quadgram

This is a table of type quadgram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

quadgram	frequency
porcine epidemic diarrhea virus	2208
of porcine epidemic diarrhea	1105
epidemic diarrhea virus in	321
porcine epidemic diarrhoea virus	277
in the united states	244
severe acute respiratory syndrome	157
epidemic diarrhea virus infection	140
porcine reproductive and respiratory	134
reproductive and respiratory syndrome	133
for the detection of	129
in the presence of	127
the porcine epidemic diarrhea	125
epidemic diarrhea virus strains	125
acute respiratory syndrome coronavirus	119
of the porcine epidemic	119
porcine epidemic diarrhea viruses	119
and respiratory syndrome virus	119
of porcine epidemic diarrhoea	118
porcine epidemic diarrhea in	115
of the s protein	109
complete genome sequence of	106
epidemic diarrhea virus strain	104
in the present study	93
epidemic diarrhea virus and	86
detection of porcine epidemic	83
diarrhea virus in the	81
emergence of porcine epidemic	81
as shown in fig	78
strain of porcine epidemic	75
associated with diarrhea in	73
characterization of porcine epidemic	70
virus in the united	70
and phylogenetic analysis of	68
us porcine epidemic diarrhea	67
isolation and characterization of	66
with diarrhea in swine	66
transmissible gastroenteritis virus and	65
the results showed that	63
review of molecular epidemiology	63
sequence analysis of the	63
a comprehensive review of	62
comprehensive review of molecular	62
the pedv s protein	62
particle associated with diarrhea	62
genome sequence of a	61
and viral genomic sequences	61
like particle associated with	60
gene of porcine epidemic	60
and porcine epidemic diarrhea	59
used in this study	59
gastroenteritis virus and porcine	59
an important role in	59
the end of the	59
cells were infected with	58
of transmissible gastroenteritis virus	57
washed three times with	56
of the s gene	56
genes of porcine epidemic	55
variants of porcine epidemic	55
were infected with pedv	55
phylogenetic analysis of porcine	54
for porcine epidemic diarrhea	54
against porcine epidemic diarrhea	53
diarrhea virus in china	53
in the s gene	53
the s protein of	53
on the other hand	52
as shown in figure	52
with porcine epidemic diarrhea	52
epidemic diarrhea virus spike	51
analysis of porcine epidemic	51
samples were collected from	50
new variants of porcine	50
outbreak of porcine epidemic	49
o u r n	49
u r n a	49
j o u r	49
r n a l	49
virus and porcine epidemic	49
r o o f	48
cells were transfected with	48
a l p r	48
p r o o	48
the cells were washed	48
l p r e	48
was used as a	48
n a l p	48
cells were treated with	48
in the spike gene	47
diarrhea virus spike protein	47
in vero e cells	46
the presence of pedv	46
the detection of pedv	46
a member of the	45
epidemic diarrhea virus from	44
in the small intestine	44
of pedv in the	44
rna was extracted from	44
of severe acute respiratory	44
cells were incubated with	42
and characterization of porcine	42
variant porcine epidemic diarrhea	42
as well as the	42
in the absence of	42
of the spike protein	42
attenuated porcine epidemic diarrhea	42
at the end of	41
the authors declare that	41
pigs of all ages	41
of the small intestine	41
virus strains in the	41
aminopeptidase n is a	41
genotyping of emergent porcine	40
of emergent porcine epidemic	40
strains in the united	40
the detection of porcine	40
propagation of the virus	40
and genotyping of emergent	40
characterization and phylogenetic analysis	39
molecular characterization and phylogenetic	39
strain pc a in	39
african green monkey kidney	39
diarrhea viruses associated with	39
a porcine epidemic diarrhea	39
epidemic diarrhea viruses associated	39
emergent porcine epidemic diarrhea	39
on the surface of	39
were observed in the	39
diarrhea virus strains in	39
cells were fixed with	39
and transmissible gastroenteritis virus	39
were washed three times	39
incubated for h at	39
by porcine epidemic diarrhea	38
evolution of pedv strains	38
porcine epidemic diarrhea coronavirus	38
of porcine reproductive and	38
to porcine epidemic diarrhea	38
of us porcine epidemic	37
viruses associated with the	37
the virus of porcine	37
and complex evolution of	37
of the virus of	37
economic losses to the	37
virus strain pc a	37
diarrhea in suckling piglets	37
in the s protein	37
virus of porcine epidemic	37
experimental infection of pigs	37
min at room temperature	37
complex evolution of pedv	37
with fetal bovine serum	37
characteristics and complex evolution	36
for min at room	36
swine in the united	36
the gene source of	36
middle east respiratory syndrome	36
diarrhea virus strain pc	36
associated with the disease	36
porcine transmissible gastroenteritis virus	36
strains of porcine epidemic	36
for the presence of	36
diarrhea in cell culture	36
distinct characteristics and complex	36
epidemic diarrhea in cell	36
with the disease outbreak	36
epidemic diarrhea in suckling	35
disease outbreak among swine	35
the porcine epidemic diarrhoea	35
among swine in the	35
outbreak among swine in	35
the disease outbreak among	35
has been shown to	34
as the gene source	34
was added to each	34
coronaviruses as the gene	34
care and use committee	34
for the first time	34
total rna was extracted	34
animal care and use	34
pensaert and de bouck	33
added to each well	33
and sequence analysis of	33
adapted porcine epidemic diarrhea	33
the spike gene of	33
diarrhea viruses isolated in	33
protein genes of porcine	33
three times with pbs	33
that they have no	33
on the cell surface	33
for the development of	33
epidemic diarrhea viruses isolated	33
of a porcine epidemic	33
the s gene of	33
variant of porcine epidemic	32
hek t cells were	32
the pedv n protein	32
plays an important role	32
declare that they have	32
results showed that the	32
authors declare that they	32
vero e cells were	32
play a role in	31
cells were seeded in	31
institutional animal care and	31
a new porcine enteric	31
was added to the	31
a functional receptor for	31
were detected in the	31
pc a in gnotobiotic	31
cells were inoculated with	31
east respiratory syndrome coronavirus	31
this is the first	31
was used as the	31
new porcine enteric coronavirus	31
pcd and vero cells	30
infected with pedv at	30
and vero cells were	30
the spike protein of	30
large deletion in the	30
spike gene of porcine	30
the antiviral activity of	30
of this study was	30
similar to that of	30
like strain of porcine	30
pathology of us porcine	30
studies have shown that	30
porcine aminopeptidase n is	30
isolation of porcine epidemic	30
on the basis of	30
supplemented with fetal bovine	30
a in gnotobiotic pigs	30
viruses isolated in korea	30
was found to be	29
on porcine epidemic diarrhea	29
infected with porcine epidemic	29
the intracellular extracts of	29
for min at rt	29
a variant porcine epidemic	29
the s protein is	29
was first reported in	29
the severe acute respiratory	29
for h and then	29
the small intestine of	29
s protein of pedv	29
amino acid sequences of	29
the recombinant s protein	28
a large deletion in	28
and the cells were	28
with a large deletion	28
the nucleocapsid protein of	28
these results indicated that	28
the sensitivity of the	28
this work was supported	28
in pigs of all	28
this study was to	28
a multiplicity of infection	28
at room temperature for	28
functional receptor for the	28
the presence of trypsin	28
new variant of porcine	28
gds and gds strains	28
the results of the	28
of the orf gene	28
epidemic diarrhoea virus in	28
a and icpc a	27
pigs with a new	27
as a negative control	27
cells were washed with	27
antibodies against porcine epidemic	27
at an moi of	27
with a new porcine	27
work was supported by	27
in vitro and in	27
infection of pigs with	27
type i ifn production	27
virus and transmissible gastroenteritis	27
of pigs with a	27
is a functional receptor	27
these results indicate that	27
and characterization of a	27
the s and s	27
icpc a and icpc	27
the expression levels of	26
were incubated for h	26
detection and quantification of	26
it is possible that	26
of tbk and irf	26
was detected in the	26
feline infectious peritonitis virus	26
neutralizing antibodies against the	26
the cells were fixed	26
of a variant porcine	26
in the natural host	26
cells infected with pedv	26
is one of the	26
in the current study	26
to the swine industry	26
they have no competing	26
in accordance with the	26
classical attenuated vaccine strains	26
outbreaks of porcine epidemic	26
porcine intestinal epithelial cells	25
phylogenetic analysis based on	25
detection of antibodies against	25
of transmissible gastroenteritis coronavirus	25
phylogenetic analysis of the	25
of the nucleocapsid protein	25
play an important role	25
and the united states	25
region capable of inducing	25
of inducing neutralizing antibodies	25
sequence of a porcine	25
then infected with pedv	25
at a concentration of	25
these results suggest that	25
vero cells were infected	25
deletion in the spike	25
vitro and in vivo	25
molecular evolutionary genetics analysis	25
against the porcine epidemic	25
similar to those of	25
cd cd t cell	25
well tissue culture plates	25
of pedv rna in	25
than that of the	25
of the pedv s	25
restriction fragment length polymorphism	25
we found that the	25
the amino acid sequence	24
the united states and	24
korean pedv field isolates	24
complete genome sequences of	24
like particles associated with	24
presence or absence of	24
inducing neutralizing antibodies against	24
the detection limit of	24
cells were cultured in	24
the jejunum and ileum	24
protein of porcine epidemic	24
of pedv n protein	24
have no competing interests	24
and then infected with	24
antibodies against the porcine	24
cell culture isolation and	24
the pedv spike protein	24
capable of inducing neutralizing	24
the united states porcine	24
at the same time	23
genome sequence of porcine	23
one of the most	23
epitope region capable of	23
emerging epizootic swine virus	23
with paraformaldehyde for min	23
type i interferon production	23
identification of the epitope	23
the cells were then	23
an emerging and re	23
cells were grown in	23
caused by porcine epidemic	23
no conflict of interest	23
the epitope region capable	23
was significantly higher than	23
in this study were	23
the presence of the	23
propagated in vero cells	23
of prrsv and pedv	23
economic losses in the	23
is a member of	23
of the epitope region	23
the gds and gds	23
with porcine epidemic diarrhoea	23
at a multiplicity of	23
an outbreak of swine	23
for the simultaneous detection	23
g a and g	23
china complete genome sequence	22
to that of the	22
of a vero cell	22
or s t protein	22
important role in the	22
epithelial cells of the	22
results indicated that the	22
of the n gene	22
as a result of	22
epidemic diarrhea virus date	22
were significantly higher than	22
were fixed with paraformaldehyde	22
the heat and rnase	22
heat and rnase treated	22
novel porcine epidemic diarrhea	22
the innate immune response	22
the cells were collected	22
a final concentration of	22
syndrome of porcine epidemic	22
new syndrome of porcine	22
like particles in japan	22
g for min at	22
united states porcine epidemic	22
can be divided into	22
sialic acid binding activity	22
were positive for pedv	22
the cells were incubated	21
and porcine respiratory coronavirus	21
the vero cells and	21
deduced amino acid sequences	21
strain with a large	21
the orf gene of	21
the partial s gene	21
at the time of	21
pcr and western blotting	21
the first time in	21
strains similar to us	21
n is a functional	21
virus strains similar to	21
and high mortality in	21
receptor for the pedv	21
for the pedv coronavirus	21
in the cytoplasm of	21
at different time points	21
the authors declare no	21
were negative for pedv	21
spread of porcine epidemic	21
similar to us strains	21
s domain of the	21
based on the s	21
the ohio state university	21
the n gene of	21
growth adaptation in vitro	21
an intact s gene	21
diarrhea virus strains similar	21
are listed in table	21
genetically diverse us porcine	20
the expression of the	20
morbidity and mortality in	20
pedv s or s	20
of swine diarrhea of	20
culture isolation and sequence	20
related porcine epidemic diarrhea	20
epidemiology of porcine epidemic	20
epidemic diarrhea in canada	20
vero cells were seeded	20
of tgev and pedv	20
virus strains including a	20
a and orf b	20
at c for h	20
analysis of genetically diverse	20
novel strain with a	20
s or s t	20
tissue culture infectious dose	20
isolation and sequence analysis	20
diarrhea virus strains including	20
sequence analysis of genetically	20
including a novel strain	20
be due to the	20
porcine apn transgenic mice	20
of pedv pl pro	20
completion of the porcine	20
of the virus in	20
fixed with paraformaldehyde for	20
approved the final manuscript	20
in the presence or	20
the plates were incubated	20
an apparently new syndrome	20
and approved the final	20
cell adapted porcine epidemic	20
a novel strain with	20
our results showed that	20
porcine group a rotavirus	20
apparently new syndrome of	20
the positive control group	20
analysis was performed using	20
cells were pretreated with	20
epidemic diarrhea virus by	20
fragment length polymorphism analysis	20
outbreak of swine diarrhea	20
of genetically diverse us	20
swine diarrhea of a	20
with bovine serum albumin	20
strains including a novel	20
the small and large	20
the united states in	20
shown in figure a	19
the presence or absence	19
polymorphism analysis of orf	19
as shown in table	19
from korean field strains	19
was observed in the	19
spike protein is a	19
statistical analysis was performed	19
it has been reported	19
vero cells were grown	19
length polymorphism analysis of	19
current epidemiology and available	19
type associated with coronavirus	19
simultaneous detection and quantification	19
epidemic diarrhea virus dr	19
nucleotide and amino acid	19
pedv in vero cells	19
to transmissible gastroenteritis virus	19
nucleotide sequences of the	19
diarrhea virus from korean	19
of current epidemiology and	19
used as a negative	19
a vero cell adapted	19
porcine epidemic diarrhoea coronavirus	19
orf a and orf	19
differentiation of a vero	19
of mouse hepatitis virus	19
epidemiology and available vaccines	19
molecular epidemiology of porcine	19
strains by restriction fragment	19
of attenuated porcine epidemic	19
entry of porcine epidemic	19
the negative control group	19
mortality in neonatal piglets	19
domain of the porcine	19
epidemic diarrhea virus field	19
a review of current	19
member of the genus	19
by restriction fragment length	19
lower than that of	19
at a moi of	19
read and approved the	19
pcr for the simultaneous	19
review of current epidemiology	19
of alphacoronavirus and betacoronavirus	19
epidemic diarrhoea virus and	19
diverse us porcine epidemic	19
analysis based on the	19
as seen in figure	19
host innate immune response	19
virus from korean field	19
of the spike gene	19
suckling and weaned pigs	19
epidemic diarrhea virus porcine	19
the simultaneous detection and	19
korean field strains by	19
the causative agent of	19
sequence of porcine epidemic	19
de arriba et al	19
vero cell adapted porcine	19
field strains by restriction	19
from three independent experiments	19
the duration of the	18
to jurisdictional claims in	18
of global porcine epidemic	18
nature remains neutral with	18
spike protein genes of	18
claims in published maps	18
diarrhea virus in south	18
springer nature remains neutral	18
of blfp crude extract	18
intracellular extracts of ln	18
been shown to be	18
of type i interferon	18
the complete genome sequences	18
and attenuation of virulence	18
the new ica was	18
an ion channel protein	18
epidemic diarrhea viruses from	18
is the first report	18
in published maps and	18
of the genus alphacoronavirus	18
adaptation in vitro and	18
and serial propagation of	18
attenuation of virulence in	18
in spike protein genes	18
diarrhea virus in porcine	18
maps and institutional affiliations	18
of virulence in vivo	18
global porcine epidemic diarrhea	18
rna was detected in	18
isolation and serial propagation	18
of type i ifn	18
we found that pedv	18
there were no significant	18
h at room temperature	18
were similar to those	18
plates were incubated for	18
in the swine industry	18
with regard to jurisdictional	18
positive cells in the	18
jurisdictional claims in published	18
were considered statistically significant	18
s gene of pedv	18
was performed as described	18
from the united states	18
the host innate immune	18
were found to be	18
the specificity of the	18
published maps and institutional	18
the replication of pedv	18
and functional characterization of	18
identified in this study	18
virus porcine epidemic diarrhea	18
with growth adaptation in	18
regard to jurisdictional claims	18
the coronavirus spike protein	18
were washed with pbs	18
higher than that of	18
in cell cultures and	18
and incubated for h	18
for h at room	18
losses to the swine	18
mortality in suckling piglets	18
in the small intestines	18
it has been shown	18
results suggest that the	18
in the family coronaviridae	18
heterogeneity in spike protein	18
and partial characterization of	18
vero cells were inoculated	18
spike glycoprotein gene of	18
as a vehicle for	18
the pcr products were	18
remains neutral with regard	18
neutral with regard to	18
with transmissible gastroenteritis virus	18
as a cause of	18
comparison of porcine epidemic	17
cultures and partial characterization	17
infected vero e cells	17
analysis of the orf	17
from germany and the	17
in this study are	17
novel mammalian and avian	17
blocked with bovine serum	17
structural and functional characterization	17
seven novel mammalian and	17
could be detected in	17
the n protein of	17
and avian coronaviruses in	17
in vitro and attenuation	17
cell cultures and partial	17
cell attenuated porcine epidemic	17
vitro and attenuation of	17
and avian coronaviruses as	17
in porcine intestinal epithelial	17
analysis of the spike	17
sequences of the s	17
mammalian and avian coronaviruses	17
encodes an ion channel	17
cells in the small	17
supports bat coronaviruses as	17
pigs porcine epidemic diarrhea	17
were collected from the	17
the survival of pedv	17
partial characterization of the	17
is a class i	17
germany and the united	17
of gammacoronavirus and deltacoronavirus	17
diarrhoea virus in italy	17
in the pathogenesis of	17
the nucleotide sequences of	17
diarrhea of a new	17
was used for the	17
mutations in the spike	17
alphacoronavirus and betacoronavirus and	17
quantification of transmissible gastroenteritis	17
fecal samples were collected	17
class i virus fusion	17
by porcine epidemic diarrhoea	17
induction of neutralizing antibodies	17
coronavirus spike protein is	17
associated with porcine epidemic	17
cloning and further sequence	17
in significant economic losses	17
viruses from germany and	17
source of alphacoronavirus and	17
discovery of seven novel	17
virus in cell cultures	17
at rt for min	17
were no significant differences	17
avian coronaviruses as the	17
no significant differences in	17
for the detection and	17
in the vero cells	17
were orally inoculated with	17
onset of clinical signs	17
source of gammacoronavirus and	17
and s t proteins	17
cells were washed three	17
the aim of this	17
and quantification of transmissible	17
characterization of the isolate	17
diarrhea virus strain from	17
for the duration of	17
bat coronaviruses as the	17
in addition to the	17
gene source of gammacoronavirus	17
was kindly provided by	17
detection limit of the	17
vaccines for porcine epidemic	17
aim of this study	17
gene source of alphacoronavirus	17
to those of the	17
betacoronavirus and avian coronaviruses	17
with different concentrations of	17
analysis of the partial	17
significantly higher than the	17
of seven novel mammalian	17
epidemic diarrhea virus nucleocapsid	17
further sequence analysis of	17
propagation of porcine epidemic	17
in serum and colostrum	17
and betacoronavirus and avian	17
associated with growth adaptation	17
type i and type	17
authors read and approved	17
detection of transmissible gastroenteritis	17
the early stage of	17
these data indicate that	17
deltacoronavirus supports bat coronaviruses	17
and further sequence analysis	17
the development of a	16
high morbidity and mortality	16
the complete genome sequence	16
in swine porcine epidemic	16
a class i virus	16
the mesenteric lymph nodes	16
epidemic diarrhea virus isolate	16
pedv rna was detected	16
until the end of	16
epidemic diarrhea virus genome	16
vero cells and ipec	16
mutations in the s	16
in the genus deltacoronavirus	16
study was to determine	16
i virus fusion protein	16
of the new ica	16
with an intact s	16
the fusion core complex	16
of the order nidovirales	16
performed as described previously	16
cells were maintained in	16
seven open reading frames	16
infection of vero cells	16
the porcine aminopeptidase n	16
ion channel protein and	16
coronaviruses in the genus	16
as the reciprocal of	16
infectivity of icpc a	16
letter to the editor	16
as a source of	16
characterization of a variant	16
the s proteins of	16
as described in the	16
of porcine circovirus type	16
was supported by the	16
of three independent experiments	16
had no effect on	16
of antibodies against porcine	16
pedv classical attenuated vaccine	16
of diarrhoea caused by	16
clinical signs of ped	16
protein is a class	16
by the addition of	16
serial propagation of porcine	16
viral rna was extracted	16
antibodies were detected in	16
pedv strain pc a	16
the activation of the	16
pedv at an moi	16
was first identified in	16
diarrhea virus in cell	16
were obtained from the	16
of pedv s protein	16
functional characterization of the	16
end of the trial	16
of the n protein	16
for h at rt	16
have been identified in	16
characterization of the fusion	16
the absence of trypsin	16
is a highly contagious	16
of the fusion core	16
in this study was	16
small and large intestines	16
when compared to the	16
of type i ifns	15
has been reported that	15
genomic rna and sg	15
more than of the	15
type i and iii	15
all authors read and	15
an equal volume of	15
of transportation in spread	15
transportation in spread of	15
diarrhea virus infection of	15
closely related to the	15
in spread of porcine	15
characterization of a novel	15
the transmissible gastroenteritis virus	15
epidemic diarrhea virus the	15
the results of this	15
in china isolation and	15
has been reported in	15
channel protein and regulates	15
min at rt and	15
avian coronaviruses in the	15
diarrhea viruses from germany	15
the results indicated that	15
role of transportation in	15
density to porcine epidemic	15
of the viral genome	15
pedv orf encodes an	15
were washed twice with	15
the genus deltacoronavirus supports	15
epidemic of diarrhoea caused	15
and then incubated with	15
most closely related to	15
that pedv n protein	15
diarrhoea caused by porcine	15
there was no significant	15
was performed using the	15
with pedv at a	15
have been shown to	15
and regulates virus production	15
receptor density to porcine	15
in the regulation of	15
the reciprocal of the	15
of the partial spike	15
to the genus alphacoronavirus	15
of sows with a	15
assay for the detection	15
were collected at hpi	15
to the pig industry	15
protein and regulates virus	15
duration of the study	15
infected or infected with	15
vero cell attenuated porcine	15
with pedv at an	15
are summarized in table	15
vero cells were treated	15
the culture medium was	15
previous studies have shown	15
may be due to	15
it has been demonstrated	15
for h prior to	15
membrane protein genes of	15
in the us and	15
genus deltacoronavirus supports bat	15
diarrhea virus and transmissible	15
in the lamina propria	15
has been reported to	15
in the spike protein	15
contribution of the porcine	15
paraformaldehyde for min at	15
of the porcine aminopeptidase	15
diarrhea virus field strains	15
and porcine group a	15
orf encodes an ion	15
the inhibitory effects of	15
investigation of porcine epidemic	15
was propagated in vero	15
h and then infected	15
have been reported in	15
in the orf a	15
fecal viral rna shedding	15
epidemic diarrhea virus variant	15
was obtained from the	14
nuclei were stained with	14
contaminated feed as a	14
diarrhea virus associated with	14
partial spike glycoprotein gene	14
incubated at room temperature	14
investigation into the role	14
the basis of the	14
epidemic diarrhea virus associated	14
followed by cycles of	14
the swine industry in	14
in a variety of	14
infected cells per well	14
at weeks of age	14
under a fluorescence microscope	14
the s protein was	14
recombinant s domain of	14
efficacy of vero cell	14
pedv n protein was	14
were inoculated orally with	14
the partial spike glycoprotein	14
diarrhea virus in southern	14
the nucleotide sequence of	14
diarrhea virus dr strain	14
of the virus to	14
there was no difference	14
the role of potentially	14
would like to thank	14
has been demonstrated that	14
did not show any	14
states porcine epidemic diarrhea	14
genotype b porcine epidemic	14
diarrhea in swine porcine	14
diarrhea and or vomiting	14
declare no conflict of	14
the institutional animal care	14
of porcine enteric coronaviruses	14
is a receptor for	14
particles associated with porcine	14
in the small and	14
s and s t	14
was removed and the	14
have been reported to	14
the late stage of	14
of the s domain	14
on porcine epidemic diarrhoea	14
potentially contaminated feed as	14
of vero cell attenuated	14
immunogenicity and protective efficacy	14
porcine epidemic diarrhoea in	14
into the role of	14
pedv n protein and	14
interaction between irf and	14
virus in southern germany	14
a source of the	14
and porcine epidemic diarrhoea	14
recombinant porcine epidemic diarrhea	14
are shown in table	14
studies are needed to	14
diarrhea virus and its	14
glycoprotein gene of porcine	14
were tested for the	14
the sialic acid binding	14
b porcine epidemic diarrhea	14
was no significant difference	14
coronavirus mouse hepatitis virus	14
aminopeptidase n is not	14
sensitivity and specificity of	14
field isolates in korea	14
diarrhea virus porcine epidemic	14
in the gastrointestinal tract	14
rapid detection of porcine	14
at hpi and hpi	14
with the n protein	14
oral efficacy of vero	14
region of the s	14
china isolation and characterization	14
daily for clinical signs	14
a large number of	14
cells were subjected to	14
the virulence of pedv	14
large deletions in the	14
vero cells infected with	14
can be used to	14
cells were fixed and	14
was extracted from the	14
the deduced amino acid	14
feed as a source	14
for rapid detection of	14
virus in serum and	14
has been shown that	14
cells were stained with	14
terminal region of the	14
was used to detect	14
rna and sg mrna	14
virus associated with growth	14
and infected with pedv	14
of viral rna in	14
of the reemerging korean	14
role of potentially contaminated	14
to prevent and control	14
the cell lysates were	14
were found in the	14
positive and negative controls	14
of potentially contaminated feed	14
room temperature for min	13
cells were collected at	13
i interferon production by	13
in europe and asia	13
deletion in the s	13
are shown in fig	13
s and n genes	13
positive ec cells in	13
of clinical signs and	13
authors would like to	13
pedv s protein was	13
in intestinal epithelial cells	13
between irf and tbk	13
of the pedv strain	13
cells in the presence	13
of the pedv spike	13
was approved by the	13
authors declare no conflict	13
strains with an intact	13
it is important to	13
the molecular biology of	13
a critical role in	13
efficacy of recombinant s	13
of the s and	13
was significantly lower than	13
t cells were co	13
in mesenteric lymph nodes	13
on ice for min	13
these results suggested that	13
cellular receptor for pedv	13
sequence and phylogenetic analysis	13
caused huge economic losses	13
in the us in	13
virus prototype and s	13
is worth noting that	13
i and type iii	13
cells were blocked with	13
a key role in	13
belongs to the genus	13
in the healthy groups	13
green monkey kidney cells	13
in the positive control	13
epidemic diarrhea virus into	13
between the united states	13
the invasion rates of	13
the pedv s gene	13
detection of pedv in	13
the authors would like	13
of the severe acute	13
identification and characterization of	13
was determined to be	13
was performed using a	13
in the control group	13
the vero cells were	13
amino acid sequence identity	13
blood samples were collected	13
vero cells were cultured	13
amino acid sequence of	13
epidemic diarrhea virus is	13
was similar to that	13
a role in the	13
and protective efficacy of	13
cells as described previously	13
between porcine epidemic diarrhea	13
cell entry of porcine	13
isolate of porcine epidemic	13
to the lack of	13
cells were washed twice	13
the first report of	13
using graphpad prism software	13
the infectivity of icpc	13
in the p group	13
in the feces of	13
the expression level of	13
with hematoxylin and eosin	13
pp a and pp	13
of a novel porcine	13
high mortality in neonatal	13
variants with high pathogenicity	13
pedv s protein is	13
transcription polymerase chain reaction	13
the interaction between irf	13
protein of the porcine	13
significant economic losses in	13
the spread of the	13
times with pbs and	13
genome sequences of the	13
agent of porcine epidemic	13
and porcine reproductive and	13
compared to that of	13
were performed using the	13
susceptible to pedv infection	13
it is known that	13
spread of the virus	13
deletions in the s	13
epidemic diarrhea virus isolates	13
the inhibitory effect of	13
on the s protein	13
clarified by centrifugation at	13
pigs inoculated orally with	13
and genetic characterization of	13
phylogenetic trees were constructed	13
pedv is an enveloped	13
as well as in	13
of apn positive cells	13
it is worth noting	13
serum samples were collected	13
for an additional h	13
virus variants with high	13
detected outbreaks of porcine	13
in the case of	13
of recombinant s domain	13
s protein of the	13
were added to the	13
in hek t cells	13
other pedv reference strains	13
from each group were	13
via natural feeding behavior	13
coe and rlppg f	13
but not in the	13
losses in the swine	13
suppression of type i	13
is a type i	13
in the mesenteric lymph	13
pedv infection in vero	13
the crude extract containing	13
source of the first	13
the introduction of pedv	13
alphacoronavirus in the family	12
deletion porcine epidemic diarrhea	12
diarrhea virus variants with	12
elisa for the detection	12
the limit of detection	12
reverse transcription polymerase chain	12
this article can be	12
of the pedv n	12
cells were maintained at	12
evaluation of contaminated complete	12
were observed between the	12
virus and discovery of	12
pigs following consumption via	12
epidemic diarrhea in china	12
analysis of membrane protein	12
a herd of breeding	12
a coronavirus related to	12
diarrhea virus nucleocapsid protein	12
for min at c	12
for pedv and tgev	12
to each well and	12
piglets born to immunized	12
herd of breeding and	12
a cause of persistent	12
terminal domain of the	12
related to human coronavirus	12
in vero cells as	12
rectal swabs were collected	12
are available from the	12
rna was extracted using	12
in the development of	12
as well as to	12
phylogenetic analysis of membrane	12
complete feed as a	12
diarrhea virus and discovery	12
pedv s and s	12
the mrna levels of	12
virus as a cause	12
following consumption via natural	12
rna genome of approximately	12
of membrane protein genes	12
virus isolates in china	12
and colostrum by indirect	12
by western blot analysis	12
the sequence of the	12
virus and porcine respiratory	12
of the pedv strains	12
infected with pedv strains	12
the culture supernatants were	12
the orf a b	12
washed twice with pbs	12
for h followed by	12
were randomly divided into	12
of a recombinant swine	12
were expressed as the	12
in the orf gene	12
type porcine epidemic diarrhea	12
virulent strains of pedv	12
coronavirus porcine epidemic diarrhea	12
feed as a vehicle	12
replaced with fresh dmem	12
diarrhea virus infection in	12
than that in the	12
consumption via natural feeding	12
numbers of pedv antigen	12
a novel swine enteric	12
plays a critical role	12
of contaminated complete feed	12
significantly higher than those	12
a vehicle for porcine	12
orf gene of wild	12
s and s domains	12
pbs for min at	12
vehicle for porcine epidemic	12
m and n genes	12
evolutionary genetics analysis version	12
passages in vero cells	12
in any of the	12
colostrum by indirect elisa	12
article can be found	12
and pathogenicity of global	12
these data suggest that	12
and the role of	12
as well as a	12
cells with bound tgev	12
the reemerging korean pedv	12
antigenicity and pathogenicity of	12
cd t cell frequency	12
contaminated complete feed as	12
and discovery of a	12
in the united kingdom	12
end of the study	12
diarrhea virus isolates in	12
virus and porcine deltacoronavirus	12
high sensitivity and specificity	12
in the infected piglets	12
in pbs for min	12
the course of the	12
g a pedv strain	12
were obtained from a	12
vero cells in the	12
in agreement with the	12
with pedv strains at	12
were inoculated with the	12
epidemic diarrhea virus variants	12
was shown to be	12
were approved by the	12
results indicate that the	12
in the s and	12
the present study was	12
in vero cells in	12
been reported to be	12
for h at c	12
the united kingdom in	12
crude extract containing coe	12
losses to the pig	12
in the context of	12
have shown that the	12
a recombinant swine enteric	12
recombinant swine enteric coronavirus	12
variant with a large	12
in porcine epidemic diarrhea	12
classical swine fever virus	12
at h after transfection	12
diarrhea virus in serum	12
the viral spike protein	12
amino acid sequences were	12
serum and colostrum by	12
the type i interferon	12
for min and then	12
in a herd of	12
discovery of a recombinant	12
pathogenicity of global porcine	12
sequence of a novel	12
acid sequences of the	12
the nucleocapsid protein gene	12
of the gds and	12
american type culture collection	12
which belongs to the	12
were then incubated with	12
natural science foundation of	12
was used to determine	12
late stage of infection	12
was calculated using the	12
protective efficacy of recombinant	12
the care and use	12
with the intracellular extracts	12
for detection of pedv	12
tested for the presence	12
insertion deletion porcine epidemic	12
an evaluation of contaminated	12
identification of two novel	12
a high mortality rate	12
virulent porcine epidemic diarrhea	12
the number of pedv	12
two novel b cell	11
cause of persistent diarrhoea	11
for up to days	11
supported by a grant	11
epidemic diarrhea virus a	11
nucleotide sequence of the	11
of experimental cv coronavirus	11
an acute and highly	11
proteolytic activation of the	11
epitopes on porcine epidemic	11
on the s gene	11
of a novel recombinant	11
divided into three groups	11
antigenic relationships among porcine	11
of the virus and	11
of pedv and tgev	11
transmissible gastroenteritis virus strains	11
usage and cell entry	11
the integrity of the	11
is associated with the	11
of breeding and finishing	11
were located in the	11
all samples were tested	11
centrifuged for min at	11
genome of approximately kb	11
as a receptor for	11
a and g b	11
porcine enteric coronaviruses and	11
for the care and	11
committee on taxonomy of	11
the inoculum was removed	11
is involved in the	11
cell epitopes on porcine	11
breeding and finishing pigs	11
biological and physicochemical properties	11
the s and n	11
b cell epitopes on	11
of persistent diarrhoea in	11
the mucosal immune system	11
nucleocapsid genes of porcine	11
and porcine transmissible gastroenteritis	11
bmc vet res doi	11
statistical analyses were performed	11
coronaviridae of the order	11
were carried out in	11
human immunodeficiency virus type	11
virus nucleocapsid protein antagonizes	11
weeks prior to farrowing	11
human coronavirus e and	11
clinical signs of diarrhea	11
a gnotobiotic pig model	11
viral rna in serum	11
height to crypt depth	11
after washing three times	11
cd t cell frequencies	11
can be used for	11
be used as a	11
an immunohistochemical investigation of	11
international committee on taxonomy	11
vero cells treated with	11
and analysis of a	11
of the negative control	11
strain isolated in france	11
the age of the	11
the invasion efficiency of	11
novel b cell epitopes	11
villous epithelial cells of	11
mock infected or infected	11
sequence of the pedv	11
based on the orf	11
analysis of the complete	11
sequestering the interaction between	11
the complete genome of	11
attenuated strains of porcine	11
the spread of pedv	11
the m protein of	11
western blot analysis of	11
the number of new	11
of the innate immune	11
receptor usage and cell	11
of the family coronaviridae	11
passage in vero cells	11
way analysis of variance	11
sows with a mild	11
at pid compared to	11
relationships among porcine epidemic	11
phylogenetic trees based on	11
t cells were transfected	11
epidemic diarrhea virus outbreaks	11
weaned pigs at pid	11
classical attenuated vaccine strain	11
experimental cv coronavirus enteritis	11
coronavirus enteritis in piglets	11
epidemic diarrhea s gene	11
i and iii ifns	11
in the infected groups	11
the cells were infected	11
persistent diarrhoea in a	11
ec cells in the	11
production by sequestering the	11
genome sequencing and analysis	11
a novel outbreak in	11
study was funded by	11
of two novel b	11
cv coronavirus enteritis in	11
in contrast to the	11
pigs had significantly higher	11
protein antagonizes beta interferon	11
harbin veterinary research institute	11
at a dilution of	11
of the ica kit	11
porcine epidemic diarrhea s	11
to a final concentration	11
incubated for min at	11
ch jx and cv	11
causative agent of porcine	11
the presence of antibodies	11
pedv n protein is	11
in mice group g	11
cd cd t cells	11
and physicochemical properties of	11
on taxonomy of viruses	11
slips through us borders	11
nucleocapsid protein gene of	11
derivation of attenuated porcine	11
against the s protein	11
indel strain isolated in	11
the complete s gene	11
might be due to	11
pedv cv and lnct	11
of pedv infection in	11
in china complete genome	11
in cells treated with	11
diarrhea virus outbreaks in	11
in the production of	11
have been used to	11
detection of pedv antigen	11
virus outbreaks in taiwan	11
analysis showed that the	11
suckling pigs compared to	11
further studies are needed	11
of the present study	11
cells in the g	11
infection of sows with	11
among porcine epidemic diarrhea	11
s and s subunits	11
is based on the	11
version of this article	11
novel outbreak in belgium	11
deadly pig virus slips	11
diarrhea s gene indel	11
virus strain from henan	11
s gene indel strain	11
the vero and ipec	11
villous atrophy in the	11
phylogenetic tree based on	11
were fixed with formaldehyde	11
the family coronaviridae of	11
pathology of experimental cv	11
high mortality rates in	11
pig virus slips through	11
transmissible gastroenteritis virus infection	11
family coronaviridae of the	11
the percentage of apn	11
pcr was performed using	11
colonic mucosa and content	11
in the sera of	11
due to the lack	11
strains prevailing recently in	11
with large deletions in	11
the detection limits of	11
cloning and sequence analysis	11
a high level of	11
antibody responses in serum	11
virus field strains prevailing	11
length genome sequence of	11
by sequestering the interaction	11
and cell entry of	11
cells were harvested and	11
sequencing and analysis of	11
mouse proximal apn promoter	11
resulting in significant economic	11
villous and crypt epithelium	11
compared to weaned pigs	11
aminopeptidase n and its	11
virus in south china	11
the vero e cells	11
stained with hematoxylin and	11
of spike gene of	11
against the pedv s	11
was funded by the	11
is the most abundant	11
we were able to	11
immunohistochemical investigation of porcine	11
virus slips through us	11
indel strain of porcine	11
diarrhoea in a herd	11
interferon production by sequestering	11
may be involved in	11
previous infection of sows	11
with the results of	11
prevention and control of	11
pigs infected with porcine	11
were blocked with bovine	11
supernatants were collected at	11
gene indel strain isolated	11
mice orally immunized with	11
against pedv in vero	11
rapid differential detection of	11
compared to those of	11
prevailing recently in china	11
medium was replaced with	11
confirmed the presence of	11
inoculated orally with pedv	11
in a humidified co	11
the content of the	11
were inoculated with pedv	11
field strains prevailing recently	11
coronavirus infectious bronchitis virus	11
of nucleocapsid genes of	11
van cott et al	11
were randomly assigned to	11
diarrhea in piglets in	10
diarrhoea virus as a	10
severity of clinical signs	10
and protection in conventional	10
reciprocal of the highest	10
for porcine epidemic diarrhoea	10
antagonizes beta interferon production	10
our data indicate that	10
of the authors of	10
of type i and	10
elisa using monoclonal antibodies	10
in the s region	10
green monkey kidney cell	10
novel swine enteric alphacoronavirus	10
and ileum are the	10
financial or personal relationship	10
was defined as the	10
analysis of a novel	10
bias the content of	10
samples were tested in	10
plasma is not infectious	10
mediated by the viral	10
infected suckling pigs had	10
the small intestines of	10
virulent or attenuated porcine	10
or infected with pedv	10
dna and rna viruses	10
europe and matters of	10
of a coronavirus spike	10
insertions and deletions in	10
epidemiology and vaccine of	10
of vero cells with	10
is essential for induction	10
the surface of the	10
vaccine of porcine epidemic	10
in vero cells and	10
deposited in genbank under	10
neutralizing antibodies in the	10
pedv at a multiplicity	10
in the phylogenetic tree	10
higher than that in	10
physicochemical properties of cell	10
the cellular receptor for	10
in the genbank database	10
the creative commons attribution	10
the role of apn	10
personal relationship with other	10
to be positive for	10
national natural science foundation	10
thirds of the genome	10
in the healthy piglets	10
pcr for the detection	10
washed with pbs and	10
beta interferon production by	10
has been used to	10
presence of pedv rna	10
is not infectious to	10
orally with ml of	10
in a previous study	10
essential for induction of	10
was replaced with fresh	10
of cells in the	10
in the large intestine	10
the s protein in	10
is a major receptor	10
from europe and matters	10
of icpc a and	10
pedv and tgev infection	10
or bias the content	10
people or organizations that	10
of antibodies against pedv	10
and mesenteric lymph nodes	10
porcine cell cultures and	10
the majority of the	10
porcine plasma is not	10
were treated with the	10
organizations that could inappropriately	10
a mild strain of	10
a high degree of	10
in response to pedv	10
into s and s	10
s protein is a	10
monoclonal antibodies for the	10
molecular characterization of the	10
was first detected in	10
used to amplify the	10
three times with pbst	10
for clinical signs of	10
could inappropriately influence or	10
other people or organizations	10
and in the vero	10
is essential for the	10
was cloned into the	10
at the day of	10
tissue culture infective dose	10
the in vitro prophylactic	10
or personal relationship with	10
n is a major	10
and immunogenicity of recombinant	10
innate immune responses to	10
suckling pigs at pid	10
and vaccine of porcine	10
nucleocapsid protein antagonizes beta	10
and matters of debate	10
piglets infected with pedv	10
and in situ hybridization	10
the university of minnesota	10
vero cells as described	10
the presence of a	10
be responsible for the	10
were performed in triplicate	10
authors of this paper	10
outbreaks in south korea	10
of the pedv genome	10
in the pig industry	10
each well and incubated	10
passages in cell culture	10
rna virus belonging to	10
chinese porcine epidemic diarrhea	10
than those in the	10
detection and molecular diversity	10
particles associated with diarrhea	10
a vital role in	10
the presence of ribavirin	10
and in the ipec	10
diarrhea virus variant with	10
analyses based on the	10
cell cultures and experimental	10
were significantly lower than	10
washing three times with	10
or organizations that could	10
novel recombinant porcine epidemic	10
molecular biology of coronaviruses	10
by a grant from	10
cycles of denaturation at	10
virus and its antibodies	10
and incubated for min	10
spike protein of the	10
and fecal samples were	10
analyses were performed using	10
the development of effective	10
virus rna present in	10
pedv spike protein gene	10
the remainder of the	10
antibodies for the detection	10
the authors of this	10
protection against infection with	10
igg and iga antibodies	10
were monitored daily for	10
major receptor for the	10
monkey kidney cell line	10
analyzed by western blotting	10
at the beginning of	10
results showed that pedv	10
responses and protection in	10
a retrospect from europe	10
vero cells were pretreated	10
plays a role in	10
fecal pedv rna shedding	10
gene of the porcine	10
the global swine industry	10
diversity of spike gene	10
of peptide h on	10
pigs orally inoculated with	10
related to transmissible gastroenteritis	10
to those in the	10
inappropriately influence or bias	10
at the late stage	10
using monoclonal antibodies for	10
care and use of	10
rna present in commercial	10
south dakota state university	10
were challenged orally with	10
from a novel outbreak	10
between fcov type and	10
a financial or personal	10
detection and differentiation of	10
jejunum and ileum are	10
properties of cell culture	10
villous height to crypt	10
was present in the	10
in the intestine of	10
detection and genetic characterization	10
vero cells grown in	10
depletion of cellular cholesterol	10
samples were collected at	10
the presence of m	10
in each group were	10
coronavirus transmissible gastroenteritis virus	10
were purchased from sigma	10
of apn negative cells	10
serum samples collected from	10
relationship with other people	10
as a viral deubiquitinase	10
the s protein and	10
s gene of the	10
and experimental infection of	10
the international committee on	10
venezuelan equine encephalitis virus	10
a novel recombinant porcine	10
the first line of	10
cultures and experimental infection	10
the entire genome of	10
that could inappropriately influence	10
with the exception of	10
retrospect from europe and	10
portion of the s	10
a major receptor for	10
of the pedv outbreak	10
to a nitrocellulose membrane	10
present in commercial spray	10
positive for pedv by	10
binding domain complexed with	10
fusion of the viral	10
in colostrum and milk	10
in a gnotobiotic pig	10
enteric disease in pigs	10
sense rna genome of	10
and molecular characterization of	10
s proteins on the	10
and crypt epithelium of	10
objective of this study	10
plates were washed three	10
pigs of different ages	10
against infection with a	10
pathogenesis of porcine epidemic	10
the early stages of	10
entry into host cells	10
have been identified as	10
by the institutional animal	10
of the sars coronavirus	10
at a density of	10
the role of the	10
in the negative control	10
at days of age	10
to the family coronaviridae	10
molecular diversity of spike	10
is related to the	10
virus in porcine cell	10
in porcine alveolar macrophages	10
the amino acid sequences	10
huge economic losses to	10
bile and bile acids	10
in vitro prophylactic effects	10
influence or bias the	10
protein of pedv is	10
is not required for	10
to further investigate the	10
and deletions in the	10
of the ohio state	10
specific siga positive colostrum	10
balb c mice were	10
or attenuated porcine epidemic	10
jnk and erk phosphorylation	10
with other people or	10
pigs infected with pedv	10
epidemic diarrhea virus identification	10
to human coronavirus e	10
n protein of pedv	10
data are shown as	10
was no difference in	10
the infected weaned pigs	10
epidemic diarrhoea virus as	10
were cloned into the	10
infection of pigs of	10
with a mild strain	10
rates of the gds	10
the g g phase	10
the cd cd t	10
in porcine cell cultures	10
dried porcine plasma is	10
and molecular diversity of	10
were inoculated with kpedv	10
this study was funded	10
in the infected group	10
in piglets born to	10
divided into four groups	10
swine porcine epidemic diarrhoea	10
diarrhea virus and porcine	10
activation of the porcine	9
crude extract containing trimeric	9
transmissible gastroenteritis and porcine	9
colorado from the united	9
infection in vero e	9
currently circulating in the	9
and feline infectious peritonitis	9
genetic characterization of porcine	9
immunodominant region of s	9
subjected to virus isolation	9
open access article distributed	9
rotavirus in a gnotobiotic	9
response to pedv infection	9
fusion protein by trypsin	9
is not a cellular	9
with the pedv s	9
epidemic diarrhea virus rna	9
the results demonstrated that	9
intestinal and fecal samples	9
cells were overlaid with	9
was considered statistically significant	9
of the coronaviridae family	9
type i ifn response	9
determination of the nucleocapsid	9
pedv in the presence	9
compared to the control	9
coronavirus spike fusion protein	9
analysis of the porcine	9
the orf gene and	9
in vero cells at	9
were calculated using the	9
genome sequence of pedv	9
and its inhibition by	9
the original us pedv	9
the amount of pedv	9
and molecular epidemiology of	9
antigenic relationship between the	9
antibodies in serum samples	9
causative agent of ped	9
causes endoplasmic reticulum stress	9
of n protein with	9
could be used to	9
of the creative commons	9
to the best of	9
cells were incubated for	9
are expressed as mean	9
e and porcine transmissible	9
of q r on	9
and rapid detection of	9
was supported by a	9
was carried out using	9
epidemic diarrhea coronavirus spike	9
to the order nidovirales	9
heterogeneous nuclear ribonucleoprotein a	9
the pedv strain cv	9
a large amount of	9
at room temperature and	9
of pedv n and	9
an original us porcine	9
a significant increase in	9
our data indicated that	9
indel and pedv s	9
detection of viral rna	9
than those induced by	9
antiviral innate immune response	9
end of the experiment	9
were positive for pdcov	9
the best of our	9
protective immunity to human	9
at h after the	9
characterization of pig epidemic	9
not a cellular receptor	9
protein is the most	9
was added and incubated	9
evidence of infectivity of	9
pedv infection in the	9
in viral entry and	9
strain usa colorado from	9
the differentially expressed genes	9
new insights into the	9
original us porcine epidemic	9
was to determine the	9
swine porcine epidemic diarrhea	9
viral rna at long	9
the s gene and	9
at rt and then	9
p e d v	9
analysis of the s	9
attenuated vaccine strain cv	9
the pathogenesis of pedv	9
virus from a novel	9
with an equal volume	9
has a financial or	9
were used to amplify	9
in gilts and sows	9
may play an important	9
presence of pedv in	9
pedv in the field	9
well and incubated at	9
respiratory syndrome virus nucleocapsid	9
our understanding of the	9
characterized by watery diarrhea	9
fold serial dilutions of	9
that the s protein	9
detection of airborne viral	9
the mouse proximal apn	9
for rapid differential detection	9
severe diarrhea and vomiting	9
in the early stage	9
infectivity of airborne porcine	9
at g for min	9
and viral modulation of	9
immune evasion of porcine	9
molecular characterization of porcine	9
the highest serum dilution	9
the antigenic relationship between	9
of pedv have been	9
at a final concentration	9
proteins have been identified	9
be divided into two	9
results are expressed as	9
number of new cases	9
the detection of the	9
in the intestines of	9
pedv as well as	9
incubated for an additional	9
tcid ml of pedv	9
were then washed with	9
the severity of clinical	9
by the viral spike	9
a simple method of	9
a and g a	9
and downstream mediators on	9
the national natural science	9
note springer nature remains	9
the levels of pedv	9
terminus of the s	9
protein gene of the	9
high mortality in suckling	9
orally immunized with rlppg	9
strains at h after	9
and cloned into the	9
could provide piglets against	9
vero cells grown on	9
diarrhea virus strain usa	9
phylogenetic analysis of nucleocapsid	9
diarrhea virus confers protection	9
paper has a financial	9
prrsv and pedv were	9
severe watery diarrhea and	9
and evolution of the	9
is an open access	9
of a new porcine	9
cleavage of the s	9
pigs exposed to virulent	9
reemerging porcine epidemic diarrhea	9
based on the nucleotide	9
attenuated vaccine strains and	9
groups a and b	9
with acute diarrhea in	9
of pedv antigen in	9
high mortality in piglets	9
for induction of neutralizing	9
virus and detection of	9
provide piglets against homologous	9
this s indirect elisa	9
pcr and virus isolation	9
diarrhea virus in swine	9
an open access article	9
of acute pedv infection	9
entry mediated by the	9
in the number of	9
the detection of antibodies	9
of the expression of	9
attenuated porcine epidemic diarrhoea	9
analysis of nucleocapsid genes	9
spike fusion protein by	9
and the presence of	9
trypsin in cell culture	9
antiviral activity of ribavirin	9
modulation of antiviral innate	9
of coronavirus cell entry	9
of infectivity of airborne	9
long distances from infected	9
samples were subjected to	9
our results suggest that	9
virus infection of naive	9
virus porcine epidemic diarrhoea	9
diarrhea virus in portugal	9
and gds strains were	9
of pedv and other	9
epidemic diarrhea virus replication	9
based on the complete	9
molecular characterization of pig	9
replicates in porcine alveolar	9
the random forest model	9
were used in this	9
were determined to be	9
of pigs of different	9
mild strain of porcine	9
distributed under the terms	9
results indicated that pedv	9
in the replication of	9
be involved in the	9
development and evaluation of	9
antibodies were purchased from	9
the data indicated that	9
rna at long distances	9
the primary sites of	9
of the interaction between	9
with other pedv strains	9
a and pp ab	9
confers protection against infection	9
the function of the	9
airborne viral rna at	9
was first discovered in	9
diarrhea virus rna present	9
for samples stored at	9
shedding was detected in	9
on the antigenic relationship	9
cells were then washed	9
mechanisms of coronavirus cell	9
transmissible gastroenteritis virus in	9
at the indicated times	9
simple method of estimating	9
transferred to a nitrocellulose	9
were deposited in the	9
was determined by a	9
evasion of porcine enteric	9
invasion efficiency of pedv	9
immune responses induced by	9
best of our knowledge	9
the pedv genome is	9
the cbp degradation by	9
and detection of airborne	9
extract containing trimeric coe	9
were collected from piglets	9
positive for pedv rna	9
at h post infection	9
membranes were incubated with	9
of this paper has	9
in the online version	9
of a novel swine	9
in vero and ipec	9
hybridization for the detection	9
diarrhea virus from a	9
enteric coronaviruses and viral	9
tge ped detection kit	9
piglets against homologous challenge	9
was visualized using a	9
infected pigs at pids	9
on the viral surface	9
the production of type	9
epidemic diarrhea virus shedding	9
exposed to virulent or	9
to determine whether pedv	9
observed in this study	9
the genetic diversity of	9
the porcine apn transgene	9
crude extract against pedv	9
herpes simplex virus type	9
n protein prolongs s	9
trees based on the	9
by the ministry of	9
exhibited lower antigenic indexes	9
the infected cells were	9
respiratory syndrome coronavirus spike	9
the first case of	9
may contribute to the	9
infected with pedv non	9
fcov type specific sera	9
moi of for h	9
were found to have	9
epidemic diarrhea virus confers	9
of middle east respiratory	9
diarrhea coronavirus spike fusion	9
differential detection of transmissible	9
isolated from a suckling	9
of the most important	9
of an original us	9
the attachment and entry	9
alpha minimum essential medium	9
cov pl pro s	9
virus is essential for	9
pedv rna in the	9
distances from infected herds	9
is the causative agent	9
science foundation of china	9
coronaviruses and viral modulation	9
isolated in japan from	9
for the purpose of	9
virus confers protection against	9
investigate the effect of	9
virus strain usa colorado	9
method of estimating fifty	9
without blfp crude extract	9
in situ hybridization for	9
of the assay was	9
infection of naive pigs	9
ped was first reported	9
induces endoplasmic reticulum stress	9
infection with a severe	9
cells were incubated at	9
of the partial s	9
g b and g	9
in europe in the	9
porcine hemagglutinating encephalomyelitis virus	9
viruses isolated in japan	9
access article distributed under	9
recombinant s protein was	9
of airborne viral rna	9
relationship between the coronavirus	9
in a humidified incubator	9
with porcine reproductive and	9
proteolytic cleavage of the	9
and deduced amino acid	9
orally inoculated with the	9
usa colorado from the	9
by trypsin in cell	9
syndrome virus nucleocapsid protein	9
production of type i	9
cell entry mediated by	9
percentage of sows mated	9
culture supernatants were collected	9
the cells were blocked	9
mouse hepatitis virus strain	9
pedv icpc a and	9
this paper has a	9
conventional pigs exposed to	9
protein by trypsin in	9
virulent strain of pedv	9
j cells were transfected	9
n is a receptor	9
from a suckling piglet	9
of airborne porcine epidemic	9
inoculum was removed and	9
the virulence of the	9
our data showed that	9
virus belonging to the	9
based on the sequence	9
signs of diarrhea and	9
diarrhea virus and the	9
positive ec cells were	9
in conventional pigs exposed	9
phylogenetic tree was constructed	9
coronavirus e and porcine	9
values are expressed as	9
plates were infected with	9
data are expressed as	9
coronavirus cell entry mediated	9
reemerging korean pedv strains	9
as described previously with	9
fecal and serum samples	9
viral modulation of antiviral	9
diarrhea virus into vero	9
subtypes g and g	9
diarrhea virus is essential	9
of pedv icpc a	9
assays for the detection	9
in the serum of	9
of the us strains	9
blfp crude extract against	9
study on the antigenic	9
article distributed under the	9
shown in figure b	9
were consistent with the	9
total number of pigs	9
differential detection of porcine	9
pigs experimentally infected with	9
in the s domain	9
were washed once with	9
m and orf genes	9
mice vaccinated with the	9
discovery of a novel	9
nucleocapsid and spike protein	9
serial passages in vero	9
losses to the global	9
clinical signs of pedv	9
of antiviral innate signaling	9
epidemic diarrhea virus subtypes	9
at long distances from	9
the role of viral	9
and rnase treated vaccine	9
cellular receptor of porcine	9
of mice vaccinated with	9
significant differences between the	9
vero cells with the	9
the rapid spread of	9
pedv at a moi	8
of an enteric infection	8
these results revealed that	8
provided by state and	8
followed by incubation with	8
respiratory syndrome coronavirus infection	8
not infectious to naive	8
of pedv in vitro	8
may be related to	8
pig epidemic diarrhoea viruses	8
viral entry and pathogenesis	8
of the dna damage	8
isolated from kefir grains	8
pedv was first reported	8
at the indicated time	8
incubated with mouse anti	8
after the onset of	8
gastroenteritis virus infection in	8
the yc pedv strain	8
research was supported by	8
cells of the small	8
cell frequencies in blood	8
cats and chinese ferret	8
in gnotobiotic pigs porcine	8
of the transmissible gastroenteritis	8
were subjected to three	8
comparison between the united	8
an enteric infection in	8
in pbs at rt	8
pedv was detected in	8
in this study we	8
an elisa for the	8
deletion in the orf	8
test for the detection	8
support were provided by	8
was chosen as the	8
sequence of the spike	8
tree based on the	8
immunofluorescent study on the	8
reveals a polymorphic orf	8
is characterized by vomiting	8
a devastating enteric disease	8
were incubated with pedv	8
might be associated with	8
in the supernatants of	8
in the beijing area	8
constructed from the aligned	8
genetic characterization of deltacoronavirus	8
pigs at pids and	8
coronavirus related to human	8
this virus is a	8
from porcine epidemic diarrhea	8
twice with pbs and	8
of the intestinal epithelium	8
pedv strains in this	8
of an indirect elisa	8
epidemic diarrhoea viruses isolated	8
pcr assay for the	8
of deltacoronavirus in pigs	8
a humidified co incubator	8
are the primary sites	8
corresponding author on reasonable	8
sequence determination of the	8
flexible strategies for multiple	8
host innate immune responses	8
the highly virulent pedv	8
pathogenesis comparison between the	8
and severe acute respiratory	8
pigs inoculated with the	8
is important for the	8
partial s gene sequences	8
on vero cell monolayers	8
from mesenteric lymph nodes	8
treated with different concentrations	8
university institutional animal care	8
strains in this study	8
of the korean pedv	8
agricultural research and development	8
that the recombinant s	8
s indirect elisa was	8
the n protein is	8
studies have demonstrated that	8
associated with cell adaptation	8
strategies for multiple sequence	8
vaccines emergence of porcine	8
to be associated with	8
nucleocapsid protein of porcine	8
of coronavirus attachment to	8
of a liquid antimicrobial	8
impact on the swine	8
used to detect pedv	8
in piglets less than	8
and chinese ferret badgers	8
update on porcine epidemic	8
in the usa in	8
were subjected to sds	8
seems to be a	8
purchased from santa cruz	8
virulent parental attenuated strains	8
emerging and reemerging epizootic	8
and spike protein genes	8
to the cytoplasm and	8
in swine farms in	8
the terms and conditions	8
were washed times with	8
available from the corresponding	8
acting as a viral	8
for multiple sequence alignment	8
of the vero cells	8
virus n protein prolongs	8
t cell frequency in	8
diarrhea virus identification of	8
nucleocapsid protein of sars	8
research support were provided	8
to those of other	8
antibody responses and protection	8
of coronavirus spike proteins	8
was first described in	8
promotes its infectivity via	8
identified in the us	8
content of the paper	8
prevalence of porcine epidemic	8
united states and canada	8
approved by the institutional	8
classical and variant virulent	8
and the results are	8
in the icpc a	8
epidemiological status of pedv	8
inoculated orally with ml	8
production of neutralizing antibodies	8
were cultured in alpha	8
blocking elisa using monoclonal	8
plates were incubated at	8
protein genes reveals a	8
intestinal epithelial cell line	8
i interferon pathway by	8
effusion disease in rabbits	8
with large genomic deletion	8
different from those of	8
faculty of veterinary medicine	8
data are presented as	8
as well as other	8
these results demonstrated that	8
the course of infection	8
impacts of porcine epidemic	8
of porcine transmissible gastroenteritis	8
papn ectodomain and pedv	8
by acting as a	8
the infection of pedv	8
cells were permeabilized with	8
were rinsed with pbs	8
the nucleotide and amino	8
in south korea in	8
the mechanism by which	8
pigs in each group	8
severe diarrhea and or	8
the membrane was incubated	8
mean numbers of serotonin	8
viral protein nsp in	8
cells and and in	8
the s gene is	8
the coronavirus transmissible gastroenteritis	8
were plotted using imagej	8
to pedv infection and	8
the murine coronavirus spike	8
the pathogenesis of an	8
during the course of	8
your next manuscript to	8
prevailing in central china	8
analysis of porcine deltacoronavirus	8
diarrhoea virus and transmissible	8
and and in the	8
were extracted using the	8
infected weaned pigs had	8
strains in conventional neonatal	8
was measured at nm	8
probably due to the	8
and embedded in paraffin	8
findings of this study	8
circulating in the us	8
in the jejunum of	8
state and federal funds	8
based on the results	8
is a coronavirus related	8
to host aminopeptidase n	8
entry of the porcine	8
comparative genomic analysis of	8
of the murine coronavirus	8
supported by the national	8
spike genes of porcine	8
of classical and variant	8
on the expression of	8
aided by quality analysis	8
nk cell frequencies in	8
protection in conventional pigs	8
genes induced by ifn	8
cv vero adapted strain	8
characteristics of the spike	8
protein causes endoplasmic reticulum	8
to be free of	8
the phylogenetic tree based	8
and federal funds appropriated	8
emergence of pedv in	8
by quality analysis tools	8
interferon pathway by acting	8
epidemic diarrhea virus coronavirus	8
pbs at rt for	8
infects and replicates in	8
parental attenuated strains of	8
to the original us	8
the rest of the	8
this research was supported	8
to better understand the	8
microscope coverslips placed in	8
types of pedv strains	8
no conflicts of interest	8
a total volume of	8
virus and degradation of	8
throughout the small intestine	8
ohio department of agriculture	8
virus confirms that this	8
protein is responsible for	8
epidemic diarrhea virus c	8
the iowa state university	8
was not detected in	8
epizootic swine virus porcine	8
high mortality rate in	8
after three washes with	8
other cov n proteins	8
used to determine the	8
than that of cv	8
coronavirus attachment to host	8
and clarified by centrifugation	8
the infectivity of pedv	8
porcine epidemic diarrhoea porcine	8
infection with porcine epidemic	8
cholesterol is required for	8
enteric infection in pigs	8
these findings suggest that	8
into vero e cells	8
grown on microscope coverslips	8
reemerging epizootic swine virus	8
study are available from	8
pretreated vero cells with	8
against the n protein	8
showing a positive signal	8
submit your next manuscript	8
enteric disease characterized by	8
virus shedding in the	8
the prevalence of pedv	8
and reemerging epizootic swine	8
endoplasmic reticulum stress and	8
it is likely that	8
with other pedv reference	8
of korean pedv field	8
type i ifn signaling	8
and pedv n protein	8
in vero cells infected	8
virus genome using targeted	8
kit according to the	8
variants with large deletions	8
nsp in irf signaling	8
an emerging and reemerging	8
higher than those of	8
piglets infected with porcine	8
of two porcine epidemic	8
of piglets born to	8
its inhibition by neutralizing	8
under the terms and	8
with pedv in the	8
that there is a	8
the development of new	8
genome between the nucleocapsid	8
virus and the role	8
it has been suggested	8
and degradation of creb	8
genome structure and replication	8
are closely related to	8
kindly provided by dr	8
coronavirus spike proteins in	8
in the middle of	8
genomic analysis of classical	8
of pedv in swine	8
federal funds appropriated to	8
have contributed to the	8
alignment aided by quality	8
showed that pedv infection	8
article is an open	8
analysis of classical and	8
of the membrane protein	8
for prrsv and pedv	8
for h and infected	8
centrifuged at rpm for	8
and variant virulent parental	8
scale outbreak of diarrhea	8
diarrhea virus genome between	8
staining was visualized using	8
variant virulent parental attenuated	8
pcr assays for the	8
inhibition by neutralizing antibodies	8
infectious to naive pigs	8
to biomed central and	8
the nuclear translocation of	8
hepatitis virus strain a	8
role of viral protein	8
a crucial role in	8
and specificity of the	8
protein nsp in irf	8
with bound tgev purdue	8
proteins in viral entry	8
states porcine epidemic diarrhoea	8
interferon restriction by porcine	8
in the endoplasmic reticulum	8
diarrhea virus by duplex	8
cellular entry of the	8
survival of pedv in	8
evaluation of porcine epidemic	8
airborne porcine epidemic diarrhea	8
epidemic diarrhea virus on	8
virulent genotype b porcine	8
using targeted rna recombination	8
of the disease in	8
experimentally induced by the	8
a high number of	8
with porcine respiratory coronavirus	8
with pedv at moi	8
orf a b gene	8
porcine small intestine epithelial	8
were added to each	8
at the protein level	8
clinical signs were observed	8
the cells were harvested	8
the porcine reproductive and	8
stage of pedv infection	8
and immunofluorescent study on	8
between the nucleocapsid and	8
those induced by ifn	8
and conditions of the	8
of tbk or irf	8
the intestinal lamina propria	8
of porcine deltacoronavirus in	8
to determine the effect	8
virus by duplex rt	8
for min and washed	8
cells were detected in	8
coronavirus related to transmissible	8
spike protein genes reveals	8
vero cells were incubated	8
in the previous study	8
of pedv has been	8
incubated at c for	8
and hpi for qrt	8
virus genome between the	8
but promotes its infectivity	8
epidemic diarrhoea virus infection	8
based on the pedv	8
enterocytes of the small	8
epidemic diarrhea virus transmission	8
encodes four structural proteins	8
author on reasonable request	8
induces antibodies that neutralize	8
genes reveals a polymorphic	8