trigram

This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

trigram	frequency
the presence of	134
as well as	103
for h at	102
at room temperature	100
for min at	84
according to the	75
the use of	74
in this study	74
cd t cells	69
compared to the	63
the cells were	61
in the lungs	60
balb c mice	58
in the presence	58
was added to	57
was used to	57
due to the	52
based on the	52
as shown in	50
in order to	48
acute respiratory syndrome	47
the level of	47
as previously described	47
severe acute respiratory	47
to determine the	46
shown in fig	46
h n influenza	46
influenza virus infection	45
the number of	45
added to the	43
a concentration of	42
the expression of	41
incubated for h	41
influenza a virus	41
the effect of	40
that of the	40
acute lung injury	40
the absence of	39
analysis of the	39
in comparison to	38
respiratory syncytial virus	38
in addition to	38
one of the	37
times with pbs	37
three times with	36
of the pbs	36
respiratory syndrome coronavirus	35
can be used	34
of influenza virus	34
as described previously	33
in the lung	33
the development of	33
as compared to	33
washed three times	33
at a concentration	32
of the virus	32
the plates were	31
east respiratory syndrome	31
the role of	31
h at room	31
in the pbs	31
in the absence	31
middle east respiratory	31
and stored at	30
the pbs group	30
g for min	30
was determined by	30
to the pbs	29
a number of	29
in the case	29
in vitro and	29
and incubated for	29
the case of	29
been shown to	28
in response to	28
data not shown	28
cells in the	28
cells were washed	28
found to be	28
observed in the	28
were infected with	28
changes in the	27
were used to	27
in ml of	27
compared with the	27
those of the	27
there was no	26
on the other	26
thermo fisher scientific	25
of the two	25
vitro and in	25
showed that the	25
the immune response	25
the amount of	25
it has been	25
and in vivo	25
the other hand	25
was observed in	25
cells were incubated	25
to each well	24
than that of	24
were incubated for	24
we found that	24
washed with pbs	24
were treated with	24
expression in the	24
room temperature for	24
was performed using	24
the detection of	23
n influenza virus	23
expression of the	23
and pref alum	23
and incubated at	23
was found to	23
were incubated with	23
of this study	23
the ability of	23
added to each	23
the production of	22
was used as	22
was mixed with	22
in the same	22
approved by the	22
used as a	22
were resuspended in	22
the addition of	22
the lungs of	22
free virus particles	21
the university of	21
were collected from	21
has been shown	21
of the immune	21
high levels of	21
were found to	21
level of igf	21
pi k akt	21
ptm buffer and	21
followed by a	21
as described above	21
with pbs and	21
min at rt	21
diluted in pbs	21
the present study	21
was performed in	21
a variety of	21
caa and ccg	21
the induction of	20
by flow cytometry	20
was carried out	20
for min and	20
the results of	20
the levels of	20
samples were collected	20
cytokines and chemokines	20
mouse hepatitis virus	20
ml of pbs	20
the samples were	20
respiratory distress syndrome	20
the majority of	20
were washed with	20
pei and pei	20
were subjected to	20
used in this	19
associated with the	19
were washed three	19
incubated for min	19
the effects of	19
the mice were	19
acute respiratory distress	19
significantly higher than	19
in a mouse	19
were stained with	19
pr pbs group	19
in accordance with	19
of the lung	19
indicated that the	19
the concentration of	19
entry of iav	19
of the viral	19
cd t cell	19
c mice were	19
the surface of	19
treated pr virus	19
at h post	19
between the two	19
transmissible gastroenteritis virus	19
the importance of	19
mice immunized with	19
and m ko	19
were carried out	18
has been reported	18
of the polymer	18
no significant difference	18
were then washed	18
buffer and lysate	18
the treatment of	18
demonstrated that the	18
role in the	18
cells infected with	18
to confirm the	18
were incubated at	18
sirna nanoparticles were	18
structure of the	18
pref alum immunization	18
the apical surface	18
to evaluate the	18
of the pr	18
of the pm	18
to those of	18
human airway epithelia	18
h at rt	18
when compared to	18
the membrane was	18
linked immunosorbent assay	18
of the target	18
neutralizing antibody titers	18
phosphate buffered saline	18
of mast cells	18
consistent with the	18
the pr pbs	17
shown to be	17
inflammatory lung injury	17
in pbs containing	17
acidic hib crude	17
a range of	17
contribute to the	17
a mouse model	17
lpeg sirna nanoparticles	17
host cell entry	17
were added to	17
was used for	17
the mixture was	17
immunized with pref	17
fetal bovine serum	17
at the same	17
part of the	17
of the mice	17
treated control group	17
was incubated with	17
activity of the	17
were purchased from	17
were analyzed by	17
used for the	17
in pbs for	17
results show that	17
results suggest that	17
results indicate that	16
human mast cells	16
the reaction was	16
and incubated with	16
it was found	16
the supernatant was	16
of the protein	16
honey bee viruses	16
empty expression vector	16
pbs or saline	16
the negative control	16
antisera and f	16
we observed that	16
used in the	16
washed twice with	16
in our study	16
presence of the	16
the efficacy of	16
a total of	16
at xg for	16
of influenza a	16
sa and sa	16
of the anti	16
three independent experiments	16
the generation of	16
mice infected with	16
and mg chinese	16
and stained with	15
as a control	15
the control group	15
c for min	15
was added and	15
in terms of	15
were used for	15
shown in figure	15
were analyzed using	15
for up to	15
to the apical	15
of severe acute	15
in which the	15
with ml of	15
immediately before use	15
the in vitro	15
is required for	15
present in the	15
against h n	15
the pathogenesis of	15
it should be	15
were obtained from	15
the fact that	15
use of a	15
lung injury in	15
the pbs control	15
pr virus was	15
well as the	15
vibrating mesh nebuliser	15
the spike protein	15
mouse model of	15
the end of	15
was found that	15
in the present	15
as described in	15
data indicate that	15
presence of fcs	15
to detect the	15
in inflamed lungs	15
in pbs and	15
analysis of variance	15
world health organization	15
in this work	15
end of the	15
used to detect	15
the combination of	15
cells were then	14
the release of	14
wide range of	14
mice were sacrificed	14
aspiration acute lung	14
the percentage of	14
wako pure chemical	14
xg for minutes	14
the activation of	14
was performed by	14
mice treated with	14
human immunodeficiency virus	14
a mixture of	14
cns resident microglia	14
than those of	14
were collected and	14
western blot analysis	14
are shown in	14
has also been	14
acid aspiration acute	14
to assess the	14
indicating that the	14
the immune system	14
use of the	14
incubated overnight at	14
be used to	14
care and use	14
the prophylactic activity	14
have shown that	14
at and dpi	14
in the cecum	14
like growth factor	14
virus infection in	14
were used as	14
the pei and	14
pure chemical industries	14
a wide range	14
compared with pbs	14
for detection of	14
to the lungs	14
a dose of	14
the h n	14
of middle east	14
in the serum	14
resuspended in pbs	14
was purchased from	14
mg chinese herbs	14
btv ns m	14
prophylactic activity of	14
of the vaccine	14
infection of the	14
in the pr	14
serum samples were	14
been reported to	14
were determined by	13
the context of	13
c for h	13
of the lungs	13
it is possible	13
were detected in	13
the risk of	13
for use in	13
was removed by	13
in honey bees	13
the inhibition of	13
is associated with	13
innate and adaptive	13
indicate that the	13
was measured at	13
was able to	13
was no significant	13
pbs control group	13
the cell surface	13
of the control	13
each sample was	13
no significant differences	13
to obtain a	13
activation of the	13
this study was	13
in contrast to	13
igy and igg	13
mezo et al	13
of the antibody	13
in both the	13
we evaluated the	13
increase in the	13
days after the	13
were performed in	13
found that the	13
with the pbs	13
differences in the	13
levels in the	13
the pi k	13
effect of the	13
the lack of	13
the inflammatory response	13
equine antisera and	13
of ptm buffer	13
solution at a	13
results showed that	13
immune response in	13
in the dark	13
was analyzed by	13
type d betaretroviruses	13
was collected and	13
provided by the	13
development of a	13
suggest that the	13
of the human	13
the synthesis of	13
specific cd t	13
were able to	12
an important role	12
sensitivity and specificity	12
in the context	12
hibiscus tea extract	12
humoral and cellular	12
there were no	12
for the presence	12
infected with btv	12
from patients with	12
and characterization of	12
important role in	12
higher than the	12
of the cells	12
rubisco large subunit	12
the site of	12
was removed and	12
single cell suspensions	12
have been shown	12
of igf mrna	12
with respect to	12
each well and	12
cd and cd	12
most of the	12
for h with	12
mast cells in	12
are known to	12
in vivo imaging	12
plates were incubated	12
each of the	12
be used in	12
a recent study	12
of the experiment	12
of the same	12
were approved by	12
mice in the	12
was detected by	12
in patients with	12
of the most	12
t cells in	12
concentration of the	12
comparison of the	12
such as the	12
the formation of	12
immune responses to	12
blood samples were	12
have been reported	12
and cd t	12
addition to the	12
animal care and	12
play a role	12
as a result	12
tumor necrosis factor	12
immune response to	12
significantly lower than	12
surface of the	12
needs to be	12
a role in	12
be due to	12
cells transfected with	12
were performed using	12
these results suggest	12
of cd t	12
viral load in	12
the rubisco large	12
detection of sars	12
chicken egg yolk	12
in combination with	12
a negative control	12
the first immunization	12
th and th	12
of the antiserum	12
a combination of	12
pr virus activity	12
with regards to	12
the innate immune	12
was resuspended in	12
cell wall proteins	12
the secretion of	11
twice with pbs	11
and the reaction	11
the same time	11
in the stools	11
the administration of	11
influenza virus in	11
com scientificreports www	11
the plasma membrane	11
we did not	11
as a negative	11
gene expression in	11
in the pathogenesis	11
was dissolved in	11
the analysis of	11
on the surface	11
and the cells	11
the activity of	11
in body weight	11
with btv wt	11
determined by the	11
the small intestine	11
recombinant stx b	11
equal volume of	11
with those of	11
were significantly higher	11
pr ppp group	11
genes involved in	11
cells were infected	11
the reaction mixture	11
the type d	11
in a cells	11
thoracic suction window	11
identified in the	11
plates were washed	11
conflict of interest	11
to all wells	11
bovine serum albumin	11
were capable of	11
of neutrophils in	11
and resuspended in	11
by centrifugation at	11
proteins in the	11
mesenteric lymph node	11
which can be	11
was detected in	11
acute inflammatory lung	11
in the bal	11
were stored at	11
mice per group	11
binding to the	11
using the same	11
final concentration of	11
treated with pbs	11
the secondary antibody	11
k akt and	11
the pr ppp	11
the lung tissue	11
specificity of the	11
for the detection	11
virus titers in	11
were centrifuged at	11
the survival rate	11
the course of	11
plates were then	11
the upregulation of	11
genes coding for	11
to obtain the	11
way analysis of	11
because of the	11
and centrifuged at	11
at the end	11
were mixed with	11
a novel coronavirus	11
in infected cells	11
akt and mapk	11
that can be	11
on day post	11
were cultured in	11
administration of hinokitiol	11
is shown in	11
is important to	11
it is important	11
mast cell activation	11
was performed with	11
involved in the	11
with pbs group	11
effect on the	11
analysis was performed	11
detected in the	11
all of the	11
of the pei	11
collected from the	11
and h n	11
compared to pbs	11
response to the	11
the changes in	11
to that of	11
of the cell	10
be used for	10
to reduce the	10
measured at nm	10
uptake in the	10
detection of the	10
of the animals	10
transmissible gastroenteritis coronavirus	10
were washed and	10
experiments were performed	10
was measured by	10
cells were transfected	10
infectious bronchitis virus	10
was evaluated by	10
the protective efficacy	10
the degree of	10
these results show	10
igg and iga	10
the severity of	10
for each sample	10
by western blot	10
there is no	10
sulfadiazine and mg	10
in line with	10
applied to the	10
individual phytoplankton cells	10
of inflammatory cytokines	10
and in vitro	10
was performed as	10
differences between the	10
of a novel	10
cells were cultured	10
daily for days	10
is able to	10
the lactobacillus vaccine	10
by addition of	10
data were analyzed	10
lower than the	10
were transfected with	10
by measuring the	10
the sa mix	10
of the three	10
pr igf group	10
against rabies virus	10
to compare the	10
equipped with a	10
fixed with paraformaldehyde	10
inhibition of igf	10
revealed that the	10
clc main workbench	10
pbs as a	10
responsible for the	10
and purified by	10
were observed in	10
some of the	10
macromolecular prodrugs of	10
it is also	10
was adjusted to	10
to the first	10
data suggest that	10
significant changes in	10
a series of	10
for the development	10
samples were incubated	10
were then incubated	10
pandemic h n	10
then incubated with	10
included in the	10
in each group	10
for quantification of	10
pcr was performed	10
with the same	10
the bsl facility	10
for treatment of	10
and blocked with	10
were coated with	10
significant differences in	10
mapk signaling pathways	10
and expression of	10
than the pbs	10
large subunit of	10
recombinant swinepox virus	10
a final concentration	10
the identification of	10
were inoculated with	10
after the first	10
with a single	10
and mapk signaling	10
were blocked with	10
the large subunit	10
higher than those	10
the pr igf	10
driven host cell	10
at or dpi	10
h n virus	10
similar to those	10
the clc main	10
of the polymers	10
were washed twice	10
cells were fixed	10
in balb c	10
mg ml of	10
it is not	10
for the treatment	10
has not been	10
protein expression in	10
mesenchymal stem cells	10
reaction mixture was	10
the basis of	10
accordance with the	10
the body weight	10
of igf protein	10
the mechanism of	10
added and the	10
virus released in	10
temperature for h	10
for the first	10
for min in	10
of lung injury	10
lymph node cells	10
that mast cells	10
by incubation with	10
to determine if	10
the plate was	10
was associated with	10
their ability to	10
during influenza virus	9
of the infected	9
of fasciola hepatica	9
known to be	9
presence of a	9
found to contain	9
multiplicity of infection	9
with pref advax	9
was set as	9
incubated with the	9
antiviral activity against	9
on the use	9
the pure product	9
similar to the	9
like to thank	9
was consistent with	9
together with the	9
the sections were	9
these results indicate	9
open reading frame	9
ml in pbs	9
the performance of	9
treated with the	9
were monitored for	9
in injured lungs	9
of sirna nanoparticles	9
the concentrations of	9
all animals were	9
is essential for	9
of the bat	9
role of the	9
n influenza infection	9
pfu ml in	9
the wells were	9
in the control	9
pellet was resuspended	9
conjugated goat anti	9
conjugated secondary antibody	9
we showed that	9
the target protein	9
members of the	9
intratracheal administration of	9
to study the	9
used to determine	9
for at least	9
was obtained from	9
was extracted from	9
did not affect	9
groups of mice	9
were grown in	9
the regulation of	9
virus particles are	9
infection in the	9
and incubated in	9
polyacrylamide gel electrophoresis	9
of immune response	9
prepare a solution	9
the fi eld	9
were labeled with	9
and the relative	9
release of the	9
by the animal	9
to remove the	9
of iav entry	9
likely due to	9
with severe acute	9
time quantitative pcr	9
by the addition	9
wells were washed	9
protein in the	9
components of the	9
of acute lung	9
higher levels of	9
in the inflamed	9
with an equal	9
that have been	9
old balb c	9
in pbs buffer	9
min at room	9
egg yolk antibodies	9
of h n	9
the pbs and	9
of neutralizing antibody	9
the apical side	9
mice from the	9
are associated with	9
in the mouse	9
green fluorescent protein	9
response in the	9
in secretory lysosomes	9
overnight at c	9
studies have shown	9
was subjected to	9
comparison to the	9
suggesting that the	9
h before infection	9
the in vivo	9
the pellet was	9
compared to those	9
the choice of	9
the sirna nanoparticles	9
honey bee health	9
were considered significant	9
would like to	9
influenza a viruses	9
a solution at	9
is possible that	9
of the major	9
delivery to the	9
characterization of the	9
the existence of	9
in vivo and	9
vp and vp	9
proliferation in the	9
h n and	9
flow cytometric analysis	9
the binding of	9
h at c	9
the recombinant lactobacillus	9
c bl mice	9
at day postinfection	9
of cytokines and	9
in pbs bsa	9
of the sirna	9
the mucosal immune	9
and cells were	9
the possibility of	9
neutrophils in the	9
growth factor receptor	9
of the tsi	9
of the mouse	9
a standard curve	9
released in secretory	9
ind entry of	9
kindly provided by	9
after h of	9
of the following	9
the culture medium	9
and store them	9
understanding of the	9
mucosal immune response	9
relative to the	9
prior to the	9
protective effect of	9
in the human	9
cell suspensions were	9
the uptake of	9
and use committee	9
the s protein	9
to be the	9
at a dose	9
syncytial virus infection	9
supported by the	9
oral administration of	9
of the peritrophic	9
the extent of	9
delivery of sirna	9
there was a	9
and can be	9
an equal volume	9
the ha head	9
for min to	9
which is the	9
was measured using	9
the results showed	9
images were acquired	9
by confocal microscopy	9
carried out by	9
the utilization of	9
the equine antisera	9
sequence of the	8
min at uc	8
of pas airways	8
and allowed to	8
from each group	8
of honey bee	8
was supported by	8
appears to be	8
been demonstrated to	8
cause of death	8
with regard to	8
human and animal	8
due to their	8
is consistent with	8
avian influenza viruses	8
spike protein of	8
corresponding to the	8
or dpi with	8
two or three	8
performed using the	8
cells in a	8
colony collapse disorder	8
the beginning of	8
then washed three	8
carried out using	8
reported to be	8
authors declare that	8
the growth of	8
mice immunised with	8
results demonstrated that	8
to the wells	8
or empty expression	8
which is a	8
ly g antibody	8
a result of	8
infected with the	8
with an average	8
presence of serum	8
there is a	8
west nile virus	8
ibv spike proteins	8
after the last	8
cov s binding	8
each group were	8
to cells expressing	8
found that there	8
sections were stained	8
carried out in	8
kg body weight	8
were no significant	8
all wells of	8
immunised with sa	8
the observation that	8
lower than that	8
none of the	8
in absence of	8
producing cd t	8
may contribute to	8
of the pulmonary	8
of the genus	8
were fixed with	8
cellular immune responses	8
difference between the	8
large number of	8
the lungs at	8
cells were seeded	8
infection in a	8
of hibiscus tea	8
prodrugs of ribavirin	8
bsa in pbs	8
regards to the	8
nanoparticle tracking analysis	8
and fixed with	8
is that the	8
the application of	8
showed a significant	8
to note that	8
case of the	8
the emergence of	8
derived from the	8
at and days	8
with that of	8
in the intestinal	8
linked to the	8
we analyzed the	8
were collected at	8
and there was	8
photon excitation laser	8
a mtd of	8
pulmonary marginated neutrophils	8
to quantify the	8
related to the	8
an inhibitor of	8
n and h	8
jiang et al	8
the ratio of	8
of equine antisera	8
sheep and cattle	8
of neutralizing antibodies	8
and then washed	8
an increase in	8
was extracted using	8
concentration of mm	8
mice were anesthetized	8
performed as described	8
in the level	8
adaptive immune responses	8
for h and	8
depending on the	8
our data indicate	8
of the drug	8
of the s	8
to trna lys	8
in a humidified	8
dependent on the	8
serum samples from	8
igf protein expression	8
was then removed	8
cell monolayers were	8
is one of	8
female balb c	8
receptor tyrosine kinases	8
the structure of	8
the authors declare	8
was assessed by	8
results of the	8
to investigate the	8
was calculated by	8
and incubated overnight	8
centrifuged at xg	8
a significant reduction	8
human serum albumin	8
were further incubated	8
of incubation at	8
with pbs containing	8
in a refrigerator	8
virus infection and	8
cells were resuspended	8
that they have	8
sars and non	8
survival time of	8
required for the	8
as one of	8
to understand the	8
the type b	8
composition of the	8
to influenza virus	8
well and incubated	8
obtained with the	8
amino acid residues	8
apical surface of	8
the peritrophic matrix	8
to the control	8
were shown to	8
and in the	8
of sulfadiazine and	8
patients with severe	8
of specific antibodies	8
ind iav entry	8
vesicular stomatitis virus	8
hela cells were	8
alignment of the	8
have not been	8
associated with severe	8
fetal calf serum	8
from the e	8
the diversity of	8
or mutant dpp	8
a positive control	8
be noted that	8
of each group	8
in the fi	8
of mouse hepatitis	8
for h to	8
based on a	8
side of the	8
to test for	8
of bovine colostrum	8
induced by the	8
t cells were	8
human monoclonal antibodies	8
can result in	8
transferred to a	8
the inoculum was	8
pbs or il	8
may not be	8
the thoracic suction	8
in all cases	8
murine model of	8
samples were centrifuged	8
to analyze the	8
of infected mice	8
left lungs were	8
decrease in the	8
of the four	8
the ability to	8
at rt with	8
were prepared in	8
wells of the	8
prior to infection	8
the honey bee	8
for hours at	8
blood was collected	8
amino acid sequence	8
we show that	8
this work was	8
of human mast	8
was diluted with	8
a reduction in	8
compared to pref	8
in innate and	8
this study are	8
the cell wall	8
it is likely	8
by using the	8
to enhance the	8
and the plates	8
mhc class i	8
in the study	8
vivo and in	8
supplemental figure a	8
of the different	8
on the basis	8
the potential of	8
load in the	8
immune response against	8
a virus infection	8
has been demonstrated	8
were monitored daily	8
following pr infection	8
could not be	8
to mg ml	8
of rabies virus	8
the timer for	8
sera from the	8
removed and the	8
well as in	8
should be noted	8
were anesthetized with	8
of the recombinant	8
in our studies	7
protein assay kit	7
group was significantly	7
we demonstrated that	7
for in vivo	7
rates of photosynthesis	7
pref alone and	7
wt or mutant	7
and cellular immune	7
the delivery of	7
suggested that the	7
the host cell	7
its ability to	7
a control group	7
the elisa plate	7
hemolytic uremic syndrome	7
between unbound and	7
using the following	7
is known to	7
the respiratory tract	7
u ml penicillin	7
suggests that the	7
mammary tumor virus	7
the lungs were	7
of facs buffer	7
supernatants were harvested	7
serial dilutions of	7
and adaptive immune	7
we describe the	7
the study of	7
after tgev infection	7
of the h	7
total number of	7
of the individual	7
described in the	7
to this end	7
antibodies were detected	7
effects of the	7
was significantly lower	7
in h n	7
is likely that	7
it is a	7
were dissolved in	7
hibiscus sabdariffa l	7
milk in pbs	7
of blocking agents	7
was removed from	7
obtained from the	7
we used a	7
effect on t	7
lung tissue was	7
it does not	7
airway epithelial cells	7
respiratory tract infections	7
considered to be	7
production in the	7
the inflamed lungs	7
there are no	7
we investigated the	7
turn on the	7
ph of the	7
immunodeficiency virus type	7
determined to be	7
in mice immunized	7
e and e	7
immune recognition of	7
by at least	7
and the corresponding	7
function of the	7
efficacy of the	7
spectrum antiviral activity	7
for minutes at	7
minutes at room	7
a moi of	7
dep and dyna	7
have been described	7
them in a	7
recognition of the	7
of mg kg	7
spectrum antiviral agents	7
institutional animal care	7
of the antigen	7
were prepared by	7
was also observed	7
been linked to	7
of caa and	7
of target cells	7
of the gene	7
the difference in	7
with respiratory syncytial	7
the titer of	7
weight loss of	7
examination of the	7
and approved by	7
to interact with	7
is responsible for	7
were randomly divided	7
of pbs as	7
in the infected	7
the nebulisation of	7
our understanding of	7
evaluation of the	7
the mesenteric lymph	7
to a final	7
mg ml in	7
of the plate	7
the diagnosis of	7
total rna was	7
the world health	7
using graphpad prism	7
the viral load	7
disease control and	7
plates in a	7
expression vectors for	7
the sirna nanoparticle	7
of the negative	7
randomly divided into	7
cells were grown	7
represented as mean	7
the ph of	7
resulted in a	7
involved in innate	7
pbs for h	7
treatment was given	7
of individual phytoplankton	7
antibody in the	7
coding for the	7
to investigate whether	7
shaking at rpm	7
followed by incubation	7
followed by cycles	7
the amino acid	7
in indirect elisa	7
are represented as	7
in the supernatant	7
supplementary figure a	7
of a recombinant	7
schade et al	7
of pref alum	7
been reported that	7
was quantified using	7
comparison to pbs	7
that in the	7
the blank control	7
add l of	7
the primary immunization	7
and the supernatant	7
absorbance was measured	7
supplementary materials figure	7
result in a	7
the assembly of	7
washed five times	7
contributed to the	7
the protein expression	7
pbs group was	7
dose of mg	7
that there were	7
were labelled with	7
was observed when	7
the env gene	7
bound m ko	7
mice were treated	7
of mice was	7
the cells are	7
culture supernatants were	7
mast cells are	7
the pbs controls	7
show that the	7
compared with those	7
factor receptor tyrosine	7
balf and serum	7
they have no	7
appear to be	7
of the corresponding	7
samples were then	7
course of the	7
inactivated pr virus	7
mg chinese herb	7
overall clinical scores	7
binding of the	7
were prepared for	7
was subtracted from	7
control and prevention	7
is an important	7
in our laboratory	7
mg ml and	7
from mice immunised	7
were harvested and	7
the specificity of	7
of specific igy	7
in case of	7
declare that they	7
significant reduction of	7
unbound and bound	7
the sequences of	7
these data suggest	7
was performed to	7
food and water	7
inhibitory effect on	7
to be a	7
the membrane is	7
been added to	7
were expressed as	7
medium was replaced	7
signs of disease	7
was used in	7
group were significantly	7
expression level of	7
the genus betaretrovirus	7
materials figure s	7
the interaction of	7
against the target	7
at hours after	7
reduction in the	7
igf mrna expression	7
purchased from the	7
content of the	7
from the infected	7
per group were	7
the total number	7
splenocytes from mice	7
along with the	7
control group was	7
the following parameters	7
in naive mice	7
current protocols in	7
dna vaccine constructs	7
the most important	7
values of the	7
immune responses in	7
in agreement with	7
for disease control	7
infected with pr	7
the structure and	7
to examine the	7
when compared with	7
the contribution of	7
regions of the	7
genbank accession number	7
the influenza virus	7
lead to the	7
the form of	7
mast cells may	7
all mice were	7
were normalized to	7
upper respiratory tract	7
due to its	7
and igg antibodies	7
th cd t	7
difference in the	7
alone and pref	7
a solution of	7
over the course	7
infiltration in the	7
mice were injected	7
in splenocytes from	7
interactions between proteins	7
a and b	7
the target antigen	7
and it is	7
cytokines in the	7
work was supported	7
protein corona formation	7
of egg yolk	7
at early stages	7
reported that the	7
of the innate	7
considered statistically significant	7
resuspended in ml	7
protective efficacy of	7
acute paralysis virus	7
timer for hr	7
gondii rh strain	7
survival rate of	7
which does not	7
the pbs or	7
at a moi	7
store them in	7
downstream of the	7
of mm in	7
of ebola virus	7
samples from the	7
levels of the	7
virus activity of	7
mucosal immune responses	7
of hibiscus sabdariffa	7
rna was extracted	7
was centrifuged at	7
was performed at	7
of the inflammatory	7
protocols in microbiology	7
in weekly intervals	7
the protective effect	7
in developing countries	7
may be more	7
ha titer of	7
kappa light chain	7
volume of pbs	7
in this regard	7
is the main	7
room temperature in	7
with a pbs	7
type b and	7
synthesis of the	7
dilution of the	7
of injection site	7
in the development	7
and persistence of	7
of the gag	7
following rsv challenge	7
induction of macropinocytosis	7
combination of ptm	7
with fetal bovine	7
coli lysate and	7
significant increase in	7
in the spleen	7
clinical symptoms of	7
a few seconds	7
in pulmonary marginated	7
the protein concentration	7
can be easily	7
expression levels in	7
inhibitory effect of	7
in a murine	7
with l of	7
with the exception	7
of the mabs	7
of shiga toxin	6
of ptm and	6
a model of	6
membrane was incubated	6
neutrophils in inflamed	6
times that of	6
were seeded in	6
in the injured	6
from the ha	6
the most common	6
pbs for min	6
antiviral activity of	6
were detected using	6
with the following	6
mice were immunized	6
cardiogenic pulmonary edema	6
be statistically significant	6
innate immune recognition	6
analyses were performed	6
from the apical	6
were incubated in	6
plates were coated	6
or in the	6
stx b subunit	6
water ad libitum	6
the method of	6
influenza viruses in	6
were scored as	6
an in vivo	6
of the host	6
been associated with	6
central nervous system	6
times with pbst	6
was conducted using	6
elisa for the	6
purified rubisco from	6
with a ph	6
to be statistically	6
were designed to	6
the transcription of	6
were applied to	6
immunized mice and	6
and spinal cord	6
transfected with expression	6
cell line a	6
in influenza virus	6
gene expression patterns	6
dialyzed against pbs	6
flow cytometry and	6
half of the	6
acute sars and	6
that there was	6
the characteristics of	6
expression of a	6
all other groups	6
response of the	6
role of il	6
the viral titer	6
were compared to	6
mice treated by	6
the protein level	6
determined by elisa	6
of the genome	6
of inflammatory cells	6
more than one	6
nanoparticles based on	6
harvested by centrifugation	6
swinepox virus expressing	6
of microglia in	6
of the membrane	6
pei sirna nanoparticle	6
pmol photons m	6
at early time	6
higher than that	6
specific igg a	6
using a multichannel	6
well and the	6
no competing interests	6
gene transfer to	6
collected by centrifugation	6
the phage titres	6
levels of ifn	6
after initial immunisation	6
results indicated that	6
shown are the	6
paraformaldehyde in pbs	6
nps based on	6
the frequency of	6
referred to as	6
three times for	6
and the protein	6
after which the	6
the selection of	6
whole inactivated vaccine	6
washed times with	6
injury in the	6
h after infection	6
to infection with	6
our previous study	6
resulting in a	6
used as the	6
validation of the	6
by the manufacturer	6
place plates in	6
surface expression of	6
first line of	6
accounted for by	6
full speed in	6
in the murine	6
do not exceed	6
of the primary	6
in the elisa	6
previous studies have	6
responses in the	6
the connecting tube	6
anesthetized with ketamine	6
be considered as	6
was applied to	6
not appear to	6
as measured by	6
hematoxylin and eosin	6
at the protein	6
innate immune response	6
wang et al	6
few seconds in	6
were placed in	6
of mg ml	6
at least h	6
supplementary figure c	6
comparison with the	6
virus was mixed	6
human endogenous retrovirus	6
in vitro in	6
iga in the	6
was expressed as	6
in escherichia coli	6
and mg l	6
simultaneous detection of	6
mrna and protein	6
to pbs controls	6
was determined as	6
were examined by	6
of infectious evs	6
was conducted in	6
role of mast	6
ml of the	6
avian infectious bronchitis	6
of proinflammatory cytokines	6
recognition of eap	6
the spinal cord	6
the day of	6
is known about	6
was shown to	6
subjected to sds	6
the treatment was	6
in the training	6
and dialyzed against	6
was tested by	6
infected mice were	6
the first line	6
statistical analyses were	6
responses induced by	6
major histocompatibility complex	6
significant difference between	6
were taken at	6
the stools of	6
a final volume	6
of the e	6
a score of	6
were measured by	6
igf mrna and	6
in our experience	6
the polyclonal antiserum	6
the integrity of	6
significant difference was	6
low ph environment	6
were taken from	6
studies showed that	6
were maintained in	6
the expression level	6
washing with pbs	6
serial dilution of	6
observed between the	6
chinese herb groups	6
was higher than	6
bronchial epithelial cells	6
using flow cytometry	6
protein of interest	6
the mice in	6
and the use	6
brain and spinal	6
at full speed	6
the sample was	6
to identify the	6
to determine whether	6
of variance with	6
the control groups	6
virus replication and	6
capping protein flid	6
conformation of the	6
total volume of	6
and kept at	6
an analysis of	6
is a major	6
and water ad	6
recombinant lactobacillus vaccine	6
the antibodies were	6
and the other	6
animal ethics committee	6
blank control group	6
hepatitis c virus	6
of inflammation in	6
used instead of	6
h of incubation	6
mhc class ii	6
and lung tissues	6
id g in	6
pro vibrating mesh	6
positive and negative	6
study showed that	6
animal experiments were	6
the vibrating mesh	6
the acidic ph	6
with empty expression	6
performed as previously	6
control mice were	6
was collected by	6
human mesenchymal stem	6
airway epithelia were	6
according to a	6
had no effect	6
the serum and	6
confocal laser scanning	6
and sa peptides	6
elisas based on	6
born from immunised	6
any of the	6
with an anti	6
the gene expression	6
for a few	6
pathogenic h n	6
of the serum	6
type and th	6
to the induction	6
speed in a	6
final volume of	6
we conclude that	6
antibody phage display	6
sa or sa	6
in the mesenteric	6
a a a	6
as a vaccine	6
centrifuged for min	6
can be stored	6
bind to the	6
of the capsids	6
cryptosporidium parvum infection	6
of igy against	6
the first plate	6
provide protection against	6
vector dna was	6
of mast cell	6
of inflammation and	6
is the first	6
viral transport media	6
divided into four	6
changes in body	6
the difference between	6
the detection limit	6
of dna vaccine	6
identification of the	6
as a potential	6
to account for	6
five times with	6
on iav entry	6
microglia in the	6
genes that were	6
upstream of the	6
lung tissue of	6
and release of	6
the control cells	6
to overcome the	6
may provide a	6
that it is	6
as and the	6
lungs were harvested	6
samples were taken	6
with a recombinant	6
nanoparticle uptake in	6
an arrest of	6
by phage display	6
increasing concentrations of	6
in adult mice	6
chinese herbs could	6
the two colonies	6
in a microcentrifuge	6
is based on	6
and a major	6
that they are	6
the nature of	6
incubated at room	6
to host cells	6
below the detection	6
the potential to	6
of monoclonal antibody	6
to estimate the	6
lung injury and	6
diverse range of	6
was based on	6
in the future	6
high titers of	6
infection in balb	6
as soon as	6
infected cells were	6
the exception of	6
skin of injection	6
dependent cellular cytotoxicity	6
via flow cytometry	6
inactivating agent for	6
a and a	6
in the gut	6
by the presence	6
uptake of fdx	6
of the other	6
iav entry pathways	6
to develop a	6
higher level of	6
the vaccine group	6
and specificity of	6
mm edta in	6
pathogenic avian influenza	6
body weight of	6
are available from	6
does not affect	6
the size of	6
and used for	6
then washed with	6
graphpad prism software	6
was observed by	6
a multiplicity of	6
are based on	6
as in the	6
an average weight	6
automated plate washer	6
stx b igy	6
immune response elicited	6
immunity of pref	6
antibody titers in	6
the impact of	6
of igf in	6
ebola virus infection	6
a critical role	6
an automated plate	6
supernatant was collected	6
culture medium was	6
in blood and	6
model of acute	6
the absorbance was	6
model of campylobacter	6
sin nombre virus	6
in mice and	6
of dna vaccines	6
of the full	6
t cells and	6
the pm is	6
the involvement of	6
the replication of	6
mouse mammary tumor	6
with shaking at	6
was set to	6
concentration in the	6
the binding affinity	6
found to have	6
highly pathogenic avian	6
performed using a	6
previously described methods	6
well as a	6
and then the	6
days prior to	6
of the tgev	6
we tested the	6
of airway epithelia	6
majority of the	6
pbs was added	6
it can be	6
were similar to	6
washes with pbs	6
as opposed to	6
supplementary figure b	6
a significant increase	6
was performed on	6
intraperitoneally injected with	6
as part of	6
of toxoplasma gondii	6
at dpi and	6
in gene expression	6
the evolution of	6
influenza virus hemagglutinin	6
in young children	6
in comparison with	6
well of the	6
of infected cells	6
fold serial dilutions	6
has been previously	6
for inflamed lungs	6
antibody titers were	6
we sought to	6
this suggests that	6
on day after	6
an immune response	6
mixture was diluted	6
passive transfer of	6
the sensitivity of	6
as the mean	6
bca protein assay	6
has been suggested	6
ml in ml	6
the supernatants were	6
the antiviral activity	6
results in a	6
rat model of	6
of the mixture	6
of the first	6
identification of a	6
igg a igg	6
the binding capacity	6
pbs containing fcs	6
from rsv infection	6
in pbs controls	6
were used in	6
out of the	6
days after infection	6
of this article	6
of genes involved	6
results of this	6
the clinical symptoms	6
of initial dose	6
be used as	6
and hibiscus acid	6
was significantly decreased	6
in the early	6
of pref advax	6
was confirmed by	6
followed by three	6
to remove any	6
using an automated	6
hib crude was	6
of the sample	6
the other groups	6
mouse and human	6
of lung tissue	6
at days post	6
infection in mice	6
of immune responses	6
at c with	6
of viral proteins	6
the cause of	6
to the antigen	6
incubated with a	6
concentration was determined	6
that the majority	6
significant increases in	6
and a p	6
a plate reader	6
the interaction between	6
were analysed with	6
the onset of	6
percentage of pas	6
photon excitation microscopy	6
immunized mice were	6
attached to the	6
type immune responses	6
an inflammatory response	6
or in pbs	6
blocked with bsa	6
siga and siga	6
centers for disease	6
led to a	6
set as and	6
x g for	6
infection with btv	6
type of immune	6
immunized mice had	6
at a multiplicity	6
and mg ml	6
the possibility that	6
the kinetics of	6
were measured in	6
are the most	6
ige sensitization alone	6
with expression vectors	6
it may be	6
concentrated in the	6
amino acid sequences	6
all the samples	6
rabies dna vaccine	6
in a single	6
plays an important	6
mdp and tuftsin	6
a multichannel pipettor	6
were prepared using	6
c and g	6
be required to	6
c and il	6
the training set	6
of transmissible gastroenteritis	6
amino acid substitution	6
was collected from	6
of the env	6
triggered by fc	6
in the in	6
k akt signaling	6
was isolated from	6
dpp surface expression	6
by cycles of	6
development of novel	6
study are available	6
lung injury was	6
and immune sera	6
are representative of	6
in each well	6
m ko phage	6
expression of genes	6
the corresponding author	6
of btv unag	6
during acute inflammation	6
from immunised mothers	6
linear discriminant analysis	6
in pbs to	6
significant decrease in	6
memory b cells	6
a change in	6
responses to iapv	6
integrity of the	6
are presented as	6
in the lower	6
group at dpi	6
high concentration of	6
the strength of	6
the need for	6
mg kg body	6
further incubated for	6
to the lung	6
binding to dpp	6
the protein of	6
effect of eap	6
we assessed the	6
was significantly higher	6
value of the	6
significant difference in	6
by in vitro	6
basis of the	6
average weight loss	6
with pref alum	6
were detected by	6
beginning of the	6
was detected using	6
can be found	6
lpeg sirna nanoparticle	6
and western blot	6
or saline and	6
the same volume	6
study was to	6
were examined for	6
immune responses and	6
can directly infect	6
in the first	6
study of the	6
h and h	6
up to weeks	6
and pref advax	6
was performed for	6
of mice were	6
sensitivity of the	6
in our previous	6
of gene expression	6
was observed with	6
not accumulate in	6
were prepared from	6
intranasal administration of	6
a large number	6
a density of	6
highly pathogenic h	6
materials and methods	6
in all the	6
purchased from sigma	6
low levels of	6
were harvested from	6
with pref alone	6
for the elisa	6
the conformation of	6
for patients with	6
edta in pbs	6
both the pbs	6
facs diva software	6
unbound m ko	6
yielding the pure	6
may also be	6
in the induction	6
virus in the	6
in dh o	5
little is known	5
was found in	5
polyphosphates and polyphosphonates	5
interaction with the	5
ability of the	5
the most abundant	5
confirmed that the	5
the results were	5
serve as a	5
food and drug	5
g ml of	5
from all samples	5
at least ml	5
of the spike	5
the peritrophic membrane	5
of monoclonal antibodies	5
infected with oocysts	5
production of il	5
the cover glass	5
infected with f	5
were tested in	5
transferred onto a	5
in liquid nitrogen	5
were identified by	5
total cell lysate	5
did not accumulate	5
mice in each	5
to a lesser	5
we examined the	5
treated group showed	5
virus in a	5
did not result	5
for each of	5
transfected with empty	5
were observed for	5
various concentrations of	5
affinity for the	5
the classification algorithm	5
as shown by	5
mucosal delivery of	5
of a specific	5
eluted m ko	5
to all other	5
screening of the	5
mice were euthanized	5
the findings of	5
all the constructs	5
different cell types	5
the utility of	5
saturated rates of	5
a dilution of	5
the ribosomal biogenesis	5
to inhibit the	5
and class ii	5
laser scanning microscopy	5
into groups of	5
for days prior	5
used to develop	5
as previously reported	5
during mast cell	5
analysis was carried	5
interaction of the	5
and protein expression	5
in pbs or	5
the livers of	5
patients with acute	5
injected with pbs	5
costs of existence	5
with pbs to	5
at a final	5
after incubation with	5
were kept in	5
group did not	5
are listed in	5
number of genes	5
that is the	5
extent to which	5
response elicited by	5
the integrated density	5
protective immunity against	5
the siga format	5
and the mixture	5
min at full	5
lsr ii flow	5
evaluation of a	5
on the day	5
was blocked with	5
analyzed by flow	5
ppp group was	5
with the sa	5
were plated in	5
h after the	5
mutant dpp was	5
vivo imaging of	5
of the igy	5
nanoparticle or protein	5
concentrations of ethanol	5
coronavirus associated with	5
be associated with	5
were incubated overnight	5
and washed with	5
pellets were resuspended	5
the survival time	5
were fed with	5
and subjected to	5
the composition of	5
in the mm	5
the viral rna	5
versions of the	5
at day p	5
were stimulated with	5
after centrifugation at	5
of the solution	5
gene expression profiles	5
dietary intake of	5
was determined using	5
the first time	5
by the bradford	5
virus particles in	5
and h after	5
and type d	5
ii flow cytometer	5
error bars indicate	5
nahco na co	5
universal influenza vaccine	5
at to dpi	5
all animal experiments	5
g in the	5
biodistribution and persistence	5
play an important	5
receptor for the	5
major cause of	5
purified using the	5
and control groups	5
ml of purified	5
was observed that	5
region of the	5
and the total	5
adoptive transfer of	5
page and western	5
in this context	5
innate immune system	5
coupled to a	5
in vivo lung	5
were acquired with	5
no change in	5
the x strain	5
the sequence gb	5
in the immune	5
dbco per igg	5
consistent with this	5
for min with	5
rfhpcl and rfhpcl	5
the wells and	5
ultra centrifugal filter	5
for pulmonary delivery	5
sealed and the	5
than the unmodified	5
the membranes were	5
of dpp surface	5
and gene expression	5
are expressed as	5
dry lung weight	5
a key role	5
the lung is	5
lower respiratory tract	5
in the form	5
preliminary experiments s	5
diluted with a	5
of pneumococcal pneumonia	5
time of mice	5
min and the	5
at different times	5
the injured lungs	5
was tested in	5
the lungs and	5
response induced by	5
is expected to	5
at least two	5
h n avian	5
activity of orally	5
growth medium containing	5
level of the	5
the viral proliferation	5
universal influenza vaccines	5
mice were monitored	5
be explained by	5
cytotoxic t lymphocytes	5
that lead to	5
was determined from	5
expression of il	5
of chicken egg	5
a vibrating mesh	5
influenza virus and	5
the range of	5
as a positive	5
at least in	5
room temperature with	5
as determined by	5
cloned into the	5
infect airway epithelia	5
to the non	5
the aim of	5
scores were averaged	5
cells were lysed	5
with btv ns	5
in the binding	5
of the cross	5
at the university	5
mice of the	5
were intraperitoneally injected	5
the splenic lymphocytes	5
blood and tissues	5
traditional chinese medicine	5
cells expressing the	5
the ampule was	5
were evaluated by	5
and a mtd	5
result not shown	5
b and type	5
not statistically significant	5
pbs complementary to	5
to our knowledge	5
from each mouse	5
size exclusion chromatography	5
with the pei	5
size of the	5
gene expression data	5
epitope of the	5
was scored as	5
mice receiving pbs	5
the first step	5
same volume of	5
in this case	5
protection against h	5
a gammaretroviral env	5
shiga toxin b	5
immune cells in	5
transmission electron microscope	5
polymorphisms in dpp	5
fold dilutions of	5
was calculated as	5
supernatants were collected	5
ii and pbs	5
inhibition of the	5
t containing milk	5
antibacterial activity of	5
use of trna	5
presence or absence	5
method for the	5
for serodiagnosis of	5
that does not	5
the ptm buffer	5
is imperative to	5
was a significant	5
the incidence of	5
alveolar epithelial cells	5
were analyzed in	5
and approved the	5
of the genes	5
of human and	5
similar levels of	5
by fluorescence microscopy	5
coma et al	5
four hours after	5
were washed in	5
to allow for	5
plates at a	5
whereas the inhibition	5
protein of sars	5
days post infection	5
when formulated with	5
the mixture of	5
the product was	5
mediated acute inflammatory	5
is independent of	5
neutralizing antibody responses	5
we demonstrate that	5
strain of mhv	5
the last immunization	5
was determined to	5
fold increase in	5
infected cells and	5
induced lung injury	5
binding capacity of	5
the length of	5
in the hib	5
memory b cell	5
the discovery of	5
in balf and	5
mice were administered	5
the geometric mean	5
the control of	5
temperature for minutes	5
in ml for	5
enhanced green fluorescent	5
statistical significance of	5
was flame sealed	5
and cellular immunity	5
as a consequence	5
are expected to	5
it was observed	5
investigated in a	5
and analysis of	5
could be a	5
neutralizing antibody production	5
investigated the potential	5
were collected for	5
the n p	5
possible that the	5
by norbury et	5
in pbs was	5
has to be	5
free protein formulation	5
c for s	5
significant differences between	5
the genome of	5
inhibition of dyna	5
were predicted using	5
line of defense	5
and adaptive immunity	5
s binding to	5
cellular immune response	5
cov s to	5
mm in sterile	5
rpm for min	5
assembly of the	5
this can be	5
have been widely	5
of the fluorescence	5
not significantly different	5
nls and nes	5
did not significantly	5
were transferred to	5
was treated with	5
were obtained after	5
at h after	5
three washes with	5
in vivo behavior	5
for hour at	5
solution to all	5
evs or free	5
immune response was	5
ptm and e	5
l of the	5
we explored the	5
time to death	5
the antibody fragments	5
mg ml was	5
of antibodies against	5
on influenza virus	5
the highest dilution	5
addition of the	5
allantoic fluid was	5
used for immunization	5
the difference was	5
to the other	5
up to months	5
a single oral	5
containing virus particles	5
humoral and cell	5
of the elisa	5
show that jcdv	5
of influenza infection	5
in triplicate and	5
information on the	5
bacteria in the	5
of the assay	5
d in ml	5
m urea buffer	5
infection in vivo	5
early stages of	5
the lysosomal markers	5
pbs with glycerol	5
fixed and stained	5
igf group was	5
sensitization and fc	5
to low ph	5
at least three	5
by ige sensitization	5
and pbs with	5
the proteins in	5
performed before the	5
the tissue culture	5
and treatment of	5
m ko were	5
the major histocompatibility	5
were selected for	5
of the mock	5
a comparison of	5
no significant changes	5
cell proliferation and	5
digital gene expression	5
pbs and fixed	5
a epitope of	5
as inhibitors of	5
isolated from the	5
expressed as the	5
to the different	5
in figure b	5
prior to use	5
the management of	5
and phage preincubation	5
in a dose	5
enterohemorrhagic escherichia coli	5
a function of	5
analyzed using the	5
increased in the	5
data are represented	5
in the range	5
m ko was	5
in this paper	5
that of pbs	5
we could not	5
the polymer and	5
were intratracheally infected	5
a murine model	5
the viral envelope	5
in the virus	5
sequences of the	5
after removal of	5
a significant dose	5
used in combination	5
antibody response was	5
whether or not	5
the animals were	5
the virus was	5
as a model	5
cells were further	5
at the plasma	5