bigram

This is a table of type bigram and their frequencies. Use it to search & browse the list to learn more about your study carrel.

bigram	frequency
isothermal amplification	908
mediated isothermal	784
lamp assay	653
nucleic acid	330
reverse transcription	307
rapid detection	261
lamp reaction	213
transcription loop	158
clinical samples	154
dna polymerase	140
rna extraction	137
chain reaction	134
viral rna	134
time pcr	130
respiratory syndrome	125
lamp method	118
lamp assays	117
amplification method	114
agarose gel	111
polymerase chain	110
nucleic acids	105
amplification assay	103
acute respiratory	102
acid amplification	97
detection limit	94
gel electrophoresis	92
severe acute	91
granted medrxiv	88
author funder	88
dna amplification	87
lamp primers	87
copyright holder	86
sybr green	85
primer sets	85
reverse transcriptase	84
loop primers	81
primer set	79
time rt	79
version posted	77
novel coronavirus	76
conventional rt	76
syndrome coronavirus	76
lamp products	75
lamp reactions	74
amplifi cation	73
reaction mixture	70
pcr assay	69
positive samples	68
real time	68
peer review	66
strand displacement	65
virus infection	65
doc id	63
cord uid	63
infectious diseases	63
visual detection	61
green i	61
time reverse	60
amplification reaction	60
high sensitivity	60
positive results	60
dna extraction	59
rapid diagnosis	57
zika virus	56
loop mediated	56
loopmediated isothermal	56
viral load	56
lateral flow	55
medrxiv preprint	55
japanese encephalitis	53
qpcr assay	53
magnetic bead	53
target dna	53
clinical specimens	51
isothermal conditions	50
based amplification	50
negative samples	48
influenza virus	48
detection method	48
dengue virus	47
rna polymerase	47
magnesium pyrophosphate	47
reaction time	46
based detection	46
water bath	45
swab samples	45
color change	45
mycobacterium tuberculosis	45
per reaction	45
using loop	45
virus isolation	44
ct values	44
blood samples	43
conventional pcr	43
new england	42
performed using	42
whole blood	42
orf ab	41
lamp primer	41
copies per	41
escherichia coli	41
master mix	41
detection methods	41
inner primer	41
west nile	40
england biolabs	40
negative control	40
bst dna	40
cell death	40
dna synthesis	39
target sequence	39
sample preparation	39
disease virus	39
nasopharyngeal swabs	39
serial dilutions	38
copy number	38
specific primers	38
acid sequence	37
public health	37
amplification using	36
free water	36
high specificity	36
molecular detection	36
naked eye	36
qlamp assay	36
copies reaction	36
acid testing	36
specific amplification	35
encephalitis virus	34
porcine circovirus	34
reaction temperature	34
highly specific	34
outer primers	34
pcr assays	34
lamp amplification	34
ethidium bromide	33
positive control	33
rna viruses	33
amplification products	32
time detection	32
present study	32
total rna	32
step rt	32
coronavirus disease	32
serum samples	31
inner primers	31
sensitive detection	31
patient samples	31
false positives	31
swab specimens	31
porcine parvovirus	31
sputum samples	31
clinical diagnosis	31
made available	30
rights reserved	30
cancer cells	30
higher sensitivity	30
lamp detection	30
highly sensitive	30
je virus	30
reaction volume	30
nile virus	30
reaction mixtures	30
reuse allowed	29
amplified products	29
standard curve	29
eiken chemical	29
direct detection	29
constant temperature	29
international license	29
room temperature	29
time monitoring	29
rna isolation	29
nested rt	29
primer design	29
without permission	29
allowed without	29
primers targeting	29
chik virus	28
polymerase amplification	28
amplification efficiency	28
applied biosystems	28
avian influenza	28
genomic dna	28
dried blood	28
bead rna	28
primer mix	28
rna samples	28
fold serial	28
detection system	28
colorimetric lamp	28
viral nucleic	28
recombinase polymerase	28
negative controls	28
six primers	28
using rt	27
posted november	27
dna template	27
syndrome virus	27
results showed	27
colorimetric rt	27
false positive	27
detection using	27
isothermal nucleic	27
acid detection	26
stranded dna	26
virus detection	26
widely used	26
commercially available	26
mycobacterium ulcerans	26
infectious peritonitis	26
molecular diagnostics	26
feline infectious	26
magnetic beads	25
specific lamp	25
target rna	25
samples collected	25
copy numbers	25
microfluidic disc	25
diagnostic test	25
rna template	25
diagnostic methods	25
pcr methods	25
newly developed	25
assay using	25
specific detection	25
novel loop	24
molecular diagnostic	24
optimal reaction	24
specific rt	24
pathogen detection	24
buruli ulcer	24
respiratory viruses	24
extraction kit	24
middle east	24
loop primer	24
amplification techniques	24
snp detection	24
detecting sars	24
isothermal amplifi	23
lung cancer	23
east respiratory	23
detection results	23
developing countries	23
united states	23
accelerated reaction	23
outer primer	23
gold standard	23
diagnostic method	23
fluorescence signal	23
rna detection	23
reaction tube	23
influenza viruses	23
detection rate	23
human papillomavirus	23
circovirus type	23
herpes simplex	23
microfluidic system	22
pcr amplification	22
pcr products	22
armored rna	22
simplex virus	22
primer explorer	22
sample types	22
newcastle disease	22
colorimetric detection	22
lamp system	22
fever virus	22
four primers	22
chikungunya virus	22
linked immunosorbent	22
conventional lamp	22
urine samples	21
well plate	21
posted july	21
rna particles	21
virus serotypes	21
lysis buffer	21
nasopharyngeal swab	21
two outer	21
qpcr assays	21
nested pcr	21
using clinical	21
feline coronavirus	21
chemical co	21
viral genome	21
lamp test	21
virus rna	20
copies ml	20
african genotype	20
lamp results	20
induced autophagy	20
egfr mutations	20
amplification technique	20
generation sequencing	20
within min	20
blood spots	20
gene amplification	20
virus type	20
displacement activity	20
dilution series	20
reaction mix	20
detect sars	20
diagnostic laboratory	20
primers used	20
mediated autophagy	20
viral infections	20
pcr method	19
gene sequences	19
gargle lavage	19
intravenous alteplase	19
described previously	19
pcr kit	19
authors declare	19
infected shrimp	19
igg antibodies	19
infected patients	19
peripheral blood	19
purified dna	19
assay developed	19
rna virus	19
listeria monocytogenes	19
displacement amplification	19
iu ml	19
fluorescent dye	19
dna products	19
rna extracted	19
fluorescence detection	18
copies tube	18
time fluorescence	18
turbidity derived	18
genbank accession	18
posted may	18
clinical diagnostic	18
lamp product	18
carotid artery	18
mm tris	18
hybridization chain	18
amplification assays	18
accession number	18
immunosorbent assay	18
ct value	18
pyrophosphate formation	18
extracted rna	18
template dna	18
positive result	18
supplementary table	18
positive reaction	18
using reverse	18
isothermal dna	18
thermal cycler	18
pocket warmer	18
amplified dna	18
hbv dna	18
lamp amplicons	18
false negatives	17
hpv type	17
developed rt	17
amplification rapid	17
rolling circle	17
induces autophagy	17
target gene	17
filter paper	17
limited settings	17
diagnostic tool	17
distinct regions	17
amplification methods	17
samples using	17
visual inspection	17
viral dna	17
direct naats	17
detection sensitivity	17
virus rapid	17
denv infection	17
uv light	17
base apparatus	17
backward inner	17
displacement dna	17
results indicated	17
host cell	17
clinical signs	17
forward inner	17
infectious bronchitis	17
pcr system	17
transmissible gastroenteritis	17
isolated rna	17
acute phase	17
less sensitive	17
molecular diagnosis	17
using lamp	17
thermal cycling	17
rna copies	16
sry gene	16
amplification detection	16
circle amplification	16
opiph system	16
without rna	16
detect viral	16
viral replication	16
assay showed	16
one step	16
detection limits	16
specific dna	16
vhs virus	16
target sequences	16
hpv dna	16
fecal samples	16
reaction tubes	16
situ rt	16
wasting syndrome	16
coronavirus sars	16
disease control	16
alteplase infusion	16
world health	16
rna using	16
syncytial virus	16
using primer	16
two loop	16
respiratory syncytial	16
tested positive	16
designed using	16
based assays	16
obtained using	16
laboratory diagnosis	16
stranded rna	16
expensive equipment	16
health organization	16
early diagnosis	16
lamp reagents	16
quantitative pcr	15
serially diluted	15
qpcr using	15
cancer cell	15
thermo fisher	15
hcv rna	15
total volume	15
positive reactions	15
simple visual	15
tested using	15
induce autophagy	15
crispr cas	15
multisystemic wasting	15
commonly used	15
dna sequences	15
diagnostic accuracy	15
hbv lamp	15
acid extraction	15
bronchitis virus	15
immunodeficiency virus	15
visual rt	15
immune system	15
isothermal device	15
cell culture	15
dependent amplification	15
phenol red	15
multiplex pcr	15
lamp protocol	15
ab gene	15
turkey coronavirus	15
ischemic stroke	15
tuberculosis complex	15
clinical sample	15
negative results	15
serotype specific	15
novel rt	15
centrifugal microfluidic	15
great potential	15
iso buffer	15
dna fragments	15
predictive value	15
host cells	15
direct pcr	15
human immunodeficiency	15
analytical sensitivity	14
rapid diagnostic	14
cost effective	14
distilled water	14
large numbers	14
conserved region	14
rapid molecular	14
suspected covid	14
binding proteins	14
upper respiratory	14
amplification technologies	14
informed consent	14
pharyngeal swab	14
rapid identification	14
dna purification	14
quantitative rt	14
diagnostic testing	14
viral pathogens	14
restriction enzyme	14
rainbow trout	14
fisher scientific	14
reaction buffer	14
assay detection	14
ebola virus	14
hydroxynaphthol blue	14
clinical trials	14
based methods	14
pcr detection	14
postweaning multisystemic	14
extraction protocol	14
optical detection	14
viral hemorrhagic	14
previous studies	14
coronavirus using	14
dna extracts	14
positive controls	14
integrated isothermal	14
viral infection	14
results obtained	14
autophagosome formation	14
amplification test	14
even though	14
causative agent	14
infectious disease	13
dna detection	13
nucleotide sequence	13
swab sample	13
heating block	13
mm dntps	13
final volume	13
infected individuals	13
detection assays	13
pseudorabies virus	13
confidence intervals	13
dengue viruses	13
body cavity	13
deficient mice	13
saliva samples	13
porcine reproductive	13
neurodegenerative diseases	13
primer dimers	13
elution buffer	13
transcriptase polymerase	13
reaction products	13
time loop	13
flow dipstick	13
dna loop	13
detecting ibv	13
mini kit	13
prv ge	13
yellow head	13
amplification kit	13
selective autophagy	13
viral loads	13
diagnostic assays	13
also tested	13
detection kits	13
ns antigen	13
reaction conditions	13
commercial rt	13
pcr test	13
care diagnostics	13
conducted using	13
heat block	13
like pattern	13
rna purification	13
extraction methods	13
sars coronavirus	13
extracted using	13
fl uorescent	13
using real	12
pharyngeal swabs	12
tissue samples	12
oxidative stress	12
autophagy genes	12
previous study	12
conventional one	12
temperature control	12
detection mix	12
mumps virus	12
ns serotype	12
genome sequences	12
mediated amplification	12
assays targeting	12
foster city	12
protease inhibitors	12
transcription polymerase	12
internal control	12
sequence analysis	12
hemorrhagic septicaemia	12
dependent rna	12
pcr results	12
sample collection	12
positive signal	12
time quantitative	12
polymerase activity	12
vhs rna	12
digital pcr	12
head virus	12
samples tested	12
exponential amplification	12
time points	12
fimbriae gene	12
negative predictive	12
molecular methods	12
tcid ml	12
six distinct	12
molecular assays	12
virus replication	12
designed primers	12
ethics committee	12
clinical symptoms	12
detection systems	12
test results	12
ns ag	12
cycle threshold	12
time required	12
qpcr detection	12
based tests	12
amplification curves	12
critically ill	12
poc diagnostics	12
immune response	12
swine fever	12
gene sequence	12
assay may	12
necrosis virus	12
nasal swabs	12
detection time	12
reaction using	12
blood spot	12
previously described	11
lowest detection	11
sex determination	11
method rapid	11
clinical application	11
care testing	11
clinical use	11
results indicate	11
gene detection	11
agarose gels	11
type prv	11
assay development	11
amplification process	11
diagnostic tests	11
rna molecules	11
chamber iii	11
diarrhea virus	11
single reaction	11
fold higher	11
primers loop	11
type strains	11
digital lamp	11
sensitivity test	11
highly conserved	11
single step	11
salmonella enterica	11
simultaneous detection	11
oligonucleotide primers	11
two primers	11
mouth disease	11
visualized using	11
lymph nodes	11
closely related	11
rna standard	11
single strand	11
extraction method	11
dna rna	11
chlamydia trachomatis	11
also known	11
touchdown lamp	11
serological tests	11
samples obtained	11
two assays	11
mm mgso	11
turnaround time	11
chik fever	11
virus genome	11
false negative	11
lamp technology	11
gastroenteritis coronavirus	11
disposable micro	11
clinical validation	11
pcr reaction	11
total reaction	11
amplified product	11
assays using	11
glu lys	11
amplification time	11
transport media	11
two different	11
serial dilution	11
respiratory tract	11
competing interests	11
uvrd helicase	11
new generation	11
isothermal mastermix	11
primer sequences	11
human herpesvirus	11
direct sequencing	11
standard curves	11
posted august	11
amplification tests	11
resource settings	11
cell lung	11
single tube	11
porcine epidemic	11
egfr mutation	11
distinct sequences	11
table ii	10
human coronaviruses	10
using primers	10
bip primers	10
specially designed	10
two inner	10
low viral	10
complementary sequence	10
uv transilluminator	10
cohort i	10
covert mortality	10
test kit	10
pcr product	10
dna rapid	10
factor receptor	10
epidemic diarrhea	10
inhibiting autophagy	10
intercalating dye	10
university hospital	10
human genomic	10
isothermal master	10
autophagic vacuoles	10
virol methods	10
viral diseases	10
epidermal growth	10
copper plates	10
spike protein	10
lavage samples	10
swine transmissible	10
specific reaction	10
reliable detection	10
clinical management	10
gene rt	10
qiaamp viral	10
quantitative detection	10
novel sars	10
study showed	10
methods require	10
gene primers	10
dna sequencing	10
time consuming	10
lamp may	10
reaction system	10
virus dna	10
rna amplification	10
detection rates	10
healthy individuals	10
assay detected	10
amplification reactions	10
molecular techniques	10
human coronavirus	10
hcr reaction	10
ulcerans infection	10
infectious agents	10
nervous system	10
template rna	10
wide range	10
simple method	10
lamp technique	10
pcr using	10
classical swine	10
enterotoxigenic escherichia	10
colour change	10
positive predictive	10
quantitative real	10
two samples	10
blue dye	10
molecular test	10
positive rt	10
wild type	10
ec nv	10
akt mtor	10
buccal swab	10
rna per	10
two sets	10
independent cohort	10
dna strand	10
danon disease	10
structural proteins	10
aldh glu	10
two methods	10
different concentrations	10
forward primer	10
asian genotype	10
novel reverse	10
using rna	10
amplification development	10
growth factor	10
inhibit autophagy	10
pulmonary adenocarcinoma	10
human respiratory	10
molecular weight	10
autophagy autophagy	10
fip bip	10
tris buffer	10
likelihood ratios	10
methods doi	10
septicaemia virus	10
samples containing	10
optigene ltd	10
early stages	9
open access	9
warmstart colorimetric	9
multiplex rt	9
amplification product	9
minor gii	9
epithelial cells	9
field conditions	9
recognize six	9
zoster virus	9
pcr tests	9
different primer	9
care test	9
realtime pcr	9
optimal temperature	9
chronic hepatitis	9
sample type	9
single nucleotide	9
without dna	9
ph indicator	9
prevalent gii	9
commercial kit	9
purified using	9
high risk	9
patients infected	9
untranslated region	9
stem cell	9
coronavirus infection	9
rdrp gene	9
extraction kits	9
direct amplification	9
small molecule	9
scale testing	9
chik infection	9
class ii	9
template control	9
chest ct	9
microfluidic chip	9
rapid test	9
detection assay	9
reaction solution	9
staphylococcus aureus	9
flow assay	9
iron homeostasis	9
qpcr test	9
respiratory samples	9
tumor progression	9
high throughput	9
effective diagnostic	9
pcv lamp	9
time turbidimeter	9
lower sensitivity	9
care home	9
molecular tests	9
using two	9
eight distinct	9
alere i	9
confirmed cases	9
life technologies	9
antiviral drugs	9
ns gene	9
amplification date	9
quantifi cation	9
target nucleic	9
clinical evaluation	9
purified rna	9
target amplification	9
open reading	9
well plates	9
situ hybridization	9
based assay	9
bst polymerase	9
pot real	9
confidence interval	9
potential use	9
pcr machine	9
oropharyngeal swab	9
viral antigens	9
extraction step	9
using isothermal	9
cdc real	9
case report	9
intercalating dyes	9
stroke treatment	9
measured using	9
polymerase promoter	9
infected samples	9
primary material	9
rna fragment	9
novel nucleic	9
overall sensitivity	9
emerging viruses	9
qpcr results	9
trained personnel	9
nucleotide polymorphisms	9
human influenza	9
detection unit	9
respiratory specimens	9
er stress	9
mm edta	9
mm mgcl	9
artery stenting	9
powdery mildew	9
enzyme mix	9
recent advances	9
asymptomatic carrier	9
times higher	9
amplification combined	9
expensive instrumentation	9
polymerase gene	9
gmo detection	9
melt curve	9
control group	9
cmnv rt	9
specifically designed	9
recent years	9
conventional kit	9
creative commons	9
new coronavirus	9
serum specimens	9
dna development	9
viral sequences	9
loop dna	9
allantoic fluid	9
cavity effusions	9
global pandemic	9
autophagy may	9
sequence based	8
white precipitate	8
protein gene	8
using colorimetric	8
nucleocapsid protein	8
gene specific	8
innate immune	8
ha gene	8
lamp showed	8
field detection	8
amv reverse	8
converting enzyme	8
lamp methods	8
genefinder tm	8
may also	8
loop structure	8
conserved regions	8
pcr inhibitors	8
processing time	8
recent outbreak	8
acute ischemic	8
experimentally infected	8
cellular autophagy	8
amplification loop	8
ml reaction	8
cell survival	8
inverted repeats	8
internal primers	8
testing methods	8
nonspecific amplification	8
light source	8
including two	8
target genes	8
supplementary material	8
using ph	8
detection platform	8
method using	8
optimized rt	8
stem loop	8
tissue kit	8
positive rate	8
lamp pcr	8
reference laboratory	8
study design	8
cation method	8
acute gastroenteritis	8
fully automated	8
zikv infection	8
supporting information	8
amplification system	8
cohort study	8
fi gure	8
traditional chinese	8
time assays	8
cell surface	8
without loop	8
primer combinations	8
based diagnostics	8
endoplasmic reticulum	8
transcription loopmediated	8
correlation coefficient	8
methods used	8
dna strands	8
grape powdery	8
kidney disease	8
hais due	8
reaction units	8
assay results	8
early stage	8
primer specificity	8
several studies	8
erysiphe necator	8
reaction unit	8
sets targeting	8
infl uenza	8
based testing	8
reverse primer	8
beclin expression	8
forward loop	8
aichi virus	8
signal amplification	8
stem cells	8
routine rt	8
sequence alignment	8
based real	8
data analysis	8
infected cells	8
mean detection	8
signaling pathways	8
primers fip	8
hda system	8
denv rt	8
lamp testing	8
test kits	8
atg expression	8
large fragment	8
viral transport	8
amplification technology	8
clinical features	8
developed using	8
time nucleic	8
dependent isothermal	8
cell line	8
turbidity detection	8
detection techniques	8
immune responses	8
reaction chamber	8
initial denaturation	8
suspected patients	8
rna sequences	8
disease caused	8
microfluidic devices	8
easy detection	8
dna polymerases	8
amplified using	8
coronavirus rna	8
simple detection	8
cohort ii	8
i influenza	8
step reverse	8
pregnant women	8
optimized lamp	8
direct nucleic	8
first report	8
cell lines	8
designed based	8
hemagglutinin gene	8
evaluated using	8
single stranded	8
ndv strains	8
fip primers	8
agarose beads	8
mouse model	8
pcr based	8
smartphone camera	8
roundup ready	8
nv detection	8
specific real	8
global health	8
time point	7
fluorescence intensity	7
mineral oil	7
multiplexed rt	7
target snp	7
genomic rna	7
two additional	7
time lamp	7
pcr cycler	7
protein aggregates	7
institutional review	7
automated microfluidic	7
set targeting	7
positive amplification	7
san diego	7
valproic acid	7
minimum concentration	7
genomic sequences	7
severe cases	7
pcr reactions	7
plasmon resonance	7
id now	7
pot visual	7
autophagy research	7
nv gi	7
sample rods	7
science foundation	7
qpcr kit	7
genome amplification	7
diagnostic sensitivity	7
assay targeting	7
diagnosis using	7
accurate diagnostic	7
spore samplers	7
rapid rt	7
previously reported	7
base pairs	7
lamp master	7
duck hepatitis	7
plasmid standards	7
enzymatic amplification	7
specific primer	7
excessive autophagy	7
transport medium	7
two major	7
accelerating primer	7
blood sample	7
mhc class	7
binding protein	7
diagnostic tools	7
template input	7
serological assays	7
plasmid dna	7
serial diluted	7
stepone tm	7
greater sensitivity	7
final extension	7
reverse primers	7
bronchoalveolar lavage	7
based rna	7
extremely high	7
emerging infectious	7
isothermal condition	7
microfluidic device	7
taq polymerase	7
human cells	7
white spot	7
molecular beacons	7
penaeus monodon	7
recently developed	7
detection based	7
lamp amplifi	7
autophagy inhibition	7
sample size	7
fold dilutions	7
lamp result	7
standard rt	7
four sets	7
final concentration	7
north america	7
related viruses	7
high viral	7
rna target	7
polymerase used	7
online version	7
transcriptase loop	7
dna helicase	7
rna fragments	7
samples isolated	7
herpesvirus dna	7
containing ml	7
asymptomatic carriers	7
centrifugal platform	7
autophagy induction	7
denv serotypes	7
mortality disease	7
molecular beacon	7
detection kit	7
virus loop	7
mixture contained	7
negative reactions	7
poc test	7
respiratory disease	7
neisseria gonorrhoeae	7
routine clinical	7
prevent cross	7
specialized equipment	7
based lamp	7
diagnostic platform	7
qiacube extraction	7
sense sequence	7
cost effectiveness	7
test using	7
lamp mixture	7
clinical setting	7
established rt	7
gene fragment	7
camostat mesylate	7
positive clinical	7
analyzed using	7
automated centrifugal	7
subsequent amplification	7
ibv strains	7
clostridium difficile	7
diagnostic potential	7
min using	7
denv ns	7
successfully applied	7
zikv strains	7
lamp target	7
monocytogenes dna	7
coat protein	7
hemorrhagic fever	7
temperature range	7
autophagy protein	7
transcription pcr	7
virus strains	7
require expensive	7
primer regions	7
capsid protein	7
membrane protein	7
biological substances	7
specifi city	7
bacterial infection	7
coronavirus rapid	7
chamber i	7
dna templates	7
reaction denv	7
innate immunity	7
pcr analysis	7
low cost	7
review board	7
specific loop	7
small sample	7
spore quantities	7
high numbers	7
electron microscopy	7
growing season	7
defective autophagy	7
white shrimp	7
ncov infection	7
gene copy	7
innovative gene	7
examined using	7
high degree	7
autoimmune diseases	7
viral particles	7
cell lysis	7
true positive	7
specimens collected	7
direct rt	7
integrated microfluidic	7
hepatocellular carcinoma	7
like structures	7
negative diagnosis	7
fluorescent detection	7
taqman real	7
simple operation	7
nasopharyngeal specimens	7
hot swab	7
accurate diagnosis	7
two cohorts	7
poc applications	7
primer pairs	7
mutation detection	7
heidelberg university	7
low concentrations	7
patient sample	7
diagnostic qpcr	7
antibody responses	7
also used	7
detect rna	7
ns rt	7
novel method	7
extraction using	7
stool samples	7
virus isolates	7
care settings	7
reactions using	7
lamp using	7
dna extracted	7
signaling pathway	7
analysis using	7
direct testing	7
acute stage	7
gargle lavages	7
confirmed covid	7
lamp mix	7
negative rt	7
following intravenous	7
double stranded	7
matrix gene	7
assay rapid	7
oilseed rape	7
scored negative	7
surface plasmon	7
vp gene	7
showed high	7
reagent injection	7
two pairs	7
snps detection	7
sensitive dyes	7
written informed	7
time nasba	7
commercial kits	7
amino acid	7
ccc pipeline	7
detected using	7
proliferative kidney	7
dna accelerated	7
signal enhancement	6
complementary strand	6
free molecular	6
rapid method	6
pipette tips	6
chinese medicine	6
control line	6
one single	6
genetic variation	6
nm annealed	6
personalized medicine	6
clinical study	6
molecular characterization	6
large amount	6
directly added	6
respiratory viral	6
per sample	6
avian myeloblastosis	6
crispr diagnostics	6
patient management	6
screening method	6
calculated using	6
simulation samples	6
ge gene	6
fecal specimens	6
negative patients	6
rapid reaction	6
products using	6
least one	6
turbidity assay	6
like bands	6
inhibits autophagy	6
transcription step	6
mm thick	6
one hour	6
synthetic sars	6
denv rna	6
software program	6
speed centrifugation	6
target rt	6
alternately inverted	6
amplify dna	6
plasmodium falciparum	6
abbott id	6
broad range	6
per ml	6
based signal	6
spot syndrome	6
mm kcl	6
unfolded protein	6
different primers	6
using fluorescent	6
pacific white	6
porcine circoviruses	6
antigen presentation	6
amplification times	6
different temperatures	6
membrane fusion	6
high consistency	6
background dna	6
rna sequence	6
primers specific	6
serological methods	6
protein response	6
lamp amplified	6
primer designing	6
molecular mechanisms	6
dengue infection	6
one study	6
life sciences	6
takes place	6
showed higher	6
tm system	6
water samples	6
isothermal detection	6
new method	6
amplification speed	6
poor settings	6
ndv alone	6
amplification step	6
cdna synthesis	6
independent sequences	6
oncorhynchus mykiss	6
autophagy via	6
pvx rna	6
time polymerase	6
rapid screening	6
autophagy inhibitors	6
regulate autophagy	6
zikv sequences	6
diagnostic performance	6
quantitative results	6
tested negative	6
papillomavirus type	6
six different	6
determined using	6
detected within	6
fluorescence signals	6
sensitivity compared	6
table iv	6
infected pigs	6
cell growth	6
multiple displacement	6
lamp devices	6
important role	6
backward outer	6
commercial rna	6
natural science	6
respiratory pathogens	6
blood plasma	6
lamp approach	6
pyrophosphate ions	6
valuable tool	6
internal carotid	6
using crispr	6
medical sciences	6
loop dnas	6
dna samples	6
based method	6
since lamp	6
rna kit	6
similar sensitivity	6
avian infectious	6
autophagic degradation	6
also performed	6
food samples	6
biosafety level	6
generated using	6
human blood	6
randomly selected	6
purification step	6
northwest university	6
ndv detection	6
respiratory infections	6
coronavirus pneumonia	6
forming units	6
homozygous mutation	6
promoter sequence	6
dao thi	6
accession numbers	6
extremely useful	6
gel stained	6
clinical trial	6
diabetes mellitus	6
single rt	6
cervical cancer	6
slit lamp	6
commercial isothermal	6
bowenoid papulosis	6
standard method	6
reading frame	6
mycoplasma pneumoniae	6
denv serotype	6
min time	6
protein kinase	6
standard operating	6
provide appropriate	6
ncov detection	6
complementary dna	6
pandemic influenza	6
culture medium	6
critical review	6
sample handling	6
traditional pcr	6
dna sequence	6
necator dna	6
small cell	6
breast cancer	6
viruses including	6
amplification results	6
novel anti	6
autophagic cell	6
tumor cell	6
dnase rnase	6
specificity study	6
increase sensitivity	6
assay demonstrated	6
successfully detected	6
multiple loops	6
health emergency	6
viruses using	6
detecting pedv	6
first time	6
west sussex	6
endemic countries	6
two hospitals	6
different genotypes	6
molecular biology	6
like viruses	6
lamp shield	6
without fip	6
mortality rate	6
ivt rna	6
digital droplet	6
ex taq	6
also showed	6
fold dilution	6
uorescent dye	6
table i	6
probe sets	6
immune cells	6
answer detection	6
displacement reaction	6
kinase complex	6
vitro transcription	6
complete genome	6
south america	6
customized instrument	6
pcr positive	6
volume containing	6
care diagnostic	6
melting temperature	6
myeloblastosis virus	6
method may	6
commons attribution	6
infected fish	6
phosphate buffered	6
similar results	6
fine needle	6
blot hybridization	6
nonstructural proteins	6
microbial infections	6
mononuclear cells	6
rna genome	6
infected bhk	6
fluorescence microscopy	6
laboratory confirmation	6
four samples	6
mtor pathway	6
ml eppendorf	6
sensitive loop	6
regulates autophagy	6
southeast asia	6
likelihood ratio	6
early detection	6
rna mini	6
field applications	6
animal health	6
lamp detected	6
gene expression	6
helicase dependent	6
simple water	6
i dye	6
also developed	6
measles virus	6
cerebrospinal fluid	6
genie ii	6
heat treatment	6
targeting orf	6
ndv rna	6
dna extractions	6
clinical laboratories	6
genotype sequences	6
respiratory secretions	6
melting curve	6
mediated detection	6
chinese academy	6
standard deviation	6
review editing	6
loop structures	6
gii primer	6
assay system	6
loops formed	6
cancer therapy	6
genital lesions	6
ap pathway	6
previous reports	6
plasma samples	6
dna polymerization	6
dna directly	6
using molecular	6
target region	6
patients suspected	6
virus development	6
using dna	6
inhibitory effect	6
sangon biotech	6
isothermal techniques	6
based dlamp	6
inoculum detection	6
asymptomatic covid	6
effective method	6
isothermal amplificatio	6
eppendorf tube	6
magnetic rack	6
starting structure	6
metal block	6
blood mononuclear	6
yellow fever	6
strains used	6
guanidine hydrochloride	6
tertiary care	6
integrated dna	6
lavage fluid	6
clinical practice	6
poc testing	6
temperature cycling	5
dis doi	5
amplify target	5
gp helicase	5
coronavirus loop	5
three primer	5
bovine serum	5
may improve	5
crude dna	5
simple isothermal	5
significant difference	5
ngs confirmation	5
overall specificity	5
lupus erythematosus	5
quantified using	5
incubation time	5
green color	5
quantitative assay	5
operating procedures	5
virus infections	5
related protein	5
supplementary data	5
multiplex loop	5
products showed	5
control template	5
valley fever	5
chikungunya fever	5
close contact	5
way junction	5
major advantage	5
targeting severe	5
molecular size	5
health care	5
time turbidimetry	5
high ct	5
rapid amplification	5
biotech co	5
displacing polymerase	5
min incubation	5
portable device	5
tumor suppression	5
pcr testing	5
accessory proteins	5
selected based	5
via autophagy	5
field use	5
promising approach	5
phase serum	5
tube rt	5
dna ladder	5
heart failure	5
amplify specific	5
drug administration	5
seed plate	5
clinical serum	5
inconsistent results	5
small amount	5
assay used	5
newly designed	5
nested polymerase	5
rapid early	5
commercial qiacube	5
receptor binding	5
assays performed	5
complex samples	5
strand displacing	5
centrifugal force	5
using serial	5
true negative	5
autophagy proteins	5
detection process	5
specifically amplify	5
acid purification	5
dna replication	5
hae iii	5
gastroenteritis virus	5
situ reverse	5
plasmids containing	5
sputum specimens	5
based test	5
one primer	5
clinical manifestations	5
ms rf	5
hbv viral	5
central nervous	5
target concentrations	5
genotype zikv	5
rnase free	5
autophagy plays	5
flow icts	5
personal protective	5
dna intermediate	5
leafroll virus	5
monoclonal antibodies	5
amino acids	5
green fluorescence	5
confirmed using	5
national institute	5
reported previously	5
higher amplification	5
routine diagnostic	5
molecular grade	5
sequence replication	5
tubes containing	5
sequence complementary	5
care homes	5
protective equipment	5
low positive	5
widely available	5
dna real	5
positive patients	5
acs synthetic	5
transcriptase step	5
rotation rate	5
detection tool	5
multiplex tandem	5
supplementary information	5
monitoring methods	5
designed targeting	5
autophagy regulation	5
gg gene	5
tgev detection	5
dry swabs	5
chamber ii	5
observational cohort	5
relatively low	5
qpcr testing	5
protease inhibitor	5
tumor suppressor	5
us government	5
detection reagent	5
lamp process	5
diagnostics using	5
vitro antiviral	5
surplus material	5
field samples	5
lamp also	5
lysosomal dysfunction	5
stem lengths	5
uv illumination	5
samples also	5
quantifying template	5
visual observation	5
ill patients	5
infectious hematopoietic	5
determine whether	5
coli uvrd	5
turbidity measurement	5
org synthbio	5
nan doi	5
deletion mutation	5
virol doi	5
mosaic virus	5
throat swabs	5
increased sensitivity	5
reference strains	5
recently reported	5
fluorescent signal	5
infected culture	5
cell cycle	5
gene primer	5
transcription recombinase	5
alternative method	5
diagnostic laboratories	5
warmstart dna	5
signal magnitude	5
displacing dna	5
molecular testing	5
based rt	5
lamp procedure	5
autophagosome maturation	5
nondenatured template	5
mbs bio	5
samples rapid	5
performed without	5
specific regions	5
access article	5
listeria species	5
optimized conditions	5
synthetic rna	5
grand island	5
low concentration	5
assimilating probe	5
care nucleic	5
nbnm beads	5
systematic review	5
major role	5
step amplification	5
point mutations	5
human fecal	5
pyrophosphate precipitate	5
sample pre	5
hmg box	5
disease detection	5
hs bp	5
field application	5
reaction product	5
biotechnology co	5
laboratory testing	5
potato virus	5
based dna	5
autocycling strand	5
typically used	5
enzyme linked	5
reaction efficiency	5
likely due	5
orange color	5
disease severity	5
chemical reaction	5
cells autophagy	5
reference rt	5
transcription lamp	5
amplification real	5
available online	5
autophagy machinery	5
common respiratory	5
global public	5
standard procedures	5
strains isolated	5
detection strategy	5
gene protein	5
utm pbs	5
electrochemical detection	5
result diagnosis	5
southern india	5
ulcerans disease	5
mixture containing	5
diagnostic technique	5
two separate	5
rna directly	5
assays require	5
porcine viruses	5
strand exchange	5
min followed	5
input volume	5
test line	5
nucleotide database	5
direct swab	5
article distributed	5
identifi cation	5
condyloma acuminatum	5
sense rna	5
related genes	5
human genome	5
detected vhs	5
multiplex real	5
forward outer	5
mammalian autophagy	5
even higher	5
rift valley	5
genome sequence	5
positive lamp	5
antiplatelet medication	5
cation methods	5
transcriptional loop	5
acute denv	5
bacterial pathogens	5
potato viruses	5
naoh solution	5
molecular method	5
potato leafroll	5
primer fip	5
vhs serotypes	5
control reactions	5
also observed	5
future perspectives	5
systemic lupus	5
temple johnson	5
directly without	5
designed primer	5
hcv infection	5
direct visualization	5
fold greater	5
one microliter	5
genotyping results	5
gene based	5
aichi viruses	5
six regions	5
ic ct	5
positive test	5
reverse transcriptional	5
negative regulator	5
disease progression	5
used method	5
results demonstrated	5
gi primer	5
hematopoietic necrosis	5
zikv strain	5
international concern	5
useful tool	5
primers within	5
inconsistent samples	5
conditions using	5
correctly identified	5
lamp kit	5
clinical utility	5
may provide	5
sherlock assays	5
qiacube rna	5
explorer version	5
imaging system	5
loopamp real	5
potential therapeutic	5
ct imaging	5
taqman probes	5
differential diagnosis	5
mathematical model	5
african trypanosomes	5
care detection	5
computed tomography	5
polymerase inhibitors	5
colon cancer	5
leaf curl	5
high correlation	5
detect ibv	5
method used	5
control measures	5
swabs without	5
different types	5
dye sybr	5
high efficiency	5
quantitative analysis	5
therapeutic target	5
ppv vp	5
see table	5
selected temperature	5
shellfish diseases	5
positive cases	5
spurious amplification	5
reaction condition	5
cardiovascular diseases	5
positive using	5
may cause	5
porcine pegivirus	5
detect human	5
research institute	5
transcriptase pcr	5
amplification directly	5
new approach	5
viruses detection	5
sample nucleic	5
clinical specimen	5
detect ppv	5
ml total	5
promote cell	5
later stages	5
dna technologies	5
tract infections	5
induced apoptosis	5
acid release	5
samples positive	5
monoclonal antibody	5
noise ratio	5
amies medium	5
rna concentration	5
results show	5
better sensitivity	5
novel dna	5
higher sensitivities	5
negative likelihood	5
potential cross	5
samples diluted	5
coronavirus severe	5
isolation kit	5
diluted sybr	5
biology pubs	5
kindly provided	5
genbank database	5
data curation	5
order nidovirales	5
egfr pcr	5
mesenchymal stem	5
small amounts	5
coronavirus associated	5
sample matrix	5
scientific research	5
gradient pcr	5
reference genome	5
large number	5
cold metal	5
heat inactivation	5
four different	5
primer pair	5
one sample	5
rna technology	5
qlamp detection	5
exact test	5
autophagosome biogenesis	5
ph value	5
ml lamp	5
rpa reaction	5
rna standards	5
pcr primers	5
hcr products	5
tissue culture	5
dna using	5
detecting viral	5
needle aspirates	5
human monoclonal	5
nicking enzyme	5
channel layer	5
positive sample	5
per well	5
financial support	5
buffered saline	5
additional primers	5
threshold time	5
step process	5
specificity test	5
etiological agent	5
future studies	5
annealing temperature	5
significant number	5
approximately min	5
fatal disease	5
associated protein	5
uv lamp	5
community settings	5
visualized via	5
rna transcripts	5
ndv templates	5
specific reactions	5
spin column	5
comparable sensitivity	5
therascreen egfr	5
sensitive method	5
high mortality	5
funding acquisition	5
highly desirable	5
equipped laboratories	5
arbovirus infection	5
also applied	5
ulk complex	5
droplet pcr	5
software primer	5
nasba assay	5
annealing temperatures	5
microfluidic chips	5
lyophilized reagents	5
viral detection	5
blast search	5
throughput sequencing	5
viral diagnostics	5
sensitivity using	5
signal change	5
observed directly	5
therascreen assay	5
indian ocean	5
stock solution	5
virus titer	5
reticulum stress	5
clinical laboratory	5
tn transposase	5
diagnostic applications	5
cell development	5
single infected	5
national science	5
dna damage	5
hda systems	5
samples used	5
curl virus	5
table shows	5
synthetic biology	5
requires expensive	5
dna molecular	5
warmer lamp	5
ml buffer	5
autophagy pathway	5
synthbio review	5
visualized directly	4
envelope protein	4
size marker	4
frequently used	4
similar viruses	4
interacting protein	4
circulating strains	4
continuous flow	4
designed set	4
background interference	4
prm gene	4
genetic testing	4
low copy	4
amplification helicase	4
also thank	4
salmonella typhimurium	4
current knowledge	4
health systems	4
patient nasopharyngeal	4
also detected	4
viral burden	4
may require	4
repeated testing	4
detection procedure	4
nasopharyngeal samples	4
viral protein	4
early phase	4
primer binds	4
hereditary spastic	4
also included	4
may occur	4
primers hybridize	4
active form	4
genomic sequence	4
lysosomal proteases	4
cycle progression	4
synthesized dna	4
significantly higher	4
colorimetric readout	4
disposable pocket	4
reactive oxygen	4
japanese yam	4
spiral chip	4
chicken embryos	4
southern blot	4
several intrinsic	4
samples directly	4
family coronaviridae	4
eid ml	4
supplemental material	4
dual role	4
based extraction	4
avian virus	4
pcr without	4
expensive instruments	4
well pcr	4
either high	4
necator conidia	4
active covid	4
class i	4
target regions	4
also reported	4
like illness	4
mtor signaling	4
flow cabinet	4
conducted qlamp	4
colorectal cancer	4
strand invasion	4
often cumbersome	4
using isolated	4
preliminary results	4
high pure	4
exhibit increased	4
laboratory tests	4
surveillance studies	4
based nucleic	4
shenzhen luohu	4
diagnostic rt	4
cell membrane	4
chip release	4
identify infected	4
laser diode	4
virus stock	4
corresponding author	4
ppgv infection	4
recognize eight	4
identical reaction	4
reported lamp	4
lamp developed	4
health concern	4
single test	4
virus disease	4
chinese center	4
three major	4
step single	4
kisker biotech	4
cellular entry	4
set used	4
may serve	4
tm ii	4
respiratory distress	4
transcription factors	4
side effects	4
drug metabolism	4
amplifi ed	4
lower concentration	4
eukaryotic initiation	4
sample volume	4
dendritic cells	4
sensitive reverse	4
lamp employs	4
bubble plots	4
definitive diagnosis	4
important lessons	4
repurposed drugs	4
using uv	4
plays important	4
heat transfer	4
cell therapy	4
heating module	4
relatively conserved	4
pcr detected	4
inactivated nasopharyngeal	4
sequencing run	4
cap gene	4
bowel disease	4
regulated optical	4
daan gene	4
lysosomal membrane	4
loopamp rna	4
qpcr machine	4
detect target	4
polymerase large	4
cmnv plasmid	4
taq tm	4
sample treatment	4
autophagy deficiency	4
dna target	4
fold serially	4
technology co	4
validated using	4
various concentrations	4
air samples	4
carryover contamination	4
least times	4
two negative	4
care hospital	4
tetracapsuloides bryosalmonae	4
clinical characteristics	4
autophagy enhancer	4
rural areas	4
scored positive	4
gene segments	4
classical lamp	4
touchdown pcr	4
autophagy stimulation	4
circulating nucleic	4
obtained within	4
tests using	4
hairpin assembly	4
rheumatoid arthritis	4
small round	4
iii reverse	4
whole genome	4
sealing layer	4
autophagy protects	4
designed four	4
within one	4
symptomatic patients	4
detecting hcv	4
biomedical importance	4
stimulate autophagy	4
beclin overexpression	4
using different	4
nh cl	4
purification kit	4
influenza outbreaks	4
porcine pseudorabies	4
rna sample	4
quantitative lamp	4
equipped labs	4
aluminum foil	4
extension amplification	4
infection control	4
primer bip	4
one nasopharyngeal	4
warmstart rtx	4
lfd assays	4
sequence identity	4
subsequent rt	4
diagnostically useful	4
artery stenosis	4
performed according	4
sequences available	4
ndv qrt	4
extraction protocols	4
positive specimens	4
final products	4
washing buffer	4
naturally infected	4
linear curve	4
different stages	4
higher positive	4
fluorescence monitoring	4
activate autophagy	4
sporadic cases	4
adhesive foil	4
viral copy	4
time fluorometer	4
pathogenic role	4
sputum specimen	4
diseases autophagy	4
pigs infected	4
developed lamp	4
acid releasing	4
several inverted	4
labeled primer	4
culture supernatant	4
human health	4
death mechanisms	4
various clinical	4
preceding stroke	4
prv wild	4
reaction occurs	4
cell entry	4
template controls	4
assay sensitivity	4
bp fragment	4
strand rna	4
many countries	4
barr virus	4
superscript iii	4
two distinct	4
authors acknowledge	4
optical density	4
human transmission	4
throat swab	4
fully scalable	4
corona virus	4
nanopore target	4
mm naoh	4
ncov igm	4
standards ranging	4
aerosol contaminants	4
assay exhibited	4
diagnostic kits	4
samples detection	4
cation assay	4
first step	4
new york	4
every participant	4
within minutes	4
crick institute	4
regression analysis	4
circulating dna	4
like products	4
various pathogens	4
inoculum concentration	4
amplification buffer	4
amplifies dna	4
various rna	4
pbmcs isolated	4
clinically suspected	4
two groups	4
recent studies	4
processing steps	4
also suitable	4
many rt	4
internal primer	4
rtx reverse	4
therapeutic strategies	4
newly synthesized	4
assay established	4
maintaining homeostasis	4
rna polymerases	4
inhibitory effects	4
reported sample	4
biological samples	4
water buffalo	4
virus comparison	4
guangdong provincial	4
amplification curve	4
stroke patients	4
sickle cell	4
therapeutic effects	4
data processing	4
spike gene	4
either dna	4
six independent	4
qpcr positive	4
francis crick	4
using direct	4
synthetic viral	4
autophagy regulators	4
autophagic flux	4
mix consisted	4
fast detection	4
avian respiratory	4
current study	4
hairy nymphae	4
future prospects	4
conventional snp	4
biomedical research	4
encephalitis viral	4
igm antibodies	4
south india	4
lung injury	4
considered positive	4
widely applied	4
test lines	4
rna isolated	4
related coronaviruses	4
effusion samples	4
unless otherwise	4
commercial lamp	4
signal acquisition	4
initial stage	4
green channel	4
validated loop	4
used directly	4
lg ml	4
specimens obtained	4
studies showed	4
ethical committee	4
surgically resected	4
taq dna	4
initiation factor	4
cfu tube	4
fungicide applications	4
virus lysis	4
displaced strand	4
rna tissue	4
blind manner	4
coronavirus outbreak	4
positives due	4
coronavirus development	4
seen whether	4
inducing autophagy	4
assay evaluation	4
per cycle	4
dengue igg	4
positive sense	4
broad antiviral	4
several nucleic	4
acid isolation	4
high sequence	4
glycoprotein gene	4
different sizes	4
pcr plate	4
methods based	4
higher ct	4
tte uvrd	4
practical applications	4
accurately detect	4
lamp uses	4
different samples	4
fluorescent signals	4
treated blood	4
mg ml	4
target sequencing	4
standard strains	4
two regions	4
microfluidic platform	4
experimental procedures	4
necator inoculum	4
transparent adhesive	4
human dna	4
improved sensitivity	4
genetic disorders	4
genetically modified	4
hnb respectively	4
cold chain	4
high cost	4
additional advantage	4
bead stock	4
buffer containing	4
based diagnostic	4
reverse complement	4
short period	4
inflammatory bowel	4
also useful	4
service improvement	4
edwardsiella tarda	4
yielding inconsistent	4
ph change	4
economic losses	4
virus using	4
relatively small	4
respiratory infection	4
restriction enzymes	4
lamp kits	4
fl uorescence	4
emergent carotid	4
digital loop	4
suspected clinically	4
enhance autophagy	4
promotes apoptosis	4
bead capture	4
reference material	4
sophisticated equipment	4
autophagy suppresses	4
apoptosis induced	4
seven clinical	4
two patients	4
autophagic pathway	4
based probe	4
detect pathogens	4
flow control	4
polymorphism genotyping	4
ileumecaecal junction	4
amplifies nucleic	4
low sensitivity	4
rapid tests	4
isolation methods	4
nonstructural protein	4
dna dye	4
autophagy controls	4
zikv infections	4
west africa	4
antiviral drug	4
mass testing	4
handheld lamp	4
important roles	4
ct cutoff	4
threshold value	4
early identification	4
atg complex	4
beads packed	4
first evaluated	4
access holes	4
samples detected	4
strains prv	4
guanidinium thiocyanate	4
four negative	4
gold particles	4
neurological signs	4
suspected cases	4