 13 |
four structural | 13 |
gene deletion | 13 |
ml streptomycin | 13 |
avian coronavirus | 13 |
diarrhea coronavirus | 13 |
egfr internalization | 13 |
ii strain | 13 |
tgev cdna | 13 |
different cell | 13 |
significantly enhanced | 13 |
days postinoculation | 13 |
casei vaccine | 13 |
intestinal tract | 13 |
mucosal surfaces | 13 |
pig model | 13 |
molecular mass | 13 |
swine fever | 13 |
prcv recombinant | 13 |
swine testicular | 13 |
cultured enterocytes | 13 |
vaccine development | 13 |
significantly inhibited | 13 |
targeting shrnas | 13 |
technical assistance | 13 |
mice orally | 13 |
new coronavirus | 13 |
enteric infections | 13 |
viral diarrhea | 13 |
start codon | 13 |
helicase ddx | 13 |
natural host | 13 |
canine coronaviruses | 13 |
large white | 13 |
group recombinants | 13 |
virus may | 13 |
neutralizing activity | 13 |
recombinant swinepox | 13 |
also detected | 13 |
inhibitory activity | 13 |
receptor recognition | 13 |
cellular immune | 13 |
plus tgev | 13 |
type virus | 13 |
start sites | 13 |
among coronaviruses | 13 |
leader sequences | 12 |
conventional rt | 12 |
error bars | 12 |
pcr rt | 12 |
terminal signal | 12 |
farm animals | 12 |
sequence data | 12 |
quantitative pcr | 12 |
extraction kit | 12 |
prrsv gp | 12 |
activated protein | 12 |
fold lower | 12 |
antibody detection | 12 |
respiratory tropism | 12 |
mhv strains | 12 |
transiently transfected | 12 |
specific receptors | 12 |
immunized mice | 12 |
per group | 12 |
kidney cell | 12 |
different virus | 12 |
primed transcription | 12 |
takes place | 12 |
porcine parvovirus | 12 |
pig industry | 12 |
using anti | 12 |
small membrane | 12 |
weight loss | 12 |
plasmids expressing | 12 |
tgev inactivated | 12 |
coronavirus defective | 12 |
attenuated rv | 12 |
ssc low | 12 |
like particle | 12 |
situ hybridization | 12 |
avian influenza | 12 |
using lipofectamine | 12 |
two orfs | 12 |
four groups | 12 |
indicated times | 12 |
tgev may | 12 |
rna species | 12 |
protein domains | 12 |
tritrichomonas foetus | 12 |
nonstructural proteins | 12 |
culture plates | 12 |
ribosomal entry | 12 |
inactivated vaccine | 12 |
piglets born | 12 |
antiviral response | 12 |
high titers | 12 |
cytopathic effects | 12 |
tgev genes | 12 |
primary antibody | 12 |
specific igg | 12 |
labeled antibodies | 12 |
lymphoid tissue | 12 |
using real | 12 |
subunit vaccine | 12 |
post inoculation | 12 |
intestinal homeostasis | 12 |
result showed | 12 |
antigenic cross | 12 |
virus taxonomy | 12 |
using trizol | 12 |
capsid protein | 12 |
viral entry | 12 |
recombinase polymerase | 12 |
competing interests | 12 |
order nidovirales | 12 |
permissive sites | 12 |
elisa method | 12 |
inactivated tgev | 12 |
state university | 12 |
also tested | 12 |
results also | 12 |
cells released | 12 |
tgev proteins | 12 |
per ml | 12 |
tgev mrna | 12 |
mice immunized | 12 |
virus virulence | 12 |
alpha interferon | 12 |
molecular mechanisms | 12 |
mock infected | 12 |
protein encoded | 12 |
polymerase amplification | 12 |
mixed infection | 12 |
control groups | 12 |
co atmosphere | 12 |
receptor tyrosine | 12 |
egfp expression | 12 |
real time | 12 |
scale bar | 12 |
directed mutagenesis | 12 |
specific monoclonal | 12 |
bacterial artificial | 12 |
standard curves | 12 |
actin filaments | 12 |
samples collected | 12 |
rna copies | 12 |
per reaction | 12 |
challenge virus | 12 |
cleavage site | 12 |
qpcr assay | 12 |
entry process | 12 |
intestinal iga | 12 |
four times | 12 |
pcr assays | 12 |
elisa plates | 12 |
tgev pedv | 12 |
crypt cells | 12 |
regulatory factor | 12 |
gene source | 12 |
dna polymerase | 12 |
intestinal villi | 12 |
viral nucleocapsid | 12 |
first described | 12 |
national natural | 12 |
deletion mutant | 12 |
pig herds | 12 |
international committee | 12 |
recombinant proteins | 12 |
presenting cells | 11 |
see section | 11 |
serial dilutions | 11 |
igg fitc | 11 |
south korea | 11 |
mediated signaling | 11 |
th cells | 11 |
gst pulldown | 11 |
pedv orf | 11 |
primers used | 11 |
recombinant dna | 11 |
hydrophobic protein | 11 |
membrane glycoprotein | 11 |
randomly divided | 11 |
structural components | 11 |
coronavirus isolates | 11 |
contains two | 11 |
viral enteritis | 11 |
visualized using | 11 |
cell type | 11 |
high homology | 11 |
animal experiments | 11 |
secretory pathway | 11 |
culture media | 11 |
assay kit | 11 |
recombinant elisa | 11 |
cell tropism | 11 |
three different | 11 |
pathological changes | 11 |
shaanxi strain | 11 |
time reverse | 11 |
results provide | 11 |
sybr green | 11 |
transmembrane glycoprotein | 11 |
different groups | 11 |
tgev infections | 11 |
conventional pcr | 11 |
matrix protein | 11 |
attachment ability | 11 |
obtained using | 11 |
infection causes | 11 |
five times | 11 |
protein interaction | 11 |
initiation factor | 11 |
prcv isolate | 11 |
upper respiratory | 11 |
evolutionary relationship | 11 |
enveloped virus | 11 |
taqman probe | 11 |
ribosomal frameshifting | 11 |
total number | 11 |
mitochondrial ca | 11 |
positive results | 11 |
may explain | 11 |
inducing factor | 11 |
probiotic strains | 11 |
porcine cell | 11 |
cofilin activity | 11 |
porcine hemagglutinating | 11 |
reduction factor | 11 |
lg well | 11 |
immune complexes | 11 |
human cells | 11 |
rna molecules | 11 |
elisa using | 11 |
interferon production | 11 |
commonly used | 11 |
transmembrane domain | 11 |
culture fluid | 11 |
like receptors | 11 |
dna vaccine | 11 |
initiation codon | 11 |
luciferase assay | 11 |
target genes | 11 |
complementation groups | 11 |
glucose transport | 11 |
homology among | 11 |
accession numbers | 11 |
viral infectivity | 11 |
enteric virus | 11 |
feline enteric | 11 |
performed according | 11 |
rhamnosus gg | 11 |
parental virus | 11 |
digestive tract | 11 |
mtt assay | 11 |
cytokine responses | 11 |
encoded proteins | 11 |
genomic sequence | 11 |
virus detection | 11 |
known porcine | 11 |
active site | 11 |
cell supernatants | 11 |
ssc high | 11 |
field isolate | 11 |
neonatal calf | 11 |
confluent monolayers | 11 |
protective effect | 11 |
gal promoter | 11 |
inhibits tgev | 11 |
antigen alone | 11 |
labeled tgev | 11 |
molecular epidemiology | 11 |
using tgev | 11 |
replication cycle | 11 |
thermo fisher | 11 |
lentiviral particles | 11 |
green fluorescent | 11 |
cv fecal | 11 |
mock control | 11 |
serial passages | 11 |
node cells | 11 |
group showed | 11 |
antigen expression | 11 |
forming units | 11 |
muench method | 11 |
new insights | 11 |
high morbidity | 11 |
fusion sites | 11 |
peplomer glycoprotein | 11 |
statistical significance | 11 |
receptor interaction | 11 |
nervous system | 11 |
potential role | 11 |
cfu ml | 11 |
protein fragments | 11 |
treated viruses | 11 |
ig sequences | 11 |
investigated whether | 11 |
oral tgev | 11 |
od values | 11 |
significantly decreased | 11 |
days old | 11 |
sample buffer | 11 |
quantitative proteomic | 11 |
detect tgev | 11 |
egfp empty | 11 |
southern china | 10 |
wt group | 10 |
tgev detection | 10 |
tgev positive | 10 |
potato tubers | 10 |
secretory iga | 10 |
evaluated using | 10 |
dependent pathway | 10 |
study group | 10 |
binding specificity | 10 |
containing fetal | 10 |
virulent us | 10 |
viral spike | 10 |
recently reported | 10 |
leukemia virus | 10 |
sequence identity | 10 |
virus vector | 10 |
orf encodes | 10 |
rbd tip | 10 |
sucking piglets | 10 |
unique restriction | 10 |
results demonstrate | 10 |
significantly increase | 10 |
old pigs | 10 |
specific mab | 10 |
genome expression | 10 |
viruses may | 10 |
breeding farms | 10 |
protein concentrations | 10 |
virus vectors | 10 |
mock group | 10 |
mosaic virus | 10 |
protein using | 10 |
signal transduction | 10 |
delivery systems | 10 |
bars represent | 10 |
ig site | 10 |
accessory proteins | 10 |
enters cells | 10 |
protein result | 10 |
highly virulent | 10 |
administered orally | 10 |
viral structural | 10 |
rabies virus | 10 |
among different | 10 |
online version | 10 |
cellular factors | 10 |
sized rna | 10 |
prcv infection | 10 |
post transfection | 10 |
rtgev viruses | 10 |
binding proteins | 10 |
viral disease | 10 |
detection methods | 10 |
spleen cells | 10 |
nc sirna | 10 |
extracted using | 10 |
diagnostic specificity | 10 |
growth kinetics | 10 |
nucleic acids | 10 |
detection system | 10 |
subviral particles | 10 |
genetic evolution | 10 |
relationships among | 10 |
investigate whether | 10 |
vaccine group | 10 |
feeder pigs | 10 |
prrsv antigens | 10 |
ccid ml | 10 |
papn knockout | 10 |
using two | 10 |
feline coronaviruses | 10 |
viral genes | 10 |
recombination frequency | 10 |
weaning mortality | 10 |
primer sequences | 10 |
pedv rna | 10 |
binding region | 10 |
neutralizing mabs | 10 |
encoded nsp | 10 |
quantitative rt | 10 |
fluorescent protein | 10 |
critical role | 10 |
crystal structures | 10 |
prime boost | 10 |
different tgev | 10 |
spike proteins | 10 |
attachment sites | 10 |
coronavirus membrane | 10 |
adaptive immune | 10 |
local immune | 10 |
receptor determinants | 10 |
pig farm | 10 |
mouse model | 10 |
data indicated | 10 |
laboratory animals | 10 |
goat serum | 10 |
yeast two | 10 |
large amounts | 10 |
previous research | 10 |
different species | 10 |
natural infection | 10 |
genetic markers | 10 |
dii promoter | 10 |
control gilts | 10 |
large rna | 10 |
coronavirus receptor | 10 |
intestinal enterocytes | 10 |
fipv strain | 10 |
swine coronaviruses | 10 |
genome encodes | 10 |
antigen sites | 10 |
swine herd | 10 |
vaccinated sows | 10 |
orally inoculated | 10 |
circovirus type | 10 |
stably expressed | 10 |
purdue virus | 10 |
sequence homology | 10 |
tgev antigens | 10 |
primary structure | 10 |
endonuclease sites | 10 |
mhv receptor | 10 |
plaque assays | 10 |
duplex mmps | 10 |
culture system | 10 |
mabs specific | 10 |
highly pathogenic | 10 |
swab fluids | 10 |
single mmps | 10 |
artificial chromosome | 10 |
host innate | 10 |
selective marker | 10 |
data shown | 10 |
recombinant strains | 10 |
three recombinant | 10 |
length genome | 10 |
binding sequences | 10 |
cellular processes | 10 |
six times | 10 |
veterinary microbiology | 10 |
sars virus | 10 |
dengue virus | 10 |
classical swine | 10 |
standard deviations | 10 |
macrophage cell | 10 |
protective lactogenic | 10 |
surface receptors | 10 |
culture infective | 10 |
blood samples | 10 |
infection may | 10 |
cholera toxin | 10 |
chimeric virus | 10 |
coronavirus genes | 10 |
aif translocation | 10 |
tgev protein | 10 |
antibody titer | 10 |
genital tract | 10 |
five piglets | 10 |
subgenomic rna | 10 |
recombination demonstrates | 10 |
porcine ileum | 10 |
whether mir | 10 |
coronavirus transcription | 10 |
viruses containing | 10 |
foreign gene | 10 |
plants expressing | 10 |
additional receptor | 10 |
synchronized cells | 10 |
key role | 10 |
strain miller | 10 |
milk antibodies | 10 |
copies per | 10 |
deduced amino | 10 |
coronavirus cell | 10 |
infect cells | 10 |
first time | 10 |
pfcrn promoter | 10 |
reverse transcribed | 10 |
coronavirus leader | 10 |
egfr expression | 10 |
rtgev vectors | 10 |
different types | 10 |
tgev binding | 10 |
tgev tmk | 10 |
assay system | 10 |
length glycoprotein | 10 |
nuclear factor | 10 |
tgev antibody | 10 |
cellular mirnas | 10 |
cdna synthesis | 10 |
viral pathogenesis | 10 |
findings suggest | 10 |
hp attenuated | 10 |
dendritic cell | 10 |
small interfering | 10 |
com scientificreports | 10 |
pig igg | 10 |
rv vaccines | 9 |
virion proteins | 9 |
propidium iodide | 9 |
encephalitis virus | 9 |
infective dose | 9 |
antibody titres | 9 |
might play | 9 |
internal ribosomal | 9 |
dna plasmids | 9 |
antibody staining | 9 |
labelled virus | 9 |
rna dna | 9 |
significant increase | 9 |
virus tgev | 9 |
infectious diseases | 9 |
negative strand | 9 |
soluble protein | 9 |
green monkey | 9 |
tissue sections | 9 |
mab specific | 9 |
mapk signaling | 9 |
unpublished results | 9 |
mad clone | 9 |
restriction fragment | 9 |
serum neutralization | 9 |
intestinal glucose | 9 |
prcv antibodies | 9 |
attenuated strain | 9 |
well culture | 9 |
reproductive tract | 9 |
pcr system | 9 |
plantarum pca | 9 |
blot assay | 9 |
agarose beads | 9 |
severe dehydration | 9 |
transfection reagent | 9 |
regulating sequences | 9 |
virus budding | 9 |
single amino | 9 |
splenic lymphocytes | 9 |
pcr detection | 9 |
hyperimmune sera | 9 |
culture collection | 9 |
stress fibers | 9 |
vero cell | 9 |
conventional duplex | 9 |
residues involved | 9 |
expressing cells | 9 |
cdna fragments | 9 |
vaccine production | 9 |
virus antigen | 9 |
essential role | 9 |
antigenic homology | 9 |
viral rnas | 9 |
labeled goat | 9 |
virus cluster | 9 |
akt pathway | 9 |
codon usage | 9 |
worth noting | 9 |
simple method | 9 |
sequence alignment | 9 |
study provides | 9 |
strain isolated | 9 |
protein binding | 9 |
whether ra | 9 |
vaccine strains | 9 |
cells grown | 9 |
infectious disease | 9 |
cell division | 9 |
post seeding | 9 |
tfr expression | 9 |
based tgev | 9 |
viral components | 9 |
low level | 9 |
attenuated vaccines | 9 |
novel swine | 9 |
cell migration | 9 |
virus date | 9 |
cells ml | 9 |
moi tgev | 9 |
cell adhesion | 9 |
complete sequence | 9 |
central nervous | 9 |
free water | 9 |
two weeks | 9 |
spleen lymphocytes | 9 |
containing rna | 9 |
dna fragments | 9 |
economic impact | 9 |
protein interacts | 9 |
nested rt | 9 |
using specific | 9 |
mimics control | 9 |
fluorescein isothiocyanate | 9 |
blotting using | 9 |
terminal residues | 9 |
animals showed | 9 |
duplex pcr | 9 |
selective pressure | 9 |
untranslated region | 9 |
different times | 9 |
sirna targeting | 9 |
dose dependent | 9 |
specific iga | 9 |
genomic dna | 9 |
pathogenic coronavirus | 9 |
terminal amino | 9 |
egg yolk | 9 |
recombinant lactic | 9 |
group i | 9 |
tgev infects | 9 |
rna packaging | 9 |
different strains | 9 |
phosphorylated stat | 9 |
large deletion | 9 |
van nieuwstadt | 9 |
small intestines | 9 |
transcription start | 9 |
high sensitivity | 9 |
coronavirus nonstructural | 9 |
strain pur | 9 |
srcr domain | 9 |
attenuated live | 9 |
endogenous tfr | 9 |
samhi fragment | 9 |
genetic characterization | 9 |
mediated elisa | 9 |
double immunofluorescence | 9 |
data demonstrate | 9 |
tgev infectivity | 9 |
corn vaccine | 9 |
coli heat | 9 |
infection caused | 9 |
airborne virus | 9 |
expressed using | 9 |
decreased tgev | 9 |
way analysis | 9 |
viral glycoprotein | 9 |
reduction factors | 9 |
primers targeting | 9 |
homing receptor | 9 |
host antiviral | 9 |
day post | 9 |
porcine insulin | 9 |
laser scanning | 9 |
cells expressed | 9 |
lymphoid cells | 9 |
rotavirus strains | 9 |
one day | 9 |
targeted rna | 9 |
minimum essential | 9 |
immunized group | 9 |
virus nucleocapsid | 9 |
original us | 9 |
expressing prrsv | 9 |
mitochondrial function | 9 |
apoptosis induced | 9 |
antigens expressed | 9 |
host immune | 9 |
cell receptors | 9 |
sequence requirement | 9 |
virus suspension | 9 |
fisher scientific | 9 |
bf sha | 9 |
reactive oxygen | 9 |
significant changes | 9 |
humoral response | 9 |
low levels | 9 |
assay reagent | 9 |
coronavirus pur | 9 |
human health | 9 |
complete genomic | 9 |
major cause | 9 |
inoculated onto | 9 |
porcine delta | 9 |
serological tests | 9 |
large number | 9 |
tfr ab | 9 |
normal cells | 9 |
stable expression | 9 |
final concentration | 9 |
coronavirus assembly | 9 |
virus yield | 9 |
virulent strains | 9 |
acetylneuraminic acid | 9 |
pl pro | 9 |
homologous recombination | 9 |
infectious pedv | 9 |
tgev seronegative | 9 |
enhanced chemiluminescence | 9 |
oxygen species | 9 |
animal welfare | 9 |
whole cell | 9 |
vaccine efficacy | 9 |
type iii | 9 |
two days | 9 |
cells fixed | 9 |
weight gain | 9 |
inhibit tgev | 9 |
domestic animals | 9 |
selected characteristics | 9 |
immunofluorescence assay | 9 |
high cells | 9 |
animal care | 9 |
enteric alphacoronavirus | 9 |
apn receptor | 9 |
coated pits | 9 |
neonatal fc | 9 |
membrane microdomains | 9 |
following oral | 9 |
mucosal adjuvants | 9 |
standard procedures | 9 |
antigenic domains | 9 |
applied biosystems | 9 |
treated tgev | 9 |
prok i | 9 |
viral titer | 9 |
protein sequences | 9 |
molecular features | 9 |
inhibitory effects | 9 |
containing mm | 9 |
tgev causes | 9 |
neutralization index | 9 |
induced activation | 9 |
common mucosal | 9 |
mucosal vaccines | 9 |
surface protein | 9 |
low cells | 9 |
protein concentration | 9 |
cell lysate | 9 |
animal coronaviruses | 9 |
interspecies transmission | 9 |
mass spectrometry | 9 |
bone marrow | 9 |
experiments using | 9 |
quantitative real | 9 |
prcv blocking | 9 |
containing fbs | 9 |
template rna | 9 |
current study | 9 |
mek erk | 9 |
sequence comparison | 9 |
virulent british | 9 |
length infectious | 9 |
pfu cell | 9 |
unclassified mabs | 9 |
uranyl acetate | 9 |
neutralization titers | 9 |
parental rtgev | 9 |
fold dilutions | 9 |
mhv strain | 9 |
rpa assay | 9 |
higher levels | 9 |
two porcine | 9 |
fitc labeled | 8 |
similar observations | 8 |
acid substitutions | 8 |
table ii | 8 |
jurisdictional claims | 8 |
studies using | 8 |
oral inoculation | 8 |
binding domains | 8 |
five days | 8 |
intestinal segments | 8 |
cytopathogenic virus | 8 |
maize seed | 8 |
like proteinase | 8 |
anpep wt | 8 |
small envelope | 8 |
salmonella typhimurium | 8 |
based duplex | 8 |
coronaviruses date | 8 |
phase arrest | 8 |
newcastle disease | 8 |
constructed using | 8 |
two doses | 8 |
sucrose gradient | 8 |
unpublished data | 8 |
viral life | 8 |
tb wcl | 8 |
binding assay | 8 |
intracellular transport | 8 |
radiolabelled virus | 8 |
variant strains | 8 |
glycosylation sites | 8 |
per animal | 8 |
may affect | 8 |
solution containing | 8 |
firefly luciferase | 8 |
vaccine candidates | 8 |
dry milk | 8 |
shrnas targeting | 8 |
reduced tgev | 8 |
morphological changes | 8 |
expressed mirnas | 8 |
domain complexed | 8 |
hamster kidney | 8 |
related cov | 8 |
significant economic | 8 |
cov replication | 8 |
fecal suspensions | 8 |
long noncoding | 8 |
protein complex | 8 |
neutralizing epitope | 8 |
described elsewhere | 8 |
protein sequence | 8 |
conformational changes | 8 |
ii cd | 8 |
coronavirus oc | 8 |
industry worldwide | 8 |
neonatal gnotobiotic | 8 |
lentiviruses pseudotyped | 8 |
ch gd | 8 |
gene cassette | 8 |
plaque formation | 8 |
rectal swab | 8 |
dna viruses | 8 |
labile enterotoxin | 8 |
supplementary file | 8 |
virus genes | 8 |
type culture | 8 |
electron microscopic | 8 |
studies demonstrated | 8 |
smooth muscle | 8 |
induce mucosal | 8 |
restriction enzyme | 8 |
naturally infected | 8 |
proteins involved | 8 |
analysis revealed | 8 |
first inoculation | 8 |
jnk phosphorylation | 8 |
lung tissue | 8 |
complete protection | 8 |
mink sera | 8 |
two cell | 8 |
signaling technology | 8 |
nasal shedding | 8 |
infectious dose | 8 |
cell extracts | 8 |
including tgev | 8 |
total soluble | 8 |
lung damage | 8 |
apoptotic rate | 8 |
animal species | 8 |
pedv entry | 8 |
first step | 8 |
particle associated | 8 |
minimal essential | 8 |
serologic cross | 8 |
higher infection | 8 |
act meth | 8 |
purified using | 8 |
od value | 8 |
genetic analysis | 8 |
surface expression | 8 |
antigens induced | 8 |
reduced clinical | 8 |
virol doi | 8 |
remains neutral | 8 |
irrelevant sirna | 8 |
knockdown cells | 8 |
severe clinical | 8 |
nuclear transfer | 8 |
rpd cells | 8 |
african green | 8 |
genus deltacoronavirus | 8 |
promotes transmissible | 8 |
low cell | 8 |
structural domains | 8 |
attenuated salmonella | 8 |
interfering rnas | 8 |
springer nature | 8 |
endocytic pathway | 8 |
faecium ncimb | 8 |
infected control | 8 |
within orf | 8 |
large surface | 8 |
cellular vimentin | 8 |
cov rt | 8 |
rna genomes | 8 |
tgev genomic | 8 |
lymphocyte proliferation | 8 |
new virus | 8 |
alkaline phosphatase | 8 |
vivo virus | 8 |
may result | 8 |
three weeks | 8 |
coronavirus induces | 8 |
high glucose | 8 |
binding edge | 8 |
antibodies induced | 8 |
two tgev | 8 |
antibody production | 8 |
polyvinylidene difluoride | 8 |
young pigs | 8 |
high frequency | 8 |
ml trypsin | 8 |
mdbk cells | 8 |
variant porcine | 8 |
nidovirales order | 8 |
mononuclear cells | 8 |
prcv differential | 8 |
conditioned medium | 8 |
received ml | 8 |
challenged animals | 8 |
expression data | 8 |
dko group | 8 |
secondary structures | 8 |
serologically related | 8 |
economically important | 8 |
five isolates | 8 |
pgk promoter | 8 |
virus expression | 8 |
creative commons | 8 |
ns proteins | 8 |
associated protein | 8 |
tmk strain | 8 |
converting enzyme | 8 |
apoptotic pathway | 8 |
wt pams | 8 |
recombinants ad | 8 |
persistent infection | 8 |
der zeijst | 8 |
experimental pigs | 8 |
crucial role | 8 |
leader rnas | 8 |
well tissue | 8 |
equal volume | 8 |
sg mutant | 8 |
targeting rb | 8 |
ucuaa repeats | 8 |
loop structure | 8 |
underlying mechanism | 8 |
also known | 8 |
weeks later | 8 |
ribosomal frame | 8 |
day old | 8 |
premix ex | 8 |
available data | 8 |
glycolylneuraminic acid | 8 |
field samples | 8 |
cells transformed | 8 |
higher expression | 8 |
speed centrifugation | 8 |
hours post | 8 |
confocal laser | 8 |
reduction assay | 8 |
although tgev | 8 |
aa residues | 8 |
orf mabs | 8 |
human aminopeptidase | 8 |
tcid animal | 8 |
coding sequence | 8 |
following incubation | 8 |
cell nuclei | 8 |
antibodies containing | 8 |
consensus sequences | 8 |
mediated immune | 8 |
pcr methods | 8 |
recombinants expressing | 8 |
infectious tgev | 8 |
intestinal mucosal | 8 |
might also | 8 |
promotes tgev | 8 |
fatal diarrhea | 8 |
commercial blocking | 8 |
compatible ends | 8 |
recent study | 8 |
rotavirus vp | 8 |
sequence requirements | 8 |
functional analysis | 8 |
clinical specimens | 8 |
genes encoding | 8 |
immunoglobulin classes | 8 |
protein binds | 8 |
prcv seropositive | 8 |
stably transformed | 8 |
lactobacillus acidophilus | 8 |
nucleotide sequences | 8 |
sglt expression | 8 |
supplementary material | 8 |
sp antigen | 8 |
genetic stability | 8 |
competition elisa | 8 |
competitive binding | 8 |
better understanding | 8 |
fecal swabs | 8 |
norwalk virus | 8 |
arch virol | 8 |
ig sites | 8 |
host proteins | 8 |
zinc ion | 8 |
test whether | 8 |
second receptor | 8 |
infection group | 8 |
tgev induced | 8 |
three piglets | 8 |
positive serum | 8 |
new england | 8 |
reporter plasmids | 8 |
neutralization determinants | 8 |
gene product | 8 |
virus isolates | 8 |
many viruses | 8 |
copy numbers | 8 |
tropism determinant | 8 |
using serum | 8 |
causes severe | 8 |
several studies | 8 |
fixative preparation | 8 |
antibody test | 8 |
infection resulted | 8 |
iga levels | 8 |
also demonstrated | 8 |
i virus | 8 |
institutional affiliations | 8 |
guinea pigs | 8 |
papn protein | 8 |
tgev grna | 8 |
commercial tgev | 8 |
functional domains | 8 |
nitrocellulose membranes | 8 |
effective vaccines | 8 |
microbiol doi | 8 |
cd knockout | 8 |
per cell | 8 |
strain cv | 8 |
nucleotide differences | 8 |
blank control | 8 |
using purified | 8 |
without fbs | 8 |
control pigs | 8 |
gene silencing | 8 |
indicated primary | 8 |
xhoi site | 8 |
expression via | 8 |
pigs porcine | 8 |
extracellular region | 8 |
scanning electron | 8 |
culture infectious | 8 |
incubated overnight | 8 |
luciferase activities | 8 |
virion envelope | 8 |
influenza vaccine | 8 |
pig production | 8 |
rna extracted | 8 |
four hours | 8 |
previous work | 8 |
polyacrylamide gels | 8 |
virus common | 8 |
animals infected | 8 |
field conditions | 8 |
vaccinated pigs | 8 |
two piglets | 8 |
newborn piglet | 8 |
oral lactobacillus | 8 |
virus capsid | 8 |
structural polypeptides | 8 |
uv light | 8 |
deficient virus | 8 |
tgev structural | 8 |
published maps | 8 |
human intestinal | 8 |
intestinal crypt | 8 |
fluorescent probe | 8 |
old piglet | 8 |
nature remains | 8 |
five tgev | 8 |
multiplex pcr | 8 |
transmembrane protein | 8 |
tgev polyclonal | 8 |
available vaccines | 8 |
enteric diseases | 8 |
european countries | 8 |
humoral immunity | 7 |
samples using | 7 |
older pigs | 7 |
virus challenge | 7 |
tract infections | 7 |
stained cells | 7 |
substrate solution | 7 |
infected tissue | 7 |
elisa test | 7 |
differential detection | 7 |
immunoelectron microscopy | 7 |
iscs fate | 7 |
coronavirus attachment | 7 |
oronasally inoculated | 7 |
one piglet | 7 |
putative second | 7 |
insect cells | 7 |
altered mirnas | 7 |
two distinct | 7 |
ha antibody | 7 |
porcine viruses | 7 |
control serum | 7 |
hybrid system | 7 |
lncrna tcons | 7 |
proteolytic processing | 7 |
mhv infection | 7 |
inflammatory responses | 7 |
spei site | 7 |
alphacoronavirus genus | 7 |
european prcv | 7 |
conjugated anti | 7 |
relative gene | 7 |
pregnant gilts | 7 |
coronavirus species | 7 |
two times | 7 |
syncytium formation | 7 |
microplate reader | 7 |
nuclear extracts | 7 |
specific siga | 7 |
antiviral innate | 7 |
infectious bursal | 7 |
strain wh | 7 |
another study | 7 |
bovine rotavirus | 7 |
size reduction | 7 |
based elisa | 7 |
rotavirus diarrhea | 7 |
stop codon | 7 |
miller strains | 7 |
vectors based | 7 |
kappab activation | 7 |
specific probes | 7 |
lactobacillus rhamnosus | 7 |
bat coronaviruses | 7 |
results support | 7 |
epitopes recognized | 7 |
spleen tissue | 7 |
mhv rna | 7 |
may represent | 7 |
antibodies specific | 7 |
specific neutralizing | 7 |
virion rna | 7 |
structural bases | 7 |
genus alphacoronavirus | 7 |
mm dtt | 7 |
neutralising antibodies | 7 |
unlike tgev | 7 |
gene fragments | 7 |
using recombinant | 7 |
fusion activity | 7 |
transcription loop | 7 |
virus titration | 7 |
units ml | 7 |
baby pigs | 7 |
mrna fusion | 7 |
intestinal columnar | 7 |
based multiplex | 7 |
specific pathogen | 7 |
value obtained | 7 |
tgev coronavirus | 7 |
mean values | 7 |
based oral | 7 |
medium supplemented | 7 |
cells increased | 7 |
swine population | 7 |
coronavirus differs | 7 |
adhesion molecule | 7 |
may interact | 7 |
phylogenetic tree | 7 |
strain grown | 7 |
frame shift | 7 |
decreased significantly | 7 |
following infection | 7 |
enteric diarrhea | 7 |
also evaluated | 7 |
polyclonal anti | 7 |
experimental data | 7 |
conserved regions | 7 |
using mabs | 7 |
display library | 7 |
orf sequence | 7 |
expression plasmids | 7 |
diseased pigs | 7 |
minute contact | 7 |
culture immunofluorescence | 7 |
dna sequence | 7 |
rna levels | 7 |
ros release | 7 |
plaque forming | 7 |
tgev epitopes | 7 |
two virus | 7 |
turkey coronavirus | 7 |
bacillus subtilis | 7 |
cycle threshold | 7 |
serological evidence | 7 |
using either | 7 |
alexa fluor | 7 |
nsp protein | 7 |
protein connect | 7 |
polypeptide structure | 7 |
repeated three | 7 |
fold differences | 7 |
viral invasion | 7 |
tgev recombinants | 7 |
microscopic lesions | 7 |
ng per | 7 |
sendai virus | 7 |
british isolate | 7 |
tagged pedv | 7 |
red fluorescence | 7 |
vaccination strategies | 7 |
tgev virus | 7 |
four genera | 7 |
induces protective | 7 |
pcr primers | 7 |
barrier function | 7 |
coronavirus jhm | 7 |
response elicited | 7 |
chemically synthesized | 7 |
higher neutralizing | 7 |
intestinal tissues | 7 |
uninoculated sows | 7 |
direct evidence | 7 |
systematic assembly | 7 |
confluent st | 7 |
expression profiles | 7 |
results revealed | 7 |
mucosal tissues | 7 |
tge viral | 7 |
high mannose | 7 |
virus transmissible | 7 |
experimental conditions | 7 |
infection activates | 7 |
kbp samhi | 7 |
vaccine antigens | 7 |
binding residues | 7 |
res doi | 7 |
neutralization assay | 7 |
reference strains | 7 |
neonatal mortality | 7 |
ig sequence | 7 |
related viruses | 7 |
host aminopeptidase | 7 |
seronegative sows | 7 |
gnotobiotic piglets | 7 |
nebulizer suspension | 7 |
contagious enteric | 7 |
i ifn | 7 |
molecular interactions | 7 |
thermofisher scientific | 7 |
infected tgev | 7 |
master mix | 7 |
complex binding | 7 |
sulfate polyacrylamide | 7 |
protein band | 7 |
since tgev | 7 |
six days | 7 |
supplemental information | 7 |
respiratory variant | 7 |
us strains | 7 |
sterile pbs | 7 |
mutational analysis | 7 |
late stage | 7 |
body weights | 7 |
gst pull | 7 |
mediated pathways | 7 |
surface receptor | 7 |
catalytic triad | 7 |
saccharomyces cerevisiae | 7 |
vaccine delivery | 7 |
virus structural | 7 |
amersham international | 7 |
data showed | 7 |
acid identity | 7 |
enterocyte fractions | 7 |
target sizes | 7 |
particles produced | 7 |
recombinant porcine | 7 |
based methods | 7 |
monitored daily | 7 |
tgev enteric | 7 |
immunofluorescence assays | 7 |
immune cells | 7 |
th responses | 7 |
wasting disease | 7 |
experimental groups | 7 |
ns protein | 7 |
severe economic | 7 |
infection efficiency | 7 |
reaction system | 7 |
test sample | 7 |
damage via | 7 |
protein translation | 7 |
tyrosine kinases | 7 |
entry mediated | 7 |
inhibitor cocktail | 7 |
cruz biotechnology | 7 |
tgev replicates | 7 |
target cell | 7 |
th cell | 7 |
separated proteins | 7 |
viral load | 7 |
genome rna | 7 |
domain iv | 7 |
cell fixation | 7 |
days later | 7 |
inductive site | 7 |
local immunity | 7 |
clinical cases | 7 |
capped transcripts | 7 |
england biolabs | 7 |
lactobacillus strain | 7 |
cell suspensions | 7 |
baby hamster | 7 |
cytokine expression | 7 |
vp protein | 7 |
golgi stack | 7 |
stem cell | 7 |
virus causes | 7 |
using western | 7 |
recombinant antigen | 7 |
porcine alveolar | 7 |
loading buffer | 7 |
luciferase report | 7 |
growth curve | 7 |
mediated immunity | 7 |
transcription recombinase | 7 |
adult pigs | 7 |
fcrn expression | 7 |
cycle profiles | 7 |
coronaviruses related | 7 |
fusion proteins | 7 |
free leader | 7 |
tgev seropositive | 7 |
protease activity | 7 |
growth factors | 7 |
us porcine | 7 |
reporter gene | 7 |
recombinant baculovirus | 7 |
mab binding | 7 |
outer membrane | 7 |
virus isolated | 7 |
binding assays | 7 |
plant extracts | 7 |
live tgev | 7 |
strain jhm | 7 |
virus enters | 7 |
laser confocal | 7 |
oral fluid | 7 |
tgev co | 7 |
proteolytic cleavage | 7 |
influenza viruses | 7 |
coronavirus subgenomic | 7 |
diarrheal disease | 7 |
corona virus | 7 |
syncytia formation | 7 |
gene using | 7 |
many cell | 7 |
litter survival | 7 |
tgev infectious | 7 |
nursing piglets | 7 |
three structural | 7 |
tgev concentrations | 7 |
reproductive failure | 7 |
aerosolization efficiency | 7 |
cas system | 7 |
veterinary diagnostic | 7 |
correlated positively | 7 |
plasmid containing | 7 |
coronavirus mrna | 7 |
young piglets | 7 |
pig serum | 7 |
sows inoculated | 7 |
rna transcripts | 7 |
early studies | 7 |
attenuated rtgev | 7 |
flanking sequences | 7 |
way anova | 7 |
antibody technique | 7 |
genomic mrnas | 7 |
signal transducer | 7 |
cells without | 7 |
iscs self | 7 |
protein might | 7 |
mesenchymal cells | 7 |
reticulum stress | 7 |
expressed tgev | 7 |
antibodies directed | 7 |
independent manner | 7 |
every days | 7 |
correct orientation | 7 |
primary ileum | 7 |
conformational change | 7 |
cultured without | 7 |
new porcine | 7 |
promote tgev | 7 |
subsequently infected | 7 |
plaque reduction | 7 |
virus growth | 7 |
dewen title | 7 |
infection control | 7 |
pvdf membrane | 7 |
experimentally inoculated | 7 |
mucosal iga | 7 |
plaque purified | 7 |
ex taq | 7 |
respiratory diseases | 7 |
cellular entry | 7 |
jhm strain | 7 |
wide range | 7 |
leader complex | 7 |
also obtained | 7 |
showed similar | 7 |
interferon regulatory | 7 |
competent cells | 7 |
bursal disease | 7 |
sybr premix | 7 |
inoculated sows | 7 |
genetics system | 7 |
pig mortality | 7 |
subgenomic rnas | 7 |
coated onto | 7 |
time point | 7 |
human adenovirus | 7 |
second orf | 7 |
point mutation | 7 |
group remained | 7 |
commercial elisas | 7 |
many years | 7 |
various tgev | 7 |
translation initiation | 7 |
evidence suggests | 7 |
stranded dna | 7 |
phosphorylation level | 7 |
relative expression | 7 |
agricultural university | 7 |
probe dylight | 7 |
columnar epithelial | 7 |
tgev alone | 7 |
downstream signaling | 7 |
egfr synergistically | 7 |
stool samples | 7 |
acting signals | 7 |
envelope glycoproteins | 7 |
rv inoculated | 7 |
confer protection | 7 |
kinase activity | 7 |
may help | 7 |
novel lncrnas | 7 |
veterinary vaccines | 7 |
rbd residues | 7 |
mouse brain | 7 |
also inhibited | 7 |
amplification assay | 7 |
minor structural | 7 |
coronavirus genomes | 7 |
farm animal | 7 |
conjugated mouse | 7 |
muscle actin | 7 |
previous report | 7 |
cause diarrhea | 7 |
sindbis virus | 7 |
cell monolayer | 7 |
different stages | 7 |
tgev tropism | 7 |
clear whether | 7 |
skim milk | 7 |
length spike | 7 |
intramuscular injection | 7 |
respiratory infection | 7 |
estimating fifty | 7 |
may contribute | 7 |
virus glycoprotein | 7 |
cells loss | 7 |
ma cells | 7 |
airborne viruses | 7 |
cdca expression | 7 |
cell debris | 7 |
neutralize tgev | 7 |
partially purified | 7 |
laboratory manual | 7 |
largely unknown | 7 |
american type | 7 |
cells cultured | 7 |
relatively high | 7 |
samples containing | 7 |
large quantities | 6 |
gene stability | 6 |
coronavirus neutralization | 6 |
experimental animals | 6 |
engineered virus | 6 |
infectious bacterial | 6 |
antigenic variation | 6 |
data show | 6 |
heilongjiang province | 6 |
receptor regulates | 6 |
equal amounts | 6 |
following virus | 6 |
determinants essential | 6 |
kdel receptor | 6 |
murine leukemia | 6 |
known coronaviruses | 6 |
prcv detection | 6 |
cd cell | 6 |
orf ab | 6 |
major histocompatibility | 6 |
endocytosis pathway | 6 |
pathogens causing | 6 |
diagnostic laboratory | 6 |
neutralization test | 6 |
side chains | 6 |
times higher | 6 |