trigram

This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

trigram frequency
porcine epidemic diarrhea2712
epidemic diarrhea virus2217
of porcine epidemic1231
in this study447
porcine epidemic diarrhoea385
the presence of342
the united states327
diarrhea virus in324
transmissible gastroenteritis virus319
the s protein300
epidemic diarrhoea virus279
of the s267
the cells were265
in the united259
based on the259
of the virus235
of the pedv232
in vero cells230
according to the217
as well as210
of pedv in195
for min at190
vero cells were188
the s gene188
analysis of the186
infected with pedv180
in the s178
as shown in166
the detection of166
of the porcine165
for h at162
the porcine epidemic160
acute respiratory syndrome158
severe acute respiratory157
respiratory syndrome coronavirus150
in the presence149
for the detection147
the expression of146
was used to145
diarrhea virus infection141
due to the140
pedv n protein140
vero e cells139
the number of138
the present study137
to determine the136
porcine reproductive and135
reproductive and respiratory134
jung et al134
and respiratory syndrome133
pedv s protein129
as described previously129
diarrhea virus strains126
respiratory syndrome virus124
genome sequence of124
in cell culture121
at room temperature121
epidemic diarrhea viruses120
the role of119
the small intestine119
virus in the118
phylogenetic analysis of117
epidemic diarrhea in116
showed that the114
cells in the114
complete genome sequence113
data not shown112
results showed that111
observed in the111
in suckling piglets110
was used as109
one of the108
in order to106
a total of104
the pedv s104
a and b104
in the us104
diarrhea virus strain104
of pedv infection103
was added to103
detected in the103
in the present102
and characterization of101
were used to101
detection of porcine100
the development of99
compared to the99
shown in fig98
was detected in98
sequence analysis of96
sequences of the95
of transmissible gastroenteritis94
detection of pedv94
the n protein94
samples were collected93
were infected with91
were collected from89
pedv and tgev89
chen et al88
the end of88
associated with the88
type i interferon87
the results of87
indicated that the87
of pedv strains87
the spike protein87
as previously described87
diarrhea virus and86
compared with the85
the spike gene85
were incubated with84
used as a84
with diarrhea in83
as described above83
and phylogenetic analysis83
emergence of porcine83
it has been83
virus and porcine83
in the small82
porcine respiratory coronavirus81
of pedv and81
of the spike81
characterization of porcine80
wang et al80
diarrhea in swine80
type i ifn80
we found that80
was performed using79
the effect of79
s and s79
were observed in79
pedv in the79
strain of porcine78
cells were washed78
pedv infection in77
protein of pedv76
were inoculated with76
the n gene76
to that of75
with porcine epidemic75
associated with diarrhea74
this study was74
for h and74
the orf gene74
and porcine epidemic74
three times with74
porcine aminopeptidase n73
was observed in73
rna was extracted73
in the spike73
were treated with72
intestinal epithelial cells72
in the same72
shown in figure72
c for h71
of the viral71
than that of71
of molecular epidemiology71
in the field70
in the usa70
an important role70
characterization of the70
cells were infected70
a new coronavirus69
amino acid sequences69
the results showed68
cells were fixed68
strains in the68
li et al68
analysis of porcine68
us porcine epidemic68
the absence of68
tgev and pedv68
s protein of67
end of the67
of this study67
positive for pedv67
related to the67
cells were incubated66
gastroenteritis virus and66
has been reported66
of a novel66
to investigate the66
isolation and characterization66
washed three times66
was used for65
the authors declare65
in our study65
mouse hepatitis virus65
sequence of a65
in neonatal piglets65
in the pedv65
the replication of64
were detected in64
used in this64
the use of64
for porcine epidemic64
particle associated with63
important role in63
infected vero cells63
review of molecular63
were subjected to63
sequence of the63
of type i63
innate immune response63
strain pc a63
of pedv was63
vero cells and63
infected cells were63
expression of the62
hek t cells62
cells infected with62
and stored at62
in south korea62
the swine industry62
g and g62
a comprehensive review62
comprehensive review of62
there was no62
at h post62
deletion in the62
and viral genomic61
of the small61
gene of porcine61
involved in the61
viral genomic sequences61
found to be61
that of the61
the addition of61
of the disease61
song et al61
prrsv and pedv60
the amino acid60
like particle associated60
to evaluate the60
in the infected60
were positive for60
clinical signs of59
amino acid sequence59
were obtained from59
fetal bovine serum59
against porcine epidemic58
thermo fisher scientific58
of pedv rna58
the activation of58
of viral rna58
in the intestinal58
were transfected with58
for min and57
the nucleocapsid protein57
results indicated that57
the complete genome57
the amount of57
the percentage of57
none of the57
mesenteric lymph nodes56
experimental infection of56
the family coronaviridae56
demonstrated that the56
new variants of56
of icpc a56
closely related to56
found in the56
in addition to56
aminopeptidase n is56
were used as56
virus in china55
variants of porcine55
strain of pedv55
significantly higher than55
genes of porcine55
strains of pedv55
cells were transfected55
by porcine epidemic55
were collected at55
a member of55
the production of55
domain of the55
incubated for h55
on the other55
of vero cells55
transmissible gastroenteritis coronavirus54
development of a54
used for the54
of pedv n54
of the n54
the s and54
of the study54
and in the54
the effects of54
the levels of54
revealed that the53
s gene of53
g for min53
added to the53
with pedv at53
has been shown53
the other hand53
cells were then53
characterization of a53
in the cytoplasm52
was carried out52
in response to52
s protein is52
to pedv infection52
been shown to52
were purchased from52
were stained with52
present in the52
virus spike protein52
was found to52
tbk and irf52
linked immunosorbent assay52
polymerase chain reaction52
coronavirus spike protein52
ma et al51
activation of the51
attenuated porcine epidemic51
presence of pedv51
diarrhea virus spike51
role in the51
the cell surface51
of the orf51
the samples were50
in vitro and50
pedv pl pro50
cells were seeded50
the vero cells50
the positive control50
western blot analysis50
cells were treated50
have been reported50
o u r49
a l p49
lin et al49
genbank accession no49
region of the49
outbreak of porcine49
n a l49
j o u49
were analyzed by49
located in the49
member of the49
u r n49
used in the49
of n protein49
antiviral activity of49
r n a49
was confirmed by49
inoculated with the49
were fixed with48
and incubated with48
p r e48
mutations in the48
to those of48
for the pedv48
cells treated with48
gene of pedv48
were determined by48
in gnotobiotic pigs48
the infectivity of48
l p r48
o o f48
r o o48
was determined by48
p r o48
the surface of47
with the pedv47
was supported by47
in vero e47
the sensitivity of47
in the absence47
was extracted from47
losses to the47
the current study47
s indel pedv47
of a porcine47
obtained from the47
porcine circovirus type47
with ml of47
for detection of47
in suckling pigs47
similar to those47
have been identified46
in the n46
the level of46
care and use46
pedv infection and46
similar to that46
the virulence of46
were incubated for46
the new ica46
s protein was46
evolution of pedv46
pigs of all46
kim et al46
of neutralizing antibodies46
all of the46
during pedv infection46
were carried out46
with pbs and46
jejunum and ileum46
total rna was45
added to each45
of the genome45
viruses isolated in45
the pathogenesis of45
shown in table45
were cultured in45
molecular characterization and45
stevenson et al45
the virus was45
a role in45
the negative control45
of clinical signs45
against pedv infection45
three independent experiments45
an outbreak of45
similar to the44
of all ages44
negative for pedv44
the pedv n44
a variety of44
of the two44
of severe acute44
spike gene of44
to assess the44
pedv rna in44
is characterized by44
virus strains in44
clinical signs and44
diarrhea virus from44
by western blot43
and vero cells43
belongs to the43
showed that pedv43
times with pbs43
human coronavirus e43
that the pedv43
n is a43
blfp crude extract43
pedv strains were43
saif et al43
were performed using43
on porcine epidemic43
is the first43
sensitivity and specificity43
and incubated at43
and transmissible gastroenteritis43
on the surface43
results suggest that43
classical attenuated vaccine43
identified in the43
well as the42
multiplicity of infection42
there were no42
results of the42
in the orf42
the supernatant was42
of pedv s42
propagation of the42
antibodies against pedv42
expression levels of42
authors declare that42
between the two42
c for min42
variant porcine epidemic42
the interaction between42
receptor for the42
the lack of42
and incubated for41
j cells were41
pc a in41
colostrum and milk41
the fact that41
is essential for41
diarrhea in suckling41
high mortality in41
neutralizing antibodies against41
and genotyping of41
type i and41
were washed with41
protein is a41
at the end41
orally inoculated with41
with or without41
induced by ifn41
a number of41
viruses associated with41
of a new41
most of the41
hofmann and wyler41
collected from the41
t cells were41
the s domain41
the pedv strains41
results indicate that41
african green monkey41
the pedv genome40
were found to40
identification of the40
those of the40
genotyping of emergent40
a porcine epidemic40
open reading frame40
emergent porcine epidemic40
consistent with the40
of pedv antigen40
serum samples were40
to determine whether40
host innate immune40
antibodies against the40
of emergent porcine40
huang et al40
that pedv infection40
can be used40
pathogenesis and immunoprophylaxis40
porcine transmissible gastroenteritis40
have shown that40
for the first40
analysis was performed40
green monkey kidney40
infectious bronchitis virus40
in contrast to40
antibodies against porcine39
the duration of39
nucleocapsid protein of39
with a large39
conjugated goat anti39
east respiratory syndrome39
used as the39
to porcine epidemic39
n protein was39
characterization and phylogenetic39
infection of pigs39
been reported in39
the antiviral activity39
cells were grown39
at the same39
found that the39
igg and iga39
were washed three39
attenuated vaccine strains39
was obtained from39
in accordance with39
diarrhea viruses associated39
responsible for the39
of antibodies against39
for pedv and39
were added to39
pedv spike protein39
suggest that the39
of pedv is38
differences in the38
of porcine reproductive38
epidemic diarrhea coronavirus38
is associated with38
specificity of the38
shown to be38
icpc a and38
pedv strain cv38
pcr products were38
were incubated at38
economic losses to38
animal care and38
sensitivity of the38
on the cell38
zhang et al38
used to detect38
the plates were37
swine enteric coronavirus37
of s protein37
with fetal bovine37
morbidity and mortality37
be due to37
the genus alphacoronavirus37
park et al37
min at room37
piglets born to37
strains of porcine37
dried porcine plasma37
with the disease37
virus strain pc37
virus of porcine37
the emergence of37
by centrifugation at37
by western blotting37
of us porcine37
vero and ipec37
to each well37
a previous study37
this work was37
genome sequences of37
the m protein37
the virus of37
of tgev and37
complex evolution of37
and complex evolution37
and western blotting37
a new porcine37
isolated in korea37
but not in37
molecular epidemiology of37
the disease outbreak37
were significantly higher37
distinct characteristics and36
cov n proteins36
middle east respiratory36
the gene source36
as the gene36
the concentration of36
were negative for36
gene of the36
protein of the36
indicating that the36
the first time36
emerging and re36
cd cd t36
cloned into the36
song and park36
of porcine deltacoronavirus36
feline infectious peritonitis36
has not been36
positive ec cells36
cells were cultured36
a functional receptor36
characteristics and complex36
and ch jx36
diarrhea in cell36
inoculated orally with36
is involved in36
s gene sequences36
for the presence36
conflict of interest36
molecular characterization of36
swine in the36
of the new36
gene source of36
approved by the36
the pedv spike36
a negative control36
and subjected to35
disease outbreak among35
and icpc a35
in newborn piglets35
kindly provided by35
listed in table35
rapid detection of35
changes in the35
outbreak among swine35
infected with porcine35
based on a35
to identify the35
pigs in the35
belong to the35
such as the35
to be a35
stage of infection35
as a result35
play a role35
viral rna was35
the cell culture35
type i ifns35
among swine in35
pigs inoculated with35
in the gut35
the ability of35
that they have34
is an enveloped34
the pedv strain34
when compared to34
cell lysates were34
the recombinant s34
the inhibition of34
partial s gene34
well tissue culture34
functional receptor for34
than in the34
study was to34
virus infection in34
are shown in34
of the piglets34
coronaviruses as the34
protein genes of34
of pigs with34
inoculated with pedv34
recombinant s protein34
is required for34
deletions in the34
data indicate that34
the order nidovirales34
were used for34
innate immune responses34
the innate immune34
and n genes34
and use committee34
the phylogenetic tree34
the viral genome34
the s region34
they have no34
spike protein of34
cells were harvested34
intracellular extracts of34
pedv rna was34
were seeded in34
e cells were34
understanding of the34
and avian coronaviruses34
the formation of34
suggesting that the33
were expressed as33
the intracellular extracts33
and de bouck33
infected suckling pigs33
were similar to33
isolation of porcine33
to detect the33
were grown in33
indicate that the33
the pig industry33
adapted porcine epidemic33
for the development33
previous studies have33
were found in33
complete genome sequences33
infected weaned pigs33
in the study33
first reported in33
pigs infected with33
the small intestines33
cells were collected33
in our laboratory33
and sequence analysis33
the spread of33
porcine intestinal epithelial33
economic losses in33
diarrhea viruses isolated33
pensaert and de33
of the coronavirus32
fecal samples were32
sialic acid binding32
small intestine of32
was purchased from32
days of age32
inducing neutralizing antibodies32
then infected with32
depending on the32
were collected and32
the majority of32
the efficacy of32
piglets infected with32
declare that they32
plays an important32
was significantly higher32
pcd and vero32
pedv field isolates32
are expressed as32
infection in the32
balb c mice32
be divided into32
of a variant32
pcr for the32
losses in the32
porcine enteric coronavirus32
with a new32
contribute to the32
the detection limit32
higher than that32
on the s32
well plates were32
feed as a32
plates were incubated32
variant of porcine32
supplemented with fetal32
in a dose32
causative agent of32
with the s32
these results indicated32
cells were inoculated32
different from the32
institutional animal care32
a large deletion32
new variant of32
is the most31
to test the31
to detect pedv31
there is no31
nucleotide sequences of31
is critical for31
vero cells in31
described in the31
s proteins of31
occurred in the31
vero cells with31
lee et al31
of the nucleocapsid31
studies have shown31
high levels of31
detection of antibodies31
capable of inducing31
no significant differences31
and the united31
severe diarrhea and31
piglets in the31
the orf a31
a in gnotobiotic31
an emerging and31
the host cell31
resulted in a31
bovine serum albumin31
for pedv infection31
at hpi and31
fixed with paraformaldehyde31
in the netherlands31
be used to31
the nucleotide sequence31
expressed as the31
that the virus31
new porcine enteric31
as a negative31
the causative agent31
after pedv infection31
it is possible31
and quantification of31
this is the31
epizootic swine virus31
washed with pbs31
a concentration of31
in cell cultures30
receptor for pedv30
the result of30
orf a and30
and amino acid30
large deletion in30
the g b30
caused by pedv30
the s proteins30
and high mortality30
deduced amino acid30
the virus in30
viral rna in30
swine enteric coronaviruses30
differences between the30
the function of30
woo et al30
negative control group30
to confirm the30
pathology of us30
like strain of30
pig farms in30
the time of30
at different time30
the basis of30
is a highly30
the induction of30
in pigs of30
to the swine30
gallien et al30
in the vero30
these results indicate30
in the pig30
been reported to30
replication of pedv30
were maintained in30
and mortality in30
on the basis30
in the intestine30
of pedv were30
pigs with a30
final concentration of30
h and then30
n protein is30
the prevalence of30
levels in the30
with the same29
work was supported29
of the pigs29
is responsible for29
pedv belgorod dom29
of the complete29
jung and saif29
samples collected from29
presence of trypsin29
the severe acute29
detection and quantification29
any of the29
a variant porcine29
the partial s29
resulting in the29
acid sequences of29
in the current29
min at rt29
suggested that the29
was first reported29
this study were29
samples were tested29
pedv is a29
liu et al29
infection with the29
significant economic losses29
protein of porcine29
and weaned pigs29
need to be29
different concentrations of29
of the first29
then incubated with29
belonging to the29
performed using the29
pedv has been29
pedv strains in29
in agreement with29
s t protein29
needs to be29
in the feces29
a multiplicity of29
a result of29
in the swine29
propagated in vero29
were tested for29
and pedv s29
analysis based on29
and the cells29
cells were maintained29
the severity of29
weeks of age29
in central china29
orf a b29
no competing interests29
was removed and29
cell cultures and29
open reading frames29
of the intestinal29
with pedv and28
amino acid changes28
in which the28
at an moi28
sun et al28
of the cell28
n protein of28
the infection of28
cell culture medium28
log copies ml28
the onset of28
the highly virulent28
an moi of28
for up to28
the introduction of28
in the serum28
pedv was first28
and gds strains28
detection of the28
outbreaks of porcine28
diarrhoea virus in28
gds and gds28
is a functional28
structure of the28
is a major28
lower than that28
were detected by28
vaccinated with the28
the same time28
has also been28
at h after28
a vero cell28
small and large28
of the three28
with regard to28
room temperature for28
at the time28
other pedv strains28
there is a28
pedv s gene28
was detected by28
a receptor for28
united states porcine28
are needed to28
and evolution of27
in serum and27
in the early27
highly virulent pedv27
in figure a27
virus porcine epidemic27
is one of27
plates were washed27
was performed as27
of the most27
to investigate whether27
of ped in27
cells of the27
and pedv were27
and pathogenicity of27
has been used27
in the control27
were analyzed using27
infected with the27
of the genus27
villous epithelial cells27
was found in27
spike protein is27
four structural proteins27
old weaned pigs27
the s glycoprotein27
significant differences in27
were able to27
to analyze the27
the natural host27
virus and transmissible27
cells transfected with27
been identified in27
in england in27
identification of a27
diarrhea and vomiting27
difference in the27
supernatants were collected27
pigs at pid27
the expression levels27
vitro and in27
and then incubated27
of tbk and27
is an important27
was identified in27
the proportion of27
and the s27
be associated with27
i ifn production27
than those of27
at c for27
cells were co27
a and icpc27
inhibition of pedv27
to transmissible gastroenteritis27
in the natural27
were pretreated with27
interferon production by27
washed twice with27
in recent years27
orf gene of27
those in the27
rna was detected27
detection limit of27
and in vivo27
antibody responses in27
considered statistically significant27
was performed to27
diarrhea and dehydration27
between pedv and27
cv and lnct27
the control group26
results demonstrated that26
at days post26
hu et al26
each group were26
and porcine deltacoronavirus26
the jejunum and26
is possible that26
cell culture isolation26
of pedv to26
epithelial cells of26
spike protein genes26
tissue culture plates26
did not show26
infectious peritonitis virus26
log ge ml26
of the four26
transmission of pedv26
like particles in26
some of the26
play an important26
a pedv strain26
g a and26
these results suggest26
levels of pedv26
be involved in26
protein in the26
fragment length polymorphism26
pedv infection of26
accordance with the26
s indirect elisa26
comparison of the26
strain with a26
the epitope region26
induced by pedv26
activity of the26
the course of26
type iii interferon26
positive control group26
determination of the26
disease in pigs26
in the healthy26
extracted from the26
susceptible to pedv26
paraformaldehyde for min26
a review of26
influenza a virus26
of the samples26
supported by the26
has been demonstrated26
no conflict of26
s protein and26
was able to26
the pedv outbreak26
virus in porcine26
pedv infection was26
and then infected26
diarrhea in piglets26
isolated from the26
have no competing26
coronavirus nucleocapsid protein26
have been used26
pcr was performed26
cd t cell26
carried out in26
of diarrhea in26
molecular evolutionary genetics25
g a pedv25
the specificity of25
group a rotavirus25
korean pedv field25
pcr assay for25
the in vitro25
phylogenetic analysis based25
pedv strains and25
in southern china25
determined to be25
the immune response25
the endoplasmic reticulum25
and the results25
characteristics of the25
kweon et al25
of the us25
on vero cells25
assays were performed25
infection with pedv25
part of the25
apoptotic cell death25
included in the25
spread of the25
could be detected25
of each virus25
by pedv infection25
cells per well25
genetic diversity of25
of the partial25
at a concentration25
the possibility of25
s gene and25
in europe and25
the m gene25
were stored at25
serial dilutions of25
considered to be25
amino acid mutations25
region capable of25
the pcr products25
against the pedv25
heat and rnase25
positive and negative25
been used to25
for min to25
molecular epidemiology and25
severe watery diarrhea25
hepatitis c virus25
fcov type and25
the aim of25
against the porcine25
summarized in table25
to the s25
different time points25
acute pedv infection25
mortality in piglets25
restriction fragment length25
porcine nidovirus infection25
in china and25
in a humidified25
of the full25
nsp and nsp25
at rt and25
indicated that pedv25
of inducing neutralizing25
evolutionary genetics analysis25
korean pedv strains25
of the infected25
of pedv has25
demonstrated that pedv25
a recent study25
calculated using the24
of the viruses24
as described in24
proof of concept24
presence of the24
the lamina propria24
mouth disease virus24
strain isolated in24
in pedv infection24
presence or absence24
different types of24
in weaned pigs24
the porcine apn24
were performed in24
crude extract containing24
type iii ifn24
virus in cell24
at a multiplicity24
prior to the24
watery diarrhea and24
of the host24
all samples were24
from each group24
of the sars24
oh et al24
was assessed by24
united states and24
of pedv replication24
pcr and western24
by using the24
study showed that24
assay for the24
porcine group a24
the gastrointestinal tract24
completion of the24
culture supernatants were24
virulence of pedv24
after the first24
differential detection of24
the identification of24
against pedv in24
as a vehicle24
of the recombinant24
the supernatants were24
like particles associated24
sequencing of the24
of the ifn24
was defined as24
neutralizing antibodies in24
particles associated with24
crystal structure of24
of a recombinant24
were blocked with24
chang et al24
cleavage of the24
with paraformaldehyde for24
korean field strains24
the infected cells24
the study of24
pedv s proteins24
porcine apn transgenic24
us pedv strains24
outbreaks of ped24
was determined using24
and approved the24
at rt for24
experiments were performed24
for the simultaneous24
or absence of24
vero cell monolayers24
assay was performed24
pedv replication in24
mortality in suckling24
culture isolation and24
tbk or irf24
virus nucleocapsid protein24
the nucleotide sequences24
have not been24
clinical signs were24
the host innate24
be used as24
in north america24
higher than those24
performed as described24
caused by porcine24
the sars coronavirus24
were tested in24
the culture medium23
using graphpad prism23
higher than the23
data indicated that23
of the cells23
the simultaneous detection23
we investigated the23
used to determine23
a source of23
ml of pedv23
at a moi23
intact s gene23
neutralizing antibody titers23
was performed in23
the random forest23
a final concentration23
can be divided23
was performed by23
this study are23
in terms of23
an ion channel23
was isolated from23
and stained with23
the genome of23
china complete genome23
with pedv strains23
in infected cells23
the coronaviridae family23
i interferon production23
of the outbreak23
the loss of23
was analyzed by23
particles in japan23
were then washed23
entry into cells23
with transmissible gastroenteritis23
diagnosis of pedv23
in the nucleus23
the gds and23
genetic characterization of23
within the same23
minimum essential medium23
are listed in23
a series of23
and porcine respiratory23
epidemiology of porcine23
the proliferation of23
of prrsv and23
novel porcine epidemic23
of the isolate23
well plates and23
insertions and deletions23
reported in the23
us pedv strain23
a novel strain23
culture medium was23
the cellular receptor23
emerging epizootic swine23
the s subunit23
compared to those23
porcine enteric coronaviruses23
of the assay23
a and g23
as determined by23
of peptide h23
relationship between the23
the ica kit23
is a member23
function of the23
have been developed23
insights into the23
was performed with23
epitope region capable23
on pedv replication23
the release of23
significantly lower than23
no effect on23
positive cells in23
the vero cell23
of the same23
and orf b23
n gene of23
outbreak of swine23
ranging from to23
that the s23
immune response in23
antibodies were detected23
sequence of porcine23
is important for23
the day of23
could not be23
in china in23
obtained from a23
by indirect elisa23
and immunogenicity of23
of the epitope23
be related to22
and analysis of22
of the trial22
to explore the22
at the indicated22
statistical analysis was22
of aminopeptidase n22
could be used22
in the p22
acid binding activity22
the importance of22
to be the22
prior to farrowing22
the entire genome22
huge economic losses22
approved the final22
mucosal and systemic22
as seen in22
similar to us22
the us strains22
was propagated in22
the cytoplasm of22
authors declare no22
of infected cells22
an intact s22
the survival of22
can be found22
cellular receptor for22
and attenuation of22
to amplify the22
pedv specific siga22
new syndrome of22
for clinical signs22
states porcine epidemic22
infection of pedv22
antibodies in serum22
m and n22
of suckling piglets22
first time in22
removed and the22
a moi of22
the heat and22
divided into two22
and rnase treated22
with a high22
into vero cells22
suckling and weaned22
more than of22
of a vero22
spread of porcine22
in the dark22
and western blot22
nk cell activity22
to examine the22
the plasma membrane22
of vero cell22
diarrhea in china22
swine farms in22
prior to inoculation22
was applied to22
diarrhea virus date22
was calculated using22
duration of the22
the risk of22
s or s22
the in vivo22
mortality in neonatal22
syndrome of porcine22
the reemerging korean22
multiple sequence alignment22
analysis showed that22
because of the22
all pigs were22
differentially expressed genes22
pedv at a22
than that in22
fecal viral rna22
in the jejunum22
united states in22
were euthanized at22
gerber et al22
adaptation in vitro22
circulating in the22
nk cell frequencies22
or s t22
associated with coronavirus22
the pathogenicity of22
evaluation of a22
reported to be22
genbank accession number22
to study the22
is shown in22
used as an21
b and c21
the pedv coronavirus21
in fecal samples21
s domain of21
was reported in21
an evaluation of21
ohio state university21
were harvested and21
cells were pre21
of the protein21
analysis of orf21
from three independent21
to us strains21
were located in21
found that pedv21
were isolated from21
in the family21
of swine diarrhea21
g b strains21
rectal swabs were21
no significant difference21
the interaction of21
study demonstrated that21
ec cells in21
type iii ifns21
virus shedding in21
outbreak of diarrhea21
pedv n and21
clarified by centrifugation21
focused on the21
was shown to21
proteins in the21
apn transgenic mice21
and large intestines21
serial propagation of21
the ohio state21
source of the21
a and orf21
growth adaptation in21
gilts and sows21
of porcine circovirus21
virulence in vivo21
pedv in vero21
blood samples were21
we demonstrated that21
was based on21
virus strains similar21
strains similar to21
signs of diarrhea21
could be a21
on pedv infection21
in serum samples21
the first report21
tissue culture infectious21
suggests that the21
in situ hybridization21
of cell culture21
our results showed21
pigs at pids21
are presented as21
of s proteins21
pedv s or21
of the different21
were visualized using21
the viral envelope21
of porcine enteric21
is consistent with21
to the pedv21
were used in21
may not be21
of the authors21
and orf genes21
herpes simplex virus21
primers and probes21
be detected in21
antibodies in the21
discovery of a21
portion of the21
of pedv on21
immune responses to21
into three groups21
that in the21
between the pedv21
of q r21
in vitro prophylactic21
of pedv strain21
ml of the21
fcov type s21
in ml of21
virus and its21
on the pedv20
related porcine epidemic20
in the host20
the suppression of20
analysis of genetically20
in the cells20
crypt epithelial cells20
and fecal samples20
are summarized in20
type associated with20
genetically diverse us20
analysis indicated that20
tgev and prcv20
the pedv infection20
cells were pretreated20
in each group20
were washed twice20
characterized by vomiting20
in the cell20
piglets in group20
the final manuscript20
mortality rates in20
activity of pedv20
the vaccine strain20
of pedv pl20
the type i20
of genetically diverse20
high mortality rates20
divided into four20
spread of pedv20
human immunodeficiency virus20
pedv is an20
field strains by20
were mixed with20
and pathogenesis of20
the icpc a20
are involved in20
including a novel20
terminus of the20
the most abundant20
induced by the20
diarrhea of a20
which is the20
to the us20
the small and20
with bovine serum20
apparently new syndrome20
isolation and sequence20
the structural proteins20
were orally inoculated20
in pbs for20
diverse us porcine20
shown that the20
was first detected20
was subjected to20
transfected with the20
s protein in20
inhibited pedv infection20
of the fusion20
the sequences of20
culture infectious dose20
villous atrophy in20
development of an20
treated with the20
swine diarrhea of20
provided by the20
an apparently new20
the vaccine strains20
to the pig20
the mechanisms of20
to those in20
of this virus20
and functional characterization20
due to its20
replication in the20
pc a strain20
serum and colostrum20
cov pl pro20
diarrhea in canada20
and used for20
the presence or20
swine fever virus20
cells were lysed20
debouck and pensaert20
cell adapted porcine20
sequences of pedv20
analyses were performed20
novel strain with20
and infected with20
analyses of the20
regions of the20
serum samples from20
entry of porcine20
sequence of pedv20
complete s gene20
strains including a20
suckling pigs had20
the ability to20
large number of20
length polymorphism analysis20
diarrhea virus by20
of innate immune20
virus strains including20
genomic rna and20
in nursing piglets20
new ica was20
simultaneous detection and20
with other pedv20
it is also20
nucleotide sequence of20
survival of pedv19
expression level of19
with the virus19
h prior to19
in us swine19
alphacoronavirus and betacoronavirus19
and the other19
results suggested that19
by restriction fragment19
diarrhea virus dr19
mrna levels of19
there was a19
and g b19
introduction of pedv19
data were analyzed19
swine porcine epidemic19
the sequence of19
contaminated complete feed19
to be an19
antiviral activity against19
with the virulent19
ion channel protein19
was measured by19
serum samples collected19
virulent strain of19
the results were19
differentiation of a19
gammacoronavirus and deltacoronavirus19
leading to the19
from korean field19
piglets from the19
of alphacoronavirus and19
with virulent pedv19
in of the19
of ch jx19
the dna damage19
were collected for19
was identified as19
to the nucleus19
spike glycoprotein gene19
korean field isolates19
with an intact19
transferred to a19
which is a19
in porcine intestinal19
with the n19
review of current19
modified eagle medium19
and negative controls19
viral rna shedding19
in the genus19
large numbers of19
lower than those19
diseases of swine19
epithelial cells and19
original us pedv19
propagation of porcine19
of pedv with19
de arriba et19
in pregnant sows19
of attenuated porcine19
and sg mrna19
after infection with19
seen in figure19
the intestinal mucosa19
strains by restriction19
the evolution of19
of pedv from19
our previous study19
to elucidate the19
been identified as19
to understand the19
and cloned into19
early stage of19
of current epidemiology19
infection in pigs19
the us and19
carvajal et al19
and centrifuged at19
virus from korean19
diarrhoea virus and19
of mouse hepatitis19
terminal region of19
of pedv nsp19
incubated with pedv19
the feces of19
the genus deltacoronavirus19
arriba et al19
of pdcov in19
to each other19
nucleotide and amino19
the korean pedv19
a cellular receptor19
a highly contagious19
to the nucleolus19
diarrhea virus field19
of the reemerging19
and that the19
the mixture was19
amino acid residues19
for an additional19
as a template19
vero cell adapted19
h at c19
and available vaccines19
not significantly different19
epidemiology and available19
during the first19
read and approved19
polymorphism analysis of19
is a type19
in the ipec19
rna in serum19
acute diarrhea in19
than those in19
tcid ml of19
the virus and19
pedv and the19
was first identified19
swabs were collected19
was significantly lower19
vero cells grown19
funded by the19
incubated for min19
of s gene19
a type i19
was to determine19
resulted in the19
at and dpi19
pigs porcine epidemic19
pedv reference strains19
of sows with19
in this group19
the s indel19
inhibitory effects of19
of hnrnp a19
were cloned into19
current epidemiology and19
in the future19
further studies are19
diarrhea virus porcine19
is the main19
epidemic diarrhoea coronavirus19
based on these19
the detection and19
of virulence in19
prevention and control19
of other coronaviruses19
caused by a18
indicates that the18
a cause of18
attenuation of virulence18
polyacrylamide gel electrophoresis18
the coronavirus spike18
role of the18
claims in published18
remains neutral with18
surface s proteins18
daily for clinical18
and serial propagation18
hou et al18
were measured by18
each of the18
to jurisdictional claims18
in published maps18
pedv rna shedding18
had the highest18
of the sows18
the mortality rate18
were inoculated orally18
was similar to18
arch virol doi18
that n protein18
weaned pigs at18
s and n18
of amino acids18
difference between the18
of blfp crude18
would like to18
replaced with fresh18
nature remains neutral18
of sars coronavirus18
the assay was18
limit of the18
diarrhea in pigs18
maps and institutional18
substantial economic losses18
length s gene18
that can be18
the regulation of18
a critical role18
were considered statistically18
the difference in18
in significant economic18
research and development18
vero cell attenuated18
the beginning of18
with different concentrations18
in both the18
n protein and18
isolation and serial18
neutral with regard18
to the cytoplasm18
vero cells at18
and treated with18
each well and18
h at room18
functional characterization of18
were no significant18
during the early18
that pedv entry18
studies showed that18
vero cells infected18
than of the18
of fcov type18
terminal domain of18
vlasova et al18
the virus is18
infection with a18
the viral rna18
c in a18
the cv strain18
and g a18
identified as the18
assays for the18
germany and the18
cells as described18
the membrane was18
under field conditions18
the activity of18
inoculated gn pigs18
results revealed that18
the united kingdom18
to the genus18
the university of18
the intestinal contents18
and institutional affiliations18
of the gene18
gene in the18
induction of neutralizing18
and partial characterization18
targeted rna recombination18
in spike protein18
of the animals18
evaluation of the18
of global porcine18
the inoculum was18
jurisdictional claims in18
extracts of ln18
with growth adaptation18
diarrhea viruses from18
using monoclonal antibodies18
s proteins were18
amount of pedv18
virus is a18
glycoprotein gene of18
for pedv detection18
in the g18
from the united18
in piglets in18
europe and asia18
to determine if18
virulence of the18
the piglets were18
by reverse transcription18
against the s18
and permeabilized with18
analysis of a18
jnk and erk18
comparison of porcine18
of the virulent18
these data indicate18
tryptose phosphate broth18
structural and functional18
piglets less than18
contributed to the18
of these viruses18
in pigs and18
was present in18
experimentally infected with18
to the editor18
sodium dodecyl sulfate18
severe clinical signs18
vero cells as18
mammalian and avian18
the possibility that18
as a cause18
the impact of18
in pedv infected18
infected vero e18
inhibitory effect of18
this suggests that18
severe atrophic enteritis18
an indirect elisa18
published maps and18
onset of clinical18
were confirmed by18
in cultured cells18
replication and pathogenesis18
as a receptor18
indel pedv strain18
for pedv rna18
supernatants were harvested18
cell entry of18
are associated with18
aminopeptidase n and18
antigenic relationships among18
regard to jurisdictional18
yuan et al18
pedv at an18
the cell lysates18
to verify the18
the findings of18
mediated by the18
cells were detected18
virus in south18
springer nature remains18
at dpi and18
pedv strains from18
the disease was18
endoplasmic reticulum stress18
a class i18
global porcine epidemic18
heterogeneity in spike18
first identified in18
a vehicle for18
partial characterization of18
pedv replication and18
cells were stained18
identified in this18
of the entire18
data suggest that18
serve as a18
been shown that18
and the pedv18
s genes of18
of diarrhea and18
could be observed17
the cleavage of17
viral replication in17
are shown as17
to the family17
is known to17
or infected with17
using vero cells17
in a previous17
equal volume of17
from the same17
cultures and partial17
currently circulating in17
veterinary diagnostic laboratory17
role of transportation17
vitro and attenuation17
detection of transmissible17
blocked with bovine17
the porcine aminopeptidase17
significant difference between17
in amino acid17
bat coronaviruses as17
up to days17
pan et al17
with those of17
interfere with the17
in comparison with17
after inoculation with17
virus shedding and17
was performed on17
associated with porcine17
i and type17
class i virus17
and evaluation of17
to the original17
china isolation and17
identity with the17
were as follows17
cells and ipec17
source of alphacoronavirus17
collected at hpi17
yc pedv strain17
infection in vero17
is a class17
is not a17
that there were17
prevent and control17
to weaned pigs17
content of the17
associated with growth17
that pedv is17
and has been17
large amounts of17
supports bat coronaviruses17
limit of detection17
like to thank17
in the virus17
gene and the17
of pedv by17
outbreak of pedv17
cd t cells17
dmem supplemented with17
diarrhea virus nucleocapsid17
and type iii17
levels of the17
compared with ifn17
the two pedv17
i virus fusion17
the proteins were17
immune responses by17
and molecular epidemiology17
protective efficacy of17
orf genes of17
were then incubated17
the application of17
avian coronaviruses in17
discovery of seven17
associated lymphoid tissues17
is necessary to17
performed in triplicate17
and analyzed by17
coronavirus spike proteins17
of gammacoronavirus and17
were selected for17
were centrifuged at17
with pedv in17
a fluorescence microscope17
at least one17
was mixed with17
and further sequence17
of pedv into17
hematoxylin and eosin17
was calculated as17
and s t17
enteric disease in17
corresponding to the17
virus was detected17
observed between the17
of seven novel17
quantification of transmissible17
determined by a17
rt for min17
source of gammacoronavirus17
avian coronaviruses as17
relative to the17
further sequence analysis17
by the addition17
with each other17
novel mammalian and17
in the nucleolus17
reported that the17
were challenged with17
from germany and17
virulent pedv strain17
contribution of the17
deltacoronavirus supports bat17
and can be17
g b pedv17
of cellular cholesterol17
and comparison of17
log tcid ml17
in asia and17
real time rt17
the virus to17
genome copies ml17
absence of trypsin17
level of the17
higher in the17
compared to that17
epithelium of the17
only a few17
the invasion rates17
high morbidity and17
porcine alveolar macrophages17
coronaviruses in the17
cell attenuated porcine17
protection against pedv17
pedv infection is17
rna in the17
the transmissible gastroenteritis17
in pigs in17
supported by a17
s t proteins17
natural feeding behavior17
confirmed that the17
n protein with17
virus fusion protein17
followed by cycles17
strains from the17
addition to the17
of pigs in17
aim of this17
for the duration17
virus in italy17
insight into the17
samples stored at17
for viral replication17
used to amplify17
the clinical signs17
performed using a17
madson et al17
encodes an ion17
inoculated with a17
at pids and17
the p group17
pigs compared to17
response to pedv17
h at rt17
and betacoronavirus and17
to develop a17
thought to be17
epidemiology of pedv17
to the global17
in the viral17
the membranes were17
cells grown in17
to the control17
inoculum was removed17
fecal samples from17
was kindly provided17
infectivity of pedv17
the cytoplasm and17
viruses from germany17
vaccines for porcine17
the early stage17
in the pathogenesis17
western blotting analysis17
was added and17
pedv and pdcov17
on day post17
vaccines have been17
virus strain from17
seven novel mammalian17
to compare the17
the culture supernatants17
authors read and17
essential for the17
characterized by acute17
cloning and further17
betacoronavirus and avian17
immune responses in17
virus titers were17
from the us17
was collected from17
of pedv hljby17
in europe in17
of this article17
required for the16
the reciprocal of16
that there is16
were present in16
numbers of pedv16
the proportions of16
protein and the16
number of pigs16
the fusion core16
disease characterized by16
coe and rlppg16
numbers of serotonin16
phylogenetic trees were16
and found that16
effects of the16
vet res doi16
s gene were16
fusion of the16
stranded rna virus16
and cultured in16
been reported that16
as the reciprocal16
in the previous16
western blot and16
in group were16
signs of ped16
debouck et al16
evolution of the16
diarrhoea caused by16
as a control16
pedv infection by16
binding to the16
medium supplemented with16
be used for16
hpi and hpi16
and genetic characterization16
of three independent16
not detected in16
the mesenteric lymph16
and specificity of16
a novel porcine16
that pedv nsp16
were separated by16
in swine porcine16
a lack of16
targeting the s16
within the s16
nuclei were stained16
that did not16
fusion core complex16
h after transfection16
the recombinant protein16
determined by the16
in conventional pigs16
of the order16
pedv strain pc16
pbs for min16
amino acid positions16
all authors read16
at pid and16
and cells were16
as a source16
h post infection16
letter to the16
in the negative16
coronavirus related to16
studies are needed16
murine hepatitis virus16
rna genome of16
the sialic acid16
were measured using16
suggested that pedv16
of the data16
and then the16
it can be16
porcine deltacoronavirus in16
seven open reading16
an equal volume16
of the proteins16
virus replication in16
infectivity of icpc16
in the sera16
groups a and16
were calculated using16
a consequence of16
contaminated feed as16
apn positive cells16
prrsv or pedv16
regulates virus production16
but not the16
virus infection and16
the results indicated16
pedv g b16
and pedv infection16
the analysis of16
was lower than16
of the inoculated16
virus infection of16
strains isolated in16
cells with pedv16
pl pro s16
were consistent with16
swine industry in16
phosphate buffered saline16
and used as16
science and technology16
was approved by16
the results are16
the structure of16
by cycles of16
was expressed as16
fecal virus shedding16
defined as the16
genbank accession numbers16
strains have been16
epithelial cell line16
watery diarrhea in16
virus associated with16
revealed that pedv16
along with the16
channel protein and16
data showed that16
in south china16
had no effect16
diarrhea virus genome16
across the country16
increase in the16
to be more16
the inhibitory effects16
high mortality rate16
such as pedv16
old piglets were16
for at least16
of the experiment16
epidemic of diarrhoea16
in viral replication16
monitored daily for16
strains in china16
is based on16
virus replication and16
not in the16
diarrhea virus isolate16
of spike gene16
proteolytic cleavage of16
phylogenetic tree was16
the nucleus and16
twice with pbs16
deposited in genbank16
were determined using16
n protein in16
that pedv n16
was visualized using16
rate of the16
was evaluated by16
apn negative cells16
with cell culture16
and control groups16
the titer of16
the binding of16
variant strain of16
using trizol reagent16
protein gene of16
with some modifications16
immune response to16
infected or infected16
with bound tgev16
pedv classical attenuated16
be responsible for16
was replaced with16
of two novel16
until the end16
and serum samples16
divided into three16
positive cells were16
and vaccine strains16
and control of16
attenuated vaccine strain16
the pigs were16
length genome sequence16
to control the16
infection of vero16
pedv entry into16
a cell culture16
the mechanism of16
pedv antibodies in16
was not detected16
critical role in16
the duplex rt16
the occurrence of16
in the colostrum16
of diarrhoea caused16
the piglets in16
sows with a16
were exposed to16
of the other15
concentrations of the15
a nitrocellulose membrane15
been used as15
antiviral innate immune15
the source of15
the crude extract15
involved in pedv15
of the villi15
cv and dr15
titer in the15
pedv strains at15
infected with a15
the inhibitory effect15
rna and sg15
as a viral15
a final extension15
of virus shedding15
to our knowledge15
of pedv field15
parts of the15
a virulent pedv15
for rapid detection15
in all groups15
in the gastrointestinal15
nucleotide sequence identity15
the origin of15
novel swine enteric15
of transportation in15
and regulates virus15
and application of15
the plate was15
pedv and other15
the content of15
the partial spike15
vaccine strain cv15
the host cells15
after washing with15
of less than15
for h prior15
caused by the15
in a single15
derived from the15
for induction of15
weaned pigs had15
has shown that15
and porcine group15
proteolytic activation of15
and virulence of15
of human coronavirus15
graphpad prism software15
tested in the15
clinical disease and15
orally immunized with15
may be a15
virulent pedv strains15
epitopes of the15
proteins have been15
genome of the15
specificity and sensitivity15
values of the15
times with pbst15
shown in the15
immune response against15
c for s15
of the method15
sense rna virus15
method for the15
pigs had significantly15
and protective efficacy15
the differences between15
density to porcine15
used to infect15
as vaccine candidate15
the molecular epidemiology15
gene expression of15
were observed between15
indirect elisa was15
role in pedv15
in the regulation15
innate immune system15
first detected in15
the us in15
epidermal growth factor15
p and p15
was conducted using15
in the development15
from the pedv15
in the lamina15
activation of nf15
in spread of15
filtered through a15
region of pedv15
reciprocal of the15
together with the15
pedv isolates and15
of the negative15
basis for the15
new insights into15
i and iii15
on ice for15