This is a table of type trigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.
trigram | frequency |
---|---|
mediated isothermal amplification | 750 |
the detection of | 234 |
rapid detection of | 207 |
for the detection | 187 |
for detection of | 149 |
reverse transcription loop | 140 |
in this study | 133 |
of the rt | 132 |
for rapid detection | 119 |
the use of | 111 |
polymerase chain reaction | 109 |
as well as | 100 |
nucleic acid amplification | 96 |
the sensitivity of | 93 |
severe acute respiratory | 91 |
acute respiratory syndrome | 89 |
a license to | 88 |
to display the | 88 |
display the preprint | 88 |
who has granted | 88 |
license to display | 88 |
has granted medrxiv | 88 |
granted medrxiv a | 88 |
medrxiv a license | 88 |
the preprint in | 88 |
the copyright holder | 86 |
the lamp reaction | 85 |
holder for this | 85 |
copyright holder for | 85 |
the presence of | 85 |
isothermal amplification assay | 83 |
of the lamp | 83 |
detection of sars | 82 |
according to the | 82 |
isothermal amplification method | 82 |
the lamp assay | 82 |
the author funder | 78 |
is the author | 78 |
lamp assay was | 77 |
this version posted | 77 |
isothermal amplification for | 75 |
which was not | 75 |
f and b | 75 |
respiratory syndrome coronavirus | 75 |
based on the | 72 |
sensitivity of the | 70 |
sensitivity and specificity | 70 |
detection limit of | 69 |
amplification of dna | 66 |
by peer review | 65 |
was not certified | 65 |
certified by peer | 65 |
not certified by | 65 |
in order to | 64 |
the specificity of | 63 |
isothermal amplification of | 60 |
sybr green i | 60 |
lamp assay for | 59 |
can be used | 58 |
c for min | 58 |
as shown in | 57 |
loopmediated isothermal amplification | 55 |
the development of | 55 |
detection of the | 55 |
and evaluation of | 55 |
assay for the | 55 |
development of a | 55 |
specificity of the | 55 |
notomi et al | 54 |
ibv and ndv | 53 |
loop mediated isothermal | 53 |
due to the | 53 |
agarose gel electrophoresis | 52 |
the results of | 52 |
time reverse transcription | 51 |
preprint in perpetuity | 50 |
rapid diagnosis of | 50 |
development and evaluation | 50 |
amplification assay for | 49 |
the detection limit | 48 |
the n gene | 48 |
visual detection of | 48 |
the lamp method | 48 |
evaluation of a | 47 |
for this preprint | 47 |
used for the | 47 |
the need for | 46 |
the diagnosis of | 45 |
a total of | 45 |
found to be | 45 |
amplification method for | 45 |
under isothermal conditions | 44 |
to determine the | 44 |
limit of detection | 44 |
was found to | 43 |
of the target | 42 |
a set of | 42 |
a reverse transcription | 42 |
by reverse transcription | 42 |
the number of | 41 |
was carried out | 41 |
method for rapid | 41 |
fip and bip | 40 |
new england biolabs | 40 |
of novel coronavirus | 40 |
primers were designed | 40 |
one of the | 40 |
this study was | 39 |
preprint this version | 39 |
this preprint this | 39 |
of autophagy in | 39 |
was used as | 39 |
of a novel | 39 |
this this version | 38 |
for this this | 38 |
rapid detection and | 38 |
the reaction mixture | 37 |
of severe acute | 37 |
specificity and sensitivity | 37 |
been shown to | 37 |
were used to | 37 |
bst dna polymerase | 37 |
that the rt | 36 |
in the reaction | 36 |
nucleic acid sequence | 36 |
of the reaction | 36 |
used in this | 36 |
rapid and sensitive | 36 |
nucleic acid testing | 36 |
lamp assay and | 36 |
the performance of | 35 |
assay for detection | 35 |
that of the | 35 |
positive and negative | 35 |
was added to | 35 |
for the diagnosis | 34 |
have been developed | 34 |
for the rapid | 34 |
shown in table | 34 |
added to the | 34 |
used in the | 34 |
detection of mycobacterium | 33 |
of reverse transcription | 33 |
detection of human | 33 |
amplification for rapid | 33 |
parida et al | 33 |
lamp assay is | 33 |
in the presence | 32 |
shown in fig | 32 |
of mycobacterium tuberculosis | 32 |
japanese encephalitis virus | 31 |
of nucleic acid | 31 |
was performed using | 31 |
has been shown | 31 |
serial dilutions of | 31 |
a and b | 31 |
of nucleic acids | 31 |
the present study | 31 |
the reaction was | 31 |
used to detect | 31 |
for diagnosis of | 30 |
the target dna | 30 |
west nile virus | 30 |
of japanese encephalitis | 30 |
in the field | 30 |
assay for rapid | 30 |
isothermal amplification reaction | 30 |
used as a | 30 |
all rights reserved | 30 |
method for the | 29 |
more sensitive than | 29 |
reuse allowed without | 29 |
for min and | 29 |
no reuse allowed | 29 |
and specificity of | 29 |
allowed without permission | 29 |
be used for | 29 |
was used to | 29 |
were collected from | 29 |
the naked eye | 28 |
the limit of | 28 |
is made available | 28 |
can be detected | 28 |
to the reaction | 28 |
rna was extracted | 28 |
detection and differentiation | 28 |
international license it | 28 |
be used to | 28 |
in the lamp | 28 |
for the rt | 28 |
available under a | 28 |
lamp and rt | 28 |
has been used | 28 |
recombinase polymerase amplification | 28 |
license it is | 28 |
it is made | 28 |
magnetic bead rna | 28 |
was extracted from | 28 |
made available under | 28 |
and differentiation of | 28 |
because of the | 27 |
version posted november | 27 |
a rapid and | 27 |
limit of the | 27 |
sensitive detection of | 27 |
role of autophagy | 27 |
zhang et al | 27 |
preprint the copyright | 27 |
detection of viral | 27 |
of infectious diseases | 26 |
isothermal nucleic acid | 26 |
than that of | 26 |
to evaluate the | 26 |
a detection limit | 26 |
at room temperature | 26 |
results of the | 26 |
influenza a viruses | 26 |
nucleic acid detection | 26 |
at c for | 26 |
of influenza a | 26 |
hepatitis c virus | 26 |
feline infectious peritonitis | 26 |
in the rt | 26 |
was used for | 26 |
the rapid detection | 26 |
of clinical samples | 26 |
the spread of | 26 |
detection of novel | 26 |
compared to the | 26 |
j o u | 25 |
o o f | 25 |
in addition to | 25 |
of the virus | 25 |
lamp reaction was | 25 |
of the assay | 25 |
p r o | 25 |
shown in figure | 25 |
p r e | 25 |
using loop primers | 25 |
lamp products were | 25 |
n a l | 25 |
r o o | 25 |
u r n | 25 |
o u r | 25 |
and can be | 25 |
to detect the | 25 |
of dengue virus | 25 |
the target sequence | 25 |
r n a | 25 |
a l p | 24 |
for min at | 24 |
l p r | 24 |
a novel loop | 24 |
showed that the | 24 |
it has been | 24 |
mori et al | 24 |
high sensitivity and | 24 |
has also been | 24 |
in clinical samples | 24 |
can be performed | 24 |
performance of the | 24 |
direct detection of | 24 |
for rapid diagnosis | 24 |
isothermal amplification using | 24 |
east respiratory syndrome | 23 |
accelerated reaction by | 23 |
to that of | 23 |
samples with a | 23 |
the formation of | 23 |
a water bath | 23 |
a constant temperature | 23 |
isothermal amplifi cation | 23 |
can also be | 23 |
middle east respiratory | 23 |
were used as | 23 |
time detection of | 23 |
detection of severe | 23 |
porcine circovirus type | 23 |
of zika virus | 23 |
was performed in | 23 |
the e gene | 23 |
is based on | 23 |
a number of | 23 |
in a single | 23 |
were obtained from | 23 |
we developed a | 23 |
of the samples | 23 |
samples were collected | 22 |
from patients with | 22 |
of the primers | 22 |
carried out in | 22 |
a variety of | 22 |
and quantification of | 22 |
bead rna extraction | 22 |
performed in a | 22 |
does not require | 22 |
and rapid detection | 22 |
to develop a | 22 |
for nucleic acid | 22 |
a simple and | 22 |
the role of | 22 |
amplification and detection | 22 |
herpes simplex virus | 22 |
rapid and real | 22 |
mediated isothermal amplifi | 22 |
for min in | 22 |
simple and rapid | 22 |
that can be | 21 |
version posted july | 21 |
eiken chemical co | 21 |
end of the | 21 |
detection of porcine | 21 |
clinical diagnosis of | 21 |
armored rna particles | 21 |
approved by the | 21 |
amplification using loop | 21 |
pdm h n | 21 |
and sensitive detection | 21 |
copies of the | 21 |
lamp and pcr | 21 |
lamp has been | 21 |
by using the | 21 |
has been reported | 21 |
the optimal reaction | 21 |
were carried out | 21 |
data not shown | 20 |
able to detect | 20 |
compared with the | 20 |
the risk of | 20 |
are shown in | 20 |
in terms of | 20 |
avian influenza virus | 20 |
of a loop | 20 |
results showed that | 20 |
fold serial dilutions | 20 |
detection and quantification | 20 |
newcastle disease virus | 20 |
of viral rna | 20 |
was shown to | 20 |
due to its | 20 |
and detection of | 20 |
were used for | 20 |
without the need | 20 |
of the disease | 20 |
of sybr green | 20 |
there was no | 20 |
such as the | 20 |
on the other | 20 |
sensitivity of rt | 20 |
a dna polymerase | 19 |
reaction by loop | 19 |
a agarose gel | 19 |
lamp can be | 19 |
min in a | 19 |
followed by cycles | 19 |
the assay was | 19 |
of west nile | 19 |
lamp was performed | 19 |
from magnesium pyrophosphate | 19 |
was approved by | 19 |
from clinical samples | 19 |
pmol each of | 19 |
for rapid and | 19 |
could be used | 19 |
h n and | 19 |
copies per reaction | 19 |
water bath or | 19 |
method for detection | 19 |
sensitive and specific | 19 |
developed in this | 19 |
there is no | 19 |
the lamp primers | 19 |
the other hand | 19 |
to detect sars | 19 |
in the present | 19 |
hybridization chain reaction | 18 |
the reaction tube | 18 |
at a constant | 18 |
the united states | 18 |
lamp primers were | 18 |
assay was developed | 18 |
by cycles of | 18 |
a sensitivity of | 18 |
strand displacement activity | 18 |
of this assay | 18 |
detection of west | 18 |
autophagy and apoptosis | 18 |
reaction was performed | 18 |
linked immunosorbent assay | 18 |
the sensitivity and | 18 |
the sequences of | 18 |
indicating that the | 18 |
by a loop | 18 |
the amplified products | 18 |
reaction can be | 18 |
virus infection by | 18 |
turbidity derived from | 18 |
the mixture was | 18 |
lamp is a | 18 |
version posted may | 18 |
the results were | 18 |
to be a | 18 |
were subjected to | 18 |
in patients with | 18 |
were positive for | 18 |
with the rt | 18 |
nagamine et al | 18 |
primers targeting the | 18 |
the addition of | 18 |
of the sars | 18 |
on the target | 18 |
point of care | 18 |
isothermal dna amplification | 18 |
rna extracted from | 17 |
be used as | 17 |
has been developed | 17 |
study was approved | 17 |
forward inner primer | 17 |
magnesium pyrophosphate formation | 17 |
the authors declare | 17 |
reaction by turbidity | 17 |
in less than | 17 |
igm and igg | 17 |
dengue virus serotypes | 17 |
false positive results | 17 |
as a rapid | 17 |
assay can be | 17 |
a is the | 17 |
derived from magnesium | 17 |
respiratory syndrome virus | 17 |
by turbidity derived | 17 |
autophagy in the | 17 |
the study was | 17 |
viral nucleic acids | 17 |
lamp assay can | 17 |
sequence of the | 17 |
of herpes simplex | 17 |
hepatitis b virus | 17 |
under a is | 17 |
for the lamp | 17 |
is one of | 17 |
lamp assay has | 17 |
of this study | 17 |
indicated that the | 17 |
by using a | 17 |
of the rna | 17 |
the nucleic acid | 17 |
detection of influenza | 17 |
and stored at | 17 |
for infectious diseases | 17 |
of viral nucleic | 17 |
in sputum samples | 17 |
for use in | 17 |
the qlamp assay | 17 |
been used for | 17 |
with that of | 17 |
set of four | 17 |
displacement dna synthesis | 17 |
real time rt | 17 |
this work was | 17 |
orf a and | 17 |
molecular detection of | 17 |
ranging from to | 17 |
is shown in | 17 |
isothermal amplification rapid | 16 |
samples that were | 16 |
the results showed | 16 |
rapid and simple | 16 |
a color change | 16 |
pcr and real | 16 |
of a reverse | 16 |
lamp assay with | 16 |
sensitive and rapid | 16 |
amplification reaction by | 16 |
in the same | 16 |
of the most | 16 |
the samples were | 16 |
samples collected from | 16 |
were performed in | 16 |
higher than that | 16 |
the je virus | 16 |
in situ rt | 16 |
pcr and rt | 16 |
all of the | 16 |
as a template | 16 |
have also been | 16 |
time monitoring of | 16 |
the absence of | 16 |
for detecting sars | 16 |
for detection and | 16 |
regions of the | 16 |
lamp primer sets | 16 |
the viral rna | 16 |
amplification for the | 16 |
of lamp is | 16 |
respiratory syncytial virus | 16 |
using clinical samples | 16 |
be detected by | 16 |
diagnostic method for | 16 |
c for s | 16 |
influenza a virus | 16 |
backward inner primer | 16 |
of detection of | 16 |
by agarose gel | 16 |
isothermal amplification and | 16 |
color change from | 16 |
by notomi et | 16 |
the orf ab | 16 |
mycobacterium tuberculosis complex | 15 |
infectious bronchitis virus | 15 |
can be found | 15 |
were incubated at | 15 |
in our study | 15 |
the amplification reaction | 15 |
detection method for | 15 |
used as the | 15 |
pcr and lamp | 15 |
isothermal amplification assays | 15 |
temperature for min | 15 |
region of the | 15 |
been used to | 15 |
specific detection of | 15 |
cyp c g | 15 |
using the primer | 15 |
the microfluidic disc | 15 |
and n gene | 15 |
the reaction is | 15 |
the newly developed | 15 |
evaluation of the | 15 |
the treatment of | 15 |
thiessen et al | 15 |
has been widely | 15 |
differentiation of dengue | 15 |
has been applied | 15 |
carried out using | 15 |
of chikungunya virus | 15 |
and the results | 15 |
of f and | 15 |
in a total | 15 |
out in a | 15 |
in response to | 15 |
with high sensitivity | 15 |
lamp assays were | 15 |
in the detection | 15 |
most of the | 15 |
sequences of the | 15 |
human immunodeficiency virus | 15 |
demonstrated that the | 15 |
of lamp reaction | 15 |
world health organization | 15 |
lf and lb | 15 |
the majority of | 15 |
chain reaction for | 15 |
of the assays | 15 |
multisystemic wasting syndrome | 15 |
orf ab gene | 15 |
the amplification of | 15 |
of the ns | 14 |
based on a | 14 |
with high specificity | 14 |
the detection and | 14 |
primer explorer v | 14 |
rolling circle amplification | 14 |
with ethidium bromide | 14 |
dilutions of the | 14 |
the principle of | 14 |
the causative agent | 14 |
detection rate of | 14 |
of four primers | 14 |
the reaction time | 14 |
postweaning multisystemic wasting | 14 |
thermo fisher scientific | 14 |
was supported by | 14 |
nucleic acid extraction | 14 |
dna polymerase with | 14 |
in the acute | 14 |
combined with a | 14 |
as a result | 14 |
the most common | 14 |
in the future | 14 |
lamp and the | 14 |
be due to | 14 |
amplification of the | 14 |
study was to | 14 |
a novel coronavirus | 14 |
with a detection | 14 |
large numbers of | 14 |
to increase the | 14 |
a primer set | 14 |
with a ct | 14 |
the negative control | 14 |
of chik virus | 14 |
complementary to the | 14 |
and sensitivity of | 14 |
on a agarose | 14 |
because of its | 14 |
there is a | 14 |
t rna polymerase | 14 |
using reverse transcription | 14 |
of the primer | 14 |
the fluorescence signal | 14 |
two loop primers | 14 |
the ability to | 14 |
of the ap | 14 |
strand displacement dna | 14 |
regions on the | 14 |
in the study | 14 |
the concentration of | 14 |
that the lamp | 14 |
high specificity and | 14 |
have been reported | 14 |
based detection of | 14 |
integrated isothermal device | 14 |
isothermal amplification techniques | 14 |
viral rna was | 14 |
the conventional lamp | 14 |
results indicated that | 14 |
the concentrations of | 14 |
shows that the | 14 |
it can be | 14 |
with those of | 14 |
assay and the | 14 |
a mixture of | 14 |
detection of loop | 14 |
a positive result | 14 |
the detection rate | 14 |
of the two | 14 |
assay to detect | 13 |
to assess the | 13 |
to confirm the | 13 |
infection by a | 13 |
sequences in the | 13 |
molecular diagnosis of | 13 |
detection of japanese | 13 |
involved in the | 13 |
were analyzed by | 13 |
used as templates | 13 |
of the novel | 13 |
used to determine | 13 |
of a rapid | 13 |
pcr products were | 13 |
of the sample | 13 |
of mycobacterium ulcerans | 13 |
primer sets were | 13 |
virus by reverse | 13 |
transcriptase polymerase chain | 13 |
of lamp for | 13 |
of the f | 13 |
a standard curve | 13 |
pcr was performed | 13 |
conflict of interest | 13 |
were detected by | 13 |
influenza a and | 13 |
by the rt | 13 |
analysis of the | 13 |
and negative samples | 13 |
chen et al | 13 |
and application of | 13 |
and respiratory syndrome | 13 |
the advantages of | 13 |
in the case | 13 |
simple visual detection | 13 |
in a clinical | 13 |
of each primer | 13 |
the onset of | 13 |
samples were positive | 13 |
each of the | 13 |
the effect of | 13 |
detection of herpes | 13 |
the qpcr assay | 13 |
of porcine circovirus | 13 |
the opiph system | 13 |
was determined by | 13 |
of porcine parvovirus | 13 |
no conflict of | 13 |
for direct detection | 13 |
carried out at | 13 |
of dna amplification | 13 |
under uv light | 13 |
could be detected | 13 |
were compared with | 13 |
included in the | 13 |
intracellulare in sputum | 13 |
to be the | 13 |
lamp reactions were | 13 |
detection of infectious | 13 |
and the reaction | 13 |
of the amplification | 13 |
for min to | 13 |
amplification detection of | 13 |
was performed as | 13 |
the production of | 13 |
hbv lamp reaction | 13 |
two outer primers | 13 |
gel electrophoresis and | 13 |
the primer set | 13 |
of the viral | 13 |
the acute phase | 13 |
with or without | 13 |
amplification for direct | 13 |
genbank accession number | 13 |
for s and | 13 |
in accordance with | 13 |
by the naked | 13 |
intravenous alteplase infusion | 13 |
the amplifi cation | 13 |
detection of zika | 13 |
rna extraction kit | 12 |
were used in | 12 |
which can be | 12 |
clinical samples were | 12 |
reverse transcription polymerase | 12 |
were designed to | 12 |
h n virus | 12 |
transcription polymerase chain | 12 |
in the united | 12 |
polymerase with strand | 12 |
use of the | 12 |
present in the | 12 |
were able to | 12 |
mthfr c t | 12 |
causative agent of | 12 |
comparison of the | 12 |
shown to be | 12 |
it is not | 12 |
and simple visual | 12 |
the base apparatus | 12 |
that the detection | 12 |
assay for detecting | 12 |
samples were tested | 12 |
need for a | 12 |
is involved in | 12 |
in this review | 12 |
were designed using | 12 |
pcr assay for | 12 |
targeting the orf | 12 |
transcription loop mediated | 12 |
as described in | 12 |
as described previously | 12 |
reproductive and respiratory | 12 |
of the positive | 12 |
collected from patients | 12 |
products were detected | 12 |
between autophagy and | 12 |
of autophagy and | 12 |
this study is | 12 |
the end of | 12 |
dried blood spots | 12 |
laboratory diagnosis of | 12 |
acute phase of | 12 |
was able to | 12 |
the basis of | 12 |
molecular detection mix | 12 |
products of the | 12 |
the accuracy of | 12 |
dna or rna | 12 |
detection of covid | 12 |
the combination of | 12 |
and b primers | 12 |
was performed at | 12 |
evaluation of reverse | 12 |
of lamp primers | 12 |
targeting the n | 12 |
the replication of | 12 |
tool for the | 12 |
viral hemorrhagic septicaemia | 12 |
the accumulation of | 12 |
and visual detection | 12 |
dna and rna | 12 |
the process of | 12 |
lamp assay using | 12 |
use of a | 12 |
optimal reaction temperature | 12 |
there are several | 12 |
of a single | 12 |
well as the | 12 |
lamp primers targeting | 12 |
diagnosis of infectious | 12 |
porcine reproductive and | 12 |
been developed for | 12 |
the form of | 12 |
with a high | 12 |
performed using the | 12 |
positive in the | 12 |
on the basis | 12 |
mixture was incubated | 12 |
the polymerase chain | 12 |
the case of | 12 |
of bst dna | 12 |
viral rna in | 12 |
rna extraction protocol | 12 |
the orf a | 12 |
qpcr and rt | 12 |
of primers and | 12 |
colorimetric detection of | 12 |
the magnetic bead | 12 |
of hepatitis c | 12 |
is a novel | 12 |
lamp assay were | 12 |
fish and shellfish | 12 |
distinct regions on | 12 |
of target rna | 12 |
was incubated at | 12 |
diagnosis of fip | 12 |
was performed by | 11 |
of human herpesvirus | 11 |
authors declare no | 11 |
the reaction mix | 11 |
electrophoresis on a | 11 |
provided by the | 11 |
cov and mers | 11 |
members of the | 11 |
in real time | 11 |
for the development | 11 |
of six primers | 11 |
detection limits of | 11 |
lamp primer set | 11 |
and urine samples | 11 |
without rna extraction | 11 |
were designed by | 11 |
dna was extracted | 11 |
results in the | 11 |
as part of | 11 |
g for min | 11 |
o c for | 11 |
set of primers | 11 |
can be observed | 11 |
poon et al | 11 |
nucleic acids in | 11 |
supplementary table s | 11 |
assays have been | 11 |
real time pcr | 11 |
of clinical specimens | 11 |
loop primers loop | 11 |
be used in | 11 |
f fimbriae gene | 11 |
of middle east | 11 |
less than min | 11 |
of hpv dna | 11 |
of loop mediated | 11 |
and h n | 11 |
in agreement with | 11 |
rapid identification of | 11 |
to compare the | 11 |
used as template | 11 |
specificity of rt | 11 |
total volume of | 11 |
virus serotypes by | 11 |
and virus isolation | 11 |
health and disease | 11 |
viral nucleic acid | 11 |
the primers were | 11 |
copy number of | 11 |
has not been | 11 |
listed in table | 11 |
stained with ethidium | 11 |
of human papillomavirus | 11 |
detection of middle | 11 |
acid amplification tests | 11 |
with strand displacement | 11 |
sequences and simple | 11 |
incubated for min | 11 |
copy numbers of | 11 |
for rna extraction | 11 |
the reactions were | 11 |
strand displacement amplification | 11 |
compared with those | 11 |
by a real | 11 |
were stored at | 11 |
for the clinical | 11 |
monitoring of the | 11 |
were found to | 11 |
less sensitive than | 11 |
is needed to | 11 |
serotype specific rt | 11 |
the release of | 11 |
method to detect | 11 |
followed by a | 11 |
as a positive | 11 |
of loop primers | 11 |
ns serotype specific | 11 |
the coronavirus disease | 11 |
in this work | 11 |
the primer explorer | 11 |
as a diagnostic | 11 |
for the first | 11 |
is the first | 11 |
the lowest detection | 11 |
in a water | 11 |
preprint in the | 11 |
the high sensitivity | 11 |
using a reverse | 11 |
in contrast to | 11 |
transmissible gastroenteritis coronavirus | 11 |
obtained from the | 11 |
the specificity and | 11 |
been developed to | 11 |
acid amplification method | 11 |
of patients with | 11 |
detect viral rna | 11 |
been applied to | 11 |
was used in | 11 |
conserved region of | 11 |
a positive control | 11 |
for disease control | 11 |
results can be | 11 |
dna amplification method | 11 |
lamp assay developed | 11 |
of a new | 11 |
amplified products were | 11 |
have to be | 11 |
isothermal amplification a | 11 |
lamp assay showed | 11 |
reaction time of | 11 |
rapid and reliable | 11 |
the same as | 11 |
with and without | 11 |
c g g | 11 |
to detect viral | 11 |
the rna extraction | 11 |
reaction for the | 11 |
the novel rt | 11 |
the identification of | 11 |
for influenza a | 11 |
version posted august | 11 |
the amount of | 11 |
the design of | 11 |
to test the | 11 |
in the copyright | 11 |
reverse transcription and | 11 |
method has been | 11 |
influenza virus infection | 11 |
an increase in | 11 |
new generation of | 11 |
the target gene | 11 |
diagnosis of h | 11 |
min and then | 11 |
a new generation | 11 |
n gene of | 11 |
such as a | 11 |
a range of | 11 |
in developing countries | 11 |
dna extraction and | 11 |
when the template | 10 |
supported by the | 10 |
the intensity of | 10 |
to achieve a | 10 |
amplification for detection | 10 |
to the results | 10 |
in case of | 10 |
detection of nucleic | 10 |
time pcr for | 10 |
use of this | 10 |
sensitivity of lamp | 10 |
been found to | 10 |
was to develop | 10 |
rapid molecular detection | 10 |
to overcome the | 10 |
the standard curve | 10 |
prevention and control | 10 |
samples from patients | 10 |
the colorimetric rt | 10 |
rapid and specific | 10 |
detection of pathogens | 10 |
results indicate that | 10 |
lamp assay in | 10 |
found in the | 10 |
a panel of | 10 |
in the clinical | 10 |
the novel coronavirus | 10 |
due to their | 10 |
was detected in | 10 |
isothermal master mix | 10 |
of dna loop | 10 |
classical swine fever | 10 |
performed using a | 10 |
of isothermal amplification | 10 |
dried blood spot | 10 |
this method has | 10 |
virol methods doi | 10 |
viral load in | 10 |
been used in | 10 |
application of the | 10 |
to be used | 10 |
gene sequences and | 10 |
may not be | 10 |
h and h | 10 |
was mixed with | 10 |
thought to be | 10 |
out of the | 10 |
sets of primers | 10 |
as that of | 10 |
human genomic dna | 10 |
the virus in | 10 |
c for sec | 10 |
two sets of | 10 |
less than h | 10 |
a template for | 10 |
to detect a | 10 |
a thermal cycler | 10 |
virus rapid detection | 10 |
be carried out | 10 |
of lamp was | 10 |
in clinical specimens | 10 |
in combination with | 10 |
swine transmissible gastroenteritis | 10 |
can be applied | 10 |
detection and typing | 10 |
for h n | 10 |
dependent rna polymerase | 10 |
of newcastle disease | 10 |
and identification of | 10 |
it is possible | 10 |
in the absence | 10 |
tomita et al | 10 |
and specific detection | 10 |
and compared with | 10 |
lamp for sars | 10 |
was eluted in | 10 |
amplification assays for | 10 |
mouth disease virus | 10 |
serve as a | 10 |
of the dna | 10 |
in comparison to | 10 |
the sample was | 10 |
of the first | 10 |
isothermal amplification detection | 10 |
lamp method has | 10 |
other respiratory viruses | 10 |
of turkey coronavirus | 10 |
of viral hemorrhagic | 10 |
detection of nvs | 10 |
in table s | 10 |
porcine epidemic diarrhea | 10 |
a member of | 10 |
of the tube | 10 |
agreement with the | 10 |
and typing of | 10 |
work was supported | 10 |
epidermal growth factor | 10 |
was designed to | 10 |
would like to | 10 |
lamp reaction for | 10 |
lateral flow dipstick | 10 |
reactions were carried | 10 |
yellow head virus | 10 |
is able to | 10 |
diagnosis of sars | 10 |
the color change | 10 |
lamp reaction is | 10 |
determine the optimal | 10 |
spread of the | 10 |
at the end | 10 |
the copy number | 10 |
lamp for the | 10 |
hemorrhagic septicaemia virus | 10 |
is a simple | 10 |
to improve the | 10 |
method for detecting | 10 |
and backward inner | 10 |
primers for the | 10 |
and n genes | 10 |
by lamp and | 10 |
depending on the | 10 |
of the detection | 10 |
gargle lavage samples | 10 |
a limit of | 10 |
results were compared | 10 |
development of reverse | 10 |
required for the | 10 |
enterotoxigenic escherichia coli | 10 |
need to be | 10 |
h n avian | 10 |
gene and n | 10 |
of feline coronavirus | 10 |
have been used | 10 |
rna can be | 10 |
of influenza viruses | 10 |
ethics committee of | 10 |
j virol methods | 10 |
assay has been | 10 |
using the rt | 10 |
positive samples were | 10 |
of magnesium pyrophosphate | 10 |
with other respiratory | 10 |
of target dna | 10 |
the activity of | 10 |
growth factor receptor | 10 |
of gene sequences | 10 |
the viral genome | 10 |
of h n | 10 |
a total volume | 10 |
methods for the | 10 |
acid amplification test | 10 |
sensitive than the | 10 |
been reported that | 10 |
detection of products | 10 |
that of rt | 9 |
were heated at | 9 |
a novel nucleic | 9 |
virus in the | 9 |
in the form | 9 |
amplification products were | 9 |
application of a | 9 |
influenza a h | 9 |
none of the | 9 |
were included in | 9 |
detection of dengue | 9 |
in recent years | 9 |
final volume of | 9 |
suitable for the | 9 |
in situ hybridization | 9 |
amplification of nucleic | 9 |
did not cross | 9 |
some of the | 9 |
different concentrations of | 9 |
hydroxynaphthol blue dye | 9 |
with the lamp | 9 |
false positives and | 9 |
dna polymerase and | 9 |
set of six | 9 |
is characterized by | 9 |
by gel electrophoresis | 9 |
a specificity of | 9 |
lamp method for | 9 |
gold standard for | 9 |
results of rt | 9 |
quantifi cation of | 9 |
mycobacterium ulcerans infection | 9 |
at the point | 9 |
detected by the | 9 |
specific for the | 9 |
of cats with | 9 |
reactions were performed | 9 |
to the qpcr | 9 |
with hydroxynaphthol blue | 9 |
rna polymerase promoter | 9 |
carotid artery stenting | 9 |
optical detection unit | 9 |
of feline infectious | 9 |
the results indicated | 9 |
liu et al | 9 |
used for rt | 9 |
n gene and | 9 |
the amplification efficiency | 9 |
we used the | 9 |
primers f and | 9 |
consists of a | 9 |
use in the | 9 |
of rna from | 9 |
a series of | 9 |
of each serotype | 9 |
lamp assay to | 9 |
the conventional rt | 9 |
compared to rt | 9 |
primer set a | 9 |
lateral flow assay | 9 |
the ability of | 9 |
epidemic diarrhea virus | 9 |
specific and sensitive | 9 |
in the early | 9 |
dilution series of | 9 |
of autophagy by | 9 |
of the amplified | 9 |
a single reaction | 9 |
was confirmed by | 9 |
a reverse transcriptase | 9 |
the viral load | 9 |
the best of | 9 |
nucleic acids and | 9 |
patients infected with | 9 |
development of the | 9 |
development of an | 9 |
assay developed in | 9 |
an isothermal amplification | 9 |
a lamp assay | 9 |
compared with conventional | 9 |
ibv or ndv | 9 |
rate of the | 9 |
assay for sars | 9 |
n avian influenza | 9 |
it is also | 9 |
applied to the | 9 |
the rate of | 9 |
of ibv and | 9 |
we evaluated the | 9 |
and control of | 9 |
using a simple | 9 |
the time required | 9 |
repeats of the | 9 |
considered to be | 9 |
in clinical diagnosis | 9 |
pharyngeal swab specimens | 9 |
of escherichia coli | 9 |
room temperature for | 9 |
to identify the | 9 |
the developed rt | 9 |
is the most | 9 |
and reverse transcription | 9 |
disease control and | 9 |
a fast and | 9 |
agarose gel and | 9 |
the assay is | 9 |
total rna was | 9 |
chain reaction and | 9 |
the severe acute | 9 |
using the lamp | 9 |
was assessed by | 9 |
diagnostic methods for | 9 |
to the target | 9 |
been widely used | 9 |
is possible to | 9 |
and bip primers | 9 |
of the method | 9 |
the clinical samples | 9 |
of the test | 9 |
of our knowledge | 9 |
lamp was carried | 9 |
method for covid | 9 |
was extracted using | 9 |
highly specific for | 9 |
a novel reverse | 9 |
a h n | 9 |
is thought to | 9 |
by adding a | 9 |
in the public | 9 |
body cavity effusions | 9 |
reverse transcriptase polymerase | 9 |
high sensitivity of | 9 |
novel reverse transcription | 9 |
n and h | 9 |
the lamp reactions | 9 |
amplification combined with | 9 |
is a major | 9 |
extracted from the | 9 |
as described above | 9 |
diagnostic test for | 9 |
to reduce the | 9 |
a single tube | 9 |
lamp assays for | 9 |
could be a | 9 |
best of our | 9 |
the reaction mixtures | 9 |
of copies of | 9 |
the feasibility of | 9 |
for the identification | 9 |
b lamp assay | 9 |
is required to | 9 |
from red to | 9 |
be found in | 9 |
to the best | 9 |
time pcr system | 9 |
assay was performed | 9 |
the lamp assays | 9 |
of the je | 9 |
influenza a b | 9 |
infected with wild | 9 |
aldh glu lys | 9 |
swine fever virus | 9 |
has been demonstrated | 9 |
were negative by | 9 |
a wide range | 9 |
the advantage of | 9 |
in the target | 9 |
amplified dna products | 9 |
with the same | 9 |
pairs of primers | 9 |
performed as described | 9 |
has a high | 9 |
complementary sequence of | 9 |
and lateral flow | 9 |
is that the | 9 |
assays for the | 9 |
the result of | 9 |
dna by loop | 9 |
detection of salmonella | 9 |
the dna polymerase | 9 |
c for h | 9 |
may be due | 9 |
the possibility of | 9 |
and igg antibodies | 9 |
the amplified dna | 9 |
of a real | 9 |
was purchased from | 9 |
react with other | 9 |
isothermal amplification development | 9 |
the lack of | 9 |
wide range of | 9 |
compared to conventional | 9 |
well as in | 9 |
pcr assay was | 9 |
lamp detection of | 9 |
determined to be | 9 |
pair of primers | 9 |
e gene and | 9 |
resulted in a | 9 |
centrifugal microfluidic system | 9 |
and it is | 9 |
use of lamp | 9 |
zika virus in | 9 |
and cyp c | 9 |
lamp assay on | 9 |
the disposable micro | 9 |
amplifi cation method | 8 |
cell lung cancer | 8 |
time nucleic acid | 8 |
amplification technologies for | 8 |
the potential of | 8 |
to obtain a | 8 |
than the rt | 8 |
lamp method is | 8 |
declare no conflict | 8 |
primers were used | 8 |
a single step | 8 |
amplification can be | 8 |
for the presence | 8 |
used to design | 8 |
efficacy and safety | 8 |
prior to the | 8 |
each sample was | 8 |
in lamp reactions | 8 |
reactions can be | 8 |
hais due to | 8 |
nasopharyngeal swab samples | 8 |
acid amplification technologies | 8 |
to prevent the | 8 |
i influenza a | 8 |
detection of lamp | 8 |
rapid and visual | 8 |
development and application | 8 |
primer sets for | 8 |
part of the | 8 |
activity of the | 8 |
the reaction and | 8 |
difference between the | 8 |
is required for | 8 |
components of the | 8 |
lamp assay had | 8 |
needs to be | 8 |
as the template | 8 |
to the high | 8 |
and nucleic acid | 8 |
reaction was carried | 8 |
newly developed rt | 8 |
were designed based | 8 |
genes of sars | 8 |
products were electrophoresed | 8 |
of egfr mutations | 8 |
ml of the | 8 |
acute ischemic stroke | 8 |
the course of | 8 |
of human influenza | 8 |
negative samples were | 8 |
to determine if | 8 |
c t and | 8 |
serotypes by a | 8 |
serum and urine | 8 |
a temperature of | 8 |
inverted repeats of | 8 |
mechanism of action | 8 |
the utility of | 8 |
a fluorescent dye | 8 |
indicates that the | 8 |
in whole blood | 8 |
there is an | 8 |
mutations in the | 8 |
and the other | 8 |
the first report | 8 |
lamp is more | 8 |
time pcr assay | 8 |
are presented in | 8 |
n genes of | 8 |
the efficiency of | 8 |
those of the | 8 |
caused by the | 8 |
no need for | 8 |
a magnetic bead | 8 |
and lamp assays | 8 |
the hbv lamp | 8 |
of this technique | 8 |
no competing interests | 8 |
control and prevention | 8 |
for quantification of | 8 |
products were analyzed | 8 |
mm each of | 8 |
cdc real time | 8 |
designed based on | 8 |
of less than | 8 |
coronavirus rna in | 8 |
and the sensitivity | 8 |
was not peer | 8 |
function of the | 8 |
isolated from pharyngeal | 8 |
virus infection in | 8 |
of the infection | 8 |
using the same | 8 |
efficiency of the | 8 |
a lateral flow | 8 |
grape powdery mildew | 8 |
for pdm h | 8 |
with the results | 8 |
with clinical samples | 8 |
for reverse transcription | 8 |
time required for | 8 |
of dna template | 8 |
the positive reaction | 8 |
the conventional kit | 8 |
purified dna extracts | 8 |
the s protein | 8 |
hong et al | 8 |
of sry gene | 8 |
tissues and feces | 8 |
f f f | 8 |
phase of infection | 8 |
amplification method rapid | 8 |
we used a | 8 |
the agarose gel | 8 |
acid amplification methods | 8 |
the products of | 8 |
green i was | 8 |
limit of rt | 8 |
member of the | 8 |
is suitable for | 8 |
simultaneous detection of | 8 |
this is the | 8 |
a final volume | 8 |
were visualized by | 8 |
the primer sets | 8 |
with lateral flow | 8 |
of hbv dna | 8 |
of the clinical | 8 |
region of hpv | 8 |
transcription loopmediated isothermal | 8 |
products from the | 8 |
were positive by | 8 |
is similar to | 8 |
highly sensitive and | 8 |
sensitivity of this | 8 |
to create a | 8 |
as the target | 8 |
the reaction can | 8 |
b and b | 8 |
be detected in | 8 |
of reverse transcriptase | 8 |
for the amplification | 8 |
assay detection results | 8 |
the extracted rna | 8 |
formation of a | 8 |
the two assays | 8 |
the lamp products | 8 |
with other viruses | 8 |
a new coronavirus | 8 |
the qiaamp viral | 8 |
and the amplification | 8 |
by means of | 8 |
the gold standard | 8 |
than conventional rt | 8 |
a pair of | 8 |
of dna rapid | 8 |
novel nucleic acid | 8 |
forward and reverse | 8 |
we decided to | 8 |
was subjected to | 8 |
the point of | 8 |
caused by a | 8 |
ct values for | 8 |
the primers used | 8 |
diagnosis for covid | 8 |
the levels of | 8 |
rna was eluted | 8 |
due to ics | 8 |
were detected in | 8 |
detection of hepatitis | 8 |
are needed to | 8 |
have not been | 8 |
in the treatment | 8 |
a combination of | 8 |
which is a | 8 |
on ice for | 8 |
of dengue viruses | 8 |
reaction mixture was | 8 |
time to positivity | 8 |
assay for covid | 8 |
c and d | 8 |
samples obtained from | 8 |
specificity of lamp | 8 |
detection of m | 8 |
and on the | 8 |
the potential to | 8 |
test for the | 8 |
and aldh glu | 8 |
of the pcr | 8 |
positive predictive value | 8 |
all primers were | 8 |
the amplified product | 8 |
perspectives in clinical | 8 |
virus dna by | 8 |
all samples were | 8 |
and does not | 8 |
of l of | 8 |
test the specificity | 8 |
same as that | 8 |
was performed with | 8 |
development of loop | 8 |
amplification assay development | 8 |
of denv infection | 8 |
the rna was | 8 |
with the naked | 8 |
of the covid | 8 |
primers that recognize | 8 |
the sequence of | 8 |
serial dilution of | 8 |
the outbreak of | 8 |
human influenza a | 8 |
that have been | 8 |
higher sensitivity than | 8 |
methods for detection | 8 |
in the laboratory | 8 |
early diagnosis of | 8 |
detection of turkey | 8 |
that were negative | 8 |
lamp assay that | 8 |
on the principle | 8 |
teoh et al | 8 |
direct nucleic acid | 8 |
of lamp products | 8 |
swabs and sputum | 8 |
of coronavirus disease | 8 |
because it is | 8 |
the need of | 8 |
of hpv type | 8 |
the level of | 8 |
of the turbidity | 8 |
when the reaction | 8 |
qiaamp viral rna | 8 |
of primers were | 8 |
without loop primers | 8 |
the products were | 8 |
accordance with the | 8 |
to ensure that | 8 |
amv reverse transcriptase | 8 |
displacement activity and | 8 |
shown to induce | 8 |
assay development and | 8 |
were added to | 8 |
quantitative detection of | 8 |
most widely used | 8 |
is associated with | 8 |
e region of | 8 |
the detection limits | 8 |
the f and | 8 |
and conventional rt | 8 |
of fip and | 8 |
that the assay | 8 |
color of the | 8 |
a commercial rt | 8 |
system for rapid | 8 |
eight distinct regions | 8 |
there was a | 8 |
during the acute | 8 |
of acute gastroenteritis | 8 |
after the reaction | 8 |
lamp method was | 8 |
was developed for | 8 |
authors declare that | 8 |
the reliability of | 8 |
to validate the | 8 |
were synthesized by | 8 |
from to copies | 8 |
by the lamp | 8 |
a positive signal | 8 |
lamp could be | 8 |
red to yellow | 8 |
designed using the | 8 |
b and c | 8 |
corresponding to the | 8 |
the diagnostic laboratory | 8 |
was less than | 8 |
assay is a | 8 |
directly from heat | 7 |
the immune system | 7 |
rna was used | 7 |
with pulmonary adenocarcinoma | 7 |
turkey coronavirus rna | 7 |
as evidenced by | 7 |
is compatible with | 7 |
detection of fcov | 7 |
the mechanism of | 7 |
it is important | 7 |
the ec nv | 7 |
a concentration of | 7 |
the primers and | 7 |
the rapid diagnosis | 7 |
virus isolation and | 7 |
between the two | 7 |
to the standard | 7 |
times more sensitive | 7 |
indicate that the | 7 |
activity of dna | 7 |
for molecular diagnostics | 7 |
study showed that | 7 |
kageyama et al | 7 |
pcr and qrt | 7 |
roles of autophagy | 7 |
copies reaction denv | 7 |
and has been | 7 |
the isothermal mastermix | 7 |
acid amplification using | 7 |
from pharyngeal swabs | 7 |
simple and sensitive | 7 |
isothermal amplification date | 7 |
amplification in the | 7 |
fold more sensitive | 7 |
also known as | 7 |
the b c | 7 |
negative and positive | 7 |
widely used in | 7 |
under field conditions | 7 |
for clinical diagnosis | 7 |
rna in the | 7 |
the reaction temperature | 7 |
mgcl and dntps | 7 |
was found in | 7 |
f and f | 7 |
samples can be | 7 |
amplification was performed | 7 |
be suitable for | 7 |
is highly specific | 7 |
each of f | 7 |
clinical samples was | 7 |
the bst dna | 7 |
been reported in | 7 |
detection limit was | 7 |
of cyp c | 7 |
of the results | 7 |
two pairs of | 7 |
derived from the | 7 |
loop primers were | 7 |
under a author | 7 |
our study was | 7 |
time of min | 7 |
to facilitate the | 7 |
to be involved | 7 |
used for detection | 7 |
to minimize the | 7 |
four sets of | 7 |
of the manuscript | 7 |
great potential for | 7 |
applied to detect | 7 |
sequence of f | 7 |
version of the | 7 |
the lamp reagents | 7 |
lamp assays have | 7 |
was developed by | 7 |
of coronavirus sars | 7 |
details of the | 7 |
the online version | 7 |
used to treat | 7 |
virus genome by | 7 |
isothermal amplification as | 7 |
and centrifuged at | 7 |
ethidium bromide and | 7 |
samples isolated from | 7 |
chikungunya virus by | 7 |
validation of a | 7 |
rna extraction and | 7 |
by the use | 7 |
found that the | 7 |
of lamp assay | 7 |
pcr for the | 7 |
of listeria monocytogenes | 7 |
to prevent cross | 7 |
the acute stage | 7 |
were compared to | 7 |
the ccc pipeline | 7 |
characterization of the | 7 |
of conventional rt | 7 |
of dna accelerated | 7 |
lamp to detect | 7 |
a colorimetric rt | 7 |
between f and | 7 |
by targeting the | 7 |
pcr is the | 7 |
the most important | 7 |
used to identify | 7 |
as templates for | 7 |
by the ethics | 7 |
of the y | 7 |
conditions of the | 7 |
was developed to | 7 |
ll of the | 7 |
sense sequence of | 7 |
accuracy of the | 7 |
a serial dilution | 7 |
white precipitate of | 7 |
developed a rapid | 7 |
is that it | 7 |
and two loop | 7 |
methods have been | 7 |
of f fimbriae | 7 |
final concentration of | 7 |
onset of symptoms | 7 |
sequence based amplification | 7 |
use of rt | 7 |
was obtained from | 7 |
pot visual rt | 7 |
amplification efficiency of | 7 |
samples with ct | 7 |
after the onset | 7 |
nile virus rapid | 7 |
assays for detection | 7 |
constant temperature of | 7 |
to detect rna | 7 |
rna samples isolated | 7 |
of the genus | 7 |
detection of newcastle | 7 |
assay may be | 7 |
dna was detected | 7 |
samples had been | 7 |
is known to | 7 |
and in the | 7 |
science foundation of | 7 |
in conjunction with | 7 |
dependent isothermal dna | 7 |
using colorimetric lamp | 7 |
has been found | 7 |
in peripheral blood | 7 |
of the plasmid | 7 |
stages of the | 7 |
mean detection rates | 7 |
of whole blood | 7 |
applied for the | 7 |
the recent outbreak | 7 |
the rna standard | 7 |
of each of | 7 |
the optimal temperature | 7 |
of lamp and | 7 |
higher than the | 7 |
capable of detecting | 7 |
was the same | 7 |
reaction mixture contained | 7 |
the same strand | 7 |
associated with a | 7 |
are associated with | 7 |
of rna template | 7 |
sensitivity of detection | 7 |
in upper respiratory | 7 |
a final concentration | 7 |
fold higher than | 7 |
similar to that | 7 |
lamp for rapid | 7 |
increase in the | 7 |
the reaction tubes | 7 |
the inner primers | 7 |
the cdc real | 7 |
gene copy number | 7 |
indicating that autophagy | 7 |
was stored at | 7 |
carried out by | 7 |
were suspended in | 7 |
gene amplification technique | 7 |
covert mortality disease | 7 |
lamp assay may | 7 |
compared with that | 7 |
the time of | 7 |
resulting in the | 7 |
can lead to | 7 |
diagnosis of coronavirus | 7 |
products were visualized | 7 |
in cell culture | 7 |
yang et al | 7 |
was performed on | 7 |
proliferative kidney disease | 7 |
the control group | 7 |
the most widely | 7 |
a comparison of | 7 |
that the sensitivity | 7 |
in tissues and | 7 |
generation of innovative | 7 |
dna accelerated reaction | 7 |
are listed in | 7 |
h avian influenza | 7 |
detection of chikungunya | 7 |
role in the | 7 |
in a pcr | 7 |
not suitable for | 7 |
the value of | 7 |
innovative gene amplification | 7 |
using the qiaamp | 7 |
an asymptomatic carrier | 7 |
method rapid detection | 7 |
diagnosis and treatment | 7 |
the activation of | 7 |
rna in tissues | 7 |
human herpesvirus dna | 7 |
for the n | 7 |
detection of rna | 7 |
coronavirus using a | 7 |
detection of potato | 7 |
by polymerase chain | 7 |
molecular test for | 7 |
can be conducted | 7 |
positive results in | 7 |
and the conventional | 7 |
to carry out | 7 |
on the gel | 7 |
of lamp in | 7 |
nasopharyngeal swabs from | 7 |
pcr was carried | 7 |
by electrophoresis on | 7 |
reported in the | 7 |
the f c | 7 |
to analyze the | 7 |
due to a | 7 |
of isothermal nucleic | 7 |
clinical diagnostic laboratory | 7 |
acid sequence based | 7 |
could not be | 7 |
blood samples from | 7 |
regulation of autophagy | 7 |
in the initial | 7 |
to provide a | 7 |
can be easily | 7 |
lamp reaction mixtures | 7 |
of salmonella enterica | 7 |
of each sample | 7 |
we report a | 7 |
to amplify the | 7 |
reverse transcription loopmediated | 7 |
addition to the | 7 |
by a reverse | 7 |
between the rt | 7 |
lamp primer design | 7 |
lamp in a | 7 |
by the loop | 7 |
detection of hpv | 7 |
were mixed with | 7 |
the growing season | 7 |
immunodeficiency virus type | 7 |
resulting in a | 7 |
patients with pulmonary | 7 |
an independent cohort | 7 |
six distinct sequences | 7 |
when detecting ibv | 7 |
gel electrophoresis or | 7 |
the clinical diagnosis | 7 |
present study was | 7 |
been reported to | 7 |
surface plasmon resonance | 7 |
was positive in | 7 |
pcr detection of | 7 |
the positive control | 7 |
of this article | 7 |
concentrations of primers | 7 |
negative predictive value | 7 |
belong to the | 7 |
can be visualized | 7 |
high degree of | 7 |
of buruli ulcer | 7 |
amplification rapid detection | 7 |
has been described | 7 |
the type of | 7 |
of mthfr c | 7 |
were designed from | 7 |
dna extracted from | 7 |
enzymatic amplification of | 7 |
in the development | 7 |
following intravenous alteplase | 7 |
reaction mixtures were | 7 |
lamp amplifi cation | 7 |
positive by rt | 7 |
like to thank | 7 |
detection based on | 7 |
amplification development of | 7 |
isothermal amplification combined | 7 |
primer set for | 7 |
to examine the | 7 |
directly from clinical | 7 |
forward loop primer | 7 |
lamp and prv | 7 |
be applied for | 7 |
method for infectious | 7 |
detecting ibv and | 7 |
the plate was | 7 |
lamp and conventional | 7 |
of the fip | 7 |
were confirmed by | 7 |
sequence of b | 7 |
and there is | 7 |
has the advantage | 7 |
in the current | 7 |
at the same | 7 |
the master mix | 7 |
lamp had a | 7 |
as a negative | 7 |
simple method for | 7 |
detection of feline | 7 |
the target snp | 7 |
at r min | 7 |
the establishment of | 7 |
early stages of | 7 |
and the lamp | 7 |
on the lamp | 7 |
committee of the | 7 |
clinical application of | 7 |
is essential for | 7 |
kit for the | 7 |
wang et al | 7 |
automated centrifugal microfluidic | 7 |
method can be | 7 |
compared to pcr | 7 |
the prv ge | 7 |
reactivity with other | 7 |
for cyp c | 7 |
sets were designed | 7 |
of the study | 7 |
this study showed | 7 |
products can be | 7 |
highly sensitive detection | 7 |
direct pcr amplification | 7 |
the y chromosome | 7 |
effective diagnostic method | 7 |
the template input | 7 |
dna amplification and | 7 |
alere i influenza | 7 |
f c and | 7 |
and west nile | 7 |
that it is | 7 |
clinical samples and | 7 |
ms and rf | 7 |
the ethics committee | 7 |
there were no | 7 |
of innovative gene | 7 |
equipment such as | 7 |
a author funder | 7 |
subjected to rt | 7 |
syndrome coronavirus rapid | 7 |
performed in the | 7 |
all of these | 7 |
in a reaction | 7 |
of the newly | 7 |
the genome of | 7 |
amount of dna | 7 |
of the denv | 7 |
correlation between the | 7 |
assay using a | 7 |
these samples were | 7 |
summarized in table | 7 |
a negative control | 7 |
amplification as a | 7 |
reaction volume containing | 6 |
rna using colorimetric | 6 |
diagnosis of dengue | 6 |
gel electrophoresis was | 6 |
by the national | 6 |
for virus detection | 6 |
viral rna mini | 6 |
detection of tomato | 6 |
to design the | 6 |
dna from the | 6 |
specificity for the | 6 |
swab samples were | 6 |
were defined as | 6 |
can provide a | 6 |
based rna extraction | 6 |
primers fip and | 6 |
foundation of china | 6 |
anneals to the | 6 |
our previous study | 6 |
techniques have been | 6 |
of dna with | 6 |
was not detected | 6 |
amplification of rna | 6 |
autophagy has been | 6 |
a viruses by | 6 |
detected in samples | 6 |
it suitable for | 6 |
depicted in figure | 6 |
the lysis buffer | 6 |
of c for | 6 |
patients with pneumonia | 6 |
size of the | 6 |
distilled water and | 6 |
have indicated that | 6 |
virus by real | 6 |
was less sensitive | 6 |
well plate was | 6 |
detection of swine | 6 |
gunimaladevi et al | 6 |
x primer mix | 6 |
primers used for | 6 |
the ns gene | 6 |
detection in the | 6 |
essential for the | 6 |
and reverse primers | 6 |
the primers are | 6 |
n and n | 6 |
advantage of the | 6 |
pcr as a | 6 |
institutional review board | 6 |
studies have shown | 6 |
qlamp assay detection | 6 |
lower than that | 6 |
values for the | 6 |
loops formed by | 6 |
creative commons attribution | 6 |
time reverse transcriptase | 6 |
can be an | 6 |
reaction volume of | 6 |
times higher than | 6 |
the restriction enzyme | 6 |
the established rt | 6 |
been developed and | 6 |
can be carried | 6 |
as a means | 6 |
number of rna | 6 |
x master mix | 6 |
sets of lamp | 6 |
its ability to | 6 |
carried out with | 6 |
and sputum samples | 6 |
used to amplify | 6 |
rna extraction kits | 6 |
multiple displacement amplification | 6 |
described in section | 6 |
developed for detection | 6 |
including two outer | 6 |
dna amplification in | 6 |
pacific white shrimp | 6 |
step reverse transcription | 6 |
characteristics of the | 6 |
positive reaction was | 6 |
the cap of | 6 |
chronic hepatitis b | 6 |
negative by rt | 6 |
declare that they | 6 |
both the rt | 6 |
reverse transcriptase loop | 6 |
colorimetric lamp assay | 6 |
suggested that the | 6 |
also be used | 6 |
assay does not | 6 |
of infection and | 6 |
detection of dna | 6 |
higher sensitivity and | 6 |
be observed by | 6 |
and the prv | 6 |
concentration of the | 6 |
independent cohort of | 6 |
in health and | 6 |
simple water bath | 6 |
and had a | 6 |
suitable for use | 6 |
science and technology | 6 |
in the cap | 6 |
amplification reaction for | 6 |
encephalitis virus by | 6 |
peripheral blood mononuclear | 6 |
sequence analysis of | 6 |
of copies reaction | 6 |
is expected to | 6 |
revealed that the | 6 |
bronchoalveolar lavage fluid | 6 |
were negative for | 6 |
lamp assay could | 6 |
bhadra et al | 6 |
other groups have | 6 |
the pi k | 6 |
it does not | 6 |
warmstart colorimetric lamp | 6 |
of dna polymerase | 6 |
assay in the | 6 |
assay could be | 6 |
relies on the | 6 |
this article can | 6 |
we would like | 6 |
the impact of | 6 |
refers to the | 6 |
in blood samples | 6 |
plasma and serum | 6 |
viral rna detection | 6 |
of respiratory syncytial | 6 |
be involved in | 6 |
positive for h | 6 |
performed on the | 6 |
has been successfully | 6 |
a critical review | 6 |
designed from the | 6 |
informed consent was | 6 |
with a low | 6 |
developed for the | 6 |
isothermal amplification kit | 6 |
in line with | 6 |
and serotyping of | 6 |
is more sensitive | 6 |
with ml of | 6 |
internal carotid artery | 6 |
as low as | 6 |
fl uorescent dye | 6 |
developed to detect | 6 |
targeting the e | 6 |
by visual inspection | 6 |
is important to | 6 |
small sample size | 6 |
an outbreak of | 6 |
lamp reaction products | 6 |
each of primers | 6 |
it shows that | 6 |
of loopmediated isothermal | 6 |
for snp detection | 6 |
autophagy in cancer | 6 |
of mgcl and | 6 |
relative to the | 6 |
with a sensitivity | 6 |
autophagy is a | 6 |
to have a | 6 |
using a portable | 6 |
in a biosafety | 6 |
used as an | 6 |
limit of copies | 6 |
was applied to | 6 |
nucleic acids with | 6 |
when compared to | 6 |
reliable detection of | 6 |
in the assay | 6 |
amplification using ph | 6 |
was measured by | 6 |
also been used | 6 |
strand displacement reaction | 6 |
of the real | 6 |
may be used | 6 |
feces by reverse | 6 |
and highly sensitive | 6 |
on the sample | 6 |
amplification test for | 6 |
detection of various | 6 |
for lamp and | 6 |
negative diagnosis for | 6 |
confirmed by sequencing | 6 |
inhibition of autophagy | 6 |
the world health | 6 |
and quantifi cation | 6 |
can be read | 6 |
and did not | 6 |
by centrifugation at | 6 |
reliability of the | 6 |
for amplification of | 6 |
emerging infectious diseases | 6 |
assay should be | 6 |
detection of a | 6 |
the control line | 6 |
with ct values | 6 |
been applied for | 6 |
molecular diagnostic test | 6 |
reverse transcription step | 6 |
the mean detection | 6 |
sample to result | 6 |
is an isothermal | 6 |
a novel method | 6 |
the ap pathway | 6 |
by a novel | 6 |
autophagic cell death | 6 |
other related viruses | 6 |
assay and lamp | 6 |
of nested rt | 6 |
in the growing | 6 |
dna was eluted | 6 |
of the direct | 6 |
diagnostic potential of | 6 |
the aim of | 6 |
detection of african | 6 |
viral load of | 6 |
with the use | 6 |
based on these | 6 |
culture medium and | 6 |
lamp method can | 6 |
test for sars | 6 |
that the primers | 6 |
for sec and | 6 |
and copies of | 6 |
the cyp c | 6 |
sample preparation and | 6 |
appears to be | 6 |
a valuable tool | 6 |
the host cell | 6 |
mediated isothermal amplificatio | 6 |
on the same | 6 |
which results in | 6 |
from the rt | 6 |
copies of is | 6 |
be adapted to | 6 |
was established by | 6 |
a method for | 6 |
methods for detecting | 6 |
in vitro and | 6 |
of the beads | 6 |
the primer sequences | 6 |
targeting orf a | 6 |
up to fold | 6 |
slit lamp shield | 6 |
visualization of the | 6 |
crispr cas a | 6 |
of the mixture | 6 |
positive for sars | 6 |
to inhibit autophagy | 6 |
without dna extraction | 6 |
that of pcr | 6 |
early stage of | 6 |
the virus and | 6 |
shown to increase | 6 |
gene of sars | 6 |
the positive samples | 6 |
reported that the | 6 |
acidification of the | 6 |
for orf a | 6 |
gastroenteritis in the | 6 |
with conventional rt | 6 |
alternately inverted repeats | 6 |
of these samples | 6 |
consists of the | 6 |
selected as the | 6 |
of the colorimetric | 6 |
heidelberg university hospital | 6 |
large amount of | 6 |
lamp reaction with | 6 |
a similar sensitivity | 6 |
lamp assay detected | 6 |
flp and blp | 6 |
are used to | 6 |
false negative results | 6 |
white spot syndrome | 6 |
around the world | 6 |
clinical specimens from | 6 |
buffer and stored | 6 |
they have no | 6 |
nucleotide sequence of | 6 |
are used for | 6 |
article can be | 6 |
r min for | 6 |
determined by the | 6 |
may be a | 6 |
incubated in a | 6 |
this article is | 6 |
and incubated for | 6 |
spot syndrome virus | 6 |
positive results were | 6 |
and subjected to | 6 |
effusions of cats | 6 |
detection of isothermal | 6 |
and orf ab | 6 |
time pcr in | 6 |
was tested using | 6 |
a and the | 6 |
thai et al | 6 |
laboratory confirmation of | 6 |
diagnostic tool for | 6 |
can be achieved | 6 |
stepone tm system | 6 |
small cell lung | 6 |
to be highly | 6 |
volume of l | 6 |
of samples with | 6 |
ecas for hais | 6 |
detection of zikv | 6 |
sensitivities of the | 6 |
c a a | 6 |
detection and quantitation | 6 |
molecular and serological | 6 |
this method is | 6 |
set for the | 6 |
copies of sars | 6 |
analytical sensitivity of | 6 |
detection of norovirus | 6 |
for samples with | 6 |
rna extraction for | 6 |
time polymerase chain | 6 |
have been identified | 6 |
of chik fever | 6 |
with those obtained | 6 |
and no cross | 6 |
no template control | 6 |
this case report | 6 |
to the detection | 6 |
beclin and atg | 6 |
at least one | 6 |
incubated at c | 6 |
as determined by | 6 |
risk of contamination | 6 |
sensitive than pcr | 6 |
blood mononuclear cells | 6 |
with a lateral | 6 |
amplifi cation is | 6 |
need for expensive | 6 |
optimization of the | 6 |
samples were obtained | 6 |
of hcv rna | 6 |
answer detection of | 6 |
provided by dr | 6 |
and reaction time | 6 |
did not show | 6 |
during the reaction | 6 |
cats with and | 6 |
hepatitis a virus | 6 |
a biosafety level | 6 |
followed by the | 6 |
evaluation of loop | 6 |
determine the sensitivity | 6 |
as mentioned above | 6 |
and feces by | 6 |
swab samples from | 6 |
isothermal amplification loop | 6 |
of the severe | 6 |
sars and mers | 6 |
in our previous | 6 |
identification of the | 6 |
also been shown | 6 |
two inner primers | 6 |
sent to the | 6 |
is complementary to | 6 |
the detection system | 6 |
similar to the | 6 |
during the early | 6 |
and the n | 6 |
as an alternative | 6 |
clinical evaluation of | 6 |
reverse transcription pcr | 6 |
commercial rna extraction | 6 |
primers used in | 6 |
at z c | 6 |
gene specific rt | 6 |
at a single | 6 |
to be positive | 6 |
igg antibodies in | 6 |
treated with naoh | 6 |
lamp master mix | 6 |
genome by loop | 6 |
all reactions were | 6 |
were purchased from | 6 |
we tested the | 6 |
molecular characterization of | 6 |
in this case | 6 |
of n gene | 6 |
based on its | 6 |
detection of egfr | 6 |
of cellular autophagy | 6 |
it is a | 6 |
ensure that the | 6 |
amplification method that | 6 |
and minor gii | 6 |
lamp was found | 6 |
of z c | 6 |
of the fluorescent | 6 |
added to each | 6 |
the tubes were | 6 |
to detect and | 6 |
methods to detect | 6 |
lamp reaction can | 6 |
of swab samples | 6 |
lowest detection limits | 6 |
gel stained with | 6 |
virus rapid and | 6 |
reactions were incubated | 6 |
by the institutional | 6 |
as compared to | 6 |
for the prevention | 6 |
of template rna | 6 |
number of samples | 6 |
amplification assay rapid | 6 |
added into the | 6 |
mixtures were incubated | 6 |
cap of the | 6 |
or heat block | 6 |
of the genome | 6 |
open reading frame | 6 |
from sample collection | 6 |
detection of viruses | 6 |
of this rt | 6 |
is difficult to | 6 |
of the b | 6 |
the containment of | 6 |
time pcr and | 6 |
were positive in | 6 |
and accurate diagnosis | 6 |
studies have reported | 6 |
and ml of | 6 |
u of bst | 6 |
detection with crispr | 6 |
at the poc | 6 |
detection rates of | 6 |
was selected as | 6 |
were extracted from | 6 |
use of loop | 6 |
detection system for | 6 |
to detect all | 6 |
to accelerate the | 6 |
iwamoto et al | 6 |
by measuring the | 6 |
novel coronavirus from | 6 |
both autophagy and | 6 |
g g type | 6 |
and easy detection | 6 |
with pneumonia in | 6 |
or heating block | 6 |
that were positive | 6 |
a simple water | 6 |
dna polymerase to | 6 |
zika virus infection | 6 |
ability to amplify | 6 |
consisted of the | 6 |
be performed using | 6 |
of the rapid | 6 |
human papillomavirus type | 6 |
on the detection | 6 |
of dna development | 6 |
responsible for the | 6 |
obtained from patients | 6 |
with a single | 6 |
monocytogenes dna template | 6 |
able to amplify | 6 |
lamp amplification products | 6 |
in vitro transcription | 6 |
number of clinical | 6 |
belongs to the | 6 |
is performed in | 6 |
structure of the | 6 |
results were observed | 6 |
traditional chinese medicine | 6 |
have shown that | 6 |
that they have | 6 |
nasopharyngeal swabs and | 6 |
abbott id now | 6 |
the introduction of | 6 |
n gene was | 6 |
a dilution series | 6 |
to establish a | 6 |
serum samples were | 6 |
ml reaction volume | 6 |
the pocket warmer | 6 |
used for lamp | 6 |
the method of | 6 |
assay as a | 6 |
has the potential | 6 |
a report of | 6 |
products were separated | 6 |
the sensitivity was | 6 |
we did not | 6 |
chinese academy of | 6 |
approved the final | 6 |
t and aldh | 6 |
morbidity and mortality | 6 |
amplification methods for | 6 |
in this paper | 6 |
min in the | 6 |
was lower than | 6 |
method for diagnosis | 6 |
the loop primer | 6 |
the magnesium pyrophosphate | 6 |
to the rt | 6 |
the early stages | 6 |
using an isothermal | 6 |
been successfully applied | 6 |
and the detection | 6 |
assay was evaluated | 6 |
presence of the | 6 |
of h avian | 6 |
lei et al | 6 |
of the cases | 6 |
that was developed | 6 |
s and s | 6 |
acid detection with | 6 |
explorer v software | 6 |
in a final | 6 |
bath or heat | 6 |
a very low | 6 |
in figure a | 6 |
rna mini kit | 6 |
the application of | 6 |
detection of chlamydia | 6 |
ice for min | 6 |
a sensitive and | 6 |
and validation of | 6 |
with the conventional | 6 |
green i dye | 6 |
for the reaction | 6 |
and the assay | 6 |
zheng et al | 6 |
study was supported | 6 |
presence of a | 6 |
potential of rt | 6 |
the results are | 6 |
we found that | 6 |
the robustness of | 6 |
dilution of the | 6 |
samples using the | 6 |
to perform the | 6 |
the minimum concentration | 6 |
template for rt | 6 |
were tested using | 6 |
used in a | 6 |
were collected in | 6 |