PROFESSOR LIEBIG'S COMPLETE WOEKS ON CHEMISTRY. I/TTTTIV COMPKISING HIS AGRICULTURAL CHEMISTRY, OR ORGANIC CHEMISTRY IN ITS APPLICATION TO AGRICULTURE AND PHYSIOLOGY; ANIMAL CHEMISTRY, OR ORGANIC CHEMISTRY IN ITS APPLICATION TO PHYSIOLOGY AND PATHOLOGY; AND RESEARCHES ON THE MOTION OF THE JUICES OF THE ANIMAL BODY; TOGETHER WITH AN ACCOUNT OF THE ORIGIN OF THE POTATO DISEASE, WITH FULL DIRECTIONS FOR THE PROTECTION AND ENTIRE PREVENTION OF THE POTATO PLANT AGAINST ALL DISEASES. BY JUSTUS LIEBIG, M.D, PHD, F.R.S, M.R.I.A. PROFESSOR OF CHEMISTRY IN THE UNIVERSITY OP GIESSEN ; KNIGHT OP THE HES- SIAN ORDER, AND OF THE IMPERIAL ORDER OP SAINT ANN ', MEMBER OF THE ROYAL ACADEMY OF SCIENCES OF STOCKHOLM; CORRESPONDING MEM- BER OF THE ROYAL ACADEMIES OF SCIENCES OF BERLIN AND MUNICH; OF THE IMPERIAL ACADEMY OF ST. PE- TERSBURGH OF THE ROYAL INSTITUTION OF AMSTERDAM, ETC., ETC. " Every page contains a mass of information. I would earnestly advise all practical men, and all interested in cultivation, to have recourse to the book itself. The subject is vastly important, and we cannot estimate how much may be added to the produce of our fields by proceeding on correct principles." Loudon's Gardener's Magazine. " By the perusal of such works as these, the farmer need no longer be groping in the dark, and liable to mistakes ; nor would the most unnatural odium of farming by the book be longer existent. " In conclusion, we recommend these works to the agriculturist and to the horticulturist, to the amateur florist, and to the curious student into the mysteries of organic life, assured that they will find matter of interest and of profit in their several tastes and pursuits." Hovey's Magazine of Hor- ticulture. T. B. PETERSON, NO. 98 CHESTNUT STREET. ' KESEARCHES ON THE MOTION OF THE JUICES IN THE ANIMAL BODY; AND THE EFFECT OF EVAPORATION IN PLANTS. TOGETHER WITH AN ACCOUNT OP THE ORIGIN OF THE POTATO DISEASE; WITH FULL AND INGENIOUS DIRECTIONS FOR THE PROTECTION AND ENTIRE PREVENTION OF THE POTATO PLANT AGAINST ALL DISEASES. BY JUSTUS IIEBIG, M.D., PH.D., F.E.S., M.E.I.A. PROFESSOR OF CHEMISTRY IN THE UNIVERSITY OF GIESSEN. ILLUSTRATED WITH FIFTEEN FINE ENGRAVINGS. EDITED FROM THE MANUSCRIPT OF THE AUTHOR, BY WILLIAM GREGORY, M. D., PROFESSOR OF CHEMISTRY IN THE UNIVERSITY OF EDINBURG. T. B. PETERSON, No. 98 CHESNUT STREET. PREFACE. THE present little work contains a series of experiments the object of which is to ascertain the law according to which the mixture of two liquids, separated by a membrane, takes place. The reader will, I trust, perceive in these researches an effort to attain, experimentally, to a more exact expression of the conditions under which the apparatus of the circulation acquires all the properties of an apparatus of absorption. In the course of this investigation, the more intimate study of the phenomena of Endosmosis impressed on me the conviction that, in the organism of many classes of animals, causes of the motion of the juices were in operation, far more powerful than that to which the name of Endos- mosis has been given. The passage of the digested food through the membranes of the intes- tinal canal, and its entrance into the blood ; the passage of the nutrient fluid outwards from the blood vessels, and its motion towards the parts where its constituents acquire vital properties, these two fundamental phenomena of organic life cannot be explained by a simple law of mixture. The Experiments described in the following pages will, perhaps, be found to justify the conviction that these organic movements depend on the transpiration and on the atmospheric pressure. The importance of the transpiration for the normal vital process has, indeed, been acknowledged by physicians ever since Medicine had an existence ; but the law of the dependance of the state of health on the quality of the atmosphere, on its barometric pressure, and its hygrometric condition, has been hitherto but little investigated. By the researches contained in my examination of the constituents of the juice of flesh, as well as by those described in the present work, the completion of the second part of my Animal Chemistry has been delayed ; but I did not consider myself justified in continuing that work until I had examined the questions suggested by, and connected with those researches. DR. JUSTUS LIEBIG. GIESSEN, February, 1850. (5) EDITOR'S PREFACE. IN the Editor's Preface to Baron Liebig's " Researches on the Chemistry of Food," in which the Author gave the results of his investigation into the constituents of the juice of the flesh, I mentioned that Baron Liebig had been led to study the subject of Endosmosis experimentally. The results of this investigation are contained in the following pages ; and the reader will, I trust, be satisfied that the motions of the animal juices depend on something more than mere Endosmosis or Exosmosis, and that the pressure of the atmosphere, as well as its hygrometric state, by influencing tho transpiration from the skin and lungs, are essentially concerned in pro- ducing these motions. At the same time, the present work is to be regarded, not as exhausting the subject, but, on the contrary, as only pointing out the direction in which inquiry is likely to lead to the most valuable results. While it is proved that the mechanical causes of. pressure and evapora- tion, and the chemical composition of the fluids and membranes, have a more direct, constant, and essential influence on the motion of the animal fluids, and, consequently, on the state of the health, than has been usually supposed, it is evident that very much remains to be done in tracing that influence under the ever varying circumstances of the animal body, and in applying the knowledge thus acquired to the purposes of hygiene and therapeutics. But it is equally obvious, that the above-mentioned mechanical and chemical causes are not alone sufficient to explain the phenomena of animal life, since they are present equally in a dead and in a living body ; so that while every advance in physiology enables us to explain more facts on chemical and mechanical principles, something always (3) iv EDITOR'S PREFACE. remains, which, for the present, is beyond our reach, and which may for- ever remain so. However this may be, the facts established in this and in the preceding work of the Author have very materially extended the application of the well-known laws of physics and of chemistry to physio- logy, and have also furnished a number of the most beautiful instances of that infinitely wise, but exquisitely simple adaptation of means to ends, which characterizes all the works of the omnipotent Creator ; but which is no where more admirably displayed, than in the arrangements, imperfectly known as they hitherto are, by which life is maintained. In connection with the Author's remarks on the effects of evaporation in plants, and the consequences of its suppression, and with his opinions as to the origin of the potato disease, I beg to refer the reader to the Appendix for a very ingenious and apparently well founded plan for the protection of the potato plant against the terrible scourge under which it has lately suffered. The views of Dr. Klotzsch, the author of this plan, as to the nature of the disease, coincide remarkably with those of Baron Liebig, as explained in the present work. WILLIAM GREGORY. EBIITBUBGH, 3d March, 1 850. CONTENTS. PAGE On the phenomena accompanying the mixture of two liquids separated by a mem- brane 9 Relation of porous bodies -to water and other liquids 9 The moistening of porous bodies depends on capillary attraction 10 Pressure required to cause liquids to pass through membranes 11 The pressure varies with different liquids 11 The absorbent power of the membrane has a share in the effect 11 Action of brine, oil, alcohol, &c., on moist membranes 12 Cause of the shrivelling of membranes when strewed with salt ."..... 13 Animal tissues are permeable to all liquids 14 Saline solutions, alcohol, &c., mix with water through membranes 15 Change of volume when two dissimilar liquids mix through a membrane ; Endosmosis 15 This change of Tolume does not depend alone on the different densities 15 Phenomena of the mixture of two liquids through a membrane 16 The mixture is the result of chemical attraction 18 Chemical attraction is every where active 19 Examples. Crystallization 19 Action of solids on dissolved matters 19 Laws of the mixture of two dissimilar liquids 21 Effect of the interposition of a membrane 22 The change of volume in two liquids which mix through a membrane is the result of chemical affinity modifying capillary attraction 23 Effect of evaporation on liquids confined by membranes 24 Views of MAGNUS on Endosmosis 24 Remarks on his theory 24 The nature of the membrane has an important influence 25 Unequal attraction of membranes for different liquids 26 The action of two liquids, separated by a membrane, is equivalent to pressure, un- equal on opposite sides 27 Causes which influence the mixture of two liquids separated by a membrane 29 These causes produce, in the animal body, absorption of the fluids of the intestines into the blood 30 Effects of drinking water and saline solutions of different strengths 30 Influence of the cutaneous evaporation on the motion of the animal juices 31 (7) 8 CONTENTS. PACK Experiments 32 Influence of the atmospheric pressure 33 Water passes through membranes more easily than air does 34 Experiments on evaporation through membranes 34 Importance of the cutaneous transpiration 35 By it the fluids acquire a motion towards the skin and lungs 35 Effects of dry and moist air, and of elevation, on the health 35 Causes of the efflux of sweat 36 Fishes die in air, because the due distribution of the fluids is prevented 36 Experiments of HALES on the motion of the sap in plants 36 This motion is caused by evaporation 37 Force with which the sap rises 37 The atmospheric pressure is the active force 38 The sap absorbs gases 38 The evaporation supplies food to the plant 38 Influence of suppressed evaporation on hop vines 39 Observations of HALES on the blight in hops, &c 39 Fire-blasts in hops 39 HALES recognized the influence of evaporation on the life of plants 39 The origin of the potato disease is probably similar to that of the blight in hops. . . 40 The disease long known 40 It is due, not to a degeneration of the plant, but to a combination of external cir- cumstances 40 It is connected with the weather, and particularly with the temperature and hygro- metric state of the atmosphere 41 The life of plants is dependent chiefly on four external causes 41 Only one of which, namely, the quality of the soil, is in the power of the agriculturist 41 Effects of suppressed evaporation 41 The fungi and putrefaction follow the death of the plant 41 Observations of HALES on the rise of the spring sap in perennial plants 41 Views of DUTROCHET 42 Objections to these views 42 The cause of the rise of the sap is transient, and depends on external influences. . . 42 It exists, not merely in the spongioles, but in all parts of the plant 42 Experiments of HALES 43 His conclusions 43 Gas is given off by the sap 44 The rise may therefore be due to the disengagement of gas 44 The gas is probably carbonic acid 44 APPENDIX. Account of a plan proposed by Dr. KLOTZSCH, of Berlin, protecting potato plants from disease 45 This plan published by authority of the Minister of the Interior of Prussia, on the favorable report of the President of the College of Rural Economy at Berlin. ... 47 Conditions on which the reward, claimed for his plan, if found effectual, by Dr. KLOTZSCH, has been granted 47 ON THE PHENOMENA ACCOMPANYING THE MIXTURE OF TWO LIQUIDS SEPARATED BY A MEMBEANE. THE constituents of the food, which have assumed a soluble form in the alimentary canal, are thereby endowed with the property of yielding to the influence of every cause which, in acting on them, tends to change their place or the position which they occupy.* They are conveyed into the blood vessels, and from thence are distributed to all parts of the body. The movement and distribution of these fluids, and of all the substances dissolved in them, exclusive of the mechanical cause of the contraction of the heart, by which the circulation of the blood is effected, depend, 1, on the permeability of the walls of all vessels to these fluids ; 2, on the pressure of the atmosphere ; and 3, on the chemical attraction which the various fluids of the body exert on each other.! The motion of all fluids in the body is effected by means of water : and all parts of the animal system contain, in the normal state, a certain amount of water. Animal membranes, tendons, muscular fibres, cartilaginous ligaments, the yellow ligaments of the vertebral column, the cornea, transparent and opaque, &c., all contain, in the fresh state, more than half their weight of water, which they lose, more or less completely, in dry air.J On the presence of this water depend several of their physical properties. The fresh, opaque, milk-white cartilagesof the ear become, when dried, translucent, and acquire a reddish yellow color. Tendons, when fresh, are in a high degree flexible and elastic and possess a silky lustre, which they lose when dried. By the same loss of water they become, further, hard, horny, and translucent, and when bent, split into whitish bundles of fibres. The sclerotic coat is milk-white when fresh, and becomes transparent by desiccation. When these substances, after having lost, by drying, a part of the properties which they possess in the fresh state, are again placed in contact with pure water, they take up, in 24 hours, the whole original amount of water, and recover per- fectly those properties which they had lost. The opaque cornea, or sclerotic coat, which had become transparent by desiccation, again becomes milk-white, while the transparent cornea, which had been rendered opaque by drying, now becomes again transparent. The tendons, which, when dried, had become horny, hard, and translucent, now again become flexible and elastic, and recover their silky * The food becomes soluble, and the fluids of the body are sent to all parts. t General causes of their motion. j Relation of animal tissues to water. a (9) 10 MOTION OF THE JUICES OF THE ANIMAL BODY. lustre. The fibrine and the cartilages of the ear, which desiccation had rendered horny and transparent, again become milk-white and elastic. The power which the solids of the animal body possess of taking up water into their substance, and of being penetrable to water, extends to all fluids allied to water, that is miscible with it.* In the dried state, the animal solids take up fluids o.f the most diverse natures, such as fatty and volatile oils, ether, bisulphuret of carbon, &c. This permeability to fluids is possessed by animal tissues in common with all porous bodies ; and no doubt can be entertained, that this property is determined by the same cause which produces the ascent of fluids in narrow tubes, or -in the pores of a sponge ; phenomena, which we are accustomed to include under the name of capillary action. One condition, essential to the permeability of porous bodies for fluids (or their power of absorption), is their capability of being moistened ; or the attraction which the particles of the fluid and the walls of the pores or tubes have towards each other.t A second condition is the attraction which one particle of the fluid has to another. We have no means of estimating the absolute size of the particles or molecules of a fluid, such as water, but they are certainly infinitely smaller than the measurable diameter of a tube, or of the pores of a porous body. It is obvious, therefore, that in the interior of a capillary tube or pore, filled with a fluid, only a certain number of the fluid molecules are in contact with the walls of the tube, and attracted by them ; while in the middle of the tube, and from thence towards its parietes, fluid molecules must exist which only retain their place in virtue of the attraction which the molecules, attracted by the parietes, exert on those not so attracted ; that is, by the cohesive altraction of the fluid. Liquids flow out of capillary tubes, which are filled with them, only when some other force or cause acts, because capillary attraction cannot produce motion beyond the limits of the solid body which determines the capillary action. The penetration of a fluid into the pores of a porous body, is the result of capillary attraction ; its expulsion can be affected by a mechanical pressure ; and may be accelerated by increasing this pressure, and by all such causes as diminish the mutual attraction of the fluid molecules, or the attraction of the walls of the pores for those molecules. The condition most favorable to the passage of a fluid through the pores of a porous substance under pressure, is when one fluid molecule can be displaced so as to glide away over another. The slightest pressure suffices to expel the displaceable particles of water from a sponge ; a higher pressure is required to express the same fluid from bibulous paper ; and a pressure much higher still is necessary in order to cause water to flow out of moist wood.J We may form some idea of the force with which porous organic substances, such as dry wood, absorb and retain water, if we remember, that by inserting of wedges of dry wood in proper cuts, and subsequently moistening them, rocks may be split and fractured. When we compare with the properties just enumerated, which belong to all porous bodies, those properties which are observed in animal substances under the same circumstances, it appears plainly that these animal substances have pores in certain directions ; although these openings are so minute that they are not, in the case of most tissues, perceptible, even with the aid of the best microscopes. It has been mentioned that tendons, ligaments, cartilages, &c., contain, in the fresh state, a certain amount of water, which, according to all experiments made on the subject, is invariable ; and that several of their properties depend on the presence of this water.|| (CHEVREUL.) When these substances, wrapped in bibulous paper, are subjected to a powerful pressure, a certain proportion of this water is expelled. Fresh and flexible vessels lose, in this way, 37.6 per cent., and the yellow liga- ments of the vertebras lose 35 per cent, of water. This property, namely, that of losing water under pressure, is only found in porous substances. It is obvious that by pressure, that is, by diminution of the size of the pores, only that portion of * The tissues absorb other fluids. t The moistening of porous bodies. ^Prodigious force with which porous bodies absorb water. $ Animal tissues are porous. || Amount of water expelled by pressure from tissues. ABSORBENT POWER OF MEMBRANES. 11 water can be pressed out which is not retained by chemical attraction.* It is in the highest degree worthy of notice, that this water, not chemically combined, seems to have the greatest share in the properties which these animal substances possess in the fresh state, for the pressed tendons and yellow ligaments become transparent; the former lose their flexibilty, the latter their elasticity ; and if laid in water, they recover these properties perfectly. In the pores of a porous substance, the fluid molecules are retained by two kinds of attraction, namely, by the affinity which is exerted between the walls of the pores and the molecules of the fluid, and by the cohesion which acts between the molecules of the fluid itself. It would appear as if the molecules of water were thus brought into different states, and this seems to be the cause of the differences observed in the properties of these animal substances when they contain different proportions of water. Fig- t If the wide opening of the tube, Fig. 1, be tied over with a por- tion of bladder, and water poured into the wide part of the tube, as far as the mark a, we shall find that, when mercury is poured into the upright narrow part of the tube, to a certain height, the whole external surface of the bladder becomes covered with minnte drops, which, if the column of mercury be made a few lines higher, unite, so as to form large drops. These continue to flow out uninter- ruptedly, if mercury be added, so as to keep the column at the same height, till at last the wide part of the tube is emptied of water and filled with mercury. Solution of salt, fat oil, alcohol, &c., behave exactly as water does ; under a certain pressure these fluids pass through an animal mem- brane, just as water does through a paper filter. The pressure required to cause these liquids to flow through the pores of animal textures depends on the thickness of the membrane, as well as on the chemical nature of the different liquids. Through ox-bladder, T ^th of a line (yj^th of an inch) thick, water flows under a pressure of 12 inches of mercury 4 A saturated solution of sea salt requires from 18 to 20 inches ; and oil (mar- row oil) only flows out under a pressure of 34 inches of mercury. When the membrane used is the peritoneum of the ox, 2 ^th of a line, (jl^th of an inch) in thickness, water is forced through it by 8 to 10 inches, brine by 12 to 16 inches, oil by 22 to 24 inches, and alcohol by 36 to 40 inches of mercury. The same membrane from the calf, gjgth of a line (y^g^d of an inch) in thick- ness, allows water to pass through under the pressure of a column of water 4 inches high ; brine passes under a pressure of 8 to 10 inches of brine, and oil under a pressure of 3 inches of mercury. In making experiments of this nature, we observe that, after they have continued for some time, the pressure required to force the liquid through the membrane does not continue equal. If during the first 6 hours a pressure of 12 inches of mer- cury were necessary, we often find that after 24 or 36 hours, 8, or even 6 inches will suffice to produce the same effect, obviously because by long-continued con- tact with water, the membrane undergoes an alteration, in consequence of which the pores are widened. From these experiments it appears, that the power of a liquid to filter through an animal membrane bears no relation to the mobility of its particles ; for under a pressure which causes water, brine, or oil to pass through, the far more mobile alcohol does not pass. The capacity of the animal membrane for being moistened by, and its power of absorbing, the liquid, have a certain share in producing the result of its filtration through the membrane. || The following table will show this fact : * The portion of water not chemically combined, has the greatest share in the properties of the tissues. j- Pressure required to cause water and any other liquids to pass through membranes. T The pressure varies with different liquids. $ The passage of liquids through membranes not in proportion to their fluidity. I The absorbent power of the membrane for the liquid has a share in the effect. 12 MOTION OF THE JUICES OF THE ANIMAL BODY. 100 parts, by weight, of dry ox-bladder, take up in 24 hours, of pure water 268 volumes saturated solution of sea salt (brine) . . . 133 alcohol of 84 per cent 38 oil of marrow* 17 100 parts, by weight, of ox-bladder, take up in 48 hours, of pure water 310 parts by weight of a mixture of water and | brine .... 219 * ,, * 235 * * 288 i alcohol i 60 ,, I -.181 * I 290 100 parts of dry pig's bladder take up in 24 hours, of pure water 356 volumes brine 159 , oil of marrow 14 From these experiments it appears that the absorptive power of animal mem- branes for different liquids is very different. Of all liquids, pure water is taken up in the largest quantity ; and the absorptive power for solution of salt diminishes in a certain ratio as the proportion of salt increases. A similar relation holds between the membranes and alcohol ; for a mixture of alcohol and water is taken up more abundantly the less alcohol it contains. 1 (!) In regard to this property, membranes differ in no respect from other animal textures, as was long ago proved by Chevreul. This distinguished philosopher found that the following substances absorbed, in 24 hours, of water, brine, and oil, Cubic Centimetres C. C. C. C. Water. Brine. Oil. 100 grammes of cartilage of the ear 231 125 100 tendons 178 114 8.6 100 yellow ligaments of spine . . 148 30 7.2 100 cornea 461 370 9.1 100 cartilaginous ligaments .... 319 3.2 100 dry fibrine absorbed 301 of water and 148 of alcohol of 69 per cent. (Liebig.) 100 184 parts by weight or 154 by volume of brine. Animal membranes do not acquire, by absorbing alcohol or oil, those properties which they exhibit when saturated with water.! A dried bladder continues hard and brittle in alcohol and oil ; its flexibility is in no degree increased by absorbing these liquids. When tendons, ligaments (CHEVREUL,) the yellow liga- ments of the spine, or bladder, saturated with oil, are placed in water, the oil is completely expelled, and they take up as much water as if they had not previously been in contact with oil. It has been mentioned, that 100 parts of animal membrane (dry ox-bladder) absorb in 24 hours 268, in 48 hours 310 volumes of water, and only 133 of saturated solution of salt. It follows, of course, that when the bladder, saturated with water by 48 hours' contact, and well dried in bibulous paper, without pressure, to remove superfluous water, is strewed with salt, there is formed, at all points where salt comes in contact with the water filling the open pores, a saturated solu- * Absorption of different liquids. t Effects of oil, salt, &c., on membranes when dry, and when in the moist state. MEMBRANES SATURATED WITH WATER. 13 tion of salt, the salt contained in which diffuses itself equally in the water of the bladder. Of the 310 volumes of water which become thus saturated with salt, only 133 volumes are retained in the bladder ; and in consequence of this diminu- tion of the absorbent power of the bladder for the brine, 177 volumes of liquid are expelled, and run off in drops from the surface of the bladder. Membranes, fibrine, or a mass of flesh, behave exactly in a similar manner when in contact with alcohol. If placed in alcohol in the fresh state, that is, when they are thoroughly charged with water, there are formed, at all points where water and alcohol meet, mixtures of the two, and as the animal texture absorbs much less of an alcoholic mixture than of pure water, more water is, of course, expelled, than alcohol taken up. 9-17 grammes of bladder, fresh, that is saturated with water (in which are con- tained 6'95 grammes of water, and 2-22 of dry substance,) when placed in 40 cubic centimetres of alcohol, weigh, at the end of 24 hours, 4-73 grammes, and have, consequently, lost 4-44 grammes.* In the 4-73 grammes which remain, are 2-22 grammes of dry bladder, and, of course, 2-51 grammes of liquid. If we assume that this liquid has the same composition as the surrounding mixture (which is found to contain 84 parts of alcohol to 16 of water,) it will consist of 2-11 grammes of alcohol and 0-40 of water; and consequently, of the 6-95 gram- mes of water originally present, 6-45 grammes have been expelled, and replaced by 2-11 grammes of alcohol. For 1 volume of alcohol, therefore, retained by the bladder, rather more than 3 volumes of water have been expelled from it. t Since, in this case, so much more water is expelled than is taken up of alcohol, the first result is a shrinking of the animal substance.^) If the bladder could take up or absorb equal volumes of brine and water, or of alcohol and water, then when the fresh bladder was strewed with salt, or laid in alcohol, the volume of the absorbed liquid would be unaltered, and an equal volume of saline solution, or of diluted alcohol, would be retained by the animal tissue. But since the absorbent power of the tissue for water is diminished by the addition of salt, or of alcohol, it follows plainly, that a certain quantity of water must be expelled as soon as its character is changed by the addition of one of these substances. The relation of bladder, fibrine, and other animal substances, when saturated with water, to alcohol and brine, proves, that the shrinking (diminution of volume) of these tissues does not depend on a simple abstraction of water in virtue of the affinity of alcohol and of salt for that liquid ; for it is quite certain that the attrac- tion of alcohol to water, and that of water to alcohol, are respectively equal.J The attraction of the water within the tissue for the alcohol without, is just as strong as the power of the alcohol without to combine with the water within. Less alcohol is taken up, and more water given out, because the animal tissue has less attraction for the mixture of alcohol and water than for pure water alone. The alcohol with- out becomes diluted, the water within becomes mixed with a certain proportion of alcohol, and this exchange is only arrested when the attraction of the water for the animal tissue, and its attraction for alcohol, come to counterpoise each other. ? If we regard a piece of skin, or bladder, or fibrine as formed of a system of capil- lary tubes, the pores or minute tubes are, in the fresh state, filled with a watery liquid, which is prevented from flowing out by capillary attraction. But the liquid contained in these capillary tubes flows out of them as soon as its composition is altered by the addition of salt, alcohol, or other bodies. ( } ) Fibrine and other animal matters exhibit results quite similar to those obtained with bladder. 26'02 grammes of fibrine saturated with water (containing 6'48 grammes of dry fibrine and 19'54 of water) were reduced, in 45 grammes of absolute alcohol, to 16-12 grammes, losing, therefore, 9'90 grammes. Admitting the absorbed liquid to have the composition of the unabsorbed residue (70 per cent, of alcohol,) it appears, that for 1 volume of alcohol absorbed by fibrine rather more than 2 volumes of water are separated. * Amount of water expelled from bladder by alcohol. t Moist membranes shrink when strewed with salt, or placed in alcohol. + The cause of this is the less affinity of the tissue for alcohol, &c., than for water. 14 MOTION OF THE JUICES OF THE ANIMAL BODY. If we lay together, one over the other, two portions of bladder, saturated with solution of salt of sp. g. 1-204, and over the upper one another piece of bladder of equal size, saturated with water, and if we allow them to remain thus, without pressure, we find, after some minutes, when the two pieces saturated with solution of salt are separated, that drops of saline solution appear between them, of which no trace could previously be perceived. If the piece of bladder saturated with water contained 5 volumes of water, and the next piece 3 volumes of saline solution, there must be produced, by the mixture of both, 8 volumes of diluted saline solution, of which each piece of bladder must contain one half, or 4 volumes, if the absorbent power of the portion saturated \vith the original saline solution were increased by the addition of water in the same ratio as the absorbent power of the portion satu- rated with water was diminished by the addition of salt. The saline liquid would have given up Is volume of saline solution to the other, and would have received from it 2 volumes of water. In this case, the mixture in the two upper pieces of bladder would occupy the same space which its constituents, water and saline solution, occupied in each singly. But the efflux of the liquid towards the third or lowest piece of bladder saturated with saline solution, proves, that the two upper pieces retain a smaller volume of the mixture newly formed in their pores, than the one piece absorbed of water alone, and the other of saline solution alone. The power of retaining water is diminished by the addition of salt to the bladder saturated with water ; liquid is expelled ; but by the addition of this water to the bladder moistened with saline solution, the absorbent power of this piece of blad- der is increased, not in the same ratio according to which the proportion of salt is diminished, but in a less ratio. The experiments above described show that the attraction of the porous sub- stances for the water which they have absorbed does not prevent the mixture of this water with other liquids. The permeability of animal tissues to liquids of every kind, and the miscibility of the absorbed liquids with others which are brought in contact with the tissues, may be demonstrated by the simplest experiments.* If we moisten one side of a thin membrane with ferrocyanide, of potassium, and the opposite side with chloride of iron in solution, we perceive in the substance of the membrane a spot of Prussian blue immediately deposited. (Jon. MULLER.) All fluids which, when brought together, suffer a change in their nature or in their properties, exhibit, when only separated by an animal membrane, exactly analogous results ; they mix in the pores of the membrane, and the decomposition commences in its substance. If we tie up one end of a cylindrical glass tube with bladder, and fill it to the height of 3 or 4 inches with water or strong brine, neither the water nor the brine flows out through the pores of the bladder under this slight pressure. But if we leave the tube containing brine exposed to evaporation in the air, the side of the bladder exposed to the air is soon covered with crystals of salt, which gradually increase, so as to form a thick crust.t It is obvious that the pores of the bladder become fluid with brine ; that, on the side exposed to the air, the water evaporates ; its place is supplied by fresh brine, and the dissolved salt is deposited at the external minute openings of the pores, in the form of crystals. If the tube be filled originally with dilute saline solution, the crust of salt is not formed on the outer surface of the bladder until the solution in the tube has reached, by evapora- tion, the maximum of saturation. Before this takes place, we can perceive in the tube, if we set the liquid in motion, two strata, a heavier and a lighter, the latter swim- ming on the former. When these strata can no longer be observed, the liquid is in every part saturated with salt ; and now, by further evaporation, crystals are deposited on the outer surface of the bladder. This last circumstance proves that the amount of salt in the liquid is uniformly distributed from below upwards, from the specifically heavier to the specifically lighter part. If we immerse the tube closed with bladder, and filled with saline solution, in pure water, the latter acquires the property of precipitating nitrate of silver, even * Animal tissues are permeable to liquids of every kind, which act on each other in the sub- etance of the tissues. t Deposition of salt on the outside of bladder from brine on the inside. MIXTURE OF THE LIQUIDS. 15 when the contact has lasted only the fraction of a second.* The brine filling the open pores of the membrane mixes with the pure water, and the latter acquires a certain quantity of salt. In like manner, the pure water acquires a saline impregnation, when it is placed in the tube instead of brine, and the outer surface of the bladder is placed in con- tact with solution of salt. When the tube, closed with bladder, and filled with brine, is left for a long time with the closed end immersed in pure water, the amount of salt in the latter increases, while that of the brine diminishes, till at last the two liquids, separated by the bladder, contain the same relative proportions of salt and water. If the liquid in the tube contain, dissolved, other substances which give to it properties different from those of pure water, and if these solutions be miscible with water, the mixture of them with the water takes place exactly as in the case of brine.t This is true of saline solutions of every kind ; of bile, milk, urine, serum of blood, syrup, solution of gum, &c., on the one side, and pure water on the other. The concentrated liquid loses, the water or diluted liquid gains, in regard to saline impregnation. If we fill the tube with water, and place it in a vessel with alcohol, the water becomes charged with alcohol, while the alcohol becomes diluted with water. There is observed, in these circumstances, that is, when two dissimilar liquids, separated by a membrane, mix together, a phenomenon of a peculiar kind ; namely, in most cases a change of volume in both liquids, while the mixture goes on. The one liquid increases in bulk, and rises ; the other diminishes in the same degree, and consequently sinks below its original level. This phenomenon of mixture through a membrane, accompanied with change of volume, has been distinguished by DUTROCHET, under the name of ENDOSMOSIS and EXOSMOSIS ; endosmose is the name given when the volume increases exos- mose, when it diminishes. Very generally, however, we attach to these terms the idea of the unknown cause or group of causes which, in the given case, produce the change of volume ; in the same sense as that in which the term capillary action includes the causes which determine the ascent of liquids in narrow tubes. In all cases* the increase in volume of the one liquid is exactly equal to the decrease in volume of the other, after making allowance for the contraction which the liquids undergo by simple mixture (as in the case of alcohol and water, oil of vitriol and water, &c.,) as well as by evaporation. The unequal concentration, or the unequal density of the two liquids, has a decided influence on the rapidity with which the change of volume takes place ; but this cannot be viewed as the cause of that phenomenon. In most cases the denser liquid increases in volume, in others the reverse occurs. When, for example, the tube contains brine, and the outer vessel pure water, the brine, that is the denser liquid, increases in volume ;|| but when the tube con- tains water, and the outer vessel alcohol, the water, that is, the denser liquid, diminishes in volume. With regard to the mixture of the liquids, the bladder takes a distinct share in the process, inasmuch as it has pores, through which the two liquids are brought in contact. With reference to the porosity of the bladder, the rapidity of the mixture of the two liquids is directly proportional to the number of particles, which, in a given time, come into contact ; it depends also on the surface (the size of the mem- brane,) and on the specific gravity of the liquids. The influence of extent of surface on the time required for mixture requires no particular elucidation ; that of the unequal specific gravity is rendered evident by the following experiments.^ * Saline solutions pass very rapidly through bladder. fThe same is true of bile, milk, urine, serum, &c. t Change of volume when two dissimilar liquids mix through a bladder. $ Endosmosis and Exosmosis. (The change of volume does not depend alone on the relative density of the liquids. f Influence of the unequal density of the two liquids, when the lighter liquid is above the membrane* 16 MOTION OF THE JUICES OF THE ANIMAL BODY. If the bent tube a b (Fig. 2,) one end of which is tied over with bladder, and the other open, be filled with brine colored blue^ 1 ) and if pure water be placed in the tube c, there is soon perceived under the bladder a colorless or nearly colorless stratum of liquid, which continues for hours to float in the same place. If the bent tube a b be filled with colorless brine, while c is filled with pure water colored blue, there is found, after a time, above the bladder, a colorless or nearly colorless stratum of liquid. It appears from this, that an exchange of both liquids goes on through the substance of the bladder ; in the first experiment color- less water passes from the tube c to the colored brine in the tube a b ; in the second, colorless brine passes from the tube a b to the colored water in the tube c. It is obvious that the brine in the tube a b, which is in contact with the bladder, becomes diluted by the addition of water from the tube c ; but this diluted brine is specifically lighter than the original brine which is below it, and remains therefore floating at its surface. On the other hand, the water in the tube c, when mixed with brine from the tube a b, becomes heavier, than the pure water, and rests, therefore, on the upper surface of the bladder, or that which is turned towards the water. Hence it follows, that from the moment when these two strata have been formed above and below the bladder, neither concentrated brine nor pure water comes any longer in contact with the bladder. From the bladder downwards, in the tube a b are strata of liquid, containing successively more salt ; from the bladder upwards in the tube c are strata containing successively more water. In the beginning of this experiment we observe v that the volume of the water and of the brine changes in both tubes ; the liquid in the limb b rises from 1 to 2 lines ; but as soon as the strata above mentioned have been distinctly formed above and below the bladder, hardly any further rise is perceptible, although the mixture of the liquid proceeds, and the water in c becomes constantly more charged with salt, while the brine in a b loses salt. If we reverse the positions of the two liquids in the apparatus Fig. 2, or what is simpler, if we close with bladder a tube 1 centimetre (^ ff ths of an inch) wide, fill it with brine, and immerse the end closed with the bladder in a wider vessel filled with pure water, giving to the tube an inclination of about 45, we may observe (most distinctly when both liquids contain some fine particles of indigo suspended) in both liquids a continual motion.* We see in the tube (Fig. 3) a current of liquid rising from the bladder in the direction of the arrow, and flowing down again on the opposite side. A similar circulation is observable in the vessel of water. If the tube , with brine, is about 2 centimetres (fths of an inch) wide, and if we support it vertically in the vessel b of water, the motion proceeds from the middle, and in both the tube and the vessel we perceive currents in opposite directions. (Fig. 4.) These currents hardly require explanation. To the brine in the tube a, pure water passes through the bladder ; there is formed above the bladder a mixture containing less salt, and therefore specifically lighter than the brine ; this mixture rises, and the denser brine descends to occupy its place. On the other hand, the pure water receives through the bladder salt, and becomes thereby specifically heavier; while it sinks to the bottom of the vessel, its place is supplied by water containing Fig. 3. Fig. 4. (') For this purpose it is best to take a solution of indigo in sulphuric acid, diluted, When the heavier liquid is above the membrane. MIXTURE OF TWO LIQUIDS THROUGH A MEMBRANE. 17 Fig. 5. less or no salt, and therefore specifically lighter, which again comes in contact with the bladder. As long as the motions just described are perceptible, we observe a constant increase in the volume of the brine in the tube a (Fig. 4,) or a diminution in the volume of the pure water in the vessel b. When the motions cease, the rise of liquid in the tube is arrested, and when this takes place, the two liquids are found to possess almost exactly the same specific gravity. When the two strata of liquid on either side of the bladder are little different in composition (as soon comes to pass in the experiment (Fig. 2) where the saline contents of the liquid which fills the pores of the bladder can hardly vary from that of the next stratum,) the mixture of the liquids takes place, but without further change of volume. But when an exchange of the mixtures on the opposite sides of the bladder can occur in consequence of their different specific gravity, and when a continued difference between the strata on opposite sides of the bladder is thus determined, then, so long as (in the case of brine and water, for example) one side of the bladder is in contact with a concentrated, the other with a more diluted solution, the change of volume in the two liquids continues. As appears from these experiments, the change of volume depends on a difference in the character of the two liquids which are connected through the bladder ; and the time during which change of volume occurs is in direct proportion to the time during which this difference in character subsists. The greater the difference in character and composition between the liquids, and the more rapidly this difference is renewed by the exchange between the strata in contact with the opposite sides of the bladder, the more rapidly does the one liquid increase, and the other diminish in volume. The following apparatus is very convenient for measuring the change in volume, caused by the mixture of two liquids separated by a membrane.* The tubes a and 6, (Fig. 5) are of equal width, and are best takem from the same tube ; a is closed with bladder, and filled up to a cer- tain point with the liquid whose increase in volume is to be determined. It is then fitted by means of a good cork into the wider tube c, which contains distilled water, care being taken to exclude all air bubbles. At d lies a small lead drop, which acts as a valve in shutting the opening of the capillary tube connecting c with b. Pure water is now poured into 6, and in order to keep in equili- brium the lead drop at d, rather more water is added than exactly suffices to bring the liquids to the same level in both tubes. The liquid in a increases in volume, and the height to which it rises may be read off by means of any division into equal parts by measure ; the level of the liquid in b sinks in an equal ratio. If we keep the liquid in b, by the addition of fresh water, at the original level, and if we ascertain the weight of the added water, by pouring it out of a dropping bottle, and determining the loss of weight in the dropping bottle, we learn, at the same time, the weight and the volume of the water which has risen from c into a. This apparatus admits, of course, of a number of variations and improvements. I have employed it to determine the relation between brine and water, under the circumstances just described. It appeared, among other things, that when the tube a is filled with saturated solution of sea salt, the volume of the liquid increased by nearly 'one half; that is, 200 volumes of such a solution increased to 300. These determinations are, however, not the object of the present investigation, and therefore I pass them over entirely. The following arrangement, (Fig. 6) will probably be found preferable to the one just described, in many cases. Its construction depends on the observation, that for the phenomenon itself, and for the result of the experiment, it is entirely a and after adding subacetate of lead as long as sulphoindigotate and sulphate of lead are precipitated, to separate the precipitate by filtration and dry up the filtered liquid in the water bath. A mere trace of the blue residue suffices to color blue large masses of liquid. * The change in volume may be measured. 3 18 MOTION OF THE JUICES OF THE ANIMAL BODY. matter of indifference whether the tube be closed with a single, double, or treble layer of bladder^ 1 ) For experiments- on very thin membranes which are permeable to liquids under a very low pressure, the apparatus (Fig. 5) is obviously better adapted. For the explana- tion of the phenomenon we have to distinguish 1. The mixture of different liquids. 2. The change in their volume. As to the mixture of two liquids of dissimilar nature and characters, this always depends on a chemical attraction.* In a mixture of alcohol and water, or of brine and water, there is in every part the same proportion of particles of alcohol and water, or of salt and water. If in the former, the lighter particles of alcohol lying at the bottom of the vessel were not retained, in the place and arrangement which they occupy, by the surrounding particles of water, they would undoubtedly rise towards the surface. In like manner, the particles of salt in the brine are sustained and prevented from sinking by the lighter particles of water which surround them. Without an attraction, which all the particles of alcohol or of salt must have towards all the particles of water, or all the particles of water must have for all those of salt and alcohol, a uniform mixture cannot even be conceived. If but one particle of alcohol were less powerfully attracted than the surrounding particles, it would rise to the surface ; and in like manner, the particles of salt would, in consequence of their greater specific gravity, gradually occupy the bottom cf the vessel, were it not that a cause prevents them from rising or falling ; and this cause can be nothing but an attrac- tive force, which retains them in the place where they happen to be. The cause which effects a change in the place or in the properties of the ulti- mate particles or atoms of dissimilar substances, when these particles are in absolute contact, or at infinitely small distances from each other, as well as the cause which manifests itself as a resistance to such changes of place or properties, we call CHEMICAL ATTRACTION ;t and in this sense the mixture of two dissimilar liquids, the simple moistening of a solid body, the penetration and swelling of it oy a liquid, are effects in which chemical affinity or attraction has a decided share ; and although we are accustomed to limit the notion of affinity to such cases as exhibit a change perceptible to our senses, in the properties of the substances employed, as, for example, when sulphuric acid and lime, or sulphuric acid and mercury combine together, this limitation arises from the imperfect apprehension of the essence of a natural force. Every where, when two dissimilar bodies come in contact, chemical affinity is manifested. It is a universal property of matter, and by no means belongs to a peculiar class of atoms, or to a peculiar arrangement of these. But chemical combination is not, in all cases, the result of contact. Combination is only one of the effects of affinity, and occurs when the attrac- tion is stronger than all the obstacles which are opposed to its manifestation.^: When the forces or causes, which oppose chemical combination, heat, cohesive attraction, electric attraction or whatever they may be called, preponderate, then chemical combination does not take place ; and effects of another kind are manifested. Melted silver in a crucible, surrounded with red hot coals, in a place, therefore, where we should hardly anticipate the presence of free oxygen, absorbs as much (*) In these experiments membranes of all kinds may be used. With the thinner membranes, such as the bladder of the calf and the pig, the experiments are more rapidly completed than with the thicker, such as the gall-bladder and urinary bladder of the ox. The peritoneum of the ox and calf is preferable to all others. The tube c is tied with bladder under water. * Causes of the mixture of dissimilar liquids. H- Chemical affinity is the chief cause of mixture. } Affinity is everywhere active between bodies in contact. CHEMICAL AFFINITY IS UNIVERSALLY DIFFUSED. 19 as ten or twelve times its volume of that gas. Metallic platinum exhibits the same property in a far higher degree ; for from the atmospheric air, a gaseous mixture, in which oxygen forms only the fifth part, that metal (in the form of a black powder) condenses on its surface, at the ordinary temperature, an enormous quantity of oxygen gas (without any nitrogen,) and acquires thereby properties, which it does not otherwise possess.^) And when oxide of chromium, fragments of porce- lain, or asbestus, at high temperatures, effect the combination of two gases, oxygen and hydrogen, or oxygen and sulphurous acid, which gases do not combine at the same temperature, unless when in contact with these solid bodies, it is to the chemical attraction or affinity of these solid bodies that we must ascribe this effect. The solution of a salt in water is an effect of affinity, and yet no one property, either of the salt or of the solvent, is thereby altered, except only the cohesion of the saline particles. Sea salt, the crystals of which are usually anhydrous, takes up, at very low temperatures, 38 per cent, of water of crystallization ;* not because any new cause acts which increases its affinity for the particles of water (for cold is no cause, but the absence of a cause,) but because the higher temperature acted as an obstacle, opposing their chemical combination. The force of affinity is all the time present and undiminished. When we add alcohol to the solution of a salt in water, we observe, that now the salt separates from the liquid in the form of crystals, doubtless only because, by the addition of another chemical force, the amount of attraction between the particles of the salt and those of the water has been altered. The aqueous particles, which were combined with the saline particles, manifest an attraction for the particles of alcohol ; and as the latter have no affinity, or only a very feeble affinity, for those of the salt, the attraction of the saline particles for each other is strengthened. This attraction was present in equal force before the addition of the alcohol, but the resistance which opposed their union (the chemical attraction for them of the aqueous particles) was more powerful.! The alcohol was not the cause of the separation. The cause of the separation of the salt from the liquid, its crystallization, is at all times the force of cohesion ; and by the alcohol the cause which opposed its manifestation was removed. The affinity of potash for sulphuric acid is known, and sulphate of potash readily dissolves in water. If we add, to a saturated solution of that salt, an equal volume of aqua potassa3 of sp. g. 1.4, there is immediately formed a crystal- line precipitate of sulphate of potash, and the sulphuric acid is separated in this form from the water. In these cases the chemical effect (the separation) depends on the presence of a certain quantity of the liquid which is added (such as aqua potassae, alcohol, &c.,) but in many other cases there is required only a slight alteration in the quality of the solvent to effect separations of this kind. When hydrochloric acid is added to a solution of ferrocyanide of potassium, ferrocyanic acid is set free, and remains dissolved in the liquid. If now the vapor of boiling ether be passed through the mixture, there occurs, after a few moments, a complete separation. The whole of the ferrocyanic acid is deposited from the liquid in the form of white or bluish-white crystalline scales, which generally appear in such quantity as to render the whole mass semisolid. In proportion as the vapor of ether is dissolved by the water, the latter fluid loses entirely its solvent power (its affinity) for the ferrocyanic acid. The coagulation of albumen by ether depends on a similar cause. The capacity of solids to become moistened by liquids, and, in short, all (*) According to Doebereiner, platinum black condenses 252 times its volume of oxygen. Its effect in oxidizing alcohol, pyroxilic spirit, &c., is familiar to every chemist. W. G. * Crystallization of sea salt. I f Precipitation of salt from its solution by alcohol ; of sulphate of potash by caustic potash; of ferocyanic acid by ether ; of suspended mud by alum. 20 MOTION OF THE JUICES OF THE ANIMAL BODY. phenomena connected with chemical affinity, are affected, altered, increased, or de-stroyed by causes quite analogous. After heavy rains, the water of many rivers becomes turbid and opaque from the presence of a fine clay. These suspended particles of clay are so fine as to pass through the finest filters; and their adhesion to the water is so great, that such water does not clear after standing for weeks. The water of the Yellow River, in China, possesses, during the greater part of the year, this character ; and from the French missionaries, we know that alum is universally employed in Pekin to clear it. In fact, if a crystal of alum be held in such a water only for a few seconds, we observe the sediment separating in large thick fiocculent masses, the water becomes transparent, and hardly a trace of dissolved alum is to be detected by the most delicate re-agents. Chemistry is acquainted with a number of similar means for causing the separation from liquids of suspended precipitates- In these cases we see, that by an alteration of the quality of the water, produced by what we call mere mixture with a foreign body, its power of combining with others is destroyed or weakened. It is well known that the force with which, in a solution, the particles of the liquid and those of the dissolved body attract each other, is very unequal in different cases ;* and in this point of view the action of many solid bodies on saline solu- tions is very remarkable ; inasmuch as it is thereby demonstrated, that the mole- cular force, which determines the phenomena of cohesion, and the moistening of solid bodies by liquids appears to be identical with chemical affinity, since chemical compounds can be decomposed by means of it. Professor GRAHAM has shown that common charcoal, deprived by acids of all soluble ingredients, completely removes the metallic salts or oxides from solutions of salts of lead, tartar emetic, ammoniated oxide of copper, chloride of silver in ammonia, and oxide of zinc in ammonia ; while other solutions, such as that of sea salt, suffer no such change. A bleaching solution of hypochlorite of soda loses entirely its bleaching properties by agitation with charcoal ; and iodine can be removed by the same means from its solution in iodide of potassium. Every one is familiar with the action of finely- divided platinum, with that of silver on the deutoxide of hydrogen ; as well as with that of charcoal on dissolved organic matters, coloring matters, &c. ; and freshly-precipitated sulphuret of lead, sulphuret of copper, and hydrate of alumina, resemble the latter in their action. Many organic substances, such as woody fibre and others, act on dissolved matters, such as salts of alumina or of oxide of tin, just as charcoal does ; and we know that the application of mordants in dyeing, and dyeing itself depend on this very property. The adhesion of the solid coloring matter to the cloth which is died with it is the result of a chemical affinity so feeble, that we hardly venture to give the molecular force that name in this case. From a piece of woollen cloth dyed with indigo, the indigo is completely separated, by mere beating, continued for some time, with a wooden hammer, so that the wool is at last left white. The surface of the solid body exerts, as these facts prove, a very unequal attrac- tion on the molecules, which come in contact with it. Researches on capillary attraction have shown that, with one and the same liquid, water, for example, the substance of the solid body has no influence on the height to which the liquid rises on it. On slices of box-wood, clay-slate, or glass, the rise of the liquid above the surface of the water is the same exactly as in the case of a plate of brass. (HAGEN.) In the case of other liquids, the par- ticles of which are entirely homogeneous, the same law may be assumed in theory ; but with such liquids as contain foreign bodies in solution, a change in the capillary attraction must be produced by the presence of these bodies, because by them the cohesion of the liquid is altered ; and, perhaps, still more because the liquid ceases to be homogeneous, when the attracting wall has a stronger affinity for the particles of the dissolved body than for those of the solvent. From what has been stated, it appears, that the mixture of two liquids is the result of a chemical attraction ; for how otherwise could chemical compounds, * Action of solids on dissolved matters. LAWS OF THE MIXTURE OF DIFFERENT LIQUIDS. 21 such as the solution of a salt in water, be decomposed, or a chemical attraction be overcome, by its means ? Two liquids of different chemical properties, which are miscible together, and which, therefore, have a chemical attraction for each other, mix readily at all points where they come in contact.* By motion, shaking, &c., the number of points of contact within a given time is increased, and the formation of a uniform mixture is thus accelerated. If these liquids be of equal, or still better, of unequal, specific gravity, they may be, with the aid of some precaution, stratified one above the other. This is, in point of time, the most unfavorable case for the mixture, since proportionally small surfaces come in contact. But wherever they do come in contact, it is, after a very short time, impossible to detect any limit between them. In a cylindrical vessel containing solution of salt, the saline particles at the surface are attracted and sustained by aqueous particles, which exist at the sides of the saline particles and from the surface downwards. From the surface upwards, the attracting aqueous particles are absent. Now it is evident that when the surface is brought in contact with pure water, a new attraction is added to those previously existing, which acts in an opposite direction, namely, the attraction of the aqueous particles floating on the surface for the saline particles, and vice versa (the attraction of the saline particles to the aqueous particles in contact with them.) At the place where pure water and brine are in contact, there is thus formed a uniform mixture of the two, which upwards is in contact with pure water, down- wards with brine. Among these three strata, of which the upper contains no salt, the lower less water, a new division takes place ; the more strongly saline stratum loses salt, the pure water becomes saline, and in this way salt and water are at last uniformly distributed throughout the liquid. If we fill one limb of the tube (Fig. 7,) as far as a, with brine Fig. 7. colored blue, and the other limb with water, we find, in the course of a few days, the water colored blue, and the proportion of salt in both limbs equal.t It has been mentioned at p. 15, that, in a tube closed with bladder, filled with diluted solution of salt, and exposed to evaporation, the salt is not deposited in crystals on the outer sur- face of the bladder till the whole liquid in the tube has reached, in consequence of evaporation the maximum of saturation. The water evaporates from the exterior of the bladder, but no salt is de- posited, as long as a liquid exists within which can still dissolve salt ; and in this way the heavier saline particles are distributed to- wards the interior, and upwards through the whole liquid, or, what amounts to the same, the lighter aqueous particles, which can still dissolve salt, are distributed downwards towards the external surface of the bladder. This distribution of salt through water takes place in the same manner as the conversion of bar iron into steel.J Rods of malleable iron, as is well known, are kept ignited between strata of charcoal, whereby the surface of the iron in contact with the charcoal takes up carbon, and becomes a carburet of iron. The stratum of iron lying next under this surface, which has the same attraction for carbon, acquires carbon from the superficial stratum immediately in contact with it, and in its turn gives carbon to the stratum below itself. This process, if continued long enough, has no limit till all the strata of particles have acquired an equal proportion of carbon, that is, till they are all saturated with it. A piece of red-hot malleable iron, if kept a few moments in contact with pig iron (a carburet of iron) is found to be already converted into steel at the points of contact. The mixture of liquids depends on the same principle ; and we may suppose that th&L distri- * Laws of mixture 9f two liquids. t Experiment showing the uniform mixture of two liquids. JThe distribution of salt through water, resembles the conversion of iron into steel by cementation. 22 MOTION OF THE JUICES OF THE ANIMAL BODY. bution is mutual, because their particles may move in all directions, and that consequently saline particles move towards aqueous particles, as well as aqueous towards saline particles, in virtue of their mutual attraction. From a solution of sulphate of copper in ammonia, placed in a tall glass cylinder, there is gradually separated, if we pour a stratum of alcohol on the surface, and if we prevent -the formation of a coherent crust which impedes the contact of the liquids, the whole of the ammoniated sulphate of copper, while the deep blue solution becomes colorless, because by the distribution of the alcohol through the solution a mixture is formed, in which the salt is insoluble. TheVapidity of mixture of two liquids depends on the degree of their chemical affinity;* and the unequal mobility of the particles of one or the other liquid has a favorable or unfavorable influence on the result. When the one liquid is heavier than the other, and of tough, viscid consistence, a much longer time elapses before the ingredients of the tougher or heavier liquid reach the surface from the bottom of the vessel; and in this case the greater density and the less mobility of the particles are obstacles to the mixture. On the other hand, if the heavier OF more viscid liquid be placed above the lighter, the mixture takes place rapidly; at the points where both liquids are in contact is produced a mixture, which, being heavier, descends, whereby the heavier liquid above is continually brought in contact with new surfaces of liquid. The very same phenomenon is observed in solution. t A fragment of sugar, when covered with water at the bottom of a narroAv cylinder, dissolves very slowly, while, if suspended just below the surface, it rapidly disappears. In the former case there is produced round the sugar a thick syrupy viscid solution, which protects the undissolved part of the sugar for a long time from contact with the water ; in the latter there is formed at the surface a solution, which descends in striae, and gradually disappears, while by the change of place thus induced, new portions of water are constantly brought in contact with the undissolved sugar, and are thus enabled to exert their solvent powers. .If skin and membranes consist of a cohering system of very narrow tubes, it is obvious, that when two dissimilar, but miscible liquids are separated by such a tissae, the pores of the tissue will fill with each of the two liquids. In all situations, \*here the liquids came in contact in the substance of the membrane, a mixtnre takes place, and this mixture is extended equally towards both sides. If there be brine on one side of the bladder, and water on the other, there must be formed, in the middle, or at some point of the bladder, a diluted brine, which cr. tne side in contact with the water yields salt to that water, while on the opposite side the strong brine mixes with the diluted brine in the bladder. The substance of the bladder has no influence on this mixture, because it can produce no change of place on the part of the saline or aqueous particles, for this is the result of the chemical affinity acting between the particles of salt and those of water. J Now since the rapidity of the mixture of two liquids stands in a direct pro- .portion to the amount of surfaces coming into contact within a given time, and since the liquids, separated by a bladder, can only come in contact through its pores, while the number of points of contact is diminished by the presence of the non-porous parts of the bladder, it follows, that, exclusive of all other effects, the time required for mixture must be lengthened by the interposition of a bladder. In the absence of the bladder, the mixture would take place exactly as when it is present, except in regard to time. When the heavier brine is under, the water above the bladder, the two liquids mix more slowly than without the bladder. But since a bladder, inasmuch as a feeble hydrostatical pressure is not propagated through its pores, allows us to place a heavier liquid above a lighter, and to retain * Mixture is influenced by chemical affinity, by unequal mobility, and by unequal density in the liquids. t Effect of position on the solution of a solid. J Rapidity of mixture. CHANGE OF MIXTURE IN LIQUIDS. '23 it in that position; this circumstance has the effect of promoting mixture, the ultimate cause of which is, not the bladder, but the specific gravity of the liquid.* The bladder is a means of enabling the specific gravity to influence mixture. The foregoing remarks appear to me sufficiently to elucidate the share taken by the bladder in the mixture of two dissimilar liquids placed on opposite sides of'it. With respect to the change of volume in the two liquids which become mixed through the bladders, we must consider, that the moistening or absorbent power of a solid body, as well as the power of a liquid to moisten other bodies, is the result of a chemical action.! Liquids of different properties, or of different chemical characters, are attracted with unequal degrees of force by solid bodies, and exert towards them unequal degrees of attraction, and if we alter even in a system of capillary tubes, filled to a certain height with a liquid, the chemical nature of that liquid, we change thereby the height at which the liquid stands. In an animal tissue saturated with water, the water is prevented from flowing out by the mutual attraction, and by the capillary force, but if the attraction of the organic parietes for water be diminished by the addition of alcohol or of salt to the water, a part of it flows out. To this must be added, that the water absorbed by an animal texture when it enters the capillary tubes, exerts, in virtue of its attrac- tion for the tubes, a certain pressure, by which the vessels are swoln and enlarged. The particles of liquid in these tubes undergo a counter-pressure from the elastic parietes, by which pressure, when the attraction of the liquid particles for the solids is diminished by any new cause, the amount of expelled fluid is increased. The organic parietes of the tubes, saturated with water, are affected by alcohol just as a salt is when dissolved in water. On the addition of alcohol, or of another liquid, the water separates from the salt, or from the parietes, or the parietes separate from the water. If the animal tissue possessed as great an attraction for the newly-formed mixture as for the water alone, the volume of the liquid would not change. The mixture would take place, but no water would flow out. A bladder, saturated with water, when brought in contact with alcohol, shrinks together, a part of the water separates from the animal matter, but there always remains in the bladder a certain amount of water, corresponding to its attraction for the bladder and for the alcohol ; just as the solutions of many salts which have a strong attraction for water (such as a metaphosphate and acid phosphate of soda,) and are insoluble in alcohol, are separated by the addition of alcohol into two strata, of liquid, the heavier of which is a more concentrated solution of the salt in water, containing a little alcohol, while the other, the lighter, is an aqueous liquid con- taining much alcohol. The alcohol and the salt divide between them the water of the solution. When we add, to a mixture of equal parts of acetone and water, a certain quan- tity of dry fragments of chloride of calcium, the first fragments which are added deliquiesce and dissolve entirely in the mixture.:}: But if we go on adding the salt, a separation soon occurs, two strata of liquid are formed, of which the upper con- tains acetone and water, the other is an aqueous solutien of the chloride w r ith a little acetone. If we add still more of the chloride, water is abstracted from the acetone of the upper stratum, and when a proper quantity has been added, the acetone retains no trace of water. If we suppose, that of the two originally formed strata of liquid, one of them, namely that which sinks and contains chloride of calcium dissolved, is in contact with a current of dry air, the water of this solution will evaporate, the solution will thus become stronger, and in consequence of its increased concentration will be able to remove a new portion of water from the mixture of acetone and water above it ; and this will continue till the acetone is entirely deprived of water. If in the place of the chloride of calcium we put a bladder, and, in place of the acetone and water, diluted alcohol, we have the finest example of the unequal *In certain circumstances, the interposition of a membrane accelerates mixture, t Change of volume in liquids which mix through a membrane is the result of chemical affinity modifying capillary attraction. J Action of chloride of calcium on a mixture of acetone and water. 24 MOTION OF THE JUICES OF THE ANIMAL BODY. attraction which the animal tissue exerts on the two ingredients of the mixed liquid.* It is known from the experiments of SOEMMERING, that spirits of a certain strength, inclosed in a bladder, which is opposed to the air, lose by evaporation only water, and that at last anhydrous, or nearly anhydrous (absolute) alcohol is left in the bladder. When strong spirits of wine are used, the bladder remains dry externally ; when weaker spirits are employed, it becomes moist, and alcohol evaporates with the water. In virtue of the unequal affinity of the bladder for alcohol and for water, a complete separation is here effected. The water of the mixture is absorbed and evaporates from the outside of the bladder ; the alcohol remains in the bladder. As yet, we are acquainted with no substance which can replace the bladder in this operation ; and indeed, the affinity of the gelatinous tissues (membranes, &c.) for water must exceed that of all other animal tissues, since a rise of temperature, of a few degrees only, suffices to enable water to dissolve that tissue perfectly into a jelly. MEGNUS assumes, " that the particles of every solution, for example, of a salt in water, adhere more strongly to each other than do those of the solvent, for example, of water ; consequently, the solution would be less fluid, and pass with greater difficulty through very narrow openings, than water, if we take for granted that the parietes of the openings act alike towards both. It would follow from this, that, the more concentrated a solution, the less easily would it pass through the same openings."! " Let us now try," pursues MAGNUS, " with the aid of these assumptions, (which, as appears from the experiment Fig. 1, are perfectly accurate and demonstrable for many saline solutions, although there are, according to the researches of POISEULLE, a number of exceptions^) ) to explain the phenomena of ENDOSMOSIS." " Both the brine and the water will penetrate into the pores of the bladder, and brine will pass from the pores to the water, as well as water to the brine, in virtue of their mutual attraction, till a complete equilibrium is established. Further, since the force which attracts the water to the brine is exactly the same as that which attracts the brine to the water, as much water as brine would pass through the bladder, if both liquids could pass with equal facility through the pores. Since, however, this is not the case, unequal forces are required to urge the two liquids through the pores ; or with equal forces, unequal quantities of the two pass through in equal times. There is consequently added more of that which passes most easily, the water to the brine, than of the latter to the water, and the level of both liquids must change, if no other force oppose this change. "( 3 ) According to this theory, brine and water exist in the pores of the bladder in a state of motion, and the chemical affinity, which the particles of the brine have for the particles of the pure water, and conversely, which the particles of water have for those of salt, is considered as the cause of this motion. The unequal velocity, which makes more water flow in a given time to the brine than brine or salt to the pure water, is, according to MAGNUS, determined by the unequal resistance which the substance of the bladder opposes to the passage of the two liquids. Now, however narrow the tubes may be, in which molecules are set in motion by an external force, it may always be assumed, that that part of the molecules, which is immediately in Contact with the wall of the tube, either is not in motion, or possesses only a sirfall velocity, and the velocity of efflux must be a function of the cohesion, and at all events not dependent on the wall of the tube. If now the efflux of the water on one side of the bladder is produced by the attraction of the saline particles for the water, and the efflux of the brine on the oiher side is produced by the attraction of the aqueous particles for the saline *) Ann. de Ch. et de Phys. 3rd series, xxi. pp. 84 et seq. 2 ) PoggendorfFs Annales, x. p. 164. * Effect of evaporation through a bladder in concentrated alcohol, t Views of Magnus on Endosmosis. ATTRACTION OF THE MEMBRANE FOR LIQUIDS. 25 particles, it is impossible to explain how water and brine can move in the same tube with unequal velocity in opposite directions ; the two liquids being supposed to have a mutual attraction, that is, to be miscible. This attraction must act with- in the tube just as well without ; and we might, therefore, suppose, that when the two liquids have become mixed, the mixture could only move in one direction with a medium velocity. Assuming that a mixture is formed in the open orifices of the pores or tubes, or in any part of them, it is difficult to see, why saline particles should not pass from one side to the water, or aqueous particles to the saline ones in the bladder, since the mutual attraction must be regarded as equal on both sides. The chemical affinity of the two liquids does not explain the efflux. If we suppose, that in certain pores only brine, in others only pure water moves, the phenomenon ought not to occur when all the pores are filled with water or with brine, or when the tube is tied with a double, treble, or fourfold, bladder. But the properties of bladder are seen in the finest, as well as thickest membranes, and one, two, or three layers make no difference in the ultimate result. ( J ) The kind of influence which the nature of the partition, or its attraction for the liquids in contact with it, exerts on the phenomenon, is seen by comparing the action of an animal membrane with that of a thin sheet of caoutchouc.* In a tube, closed with bladder, which is filled with alcohol, and immersed in pure water, the volume of alcohol is increased ; more water passes to the alcohol than alcohol to the water.t If, without making any other change in the experiment, the tube be closed with a thin sheet of caoutchouc, the volume of the alcohol now diminishes while that of the water increases. Here, all the circumstances of the mixture of the two liquids have remained the same except the nature of the partition, which makes the difference in the result. When we fill with brine a tube, closed with bladder, (Fig. 8,) and place it in a vessel of water, so that the bladder and water only communicate by a single drop, the liquid in the tube increases in bulk, and rises in the tube, as if the bladder had been immersed in the water ; but the drop becomes gradually smaller, till after an hour or two, a complete separation takes place, and the drop tears itself away from the water.( 2 ) If the cause of the change of volume in this experiment were the unequal resistance which the bladder opposes to the passage of the two liquids with equal attraction (equal force) on both sides, the phenomenon just described would be inexplicable ; for a resistance can no doubt impede, but is not capable of producing motion. But we see, that the water in this experiment is raised to a higher level, and moreover, the tearing asunder of the drop can only be the effect of a powerful attraction, residing in the substance of the bladder. (') With respect to the theory, that, when a saline solution is mixed with pure the two liquids are separated by a membrane, particles of salt alone pass through the pores of the bladder to the water, and particles of water alone to the brine, the following experiments may throw some light on the question. For the sake of greater^accuracy, the results were determined by weighing. The apparatus, Fig. 3, was used. The tube contained 8'67 grammes of saturated brine, in which were 2'284 gram- mes of salt and 6*38 of water. After 24 hours it had gained 179 grammes in weight, and it now contained only 0-941 grammes of salt. It had therefore lost 1-343 grammes )t salt, and gamed 3'13 of water. According to the above theory, 1 atom of salt and [ 2 ] If we pour into a tube, J of an inch wide, and closed with bladder, as much mercury as covers the surface of the bladder, then fill it with brine, and place it in pure water the volume of the liquid in the tube increases exactly as if the mercury were not there. J * The nature of the membrane has an important influence. r Experiment with bladder, and with caoutchouc. 4 26 MOTION OF THE JUICES OF THE ANIMAL BODY. If the moistening of solid bodies by liquids be the effect of a chemical attraction the force of which is different in dissimilar liquids, it follows that, when a porous body is saturated with a liquid, and brought in contact with a second liquid, which has a stronger attraction for its substance than the first has, then the liquid must be displaced from the pores by the second, even in the absense of hydrostatic pressure, and this, whether the two liquids be miscible or not.* We may suppose that the attraction of the second liquid, of more powerful affi- nity, which displaces the other, is equal to the pressure of the column of mercury required to force the latter through the porous substance. If we tie over one end of a cylindrical tube with a very thin membrane, saturated with concentrated brine by steeping 24 hours, and if we dry the outer surface of the membrane carefully with bibulous paper, and now pour a few drops of pure water into the tube so as just to cover the inner surface of the membrane, the outer surface is seen in a few moments to be covered with minute drops of brine ; that is, brine flows out of the pores of the bladder. A thick ox-bladder, saturated with oil, exhibits the same phenomenon in contact with water. The oil is expelled from the pores of the bladder by the water, which occupies its place. When the bladder is brought in contact with pure water, it takes up a certain quantity of that liquid. If its pores are previously filled with brine, and if we cover one side of it with pure water, the water mixes with the brine in the pores of the bladder ; and on the side next the water there is formed a diluted brine, which, being in contact with a stratum of pure water, mixes with it, and in this way the successive strata of water receive, from the bladder outwards, a certain quantity of salt. In the interior of the bladder, there are forrned in like manner, towards the outer surface, mixtures of unequal saline strength. If we suppose the bladder to consist of several strata, all these strata receive, from the surface in contact with the water, a certain quantity of water ; the outer stratum, in contact with the air, receives least, and is the most highly charged with salt. The cause of mixture is the cheminal affinity of the salt for the newly-added particles of water ; this affinity is equal on both sides, but the attraction of the sub- stance of the bladder is stronger for the more aqueous or less saline liquid, than for the more concentrated. In consequence of this difference in the attraction of the liquids for the substance of the bladder, a part of the mixture is displaced from the bladder; the less saline liquid takes the place of the more saline; a part of the latter is expelled, and, with it, a part of that water which has been added to the outer stratum by mixture. Brine and water flow out in the direction of least resist- ance. The efflux towards the side on which the pure water was poured is prevented by the more watery liquid for the substance of the bladder. If we remove from the outer surface of the bladder the displaced saline liquid (which has been mixed with some water,) and put stronger brine in its place, and if on the opposite side we remove the very diluted solution, replacing it by a still more diluted one, the same process is repeated. There arises a permanent differ- ence, and a state of mixture and efflux continues till the liquids on the opposite surfaces of the bladder have the same, or very nearly the same, composition. If we suppose, that the two liquids moisten the bladder unequally, it follows, * One liquid expels another from a membrane. 15 atoms of water must have moved past each other; but this is impossible, siace 1 atom of salt requires 18 atoms of water for solution, (10 parts of salt to 27 of water.) The weight of the pure water in the outer vessel was 19*26 grammes ; consequently, the weight of the brine was to that of the pure water as 1 : .2*22. In another experi- ment, in which the weight of the brine in the tube was to that of the water outside, as 1 : 7*98 ; the tube gained 0'822 grammes in weight ; the liquid in the tube contained at first 0^)47 grammes of salt ; and 24 hours after, O148 grammes: hence, 1'621 grammes of water had entered, while O799 grammes of salt had passed out. For 1 atom of salt, which passed from the tube with brine to the vessel with water, there .passed from the latter to the former rather more than 13 atoms of water; (for 58'6 parts, or 1 atom of aalt, 118 parts of water.) ATTRACTION OF LIQUIDS FOR MEMBRANES. Fig. 9. that in addition to the chemical attraction which the dissimilar particles of the } liquids have for each other, a new cause, namely, the strong attraction of one of I them for the substance of the partition, is introduced, which accelerates* their motion I or passage, and must have this effect, that one of them flows out in larger quantity, in the same time, than the other. The experiments (Fig. 3) elucidate this process, and show besides, that the exchange of the two liquids on both sides of the bladder is essentially determined Lby their unequal specific gravities.* As long as the difference in their composition (which may here be measured by the specific gravity) is very great, the change of pvolume (increase of one and decrease of the other) takes place rapidly ; but at [last, when this difference becomes very small, the liquids mix without further i visible change of volume, obviously, because the attraction of the bladder to ihe I mixtures on the opposite sides does not perceptibly differ, although the specific gravities are still somewhat unequal. In the ultimate result, the action of dissimilar liquids on the substance of animal tissues, in consequence of which their mixture is attended with a change of volume, appears to be equivalent to a mechanical pressure, which is stronger from one side than from the other.t If the tube (Fig. 9,) which is closed with bladder at its wide opening, be filled with brine to the mark a, if so much mercury be then poured into the narrow vertical part as by its pressure to cause brine to begin to flow out in fine drops from the pores of the bladder, and if now, after removing so much of the mercury that the efflux is no longer visible, we place the apparatus in a vessel with pure water, colored blue, as in the figure, the mercury does not change its level ; and when, after one or two hours, we carefully remove the tube from the water, we find that in the upper part of the wide end of the tube, which con- tained colorless brine, a dark blue stratum has been formed, which floats on a colorless liquid. After a longer time, the blue color spreads gradually downwards, till at last the brine acquires a uniform blue tint. It will readily be perceived, that the two liquids here mix, as if no pressure had been applied to the brine, for a mechanical pressure exerts no influence on the mixture ; but, in consequence of the pressure, the mixture takes place without change of volume. The mechanical pres- sure which the water, in virtue of its stronger affinity for the bladder, exerts on the brine in the pores of the bladder, is held in equilibrium by the column of mercury, and the result is that exactly as much brine flows out as water flows in. Let us suppose the column of mercury to be removed, and the rise of the brine in the narrow tube is explained at once. If we close a short tube, filled with alcohol or brine, with bladder at both ends (an arrangement which may represent a cell,) and suspend it in a vessel of pure water, both surfaces of the bladder become convex outwards ; they swell, but without bursting. As soon as the pressure, gradually increasing by the influx of water into the interior of the tube, is sufficient to keep in equilibrium the affinity of the water for the bladder, and consequently its further influx, the exchange goes on, for the future, without change of volume. Most porous bodies exhibit the phenomena described in the preceding pages, if their pores are so minute that a feeble hydrostatic pressure is not propagated * Mixture is essentially determined by the unequal density of the liquids. o osrte "sides 1 n anima * tissues equivalent to a mechanical pressure, unequal on t Experiment to snow tnat an external pressure prevents change of volume. $ Additional experiment. 28 MOTION OF THE JUICES OF THE ANIMAL BODY. through them.* These phenomena may be produced with clay cells ( J ) (such as are used for galvanic apparatus ;) with the lining membrane of the pods of peas and beans ; with the fine inner bark of trees ; with the skin of grapes, of potatoes, of apples ; with the inner membrane of the capsules of bladder senna, &c. ; but animal tissues surpass all others in efficacy. Besides their unequal affinity, they have an unequal absorbent power for dissimilar liquids, by which their action in causing change of volume during mixture is strengthened. When a tube, closed with bladder, and filled with water, is immersed in alcohol or brine, there is produced at all points, where the brine or the alcohol comes in contact wkh bladder saturated with water, a change in the properties of the bladder.! When, in the open pores, the alcohol or brine mixes with the water already there,- the absorbent power of the bladder for the water is diminished ; a smaller volume of the mixture is retained than of pure water ; that is to say, water flows out in the direction of the alcohol or brine. This efflux is accompanied by a change in the volume of the substance of the bladder, for that side of it which is towards the alcohol or the brine contracts or shrinks. The opposite surfaces of an animal membrane, in contact with dissimilar liquids, for which they have unequal absorbent power, are in an unequal state of contraction. This condition is permauent, as long as the liquids do not change in their proper- ties ; but it ceases, in consequence of mixture, and is again restored, when, by means of the change of place in both the liquids which are in contact with the opposite sur- faces of the bladder, the original or any other permanent inequality or difference of properties is produced. In all cases where a permanent change in the volume of two liquids, separated by a membrane, is observed during their mixture, it is always accompanied by a permanent difference in the nature or properties of the two liquids ; and from this it follows, that the molecules of the animal membrane must be, during the mixture, in an alternate state of contraction and swelling, or dilatation ; that is, in a continual motion.^ From what has been stated, it appears that the change of volume of two miscible liquids, separated by a membrane, is determined by the unequal capacity of being moistened, or the unequal attraction of the membrane for these liquids. The une- qual absorbent power of the membrane for these liquids depends on the dissimilar nature of the liquids or of the substances dissolved in them. An unequal proportion (') I consider it of sufficient importance to state here that porous clay also takes up unequal volumes of brine and water. In special experiments made on this subject, cells of clay (moderately ignited porcelain biscuit) were laid for 24 hours in pure water, then carefully dried externally with bibulous paper, and the increase in weight, that is, the weight of the absorbed water, carefully determined. $ The clay was then carefully dried, laid for 24 hours in brine, and the weight of the absorbed brine determined in like manner. In a second series of experiments, the clay cells were steeped in water and brine, and placed in the receiver of the air-pump, under a pressure of 8 lines of mercury ($ of an inch) for 24 hours. Under the ordinary pressure, and in air the cells absorbed Weight. Volume. Water. Brine. Water. Brine 100 parts of clay cell I. 15'4 14'6 15-4 12-2 II. 11-8 11-6 11-8 9-7 In vacuo the cells of clay absorbed Weight. Volume. Water. Brine. Water. Brine. 100 parts of clay cell absorbed I. 16-5 16-8 16-5 14-0 II. 13-8 13-8 13-8 11-5 * Porous bodies in general exhibit similar phenomena. t Bladder shrinks in contact with brine or alcohol. i Change of volume in two liquids, separated by membrane, is accompanied by continual motion in the particles of the membrane ; and depends on the unequal attraction of the membrane for the liquids. $ Amount of liquids absorbed by porous baked clay. EXAMPLES OF CHANGE OF VOLUME IN LIQUIDS. 29 of the same dissolved matters (unequal concentration,) acts in many cases, just as if the liquids contained dissimilar substances. Although the experiments hitherto instituted, and the results obtained by FISCHER (who first observed these phenomena,) MANGUS, DUTROCHET, and others, admit of no comparison, since the apparatus used by them showed only relative change of volume, yet a knowledge of some of these results is, nevertheless, of importance. When the two liquids are, diluted sulphuric acid (of sp. g. 1-093) and water, the acid, at 50 F., increases in volume ; but if the acid have the specific gravity 1-054, the volume of the water increases,* Diluted tartaric acid (11 parts of the crystalized acid and 89 of water) and water mix through a bladder without change of volume ; with more than 11 per cent, of acid, the volume of the acid increases ; with less that of the water. Solutions of animal gelatine, gum, sugar, and albumen increase in volume when separated by a bladder from water ; and the increase of volume in these different solutions, although of the same specific gravity, is very different indeed. When the specific gravity is 1-07, the increase in volume of the solution of gelatine amounts to 3, that of solution of gum to 5, of sugar 11, of albumen 12. When a solution of sugar (1 part of sugar to 16 of water) is separated by a bladder from water, it increases in volume ; but if we add 1 part of oxalic acid to the sugar, the water, on the contrary, increases in volume. If the amount of sugar in the solution be doubled, the liquids mix without change of volume. A solution of sugar, separa- ted by bladder from one of oxalic acid, rises, in the same time, 3 times higher than when separated from water. (DUTROCHET.) From these experiments we obtain, as a universal result (which, however, requires confirmation,) that an animal membrane possesses a less power of absorption for solution of albumen than for all other organic substances :t and that a small amount of mineral or organic acids increases the power transudation of water as well as of the solutions of many organic substances.^ 1 ) The rapidity of mixture of two liquids, separated by a membane, depends on the thicknes of the membrane, and stands in direct proportion to the velocity with which the mixture formed in the pores and on both surfaces of the bladder changes i its place, and the original difference in the quality of the two liquids is renewed. || If we suppose a tube, formed of a membrane (an intestine, for example,) and filled with water, and if we assume that a current of saline solution flows round this tube, in consequence of a mechanical force, the increase of volume of the brine ' (the passage into it of a certain amount of water) will be effected in a far shorter time than if the brine were not in motion. The velocity of transference will diminish with the amount of difference in properties between the two liquids (the different amount or percentage of salt;)^[ it will be greatest at first, and diminish as the dilution of the brine increases, in proportion, that is to say, as water is transferred from the contents of the tube to the liquid without. The greatest effect, therefore, must occur and be permanent, when the water transferred to the brine is continually again removed from it, that is, when the con- centration of the brine is kept uniform.** To this end, if we suppose the membrane (*)In order not to be misled in such experiments, we must avoid the employment of all those liquids which alter the membrane in its chemical properties. Such are, for exam- ple, acids of a certain concentration, nitrate of silver, salts of lead, chloride of gold, chlo- ride of tin, chromic acid, bichromate of potash, taunic acid, &c. Even in water, the properties of membranes generally undergo a change after some days, they then propa- gate a far -sveaker hydrostatic pressure through their pores, and are no longer fit for such experiments. * Examples of change of volume ; in acids, and neutral organic substances, according to DUTROCHET t Membranes have a feeble power of absorbing solution of albumen. I Effect of adding acids. $ Causes which influence rapidity of mixture. || Motion of one of the liquids. If Difference in properties of the two liquids. ** Effect of the continual removal of the transferred liquid analagous to suction. SO MOTION OF THE JUICES OF THE ANIMAL BODY. to be difficultly permeable for one liquid, while the other is easily taken up into its pores, and if we reflect, that this second liquid, on entering into the pores of the bladder, in virtue of the attraction of their walls for it, acquires a certain velocity which permits it to pass beyond the extremitiss of the canal or the pores, so as to entirely fill the pores, and to come in direct contact with the liquid on the outside of the pores, it follows, that, when this second liquid moves past the poses with a certain velocity, the absorbed liquid must follow it during the mixture, and there must take place a rapid transference of the second liquid to the first, a true suction as if by a pump. The animal body is an example of an apparatus of this kind in the most perfect form.* The blood vessels contain a liquid, for which their walls are, in the normal state, far less permeable than for all the other fluids of the body. The blood moves in them with a certain velocity, and is kept at all times in a nearly uniform state of concentration by a special apparatus, namely, the urinary organs. The whole intestinal canal is surrounded with this system of blood vessels, and all the animal fluids, in so far as they are capable of being taken up by the parietes of the intestinal canal, and of the blood vessels situated around it, are rapidly mixed with the blood.t The volume of the blood increases, if no compensation is effected by means of the kidneys: and the intestine is emptied of the liquids contained in it. The intestinal glands, through which this transference is effected, and each of which represents a similar apparatus of suction, contain, within them, two systems of canals, blood vessels and lacteals; the blood vessels are placed next to the external absorbent surface, the lacteals chiefly occupy the central part of the gland. The liquids circulating in these two systems have very unequal velocities, and as the blood moves much faster in the blood vessels, we perceive how it happens, that the fluids of the intestine are chiefly (in quantity and in velocity) taken up into the circulation. The difference in the absorbent power of the parietes of the intestinal canal for liquids which contain unequal amounts of dissolved matters, is easily observed in the effects produced on the organism by water and saline solutions.^ If we take while fasting, every ten minutes, a glass of ordinary spring water, the saline contents of which are much less than those of the blood, there occurs, after the second glass (each glass containing 4 ounces,) an evacuation of colored urine, the weight^of which is very nearly equal to that of the first glass ; and after taking, in this way, 20 such glasses of water, we have had 19 evacuations of urine, the last of which is colorless, and contains hardly more saline matter than the spring water. If we make the same experiment with a water, containing as much saline matter as the blood (| to 1 per cent, of sea salt,) there is no unusual discharge of urine, and it is difficult to drink more than three glasses of such water. A sense of repletion, pressure, and weight of the stomach point out, that water as strongly charged with saline matter as the blood requires a longer time for its absorption into the blood vessels. Finally, if we drink a solution containing rather more salt than the blood, a more or less decided catharsis ensues. The action of solution of salt is of three kinds, according to the proportion of salt. Spring water is taken up into the blood vessels with great rapidity ; while these vessels exhibit a very small power of absorption for water containing the same proportion of salt as the blood does ; and a still more strongly saline solu- tion passes out of the body not through the kidneys, but through the intestinal canal. Saline solutions and water, given in the form of enemata, exhibit similar phenomena in the rectum. || Pure water is very rapidly absorbed, and excreted * This occurs in the animal body. t Absorption of the liquids of the intestines into the blood. j Effects produced by drinking water and saline solutions. $ Solution containing more salt than the blood. H Enemata of water and saline solutions. INFLUENCE OP THE MEMBRANES ON THE SECRETIONS. 31 through the urinary passages. If we add to the water colored or odorous matters, these appear, more or less changed in the urine. When a small quantity of ferrocyanide of potassium is added, its presence in the urine is very soon detected by chloride of iron, which forms with it Prussian blue. Of concentrated solutions far less is absorbed in the same time, than of diluted ; in most cases they mix with solid matters collected in the rectum, and are expelled in the form of a watery dejection. All salts do not act alike in this respect. In equal doses, the purgative action of Glauber salt and Epsom salt is far stronger than that of sea salt ; and their power of being absorbed by animal membranes appears to be in the inverse ratio of this effect. It is hardly necessary, particularly to point out that an explanation of the action of purgatives in general cannot be included in the above-described action of saline solutions on the organism. The example which has been given is intended to illustrate a physical property common to a large number of salts, and apparently of the nature of the acid or base of the salt ; for chloride of calcium, chloride of magnesium, bitartrate of potash, tartrate of potash and soda, phosphate of soda, and certain doses of tartar emetic, show the same action as sea salt, Glauber salt, and Epsom salt, although the bases and acids in these different salts are not the same. Solutions of cane sugar, grape sugar, sugar of milk, and gum, exhibit, when separated from water by an animal membrane, phenomena similar to those exhibited by the above-named solutions of mineral salts, without causing in the living body a purgative action, when of equal concentration. The cause of this difference may be that the mineral salts, in their passage through the intestinal canal, and through the blood, are not essentially altered in their composition, while these organic substances, in contact with the parietes of the stomach, and under the influence of the gastric juice, suffer a very rapid change, by which the action which they have out of the body is arrested. Since the chemical nature and the mechanical character of mebranes and skins exert the greatest influence on the distribution of the fluids in the animal body, the relations of each membrane presenting any peculiarity of structure, or of the different glands and systems of vessels, deserve to be investigated by careful experiment ;* and it might very likely be found that in the secretion of the milk, the bile, the urine, the sweat, &c., the membranes and cell-walls play a far more important part than we are inclined to ascribe to them ; that besides their physical properties, they possess certain chemical properties, by which they are enabled to produce decompositions and combinations, true analyses ; and if this were ascer- tained, the influence of chemical agents, of remedies, and of poisons on those properties, would be at once explained. The phonomena described in the preceding pages. are observed, not in the gelatinous tissues alone, but also, apparently, in many other structures of the animal body, which cannot be reckoned as belonging to that class.t If we tie moist paper over the open end of a cylindrical tube, and, after pouring in above the paper white of egg to the height of a few lines, place that end of the tube in boiling water, the albumen is coagulated, and when the paper is removed, we have a tube closed with an accurately fitting plug of coagulated albumen, which allows neither water nor brine to run through.^ If the tube be now filled to one-half with brine, and immersed in pure water, as in Fig. 4, the brine is seen gradually to rjse ; and in three or four days it increases by from ? to 5 of its volume, exactly as if the tube had been closed with a very thick membrane Influence of the cutaneous evaporation on the motion of the fluids of the animal body. When a tube about 30 inches long, bent in the form of a knee, and widened at one end, is tied over at that end with a piece of moist ox-bladder, the bladder now * Influence of membranes on secretions. t These phenomena not confined to the gelatinous tissues. + Coagulated albumen acts like a thick membrane. MOTION OF THE JUICES OF THE ANIMAL BODY. thoroughly dried, and the tube filled with mercury and inverted, so that the open, narrow end stands in a cup of mercury, the mercury in the tube falls to about 27 inches (Hessian,) and remains, if the bladder have no flow, at that height, rising and falling as the mercury does in a barometer. No air passes through the dry bladder into the Torricellian vacuum thus pro- duced. When, by proper manipulation, we have allowed to pass out as much as can be removed of the air still contained in the tube, we have, in this arrangement, a barometer, containing no more air than would be found in one made with a similar tube hermetically sealed at the wide end, provided the mercury in the latter had not been boiled in the tube to expel the last traces of air. By the desiccation' of the bladder, its pores, which allowed a passage to water, brine, oil, or even mercury, have obviously been closed by the adhesion of the successive layers of membrane, which perhaps cross each other, so that the bladder is not more permeable for the particles of air than a slice of horn of the same thickness. Fig 10 ^ we mtro(mce wate r into the tube in the posi- ' m tion, Fig. 10, to the line marked , and, after fill- Fig. 11. ing the narrow part of the tube with mercury, invert it in a vessel of mercury, Fig. 11, we observe a number of minute bubbles of air passing through the moist bladder into the tube. The mercury falls to a certain point, which is higher or lower according to the thickness of the bladder; it stands at a lower level with a thin membrane than with a thick one. When a single layer of ox-bladder is used, it falls to 12 inches (above the level of the mercury in the vessel ;) with a double layer it stands at from 22 to 24 inches. -If we take care to allow the water standing above the mercury to enter the Wide part of the tube, so that the bladder is kept at all times covered with water, the mercury remains stationary at the same level. If, for example, it stood at 12 inches, it remains there, although the quantity of water is constantly diminishing by evaporation from the bladder ; and it maintains its level, even after all the water has disappeared. The height of the mercury in the narrow tube is an exact measure of the pres- sure acting on the surface of the bladder. The pressure in the inside of the tube is less than the existing pressure of the atmosphere outside by the height of that column of mercury. This difference of level between the mercury in the vessel and that in the tube is the limit of the pressure, under which air passes into the water through the pores of the bladder ; or under which the molecules of water in the pores are displaced by the molecules of air. If we fill the tube entirely with water, and place the narrow end in mercury, while the wide end, closed with bladder, is exposed to the air, the mercury rises in the narrow limb, and at last reaches a point, identical with that to which it fell in the preceding experiment. For each specimen of bladder, according to its thickness, the level to which the mercury reaches is of course different. When the diameter of the wide part of the tube, which is closed with bladder, is 12 millimetres, and that of the narrow tube 1 millimetre, the mercury rises, with ox-bladder, according to the temperature and the hygrometric condition of the air, to from 22 to 65 millimetres in one hour. The cause of the rise of the mercury in this experiment hardly requires a special explanation. The bladder is penetrated with water, covered on one side with water, and on the other in contact with a space (the air) not saturated with aqueous vapour The water contained in the pores of the side of the bladder turned towards the air ATMOSPHERIC PRESSURE ON THE LEVEL OF THE MERCURY. 33 evaporates ; the space which it had occupied in the pores is filled with successive portions of water from within, in virtue of the attraction of the substance of the pores for water. The volume of the water in the tube diminishes, and thus a vacuum arises, in which the mercury is forced to rise by the atmospheric pressure. The space formerly occupied by the water which has evaporated is now filled with mercury. When the mercury has reached a permanent level, the external pressure, which acts on the water in the pores of the bladder (and which tends to displace the parti- cles of water) is obviously equal, before air enters, to the attraction which the substance of the bladder has for the particles of water, and these last to each other. Were the attraction less, air would enter, and the particles of water could not main- tain their position. The rise of tke mercury, or its motion towards the surface of the bladder, that is, towards the point where evaporation is going on, is the result of a difference of atmospheric pressure, determined by the evaporation of the water, or of the liquid which penetrates through the bladder, and by the absorbent power of the bladder for that liquid. One chief condition of the efficiency of a bladder, in regard to the rise of a coulmn of liquid, is, that it is kept constantly in contact with the liquid, for without this contact the absorbent power cannot manifest itself. By the evaporation a continual efflux of water, in the form of vapour, towards the side on which the air lies, is produced ; and by the capillary action of the blad- der on the other side, water is absorbed and retained with a force which counter- poises 12 or more inches of mercury, according to the thickness of the bladder. Now, since the rise of the mercury is an effect of the atmospheric pressure, it is plain, that the height to which the mercury rises, must depend to a certain degree on the state of the barometer.* In a tube filled with water, and closed with bladder, the absorbent force of which is equal to the pressure of a column of 12 inches of mercury, the mercury rises by evaporation to the height of 12 inches, as long as a column of 12 inches of mercury can be sustained by the external atmospheric pressure. If this external pressure fall below that limit, the mercury in the evaporation tube falls to the same extent, and if there be water above the mercury, this water separates from the bladder. This property of bladder, therefore, would appear unaltered at an elevation at which the barometer should stand at 12 inches ; at a still greater elevation, on the contrary, the liquid would separate from the bladder. The external pressure has no influence on the amount of the water evaporating in the pores of the bladder ; that amount depends on the hygrometric state of the surrounding air, and on the temperature.! In a rarified air, (provided it can take up moisture,) evaporation goes on more rapidly than in a denser air ; and hence it is clear, that at certain elevations, the effect of the bladder on the level of the liquid is more quickly produced than at the level of the sea. The amount of water which evaporates is directly propor- tional to the surrounding space, and to the temperature and corresponding tension of the liquid. When the tube, Fig. 10, is filled with water to 6, then entirely filled with mercury and inverted in mercury, the Fig. 10. mercury, as we have seen, assumes a fixed level. If we now keep the upper or wide end of the tube, which is closed with bladder, immersed in a vessel of water, Fig. 12, we shall find, after a short time, that the mercury sinks in the narrow tube. If its level has been 12 inches above that of the mer- cury in the vessel, it sinks when the bladder is put into water, 3 or 4 inches for example, and remains stationary at 8 or 9 inches, without sinking further for the next 12 hours. * Influence of the state of the barometer t The pressure of the air does not affect the amount of evaporation. 5 34 MOTION OP THE JUICES OF THE ANIMAL BODY. The sinking of the mercury is caused by water being forced through the bladder into the tube, in virtue of the existence of an external pressure greater than the pressure on the inside of the tube. To displace the aqueous particles in the pores of the bladder by other aque- ous particles, requires obviously a much smaller pressure than is necessary to dis- place them by particles of air.* In the one case, where both surfaces of the bladder are in contact with the liquid, the attractive force (that of the bladder for the water and of the water for the bladder) is equal on both sides ; but not so in the other case, where one side of the bladder is in contact with air. If the bladder had the same absorbent power for the particles of air as for those of water, the particles of air and water would pass through the bladder under the same pres- sure ; the experiment shows, that the absorbent power and permeability of the bladder for air is far less than for water. Hence, it comes to pass, that when, with a given portion of bladder, in the apparatus Fig. 11, mercury is raised by evapora- tion to a heighth of 12 inches, less than 12 inches of mercury are required, in the apparatus, Fig. 1, to cause water to pass through the bladder. t When the tube, (Fig. 13,) is filled with water, closed with blad- der at both ends, and exposed to evaporation, the bladders in a short time become concave, that is, they are pressed inwards. As the evaporation of the water through the moist surfaces of the bladder proceeds, there is formed in the upper part of the tube a vacuum, which is filled with aqueous vapor, and which continues to increase. The place of the water which evaporates is, as in the experiments previously described, gradually occupied by air, which enters the tube through the bladder. It is evident, that when air enters the tube, (Fig. 13,) the pressure on the surface of the bladder is equal to the absorbent force of that bladder for the water. In the apparatus, Fig. 11, with the same bladder, the mercury might have been raised, in consequence of the evaporation, to a height of 4, 6, 12, or more inches, according to the thickness of the membrane. -. When the longer limb of the bent tube, after it has been >' filled with water, and closed at both ends with bladder, is placed in a vessel containing brine, and exposed to evaporate in the air, as in Fig. 14, it is plain, that when the atmospheric pressure, increasing in consequence of the evaporation of the water on both the surfaces of the bladder, reaches the point at which the brine flows through the pores of the bladder, then the place of the water which evaporates is occupied by brine. In fact, when the brine is colored blue, we observe, after a few hours, that a blue stratum forms within the tube, which constantly increases, till at last the vessel of brine is emptied, and the tube is entirely filled with brine. If the longer limb be immersed in bile instead of brine, the tube fills with bile, and if we employ, for closing one end, a membrane rather thinner than we use for the other, from which the evaporation takes place, and then place the end with the thinner membrane in oil (oil of marrow,) the tube gradually fills with oil. In all these cases, no air enters the tube, which continues full of liquid, as it was at first. J If we connect the evaporation tube by collars of caoutchouc with short bits of tube (Fig. 15,) full of water, and tied with bladder at both ends; and if we immerse the last bit of tube in brine, urine, oil, &c., all these cells, and at last the * Water passes through moist bladder more easily than air does. f Experiments with a tube closed at both ends with bladder : with one end in brine, the tube being filled with water, with one end in bile, and in oil. J Effect of a series of short tubes, closed at both ends with bladder. IMPORTANCE OF THE CUTANEOUS TRANSPIRATION. 35 Fig. 15. evaporation tube itself, become gradually filled with brine, urine, oil, &c. P\ * The most general expression for these experiments and results ' tnat a ^ liquids which are in connection with a mem- j brane from the surface of which evaporation can take place, must I acquire motion towards that membrane. The amount of this motion is directly proportional to the rapidity of evaporation, and consequently to the temperature and hygrometric state of the atmosphere. That the skin of animals, and the cutaneous transpiration, as well as the evaporation from the internal surface of the lungs, exert an important influence on the vital processes, and thereby on the state of health, has been admitted by physicians ever since medicine has existed ; but no one has hitherto, ascertained precisely in what way this happens.! From what has gone before, it can hardly be doubted, that one of the most important functions of the skin consists in the share which it takes in the motion and distribution of the fluids of the body 4 The surface of the body of a number of animals consists of a covering or skin permeable for liquids, from which, when, as in the case of the lung, it is in con- tact with the atmosphere, an evaporation of water, according to the hygrometric state and temperature of the air, constantly goes on. If we now keep in mind, that every part of the body has to sustain the pressure of the atmosphere, and that the gaseous fluids and liquids contained in the body oppose to this pressure a perfectly equal resistance, it is clear that, by the evapora- tion of the skin and lungs, and in consequence of the absorbent power of the skin for the liquid in contact with it, a difference in the pressure below the surface of the evaporating skin occurs. The external pressure increases, and in an equal degree the pressure from within towards the skin. If now the structure of the cutaneous surface does not permit a diminution of its volume, a compression (in consequence of the loss of liquid by evaporation,) it is obvious that an equalization of this difference in pressure can only take place from within outwards ; first from within, and especially from those parts which are in closest contact with the atmosphere, and which offer the least resistance to the action of the external pressure. || Hence it follows, that the fluids of the body, in consequence of the cutaneous and pulmonary transpiration, acquire a motion towards the skin and lungs, which must be accelerated by the circulation of the blood. By this evaporation, the laws of the mixture of dissimilar liquids, separated by a membrane, must be essentially modified.^ The passage of the food dissolved in the digestive canal, and of the lymph into the blood vessels, the expulsion of the nutritive fluid out of the minuter blood vessels, the uniform distribution of these fluids in the body, the absorbent power of the membranes and skins, which, under the actual pressure are permeable for the liquids in contact with them, are under the influence of the difference in the atmospherical pressure, which is caused by the evaporation of the fluids of the skin and lungs. The juices and fluids of the body distribute themselves, according to the thick- ness of the walls of the vessels, and their permeability for these fluids, uniformly through the whole body; and the influence which a residence in dry or in moist air, at great elevations or at the level of the sea, may exert on the health, in so far as the evaporation may thus be accelerated or retarded, requires no special explana- tion ; while on the other hand the suppression of the cutaneous transpiration must * Liquids move towards the membrane from which evaporation takes place. h Influence of the skin and cutaneous transpiration on health. \ The cutaneous evaporation has an important share in causing the motion of the animal fluids. * Evaporation is constantly going on from the skin and lungs. II This evaporation must produce unequal pressure, by which the fluids acquire a motion to- wards the skin and lungs. \ The change of pressure influences the mixture of the fluids. 36 MOTION OF THE JUICES OF THE ANIMAL BODY. be followed by a disturbance of this motion, in consequence of which the normal process is changed where this occurs. The pressure, which, in consequence of the evaporation, urges the fluids within the body to move towards the skin, is, as may readily be understood, equal to the difference of pressure acting on the surface of the skin.* From the experiment, Fig. 13, it is plain, that when one of the two surfaces of bladder at the ends of the tube Fig. 12, is exposed to atmospheric evapopation, while the other end is moistened with water, brine, or oil, these liquids are rapidly absorbed by the membrane, that is, are forced in by the external atmospheric pressure, and it is not less obvious, that the same thing takes place with the liquid with which one of the two evaporating surfaces has been moistened in the middle only; while the evaporation continues around the moistened spot. If, therefore, we moisten with a liquid the surface of the evaporating skin at any point, the liquid is forced inwards by the external pressure.! Let us suppose any part of the skin to be rubbed with fat, the transpiration ceases at that part.| If now the skin around the part is in its normal activity, if, therefore, in the surrounding parts liquid is constantly passing off by evaporation, the fat must be urged, by the unequal pressure thus arising, towards these parts, or it is absorbed, just as water, in the apparatus, Fig. 12, is absorbed, when in con- sequence of evaporation a difference between the internal and external pressure has arisen. If the whole skin were covered with fat, the absorption would be effected by the pulmonary evaporation. The blistering of the skin, and the sun-burning, to which men are exposed at great elevations, arise from the extraordinary dryness of the air, the increased evaporation, and the pressure by which the fluids filling the vessels are forced towards the surface. Several causes contribute jointly to the appearance of the sweat, to the efflux of fluid, from the pores of the skin. One of these obviously depends on the velocity, which the fluid set in motion by evaporation or by a mechanical cause, acquires from the accelerated motion of the blood. In consequence of this velocity, the fluid moves out beyond the limits of the absorbing membrane or skin. The changes of the vital process, caused by the unequal distribution of fluid in the body in consequence of evaporation, are best seen in animals which live in water, in whom, therefore, the above explained cause of motion in the normal state does not act. When a fish is held immersed in water, so that the head is out of the water, while the rest of the body is covered, it dies in a few minutes. It dies exactly in the same way when head and gills are held in the water, and the body in air (MiLNE EDWARDS ;) in both cases, without loss of weight. This fact shows that even if the weight of the animal be kept unaltered by the absorp- tion of water through the body kept in that medium, yet the distribution of the fluids in the body does not take place in the proportion necessary for the preser- vation of their vital functions. The fish dies. It is hardly necessary to remind the reader, that the experiments described in the foregoing pages, in so far as they permit us to draw conclusions as to the cause of the motion of the juices of the animal body, agree in all respects with the observations made on plants by STEPHEN HALES more than 120 years since. || The experiments of HALKS on the mechanism of the motion of the sap, may stand as a pattern to all times of an exceilent method. That they remain, to this moment, unsurpassed in the domain of vegetable physiology, may be, perhaps, explained by the fact that they date from the age of NEWTON. They ought to be familiar to every vegetable physiologis^ In the beginning of his work, HALES describes the experiments which he made * The force urging the fluids towards the skin is equal to the difference of pressure acting on the skin. t Liquids placed on the skin are absorbed by the evaporatiou of other parts. I Effect of rubbing fat on a part of the skin or on the whole of it. $ Fishes die in air, because the distribution of the fluids is prevented. II Experiments of HALES on the motion of the sap in plants. EXPERIMENTS ON THE MOTION OF THE SAP OF PLANTS. 37 on the motion of the sap in plants in consequence of their evaporation in branches covered with foliage, in cut plants as well as in those still provided with roots. He shows by the following experiment the influence of the mechanical pressure of a column of water, with arid without the help of evaporation. To a branch of an apple tree bearing its twigs and leaves, HALES fastened, air- tight, a tube seven feet long. He kept the branch with its twigs and leaves immersed in a large vessel of water, and filled the tube with water. By the pres- sure of the column of water, water was forced into the branch, and in two days the water in the tube had sunk 14| inches. On the third day, he took the branch out of the water, and exposed it to free evaporation in the air. The water in the tube fell, in twelve hours, 27 inches. To compare the force with which water is driven through the vessels of the wood, by pressure alone, with that produced by pressure and evaporation, he joined an apple branch, 6 feet long, with leaves, and exposed to the air, with a tube 9 feet long, which was filled with water. From the pressure caused by the column of water, and by the evaporation going on at the surface of the twigs and leaves, the water fell (Xlth experiment,) in one hoifr, 36 inches. He now cut off the branch 13 inches below the tube, and placed the portion cut off (with the twigs and leaves) vertically in a vessel of water. This last absorbed, in 30 hours, 18 ounces of water, while the portion of wood remaining in connection with the tube, which was 13 inches long, only allowed 6 ounces of water to pass, and that under the pressure of a column of 7 feet of water. HALES shows in three other experiments, that the capillary vessels of a plant, alone, and in connection with the uninjured roots, are easily filled with water by capillary attraction, without, however, possessing the power of causing the sap to flow out and to rise in a tube attached.* The motion of the sap, concludes HALES, belongs to the evaporating surface alone ; he proves that it goes on in an unequal degree from the stem, the twigs, the flowers, and fruit, and that the effect of the evaporation stands in a fixed ratio to the temperature and hygrometic state of the air. If the air were moist, but little were absorbed ; the absorption was hardly perceptible on rainy days. He opens the second chapter of his Statistics with the following introduction : " Having in the first chapter seen many proofs of the great quantities of liquor imbibed and perspired by vegetables, I propose in this, to inquire by what force they do imbibe moisture. Though vegetables (which are inanimate) have not an engine, which by its alternate dilations and contractions, does in animals, forcibly drive the blood through the arteries and veins; yet has nature wonderfully contrived other means, most powerfully to raise and keep in motion the sap. In his experiment XXL, he exposed one of the chief roots of a pear tree in full growth at a depth of 2.j feet, cut off the point of it, and connected the part of the root left in connection with the stem with a tube which he filled with water and closed with mercury. In consequence of the evaporation from the surface of the tree, the root absorbed the water in the tube with such a force, that in six minutes the mercury rose to 8 inches in the tube. This corresponds to a column of water 9 feet high. This force is nearly equal to that with which the blood moves in the great femoral artery of the horse. HALES, in his experiment XXXIV., found the force of the blood in various animals; "By tying those several animals down alive upon their backs, and then laying open the great left crural artery, where it first enters the thigh, I fixed to it (by means of two brass pipes which run one into the other) a glass tube of above 10 feet long, and gth of an inch in diameter in bore. In which tube the blood of one horse rose 8 feet 3 inches, and the blood of another horse 8 feet 9 inches. The blood of a little dog 6| high." HALES showed by special experiments, that the absorbent force which he pointed out in the root is found also in the stem, in each separate twig, each leaf, and every part of the sumce ; and that the motion of the sap continues from the root towards * The motion of the sap is caused by the evaporating suriace. 38 MOTION OF THE JUICES OF THE ANIMAL BODY. the twigs and leaves, even when the stem has been entirely stripped of bark, inner and outer. This force acts not only from the roots in the direction of the summit but also from the summit in the direction of the root. From his experiment he deduces the presence of a powerful attractive force resi- ding in every part of the plant. We now know, that this attractive force, as such, did not cause the rise of the mercury or water in his tubes, and it appears clearly from his experiments, that the absorbent power of plants,, of each leaf, of each fibre of the root, is sustained by a powerful external force which is nothing else than the pressure of the atmos- phere.* By the evaporation of water at the surface of plants, a vacuum arises within them, in consequence of which water and matters soluble in water are driven inwards and raised from without with facility, and this external pressure, along with capillary attraction, is the chief cause of the motion and distribution of the juices.f With respect to the absorbent power of the plant for gases, under a certain exter- nal pressure, his experiments offer the most beautiful evidence*! HALES says, in his experiment XXII. , " This height of the mercury did in some measure show the force with which the sap was imbibed, though not near the whole force ; for while the water was imbibing, the transverse cut of the branch was covered with innumerable little hemispheres of air, and many air-bubbles issued out of the sap- vessels, which air did, in part, fill the tube e r, as the water was drawn out of it ; so that the height of the mercury could only be proportionable to the excess of the quantity of the water drawn off, above the quantity of air which issued out of the wood. And if the quantity of air which issued from the wood into the tube, had been equal to the quantity of water imbibed, then the mercury would not rise at all; because there would be no room for it in the tube. But if 9 parts of 12 in the water be imbibed by the branch, and in the mean time, but three such parts of air issue into the tube, then the mercury must needs rise near 6 inches, and so pro- portionably in different cases." When, in his experiments, the root, the stem, or a twig had been injured at any part, by the cutting off of buds, root-fibres, or small twigs, the absorbent power of the remainder was diminished in a very obvious degree (because, from these places, by the entrance of air the difference of air was more easily equalized ;) the absorbent power was greatest on freshly-cut surfaces, on which, however, it gra- dually decreased, till, after several days, it was not greater in these places than in the uninjured surface of the plant. The evaporation, further, argues HALES, is the powerful cause which provides food for the plant and its vicinity. Disease and death of the plant follow, when the proportion between evaporation and supply is interrupted or destroyed in any way. || When, in hot summers, the earth cannot supply, through the roots, the moisture which during the day has evaporated through the leaves and surface of the tree, when the tree, or a twig of it, dries up, the motion of the sap is arrested at these points. When once dried, capillary action alone cannot restore the original activity ; the evaporation is the chief condition of the life of plants ; by its means a permanent motion, a continually repeated change in the quality of the. juice (sap) is effected. " By comparing," says HALES, " the surface of the roots of plants, with the surface of the same plant above ground, we see the necessity of cutting off many branches from a transplanted tree : for if 256 square inches of root in surface was necessary to maintain this cabbage in a healthy natural state ; suppose upon dig- ging it up, in order to transplant, half the roots be cut off (which is the case of most young transplanted trees,) then it is plain that but half the usual nourishment can be carried up, through the roots, on that account ; and a very much less pro- * The pressure rf the atmosphere is the active force. 1 A partial vacuum is caused within plants by evaporation. j The surface of plants absorbs gases. $ The absorbent power diminished by injury to the plant. || Evaporation provides food for the plant. OBSERVATIONS OF HALES OX THE BLIGHT IN HOPS. 39 portion, on account of the small hemisphere of earth the new-planted, shortened roots occupy ; and on account of the loose position of the new-turned earth, which touches the roots at first but in few points." HALES proves the influence of suppressed evaporation by the following observa- tions on hop-vines. "Now there being 1,000 hills in an acre of hop-ground, and each hill having three poles, and each pole three vines, the number of vines will be 9,000 ; each of which imbibing four ounces, the sum of all the ounces, imbibed in an acre in a twelve hours' day, will be 30,000 ounces = 15,750,000 grains = 62,007 cubic inches, or 220 gallons ; which divided by 0,272,040, the number of square inches in an acre, it will be found, that the quantity of liquor perspired by all the hop- vines, will be equal to an area of liquor, as broad as an acre, and T J T part of an inch deep, besides what evaporated from the earth. And this quantity of moisture in a kindly state of the air is daily carried off in a sufficient quantity to keep the hops in a healthy state ; but in a rainy moist state of air, without a due mixture of dry weather, too much moisture hovers about the hops, so as to hinder in a good measure the kindly perspiration of the leaves, whereby the stagnating sap corrupts, and breeds mouldy fen, which often spoils vast quantities of flourishing hop-grounds." " This was the case in the year 1723, when ten or fourteen days' almost con- tinual rains fell, about the latter half of July, after four months' dry weather ; upon which the most flourishing and promising hops were all infected with mould or fen, in their leaves and fruit, whilst the then poor and unpromising hops escaped, and produced plenty ; because they, being small, did not perspire so great a quan- tity as the others ; nor did they confine the perspired vapor, so much as the large thriving vines did, in their shady thickets. This rain on the then warm earth made the grass shoot cut as fast as if it were in a hot-bed ; and the apples grew so preci- pitately, that they were of a very fleshy constitution, so as to rot more remarkably than had ever been remembered."* " The planters observe, that when a mould or fen has once seized any part of the ground, it soon runs over the whole ; and that the grass, and other herbs under the hops, are infected with it." " Probably because the small seeds of this quick-growing mould, which soon come to maturity, are blown over the whole ground. Which spreading of the seed may be the reason why some grounds are infected with fen for several years successively." " I have in July (the season for fire-blasts, as the planters call them) seen," says HALES, " the vines in the middle of a hop-ground all scorched up, almost from one end of a large ground to the other, when a hot gleam of sunshine has come immediately after a shower of rain ; at which time the vapors are often seen with the naked eye, but especially with reflecting telescopes, to ascend so plenti- fully, as to make a clear and distinct object become immediately very dim and tremulous. Nor was there any dry gravelly vein in the ground, along the course of this scorch. It was, therefore, probably owing to the much greater quantity of scorching vapors in the middle than outsides of the ground, and that being a denser medium, it was much hatter than a more rare medium." " This is an effect which the gardeners about London have too often found to their cost, when they have incautiously put bell-glasses over their cauliflowers early in a frosty morning, before the dew was evaporated off them ; which dew being raised by the sun's warmth, and confined within the glass, did there form a dense, transparent, scalding vapor, which burnt and killed the plants." When these observations are translated into our present language, we perceive with what acuteness and accuracy HALES recognized the influence of evaporation on the life of plants. According to him the development and growth of the plant depends on the supply of nourishment and moisture from the soil, which is determined by a certain * Observations of Hales on the blight in hops and other plants. 40 MOTION OF THE JUICES OF THE ANIMAL BODY. temperature and dryness of the atmosphere. The absorbent power of plants the motion of their sap, depends on evaporation ; the amount of food necessary for their nutrition, which is absorbed, is proportional to the amount of moisture given out (evaporated) in a given time. When the plant has taken up a maximum of moisture, and tha evaporation is suppressed by a low temperature or by continued wet weather, the supply of food, the nutrition of the plant, ceases; the juices stagnate, and are altered ; they now pass into a state in which they become a fertile soil for microscopic plants. When rain falls after hot weather, and is fol- lowed by great heat without wind, so that every part of the plant is surrounded by an atmosphere saturated with moisture, the cooling due to further evaporation ceases, and the plants are destroyed by fire-blast or schorching (Sonnenbrand, German, literally, sun-burn or sun-blight.) After the experience and observations of so long a period in reference to the influence of evaporation on the condition of plants, I hardly think that any un- prejudiced observer can entertain the smallest doubt concerning the cause of the great mischief which has befallen agriculture during the last few years.* If HALES, that unequalled observer and inquirer, had known the potato disease, I hardly "believe that he would have ascribed it to an internal cause belonging to the plant, any more than he thought of ascribing the blight of the hop plants, formerly men- tioned, to a special hop disease, or the rotting of the apples to an apple disease. Even PARMENTIER, to whom France is indebted for the introduction of the potato, knew this disease, and has very accurately described it.t The term " potato-rot " has been known to the oldest peasants and agriculturists since their youth ; it has, doubtless, only acquired of late years the frightful significance, which seems to threaten the well being of nations, siiice the causes, which formerly brought it locally into existence, have spread over whole districts and countries. The writings of HALES bring to our century from a preceding one the consoling certainty (and this is especially important,) that the cause of this decay is not to be looked for in a degeneration of the plant, but depends on the combination of certain conditions accidentally coincident ; and that these, when they are well ascertained and kept in view, enable the agriculturist, if not to annihilate, at least to diminish, their hurt- ful influence.! The potato plant obviously belongs to the same class of plants as the hop plant, namely, to that class which is most seriously injured by the stagnation of their juices in consequence of suppressed transpiration. According to KNIGHT, the tubers are not formed by swelling of the proper roots, but by the development of a kind of underground stalks or runners. He found that when the tubers under ground were suppressed, tubers were formed on the stalks above ground ; and it is conceivable that every external cause which exerts a hurtful influence on the healthy condition of the leaves and stalks, must act in like manner on the tubers. In the districts which were most severely visited by the so-called potato disease in 1846, damp, cold, rainy weather followed a series of very hot days ; and in 1847, cold and rain came on, after continued drought, in the beginning of September, exactly at the period of the most luxuriant growth of the potatoes. || In most places, no trace of disease was observed in the early potatoes before the middle of August ; and even after that period low-lying, cold and wet fields, were chiefly attacked by it. In many plants, in the same field, in which the seed pota- toes had been destroyed by putrefaction and decay, the tubers appeared quite healthy, while in others it was easy to see that these tubers alone, which lay next to the x)ld potatoes, were infected and attacked by the disease, and that on the side next to the old tubers. ^[ In 1846 all the potato plants in my garden died completely off towards the end of August, before a single tuber had been formed ; and in 1847, in the same field, * The potato blight has probably a similar origin. t The potato blight has been long known. J It is not due to a degeneration of the plant, but to a combination of external causes. i? The potato plant is one of those which suffers most from suppressed evaporation. || Character of the weather in 1840 and 1847, when the potato blight prevailed. if In most places the early potatoes escaped till after the middle of August. EFFECT OF COLD ON PLANTS. 41 the tubers of all those plants which stood under trees, and in protected spots, were quite rotten, while no trace of disease appeared in spots which were more elevated and more fully exposed to the current of air. The cause of the disease is the same which, in spring and autumn, excites influenza ; that is, the disease is the effect of the temperature and hygrometric state of the atmosphere, by which, in conse- quence of the disturbance of the normal transpiration, a check is suddenly, or for a considerable time, given to the motion of the fluids, which is one chief condition of life, and which thus becomes insufficient for the purposes of health, or even hurtful to the individual.* The whole existence of a plant, the resistance which it opposes to the action of the atmospheric oxygen, is most closely connected with the continued support of its vital functions. The mere alternation of day and night makes, in this respect, a great difference. The sinking of the external temperature by B. few degrees, causes the leaves to fall in autumn ; and a cold night is followed by the death of many annual plants. If we reflect that a plant, in order to protect itself from external causes of disturbance, or to seek the food which it requires, cannot change its place ; that its normal vital functions depend on the simultaneous and combined action of water, of the soil, of the external temperature, and of the hygrometric state of the atmosphere ; that is, on four external circumstances ; it is easy to comprehend the disturbance of functions which must occur in the organism in consequence of any change in the mutual relations of so many combined agencies.t The state of a plant is a sure indication of equilibrium or misproportion in the external conditions of its life ; and the dexterity of the accomplished gardener consists exactly in this, that he knows and can establish the just proportion of these conditions for each species of vegetable. Only one of these numerous conditions is in the power of the agriculturist, and that is, the production of the quality of the soil appropriate for the crop, including the necessary modification of its composition, by the mechanical working of the soil ; by the irrigation or draining of the fields ; and lastly, by the employment of manure. When one of the constituents of the soil, which, under the given circumstances, is necessary for the support of the vital func- tions, is absent, the external injurious influence is strengthened by this deficiency. Had this constituent been present, the plant would have been enabled to oppose to the external hurtful influences a continued resistance. One day may be decisive as to the life or death of a plant.J An accurate knowledge of the influence exerted by the various constituents of the soil on the diseased condition, must enable the agriculturist to protect and preserve many of his fields for a long time from this destruction ; but it is obvious that a universal remedy against this evil does not exist. When the vessels of the plant are filled to overflowing with water, and the motion of the sap is suppressed, the nutrition, in most plants, is arrested, and death takes place. Every one knows the effect of a sudden or of a gradual overfilling of certain parts or organs, when the corresponding evaporation is suppressed. By the endos- motic pressure of the water flowing towards those cells, which contain sugar, mucilage, gum, albumen, and soluble matters in general, the juicy fruits and seeds approaching maturity burst, ami the juice of grapes, cherries, plums, &c., passes, on contact with the air, into a state of progressive change. The fungi which have been observed on the potato plants and the putrefaction of the tubers, are not the signs of a disease, but the consequences of the death of the plant. Among the most important of the experiments made by Hales we must reckon undoubtedly those on the rise of the spring sap in perennial plants. His observa- tions have been entirely confirmed by all those who since his time have studied the subject; but, in my opinion, without our having approached one step nearer to the cause of the phenomena. * The cause of potato blight is the same as that of influenza, and depends on the temperature and hygrometric state of the air. t The life of plants is dependent chiefly on four external causes : only one of which, namely, the quality of the soil, in the power of the agriculturist. J Effects of the presence or absence of a single constituent of the soil. $ The plant dies, and fungi and putrefaction follow. 6 42 MOTION OF THE JUICES OF THE ANIMAL BODY. The most recent experiments on this subject by E. BRUCKE, leave no doubt in regard to the actual state of our knowledge. According to DUTROCHET, it is the extremities of the radical fibres, called by DE CANDOLLE, spongioles, which effect the rise of the spring sap ; and he believes (L'agent immediat du mouvement vital, Paris,1826,) that the force with which the sap is driven upwards, acts from the root. DUTROCHET cut off a peice of a vine stem, two metres long; and he saw that the sap flowed steadily from the shortened stem in connection with the root. When he had again cut it off close to the ground, he observed the portion in the ground continued to pour forth sap from the whole cut surface, He pursued the experiment, going deeper every time, and he always found that the sap flowed from the part left in the ground, till at last he came to the extreme points of the fibres, in which he then located the origin of the moving force. The peculiar activity of the spongioles must, according to DUTROCHET, be ascribed to all the causes, taken together, which determine the phenomena of endosmosis. Now that we are better acquainted with the phenomena of what is called endos- mosis, we may oppose to this view some well founded doubts. All observers agree, that the increase in volume of a liquid, separated from another liquid by a porous diaphragm, is determined by a difference in the qualities of the two liquids. If their composition and properties be the same, there is no cause sufficent to produce mixture and change of volume, since in this case, the attraction of both for the diaphragm, and for each other, is perfectly equal. In the course of his admirable researches, BRUCKE determined the specific gravity of the spring sap which had flowed from the vine.* He found it, in one plant, = 1-0008, and in another, = 1-0009. (*) These numbers prove irresistably, that in the specific gravity of the sap of the vine is in no way different from that of ordinary spring water, or of the water which has filtered through garden mould. In most cases, spring water contains even more dissolved matter. The spring sap of the vine, which had the sp. g. 1-0008, raised a column of mercury to the height of 174 lines (14-5 inches,) and therefore exerted a pressure equal to that of a column of water 195 inches high. It is quite impossible to account for this pressure by the difference in the amount of dissolved matter in the water absorbed by the roots, and the sap flowing from the cut surface. In the experiment No. IX., of BRUCKE, made with a vine, the sap of which had the sp. g. 1-0009 the mercury was raised at 7 A. M., to the height of 209 lines, (nearly 17-5 inches. No one can doubt that what is called endosmosis has some share in the rise of the sap of the maple and birch trees, which is proportionally rich in sugar, and differs materially in composition from spring water, as well as on the flow or exu- dation of gummy or saccharine juices; but the pressure exerted in these cases, cannot be compared to that exerted by the sap of the vine, where the causes included under the word endosmosis cannot act. It is evident, that the cause of the pressure of the spring sap must be transient, called into action by external causes, and limited to a short period.! The experi- ment of DUTROCHET, from which he concludes that the cause of the rise of the sap resides in the extreme points of the roots, may be thus interpreted : " The cause of the efflux and pressure of the sap exists in all parts of the uninjured plant, down to the extreme spongioles of the root." The present season does not admit of experiments on this point ; but as spring approaches, it may be proper here to develope more clearly the grounds of the opinion, that the cause of the efflux of the sap of the vine is a transient one. Per- haps some one may thus be induced to decide experimentally all the questions of this remarkable phenomenon. (') Poggendorf's Annalen der Physik, briii. 177. * Observations of BRUCKE on the specific gravity of the sap of vines. f The cause of the rise of the sap is transient ; and depends on external influences EXPERIMENTS OF HALES. 43 HALES, in his experiment XXXIV., cut off a vine stem 7 feet above the ground, and attached to the trunk tubes of 7 feet long, joined together. Below the cut there were no branches. This was done on the 30th of March, at 3 P.M. As the stem poured out no sap on that day, he poured water into the attached tube to the height of two feet. This water was absorbed by the stem, so that about 8 P.M., the water had fallen to 3 inches in the tube. The next day, 3 past 6 A. M., the sap stood 3 inches higher than at 8 the evening before. From this time the sap continued to rise, till it reached a 'height of 21 feet. It would perhaps, says HALES, have risen higher, had the joinings of the tubes been more water-tight. Whatever opinion we may entertain as to the cause of the efflux and pressure of the sap, it is impossible to suppose that the mechanical or any other structure or quality of the radical fibres, the spongioles, or the inner parts of the vine stem generally, can have changed so much between the evening of the 30th and the morning of the 31st, as to give rise to two completely opposite influences. On the evening of the 30th the water poured into the tube was absorbed ; on the 31st it was expelled with a continually increasing force. In his experiment XXXVII., HALES fixed, on three branches of a horizontally trained espalier vine, siphon tubes, filled to a certain point with mercury. The three branches received their sap from the common stem, that stem from the root. The first branch was 7 feet from the second, the second 22 feet 9 inches from the third. The first and third branches were two years old, the middle one was older. From the 4th to the 20th of April, the mercury stood, in consequence of the pressure of the sap, higher in the open limb of the tubes than in the other which was attached to the branch. The greatest height attained by the mercury was from 21 to 26 inches. On the 21st of April, when the flowering was nearly over, the sap in the middle branch went backwards ; it was absorbed, and so considerably, that the mercury stood 4 inches lower in the open limb than in the other. After a rainy night on the 24th of April, the sap again rose in the open tube 4 inches. In the first (lowest) branch, the sap went back on the 29th of April, 9 days after the middle one ;. the third (highest) branch only began to absorb the sap on the 3d of May, thirteen days after the middle one. We see from this experiment, as HALES observes, " That the cause which produces the flow of the sap does not proceed from the root alone, but that it belongs to a force inherent in the stem and branches. For the middle branch followed more rapidly the changes of temperature, of dryness and of moisture, than the two others, and absorbed the sap nine days before one, and thirteen days before the other, both of which, during this time, poured out sap instead of absorbing it. (The cause of the efflux and pressure had, in the older branch, dis- appeared, and given place to an opposite influence, while it still continued active in the two younger branches.) " The middle branch was 3 feet 8 inches higher than that next the stem. The height of the mercury in the three tubes was, respectively, 14s, 12, and 13 inches. The maximum was 21, 26, and 26 inches. These numbers prove that the greater length of the middle branch had no perceptible influence on the height of the mercury, as compared with that in the other tube." In his experiment XXXVIII., HALES observes, " Moisture and warmth made the sap most vigorous. If the beginning or middle of the bleeding season, being very kindly, had made the motion of the sap vigorous, that vigor would imme- diately be greatly abated by cold easterly winds.* " If in the morning while the sap is in a rising state, there was a cold wind with a mixture of sunshine and cloud ; when the sun was clouded the sap would immediately visibly subside, at the rate of an inch in a minute for several inches, if the sun continued so long clouded ; but as soon as the sunbeams broke out * Effect of cold and of shade on the rise of the sap. 44 MOTION OF THE JUICES OF THE ANIMAL BODY. again, the sap would immediately return to its then rising state, just as any liquor in a thermometer rises and falls with the alternacies of heat and cold ; whence it is probable, that the plentiful rise of the sap in the vine in the bleeding season, is effected in the same manner." If we consider, that the sap in spring, even with a clouded sky, does not cease to rise and flow, for this even goes on during the night, we cannot explain the fall of the sap from the moment that the sun was covered by a cloud by a mere change of temperature in the juice, because the time was too short for the cooling and contraction by cooling (one inch in a minute.)* Heat determined the more rapid rise, and cold the fall, but they acted on a cause which lay higher than the root, and which was more sensitive to heat than the liquid itself.' HALES says, in his experiment XXXVIII. " In very hot weather many air bubbles would rise, so as to make a froth an inch deep, on the top of the sap in the tube.t " I fixed a small air pump to the top of a long tube, which had twelve feet height of sap in it ; when I pumped, great plenty of bubbles arose, though the sap did not rise, but fell a little, after I had done pumping." In his experiments on the amount of air absorbed by plants, chapter V., he observes, " in the experiments on vines, the very great quantity of air which was continually ascending from the vines, through the sap in the tubes ; which mani- festly shows what plenty of it is taken in by vegetables, and is perspired off with the sap through the leaves." When we take these facts into consideration, the opinion appears not untenable, that the incomprehensible force, which causes the sap of the vine to flow in spring, may be simply referred to a disengagement of gas which takes place in the capillary vessels (filled with liquid, and keeping themselves constantly full,) in consequence of a kind of germination ; and it is possible that the height of the column of mercury, or of water, is only a measure of the elasticity of the dis- engaged gas 4 Let us suppose a strong glass bottle, in the mouth of which a long tube, open at both ends, and reaching to the bottom, is cemented, to be filled with a liquid in which, from any cause, a gas is disengaged (solution of sugar mixed with yeast, for example,) it is evident that the liquid must rise in the tube from the separation of the gas. When it has risen to 32 feet, the gas will occupy only the half, and at 64 feet, one third of its volume under the usual atmospheric pressure. In this case, the height of the liquid in the tube is no measure of a special power residing in the walls of the vessel ; it only shows the tension of the gas. If the walls of the vessel were permeable to the gas under a certain pressure, no further rise, beyond that point, could occur. If, in the apparatus, Fig. 4, we push the tube a through the cork down to the little lead drop ; if we then fill the tube c with water to which some yeast has been added, and a with solution of sugar, and expose the whole to a temperature of from 68 to 75, the liquid rises in b, from the gas disengaged in c, very rapidly, so as to overflow. If c be filled with solution of sugar, and a with yeast, the same rise occurs, and lasts till the disengaged gas puts an end to the contact between the membrane and the liquid. It is hardly necessary to point out, that the idea above expressed as to the cause of the flow and pressure of the spring sap, is nothing more than an indication of the direction in which experiments must be made. When we know with accuracy the volume of the liquid which flows out of a vine at the time of flowering, and the quantity of gas which is developed at the same time, we shall, I trust, find ourselves a step nearer to the explanation of this phenomenon. According to the experiments of GEIGER and PROUST, the sap of the vine is rich in carbonic acid ; and it is possible that the gas which is disengaged, may be no other than carbonic acid gas. * How is this effect to be accounted for ] t Gas is given off with the sap. J The rise of the sap may, therefore, be caused by th evolution of gas. APPENDIX. ON THE NATURE AND PREVENTION OF THE POTATO DISEASE. AFTER the preceding pages were in print, I received from Baron Liebig a copy of the Journal of the Agricultural Association of the Grand Duchy of Hesse, (Darmstadt,) No. 7, dated 15th February, 1848, containing the account of a method proposed by Dr. Klotzsch (Keeper of the Royal Herbarium, Berlin, and a distinguished Botanist and Vegetable Physiologist,) for preventing the ravages of the potato disease. The proposal of Dr. Klotzsch, and his views as to the nature of the disease, are such as materially to strengthen the opinions expressed on this subject by Baron Liebig, (see pp. 87, seq.) As a knowledge of the method suggested by Dr. Klotzsch is likely to be interesting to many of the readers of this work, I have thought it right to give it in an Appendix. WILLIAM GREGORY. METHOD PROPOSED BY DR. KLOTZSCH, FOR THE PROTECTION OF THE POTATO PLANT AGAINST DISEASES. The potato, which is an annual plant, represents, in the tubers developed from the stem, the perennial part of a plant. For while the duration of its development is analogous to that of annuals, its functions coincide exactly with those of dicotyle- donous shrubs and trees. " The potato plant differs from all those plants which are cultivated for economical purposes in Europe, and can only be compared to those orchideous plants which yield salep, and which are not yet cultivated among us. " The tubers, both of the potato and of the salep plants, are nutritious, and agree in this, that in the cells of the tubers, grains of starch, with more or less azotized mucilage, are collected, while the cell walls possess the remarkable property of swelling up into a jelly, and thus becoming easily digestible, when boiled with water. " But while the tuber of salep contains only one bud, or germ, the potato usually develops several, often many, germs. " The potato plant, like all annuals, exerts its chief efforts in developing flowers and fruit. Like all annuals, too, it has the power of shortening this period of development, when the power of the roots is limited ; as also of lengthening it when the extent and power of the roots are increased. We observe in nature that plants with feebly developed roots often have a weak, sickly aspect, but yet come to maturity in flower and fruit sooner than stronger individuals, well furnished with roots. (45) 46 APPENDIX. 11 In perennial plants we observe a second effort, which is directed towards preparing and storing up nutritious matter, for the consumption of the plant. The preparation of this nutriment is effected by the physiological action of the leaves, under the influence of the roots. The stronger and larger the former are, the more is this preparation of food delayed. " The nutritious matters are stored in the colored stratum of the bark in shrubs and trees, and in the tubers in the potato and salep plants. Not only, however, the nutrient matters, but also the cells, owe their origin to the physiological action of the leaves. * On considering these things, it follows, that the potato plant requires more care than is usually devoted to it. Hitherto the whole cultivation consisted in clearing off weeds, and hoeing up the earth round the stems. Both of these measures are, indeed, necessary, but they are not alone sufficient ; for the plant is cultivated, not on account of its fruit, but for the sake of its tubers, and our treat- ment should be modified accordingly. " The chief points to be attended to, with a view lo the attainment of this object, namely, the increase of tubers, are 1. To increase the power in the roots, and 2. To check the transformation which occurs m the leaf. " We obtain both ends simultaneously, if, in the 5th, 6th, and 7th week after setting the tubers, and in the 4th and 5th week after planting out germs furnished with roots, or at a time when the plants reach the height of 6 to 9 inches above the soil, we pinch off the extreme points of the branches or twigs to the extent of half an inch downwards,* and repeat this on every branch or twig, in the 10th and llth week, no matter at what time of day. " The consequences of this check to the development of the stem and branches, is a stimulous to the nutrient matters in the plant in the direction of the increase, both of roots and of the multiplication of the branches of the stem above ground, which not only favors the power of the root, but also strengthens the leaves and stalks to such a degree, that the matters prepared by the physiological action of these parts are increased and applied to the formation of tubers, while at the same time the direct action of the sun's rays on the soil is prevented by the thick foliage, and thus the drying up of the soil and its injurious consequences are avoided. The checking of the transformation in the leaf is equivalent to the interruption of the natural change of the leaves into calyces, corollae, stamens, and pistils, which is effected at the expense of the nutrient matters collected in the plant ; and these, when this modification of the leaves is arrested, are turned to account in the forma- tion of tubers. " Led by these views, I made, in 1846, experiments on single potato plants, carefully marked by pinching off the ends of the branches. They were so readily distinguished in their subseqnent growth from the plants beside them, by more numerous branches, larger and darker foliage, that in truth no marking was neces- sary. " The produce from these plants of tubers was abundant, and the tubers were perfectly healthy ; while the plants next them which had not been so treated, gave uniformly less produce, at the same time the tubers were rough on the surface, and in many instances attacked with the prevailing disease. This experiment was incomplete, and did not give a positive result, but it was yet encouraging for me. " In the middle of April, 1847, an experiment was made on a low-lying field with the round white potatoes, generally cultivated here, a variety which had not suffered much from the disease which first appeared here 1845. The potatoes were planted in the usual way by an experienced farm servant. " After weeding them in the end of May, I renewed my experiment by pinching off the points of the branches of every second row, and repeated this in the end of June. The result surpassed all expectations. The stocks of the plants not treated * Any one would be bitterly disappointed, who on the principle, that " there cannot be too much of a good thing," should take off more than is here recommended, in order to use it as fodder. APPENDIX. 47 oil my plan, were long, straggling, and sparingly furnished with leaves, the leaves themselves, small and pale green. " In the next field, potatoes of the same variety were planted on the same day and left to nature. They appeared in the first six weeks healthy, even strong, but gradually acquired a poor aspect as the time of flowering and fruit approached, and finally, exhibited precisely the same appearance as the rows not treated by pinching off the extremities in the field in which my experiments were made. " The harvest began in the surrounding fields in the middle of August, and was very middling. The tubers were throughout smaller than usual, very scabby, and within these fields, to a small extent, attacked by the wet rot. ** In the end of August, the difference between the rows treated by me and those not treated, became so striking that it astonished all the work people in the neigh- borhood, who were never tired of inquiring the cause. The stocks of the rows left to themselves were all now partly dried, partly dead. On the contrary, the rows treated as above were luxuriant and in full vigour, the plants bushy, the foliage thick, the leaves large and green, so that most people suppposed they had been later planted. "But the difference in the tubers was also very decided. The tubers of the plants in the rows treated on my plan were not, indeed larger, but vastly more numerous, and they were neither scabby nor affected with any disease whatever. A few had pushed (which was to be ascribed to a late rain,) and were apparently incompletely developed, while scab and wet rot attacked more and more the tubers of the other plants, which also fell off on the slightest handling. " Although I am far from believing that I am able to explain the nature of the potato disease which has visited us of late years, yet I feel certain that I have dis- covered a means of strengthening the potato plant to such a degreea s to enable it to resist the influences which determine such diseases. * Should any one be deterred from continuing the cultivation of potatoes, on account of the manipulation here recommended, which may be performed by women and even by children, I would remind him that the same field planted with potatoes is capable of supplying food to twice as many persons as when employed to growing wheat." From the Annals of Agriculture in Prussia, edited by the College of Rural. Economy. DR. KLOTZSCH presented to the King of Prussia a memorial offering to give to the world his method of preventing disease in potatoes, provided he were assured of a remuneration of 2,000 dollars, (about 36300,) if, after three years experience it should be found efficacious. The King handed the memorial to the Minister of the Interior, who requested the College of Rural Economy to discuss the matter with Dr. Klotzsch. The president of the college undertook the arrangement, and, after Dr. Klotzsch had explained to him privately his method, reported most favorably of it to the College, which unanimously recommended that the very moderate remuneration asked for by Dr. Klotzsch should be secured to him on the following conditions, which were accepted by him. 1. That the College of Rural Economy should be the judges of the efficacy of the proposed method. 2. That their decision should be given, at latest, within three years, provided the potato disease against which the plants are to be protected, should appear during that period. The Minister of the Interior approved of the recommendation, and authorized the College to conclude an agreement with Dr. Klotzsch. The agreement has been concluded, and now the method is published that it may be tried and tested as widely as possible by comparative experiments, similar to those made by Dr. Klotzsch himself. The cost of it is stated not to exceed Is. 6d. per acre in Germany. It is very desirable that this method should be tried in the British Islands, and as the season for trying it now approaches, I have here given Dr. Klotzch's account entire. WILLIAM GREGORY. THE END. 4 . CHEMISTRY IN ITS APPLICATION TO AGRICULTUKE AND PHYSIOLOGY, BY JUSTUS LIEBIG, M.D., PH.D. F.R.S., M.R.I.A., PROFESSOR OF CHEMISTRY IN THE UNIVERSITY OF GIESSEN ; KNIGHT OF THE HESSIAN ORDER, AND OF THE IMPERIAL ORDER OF SAINT ANN ; MEMBER OF THE ROYAL ACADEMY OF SCIENCES OF STOCKHOLM ; CORRESPONDING MEMBER OF THE ROYAL ACADEMIES OF SCIENCES OF BERLIN AND MUNICH ; OF THE IMPERIAL ACADEMY OF ST. PETERSBURGH J OF THE ROYAL INSTITUTION OF AMSTERDAM, ETC. ETC. EDITED FROM THE MANUSCRIPT OF THE AUTHOR BY LYON PLAYFAIR, PH.D. FROM THE LAST LONDON EDITION, MUCH IMPROVED. T. B. PETERSON, No. 98 CHESNUT STREET. TO THE BRITISH ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE. ONE of the most remarkable features of modern times is the combination of large numbers of individuals representing the whole intelligence of nations, for the express purpose of advancing science by their united efforts, of learning its pro- gress, and of communicating new discoveries. The formation of such associations is, in itself an evidence that they were needed. It is not every one who is called by his situation in life to assist in extending the bounds of science ; but all mankind have a claim to the blessings and benefits which accrue from its earnest cultivation. The foundation of scientific institutions is an acknowledgment of these benefits, and this acknowledgment proceeding from whole nations may be considered as the triumph of mind over empiricism. Innumerable are the aids afforded to the means of life, to manufactures and to commerce, by the truths which assiduous and active inquirers have discovered and rendered capable of practical application. But it is not the mere practical utility of these truths which is of importance. Their influence upon mental culture is most beneficial ; and the new views acquired by the knowledge of them enable the mind to recognise, in the phenomena of nature, proofs of an Infinite Wisdom, for the unfathomable profundity of which, language has no expression. At one of the meetings of the chemical section of the " British Association for the Advancement of Science," the honourable task of preparing a Report upon the state of Organic Chemistry was imposed upon me. In the present work I present the Association with a part of this report. I have endeavoured to develope, in a manner correspondent to the present state of science, the fundamental principles of Chemistry in general, and the laws of Organic Chemistry in particular, in their application to Agriculture and Physiology ; to the causes of fermentation, decay, and putrefaction ; to the vinous and acetous fermentations, and to nitrification. The conversion of woody fibre into wood and mineral coal, the nature of poisons, contagions, and miasms, and the causes of their action on the living organism, have been elucidated in their chemical relations. I shall be happy if I succeed in attracting the attention of men of science to subjects which so well merit to engage their talents and energies. Perfect Agri- culture is the true foundation of all trade and industry it is the foundation of the riches of states. But a rational system of Agriculture cannot be formed without the application of scientific principles ; for such a system must be based on an exact acquaintance with the means of nutrition of vegetables, and with the in- fluence of soils and action of manure upon them. This knowledge we must seek 3 IV PREFACE. from chemistry, which teaches the mode of investigating the compostion and of studying the characters of the different substances from which plants derive their nourishment. The chemical forces play a part in all the processes of the living animal organ- ism ; and a number of transformations and changes in the living body are exclu- sively dependent on their influence. The diseases incident to the period of growth of man, contagion and contagious matters, have their analogues in many chemical processes. The investigation of the chemical connection subsisting between those actions proceeding in the living body, and the transformations presented by chemical compounds, has also been a subject of my inquiries. A perfect exhaustion of this subject, so highly important to medicine, cannot be expected without the co-opera- tion of physiologists. Hence I have merely brought forward the purely chemical part of the inquiry, and hope to attract attention to the subject. Since the time of the immortal author of the "Agricultural Chemistry," no chemist has occupied himself in studying the applications of chemical principles to the growth of vegetables, and to organic processes. I have endeavoured to follow the path marked out by Sir Humphry Davy, who based his conclusions only an that which was capable of inquiry and proof. This is the path of true philoso- phical inquiry, which promises to lead us to truth the proper object of our research. In presenting this report to the British Association I feel myself bound to convey my sincere thanks to Dr. Lyon Plairfair, of St. Andrew's, for the active assistance which has been afforded me in its preparation by that intelligent young chemist, during his residence in Giessen. I cannot suppress the wish that he may succeed in being as useful, by his profound and well grounded knowledge of chemistry, as his talents promise. JUSTUS LIEBIG. Gi'ssen, September 1, 1840. EDITOR'S PREFACE. THE former edition of this work was prepared in the form of a report on the present state of Organic Chemistry. The state of a science such as this could not be exhibited by a systematic treatise on organic compounds, but by showing that the science was so far advanced as to be useful in its practical applications. The work was written by a Chemist, and addressed to Chemists. The author did not flatter himself that his opinions would be so eagerly embraced by agricul- turists, as circumstances have shown to be the case. Hence his language and style were less adapted for them than for those who are conversant with the abstract details of chemical science. But the eager reception of the work by agriculturists has shown that they possess an ardent desire to profit by the aids offered to them by Chemistry. It, therefore, became necessary to adapt the work for those who have not had an opportunity of making that science a peculiar object of study. The Editor has endeavoured to effect this change. In doing so, it was necessary to retain the original character of the work ; hence those alterations only have been made which are calculated to render the work more generally useful. It must be remembered that the object of the author was not to write a " System of Agricultural Chemistry," but to furnish a " Treatise on the Chemistry of Agricul- ture." It is to be hoped that those who are acquainted with the general doctrines of Chemistry will find no difficulty in comprehending any of the principles here developed. The author has enriched the present edition with many valuable additions; allusion may be particularly made to the practical illustration of his principles furnished in the Supplementary Chapter on Soils. The analyses of soils contained in that chapter will serve to point out the culpable negligence exhibited in the examination of English soils. Even in the analyses of professional chemists, published in detail, and with every affectation of accuracy, the estimation of the most important ingredients is neglected. How rarely do we find phosphoric acid among the products of their analyses ? potash and soda would appear to be absent from all soils in the British territories ! Yet these are invariable constituents of fertile soils, and are conditions indispensable to their fertility. Primrose, November 22, 1841: 6 CONTENTS. PAGE Object of the Work .......... 9 PART FIRST. ON THE CHEMICAL PROCESSES IN THE NUTRITION OF VEGETABLES. "T On the Constituent Elements of Plants ..... 10 II. On the Assimilation of Carbon ...... 12 III. On the Origin and Action of Humus ..... 23 IV. On the Assimilation of Hydrogen ..... 27 V. On the Origin and Assimilation of Nitrogen .... 30 VI. On the Inorganic Constituents of Plants .... 36 VII. The Art of Culture ...... 43 VIII. On the Alternation (Rotation) of Crops .... 54 IX. On Manure .......... 59 Supplementary Chapter. On the Chemical Constituents of Soils . 70 Appendix to Part 1 ........... 84 PART SECOND. ON THE CHEMICAL PROCESSES OP FERMENTATION, DECAY, AND PUTREFACTION. CHAPTER I. Chemical Transformations ....... 87 II. On the Causes which effect Fermentation,. Decay, and Putre- faction .......... 88 III. Fermentation and Putrefaction ...... 90 IV. On the Transformation of Bodies which do not contain Nitro- gen as a constituent, and of those in which it is present 92 V. Fermentation of Sugar ..... . . . 95 VI. Eremacausis, or Decay ....... 98 VII. Eremacausis of Bodies destitute of Nitrogen: Formation of Acetic Acid ......... 100 VIII. Eremacausis of Substances containing Nitrogen: Nitrification 102 IX. On Vinous Fermentation : Wine and Beer . . ' . . 103 X. On the Decay of Woody Fibre ...... 110 XL On Vegetable Mould ........ 112 XII. On the Mouldering of Bodies : Paper, Brown Coal, and Mi- neral Coal ......... 112 XIII. On Poisons, Contagions, and Miasms . . .115 Appendix to Part II ........ . 129 Index ... 131 ORGANIC CHEMISTRY IN ITS APPLICATION TO VEGETABLE PHYSIOLOGY AND AGRICULTURE. THE object of Chemistry is to examine *nto the composition of the numerous modifi- cations of matter which occur in the organic and inorganic kingdoms of nature, and to investigate the laws by which the combina- tion and decomposition of their parts is effected. Although material substances assume a vast variety of forms, yet chemists have not been able to detect more than fifty-five bodies which are simple, or contain only one kind of matter, and from these all other substances are produced. They are con- sidered simple only because it has not been proved that they consist of two or more parts. The greater number of the elements occur in the inorganic kingdom. Four only are found in organic matter. But it is evident that this limit to their number must render it more difficult to as- certain the precise circumstances under which their union is effected, and the laws which regulate their combinations. Hence chemists have only lately turned their at- tention to the study of the nature of bodies generated by organized beings. A few years have, however, sufficed to throw much light upon this interesting depart- ment of science, and numerous facts have been discovered which cannot fail to be of importance in their practical applica- tions. The peculiar object of organic chemistry is to discover the chemical conditions essen- tial to the life and perfect development of animals and vegetables, and generally to in- vestigate all those processes of organic nature which are due to the operation of chemical laws. Now, the continued exist- ence of all living beings is dependent on the reception by them of certain substances, which are applied to the nutrition of their Crame. An inquiry, therefore, into the con- ditions on which the life and growth of living beings depend, involves the study of those substances which serve them as nutri- ment, as well as the investigation of the sources whence these substances are derived. 2 and the changes which they undergo in the process of assimilation. A beautiful connection subsists between the organic and inorganic kingdoms of na- ture. Inorganic matter affords food to plants, and they, on the other hand, yield the means of subsistence to animals. The conditions necessary for animal and veget- able nutrition are essentially different. An animal requires for its development, and for the sustenance of its vital functions, a cer- tain class of substances which can only be generated by organic beings possessed of life. Althougii many animals are entirely carnivorous, yet their primary nutriment must be derived from plants ; for the animals upon which they subsist receive their nour- ishment from vegetable matter. But plants find new nutritive material only in inorganic substances. Hence one great end of veget- able life is to generate matter adapted for the nutrition of animals out of inorganic substances, which are not fitted for this pur- pose. Now the purport of this work is, to elucidate the chemical processes engaged in the nutrition of vegetables. The first part of it will be devoted to the examination of the matters which supply the nutriment of plants, and of the changes which these matters undergo in the living organism. The chemical compounds which afford to plants their principal constituents, viz., carbon and nitrogen, will here come under consideration, as well as the relations in which the vital functions of vegetables stand to those of the animal economy and to other phenomena of nature. The second part of the work will treat of the chemical processes which effect the complete destruction of plants and animals after death, such as the peculiar modes of decomposition, usually described as fermen- tation, putrefaction, and decay; and in this part the changes which organic substances undergo in their conversion into inorganic compounds, as well as the causes which determine these changes, will become matter of inquiry. 9 PAET I. OF THE CHEMICAL PROCESSES IN THE NUTRITION OF VEGETABLES. CHAPTER I. OF THE CONSTITUENT ELEMENTS OF PLANTS. THE ultimate constituents of plants are those which form organic matter in general, namely, Carbon, Hydrogen, Nitrogen, and Oxygen. These elements are always pre- sent in plants, and produce by their union the various proximate principles of which they consist. It is, therefore, necessary to be acquainted with their individual charac- ters, for it is only by a correct appreciation of these that we are enabled to explain the functions which they perform in the veget- able organization. Carbon is an elementary substance, en- dowed with a considerable range of affinity. With oxygen it unites in two proportions, forming the gaseous compounds known under the names of carbonic acid and car- bonic oxide. The former of these is emit- ted in immense quantities from many vol- canoes and mineral springs, and is a product of the combustion and decay of organic matter. It is subject to be decomposed by various agencies, and its elements then ar- range themselves into new combinations. Carbon is familiarly known as charcoal, but in this state it is mixed with several earthy bodies ; in a state of absolute purity it con- stitutes the diamond. Hydrogen is a very important constituent of vegetable matter. It possesses a special affinity for oxygen, with which it unites and forms water. The whole of the phenomena of decay depend upon the exercise of this affinity, and many of the processes engaged in the nutrition of plants originate in the attempt to gratify it. Hydrogen, when in the state of a gas, is very combustible, and the lightest body known; but it is never found in nature in an isolated condition. Water is the most common combination in which it is presented; and it may be re- moved by various processes from the oxygen, with which it is united in this body. Nitrogen is quite opposed in its chemical characters to the two bodies now described. Its principal characteristic is an indifference to all other substances, and an apparent re- luctance to enter into combination with them. When forced by peculiar circum- stances to do so, it seems to remain in the combination by a vis inerlice; and very slight forces effect the disunion of these feeble compounds. Yet nitrogen is an invariable constituent of plants, and during their life is subject to the control of the vital powers. But when the mysterious principle of life has ceased to exercise its influence, this element re- sumes its chemical character, and materially assists in promoting the decay of vegetable matter, by escaping from the compounds of which it formed a constituent. Oxygen, the only remaining constituent of organic matter, is a gaseous element, which plays a most important part in the economy of nature. It is the agent em- ployed in effecting the union and disunion of a vast number of compounds. It is supe- rior to all other elements in the extensive range of its affinities. The phenomena of combustion and decay are examples of the exercise of its power. Oxygen is the most generally diffused element on the surface of the earth ; for, besides constituting the principal part of the atmosphere which surrounds it, it is a com- ponent of almost all the earths and minerals found on its surface. In an isolated state it is a gaseous body, possessed of neither taste nor smell. It is slightly soluble in water, and hence is usually found dissolved in rain and snow, as well as in the water of running streams. Such are the principal characters of the elements which constitute organic matter; but it remains for us to consider in what form they are united in plants. The substances which constitute the prin- cipal mass of every vegetable are com- pounds of carbon with oxygen and hydro- gen, in the proper relative proportions for forming water. Woody fibre, starch, sugar, and gum, for example, are such compounds of carbon with the elements of water. In another class of substances containing car- bon as an element, oxygen and hydrogen are again present ; but the proportion of oxygen is greater than would be required for produc- ing water by union with the hydrogen. The numerous organic acids met with in plants belong, with few exceptions, to this class. A third class of vegetable compounds contains carbon and hydrogen, but no oxy- gen, or less of that element than would be required to convert all the hydrogen into water. These may be regarded as com- pounds of carbon with the elements of water, and an excess of hydrogen. Such are the volatile and fixed oils, wax, and the resins. Many of them have acid characters. The juices of all vegetables contain or- ganic acids, generally combined with the 10 THE ATMOSPHERE. SOILS. 11 inorganic bases, or metallic oxides; for these metalic oxides exist in every plant, and may be detected in its ashes after incineration. Nitrogen is an element of vegetable albu- men and gluten ; it is a constituent of the acid, and of what are termed the " indiffer- ent substances" of plants, as well as of those peculiar vegetable compounds which possess all the properties of metallic oxides, and are known as " organic bases." Estimated by : ts proportional weight, ni- trogen forms only a very small part of plants ; but it is never entirely absent from any part of them. Even when it does not absolutely enter into the composition of a particular part or organ, it is always to be found in the fluids which pervade it. It follows from the facts thus far detailed, that the development of a plant requires the presence, first, of substances containing carbon and nitrogen, and capable of yield- ing these elements to the growing organism ; secondly, of water and its elements; and lastly, of a soil to furnish the inorganic matters which are likewise essential to ve- getable life. OF THE COMPOSITION OF THE ATMOSPHERE. In the normal state of growth plants can only derive their nourishment from, the atmosphere and the soil. Hence it is of importance to be acquainted with the com- position of these, in order that we may be enabled to judge from which of their con- stituents the nourishment is afforded. The composition of the atmosphere has been examined by many chemists with great care, and the result of their researches have shown, that its principal constituents are always present in the same proportion. These are the two gases, oxygen and nitro- gen, the general properties of which have been already described. One hundred parts, by weight, of atmospheric air contain 23-1 parts of oxygen, and 76-9 parts of nitrogen ; or 100 volumes of air contain nearly 21 volumes of oxygen gas. From the exten- sive range of affinity which this gas pos- sesses, it is obvious, that were it alone to constitute our atmosphere, and left un- checked to exert its powerful effects, all na- ture would be one scene of universal destruc- tion. It is on this account that nitrogen is present in the air in so large proportion. It is peculiarly adapted for this purpose, as it does not possess any disposition to unite with oxy- gen, and exerts no action upon the processes proceeding on the earth. These two gases are intimately mixed, by virtue of a pro- perty which ail gasses possess in common, of diffusing themselves equally through every part of another gas, with which they are placed in contact. Although oxygen and nitrogen form the principal constituents of the atmosphere, yet they are not the only substances found in it. Watery vapour and carbonic acid gas materially modify its properties. The for- mer of these falls upon the earth as rain, and brings with it any soluble matter which it meets in its passage through the air. Carbonic acid gas is discharged in im- mense quantities from the active volcanoes of America, and from many of the mineral springs which abound in various parts of Europe; it is also generated during the combustion and decay of organic matter. It is not, therefore, surprising that it should have been detected in every part of the atmosphere in which its presence has been looked for. Saussure found it even in the air on the summit of Mont Blanc, which is covered with perpetual snow, and where it could not be produced by the immediate agency of vegetable matter. Carbonic acid gas performs a most important part in the process of vegetable nutrition, the considera- tion of which belongs to another part of the work. Carbonic acid, water, and ammonia (a compound of hydrogen and nitrogen) are the final products of the decay of animal and vegetable matter. In an isolated condition, they usually exist in the gaseous form. Hence, on their formation, they must escape into the atmosphere. But ammonia has not hitherto been enumerated among the con- stituents of the air, although, according to our view, it can never be absent. The rea- son of this is, that it exists in extremely mi- nute quantity in the amount of air usually subjected to experiment in chemical analy- sis ; it has consequently escaped detection. But rain which falls through a large extent of air, carries down in solution all that re- mains in suspension in it. Now ammonia always exists in rainwater, and from this fact we must conclude that it is invariably present in the atmosphere. Nor can we be surprised at its presence when we consider that many volcanoes now in activity emit large quantities of it. This subject will, however, be discussed more fully in anothei part of the work. Such are the principal constituents of the atmosphere from which plants derive their nourishment ; for although other matters are supposed to exist in it in minute quantity, yet they do not exercise any influence on vegetation, nor has even their presence been satisfactorily demonstrated. OF SOILS. A soil may be considered a magazine of inorganic matters, which are prepared by the plant to suit the purposes destined for them in its nutrition. The composition and uses of such substances cannot, however, be studied with advantage, until we have considered the manner in which the organic matter is obtained by plants. Some virgin soils, such as those of Ame- rica, contain vegetable matter in large pro- portion; and as these have been found emi- nently adapted for the cultivation of most plants, the organic matter contained in them 12 AGRICULTURAL CHEMISTRY. has naturally been recognised as the cause of their fertility. To this matter, the terra " vegetable mould" or humus has been ap- plied. Indeed, this peculiar substance ap- pears to play such an important part in the phenomena of vegetation,, that vegetable physiologists have been induced to ascribe the fertility of every soil to its presence. It is believed by many to be the principal nu- triment of plants, and is supposed to be ex- tracted by them from the soil in which they grow. It is itself the product of the decay of vegetable matter, and must, therefore, con- tain many of the constituents which are found in plants during life. Its action will, therefore, be examined in considering whence these constituents are derived. CHAPTER II. OF THE ASSIMILATION OF CARBON. COMPOSITION OF HUMUS. THE humus, to which allusion has been made, is described by chemists as a brown substance easily soluble in alkalies, but only slightly so in water, and produced during the decomposition of vegetable matters by the action of acids or alkalies. It has, how- ever, received various names according to the different external characters and chemi- cal properties which it presents. Thus, ulmin, humic acid, coal of humus, and humin, are names applied to modifications of humus. They are obtained by treating peat, woody fibre, soot, or brown coal with alkalies ; by decomposing sugar, starch, or sugar of milk by means of acids; or by exposing alkaline solutions of tannic and gallic acids to the action of the air. The modifications of humus which are soluble in alkalies, are called humic acid; while those which are insoluble have re- ceived the designations of humin and coal of humas. The names given to these substances might cause it to be supposed that their composition is identical. But a more erro- neous notion could not be entertainad ; since even sugar, acetic acid, and resin do not differ more widely in the proportions of their constituent elements, than do the various modifications of humus. Humic acid formed by the action of hy- drate of potash upon sawdust contains, ac- cording to the accurate analysis of Peligot, 72 per cent, of carbon, while the humic acid obtained from turf and brown coal contains, according to Sprengel, only 58 per cent.; that prod ced by the action of dilute sul- phuric acid upon sugar, 57 per cent, accord- ing to Malaguti; and that, lastly, which is obtained from sugar or from starch, by means of muriatic acid, according to the analysis of Stein, 64 per cent. All these analyses have been repeated with care and accuracy, and the proportion of carbon in the respective cases has been found to agree with the esti- mates of the different chemists above men- tioned ; so that there is no reason to ascribe the difference in this respect between the varieties of humus to the mere difference in the methods of analysis or degrees of ex- pertness of the operators. Malaguti states, moreover, lhat/mmic acid contains an equal number of equivalents of oxygen and hy- drogen, that is to say, that these elements exist in it in the proportions for forming water; while, according to Sprengel, the oxygen is in excess, and Peligot even esti- mates the quantity of oxygen at 14 equiva- lents, and the hydrogen at only 6, equiva- lents, making the deficiency of hydrogen as great as 8 equivalents. And although Mul- der* has very recently explained many of these conflicting results, by showing that there are several kinds of humus and humic acids essentially distinct in their characters, and fixed in their composition, yet he has afforded no proof that the definite compounds obtained by him really exist, as such, in the soil. On the contrary, they appear to have been formed by the action of the potash and ammonia, which he employed in their pre- paration. It is quite evident, therefore, that chemists have been in the habit of designating all products of the decomposition of organic bodies which had a brown or brownish black colour, by the names of humic acid or humin, according as they were soluble or insoluble in alkalies ; although in their composition and mode of origin, the sub- stances thus confounded might be in no way allied. Not the slightest ground exists for the be- lief that one or other of these artificial pro- ducts of the decomposition of vegetable matters exists in nature in the form and en- dowed with the properties of the vegetable constituents of mould ; there is not the shadow of a proof that one of them exerts any influence on the growth of plants either in the way of nourishment or otherwise. Vegetable physiologists have, without any- apparent reason, imputed the known pro- perties of the humus and humic acids of chemists to that constituent of mould which has received the same name, and in this way have been led to their theoretical no- tions respecting the functions of the latter substance in vegetation. The opinion that the substance called humus is extracted from the soil by the roots of plants, and that the carbon entering into its composition serves in some form or other to nourish their tissues, is considered by many as so firmly established that any new argument in its favour has been deemed unnecessary ; the obvious difference in the growth of plants according to the known abundance or scarcity of humus in the soil, * Bulletin des Scienc. Phys. et Natur. de Neerl. 1840, p. 1102. ABSORPTION OP HUMIS. 13 seemed to afford incontestable proof of its correctness.* Yet, this position, when submitted to a strict examination, is found to be untenable, and it becomes evident from most conclusive proofs that humus in the form in which it exists in the soil, does not yield the smallest nourishment to plants. The adherence to the above incorrect opinion has hitherto rendered it impossible for the true theory of the nutritive process in vegetables to become known, and has thus deprived us of our best guide to a rational practice in agriculture. Any great improve- ment in that most important of all arts is in- conceivable without a deeper and more per- fect acquaintance with the substances which nourish plants, and with the sources whence they are derived ; and no other cause can be discovered to account for the fluctuating and uncertain state of our knowledge on this subject up to the present time, than that modern physiology has not kept pace with the rapid progress of chemistry. In the following inquiry we shall suppose the humus of vegetable physiologists to be really endowed with the properties recog- nised by chemists in the brownish black de- posits which they obtain by precipitating an alkaline decoction of mould or peat by means of acids, and which they name humic acid. Humic acid, when first precipitated, is a flocculent substance, is soluble in 2500 times its weight of water, and combines with alkalies, lime and magnesia, forming compounds of the same degree of solubility. (Sprengel.J Vegetable physiologists agree in the sup- position that by the aid of water humus is rendered capable of being absorbed by the roots of plants. But according to the ob- servation of chemists, humic acid is soluble only when newly precipitated, and becomes completely insoluble when dried in the air, or when exposed in the moist state to the freezing temperature. (Sprengel.) Both the cold of winter and the heat of summer, therefore, are destructive of the solu- bility of humic acid, and at the same time of its capability of being assimilated by plants. So that, if 'it is absorbed by plants, it must be in some altered form. The correctness of these observations is easily demonstrated by treating a portion of good mould with cold water. The fluid re- mains colourless, and is found to have dis- solved less than 100,000 part of its weight of organic matters, and to contain merely the salts which are present in rainwater. Decayed oak wood, likewise, of which humic acid is the principal constituent, was found by Berzeliiis to yield to cold water * This remark applies more to German than to English botanists and physiologists. In Eng- land, the idea that humus, as such, affords nour- ishment to plants is by no means general ; but on the Continent, the views of BerzeTius on this sub- ject have been almost universally adopted. ED. only slight traces of soluble materials ; and I have myself verified this observation on. the decayed wood of beech and fir. These" facts, which show that humic, in its unaltered condition, cannot serve for the nourishment of plants, have not escaped the notice of physiologists j and hence they have assumed that the lime or the different alka- lies found in the ashes of vegetables render soluble the humic acid and fit it for the pro- cess of assimulation. Alkalies and alkaline earths do exist in the different kinds of soil in sufficient quan- tity to form such soluble compounds with the humic acid. Now, let us suppose that humic acid is absorbed by plants in the form of that salt which contains the largest proportion of humic acid, namely, in the form of humate j of lime, and then from the known quantity ! of the alkaline bases contained in the ashes | of plants, let us calculate the amount ot humic acid which might be assimulated in this manner. Let us admit, likewise, that potash, soda, and the oxides of iron and ! manganese have the same capacity of satu- ration as lime with respect to humic acid, and then we may take as the basis of our calculation the analysis of M. Berthier, who found that 1000 Ibs. of dry fir wood yielded 4 Ibs. of ashes, and tLat in every 100 Ibs. of i these ashes, after the chloride of potassium and sulphate of potash were extracted, 53 i Ibs. consisted of the basic metallic oxides, potash, soda, lime, magnesia, iron, and manganese. One Hessian acre* of woodland yields j annually, according to Dr. Heyer, on an ' average, 2920 Ibs. of dry fir wood, which contain 6.17 Ibs. of metallic oxides. Now, according to the estimates of Mala- guti and Sprengel, 1 Ib. of lime combines , chemically with 12 Ibs. of humic acid ; 6.17 ; Ibs. of the metallic oxides would accordingly 1 introduce into the trees 67 Ibs. of humic ; acid, which, admitting humic acid to con- ! tain 58 per cent, of carbon, would corres- pond to 100 Ibs. of dry wood. But we have j seen that 2920 Ibs. of fir wood are really produced. Again, if the quantity of humic acid which might be introduced into wheat in the form of humates is calculated from the , known proportion of metallic oxides exist- ing in wheat straw, (the sulphates and chlorides also contained in the ashes of the straw not being included, it will be found that the wheat growing on 1 Hessian acre would receive in that way 63 Ibs. of humic acid, corresponding to 93.6 Ibs. of woody fibre. But the extent of land just mentioned produces, independently of the roots and grain, 1961 Ibs. of straw, the composition of which is the same as that of woody fibre. It has been taken for granted in these cal- * One Hessian acre is equal to 40,000 square feet, Hessian, or 26,910 square feet, English mea- sure. 14 AGRICULTURAL CHEMISTRY. culations that the basic metallic oxides which have served to introduce humic acid into the plants do not return to the soil, since it is certain that they remain fixed in the parts newly formed during the process of growth. Let us now calculate the quantity of humic acid which plants can receive under the most favourable circumstances, viz. the agency of rainwater. The quantity of rain which falls at Er- furt, one of the most fertile districts of Ger- many, during the months of April, May, June, and July, is stated by Schubler to be 19.3 Ibs. over every square foot of surface; 1 Hessian acre, or 26,910 square feet, con- sequently receive 771,000 Ibs. of rainwater. If, now, we suppose that the whole quan- tity of this rain is taken up by the roots of a summer plant, which ripens four months after it is planted, so that not a pound of this water evaporates except from the leaves of the plant ; and if we farther assume that the water thus absorbed is saturated with humate of lime (the most soluble of the hu- mates, and that which contains the largest proportion of humic acid ;) then the plants thus nourished would not receive more than 330 Ibs. of humic acid, since one part of humate of lime requires 2500 parts of water for solution. But the extent of land which we have mentioned produces 2843 Ibs. of corn (in grain and straw, the roots not included,) or 22,000 Ibs. of beet root (without the leaves and small radicle fibres.) It is quite evident that the 330 Ibs. of humic acid, supposed to be absorbed, cannot account for the quantity of carbon contained in the roots and leaves alone, even if the supposition were correct, that the whole of the rainwater was ab- sorbed by the plants. But since it is known that only a small portion of the rainwater which falls upon the surface of the earth evaporates through plants, the quantity of carbon which can be conveyed into them in any conceivable manner by means of humic acid must be extremely trifling, in compa- rison with that actually produced in vege- tation. Other considerations of a higher nature confute the common view respecting the nutritive office of humic acid, in a manner so clear and conclusive that it is difficult to conceive how it could have been so gene- rally adopted. Fertile land produces carbon in the form of wood, hay, grain, and other kinds of growth, the masses of which differ in a re- markable degree. 2920 Ibs. of firs, pines, beeches, &c. grow as wood upon one Hessian acre of forest land with an average soil. The same super- fices yields 2755 Ibs. of hay. A similar surface of corn land gives from 19,000 to 22,000 Ibs. of beet root, or 881 Ibs. of rye, and 1961 Ibs. of straw, 160 sheaves of 15.4 Ibs. each, in all, 2843 Ibs. One hundred parts of dry fir wood con- tain 38 parts of carbon ; therefore, 2920 Ibs. contain 1109 Ibs. of carbon. One hundred parts of hay,* dried in air, contain 44.31 parts carbon. Accordingly, 2755 Ibs. of hay contain 1111 Ibs. of carbon. Beet roots contain from 89 to 89.5 parts water, and from 10.5 to 11 parts solid mat- ter, which consists of from 8 to 9 per cent, sugar, and from 2 to 2 per cent, cellular tissue. Sugar contains 42.4 per cent ; cel- lular tissue, 47 per cent, of carbon. 22,000 Ibs. of beet root, therefore, if they contain 9 per cent, of sugar, and 2 per cent, of cellular tissue, would yield 1032 Ibs. of carbon., of which 833 Ibs. would be due 10 the sugar, and 198 Ibs. to the cellular tissue; the carbon of the leaves and small roots not being included in the calculation. One hundred parts of straw,f dried in air contain 38 per cent, of carbon ; therefore, 1961 Ibs. of straw contain 745 Ibs. of carbon. One hundred parts of corn contain 43 parts of carbon ; 882 Ibs. must, therefore, contain 379 Ibs. in all, 1124 Ibs. of carbon. 26,910 square feet of wood and meadow land produce, consepuently, 1109 Ibs. of carbon ; while the same extent of arable land yields in beet root, without leaves, 1032 Ibs., or in corn, 1124 Ibs. It must be concluded from these incon- testable facts, that equal surfaces of culti- vated land of an average fertility produce equal quantities of carbon; yet, how unlike have been the different conditions of the growth of the plants from which this has been deduced! Let us now inquire whence the grass in a meadow, or the wood in a forest, receives its carbon, since there no manure no car- bon has been given to it as nourishment? and how it happens, that the soil, thus ex- hausted, instead of becoming poorer, be- comes every year richer in this element? A certain quantity of carbon is taken every year from the forest or meadow, in the form of wood or hay, and, in spite of this, the quantity of carbon in the soil aug- ments ; it becomes richer in humus. It is said that in fields and orchards all the carbon which may have been taken away as herbs, as straw, as seeds, or as fruit, is replaced by means of manure ; and yet this soil produces no more carbon than that of the forest or meadow, where it is never replaced. It cannot be conceived that the laws for the nutrition of plants are changed by culture, that the sources of * 100 parts of hay , dried at 100 C. (212 F.) and burned with oxide of copper in a stream of oxygen fas, yielded 51.93 water, 165.8 carbonic acid, and .82 of ashes. This gives 45 87 carbon, 5.76 hy- drogen, 31.55 oxygen, and 6.82 ashes. Hay, dried in the air, loses 11.2 p. c. water at 100 C. (212 F.) (Dr. Will.) t Straw analyzed in the same manner, and dried at 100 C., gave 46.37 p. c. of carbon, 5.68 p. c. of hydrogen, 43.93 p. c. of oxygen, and 4.02 p. c. of ashes. Straw dried in the air at 100 C. lost 18 p. c. of water. Dr. Will. OXYGEN AND CARBON. carbon for fruit or grain, and for grass or trees, are different. It is not denied that manure exercises an influence upon the development of plants ; but it may be affirmed with positive cer- tainty, that it neither serves for the produc- tion of the carbon, nor has any influence upon it, because we find that the quantity of carbon produced by manured lands is not greater than that yielded by lands which are not manured. The discussion as to the manner in which manure acts has nothing to do with the present question, which is, the origin of the carbon. The carbon must be derived from other sources ; and as the Boil does not yield it, it can only be ex- tracted from the atmosphere. In attempting to explain the origin of carbon in plants, it has never been con- sidered that the question is intimately con- nected with that of the origin of humus. It is universally admitted that humas arises from the decay of plants. No primitive humus, therefore, can have existed for plants must have preceded the humus. Now, whence did the first vegetables de- rive their carbon ? and in what form is the carbon contained in the atmosphere ? These two questions involve the conside- ration of two most remarkable natural phe- nomena, which by their reciprocal and un- interrupted influence maintain the life of the individual animals and vegetables, and the continued existence of both kingdoms of or- ganic nature. One of these questions is connected with the invariable condition of the air with re- spect to oxygen. One hundred volumes of air have been found, at every period and in every climate, to contain 21 volumes of oxygen, with such small deviations that they must be ascribed to errors of observation. Although the absolute quantity of oxygen contained in the atmosphere appears very great when represented by numbers, yet it is not inexhaustible. One man consumes by respiration 25 cubic feet of oxygen in 22 hours; 10 cwt. of charcoal consume 32,066 cubic feet of oxygen during its com- bustion; and a small town, like Giessen, f with about 7000 inhabitants) extracts yearly from the air, by the wood employed as fuel, more than 551 millions of cubic feet of this gas. When we consider facts such as these, our former statement, that the quantity of oxygen in the atmosphere does not diminish in the course of ages* that the air at the present day, for example, does not contain less oxygen than that found in jars buried * If the atmosphere possessed, in its whole ex- tent, the same density as it does on the surface of the sea, it would have a height of 24,555 Parisian feet ; but it contains the vapour of water, BO that we may assume its height to be one geo- graphical mile =22,843 Parisian feet. Now the radius of the earth is equal to 860 geographical miles ; hence the for 1800 years in Pompeii appears quite incomprehensible, unless some source exists whence the oxygen abstracted is replaced. How does it happen, then, that the propor- tion of oxygen in the atmosphere is thus invariable ? The answer to this question depends upon another; namely, what becomes of the car- bonic acid, which is produced during the respiration of animals, and by the process of combustion? A cubic foot of oxygen gas, by uniting witn carbon so as to form carbonic acid, does not change its volume. The billions of cubic feet of oxygen ex- tracted from the atmosphere, produce the same number of billions of cubic feet of carbonic acid, which immediately supply its place. The most exact and most recent experi- ments of De Saussure, made in every sea- son for a space of three years, have shown, that the air contains on an average 0'000415 of its own volume of carbonic acid gas ; so that, allowing for the inaccuracies of the experiments, which must diminish the quantity obtained, the proportion of carbonic acid in the atmosphere may be regarded as nearly equal to 1-1000 part of its weight. The quantity varies according to the sea sons ; but the yearly average remains con- tinually the same. We have no reason to believe that this proportion was less in past ages ; and never- theless, the immense masses of carbonic acid which annually flow into the atmos- phere from so many causes, ought percepti- bly to increase its quantity from year to year. But we find that all earlier observers describe its volume as from one-half to ten times greater than that which it has at the present time ; so that we can hence at most conclude that it has diminished. It is quite evident that the quantities of carbonic acid and oxygen in the atmosphere, which remain unchanged by lapse of time, must stand in some fixed relation to one another; a cause must exist which prevents the increase of carbonic acid by removing that which is constantly forming; and there Volume of atmosphere =9,307,500 cubic miles. = cube of 210'4 miles. Volume of oxygen =1,954,578 cubic miles. = cube of 125 miles- Vol. of carbonic acid =3,862'7 cubic miles. = cube of 15'7 miles. The maximum of the carbonic acid contained in the atmosphere has not here been adopted, but the mean, which is equal to 0'000415. A man daily consumes 45,000 cubic inches (Parisian.) A man yearly consumes 9505'2 cubic feet. 100 million men yearly consume 9,505,- 200,000,000 cubic feet. Hence a thousand million men yearly consume G'79745 cubic miles of oxygen. But the air is rendered incapable of supporting the process of respiration, when the quantity of its oxygen is decreased 12 per cent. ; so that a thousand million men would make the air unfit for respiration in a million years. The consumption of oxygen by animals, and by the process of combustion 3 is not introduced into the calculation. 16 AGRICULTURAL CHEMISTRY. must be some means of replacing the oxy- gen, which is removed from the air by the processes of combustion and putre- faction, as well as by the respiration of anmials. Both these causes are united in the pro- cess of vegetable life. The facts which we have stated in the preceding pages prove that the carbon of plants must be derived exclusively from the atmosphere. Now, carbon exists in the atmosphere only in the form of carbonic acid, and therefore, in a state of combination with oxygen. It has been already mentioned likewise, that carbon and the elements of water form the principal constituents of vegetables; the quantity of the substances which do not possess this composition being in a very small proportion. Now, the relative quan- tity of oxygen in the whole mass is less than in carbonic acid ; for the latter contains two equivalents of oxygen, while one only is required to unite with hydrogen in the pro- portion to form water. The vegetable pro- ducts which contain oxygen in larger pro- portion than this, are, comparatively, few in number; indeed, in many the hydrogen is in great excess. It is obvious, that when the hydrogen of water is assimilated by a plant, the oxygen in combination with it must be liberated, and will afford a quantity of this element sufficient for the wants of the plant. If this be the case, the oxygen contained in the carbonic acid is quite unnecessary in the process of vegetable nutrition, and it will consequently escape into the atmosphere in a gaseous form. It is, therefore, certain, that plants must possess the power of decom- posing carbonic acid, since they appropriate its carbon for their own use. The forma- tion of their principal component substances must necessarily be attended with the sepa- ration of the carbon of the carbonic acid from the oxygen, which must be returned to the atmosphere, while the carbon enters into combination with water or its elements. The atmosphere must thus receive a volume of oxygen for every volume of carbonic acid which has been decomposed. This remarkable property of plants has been demonstrated in the most certain man- ner, and it is in the power of every person to convince himself of its existence. The leaves and other green parts of a plant ab- sorb carbonic acid, and emit an equal volume of oxygen. They possess this pro- perty quite independently of the plan-t; for if, after being separated from the stem, they are placed in water containing carbonic acid, and exposed in that condition to the sun's light, the carbonic acid is, after a time, found to have disappeared entirely from the water. If the experiment is conducted un- der a glass receiver filled with water, the oxygen emitted from the plant may be col- ;ected and examined. When no more oxy- gen gas is evolved, it is a sign that all the dissolved carbonic acid is decomposed ; but the operation recommences if a new portion of it is added. Plants do not emit gas when placed in water which either is free from carbonic acid, or contains an alkali that protects it from assimilation. These observations were first made by Priestly and Sennebier. The excellent ex- periments of De Saussure have farther shown, that plants increase in weight dur- ing the decomposition of carbonic acid and separation of oxygen. This increase in weight is greater than can be accounted for by the quantity of carbon assimilated ; a fact which confirms the view, that the elements of water are assimilated at the same time. The life of plants is closely connected with that of animals, in a most simple man- ner, and for a wise and sublime purpose. The presence of a rich and luxuriant vege- tation may be conceived without the con- currence of animal life, but the existence of animals is undoubtedly dependent upon the life and development of plants. Plants not only afford the means of nutri- tion for the growth and continuance of ani- mal organization, but they likewise furnish that which is essential for the support of the important vital process of respiration; for, besides separating all noxious matters from the atmosphere, they are an inexhaustible source of pure oxygen, which supplies the loss which the air is constantly sustaining. Animals on the other hand expire carbon, which plants inspire; and thus the compo- sition of the medium in which both exist, namely, the atmosphere, is maintained con- stantly unchanged. It may be asked is the quantity of car- bonic acid in the atmosphere, which scarcely amounts to 1-1 Oth per cent., sufficient for the wants of the whole vegetation on the surface of the earth, is it possible that the carbon of plants has its origin from the air alone? This question is very easily an- swered. It is known, that a column of air of 2441 Ibs. weight rests upon every square Hessian foot (=0.567 square foot fenglish) of the surface of the earth ; the diameter 01 the earth and its superficies are likewise known, so that the weight of the atmosphere can be calculated with the greatest exactness. The thousandth part of this is caroonic acid, which contains upwards of 27 per cent, car- bon. By this calculation it can be shown, that the atmosphere contains 3306 billion Ibs. of carbon ; a quantity which amounts to more than the weight of all the plants, and of all the strata of mineral and brown coal, which exist upon the earth. This carbon is, therefore, more than adequate to all the purposes for which it is required. The quantity of carbon contained in seawater is proportionally still greater. If, for the sake of argument, we suppose the superficies of the leaves and other green parts of plants, by which the absorption of I carbonic acid is effected, to be double that of the soil upon which they grow, a supposi ASSIMILATION OP CARBON. 17 tion which is much under the truth in the case of woods, meadows, and corn fields ; and if we farther suppose that carbonic acid equal to 0.00067 of the volume of the air, or 1-1 000th of its weight is abstracted from it during every second of time, for eight hours daily, by a field of 53,814 square feet (= 2 Hessian acres ;) then those leaves would receive 1102 Ibs. of carbon in two riundred days.* But it is inconceivable, that the functions of the organs of a plant can cease for any one moment during its life. The roots and other parts of it, which possess the same power, absorb constantly water and carbonic acid. This power is independent of solar light. During the day, when the plants are in the shade, and during the night, carbonic acid is accumulated in all parts of their structure ; and the assimilation of the carbon and the exhalation of oxygen commence from the instant that the rays of the sun strike them. As soon as a young plant breaks through the surface of the ground, it begins to acquire colour from the top downwards j and the true formation of Woody tissue commences at the same time. The proper, constant, and inexhaustible sources of oxygen gas are the tropics and warm climates, where a sky, seldom cloud- ed, permits the glowing rays of the sun to shine upon an immeasurably luxuriant ve- getation. The temperate and cold zones, where artificial warmth must replace defi- cient heat of the sun, produce, on the con- trary, carbonic acid in superabundance, which is expended in the nutrition of the tropical plants. The same stream of air, which moves by the revolution of the earth from the equator to the poles, brings to us in its passage from the equator, the oxygen generated there, and carries away the car- tonic acid formed during our winter. The experiments of De Saussure have * The quantity of carbonic acid which can be ex- tracted from the air in a given time, is shown by the following calculation. During the whitewash- ing of a small chamber, the superficies of the walls and roof of which we will suppose to be 105 square metres, and which receives six coats of lime in four days, carbonic acid is abstracted from the air, and the lime is consequently converted, On the surface, into a carbonate. It has been ac- curately determined that one square decimetre re- ceives in this way, a coating of carbonate of lime which weighs 0.732 grammes. Upon the 105 square metres already mentioned there must ac- cordingly be formed 7686 grammes of carbonate of lime, which contain 4325.6 grammes of carbo- nic acid. The weight of one cubic decimetre of carbonic acid being calculated at two grammes, (more accurately 1.97973.) the above mentioned surface must absorb in four days 2.163 cubic me- tres of carbonic acid. 2500 square metres (one Hessian acre) would absorb, under a similar treat- ment, 51 i cubic metres=1818 cubic feet of car- bonic acid in four days. In 200 days it would ab- sorb 2575 cubic metres=904,401 cubic feet, which contain 11,353 Ibs. of carbonic acid, of which 3304 Ibs. are carbon, a quantity three times as great as that which is assimilated by the leaves and roots growing upon the same space. 3 proved, that the upper strata of the air con- tain more carbonic acid than the lower, which are in contact with plants j and that the quantity is greater by night than by day, when it undergoes decomposition. Plants thus improve the air by the remo- val of carbonic acid, and by the renewal of oxygen, which is immediately applied to the use of man and animals. The horizon- tal currents of the atmosphere bring with them as much as they carry away, and the interchange of air between the upper and lower strata, which their differencp of tem- perature causes, is extremely trilling when compared with the horizontal movements of the winds. Thus vegetable culture heightens the healthy state of a country, and a previously healthy country would be rendered quite uninhabitable by the cessa- tion of all cultivation. The various layers of wood and mineral coal, as well as peat, form the remains of a primeval vegetation. The carbon which they contain must have been originally in the atmosphere as carbonic acid in which form it was assimilated by the plants which constitute these formations. It follows from this, that the atmosphere must be richer in oxygen at the present time than in former periods of the earth's history. The increase must be exactly proportional to the quantity of carbon and hydrogen contained in these carboniferous deposits. Thus, during the formation of 353 cubic feet of Newcastle splint coal, the atmosphere must have re- ceived 643 cubic feet of oxygen produced from the carbonic acid assimilated, and also 158 cubic feet of the same gas resulting from the decomposition of water. In for- mer ages, therefore, the atmosphere must have contained less oxygen, but a much larger proportion of carbonic acid, than it does at the present time, a circumstance which accounts for the richness and luxuri- ance of the earlier vegetation. But a certain period must have arrived in which the quantity of carbonic acid con- tained in the air experienced neither increase nor diminution in any appreciable quantity. For if it received an additional quantity to its usual proportion, an increased vegetation would be the natural consequence, and the excess would thus be speedily removed. And, on the other hand, if the gas was less than the normal quantity, the progress of vegetation would be retarded, and the proportion would soon attain its proper standard. The most important function in the life of plants, or, in other words, in their as- similation of carbon, is the separation, we might almost say the generation, of oxygen, No matter can be considered as nutritious, or as necessary to the growth of plants, which possesses a composition either simi- lar to or identical with theirs, and the as- similation of which, therefore, could take place without exercising this function. The reverse is the case in the nutrition of ani- 18 AGRICULTURAL CHEMISTRY. mals. Hence such substances as sugar, starch, and gum, which are themselves pro- ducts of plants, cannot be adopted for as- similation. And this is rendered certain by the experiments of vegetable physiologists, who have shown that aqueous solutions of these bodies are imbibed by the roots of plants, and carried to all parts of their struc- ture, but are not assimilated, they cannot, therefore, be employed in their nutrition. We could scarcely conceive a form more convenient for assimilation than that of gum, starch, and sugar, for they ah contain the elements of woody fibre, and nearly in the same proportions. In the second part of the work we shall adduce satisfactory proofs that decayed woody fibre (humus) contains carbon and the elements of water, without an excess of oxygen ; its composition differing from that of woody fibre in its being richer in carbon. Misled by this simplicity in its constitu- tion, physiologists found no difficulty in dis- covering the mode of the formation of woody fibre ; for they say,* humus has only to enter into combination with water, in order to effect the formation of woody fibre, and other substances similarly composed, such as sugar, starch, and gum. But they forget that their own experiments have suf- ficiently demonstrated the inaptitude of these substances for assimilation. All the erroneous opinions concerning the modus operandi of humus have their origin in the false notions entertained respecting the most important vital functions of plants ; analogy, that fertile source of error, having, unfortunately, led to the very unapt com- parison of the vital functions of plants with those of animals. Substances, such as sugar, starch, &c., which contain carbon and the elements of water, are products of the life of plants which live only while they generate them. The same may be said of humus, for it can be formed in plants like the former sub- stances. Smithson, Jameson, and Thomson, found that the black excretions of unhealthy elms, oaks, and horse chesnuts, consisted of humic acid in combination with alkalies. Berzelius detected similar products in the bark of most trees. Now, can it be supposed that the diseased organs of a plant possess the power of generating the matter to which its substance and vigour are ascribed? How does it happen, it may be asked, that the absorption of carbon from the atmos- phere by plants is doubted by all botanists and vegetable physiologists, and that by the greater number the purification of the air by means of them is wholly denied ? The action of plants on the air in the absence of light, that is during night, has been much misconceived by botanists, and from this we may trace most of the errors which abound in the greater part of their writings. The experiments of Ingenhouss Meyen, PJlanzenphysiologie, II. S. 141. were in a great degree the cause of this un- certainty of opinion regarding the influence of plants in purifying the air. His obser- vation that green plants emit carbonic acid in the dark, led De Saussure and Grischow to new investigations, by which they ascer- tained that under such conditions plants do really absorb oxygen and emit carbonic acid ; but that the whole volume of air undergoes diminution at the same time. From the latter fact it follows, that the quantity of oxygen gas absorbed is greater than the volume of carbonic acid separated ; for, if this were not the case, no diminution could occur. These facts cannot be doubted, but the views based on them have been so false, that nothing, except the total want of obser- vation and the utmost ignorance of the chemical relations of plants to the atmos- phere, can account for their adoptio^i. It is known that nitrogen, hydrogen, and a number of other gases, exercise a pecu- liar, and in general, an injurious influence upon living plants. Is it, then, probable, that oxygen, one of the most energetic agents in nature, should remain without influence on plants when one of their peculiar processes of assimilation has ceased ? It is true that the decomposition of car- bonic acid is arrested by absence of light. But then, namely, at night, a true chemical process commences, in consequence of the action of the oxygen in the air, upon the organic substances composing the leaves, blossoms, and fruit. This process is not at all connected with the life of the vegetable organism, because it goes on in a dead plant exactly as in a living one. The substances composing the leaves of different plants being known, it is a matter of the greatest ease and certainty to calcu- late which of them, during life, should ab- sorb most oxygen by chemical action when the influence of light is withdrawn. The leaves and green parts of all plants containing volatile oils or volatile constitu- ents in general, which change into resin by the absorption of oxygen, should absorb more than other parts which are free from such substances. Those leaves, also, which contain either the constituents of nutgalls, or compounds in which nitrogen is present, ought to absorb more oxygen than those which do not contain such matters. The correctness of these inferences has been dis- tinctly proved by the observations of De Saussure ; for, while the tasteless leaves of the Jlgave americana absorb only 0-3 of their volume of oxygen in the dark, during 24 hours, the leaves of the Pinus Mies,, which contain volatile and resinous oils, absorb 10 times, those of the Quercus Robwr containing tannic acid 14 times, and the balmy leaves of the Populus alba 21 times that quantity. This chemical action is shown very plainly also, in the leaves of the Cotyledon calycinum, the Cacaliajicoides, and others j for they are sour like sorrel in the morning, tasteless at noon, and bitter in ASSIMILATION OF CARBON. 19 the evening. The formation of acids is effected during the night by a true process of oxidation: these are deprived of their acid properties during the day and evening, and are changed by separation of a part of their oxygen into compounds containing oxygen and hydrogen, either in the same proportions as in water, or even with an excess of hydrogen, which is the composi- tion of all tasteless and bitter substances. Indeed, the quantity of oxygen absorbed could be estimated pretty nearly by the dif- ferent periods which the green leaves of plants require to undergo alteration in colour, by the influence of the atmosphere. Those which continue longest green will abstract less oxygen from the air iu an equal space of time, than those the constituent parts of which suffer a more rapid change. It is found, for example, that the leaves of the Ilex aquifolium, distinguished by the dura- bility of their colour, absorb only O86 of their volume of oxygen gas in the same time that the leaves of the poplar absorb 8, and those of the beech 9$ times their volume ; both the beech and poplar being remarkable for the rapidity and ease with which the colour of their leaves changes. When the green leaves of the poplar, the beech, the oak, or the holly, are dried under the air pump, with exclusion of light, then moistened with water, and placed under a glass globe filled with oxygen, they are found to absorb that gas in proportion as they change in colour. The chemical nature of this process is thus completely established. The diminution of the gas which occurs can only be owing to the union of a large pro- j portion of oxygen with those substances j which are already in the state of oxides, or | to the oxidation of the hydrogen in those | vegetable compounds which contain it in excess. The fallen brown or yellow leaves of the oak contain no longer tannin, and those of the poplar no balsamic constituents. The property which green leaves possess of absorbing oxygen belongs also to fresh wood, whether taken from a twig or from the interior of the trunk of a tree. When fine chips of such wood are placed in a moist condition under a jar filled with oxy- gen, the gas is seen to diminish in volume. But wood, dried by exposure to the atmo- sphere and then moistened, converts the oxygen into carbonic acid, without change of volume ; fresh wood^ therefore, absorbs most oxygen. MM. Petersen and Schodler have shown, by the careful elementary analysis of 24 dif- ferent kinds of wood, that they contain car- bon and the elements of water, with the addition of a certain quantity of hydrogen. Oak wood, recently taken from the tree, and dried at 10(P C. (212 F.,) contains 49,432 carbon, 6.069 hydrogen, and 44.499 oxygen. The proportion of hydrogen which is ne- cessary to combine with 44.498 oxygen in order to form water, is - of this quantity, namely, 5.56 j it is evident, therefore, that oak wood contains ^ more hydrogen than corresponds to this proportion. In Pinus Larix, P. Mies, and P. picea, the excess of hydrogen amounts to \, and in Tilia euro- p&a to i. The quantity of hydrogen stands in some relation to the specific weight of the wood; the lighter kinds of wood contain more of it than the heavier. In ebony wood (Diospyros Ebenuni) the oxygen and hydro- gen are in exactly the same proportion as in water. The difference between the composition of the varieties of wood, and that of simple woody fibre, depends, unquestionably, upon the presence of constituents, in part soluble, and in part insoluble, such as resin and other matters, which contain a large pro- portion of hydrogen : the hydrogen of such substances being in the analysis of the vari- ous woods superadded to that of the true woody fibre. It has previously been mentioned that mouldering oak wood contains carbon and the elements of water, without any excess of hydrogen. But the proportions of its constituents must necessarily have been dif- ferent, if the volume of the air had not changed during its decay, because the pro- portion of hydrogen in those component substances of the wood which contained it in excess is here diminished, and this dimi- nution could only be effected by an absorp- tion of oxygen, and consequent formation of water. Most vegetable physiologists have con- nected the emission of carbonic acid during the night with the absorption of oxygen from the atmosphere, and have considered these actions as a true process of respiration in plants, similar to that of animals, and like it, having for its result the separation of carbon from some of their constituents. This opinion has a very weak and unstable foundation. The carbonic acid, which has been ab- sorbed by the leaves and by the roots, to- gether with water, ceases to be decomposed on the departure of daylight ; it is dissolved in the juices which pervade all parts of the plant, and escapes every moment through the leaves in quantity corresponding to that of the water which evaporates. A soil in which plants vegetate vigor- ously, contains a certain quantity of mois- ture which is indispensably necessary to their existence. Carbonic acid, likewise, is always present in such a soil, whether it has been abstracted from the air or has been generated by the decay of vegetable matter. Rain and wellwater, and also that from other sources, invariably contains carbonic acid. Plants during their life constantly possess the power of absorbing by their roots moisture, and, along with it, air and carbonic acid. Is it, therefore, surprising that the carbonic acid should be returned unchanged to the atmosphere, along with water, when light (the cause of the fixation of its carbon) is absent ? 20 AGRICULTURAL CHEMISTRY. Neither this emission of carbonic acid nor the absorption of oxygen has any connection with the process of assimilation; nor have they the slightest relation to one another; the one is a purely mechanical,, the other a purely chemical process. A cotton wick, inclosed in a lamp, which contains a liquid saturated with carbonic acid, acts exactly in the same manner as a living plant in the night. Water and carbonic acid are sucked up by capillary attraction, and both evapo- rate from the exterior part of the wick. Plants which live in a soil containing hu- mus exhale much more carbonic acid dur- ing the night. than those which grow in dry situations ; they also yield more in rainy than in dry weather. These facts point out to us the cause of the numerous contradic- tory observations, which have been made with respect to the change impressed upon the air by living plants, both in darkness and in common daylight, but which are un- worthy of consideration, as they do not assist in the solution of the main question. There are other facts Avhich prove in a de- cisive manner that plants yield more oxygen to the atmosphere than they extract from it; these proofs, however, are to be drawn with certainly only from plants which live under water. When pools and ditches, the bottoms of which are covered with growing plants, freeze upon their surface in winter, so that the water is completely excluded from the atmosphere by a clear stratum of ice, small bubbles of gas are observed to escape, con- tinually, during the day, from the points of the leaves and twigs. These bubbles are seen most distinctly when the rays of the sun fall upon the ice ; they are very small at first, but collect under the ice and form larger bubbles. They consist of pure oxy- gen gas. Neither during the night, nor dur- ing the day when the sun does not shine, are they observed to diminish in quantity. The source of this oxygen is the carbonic acid dissolved in the water, which is ab- sorbed by the plants, but is again supplied to the water, by the decay of vegetable sub- stances contained in the soil: If these plants absorb oxygen during the night, it can be in no greater quantity than that which the sur- rounding water holds in solution, for the gas, which has been exhaled, is not again absorbed. The action of water plants can- not be supposed to form an exception to a great law of nature, and the less so, as the different action of aerial plants upon the at- mosphere is very easily explained. The opinion is not new that the carbonic acid of the air serves for the nutriment of plants, and that its carbon is assimilated by them ; it has been admitted, defended, and argued for, by the soundest and most intelli- gent natural philosophers, namely, by Priest- ley, Sennebier, De Saussure, and even by Ingenhouss himself. There scarcely exists a theory in natural science, in favour of which there are more clear and decisive ar- guments. How, then, are we to account for its not being received in its full extent by most other physiologists, for its being even disputed by many, and considered by a few as quite refuted ? All this is due to two causes, which we shall now consider. One is, that in botany the talent and la- bour of inquirers has been wholly spent in the examination of form and structure : che- mistry and physics have not been allowed to sit in council upon the explanation of the most simple processes ; their experience and their laws have not been employed, though the most powerful means of help in the ac- quirement of true knowledge. They have not been used, because their study has been neglected. All discoveries in physics and in chemis- try, all explanations of chemists, must re- main without fruit and useless, because, even to the great leaders in physiology, car- bonic acid, ammonia, acids, and bases, are sounds without meaning, words without sense, terms of an unknown language, which awaken no thoughts and no associations. They treat these sciences like the vulgar, who despise a foreign literature in exact proportion to their ignorance of it ; since even when they have had some acquintance with them, they have not understood their spirit and application. Physiologists reject the aid of chemistry in their inquiry into the secrets of vitality, although it alone could guide them in the true path ; they reject chemistry, because in its pursuit of knowledge it destroys the sub- jects of its investigation ; but they forget that the knife of the anatomist must dis- member the body, and destroy its organs, if an account is to be given of their form, structure, and functions. When pure potato starch is dissolved in nitric acid, a ring of the finest wax remains. What can be opposed to the conclusion of the chemist, that each grain of starch con- sists of concentric layers of wax and amylin, which thus mutually protect each other against the action of water and ether ? Can results of this kind, which illustrate so com- pletely both the nature and properties of bodies, be attained by the microscope ? Is it possible to make the gluten in a piece of bread visible in all its connections and rami- fications ? It is impossible by means of in- struments ; but if the piece of bread is placed in a lukewarm decoction of malt, the starch, and the substance called dextrine,* are seen to dissolve like sugar in water, and, at last, nothing remains except the gluten, in the * According to Raspail, starch consists of vesi- cles inclosing within them a fluid resembling gum. Starch may be put in cold water without being dissolved : but, when placed in hot water, these spherules burst, and allow the escape of the liquid. This liquid is the dextrine of Biot, so called be- cause it possesses the property of turning the plane of the polarization of light to the right hand. ED. ASSIMILATION OF CARBON. 21 form of a spongy mass, the minute pores of which can be seen only by a microscope. Chemistry offers innumerable resources of this kind which are of the greatest use in an inquiry into the nature of the organs of plants; but they are not used, because the need of them is not felt. The most import- ant organs of animals and their functions are known, although they may not he visi- ble to the naked eye. But in vegetable phy- siology, a leaf is in every case regarded merely as a leaf, notwithstanding that leaves generating oil of turpentine or oil of lemons must possess a different nature from those in which oxalic acid is formed. Vitality, in its peculiar operations, makes use of a spe- cial apparatus for each function of an organ. A rose twig engrafted upon a lemon tree does not bring forth lemons, but roses. Vegetable physiologists in the study of their science have not directed their attention to that part of it which is most worthy of in- vestigation. The second cause of the incredulity with which physiologists view the theory of the nutrition of plants by the carbonic acid of the atmosphere is, that the art of experi- menting is not known in physiology, it being an art which can be learned accurately only in the chemical laboratory. Nature speaks to us in a peculiar language, in the language of phenomena; she answers at all times the questions which are put to her; and such questions are experiments. An experiment is the expression of a thought: we are near the truth when the phenomena elicited by the experiment corresponds to the thought; while the opposite result shows that the question was falsely stated, and that the conception was erroneous. The critical repetition of another's experi- ments must be viewed as a criticism of his opinions; if the result of the criticism be merely negative, if it do not suggest more correct ideas in the place of those which it is intended to refute, it should be disre- garded ; because the worse experimenter the critic is, the greater will be the discrepancy between the results he obtains and the views proposed by the other. It is too much forgotten by physiologists, that their duty really is not to refute the ex- periments of others, nor to show that they are erroneous, but to discover truth, and that alone. It is startling, when we reflect that all the time and energy of a multitude of persons of genius, talent, and knowledge, are expended in endeavours to demonstrate each other's errors. The question whether carbonic acid is the food of plants or not has been made the sub- ject of experiments with perfect zeal and good faith; th3 results have been opposed to that view. But how was tne inquiry in- stituted ? The seeds of balsamines, beans, cresses, and gourds, were sown in pure Carrara marble, and sprinkled with water containing carbonic acid. The seeds sprang, but the plants did not attain to the development of the third small leaf. In other cases, they allowed the water to penetrate the marble from below, yet, in spite of this, they died. It is worthy of observation, that they lived longer with pure distilled water than with that impregnated with carbonic acid; but still, in this case also, they eventually pe- rished. Other experimenters sowed seeds of plants in flowers of sulphur and sulphate of barytes, and tried to nourish them with carbonic acid, but without success. Such experiments have been considered as positive proofs, that carbonic acid will not nourish plants ; but the manner in which they were instituted is opposed to all rules of philosophical inquiry, and to all the laws of chemistry. Many conditions are necessary for the life of plants ; those of each genus require special conditions ; and should but one of these be wanting, although the rest be sup- plied, the plants" will not be brought to ma- turity. The organs of a plant, as well as those of an animal, contain substances of the most different kinds ; some are formed solely of carbon and the elements of water, others contain nitrogen, and in all plants we find metallic oxides in the state of salts. The food which can serve for the produc- tion of all the organs of a plant, must neces- sarily contain all its elements. These most essential of all the chemical qualities of nu- triment may be united in one substance, or they may exist separately in several ; in which case, the one contains what is want- ing in the other. Dogs die although fed with jelly, a substance which contains ni- trogen ; they cannot live upon white bread, sugar or starch, if these are given as food, to the exclusion of all other substances. Can it be concluded from this, that these substances contain no elements suited for assimilation? Certainly not. Vitality is the power which each organ possesses of constantly reproducing itself; for this it requires a supply of substances which contain the constituent elements of its own substance, and are capable of undergoing transformation. All the organs together cannot generate a single element, carbon, nitrogen, or a metallic oxide. When the quantity of the food is too great, or is not capable of undergoing the necessary transformation, or exerts any pe- culiar chemical action, the organ itself is subjected to a change : all poisons act in this manner. The most nutritious substances may cause death. In experiments such as those described above, every condition of nutrition should be considered. Besides those matters whv*-h form their principal constituent parts, botn animals and plants require others, the peculiar functions ot which are unknown. These are inorganic substances, such as common salt, the total want of which is in animals inevitably pro- ductive of death. Plants, for tne same rea- AGRICULTURAL CHEMISTRY. son, cannot live unless supplied with cer- tain metallic compounds. If we knew with certainty that there ex- isted a substance capable alone of nour- ishing a plant and of bringing it to maturity, we might be led to a knowledge of the con- ditions necessary to the life of all plants, by. studying its characters and composition. If humus were such a substance, it would have precisely the same value as the only single food which nature has produced for animal organization, namely, milk (Prout.) The constituents of milk are cheese or caseine, a compound containing nitrogen in large proportion ; butter, in which hydrogen abounds; and sugar of milk, a substance with a large quantity of hydrogen and oxy- gen in the same proportion as in water. It also contains in solution, lactate of soda, phosphate of lime, and common salt ; and a peculiar aromatic product exists in the but- ter, called butyric acid. The knowledge of the composition of milk is a key to the con- ditions necessary for the purposes of nutri- tion of all animals. All substances which are adequate to the nourishment of animals contain those ma- terials united, though not always in the same form ; nor can any one be wanting for a certain space of time, without a marked effect on the health being produced. The employment of a substance as food presup- poses a knowledge of its capacity of assimi- lation, and of the conditions under which this takes place. A carnivorous animal dies in the vacuum of an air pump, even though supplied with a superabundance of food ; it dies in the air, if the demands of its stomach are not satis- fied ; and it dies in pure oxygen gas, how- ever lavishly nourishment be given to it. Is it hence to be concluded, that neither flesh, nor air, nor oxygen, is fitted to support life ? Certainly not. From the pedestal of the Trajan column at Rome we might chisel out each single piece of stone, if upon the extraction of the second we replaced the first. But could we conclude from this that the column was sus- pended in the air, and not supported by a single piece of its foundation ? Assuredly not. Yet the strongest proof would have been given that each portion of the pedestal could be removed, without the downfall of the column. Animal and vegetable physiologists, how- ever, come to such conclusions with re- spect to the process of assimilation. They institute experiments, without being ac- quainted with the circumstances necessary for the continuance of life with the quali- ties and proper nutriment of the animal or plant on which they operate or with the nature and chemical constitution of its organs. These experiments are considered by them as convincing proofs, while they are fitted only to awaken pity. Is it possible to bring a plant "to maturity by means of carbonic acid and water, with- out the aid of some substance containing ni- trogen, which is an essential constituent of the sap, and indispensable for its produc- tion ? Must the plant not die, however abundant the supply of carbonic acid may be, as soon as the first small leaves have exhausted the nitrogen contained in the seeds ? Can a plant be expected to grow in Car- rara marble, even when an azotised sub- stance is supplied to it, if the marble be sprinkled with an aqueous solution of car- bonic acid, which dissolves the lime and forms bicarbonate of lime? A plant of the family of the Plumbagineoe, upon the leaves of which fine hornlike, or scaly processes of crystallised carbonate of lime are formed, might, perhaps, attain maturity under such circumstances; but these experiments are only sufficient to prove, that cresses, gourds, and balsamines, cannot be nourished by bicarbonate of lime, in the absence of mat- ter containing nitrogen. We may, indeed, conclude, that the salt of lime acts as a poison, since the developement of plants will advance farther in pure water, when lime and carbonic acid are not used. Moist flowers of sulphur attract oxygen from the atmosphere, and become acid. Is it possible that a plant can grow and flourish in presence of free sulphuric acid, with no other nourishment than carbonic acid ? It is true, the quantity of sulphuric acid formed thus in hours, or in days, may be small, but the property of each particle of the sulphur to absorb oxygen and retain it, is present every moment. When it is known that pants require moisture, carbonic acid, and air, should we choose as the soil for experiments on their growth, sulphate of barytes, which, from its nature and specific gravity, completely pre- vents the access of air? All these experiments are valueless for the decision of any question. It is absurd to take for them any soil, at mere hazard, as long as we are ignorant of the functions performed in plants by those inorganic sub- stances which are apparently foreign to them. It is quite impossible to mature a plant of the family of the Graminece, or of the Equisctacece, the solid framework of which contains silicate of potash, without silicic acid and potash, or a plant of the ge- nus Oxalis without potash, or saline plants such as the saltworts (Salsola and Scdicornia) without chloride of sodium, or at least some salt of similar properties. All seeds of the Graminece contain phosphate of magnesia; the solid parts of the roots of the althcea con- tain more phosphate of lime than woody fibre. Are these substances merely accidentally present ? A plant should not be chosen for experiment, when the matter which it re- quires for its assimilation is not well known. What value, now, can be attached to ex- periments in which all those matters which a plant requires in the process of assimila- tion, besides its mere nutriment, 1 ave been ORIGIN AND ACTION OF HUMUS. 23 excluded with the greatest care ? Can the laws of life be investigated in an organised being which is diseased or dying? The mere observation of a wood or mea- dow is infinitely better adapted to decide so simple a question than all the trivial experi- ments under a glass globe; the only dif- ference is that instead of one plant there are thousands. When we are acquainted with the nature of a single cubic inch of their scil, and know the composition of the air and rainwater, we are in possession of ah 1 the conditions necessary to their life. The source of the different elements entering into the composition of plants cannot possibly escape us, if we know in what form they take up their nourishment, and compare its composition with that of the vegetable sub- stances which compose their structure. All these questions will now be examined and discussed. It has been already shown that the carbon of plants is derived from the atmosphere : it still remains for us to in- quire what power is exerted on vegetation by the humus of the soil and the inorganic constituents of plants and also to trace the sources of their nitrogen. CHAPTER III. ON THE ORIGIN AND ACTION OF HUMUS. IT will be shown in the second part of this work, that all plants and vegetable structures undergo two processes of decom- position after death. One of these is named fermentation; the other, putrefaction, decay, or ercmacausis* It will likewise be shown, that decay is a slow process of combustion, a process, therefore, in which the combustible parts of a plant unite with the oxygen of the atmo- sphere. The decay of woody fibre (the principal constituent of all plants) is accompanied by a phenomenon of a peculiar kind. This substance, in contact with air or oxygen gas, converts the latter into an equal volume of carbonic acid, and its decay ceases upon the disappearance of the oxygen. If the carbonic acid is removed, and oxygen re- placed, its decay recommences, that is, it again converts oxygen into carbonic acid. Woody fibre consists of carbon and the ele- ments of water ; and if we judge only from the products formed during its decomposi- tion, and from those formed by pure char- coal, burned at a high temperature, we might conclude that the causes were the same in both : the decay of woody fibre pro- ceeds, therefore, as if no hydrogen or oxy- gen entered into its composition. * The word eremacausis was proposed by the author some time since, in order to explain the true nature of decay ; it is compounded from itfp*, by degrees and *?*/?, burning. A very long time is required for the com- pletion of this process of combustion, and the presence of water is necessary for its main- tenance : alkalies promote it, but acids re- tard it; all antiseptic substances, such as sulphurous acid, the mercurial salts, empy- reumatic oils, Sec., cause its complete ces- sation. Woody fibre in a state of decay is the substance called humus.* The property of woody fibre f ? ov nvert surrounding oxygen gas into carbohx: acid diminishes in proportion as its decay ad- vances, and at last a certain quantity of a brown coaly-looking substance remains, in which this property is entirely wanting. This substance is called mould; it is the product of the complete decay of woody fibre. Mould constitutes the principal of ail the strata of brown coal and peat. Humus acts in the same manner in a soil permeable to air as in the air itself; it is a continued source of carbonic acid, which it emits very slowly. An atmosphere of car- bonic acid, formed at the expense of the air, surrounds every particle of decaying humus. The cultivation of land, by tilling and loos- ening the soil, causes a free and unob- structed access of air. An atmosphere of carbonic acid is, therefore, contained in every fertile soil, and is the first and most import- ant food for the young plants which grow in it. In spring, when those organs of plants are absent which nature has appointed for the assumption of nourishment from the atmosphere, the component substance of the seeds is exclusively employed in the forma- tion of the roots. Each new radicle fibril which a plant acquires may be regarded as constituting at the same time a mouth, a lung, and a stomach. The roots perform the functions of the leaves from the first moment of their formation : they extract from the soil their proper nutriment, namely, the carbonic acid generated by the humus. By loosening the soil which surrounds young plants, we favour the access of air, and the formation of carbonic acid; and, on the other hand, the quantity of their food is diminished by every difficulty which op- poses the renewal of air. A plant itself effects this change of air at a certain period of its growth. The carbonic acid, which protects the undecayed humus from farther change, is absorbed and taken away by the fine fibres of the roots, and by the roots themselves ; this is replaced by atmospheric air, by which process the decay is renewed, and a fresh portion of carbonic acid formed. A plant at this time receives its food both by the roots and by the organs above ground, and advances rapidly to maturity. When a plant is quite matured, and when * The humic acid of chemists is a product of the decomposition of humus by alkalies ; it does not exist in the humus of vegetable physiologists- AGRICULTURAL CHEMISTRY. the organs by which it obtains food from the atmosphere are formed, the carbonic acid of the soil is no farther required. Deficiency of moisture in the soil, or its complete dryness, does not now check the growth of a plant, provided it receives from the dew and the atmosphere as much as is requisite for the process of assimilation. During the heat of summer it derives its carbon exclusively from the atmosphere. We do not knoAv what height and strength nature has allotted to plants; we are ac- quainted only with the size which they usually attain. Oaks are shown, both in London and Amsterdam, as remarkable curiosities, which have been reared by Chi- nese gardeners, and are only one foot and a half in height, although their trunks, barks, leaves, branches, and whole habitus, evince a venerable age. The small parsnep grown at Teltow,* when placed in a soil which yields as much nourishment as it can take up, increases to several pounds in weight. The size of a plant is proportional to the surface of the organs which are destined to convey food to it. A plant gains another mouth and stomach with every new fibre of root, and every new leaf. The power which roots possess of taking up nourishment does not cease as long as nutriment is present. When the food of a plant is in greater quantity than its organs require for their own perfect development, the superfluous nutriment is not returned to the soil, but is employed in the formation of new organs. At the side of a cell, already formed, another cell arises ; at the side of a twig and leaf, a new twig and a new leaf are developed. These new parts could not have been formed had there not been an excess of nourishment. The sugar and mucilage produced in the seeds, form the nutriment of the young plants, and disap- pear during the development of the buds, green sprouts, and leaves. The power of absorbing nutriment from the atmosphere, with which the leaves of plants are endowed, being proportionate to the extent of their surface, every increase in the size and number of these parts is ne- cessarily attended with an increase of nutri- tive power, and a consequent farther de- velopment of new leaves and branches. Leaves, twigs, and branches, when com- pletely matured, as they do not become larger, do not need food for their support. For their existence as organs, they require only the means necessary for the perform- ance of the special functions to which they are destined by nature ; they do not exist on their own account. We know that the functions of the leaves and other green parts of plants are to absorb carbonic acid, and with the aid of light and * Teltow is a village near Berlin, where small parsneps are cultivate4 in a sandy soil ; they are .^uch esteemed, and weigh rarely above one cvmce. moisture, to appropriate its carbon. These processes are continually in operation ; they commence with the first formation of the leaves, and do not cease with their perfect- development. But the new products arising from this continued assimilation are no longer employed by the perfect leaves in their own increase : they serve for the for- mation of woody fibre, and all the solid matters of similar composition. The leaves now produce sugar, amylin or starch, and acids, which were previously formed by the roots when they were necessary for the de- velopment of the stem, buds, leaves, and branches of the plant. The organs of assimilation, at this period of their life, receive more nourishment from the atmosphere than they employ in their own sustenance ; and when the formation of the woody substance has advanced to a certain extent, the expenditure of the nutri- ment, the supply of which still remains the same, takes a new direction, and blossoms are produced. The functions of the leaves of most plants cease upon the ripening of their fruit, because the products of their action are no longer needed. They now yield to the chemical influence of the oxygen of the air, generally suffer a change in colour, and fall off. A peculiar " transformation' > of the mat- ters contained in all plants takes place in the period between blossoming and me ripening of the fruit ; new compounds are produced, which furnish constituents of the blossoms, fruit, and seed. An organic chemical "transformation" is the separation of the elements of one or several combinations, and their re-union into two or several others, which contain the same number of elements, either grouped in another manner, or in dif- ferent proportions. Of two compounds formed in consequence of such a change, one remains as a component part of the blossom or fruit, while the other is separated by the roots in the form of excrementitious matter. No process of nutrition can be con- ceived to subsist in animals or vegetables, without a separation of effete matters. We know, indeed, that an organized body can- not generate substances, but can only change the mode of their combination, and that its sustenance and reproduction depend upon the chemical transformation of the matters which are employed as its nutriment, and which contain its own constituent elements. Whatever we regard as the cause of these transformations, whether the Vital Principle, Increase of Temperature, Light, Galvanism, or any other influence, the act of transfor- mation is a purely chemical process. Com- bination and Decomposition can take place only when the elements are disposed to these changes. That which chemists name affinity indicates only the degree in which they possess this disposition. It will be shown, when considering the processes of fermentation and putrefaction, that every disturbance of the mutual attraction sub- ORGANIC SUBSTANCES. sisting between the elements of a body gives rise to a transformation. The elements ar- range themselves according to the degrees of their reciprocal attraction into new com- binations, which are incapable of farther change under the same conditions. The products of these transformations vary with their causes, that is, with the dif- ferent conditions on which their production depended ; and are as innumerable as these conditions themselves. The chemical cha- racter of an acid, for example, is its un- ceasing disposition to saturation by means of a base ; this disposition differs in intensity in different acids ; but when it is satisfied, the acid character entirely disappears. The chemical character of a base is exactly the reverse of this, but both an acid and a base, notwithstanding the great difference in their properties, effect, in most cases, the same kin.1 of transformations. Hvdrocyanic acid and water contain the elements of carbonic acid, ammonia, urea, cyanvric acid, cyanilic acid, oxalic acid, for- mic acid, mi-lam, ammclin, melamin, azulmin, mr.llon. Injihomdlonic acid, allantoin, fyc. It is well known, that all these very different substances can be obtained from hydrocyanic acid and the elements of water, by various chemical transformations. The whole process of nutrition may be understood by the consideration of one of these transformations. Hydrocyanic acid and water, for example, when brought into contact with muriatic acid, are decomposed into formic acid and ammonia ; both of these products of decom- position contain the elements of hydrocyanic acid and water, although in another form, and arranged in a different order. The change results from the strong disposition or struggle of muriatic acid to undergo satu- ration, in consequence of which the hydro- cyanic acid and water suffer mutual decom- position. The nitrogen of the hydrocyanic acid and the hydrogen of the water unite together and form a base, ammonia, with which the acid unites; the chemical charac- ters of the acid being at the same time lost, because its desire for saturation is satisfied by its uniting with ammonia. Ammonia itself was not previously present, but only its elements, and the power to form it. The simultaneous decomposition of hydrocyanic acidf and water in this instance does not take place in consequence of the chemical affinity of muriatic acid for ammonia, since hydro- cyanic acid and water contain no ammonia. An affinity of one body for a second which is totally without the sphere of its attrac- tions, or which, so far as it is concerned, does not exist, is quite inconceivable. The ammonia in this case is formed only on ac- count of the existing attractive desire of the acid for saturation. Hence we may perceive how much these modes of decomposition, to which the name of transformations or meta- morphoses has been especially applied, differ from the ordinary chemical decompositions. 4 In consequence of the formation of am- monia, the other elements of hydrocyanic acid, namely, carbon and hydrogen, unite with the oxygen of the decomposed water, and form formic acid, the elements of this substance with the power of combination being present. Formic acid here represents the excrementitious matters ; ammonia, the new substance, assimilated by an organ of a plant or animal. Each organ extracts from the food pre- sented to it what it requires for its own sus- tenance; while the remaining elements, which are not assimilated, combine together and are separated as excrement. The ex- crementitious matters of one organ come in contact with another during their passage through the organism, and in consequence suffer new transformations ; the useless mat- ters rejected by one organ containing the elements for the nutrition of a second and a third organ : but at last, being capable of no farther transformations, they are separated from the system by the organs destined for that purpose. Each part of an organized being is fitted for its peculiar functions. A cubic inch of sulphuretted hydrogen intro- duced into the lungs would cause instant death, but it is formed, under a variety of circumstances, in the intestinal canal with- out any injurious effect. In consequence of such transformations as we have described, excrements are formed of various composition, some of these con- tain carbon in excess, others nitrogen, and others again hydrogen and oxygen. The kidneys, liver, and lungs, are organs of ex- cretion ; the first separate from the body all those substances in which a large propor- tion of nitrogen is contained ; the -second, those with an excess of carbon; and the third, such as are composed principally of oxygen and hydrogen. Alcohol, also, and the volatile oils which are incapable of be- ing assimilated, are exhaled through the lungs, and not through the skin. Respiration must be regarded as a slow process of combustion or constant decompo- sition. If it be subject to the laws which regulate the processes of decomposition gene- rally, the oxygen of the inspired air cannot combine directly with the carbon of com- pounds of that element contained in the blood ; the hydrogen only can combine with the oxygen of the air, or undergo a higher degree of oxidation. Oxygen is absorbed without uniting with carbon ; and carbonic acid is disengaged, the carbon and oxygen of which must be derived from matters pre- viously existing in the blood.* * The examination of the air expired by con- sumptive persons, as well as of their blood, would doubtless throw much light on the nature of phthisis pulmonaris. Considered in a chemical point of view, the decomposition of the blood, as it takes place in the lungs, is a true process of putrefac- tion. (See Part II.) The lungs are also the seat of the transformation of the various substances contained in the blood. It certainly well merits 26 AGRICULTURAL CHEMISTRY. All superabundant nitrogen is eliminated from the body,, as a liquid excrement, through the urinary passages ; all solid sub- stances, incapable of farther transformation, pass out by the intestinal canal, and all gaseous matter by the lungs. We should not permit ourselves to be withheld by the idea of a vital principle, from considering in a chemical point of view the process of the transformation of the food, and its assimilation by the various organs. This is the more necessary, as the views, hitherto held, have produced no results, and are quite incapable of useful application. Is it truly vitality, which generates sugar in the germ for the nutrition of young plants, or which gives to the stomach the power to dissolve, and to prepare for assimilation, all the matter introduced into if? A decoction of malt possesses as little power to repro- duce itself, as the stomach of a dead calf; both are, unquestionably, destitute of life. But when amylin or starch is introduced into a decoction of malt, it changes, first into a gummy-like matter, and lastly into sugar. Hard-boiled albumen and muscular fibre 1 can be dissolved in a decoction of a calf's stomach, to which a few drops of mu- riatic acid have been added, precisely as in the stomach itself.* (Schwann, Schulz.) The power, therefore, to effect transfor- mations, does not belong to the vital prin- ciple : each transformation is owing to a disturbance in the attraction of the elements of a compound, and is consequently a purely chemical process. There is no doubt that this process takes place in another form from, that of the ordinary decomposition of salts, oxides, or sulphurets. But is it the fault of chemistry that physiology has hith- erto taken no notice of this new form of chemical action ? j Physicians are accustomed to administer whole ounces of borax to patients suffering under urinary calculi, when it is known that the bases of all alkaline salts formed by organic acids are carried through the urinary passages in the form of alkaline carbonates, capable of dissolving calculi (Wohler.) Is this rational? The medical reports state, that upon the Rhine, where so much cream of tartar is consumed in wine, the only cases of calculous disorders are those which are imported from other districts. We know that the uric acid calculus is transformed consideration, that the most approved remedies for counteracting or stopping the progress of this frightful malady are precisely those which are found most efficacious in retarding putrefaction. Thus, it is well known that much relief is afforded by a residence in works in which ernpyreumatic oils are manufactured by dry distillation, such as manufactories lor the preparation of gas or sal-am- moniac. For the same reason, the respiration of wood vinegar (pyroligneous acid,) of chlorine, and certain 01 the acids, has been recognized as a means of alleviating the disease. * This remarkable action has been completely confirmed in this laboratory (Giessen,) by Dr. Vogel, a highly distinguished young physiologist. into the mulberry calculus (which contains oxalic acid,) when patients suffering undei the former exchange the town for the coun- try, where less animal and more vegetable food is used. Are all these circumstances incapable of explanation? The volatile oil of the roots of valerian may be obtained from the oil generated dur- ing the fermentation of potatoes (Dumas,) and the oil of the Spircea ulmaria from the crystalline matter of the bark of the willow (Piria.) We are able to form in our labor- atories formic acid, oxalic acid, urea, and the crystalline substances existing in the liquid of the allantois of the cow, all pro- ducts, it is said, of the vital principle. We see, therefore, that this mysterious principle has many relations in common with chemi- cal forces, and that the latter can indeed re- place it. What these relations are, it remains for physiologists to investigate. Truly it would be extraordinary if this vital principle, which uses every thing for its own purposes, had alloted no share to chemical forces, which stand so freely at its disposal. We shall obtain that which is obtainable in a rational inquiry into nature, if we se- parate the actions belonging to chemical powers from those which are subordinate to other influences. But the expression " vital principle" must in the mean time be consi- dered as of equal value with the terms spe- cific or dynamic in medicine : every thing is specific which we cannot explain, and dynamic is the explanation of all which we do not understand ; the terms having been invented merely for the purpose of conceal- ing ignorance by the application of learned epithets. Transformations of existing compounds are constantly taking place during the whole life of a plant, in consequence of which, and as the results of "these transformations, there are produced gaseous matters which are excreted by the leaves and blossoms, solid excrements deposited in the bark, and fluid soluble substances which are eliminated by the roots. Such secretions are most abun- dant immediately before the formation and during the continuance of the blossoms; they diminish after the development of the fruit. Substances containing a large propor- tion of carbon are excreted by the roots and absorbed by the soil. Through the expul- sion of these matters unfitted for nutrition, the soil receives again with usury, the car- bon which it had at first yielded to the young plants as food, in the form of car- bonic acid. The soluble matter thus acquired by the soil is still capable of decay and putrefaction, and by undergoing these processes furnishes renewed sources of nutrition to anothei gene- ration of plants; it becomes humus. The culti- vated soil is thus placed in a situation exactly analogous to that of forests and meadows, for the leaves of trees which fall in the forest in 'autumn, and the old roots of grass in the meadow, are likewise converted into humus ORGANIC CHEMICAL PROCESSES. 27 by the same influence : a soil receives more carbon in this form than its decaying humus had lost as carbonic acid. Plants do not exhaust the carbon of a soi in the normal condition of their growth j on the contrary, they add to its quantity. Bu if it is true that plants give back more car- bon to a soil than they take from it, it'is evi- dent that their growth "must depend upon the reception of nourishment from the atmo- sphere in the form of carbonic acid. The influence of humus upon vegetation is ex- plained by the foregoing facts in the most clear and satisfactory manner. Humus does not nourish plants by being taken up and assimilated in its unaltered state, but by presenting a slow and lasting source of carbonic acid, which is absorbed by the roots, and is the principal nutriment of young plants at a time when, being des- titute of leaves, they are unable to extract food from the atmosphere. In former periods of the earth's history,, its surface was covered with plants, the re- mains of which are still found in the coal formations. These plants the gigantic monocotyledons, ferns, palms, and reeds belong to a class to which nature has given the power, by means of an immense exten- sion of their leaves, to dispense with nour- ishment from the soil. They resemble in this respect the plants which we raise from bulbs and tubers, and which live while young upon the substances contained in their seed, and require no food from the soil when their exterior organs of nutrition are formed. This class of plants is even at present ranked amongst those which do not exhaust the soil. The necessity of the existence of plants such as these at the commencement of ve- getation, must now be apparent. Humus is a product of the decay of vegetable mat- ter, and therefore could not have existed to supply the first plants with the food neces- sary for the development of the more deli- cate kinds. Hence the plants capable of flourishing under such circumstances could only be those which receive their nourish- ment from the air alone. By their decay, however, the soil in which they grew be- came supplied with vegetable matter, and the progress of vegetation must have fur- nished to the earth materials adapted for the development of those plants, which depend upon the nutriment contained in the soil, until those organs are formed which are des- tined for the assumption of nourishment from the atmosphere. The plants of every former period are dis- tinguished from those of the present by the inconsiderable development of their roots. Fruit, leaves, seeds, nearly every part of the plants of a' former world, except the roots, are found in the brown coal formation. The vascular bundles, and the perishable cellular tissue, of which their roots consisted, have been the first to suffer decomposition. But when we examine oaks and other trees, which in consequence of revolutions of the same kind occurring in later ages have un- dergone the same changes, we never find their roots absent. The verdant plants of warm climates are very often such as obtain from the soil only a point of attachment, and are not dependent on it for their growth. How extremely small are the roots' of the Cactus, Sedum, and Sempemimtm, in proportion to their mass, and to the surface of their leaves! Large forests are often found growing in soils absolutely destitute of carbonaceous matter; and the extensive prairies of t'io western continent show that the carbon necessary for the sustenance of a plant may be entirely extracted from the atmosphere. Again, in the most dry and barren sand, where it is impossible for nourishment to be obtained through the roots, we see the milky- juiced plants attain complete perfection. The moisture necessary for the nutrition of these plants is derived from the atmosphere, and when assimilated is secured from eva- poration by the nature of the juice itself.. Caoutchouc and wax, which are formed in these plants, surround the water, as in oily emulsions, with an impenetrable envelope by*which the fluid is retained, in the same manner as milk is prevented from evaporat- ing by the skin which forms upon it. These plants, therefore, become turgid with their juices. Particular examples might be cited of plants, which have been brought to maturity, upon a small scale, without the assistance of mould ; but fresh proofs of the accuracy of our theory respecting the origin of carbon would be superfluous and useless, and could not render more striking, or more con- vincing, the arguments already adduced. It must not, however, be left unmentioned, that common wood charcoal, by virtue merely of its ordinary well-known proper- ties, can completely replace vegetable mould or humus. The experiments of Lukas, which are appended to this work, spare 'me all further remarks upon its efficacy. Plants thrive in powdered charcoal, and may be brought to blossom and bear fruit if exposed to the influence of the rain and the atmosphere; the charcoal may be previously iieated to redness. Charcoal is the most indifferent" and most unchangeable sub- stance known ; it may be kept for centuries without change, and is, therefore, not sub- ject to decomposition. The only substances which it can yield to plants are some salts, which it contains, amongst which is silicate of potash. It is known, however, to pos- sess the power of condensing gases within its pores, and particularly carbonic acid. And it is by virtue of this power that the roots of plants are supplied in charcoal, ex- actly as in humus, with an atmosphere of carbonic acid and air, which is renewed as quickly as it is abstracted. In charcoal powder, which had been used br this purpose by Lukas for several AGRICULTURAL CHEMISTRY. Buehner found a brown substance soluble in alkalies. This substance was evidently due to the secretions from the roots of the plants which grew in it. A plant placed in a closed vessel in which the air., and therefore the carbonic acid, can- not be renewed, dies exactly as it would do in the vacuum of an air-pump, or in an at- mosphere of nitrogen or carbonic acid, even though its roots be fixed in the richest mould. Plants do not, however, attain maturity, under ordinary circumstances, in charcoal powder, when they are moistened with pure distilled water instead of rain or river water. Rain water must, therefore, contain within it one of the essentials of vegetable life ; and it will be shown, that this is the presence of a compound containing nitrogen, the exclu- sion of which entirely deprives humus and charcoal of their influence upon vegetation. CHAPTER IV. ON THE ASSIMILATION OF HYDROGEN. THE atmosphere contains the principal food of plants in the form of carbonic acid, in the state, therefore, of an oxide. The solid part of plants (woody fibre) contains carbon and the constituents of water, or the elements of carbonic acid, together with a .certain quantity of hydrogen. It has for- merly been mentioned that water consists of the two gases, oxygen and hydrogen. The range of affinity possessed by both these elements is so extensive that numerous causes occur which effect the decomposition of water. Indeed, there is no compound which plays a more general or more im- portant part in the phenomena of combina- tion and decomposition. We can conceive the wood to arise from a combination of the carbon of the carbonic acid with the elements of water, under the influence of solar light. In this case, 72.35 parts of oxygen, by weight, must be separated as a gas for every 27.65 parts of carbon, which are assimilated by a plant; for this is the composition of carbonic acid in 100 parts. Or, what is much more probable, plants, under the same circum- stances, may decompose water, the hydro- gen of which is assimilated along with car- bonic acid, whilst its oxygen is separated. If the latter change takes place, 8.04 parts of hydrogen must unite with 100 parts of carbonic acid, in order to form woody fibre, and the 72.35 parts by weight of oxygen, which was in combination with the hydro- gen of the water, and which exactly corre- sponds in quantity with the oxygen contained in the carbonic acid, must be separated in a gaseous form. Each acre of land, which produces 10 cwts. of carbon, gives annually to the at- mosphere 865 Ibs. of free oxygen gas. The specific weight of oxygen is expressed by the number 1.1026; hence 1 cubic metre of oxygen weighs 3.157 Ibs., and 2865 Ibs. of oxygen correspond to 908 cubic metres, or 32,007 cubic feet. An acre of meadow, wood, or cultivated land in general replaces, therefore, in the atmosphere as much oxygen as is exhausted by 10 cwts. of carbon, either in its ordinary combustion in the air or in the respiratory- process of animals. It has been mentioned at a former page that pure woody fibre contains carbon and the component parts of water, but that ordi- nary wood contains more hydrogen than corresponds to this proportion. This excess is owing to the presence of the green princi- ple of the leaf, wax, resin, and other bodies rich in hydrogen. Water must be decom- posed, in order to furnish the excess of this element, and consequently one equivalent of oxygen must be given back to the atmosphere for every equivalent of hydrogen appropri- ated by a plant to the production of those sub- stances. The quantity of oxygen thus set at liberty cannot be insignificant, for the at- mosphere must receive 989 cubic feet of oxygen for every pound of hydrogen assi- milated. It has already been stated, that a plant, in the formation of woody fibre, must always yield to the atmosphere the same propor- tional quantity of oxygen; that the volume of this gas set free would be the same whether it were due to the decomposition of carbonic acid or of water. A little consi- deration will show that this must be the case. It has repeatedly been stated, that woody fibre contains carbon in combination with oxygen and hydrogen in the same propor- tion in which they exist in water. Water contains 1 equivalent of each element, whilst carbonic acid consists of 1 equivalent of carbon, united to 2 equivalents of oxygen. In the formation of woody fibre, 2 equiva- lents of oxygen must therefore be libe- rated. The woody fibre can only be formed in one of two ways : either the car- bon of carbonic acid unites directly with water, or the hydrogen of water combines with the oxygen of the carbonic acid. In the former of these cases, the two equiva- lents of oxygen in the carbonic acid must be liberated ; in the latter, two atoms of water must be decomposed, the hydrogen of which unites with the oxygen of the carbonic acid, whilst the oxygen of the water, thus set free, is disengaged in the state of a gas. It was considered most probable that the latter was the case. From their generating caoutchouc, wax, fats, and volatile oils containing hydrogen in large quantity, and no oxygen, we may be certain that plants possess the property of decomposing water, because from no other body could they obtain the hydrogen of those matters. It has also been proved by the observations of Humboldt on the fungi, that water may be decomposed with- out the assimilation of hydrogen. Water is a remarkable combination of two elements, ASSIMILATION OF HYDROGEN. which have the power to separate them- selves from one another, in innumerable processes, in a manner imperceptible to our ( senses j while carbonic acid, on the contrary, is only decomposable by violent chemical action. Most vegetable structures contain hydro- gen in the form of water, which can be sepa- rated as such, and replaced by other bodies ; but the hydrogen which is essential to their constitution cannot possibly exist in the state of water. All the hydrogen necessary for the forma- tion of an organic compound is supplied to a plant by the decomposition of water. The process of assimilation, in its most simple form, consists in the extraction of hydrogen from water, and carbon from carbonic acid, in consequence of which, either all the oxy- gen of the water and carbonic acid is sepa- rated, as in the formation of caoutchouc, the volatile oils which contain no oxygen, and other similar substances, or only a part of it is exhaled. The known composition of the organic compounds most generally present in vege- tables, enables us to state in definite propor- tions the quantity of oxygen separated during their formation. 36 eq. carbonic acid and^ 22 eq. hydrogen derived >= Woody Fibre, from 22 eq. water. 3 with the separation of 72 eq. oxygen. 36 eq. carbonic acid and^ 36 eq. hydrogen derived >= Sugar, from 36 eq. water with the separation of 72 eq. oxygen. 36 eq. carbonic acid and^ 30 eq. hydrogen derived >= Starch, from 30 eq. water 3 with the separation of 72 eq. oxygen. 36 eq. carbonic acid and^ 16 eq. hydrogen derived >= Tannic Acid, from 16 eq. water 3 with the separation of 64 eq. oxygen. 36 eq. carbonic acid and^ 18 eq. hydrogen derived {= Tartaric Acid, from 18 eq. water 3 with the separation of 45 eq. oxygen. 36 eq. carbonic acid and^ 18 eq. hydrogen derived >= Malic Acid, from 18 eq. water 3 with the separation of 54 eq. oxygen. 36 eq. carbonic acid and) 24 eq. hydrogen derived > = Ot'Z of Turpentine. from 24 eq. water ) with the separation of 84 eq. oxygen. It will readily be perceived that the for- mation of the acids is accompanied with the smallest separation of oxygen; that the amount of oxygen set free increases with the production of the so-named neutral sub- stances, and reaches its maximum in the formation of the oils. Fruits remain acid in cold summers ; while the most numerous trees under the tropics are those which pro- duce oils, caoutchouc, and other substances containing very little oxygen. The action of sunshine and influence of heat upon the ripening of fruit is thus, in a certain mea- sure, represented by the numbers above uted. The green resinous principle of the leaf diminishes in quantity, while oxygen is ab- sorbed, when fruits are ripened in the dark j red and yellow colouring matters are formed ; tartaric, citric, and tannic acids disappear, and are replaced by sugar, amylin, or gum. 6 eq. Tartaric Acid, by absorbing 6 eq. oxygen from the air, form Grope Sugar, with the separation of 12 eq. carbonic acid. 1 eq. Tannic Jlcid, by absorbing 8 eq. oxy- gen from the air, and 4 eq. water, form 1 eq. of Jlmylin, or starch, with separation of 6 eq. carbonic acid. We can explain, in a similar manner, the formation of all the component substances of plants which contain no nitrogen, whether they are produced from carbonic acid and water, with separation of oxygen, or by the conversion of one substance into the other, by the assimilation of oxygen and separation of carbonic acid. We do not know in what form the production of these constituents takes place ; in this respect, the representa- tion of their formation which we have given must not be received in an absolute sense, it being intended only to render the nature of the process more capable of apprehension ; but it must not be forgotten, that if the con- version of tartaric acid into sugar, in grapes, be considered as a fact, it must take place under all circumstances in the same propor- tions. The vital process in plants is, with refer- ence to the point we have been considering, the very reverse of the chemical processes engaged in the formation of salts. Carbonic acid, zinc, and water, when brought into contact, act upon one another, and hydrogen is separated, while a white pulverulent compound is formed, which contains car- bonic acid, zinc, and the oxygen of the water. A living plant represents the zinc in this process : but the process of assimila- tion gives rise to compounds, which contain the elements of carbonic acid and the hydro- gen of water, whilst oxygen is separated. Decay has been described above as the great operation of nature, by which that oxygen, which was assimilated by plants during life, is again returned to the atmo- sphere. During the progress of growth, plants appropriate carbon in the form of car- bonic acid, and hydrogen from the decom- position of water, the oxygen of which is set free, together with a part of all that con- tained in the carbonic acid. In the process of putrefaction, a quantity of water, exactly corresponding to that ot tfc.e hydrogen, is again formed by extraction of oxygen from the air; while all the oxygen of the organic matter is returned to the atmosphere in the form of carbonic acid. Vegetable matters can emit carbonic acid, during their decay, only in proportion to the quantity of oxygen which they contain; acids, therefore, yield more carbonic acid than neutral compounds ; while fatty acids, resin, and wax, do not putrefy ; they remain in the soil without any apparent change. so AGRICULTURAL CHEMISTRY. The numerous springs which emit car- bonic acid in the neighbourhood of extinct volcanoes, must he regarded as another means of compensating for the carbonic acid absorbed and retained by plants during life, and consequently as a source by which oxy- gen is supplied to the atmosphere. Bischof calculated that the springs of carbonic acid in the Eifel (a volcanic district near Cob- lenz) send into the air every day more than 110,000 Ibs. of carbonic acid, corresponding to 79,000 Ibs. of pure oxygen. CHAPTER V. ON THE ORIGIN AND ASSIMILATION OP NITROGEN. WE cannot suppose that a plant could attain maturity, even in the richest vege- table mould, without the presence of matter containing nitrogen; since we know that nitrogen exists in every part of the vegetable structure. The first and most important question to be solved, therefore, is: How and in what form does nature furnish nitro- gen to vegetable albumen, and gluten, to fruits and seeds? This question is susceptible of a very simple solution. Plants, as we know, grow perfectly well in pure charcoal, if supplied at the same time with rain water. Rain water can con- tain nitrogen only in two forms, either as dissolved atmospheric air, or as ammonia, which consists of this element and hydro- gen. Now, the nitrogen of the air cannot be made to enter into combination with any element except oxygen, even by the employ- ment of the most powerful chemical means. We have not the slightest reason for believ- ing that the nitrogen of the atmosphere takes part in the processes of assimilation of plants and animals ; on the contrary, we know that many plants emit the nitrogen which is absorbed by their roots, either in the gaseous form, or in solution in water. But there are on the other hand numerous facts, showing, that the formation in plants of substances containing nitrogen, such as gluten, takes place in proportion to the quantity of this element which is conveyed to their roots in the state of ammonia, de- rived from the putrefaction of animal matter. Ammonia, toq, is capable of undergoing such a multitude of transformations, when in contact with other bodies, that in this respect it is not inferior to water, which pos- sesses the same property in an eminent de- gree. It possesses properties which we do not find in any other compound of nitrogen : when pure, it is extremely soluble in water; it forms soluble compounds with all the acids; and when in contact with certain other substances, it completely resigns its character as an akali, and is capable of as- suming the most various and opposite forms Formate of ammonia changes, under the influence of a high temperature, into hy- drocyanic acid and water, without the sepa- ration of any of its elements. Ammonia forms urea, with cyanic acid, and a series of crys- talline compounds, with the \olatile oils of mustard and bitter almonds. It changes into splendid blue or red colouring matters, when in contact with the bitter constituent of the bark of the apple-tree (phloridzin,) with the sweet principle of the Variolaria dealba,ta(orcin,} or with the tasteless matter of the Rocella tinctoria (erythrin.) All blue colouring matters which are reddened by acids, and all red colouring substances which are rendered blue by alkalies, contain nitrogen, but not in the form of a base. These facts are not sufficient to establish the opinion that it is ammonia which affords all vegetables, without exception, the nitro- gen which enters into the composition of their constituent substances. Considerations of another kind, howeves, give to this opi- nion a degree of certainty which completely excludes all other views of the matter. Let us picture to ourselves the condition of a well-cultured farm, so large as to be in- dependent of assistance from other quarters. On this extent of land there is a certain quantity of nitrogen contained both in the corn and fruit which it produces, and in the men and animals which feed upon them, and also in their excrements. We shall suppose this quantity to be known. The land is cultivated without the importation of any foreign substance containing nitro- gen. Now, the products of this farm must be exchanged every year for money, and other necessaries of life for bodies, there- fore, which contain no nitrogen. A certain proportion of nitrogen is exported with corn and cattle ; and this exportation takes place every year, without the smallest compensa- tion ; yet after a given number of years, the quantity of nitrogen will be found to have increased. Whence, we may ask, comes this increase of nitrogen? The nitrogen in the excrements cannot reproduce itself, and the earth cannot yield it. Plants, and con- sequently animals, must, therefore, derive their nitrogen from the atmosphere. It will in a subsequent part of this work be shown that the last products of the decay and putrefaction of animal bodies present themselves in two different forms. They are in the form of a combination of hydro- gen and nitrogen ammonia in the temper- ate and cold climates, and in that of a com- pound containing oxygen nitric acid in the tropics and hot climates. The forma- tion of the latter is preceded by the produc- tion of the first. Ammonia is the last pro- duct of the putrefaction of animal bodies; nitric acid is the product of the transforma- tion of ammonia. A generation of a thou- sand million men is renewed every thirty years : thousands of millions of animals cease to live and are reproduced, in a much ASSIMILATION OF NITROGEN. shorter period. Where is the nitrogen which they contained during life? There is no question which can be answered with more positive certainty. All animal bodies during their decay yield the nitrogen which they contain to the atmosphere, in the form of ammonia. Even in the bodies buried sixty feet under ground in the churchyard of the Eglise des Innocens, at Paris, all the nitro- gen contained in the adipocire was in the slate of ammonia. Ammonia is the simplest of all the compounds of nitrogen ; and hy- drogen is the element for which nitrogen possesses the most powerful affinity. The nitrogen of putrified animals is con- tained in the atmosphere as ammonia, in the form of a gas which is capable of entering into combination with carbonic acid and of forming a volatile salt. Ammonia in its gaseous form, as well as all its volatile com- pounds, is of extreme solubility in water. Ammonia, therefore, cannot remain long in the atmosphere, as every shower of rain must condense it, and convey it to the sur- face of the earth. Hence, also, rain-water must at all times contain ammonia, though not always in equal quantity. It must be greater in summer than in spring or in win- ter, because the intervals of time between the showers are in summer greater; and when several wet days occur, the rain of the first must contain more of it than that of the second. The rain of a thunder storm, after a long-protracted drought, ought for this reason to contain the greatest quantity which is conveyed to the earth at one time. But we have formerly stated, that all the analyses of atmospheric air hitherto made have failed to demonstrate the presence of ammonia, although, according to our view, it can never be absent. Is it possible that it could have escaped our most delicate and most exact apparatus ? The quantity of ni- trogen contained in a cubic foot of air is certainly extremely small, but, notwithstand- ing this, the sum of the quantities of nitro- gen from thousands and millions of dead animals is more than sufficient to supply all those living at one time with this element. From the tension of aqueous vapour at 150 C . (590 F.)=6,98 lines (Paris mea- sure,) and from its known specific gravity at C. (32 F.,) it follows that when the temperature of the air is 59 F. and the height of the barometer 28", 1 cubic metre or 35.3 cubic feet of aqueous vapour are contained in 487 cubic metres, or 17,191 cubic feet of air: 35.3 cubic feet of aqueous vapour weigh about 1 $ Ib. Consequently, if we suppose that the air saturated with moisture at 59 F. allows all the water which it contains in the gaseous form to fall as rain, then 1.1 pound of rain-water must be obtained from every 11,471 cubic feet of air. The whole quantity of ammonia con- tained in the same number of cubic feet will also be returned to the earth in this one pound of rain-water. But if the 11,471 cubic feet of air contain a single grain of ammonia, then ten cubic inches the quan- tity usually employed in an analysis must contain only O.OOOQOQ048 of a grain. This extremely small proportion is absolutely in- appreciable by the most delicate and best eudiometer ; it might be classed among the errors of observation, even were its quan- ity ten thousand times greater. But the detection of ammonia must be much more easy when a pound of rain-water is ex- amined, for this contains all the gas that was diffused through 11,471 cubic feet of air. If a pound of rain-water contain only th of a grain of ammonia, then a field of 26,910 ?uare feet must receive annually upwards 88 Ibs. of ammonia, or 71 Ibs. of nitro- gen; for by the observations of Schubler, which were formerly alluded to, about 770,000 Ibs. of rain fall over this surface in four months, and consequently the annual fall must be 2,310,000 Ibs. This is much more nitrogen than is contained in the form of vegetable albumen and gluten, in 2920 Ibs. of wood, 3085 Ibs. of hay, or 200 cwL of beet-root, which are the yearly produce of such a field ; but it is less than the straw, roots, and grain of corn, which might grow on the same surface, would contain.* Experiments made in this laboratory (Giessen) with the greatest care and exact- ness have placed the presence of ammonia in rain-water beyond all doubt. It has hi- therto escaped observation, because no per- son thought of searching for it. All the rain-water employed in this inquiry was col- lected 600 paces south-west of Giessen, whilst the wind was blowing in the direc- tion of the town. When several hundred pounds of it were distilled in a copper still, and the first two or three pounds evaporated with the addition of a little muriatic acid, a very distinct crystallisation of sal-ammoniac was obtained: the crystals had always a brown or yellow colour. Ammonia may likewise be always detected in snow-water. Crystals of sal-ammoniac * The advocates of the importance of humus as a nourishment for plants, being driven from their position by the facts brought forward in the pre- ceding chapters, have found in the ammonia of the atmosphere an explanation of the manner in which humus acquires its solubility, and therefore its ca- pability of being assimilated by plants. Now, it is very true that humic acid is soluble in ammo- nia ; but the humic acid of chemists is not con- tained in soils. Were it so, on treating mould with water we should obtain a dark-coloured so- lution of humate of ammonia. But we obtain a solution which is entirely devoid of this acid. It cannot be too distinctly kept in mind that humic acid is the product of the decomposition of humus, by means of caustic alkalies. Again, if the coloured solutions of humates of ammonia, lime', or magnesia, be poured upon good mould or de- cayed oak-wood (which is nearly pure humus,} and allowed to filter, the solutions are observed to pas through quite colourless ; they are decolourised just as if they had been filtered through charcoal. Here, then, humus possesses the property of ex- tracting humic acid from water ; or, in other words, soils have the power of rendering humic acid in- soluble, or unfit for assimilation. ED. 32 AGRICULTURAL CHEMISTRY. were obtained by evaporating in a vessel with muriatic acid several pounds of snow, which were gathered from the surface of the ground in March, when the snow had a depth of 10 inches. Ammonia was set free from these crystals by the addition of hydrate of lime. The inferior layers of snow which rested upon the ground contained a quantity decidedly greater than those which formed the surface. It is worthy of observation that the am- monia contained in rain and snow water possesses an offensive smell of perspiration and animal excrements., a fact which leaves no doubt respecting its origin. Hunefield has proved that all the springs in Greifswalde, Wick, Eldena, and Kosten- hagen, contain carbonate and nitrate of am- monia. Ammoniacal salts have been disco- vered in many mineral springs in Kissingen and other places. The ammonia of these salts can only arise from the atmosphere. Any one may satisfy himself of the pre- sence of ammonia in rain by simply adding a little sulphuric or muriatic acid to a quan- tity of rain-water, and evaporating this nearly to dryness in a clean porcelain basin. The ammonia remains in the residue, in combination with the acid employed; and may be detected either by the addition of a little chloride of platinum, or more simply by a little powdered lime, which separates the ammonia, and thus renders its peculiar pungent smell sensible.* The sensation which is perceived upon moistening the hand with rain-water, so different from that produced by pure distilled water, and to which the term softness is vulgarly applied, is also due to the carbonate of ammonia contained in the former. The ammonia which is removed from the atmosphere by rain and other causes, is as constantly replaced by the putrefaction of animal and vegetable matters. A certain portion of that which falls with the rain evaporates again with the water, but another portion is, we suppose, taken up by the roots of plants, and entering into new com- binations in the different organs of assimila- tion, produces albumen, gluten, quinine, morphia, cyanogen, and a number of other compounds containing nitrogen. The chemi- cal characters of ammonia render it capable of entering into such combinations, and of undergoing numerous transformations. We have rfow only to consider whether it really * Since the appearance of the last edition, this experiment has been repeated by many in France, Germany, America, and England, and the exist- ence of ammonia in the atmosphere has been completely confirmed. The assertion that this ammonia possesses the "offensive smell of per- spiration and animal excrements," has been ridi- culed by many as fanciful by none, however, who have made the experiment. The experiment is so exceedingly easy to perform, that any one may convince himself of the accuracy of the state- ment. ED; is taken up in the form of ammonia by the roots of plants, and in that form applied by their organs to the production of the azotised matters contained in them. This question is susceptible of easy solution by well-known facts. In the year 1834, 1 was engaged with Dr. Wilbrand, professor of botany in the uni- versity of Giessen, in an investigation re- specting the quantity of sugar contained in different varieties of maple-trees, which grew upon soils which were not manured. We obtained crystallised sugars from all, by simply evaporating their juices, without the addition of any foreign substance ; and we unexpectedly made the observation, that a great quantity of ammonia was emitted from this juice when mixed with lime, and also from the sugar itself during its refinement. The vessels which hung upon the trees in order to collect the juice were watched with greater attention, on account of the sus- picion that some evil-disposed persons had introduced urine into them, but still a large quantity of ammonia was again found in the form of neutral salts. The juice had no colour, and had no reaction on that of vege- tables. Similar observations were made upon the Juice of the birch tree; the specimens subjected to experiment were taken from a wood several miles distant from any house, and yet the clarified juice, evaporated witl/. lime, emitted a strong odour of ammonia. In the manufactories of beet-root sugar, many thousand cubic feet of juice are daily purified with lime, in order to free it from vegetable albumen and gluten, and it is afterwards evaporated for crystallisation. Every person who has entered such a manufactory must have been astonished at the great quantity of ammonia which is volatilised along with the steam. This am- monia must be contained in the form of an ammoniacal salt, because the neutral juice possesses the same characters as the solu- tion of such a salt in water ; it acquires, namely, an acid reaction during evaporation, in consequence of the neutral salt being con- verted by loss of ammonia into an acid salt The free acid which is thus formed is a source of loss to the manufacturers of sugar from beet-root, by changing a part of the sugar into uncrystallisable grape sugar and syrup. The products of the distillation of flowers, herbs, and roots, with water, and all ex- tracts of plants made for medicinal purposes, contain ammonia. The unripe, the trans- parent, and gelatinous pulp of the almond and peach emit much ammonia when treated with alkalies. (Robiquet.) The juice of the fresh tobacco leaf contains ammoniaca. salts. The water which exudes from a cu vine, when evaporated with a few drops of muriatic acid, also yields a gummy deli- quescent mass, which evolves much ammo- nia on the addition of lime. Ammonia exisst in every part of plants, in the roots (as in ASSIMILATION OF NITROGEN. S3 beet-root,) in the stem (of the maple-tree,) and in all blossoms and fruit in an unripe condition. The juices of the maple and birch contain both sugar and ammonia, and therefore afford all the conditions necessary for the formation of the azotised components of the branches, blossoms, and leaves, as well as of those which contain no azote or nitrogen. In proportion as the developement of those parts advances, the ammonia diminishes in quantity, and when they are fully formed,, the tree yields no more juice. The employment of animal manure in the cultivation of grain, and the vegetables which serve for fodder to cattle, is the most convincing proof that the nitrogen of vege- tables is derived from ammonia. The quantity of gluten in wheat, rye, and bar- ley, is very different ; these kinds of grain also, even when ripe, contain this compound of nitrogen in very different proportions. Proust found French wheat to contain 12.5 per cent, of gluten; Vogel found that the Bavarian contained 24 per cent.; Davy ob- tained 19 per cent, from winter, and 24 from summer wheat; from Sicilian 21, and from Barbary wheat 19 per cent. The meal of Alsace wheat contains, according to Bous- singault, 17.3 per cent, of gluten; that of wheat grown in the " Jardin des Plantes" 26.7, and that of winter wheat 3.33 per cent. Such great differences must be owing to some cause, and this we find in the diffe- rent methods of cultivation. An increase of animal manure gives rise not only to an in- crease in the number of seeds, but also to a most remarkable difference in the proportion of the substances containing nitrogen, such as the gluten which they contain. Animal manure, in as far as regards the assimilation of nitrogen, acts only by the formation of ammonia. One hundred parts of wheat grown on a soil manured with cow-dung fa manure containing the smallest quantity of nitrogen,) afforded only 11.95 parts of gluten, and b4.34 parts of amylhi, or starch ; whilst the same quantity, grown on a soil manured with human urine, yielded the maximum of gluten, namely 35.1 per cent. Putrefied urine contains nitrogen in the forms of carbonate, phosphate, and lac- tate of ammonia, and in no other form than that of ammoniacal salts. " Putrid urine is employed in Flanders as a manure with the best results. During the putrefaction of urine, ammoniacal salts are formed in large quantity, it may be said ex- clusively; for under the influence of heat and moisture, urea, the most prominent in- gredient of the urine, is converted into car- bonate of ammonia. The barren soil on the coast of Peru is rendered fertile by means of a manure called Guano, which is collected from several islands in the South Sea.* It is sufficient to add a small quantity of guano * The guano, which forms a stratum several feet in thickness upon the surface of these islands, consists of the putrid excrements of innumerable 5 to a soil, which consists only of sand and clay, in order to procure the richest crop of maize. The soil itself does not contain the smallest particle of organic matter, and the manure employed is formed only of urate, phosphate, oxalate, and carbonate of ammonia, together with a few earthy salts."* Ammonia, therefore, must have yielded the nitrogen to these plants. Gluten is ob- tained not only from corn, but also from grapes and other plants ; but that extracted from the grapes is called vegetable albumen, although it is identical in composition and properties with ^he ordinary gluten. It is ammonia which yields nitrogen to the vegetable albumen, the principal con- stituent of plants ; and it must be ammonia which forms the red and blue colouring matters of flowers. Nitrogen is not pre- sented to wild plants in any other form ca- pable of assimilation. Ammonia, by its transformation, furnishes nitric acid to the tobacco plant, sun-flower, Chenopodium, and Borago ojficinalis, when they grow in a soil completely free from nitre. Nitrates are necessary constituents of these plants, which thrive only when ammonia is present in large quantity, and when they are also subject to the influence of the direct rays of the sun, an influence necessary to effect the disengagement within their stem and leaves of the oxygen, which shall unite with the ammonia to form nitric acid. The urine of men and of carnivorous animals contains a large quantity of nitrogen, partly in the form of phosphates, partly as urea. Urea is converted during putrefac- tion into carbonate of ammonia, that is ttf say, it takes the form of the very salt which occurs in rain-water. Human urine is the most powerful manure for all vegetables containing nitrogen ; that of horses and tiorned cattle contains less of this element, but infinitely more than the solid excrements of these animals. In addition to urea, the urine of herbivorous animals contains hip- puric acid which is decomposed during pu refaction into benzoic acid and ammonia. The latter enters into the composition of the gluten, but the benzoic acid often remains unchanged : for example, in the Jlnthoxan,- l hum odoratum. The solid excrements of animals contain comparatively very little nitrogen, but this could not be otherwise. The food taken by animals supports them only in so far as it offers elements for assimilation to the various organs which they may require fcir their ncrease or renewal. Corn, grass, and all plants, without exception, contain azotised substances. The quantity of food which animals take for their nourishment, dimi- nishes or increases in the same proportion as it contains more or less of the substances containing nitrogen. A horse may be kept sea fowl that remain on them during the breeding season. See the Chapter on Manures.) * Boussingault, Ann. de Ch. et de Phys. Lev. p. 34 AGR1CJLTURAL CHEMISTRY. alive by feeding it with potatoes, which con- tain a very small quantity of nitrogen ; but life thus supported is a gradual starvation; the animal increases neither in size nor strength, and sinks under every exertion. The quantity of rice which an Indian eats astonishes the European ; but the fact that rice contains less nitrogen than any other 1 kind of grain at once explains the circum- stance. - Now, as it is evident that the nitrogen of the plants and seeds used by animals as food must be employed in the process of assimila- tion, it is natural to expect that the excre- ments of these animals will be deprived of it in proportion to the perfect digestion of the food, and can only contain it when mixed with secretions from the liver and intestines. Under all circumstances, they must contain less nitrogen than the food. When, there- fore, a field is manured with animal excre- ments, a smaller quantity of matter contain- ing nitrogen is added to it than has been taken from it in the form of grass, herbs, or seeds. By means of manure, an addition only is made to the nourishment which the air supplies. In a scientific point of view, it should be the care of the agriculturist so to employ all the substances containing a large proportion of nitrogen which his farm affords in the form of animal excrements, that they shall serve as nutriment to his own plants. This will not be the case unless those substances are properly distributed upon his land. A heap of manure lying unemployed upon his land would serve him no more than his neighbours. The nitrogen in it would es- cape as carbonate of ammonia into the at- mosphere, and a mere carbonaceous residue of decayed plants would, after some years, be found in its place. All animal excrements emit carbonic acid and ammonia, as long as nitrogen exists in them. In every stage of their putrefaction an escape of ammonia from them may be induced by moistening them with a potash ley; the ammonia being apparent to the senses by a peculiar smell, and by the dense white vapour which arises when a solid body moistened with an acid is brought near it. This ammonia evolved from manure is imbibed by the soil either in solution in water, or in the gaseous form, and plants thus receive a larger supply of nitrogen than is afforded to them by the atmosphere. But it is much less the quantity of am- monia, yielded to a soil by animal excre- ments, than the form in which it is presented by them, that causes their great influence on its fertility. Wild plants obtain more nitrogen from the atmosphere in the form of ammonia than they require for their growth, for the water which evaporates through their leaves and blossoms, emits, after some time, a putrid smell, a peculiarity possessed only by such bodies as contain nitrogen. Culti- vated plants receive the same quantity of nitrogen from the atmosphere as trees, shrubs, and other wild plants; but this is not sufficient for the purposes of agricul- ture. Agriculture differs essentially from the cultivation of forests, inasmuch as its principal object consists in the production of nitrogen under any form capable of as- similation ; whilst the object of forest culture is confined principally to the production of carbon. All the various means of culture are subservient to these two main purposes. A part only of the carbonate of ammonia which is conveyed by rain to the soil is re- ceived by plants, because a certain quantity of it is volatilised with the vapour of water ; only that portion of it can be assimilated which sinks deeply into the soil, or which is conveyed directly to the leaves by dew, or is absorbed from the air along with the car- bonic acid. Liquid animal excrements, such as the urine with which the solid excrements are impregnated, contain the greatest part of their ammonia in the state of salts, in a form, therefore, in which it has completely lost its volatility; when presented in this condition, not the smallest portion of the ammonia is lost to the plants; it is all dissolved by water, and imbibed by their roots. The evident influence of gypsum upon the growth of grasses the striking fertility and luxuriance of a meadow upon which it is strewed depends only upon its fixing in the soil the ammonia of the atmosphere, which would otherwise be volatilized, with the water which evaporates.* The carbonate of am- monia contained in rain-water is decom- posed by gypsum, in precisely the same manner as in the manufacture of sal-am- moniac. Soluble sulphate of ammonia and carbonate of lime are formed ; and this salt of ammonia possessing no volatility is con- sequently retained in the soil. All the gyp- sum gradually disappears, but its action upon the carbonate of ammonia continues as long as a trace of it exists. The beneficial influence of gypsum and of many other salts has been compared to that of aromatics, which increase the activity of the human stomach and intestines, and give a tone to the whole system. But plants con- tain no nerves ; we know of no substance capable of exciting them to intoxication and madness, or of lulling them to sleep and re- pose. No substance can possibly cause their leaves to appropriate a greater quantity of carbon from the atmosphere, when the other constituents which the seeds, roots, and leaves require for their growth are wanting. The favourable action of small quantities of aromatics upon man, when mixed with his food, is undeniable ; but aromatics are given to plants without food to be digested, and still they flourish with greater luxuriance. * It has long been the practice in some parts of the country to strew the floors of stables with gypsum. This prevents the disagreeable odour arising from the putrefaction of stable manure, by decomposing the ammoniacal salts which are formed. -ED. ASSIMILATION OP NITROGEN". 35 It is quite evident, therefore, that the common view concerning- the influence of certain salts upon the growth of plants evinces only ignorance of its cause. The action of gypsum or chloride of cal- ciuin really consists in their giving a fixed condition to the nitrogen or ammonia which is brought into the soil, and which is indispensable for the nutrition of plants. In order to form a conception of the effect of gypsum, it may be sufficient to remark that 110 Ibs. of burned gypsum fixes as much ammonia in the soil as 6880 Ibs. of horse's urine* would yield to it, even on the supposition that all the nitrogen of the urea and hippuric acid were absorbed by the plants without the smallest loss, in the form of carbonate of ammonia. If we admit with Boussingaultf that the nitrogen in grass amounts to y-J-j- of its weight, then every pound of nitrogen which we add increases the produce of the meadow 100 Ibs., and this increased produce of 100 Ibs. is effected by the aid of a little more than 4 Ibs. of gypsum. Water is absolutely necessary to effect the decomposition of the gypsum, on account of its difficult solubility, (1 part of gypsum requires 400 parts of water for solution) and also to assist in the absorption of the sul- phate of ammonia by the plants : hence it happens, that the influence of gypsum is not observable on dry fields and meadows. In such it would be advisable to employ a salt of more easy solubility, such as chloride of calcium. The decomposition of gypsum by carbo- nate of ammonia does not take place instan- taneously ; on the contrary, it proceeds very gradually, and this explains why the action of the gypsum lasts for several years. The advantage of manuring "fields with burned clay, and the fertility of ferruginous soils, which have been considered as facts so incomprehensible, may be explained in an equally simple manner. They have been ascribed to the great attraction for water, exerted by dry clay and ferruginous earth ; but common dry arable land possesses this property in as great a degree : and besides, what influence can be ascribed to a hundred pounds of water spread over an acre of land, in a condition in v/hich it cannot be serviceable either by the roots or leaves ? The true case is this : The oxides of iron and alumina are dis- tinguished from all other metallic oxides by their power of forming solid compounds with ammonia. The precipitates obtained by the addition of ammonia to salts of alu- * The urine of the horse contains, according to Fourcroy and "Vauquelin, in 1000 parts, Urea 7 parts. Hippurate of soda . . 24 " Salts and water . . 979 " 1000 parts. t Boussingault, Ann. de Ch. et de Phys. t. Ixiii. page 243. mina or iron are true salts, in wnich the ammonia is contained as a base. Minerals containing alumina or oxide of iron also possess, in an eminent degree, the remark- able property of attracting ammonia from the atmosphere and of retaining it. Vau- quelin, whilst engaged in the trial of a crimi- nal case, discovered that all rust of iron contains a certain quantity of ammonia. Chevalier afterwards found that ammonia is a constituent of all minerals containing iron ; that even hematite, a mineral which is not at all porous, contains one per cent, of it. Bouis showed also, that the peculiar odour observed on moistening minerals con- taining alumina, is partly owing to their ex- haling ammonia. Indeed, gypsum and some varieties of alumina, pipe-clay for ex- ample, emit so much ammonia, when mois- tened with caustic potash, that even after they had been exposed for two days, red- dened litmus paper held over them becomes blue. Soils, therefore, which contain ox- ides of iron, and burned clay, must absorb ammonia, an action which is favoured by their porous condition ; they further prevent the escape of the ammonia once absorbed by their chemical properties. Such soils, in fact, act precisely as a mineral acid would do, if extensively spread over their surface ; with this difference, that the acid would pe- netrate the ground, enter into combination with lime, alumina, and other bases, and thus lose, in a few hours, its properly of absorbing ammonia from the atmosphere. The addition of burned clay to soils has also a secondary influence; it renders the soil porous, and, therefore, more permeable to air and moisture. The ammonia absorbed by the clay or fer- ruginous oxides is separated by every shower of rain, and conveyed in solution to the soil. Powdered charcoal possesses a similar ac- tion, but surpasses all other substances in the power which it possesses of condensing ammonia within its pores, particularly when it has been previously heated to redness. Charcoal absorbs 90 times its volume of am- moniacal gas, which may be again separated by simply moistening it with water. (De Saussure.) Decayed wood approaches very nearly to charcoal in this power ; decayed oak wood absorbs 72 times its volume, after having been completely dried under the air- pump. We have here an easy and satisfac- tory means of explaining still further the pro- perties of humus, or wood in a decaying state. It is not only a slow and constant source of carbonic acid, but it is also a means by which the necessary nitrogen is conveyed to plants. Nitrogen is found in lichens, which grow on basaltic rocks. Our fields produce more of it than we have given them as manure, and it exists in all kinds of soils and mine- rals which were never in contact with or- ganic substances. The nitrogen in these cases could only have been extracted from the atmosphere. 36 AGRICULTURAL CHEMISTRY. We find this nitrogen in the atmosphere, in rain water, and in all kinds of soils, in the form of ammonia, as a product of the decay and putrefaction of preceding genera- tions of animals and vegetables. We find likewise that the proportion of azotised mat- ters in plants is augmented by giving them a larger supply of ammonia conveyed in the form of animal manure. No conclusion can then have a better foundation than this, that it is the ammonia of the atmosphere which furnishes nitrogen to plants. Carbonic acid, water and ammonia, con- tain the elements necessary for the support of animals and vegetables. The same sub- stances are the ultimate products of the chemical processes of decay and putrefac- tion. All the innumerable products of vi- tality resume, after death, the original form from which they sprung. And thus death the complete dissolution of an existing generation becomes the source of life for a new one. CHAPTER VI. OF THE INORGANIC CONSTITUENTS OP PLANTS. CARBONIC acid, water and ammonia, are necessary for the existence of plants, be- cause they contain the elements from which their organs are formed; but other sub- stances are likewise requisite for the forma- tion of certain organs destined for special functions peculiar to each family of plants. Plants obtain these subtances from inorganic nature. In the ashes left afler the incinera- tion of plants, the same substances are found, although in a changed condition. Although the vital principle exercises a great power over chemical forces, yet it does so only by directing the way in which they are to act, and not by changing the laws to which they are subject. Hence when the chemical forces are employed in the processes of vegetable nutrition, they must produce the same results which are observed in ordinary chemical phenomena. The inorganic matter contained in plants must, therefore, be subordinate to the laws which regulate its combinations in common chemical processes. The most important division of inorganic substances is that of acids and alkalies. Both of these have a tendency to unite together, and form, neutral compounds, which are termed salts. According to the doctrine of equivalents, these combinations are always effected in definite proportions, that is to say, one equivalent of an acid always unites with one or two equivalents of abase, what- ever that base may be. Thus 501-17 parts by weight of sulphuric acid unite with 1 eq. of potash, and form one eq. of sulphate of potash ; the same quantity unites with 1 eq. of soda, and produces sulphate of soda From this fact follows the rule that th quantity, which an acid requires of an alkali for its saturation, may be represented by a very simple number. It is perfectly necessary to form a proper conception of what chemists denominate the "capacity for saturation of an acid," before we are able to form a correct idea of the functions performed in plants, by their inorganic constituents. The power of a base to neutralize an acid does not depend upon the quantity of radical which it con- tains, but altogether upon the quantity of its oxygen. Thus protoxide of iron contains 1 eq. of oxygen, and unites with 1 eq. of sulphuric acid in forming a neutral salt; but peroxide of iron contains 3 eq. of oxygen, and requires 3 eq. of the same acid for its neutralization. Hence when a given weight of an acid is neutralized by different bases, the quantity of oxygen contained in these bases must be the same as is exhibited by the following scale : 501'17 parts of Sulphuric Acid neutralize 258 35 Magnesia Oxygen= 100 647-29 Strontia " =100 1451-61 Oxide of Silver " =100 956-8 Barytes " =100 It follows from the law of equivalents., that the quantity of oxygen in a base must stand in a simple relation to the quantity of oxygen in an acid which unites with it. By this is meant, that the quantities in both cases must either be equal or multiples of each other; for the doctrine of equivalents denies the possibility of their uniting in fractional parts. This will be rendered obvious by a consideration of the two following exam- ples : 100 parts of Cyanic Acid contain 23'2G oxy- gen=l. 100 parts of Cyanic Acid saturate 137'21 parts of potash, which contain 23'26 oxygen =1. 100 parts of Nitric Acid contain 73'85 oxygen = 5. 100 parts of Nitric Acid saturate 214'40 parts of oxide of silver, which contain 14'77 oxygen = 1. In the first of these cases, the relation of the oxygen of the base to that of the acid is as 1:1 ; in the second, as 1:5. The capacity for saturation of each acid, is, therefore, the constant quantity of oxygen necessary to neutralize 190 parts of it. Many of the inorganic constituents vary according to the soil in which the plants grow, but a certain number of them are in- dispensable to their developement. All sub- stances in solution in a soil are absorbed by the roots of plants, exactly as a sponge im- bibes a liquid, and all that it contains, with- out selection. The substances thus con- veyed to plants are retained in greater or less quantity, or are entirely separated when not suited for assimilation. Phosphate of magnesia in combination with ammonia is an invariable constituent of the seeds of all kinds of grasses. ^ It is contained in the outer horny husk, and is introduced into bread along with the flour, CONSTITUENTS OF PLANTS. 37 and also into beer. The bran of flour con- tains the greatest quantity of it. It is this sail which forms large crystalline concre- tions, often amounting to several pounds in weight, in the cfcciun of horses belonging to millers; and when ammonia is mixed with beer, the same salt separates as a white precipitate. Most plants, perhaps all of them, contain organic acids of very different composition and properties, all of which are in combi- nation with bases, such as potash, soda, lime, or magnesia. These bases evidently regulate the formation of the acids, for the diminution of the one is followed by a de- crease of the other: thus in the grape, for example, the quantity of potash contained in its juice is less when it is ripe than when unripe ; and the acids, under the same circumstances, are found to vary in a similar manner. Such constituents exist in small quantity in those parts of a plant in which the process of assimilation is most active, as in the mass of woody fibre; and their quantity is greater in those organs whose office it is to prepare substances con- veyed to them for assimilation by other parts. The leaves contain more inorganic matters than the branches, and the branches more than the stem. The potato plant con- tains more potash before blossoming than after it. The acids found in the different families of plants are of various kinds; it cannot be supposed that their presence and peculiari- ties are the result of accident. The fumaric and oxalic acids in the liverwort, the kinovic acid in the China nova, the rocellic acid in the Rocdla iinctoria, the tartaric acid in grapes, and the numerous other organic acids, must serve some end in vegetable life. But if these acids constantly exist in vege- tables, and are necessary to their life, which is incontestable, it is equally certain that some alkaline base is also indispensable, in order to enter into combination with the acids which are always found in the state of salts. All plants yield by incineration ashes containing carbonic acid ; all therefore must contain salts of an organic acid.* Now, as we know the capacity of satura- tion of organic acids to be unchanging, it follows that the quantity of the bases united with them cannot vary, and for this reason the latter substances ought to be considered with the strictest attention both by the agri- culturist and physiologist. We have no reason to believe that a plant in a condition of free and unimpeded growth produces more of its peculiar acids than it requires for its own existence; hence, a plant, on whatever soil it grows, must con- tain an invariable quantity of alkaline bases. Culture alone will be able to cause a devia- tion. * Salts of organic acids yield carbonates on in- cineration, if they contain either alkaline or earthy In order to understand this subject clearly, it will be necessary to bear in mind that any one of the alkaline bases may be substituted for another, the action of all being the same. Our conclusion is therefore by no means en- dangered by the existence ot a particular alkali in one plant, which may be absent in others of the same species. If this inference be correct, the absent alkali or earth must be supplied by one similar in its mode of ac- tion, or in other words, by an equivalent of another base. The number of equivalents of these various bases which may be com- bined with a certain portion of acid must necessarily be the same, and therefore the amount of oxygen contained in them must remain unchanged under all circumstances and on whatever soil they grow. Of course, this argument refers only to those alkaline bases which in the forni of organic salts form constituents of the plants. Now, these salts are preserved in the ashes of plants as carbonates, the quantity of which can be easily ascertained. It has been distinctly shown, by the analy- sis of De Saussure and Berthier, that the nature of a soil exercises a decided influence on the quantity of the different metallic ox- ides contained in the plants which grow on it ; that magnesia, for example, was con- tained in the ashes of a pine-tree grown at Mont Breven, whilst it was absent from the ashes of a tree of the same species from Mont La Salle, and that even the proportion of lime and potash was very different. Hence it has been concluded, (errone- ously, I believe,) that the presence of bases exercises no particular influence upon the growth of plants : but even were this view- correct, it must be considered as a most re- markable accident that these same analyses furnish proof for the very opposite opinion. For although the composition of the ashes of these pine-trees were so very different, they contained, according to the analyses of De Saussure, an equal number of equiva- lents of metallic oxides ; or, what is the same thing, the quantity of oxygen contained in all the bases was in both cases the same. 100 parts of the ashes of the pine-tree from Mont Breven contained Carbonate of Potash Lime " Magnesia 3-60 46-34 6-77 Sum of the carbonates 56'71 Quantity of oxygen in the Potash - 41 " " " Magnesia 1'27 Sum of the oxygen in the bases 9'01 100 parts of the ashes of the pine from Mont La Salle contained* * According to the experiments of Saussure, 1000 parts of the wood of the pine from Mont Brevon gave 11 '87 parts of ashes ; the same quan- tity of wood from Mont La Salle yielded 11 '28 parts. From this we might conclude that the two pines, although brought up in different soils, yet contained the'same quantity of -'inorganic elements. 38 AGRICULTURAL CHEMISTRY. Carbonate of Potash 7'36 Lime 5M9 Magnesia 0000 Sum of the carbonates 58 '55 Quantity of oxygen in the Potash 0'85 " " Lime 8'10 Sum of the oxygen in the bases 8 '95 The numbers 9O1 and 8*95 resemble each other as nearly as could be expected even in analyses made for the very purpose ol ascertaining the fact above demonstrated which the analyst in this case had not in view. Let us now compare Berthier's analyses of the ashes of two fir-trees,, one of which grew in Norway, the other in Allevard (de- partement de Plsere). One contained 50, the other 25 per cent, of soluble salts. A greater difference in the proportion of the alkaline bases could scarcely exist between two to- tally different plants, and yet even here the quantity of oxygen in the bases of both was. the same. 100 parts of the ashes of firwood from Allevard contained, according to Berthier, (Ann. de Chim. et de Phys. t. xxxii. p. 248,) Potash & Soda 16'8 in which 3'42 must be oxygen. Lime . 29'5 " 8.20 " Magnesia 3 '2 49.5 1.20 12-82 Only part of the potash and soda in these ashes was in combination with organic acids ; the remainder was in the form of sulphates, phosphates, and chlorides. One hundred parts of the ashes contain 3-1 sul- Shuric acid, 4-2 phosphoric acid, and 0-3 ydrochloric acid, which together neutralize a quantity of base containing 1-20 oxygen. This number therefore must be substracted from 12-82. The remainder 11-62 indicates the quantity of oxygen in the alkaline bases, combined with organic acids in the firwood of Allevard. The firwood of Norway contained in 100 parts, * Potash S 9 da Lime . 14*1 of which 2'4 would be oxygen. 207 " 5-3 . 123 " 3-45 " " 4-35 " 1-69 " " 51-45 12-84 And if the quantity of oxygen of the bases in combination with sulphuric and phosphoric acid, viz. 1-37, be again sub- stracted from 12-84, 11-47 parts remain as the amount of oxygen contained in the bases which were in combination with organic acids. * This calculation is exact only in the case where the quantity of ashes is equal in weight for a given quantity of wood ; the difference cannot, however, be admitted to be so great as to change sensibly the above proportions- Berthier has not mentioned the proportion of ashes contained in the wood. These remarkable approximations cannot be accidental ; and if further examinations confirm them in other kinds of plants, no other explanation than that already given can be adopted. It is not known in what form silica, man ganese, and oxide of iron, are contained iu plants; but we are certain that potash, soda, and magnesia, can be extracted from all parts of their structure in the form of salts of organic acids. The same is the case with lime, when not present as insoluble oxalate of lime. It must here be remembered, that in plants yielding oxalic acid, the acid and potash never exist in the form of a neutral or quadruple salt, but always as a double acid salt, on whatever soil they may grow. The potash in grapes also is more frequently found as an acid salt, viz. cream of tartar, (bitartrate of potash,) than in the form of a neutral compound. As these acids and bases are never absent from plants, and as even the form in which they present them- selves is not subject to change, it may be affirmed that they exercise an important in- fluence on the developement of the fruits and seeds, and also on many other functions of he nature of which we are at present igno- rant. The quantity of alkaline bases existing in a plant also depends evidently on this cir- cumstance of their existing only in the form of acid salts, for the capacity of saturation of an acid is constant ; and when we see oxalate of lime in the lichens occupying the Dlace of woody fibre which is absent, we must regard it as certain that the soluble or- ganic salts are destined to fulfil equally im- portant though different functions, so much so that we could not conceive the complete developement of a plant without their pre- sence, that is, without the presence of their acids, and consequently of their bases. From these considerations we must per- ceive, that exact and trustworthy examina- ions of the ashes of plants of the same kind growing upon different soils would be of the greatest importance to vegetable physiology' and would decide whether the lacts above nentioned are the results of an unchanging aw for each family of plants, and whether an invariable number can be found to ex- cess the quantity of oxygen which each species of plant contains in the bases united h organic acids. In all probability such nquirieswill lead to most important results; 'or it is clear that if the production of a cer- ain unchanging quantity of an organic acid s required by the peculiar nature of the rgans of a plant, and is necessary to its ex- stence, then potash or lime must be taken up by it in order to form salts with this acid j hat if these do not exist in sufficient quan- ity in the s^oil, other bases must supply their lace; and that the progress of a plant must )e wholly arrested when none are present. Seeds f the Salsola Kali, when sown in ommon garden soil, produce a plant con- aining both potash and soda ; while the CONSTITUENTS OF PLANTS. 39 plants grown from ihe seeds of this contain only salts of potash, with mere traces of muriate of soda. (Cadet.) The examples cited above, in which the quantity of oxygen contained in the bases was shown to be the same, lead us to the legitimate conclusion that the developement of certain plants is not retarded by the sub- stitution of the bases contained in them. But it was by no means inferred that any one base could replace all the others which are found in a plant in its normal condition. On the contrary, it is known that certain bases are indispensable for the growth of a plant, and these could not be substituted without injuring its developement. Our in- ference has been drawn from certain plants, which can bear without injury this substitu- tion ; and it can only be extended to those plants which are in the same condition. It will be shown afterwards that corn or vines can only thrive on soils containing potash, and that this alkali is perfectly indispensable to their growth. Experiments have not been sufficiently multiplied so as to enable us to point out in what plants potash or soda may be replaced by lime or magnesia; we are only warranted in affirming that such substitutions are in many cases common. The ashes of various kinds of plants contain very different quantities of alkaline bases, such as potash, soda, lime, or magnesia. When lime exists in the ashes in large pro- portion, the quantity of magnesia is dimi- nished, and in like manner according as the latter increases the lime or potash decreases. In many kinds of ashes not a trace of mag- nesia can be detected. The existence of vegetable alkalies in com- bination with organic acids gives great weight to the opinion that alkaline bases in general are connected with the developement of plants. If potatoes are grown where they are not supplied with earth, the magazine of inor- ganic bases, (in cellars, for example,) a true alkali, called Solanin, of very poisonous nature, is formed in the sprouts which ex- tend towards the light, while not the smallest trace of such a substance can be discovered m the roots, herbs, blossoms, or fruits of potatoes grown in fields. (Otto.) In all the species of the Cinchona, kinic acid is found; but the quantity of quinina, cinchonina, and hme, which they contain is most variable. From the fixed bases in the products of in- cineration, however, we may estimate pretty accurately the quantity of the peculiar or- ganic bases. A maximum of the first cor- responds to a minimum of the latter, as must necessarily be the case if they mutually replace one another according to their equi- valents. We know that different kinds of opium contain meconic acid in combination with very different quantities of narcotina, morphia, codeia, &c., the quantity of one of these alkaloids diminishingon the increase of the others. Thus the smallest quantity of morphia is accompanied by a maximum of narcotina. Not a trace of meconic acid* can be discovered in many kinds of opium, but there is not on this account an absence of acid, for the meconic is here replaced by sulphuric acid. Here, also, we have an ex- ample of what has been before stated, for in those kinds of opium where both these acids exist, they are always found to bear a cer- tain relative proportion to one another. At- tention to these facts must be very important in the selection of soils destined for the cul- tivation of plants which yield the vegetable alkaloids. Now if it be found, as appears to be the case in the juice of poppies, that an organic acid may be replaced by an inorganic, with- out impeding the growth of a plant, we must admit the probability of this substitution taking place in a much higher degree in the case of the inorganic bases. When roots find their more appropriate base in sufficient quantity, they will take up less of another. These phenomena do not show themselves so frequently in cultivated plants, because they are subjected' to special external condi- tions for the purpose of the production of particular constituents or particular organs. When the soil, in which a white hyacinth is growing in a state of blossom, is sprinkled with the juice of the Phytolacca decandra, the white blossoms assume in one or two hours a red colour, which again disappears after a few days under the influence of sun- shine, and they become white and colourless as before.f The juice in this case evidently enters into all parts of the plant, without being at all changed in its chemical nature, or without its presence m being apparently either necessary or injurious. But this con- dition is not permanent, and when the blos- soms have again become colourless, none of the colouring matter remains j and if it should occur that any of its elements were adapted for the purposes of nutrition of the plant, then these alone would be retained, whilst the rest would be excreted in an al- tered form by the roots. Exactly the same thing must happen when we sprinkle a plant with a solution of chloride of potassium, nitre, or nitrate of strontia; they will enter into the different parts of the plant, just as the coloured juice mentioned above, and will be found in its ashes if it should be burnt at this period. Their presence is merely accidental ; but no conclusion can be hence deduced against the necessity of the presence of other bases in plants. The experiments of Macaire- Princep have shown, that plants made to vegetate with their roots in a weak solution of acetate of lead, and then in rain water, * Robiquet did not obtain a trace of meconate of lime from 300 Ibs. of opium, whilst in other kinds the quantity was very considerable. Ann. de Chim. liii. p. 425. t Biot, in the Comptes rendus des Seances de ['Academic des Science^, a Paris, ler Semestre, 1837, p.'12. 40 AGRICULTURAL CHEMISTRY. yield to the latter all the salt of lead which they had previously absorbed. They return, therefore, to the soil all matters which are unnecessary to their existence. Again, when a plant, freely exposed to the atmosphere, rain, and sunshine, is sprinkled with a solu- tion of nitrate of strontia, the salt is ab- sorbed, but it is again separated by the roots and removed farther from them by every shower of rain, which moistens the soil, so that at last not a trace of it is to be found in the plant. Let us consider the composition of the ashes of two fir-trees as analysed by an acute and most accurate chemist. One of these grew in Norway, on a soil the constituents of which never changed, but to which solu- ble salts, and particularly common salt, were conveyed in great quantity by rain-water. How did it happen that its ashes contained no appreciable trace of salt, although we are certain that its roots must have absorbed it after every shower? We can explain the absence of salt in this case by means of the direct and positive observations referred to, which have shown that plants have the power of returning to the soil all substances unnecessary to their existence; and the conclusion to which all the foregoing facts lead us, when their real value and bearing are apprehended, is that the alkaline bases existing in the ashes of plants must be* necessary to their growth, since if this were not the case they would not be retained. The perfect developement of a plant, ac- cording to this view, is dependent on the presence of alkalies or alkaline earths ; for when these substances are totally wanting its growth will be arrested, and when they are only deficient it must be impeded. In order to apply these remarks, let us compare two kinds of trees, the wood of which contains unequal quantities of alka- line bases, and we shall find that one of these grows luxuriantly in several soils upon which the others are scarcely able to vege- tate. For example, 10,000 parts of oak wood yield 250 parts of ashes, the same quantity of fir wood only 83, of linden wood 500, of rye 440, and of the herb of the po- tato plant 1500 parts.* Firs and pines find a sufficient quantity of alkalies in granitic and barren sandy soils in which oaks will not grow ; and wheat thrives in soils favourable for the linden tree, because the bases which are necessary to bring it to complete maturity, exist there in sufficient quantity. The accuracy of these conclusions, so highly important to agriculture and to the cultivation of forests, can be proved by the most evident facts. All kinds of grasses, the Equisetacem, for example, contain in the outer parts of their leaves and stalk a large quantity of silicic acid and potash in the form of acid silicate * Berthier, Annales de Chimie et de Physique, f. xxx. p. 248. of potash. The proportion of this salt does not vary perceptibly in the soil of corn-fields, because it is again conveyed to them as ma- nure in the form of putrefying straw. But this is not the case in a meadow, and hence we never find a luxuriant crop of grass* on sandy and calcareous soils, which contain little potash, evidently because one of the constituents indispensable to the growth of the plants is wanting. Soils formed from basalt, grauwacke, and porphyry are, catena paribus, the best for meadow land, on ac- count of the quantity of potash which enters into their composition. The potash ab- stracted by the plants is restored during the annual irrigation. The potash contained in the soil itself is inexhaustible in comparison with the quantity removed by plants. But when we increase the crop of grass in a meadow by means of gypsum, we remove a greater quantity of potash with the hay than can under the same circumstances be restored. Hence it happens that, after the lapse of several years, trie crops of grass on the meadows manured with gypsum dimi- nish, owing to the deficiency of potash. Bat if the meadow be strewed from time to time with wood-ashes, even with the lixiviated ashes which have been used by soap-boilers, (in Germany much soap is made from the ashes of wood,) then the grass thrives as luxuriantly as before. The ashes are only a means of restoring the potash. A harvest of grain is obtained every thirty or forty years from the soil of the Luneburg heath, by strewing it with the ashes of the heath plants (Erica vul^aris) which grow on it. These plants during the long period just mentioned collect the potash and soda, which are conveyed to them by rain-water; and it is by means of these alkalies that oats, barley, and rye, to which they are indis- pensable, are enabled to grow on this sandy heath. The woodcutters in the vicinity of Heidel - berg have the privilege of cultivating the soil for their own use, after felling the trees used for making tan. Before sowing the land thus obtained, the branches, roots, and leaves, are in every case burned, and the ashes used as a manure, which is found to be quite indispensable for the growth of the grain. The soil itself upon which the oats grow in this district consists of sandstone ; and although the trees find in it a quantity of alkaline earths sufficient for their own sustenance, yet in its ordinary condition it is incapable of producing grain. The most decisive proof of the use of strong manure was obtained at Bingen (a town on the Rhine,) where the produce and deve- lopement of vines were highly increased by * It would be of importance to examine what alkalies are contained in the ashes of the sea-shore plants which grow in the humid hollows of downs, and especially in those of the millet-grass. If potash is not found in them, it must certainly b replaced by soda as in the Salsola, or by lime as in the Plumbaginece. CONSTITUENTS OP PLANTS. 41 manuring them with such substances as shav- ings of horn, &c. ; but after some years the formation of the wood and leaves decreased ;o the great loss of the possessor, to such a degree that he has long had cause to regret his departure from the usual methods. By the manure employed by him, the vines had been too much hastened in' their growth ; in two or three years they had exhausted the potash in the formation of their fruit, leaves, and wood, so that none remained for the fu- ture crops, his manure not having contained any potash. There are vineyards on the Rhine the plants of which are a hundred years old, and all of these have been cultivated by manuring them with a cow-dung, a manure containing a large proportion of potash, although very little nitrogen. All the potash, in fact, which is contained in the food con- sumed by a cow is again immediately dis- charged in its excrements. The experience of a proprietor of land in the vicinity of Gottingen offers a most re- markable example of the incapability of a soil to produce wheat or grasses in general, when it fails in any one of the materials ne- cessary to their growth. In order to obtain potash, he planted his whole land with wormwood, the ashes of which are well known to contain a large proportion of the carbonate of that alkali. The consequence was, that he rendered his land quite incapa- ble of bearing grain for many years, in con- sequence of having entirely deprived the soil of its potash. The leaves and small branches of trees contain the most potash; and the quantity of them which is annually taken from a wood for the purpose of being employed as litter,* contain more of that alkali than all the old wood which is cut down. The bark and foliage of oaks, for example, con- tain from 6 to 9 per cent, of this alkali ; the needles of firs and pines, 8 per cent. With every 2650 Ibs. of firwood which are yearly removed from an acre of forest, only from 0-114 to 0-53 Ibs. of alkalies are abstracted from the soil, calculating the ashes at 0-83 per cent. The moss, however, which covers the ground, and of which the ashes are known to contain so much alkali, continues uninterrupted in its growth, and retains that potash on the surface, which would otherwise so easily penetrate with the rain through the sandy soil. By its de- cay, an abundant provision of alkalies is supplied to the roots of the trees, and a fresh supply is rendered unnecessary. * [This refers to a custom some time since very prevalent in Germany although now discon- tinued. The leaves and small twigs of trees were gleaned from the forests by poor people, for the purpose of being used as litter for their cattle. The trees, however, were found to suffer so much in consequence, that their removal is strictly pro- hibited. The cause of the injury was that stated the text. ED.] 6 The supposition of alkalies, metallic ox- ides, or inorganic matter in general, being produced by plants, is entirely refuted by these well-authenticated facts. It is thought very remarkable, that those plants of the grass tribe, the seeds of which furnish food for man, follow him like the domestic animals. But saline plants seek the sea-shore or saline springs, and the Chenopodium the dunghill from similar causes. Saline plants require common salt, and the plants which grow only on dung- hills need ammonia and nitrates, and they are attracted whither these can be found, just as the dung-fly is to animal excrements. So likewise none of our corn-plants can bear perfect seeds, that is, seeds yielding flour, without a large supply of phosphate of magnesia and ammonia, substances which, they require for their maturity. And hence, these plants grow only in a soil where these three constituents are found combined, and no soil is richer in them than those where men and animals dwell together; where the urine and excrements of these are found corn-plants appear, because their seeds can- not attain maturity unless supplied with the constituents of those matters. When we find sea-plants near our salt- works, several hundred miles distant from the sea, we know that their seeds have been carried there in a very natural manner, namely, by wind or Birds, which have spread them over the whole surface of the earth, although they grow only in those places in which they find the conditions essential to their life. Numerous small fish, of not more than two inches in length (Gasterosteusaculeatusi) are found in the salt-pans of the graduating house at Nidda (a village in Hesse Darm- stadt.) No living animal is found in the salt-pans of Neuheim, situated about 18 miles from Nidda; but the water there con- tains so much carbonic acid and lime, that the walls of the graduating house are covered with stalactites. Hence the eggs conveyed to this place by birds do not find the condi- tions necessary for their developement, which they found in the former place.* * The itch-insect (Acarus Scabiei) is considered by Burdach as the production of a morbid condi- tion, so likewise lice in children ; the original generation of the fresh-water muscle (mytilus) in fish-ponds, of sea-plants in the vicinity of salt- works, of nettles and grasses, of fish in pools of rain, of trout in mountain streams, &c., is ac- cording to the same natural philosopher not im- possible. A soil consisting of crumbled rocks, decayed vegetables, rain and salt water, &c., is here supposed to possess the power of generating shell-fish, trout, and saltwort (salicornia.') All inquiry is arrested by such opinions, when propa- gated by a teacher who enjoys a merited reputa- tion, obtained by knowledge and hard labour. These subjects, however, have hitherto met with the most superficial observation, although they well ment strict investigation. The dark, the secret, the mysterious, the enigmatic, is, in fact, too seducing for the youthful and philosophic D 2 42 AGRICULTURAL CHEMISTRY. How much more wonderful and inexpli- cable does it appear, that bodies which re- mained fixed in the strong heat of a fire, have under certain conditions the property of volatilizing and, at ordinary temperatures, of passing into a state, of which we cannot say whether they have really assumed the form of a gas or are dissolved in one ! Steam f/r vapours in general have a very singular influence in causing the volatilization of such bodies, that is, of causing them to as- sume the gaseous form. A liquid during evaporation communicates the power of as- suming the same state in a greater or less degree to all substances dissolved in it, although they do not of themselves possess that property. Boracic acid is a substance which is com- pletely fixed in the fire 5 it suffers no change of weight appreciable by the most delicate balance, when exposed to a white heat, and, therefore, it is not volatile. Yet its solution in water cannot be evaporated by the gen- tlest heat, without the escape of a sensible quantity of the acid with the steam. Hence it is that a loss is always experienced in the analysis of minerals containing this acid, when liquids in which it is dissolved are evaporated. The quantity of boracic aoid which escapes with a cubic foot of steam, at the temperature of boiling water, cannot be detected by our most sensible re-agents ; and nevertheless the many hundred tons annually brought from Italy as an article of commerce, are procured by the uninter- rupted accumulation of this apparently in- appreciable quantity. The hot steam which issues from the interior of the earth is al- lowed to pass through cold water in the lagoons of Castel Nupva and Cherchiago j in this way the boracic acid is gradually accu- mulated, till at last it may be obtained in crystals by the evaporation of the water. It is evident, from the temperature of the steam, that it must have come out of depths in which human beings and animals never could have lived, and yet it is very remarka- ble and highly important that ammonia is never absent from it. In the large works in Liverpool, where natural boracic acid is converted into borax, many hundred pounds of sulphate of ammonia are obtained at the same time. This ammonia has not been produced by the animal organism, it existed before the creation of human beings; it is a part, a primary constituent, of the globe itself. The experiments instituted under Lavoi- sier's guidance by the Direction des Poudres et Salpetres, have proved that during the evaporation of the saltpetre ley, the salt volatilizes with the water, and causes a loss which could not before be explained. It is known also, that in sea storms, leaves of mind, which would penetrate the deepest depths of nature, without the assistance of the shaft or ladder of the miner. This is poetry, but not sober philosophical inquiry. plants in the direction of the wind are covered with crystals of salt, even at the distance of from 20 to 30 miles from the sea. But it does not require a storm to cause the volatilization of the salt, for the air hanging over the sea always contains enough of this substance to make a solution of nitrate of silver turbid, and every breeze must carry this away. Now, as thousands of tons of sea water annually evaporate into the atmosphere, a corresponding quantity of the salts dissolved in it, viz. of common salt, chloride of potassium, magnesia, and the remaining constituents of the sea water, will be conveyed by wind to the land. This volatilization is a source of con- siderable loss in salt works, especially where the proportion of salt in the water is not large. This has been completely proved at the salt works of Nauheim, by the very intelligent director of that establishment, M. Wilhelmi. He hung a plate of glass be- tween two evaporating houses, which were about 1200 paces distant from each other, and found in the morning, after the drying of the dew, that the glass was covered with crystals of salt on one or the other side, ac- cording to the direction of the wind. By the continual evaporation of the sea, its salts* are spread over the whole surface of the earth ; and being subsequently car- ried down by the rain, furnish to the vegeta- tion those salts necessary to its existence. This is the origin of the salts found in the ashes of plants, in those cases where the soil could not have yielded them. In a comprehensive view of the phe- nomena of nature, we have no scale for that which we are accustomed to name, small or great; all our ideas are proportioned to what we see around us, but how insig- nificant are they in comparison with the whole mass of the globe! that which is scarcely observable in a confined district appears inconceivably large when regarded in its extension through unlimited space. The atmosphere contains only a thousandth part of its weight of carbonic acid ; and yet small as this proportion appears, it is quite * According to Marcet, sea- water contains in 1000 parts, 26-660 Chloride of Sodium. 4-660 Sulphate of Soda. 1-232 Chloride of Potassium. 5'152 Chloride of Magnesium. 0-153 Sulphate of Lime. According to M'Clemm, the water of the North Sea contains in 1000 parts, 24-84 Chloride of Sodium. 2'42 Chloride of Magnesium. 2'06 Sulphate of Magnesia. T25 Chloride of Potassium. 1-20 Sulphate of Lime. In addition to these constituents, it also con- tains inappreciable quantities of carbonate of lime, magnesia, iron, manganese, phosphate of lime, iodides and bromides, silica, sulphuretted hy- drogen, and organic matter, together with am- monia and carbonic acid. (Liebig's Annalen der Ckemie, Bd. xxxvii. s. 3.) THE ART OF CULTURE. 43 sufficient to supply the whole of the present generation of living beings with carbon for a thousand years, even if it were not re- newed. Sea-water contains -j-oiinF f i* 8 weight of carbonate of lime; and this quan- tity, although scarcely appreciable in a pound, is the source from which myriads of marine mollusca and corals are supplied with materials for their habitations. Whilst the air contains only from 4 to 6 ten-thousandth parts of its volume of car- bonic acid, sea-water contains 100 times more, (10,000 volumes of sea-water contain 620 volumes of carbonic acid Laurent, Bouillon, Lagrange.) Ammonia* is also found in this water, so that the same condi- tions which sustain living beings on the land are combined in this medium, in which a whole world of other plants and animals exist. The roots of plants are constantly en- gaged in collecting from the rain those alkalies which formed part of the sea-water, and also those of the water of springs, which penetrates the soil. Without alkalies and alkaline bases most plants could not exist, and without plants the alkalies would disappear gradually from the surface of the earth. When it is considered, that sea-water con- tains less than one-millionth of its own weight of iodine, and that all combinations of iodine with the metallic bases of alkalies are highly soluble in water, some provision must necessarily be supposed to exist in the organization of sea-weed and the different kinds of Fuci, by which they are enabled during their life to extract iodine in the form of a soluble salt from sea-water, and to assimilate it in such a manner, that it is not again restored to the surrounding me- dium. These plants are collectors of iodine, just as land plants are of alkalies ; and they yield us this element, in quantities such as we could not otherwise obtain from the water without the evaporation of whole seas. We take it for granted that the sea-plants require metallic iodides for their growth, and that their existence is dependent on the presence of those substances. With equal justice, then, we conclude, that the alkalies and alkaline earths, always found in the ashes of land-plants, are likewise necessary for their developement. CHAPTER VII. THE ART OP CULTURE. THE conditions necessary for the life of all vegetables have been considered in the * When the solid saline residue obtained by the evaporation of sea- water is heated in a retort to redness, a sublimate of sal-ammoniac is obtained. -MARCET. preceding part of the work. Carbonic acid, ammonia, and water yield elements for all the organs of plants. Certain inorganic substances salts and metallic oxides serve peculiar functions in their organism, ana many of them must be viewed as essential constituents of particular parts. The atmosphere and the soil offer the same kind of nourishment to the leaves and roots. The former contains a comparatively inex- haustible supply of carbonic acid and am- monia ; the latter, by means of its humus, generates constantly fresh carbonic acid, whilst, during the winter, rain and snow in- troduce into the soil a quantity of ammonia, sufficient for the developement of the leaves and blossoms. f The complete, or it may be said, the' abso- lute insolubility in cold water of vegetable matter in progress of decay, (humus,) ap- pears on closer consideration to be a most wise arrangement of nature. For if humus possessed even a smaller degree of solubility than that ascribed to the substance called hu- mic acid, it must be dissolved by rain-water. Thus, the yearly irrigation of meadows, which last for several weeks, would remove a great part of it from the ground, and a heavy and continued rain would impoverish the soil. But it is soluble only when com- bined with oxygen ; it can be taken up by water, therefore, only as carbonic acid. When kept in a dry place, humus may be preserved for centuries; but when moist- ened with water, it converts the surrounding oxygen into carbonic acid. As soon as the action of the air ceases, that is, as soon as it is deprived of oxygen, the humus suffers no far- ther change. Its decay proceeds only when plants grow in the soil containing it; for they absorb by their roots the carbonic acid as it is formed. The soil receives again from living plants the carbonaceous matter it thus loses, so that the proportion of humus in it does not decrease. The stalactitic caverns in Franconia, and those in the vicinity of Baireuth, and Slreit- berg, lie beneath a fertile arable soil; the abundant decaying vegetables or humus in this soil, being acted on by moisture and air, constantly evolve carbonic acid, which is dis- solved by the rain. The rain-water thus impregnated permeates the porous lime- stone, which forms the walls and roofs of the caverns, and dissolves in its passage as much carbonate of lime as corresponds to the quantity of carbonic acid contained in it. Water and the excess of carbonic acid eva- porate from this solution when it has reached the interior of the caverns, and the limestone is deposited on the walls and roofs in crys- talline crusts of various forms. There are few spots on the earth where so many cir- cumstances favourable to the production of humate of lime are combined, if the humus actually existed in the soil in the form of humic acid. Decaying vegetable matter, water, and lime in solution, are brought to- gether, but the stalactites formed contain no 44 AGRICULTURAL CHEMISTRY. irace of vegetable matter, and no humic acid ; they are of a glistening white or yel- lowish colour, and in part transparent, like calcareous spar, and may be heated to red- ness without becoming black. The subterranean vaults in the old castles near the Rhine, the " Bergstrass," and Wetherau, are constructed of sandstone, granite, or basalt, and present appearances similar to the limestone caverns. The roofs of these vaults or cellars are covered exter- nally to the thickness of several feet with vegetable mould, which has been formed by the decay of plants. The rain falling upon them sinks through the earth, and dissolves the mortar by means of the carbonic acid derived from the mould ; and this solution evaporating in the interior of the vaults, covers them with small thin stalactites, which are quite free from humic acid. In such a filtering apparatus, built by the hand of nature, we have placed before us ex- periments which have been continued for a hundred or thousand years. Now, if water possessed the power of dissolving a hun- dredth thousandth part of its own weight of humic acid or humate of lime, and humic acid were present, we should find the inner surface of the roofs of these vaults and cav- erns covered with these substances ; but we cannot detect the smallest trace of them. There could scarcely be found a more clear and convincing proof of the absence of the humic acid of chemists in common vegeta- ble mould. The common view, which has been adopted respecting the modus operandi of humic acid, does not afford any explanation of the following phenomenon: A very small quantity of humic acid dissolved in water gives it a yellow or brown colour. Hence it would be supposed that a soil would be more fruitful in proportion as it was capable of giving this colour to water, that is, of yielding it humic acid. But it is very remarkable that plants do not thrive in such a soil, and that all manure must have lost this property before it can exercise a fa- vourable influence upon their vegetation. Water from barren peat soils and marshy meadows, upon which few plants flourish, contains much of this humic acid ; but all agriculturists and gardeners agree that the most suitable and best manure for plants is that which has completely lost the property of giving a colour to water. The soluble substance, which gives to water a brown colour, is the product of the putrefaction of all animal and vegetable matter; its formation is an evidence that there is not oxygen sufficient to begin, or at least to complete the decay. The brown so- lutions containing this substance are deco- lourised in the air by absorbing oxygen, and a black coaly matter precipitates the sub- stance named " coal of humus." Now if a soil were impregnated with this matter, the effect on the roots of plants would be the same as that of entirely depriving the soil of oxygen; plants would be as little able 'to grow in such ground as they would if hy- drated protoxide of iron were mixed with the soil. Indeed some barren soils have been found to owe their fertility to this very cause The sulphate of protoxide of iron (coppe ras,) which forms a constituent of these soils, possesses a powerful affinity for oxygen, and consequently prevents the absorption of that gas by the roots of plants in its vicinity.* All plants die in soils and water which con- tain no oxygen ; absence of air acts exactly in the same manner as an excess of carbonic acid. Stagnant water on a marshy soil ex- cludes air, but a renewal of water has the same effect as a renewal of air, because wa- ter contains it in solution. If the water is withdrawn from a marsh, free access is given to the air, and the marsh is changed into a fruitful meadow. In a soil to which the air has no access, or at most but very little, the remains of ani- mals and vegetables do not decay, for they can only do so when freely supplied with oxygen; but they undergo putrefaction, for which air is present in sufficient quantity. Putrefaction is known to be a most powerful deoxidising process, the influence of which extends to all surrounding bodies, even to the roots and the plants themselves. All substances from which oxygen can be ex- tracted yield it to putrefying bodies ; yellow oxide of iron passes into the state of black oxide, sulphate of iron into sulphuret of iron, &c. The frequent renewal of air by ploughing, and the preparation of the soil, especially its contact with alkaline metallic oxides, the ashes of brown coal, burnt lime or limestone, change the putrefaction of its organic con- stituents into a pure process of oxidation ; and from the moment at which all the or- ganic matter existing in a soil enters into a state of oxidation or decay, its fertility is in- creased. The oxygen is no longer employed for the conversion of the brown soluble mat- ter into the insoluble coal of humus, but serves for the formation of carbonic acid. This change takes place very slowly, and in. some instances the oxygen is completely ex- cluded by it; and whenever this happens, the soil loses its fertility. Thus, in the vicinity of Salzhausen (a village in Hesse Darmstadt, famed for its mineral springs, upon a meadow called Grunschwalheimer, unfruitful spots are seen here and there covered with a yellow grass. If a hole be bored from twenty to twenty-five feet deep in one of these spots, carbonic acid is emit- ted from it with such violence that the noise made by the escape of the gas may be dis- * The most obvious method of removing thia salt from soils in which it may be contained is to manure the land with lime. The lime unites with the sulphuric acid and liberates the protoxide of iron, which absorbs oxygen with much rapidity, and is converted into the peroxide of iron. Thia conversion is accelerated by giving free access te the air, that is. by loosening the soil. THE ART OF CULTURE}. 45 tmctly heard at the distance of several feet. Here the carbonic acid rising to the surface displaces completely all the air, and conse- quently all the oxygen, from the soil; and and without oxygen neither seeds nor roots can be developed; a plant will not vegetate in pure nitrogen or carbonic acid gas. Humus supplies young plants with nou- rishment by the roots, until their leaves are matured sufficiently to act as exterior organs of nutrition ; its quantity heightens the fer- tility of a soil by yielding more nourishment in this first period of growth, and conse- quently by increasing the number of organs of atmospheric nutrition. Those plants which receive their first food from the sub- stance of their seeds, such as bulbous plants, could completely dispense with humus ; its presence is useful only in so far as it in- creases and accelerates their developement, but it is not necessary indeed, an excess of it at the commencement of their growth is in a certain measure injurious. The amount of food which young plants can take from the atmosphere in the form of carbonic acid and ammonia is limited; they cannot assimilate more than the air contains. Now, if the quantity of their stems, leaves, and branches has been increased by the ex- cess of food yielded by the soil at the com- mencement of their developement, they will require for the completion of their growth, and for the formation of their blossoms and fruits, more nourishment from the air than it can afford, and consequently they will not reach maturity. In many cases the nourishment afforded by the air under these circumstances suffices only to complete the formation of the leaves, stems, and branches. The same result then ensues as when orna- mental plants are transplanted from the pots in which they have grown to larger ones, in which their roots are permitted to increase and multiply. All their nourishment is em- ployed for the increase of their roots and leaves ; they spring, as it is said, into an herb or weed, but do not blossom. When, on the contrary, we take away part of the branches, and of course their leaves with them, from dwarf trees, since we thus pre- vent the developement of new branches, an excess of nutriment is artificially procured for the trees, and is employed by them in the increase of the blossoms and enlargement of the fruit. It is to effect this purpose that vines are pruned. A new and peculiar process of vegetation ensues in all perennial plants, such as shrubs, fruit and forest trees, after the com- plete maturity of their fruit. The stem of annual plants at this period of their growth becomes woody, and their leaves change in colour. The leaves of trees and shrubs, on the contrary, remain in activity until the com- mencement of the winter. The formation of the layers of wood progresses, the wood becomes harder and more solid, but after August the leaves form no more wood; all the carbonic acid which the plants now ab- sorb is employed for the production of nu- tritive matter for the following year : instead of woody fibre, starch is formed, and is dif- fused through every part of the plant by the autumnal sap (seve d'Aout)* According to the observations of M. Heyer, the starch hus deposited in the body of the tree can be recognised in its known form by the aid of a good microscope. The barks of several as- pens and pine-treesf contain so much of this substance, that it can be extracted from them as from potatoes by trituration with water. It xists also in the roots and other parts of pe- rennial plants. A very early winter, or sudden change of temperature, prevents the forma- tion of this pro vision for the following year; the wood, as in the case of the vine-slock, does not ripen, and its growth is in the next year very limited. From the starch thus accumulated, sugar and gum are produced in the succeeding spring, while from the gum those constitu- ents of the leaves and young sprouts which, contain no nitrogen are in their turn formed. After potatoes have germinated, the quantity of starch in them is found diminished. The juice of the maple-tree ceases to be sweet from the loss of its sugar when its buds, blossoms, and leaves attain their maturity. The branch of a willow, which contains a large quantity of granules of starch in every part of its woody substance, puts forth both roots and leaves in pure distilled rain- water; but in proportion as it grows, the starch disappears, it being evidently ex- hausted for the formation of the roots and leaves. In the course of these experiments, M. Heyer made the interesting observation, that such branches when placed in snow water (which contains ammonia) produced roots three or four times longer than those which they formed in pure distilled water, and that this pure water remained clear, while the rain-water gradually acquired a yellow colour. Upon the blossoming of the sugar-cane, likewise, part of the sugar disappears ; and it has been ascertained, that the sugar does not accumulate in the beet-root until after the leaves are completely formed. Much attention has recently been drawn to the fact that the produce of potatoes may- be much increased by plucking off the blos- soms from the plants producing them, a result quite consistent with theory. This important observation has been completely confirmed by M. Zeller, the director of the Agricultural Society at Darmstadt. In the year 1839, two fields of the same size, lying side by side and manured in the same man- ner, were planted with potatoes. When the plants had flowered, the blossoms were re- * Hartig, in Erdmann und Schweigger-Seidels Journal, V. 217. 1335. .T It is well known that bread is made from the bark of pines in Sweden during famines. AGRICULTURAL CHEMISTRY. moved from those in one field, while those in the other field were left untouched. The former produced 47 bolls, the latter only 37 bolls. These well-authenticated observations re- move every doubt as to the part which sugar, starch, and gum play in the developement of plants ; and it ceases to be enigmatical, why these three substances exercise no influence on the growth or process of nutrition of a matured plant, when supplied to them as food. The accumulation of starch in plants during the autumn has been compared, al- though certainly erroneously, to the fatten- ing of hibernating animals before their winter sleep; but in these animals every vital func- tion, except the process of respiration, is suspended, and they only require, like a lamp slowly burning, a substance rich in carbon and hydrogen to support the pro- cess of combustion in the lungs. On their awaking from their torpor in the spring, the fat has disappeared, but has not served as nourishment. It has not caused the least increase in any part of their body, neither has it changed the quality of any of their organs. With nutrition, properly so called, the fat in these animals has not the least connexion. The annual plants form and collect their future nourishment in the same way as the perennial ; they store it in their seeds in the form of vegetable albumen, starch and gum, which are used by the germs for the forma- tion of their leaves and first radicle fibres. The proper nutrition of the plants, their in- crease in size, begins after these organs are formed. Every germ and every bud of a perennial plant is the engrafted embryo of a new indi- vidual, while the nutriment accumulated in the stem and roots, corresponds to the albu- men of the seeds. Nutritive matters are, correctly speaking, those substances which, when presented from without, are capable of sustaining the life and all the functions of an organism, by furnishing to the different parts of plants the materials for the production of their peculiar constituents. In animals, the blood is the source of the material of the muscles and nerves; by one of its component parts, the blood supports the process of respiration, by others, the peculiar vital functions; every part of the body is supplied with nourishment by it, but its own production is a special function, without which we could not conceive life to continue. If we destroy the activity of the organs which prod uce it, or if we inject the blood of one animal into the veins of another, at all events, if we carry this be- yond certain limits, death is the consequence. If we could introduce into a tree woody fibre in a state of solution, it would be the same thing as placing a potato plant to vegetate in a paste of starch. The office of the leaves is to form starch, woody fibre, and sugar; consequently, if we convey these substances through the roots, the vital func- tions of the leaves must cease, and if the process of assimilation cannot take another form, the plant must die. Other substances must be present in a plant, besides the starch, sugar and gum, if these are to take part in the developement of the germ, leaves, and first radicle fibres. There is no doubt that a grain of wheat con- tains within itself the component parts of the germ and of the radicle fibres, and, we must suppose, exactly in the proportion ne- cessary for their formation. These compo- nent parts are starch and gluten; and it is evident that neither of them alone, but that both simultaneously assist in the formation of the root, for they both suffer changes under the action of air, moisture, and a suit- able temperature. The starch is converted into sugar, and the gluten also assumes a new form, and both acquire the capability of being dissolved in water, and of thus being conveyed to every part of the plant. Both the starch and the gum are completely con- sumed in the formation of the first part of the roots and leaves; and excess of either could not be used in the formation of leaves, or in any other way. The conversion of starch into sugar during the germination of grain is ascribed to a vegetable principle called diastase, which is generated during the act of commencing germination. But this mode of transforma- tion can also be effected by gluten, although it requires a longer time. Seeds, which have germinated, always contain much more dias- tase than is necessary for the conversion of their starch into sugar, for five parts by weight of starch can be converted into sugar by one part of malted barley. This excess of diastase can by no means be regarded as accidental, for, like the starch, it aids in the formation of the first organs of the young plant, and disappears with the sugar; diastase contains nitrogen and furnishes the elements of ve- getable albumen. Carbonic acid, water, and ammonia, are the food of fully-developed plants; starch, sugar, and gum, serve, when accompanied jy an azotised substance, to sustain the em- }ryo, until its first organs of nutrition are unfolded. The nutrition of a foetus and de- velopement of an egg proceed in a totally different manner from that of an animal which is separated from its parent ; the ex- ;lusion of air does not endanger the life of he fcetus, but would certainly cause the death of the independent animal. In the same manner, pure water is more advan- ageous to the growth of a young plant, :han that containing carbonic acid, but after a month the reverse is the case. The formation of sugar in maple-trees does not take place in the roots, but in the woody substance of the stem. The quantity of sugar in the sap augments, until it reaches THE ART OF CULTURE. 47 a certain height in the stem of the plant, above which point it remains stationary. Just as germinating barley produces a substance which, in contact with starch,, causes it to lose its insolubility and to be- come sugar, so in the roots of the maple, at the commencement of vegetation, a sub- stance must be formed, which, being dis- solved in water, permeates the wood of the trunk, and converts into sugar the starch, or whatever it may be, which it finds deposited there. It is certain, that when a hole is bored into the trunk of a maple-tree just above its roots, filled with sugar, and then closed again, the sugar is dissolved by the ascending sap. It is further possible that this sugar may be disposed of in the same manner as that formed in the trunks; at all events it is certain, that the introduction of it does not prevent the action of the juice npon the starch, and since the quantity of the sugar present is now greater than can be exhausted by the leaves and buds, it is excreted from the surface of the leaves or bark. Certain diseases of trees, for example that called honey-dew, evidently depend on the want of the due proportion between the quantity of the azotised and that of the un- azotised substances which are applied to them as nutriment. In whatever form, therefore, we supply plants with those substances which are the products of their own action, in no instance do they appear to have any effect upon their growth, or to replace what they have lost. Sugar, gum, and starch, are not food for plants, and the same must be said of humic acid, which is so closely allied to them in composition. If now we direct our attention to the par- ticular organs of a plant, we find every fibre and every particle of wood surrounded by a juice containing an azotised matter; while the starch, granules, and sugar are enclosed in cells formed of a substance containing ni- trogen. Indeed every where, in all the juices of the fruits and blossoms, we find a sub- stance destitute of nitrogen, accompanied by one which contains that element. The wood of the stem cannot be formed, quasi wood, in the leaves, but another sub- stance must be produced which is capable of being transformed into wood. This sub- stance must be in a state of solution, and accompanied by a compound containing ni- trogen; it is very probable that the wood and the vegetable gluten, the starch granules and the cells containing them, are formed simultaneously, and in this case a certain fixed proportion between them would be a condition necessary for their production. According to this view, the assimilation of the substances generated in the leaves will (cczteris paribus) depend on the quan- tity of nitrogen contained in the food. When a sufficient quantity of nitrogen is not pre- sent to aid in the assimilation of the sub- stances which do not contain it, these sub- stances will be separated as excrements from the bark, roots, leaves, and branches. The exudations of mannite, gum, and sugar, in strong and healthy plants cannot be ascribed to any other cause.* Analogous phenomena are presented by the process of digestion in the human or- ganism. In order that the loss which every part of the body sustains by the processes of respiration and perspiration may be re- stored to it, the organs of digestion require to be supplied with food, consisting of sub- stances containing nitrogen, and of others destitute of it, in definite proportions. If the substances which do not contain nitrogen preponderate, either they will be expended in the formation of fat, or they will pass unchanged through the organism. This is particularly observed in those people who live almost exclusively upon potatoes ; their excrements contain a large quantity of un- changed granules of starch, of which no trace can be detected when gluten or flesh is taken in proper proportions, because in this case the starch has been rendered capa- ble of assimilation. Potatoes, which when mixed with hay alone are scarcely capable of supporting the strength of a horse, form with bread and oats a strong and wholesome fodder. It will be evident from the preceding con- siderations, that the products generated by a plant may vary exceedingly, according to the substances given it as food. A super- abundance of carbon in the state of carbonic acid conveyed through the roots of plants, without being accompanied by nitrogen, cannot be converted either into gluten, ar- bumen, wood, or any other component part of an organ ; but either it will be separated in the form of excrements, such as sugar, starch, oil, wax, resin, mannite, or gum, or these substances will be deposited in greater or less quantity in the wide cells and vessels. The quantity of gluten, vegetable albu- men, and mucilage, will augment when plants are supplied with an excess of food containing nitrogen; and ammoniacal salts will remain in the sap, when, for example, in the culture of the beet, we manure the soil with a highly nitrogenous substance, or when we suppress the functions of the leaves by removing them from the plant. We know that the ananas is scarcely eatable in its wild state, and that it shoots forth a great quantity of leaves when treated with rich animal manure, without the fruit on that account acquiring a large amount of sugar; that the quantity of starch in po- tatoes increases when the soil contains much humus, but decreases when the soil is ma- * M. Trapp, in Giessen, possesses a Cleroden- dronfragrans, which grows in the house, and ex- udes on the surface of its leaves in September large colourless drops of sugar-candy, which form regular crystals upon drying ; I am not aware whether the juice of this plant contains sugar. Professor Redtenbacher, of Prague, informs me that he has analysed the crystals, and found them to be perfectly pure sugar. ED. 48 AGRICULTURAL CHEMISTRY. nured with strong animal manure, although then the number of cells increases, the po- tatoes acquiring in the first case a mealy, in the second a soapy, consistence. Beet-roots, taken from a barren, sandy soil, contain a maximum of sugar, and no arnmoniacal salts; and 4he Teltowa parsnep loses its mealy state in a manured land, because there all the circumstances necessary for the for- mation of cells are united.* An abnormal production of certain com- ponent parts of plants presupposes a power and capability of assimilation to which the most powerful chemical action cannot be compared. The best idea of it may be formed by considering that it surpasses in power the strongest galvanic battery, with which we are not able to separate the oxy- gen from carbonic acid. The affinity of chlorine for hydrogen, and its power to de- compose water under the influence of light and set at liberty its oxygen, cannot be con- sidered as at all equalling the power and energy with which a leaf separated from a plant decomposes the carbonic acid which it absorbs. The common opinion, that only the direct solar rays can effect the decomposition of carbonic acid in the leaves of plants, and that reflected or diffused light does not pos- sess this property, is wholly an error, for exactly the same constituents are generated in a number of plants, whether the direct rays of the sun fall upon them, or whether they grow in the shade. They require light, and indeed sun-light, but it is not necessary that the direct rays of the sun reach them. Their functions certainly proceed with greater intensity and rapidity in sunshine than in the diffused light of day; but there is nothing more in this than the similar action which light exercises on ordinary chemical combinations ; it merely accelerates in a greater or less degree the action already subsisting. Thus chlorine and hydrogen combining form muriatic acid. This combination is effected in a few hours in common daylight, but it ensues instantly, with a violent ex- plosion, under exposure to the direct solar rays, whilst not the slightest change in the two gases takes place in perfect darkness. When the liquid hydrocarburet of chlorine, resulting from the union of the olefiant gas of the associated Dutch chemists with chlo- rine, is exposed in a vessel with chlorine gas to the direct solar rays, chloride of car- bon is immediately produced ; but the same compound can be obtained with equal faci- lity in the diffused light of day, a longer time only being required. When this experiment is performed in the way first mentioned, two * Children fed upon arrow-root, salep, or in- deed any kind of amylaceous food, which does not contain ingredients fitted for the formation of bones and muscles, become fat, and acquire much embonpoint ; their limbs appear full, but they do not acquire strength, nor are their organs pro- perly developed. I products only are observed (muria'ic acid and perchloride of carbon); whilst by the latter method a class of intermediate bodies are produced, in which the quantity of chlo- rine constantly augments, until at last the whole liquid hydrocarburet of chlorine is converted into the same two products as in the first case. Here, also, not the slightest trace of decomposition takes place in the dark. Nitric acid is decomposed in common daylight into oxygen, and peroxide of nitro- gen ; and chloride of silver becomes black in the diffused light of day, as well as in the direct solar rays ; in short, all actions of a similar kind proceed in the same way in dif- fused light as well as in the solar light, the only difference consisting in the time in which they are effected. It cannot be other- wise in plants, for the mode of their nutri- ment is the same in all, and their component substances afford proof that their food has suffered absolutely the same change, whether they grow in the sunshine or in the shade. All the carbonic acid, therefore, which we supply to a plant will undergo a trans- formation, provided its quantity be not greater than can be decomposed by the leaves. We know that an excess of car- bonic acid kills plants, but we know also that nitrogen to a certain degree is not essen- tial for the decomposition of carbonic acid. All the experiments hitherto instituted prove, that fresh leaves placed in water impregnated with carbonic acid, and exposed to the in- fluence of solar light, emit oxygen gas, whilst the carbonic acid disappears. Now in these experiments no nitrogen is supplied at the same time with the carbonic acid; hence no other conclusion can be drawn from them than that nitrogen is not neces- sary for the decomposition of carbonic acid, for the exercise, therefore, of one of the functions of plants. And yet the presence of a substance containing this element ap- pears to be indispensable for the assimilation of the products newly formed by the decom- position of the carbonic acid, and their con- sequent adaptation for entering into the composition of the different organs. The carbon abstracted from the carbonic acid acquires in the leaves a new form, in which it is soluble and transferable to all parts of the plant. In this new form the carbon aids in constituting several new pro- ducts ; these are named sugar when they possess a sweet taste, gum or mucilage when tasteless, and excrementitious matters when expelled by the roots. Hence it is evident that the quantity and quality of the substances generated by the vital processes of a plant will vary accord- ing to the proportion of the different kinds of food with which it is supplied. The de- velopement of every part of a plant in a free and uncultivated state depends on the amount and nature of the food afforded to it by the spot on which it grows. A plant is developed on the most sterile and unfruitful soil as well as on the most luxuriant and THE ART OF CULTURE. 49 fertile, the only difference which can be ob- served being in its height and size, in the number of its twigs, branches, leaves, blos- soms, and fruit. Whilst the individual or- gans of a plant increase on a fertile soil, they diminish on another where those sub- stances which are necessary for their forma- tion are not so bountifully supplied ; and the proportion of the constituents which contain nitrogen and of those which do not in plants varies with the amount of nitro- genous matters in their food. The developement of the stem, leaves, blossoms, and fruit of plants is dependent on certain conditions, the knowledge of which enables us to exercise some influence on their internal constituents as well as on their size. It is the duty of the natural philoso- pher to discover what these conditions are j for the fundamental principles of agriculture must be based on a knowledge of them. There is no profession which can be com- pared in importance with that of agricul- ture, for to it belongs the production of food for man and animals ; on it depends the welfare and developement of the whole human species, the riches of states, and all commerce. There is no other profession in which the application of correct principles is productive of more beneficial effects, or is of greater and more decided influence. Hence it appears quite unaccountable, that we may vainly search for one leading prin- ciple in the writings of agriculturists and vegetable physiologists. The methods employed in the cultivation of land are different in every country, and in every district; and when we inquire the causes of these differences, we receive the answer, that they depend upon circum- stances. (Les circonstances font les assole merits.) No answer could show ignorance more plainly, since no one has ever yet de- voted himself to ascertain what these cir- cumstances are. Thus also when we inquire in what manner manure acts, we are an- swered by the most intelligent men, that its action is covered by the veil of Isis ; and when we demand further what this means, we discover merely that the excrements of men and animals are supposed to contain an incomprehensible something which assists in the nutrition of plants, and increases their size. This opinion is embraced without even an attempt being made to discover the component parts of manure, or to become acquainted with its nature. In addition to the general conditions, such as heat, light, moisture, and the component parts of the atmosphere, which are neces- sary for the growth of all plants, certain substances are found to exercise a peculiar influence on the developement of particular families. These substances either are al- ready contained in the soil, or are supplied to it in the form of the matters known under the general name of manure. But what does the soil contain, and what ate the com- ponents of the substances used as manure? Until ihese points are satisfactorily deter- mined, a rational system of agriculture can- not exist. The power and knowledge of the physiologist, of the agriculturist and chemist, must be united for the complete solution of these questions ; and in order to attain this end, a commencement must be made. The general object of agriculture is to produce in the most advantageous manner certain qualities, or a maximum size, in certain parts or organs of particular plants. Now, this object can be attained only by the application of those substances which we know to be indispensable to the developement of these parts or organs, or by supplying the conditions necessary to the production of the qualities desired. The rules of a rational system of agricul- ture should enable us, therefore, to give to each plant that which it requires for the at- tainment of the object in view. The special object of agriculture is to ob- tain an abnormal developement and produc- tion of certain parts of plants, or of certain vegetable matters, which are employed as food for man and animals, or for the pur- pose of industry. The means employed for effecting these two purposes are very different. Thus the mode of culture,' employed for the purpose of procuring fine pliable straw for Floren- tine hats, is the very opposite to that which must be adopted in order to produce a maxi- mum of corn from the same plant. Peculiai methods must be used for the production ot nitrogen in the seeds, others for giving strength and solidity to the straw, and others again must be followed when we wish to give such strength and solidity to the straw as will enable it to bear the weight of the ears. We must proceed in the culture of plants in precisely the same manner as we do in the fattening of animals. The flesh of the stag and roe, or of wild animals in general, is quite devoid of fat, like the muscular flesh of the Arab ; or it contains only small quan- tities of it. The production of flesh and fat may be artificially increased ; all domestic animals, for example, contain much fat. We give food to animals, which increases the activity of certain organs, and is itself capable of being transformed into fat. We add to the quantity of food, or we lessen the processes of respiration and perspiration by preventing motion. The conditions neces- sary to effect this purpose in birds are dif- ferent from those in quadrupeds ; and it is well known that charcoal powder produces such an excessive growth of the liver of a goose, as at length causes the death of the animal. The increase or diminution of the vital activity of vegetables depends only on heat and solar light, which we have not arbitra- rily at our disposal : all that we can do is to supply those substances which are adapted for assimilation by the power already pre- sent in the organs of the plant. But what F. 50 AGRICULTURAL CHEMISTRY. then are these substances? They may easily be detected by the examination of a soil, which is always fertile in given cosmi- cal and atmospheric conditions ; for it is evident, that the knowledge of its state and composition must enable us to discover the circumstances under which a sterile soil may be rendered fertile. It is the duty of the chemist to explain the composition of a fertile soil, but the discovery of its proper state or condition belongs to the agricultu- rist ; our present business lies only with the former. Arable land is originally formed by the crumbling of rocks., and its properties de- pend on the nature of their principal com- ponent parts. Sand, clay, and lime, are the names given to the principal constituents of the different kinds of soil. Pure sand and pure limestones, in which there are no other inorganic substances ex- cept siliceous earth, carbonate or silicate of lime, form absolutely barren soils. But ar- gillaceous earths form always a part of fer- tile soils. Now from whence come the argillaceous earths in arable land, what are their constituents, and what part do they play in favouring vegetation ? They are produced by the disintegration of aluminous minerals by the action of the weather; the common potash and soda felspars, Labrador spar, mica, and the zeolites, are the most common aluminous earths, which undergo this change. These minerals are found mixed with other substances in granite, gneiss, mica-slate, porphyry, clay-slate, grauwacke, and the volcanic rocks, basalt, clinkstone, and lava. In the grauwacke, we have pure quartz, clay-slate, and lime ; in the sandstones, quartz and loam. The transition limestone and the dolomites con- tain an intermixture of clay, felspar, por- phyry, and clay-slate; and the mountain limestone is remarkable for the quantity of argillaceous earths which it contains. Jura limestone contains 3 20, that of the Wur- temberg Alps 45 50 per cent, of these earths. And in the muschelkalk and the calcaire Dossier they exist in greater or less quantity. It is known, that the aluminous minerals are the most widely diffused on the surface of the earth, and as we have already men- tioned, all fertile soils, or soils capable of culture, contain alumina as an invariable constituent. There must, therefore, be something in aluminous earth which ena- bles it to exercise an influence on the life of plants, and to assist in their developement. The property on which this depends is that of its invariably containing potash and soda. Alumina exercises only an indirect influ- ence on vegetation, by its power of attract- ing and retaining water and ammonia; it is itself very rarely found in the ashes of plants,* but silica is always present, having * Alumina is generally supposed to be a com- mon ingredient of the ashes of plants, and it is m most places entered the plants by means of alkalies. In order to form a distinct con- ception of the quantities of alkalies in alu- minous minerals, it must be remembered that felspar contains 17| per cent, of potash, albite 1 1 -43 per cent, of soda, and mica 3 5 per cent.; and that zeolite contains 13 16 per cent, of both alkalies taken together. The late analyses of Ch. Gmelin, Lowe, Fricke, Meyer, and Redtenbacher, have also shown, that basalt contains from | to 3 per cent, of potash, and from 5 7 per cent, of soda, that clay slate contains from 2-75 3'31 per cent, of potash, and loam froml ^ 4 per cent, of potash. If, now, we calculate from these data, and from the specific weights of the different substances, how much potash must be con- tained in a layer of soil, which has been formed by the disintegration of 26,910 square feet (1 Hessian acre^ of one of these rocks to the depth of 20 inches, we find that a soil of Felspar contains 1,675,000 Ibs. Clink-stone " from 220,000 to 440,000 " Basalt " " 52,300 " 82,600 " Clay-slate " " 110,000 " 220,000 " Loam, " " 95,000 " 330,000 " Potash is present in all clays ; according to Fuchs, it is contained even in marl ; it has been found in all the argillaceous earths in which it has been sought. The fact that they contain potash may be proved in the clays of the transition and stratified moun- tains, as well as in the recent formations surrounding Berlin, by simply digesting them with sulphuric acid, by which process alum is formed. (Mitscherlich.) It is well known also to all manufacturers of alum, that the leys contain a certain quantity of this salt ready formed, the potash of which has its origin from the ashes of the stone and brown coal, which contain much argil- laceous earth. When we consider this extraordinary dis- tribution of potash over the surface of the earth, is it reasonable to have recourse to the idea, that the presence of this alkali in plants is due to the generation of a metallic oxide by a peculiar organic process from the component parts of the atmosphere ? This opinion found adherents even after the method of detecting potash in soils was known, and suppositions of the same kind may be found even in the writings of some physiologists of the present day. Such opinions belong properly to the time when flint was conceived to be a product of chalk, and when every thing which appeared in- comprehensible on account of not having been investigated, was explained by assump- tions far more incomprehensible. very frequently stated in the results of their analyses ; but in most cases it has been mistaken for phosphate of magnesia, or phosphate of alu- mina, with which it has many properties in com- mon, and from which it cannot be distinguished without much care and attention. ED. THE ART OF CULTURE. 51 A thousandth part of loam mixed with the quartz in new red sandstone, or with the lime in the different limestone forma- tions, affords as much potash to a soil only twenty inches in depth as is sufficient to supply a forest of pines growing upon it for a century. A single cubic foot of felspar is sufficient to supply a wood, covering a surface of 26,910 square feet, with the potash required for five years. Land of the greatest fertility contains argillaceous earths and other disintegrated minerals with chalk and sand in such a pro- portion as to give free access to air and moisture. The land in the vicinity of Vesu- vius may be considered as the type of a fer- tile soil, and its fertility is greater or less in different parts, according to the proportion of clay or sand which it contains. The soil which is formed by the disinte- gration of lava, cannot possibly, on account of its origin, contain the smallest trace of vegetable matter, and yet it is well known that when the volcanic ashes have been ex- posed for some time to the influence of air and moisture, a soil is gradually formed in which all kinds of plants grow with the greatest luxuriance. This fertility is owing to the alkalies which are contained in the lava, and which by exposure to the weather axe rendered capable of being absorbed by plants. Thousands of years have been ne- cessary to convert stones and rocks into the soil of arable land, and thousands of years more will be requisite for their perfect re- duction, that is, for the complete exhaustion of their alkalies. We see from the composition of the water in rivers, streamlets, and springs, how little rain-water is able to extract alkali from a soil, even after a term of years ; this water is generally soft, and the common salt, which even the softest invariably contains, proves that those alkaline salts, which are carried to the sea by rivers and streams, are returned again to the land by wind and rain. Nature itself shows us what plants re- quire at the commencement of the develope- ment of their germs and first radicle fibres. Bequerel has shown that the gramince, leguminosa, cruciferce, cichoracea, umbelli- fercR, coniferce, and cucurbitacecB emit acetic acid during germination. A plant which has just broken through the soil, and a leaf just burst open from the bud, furnish ashes by incineration, which contain as much, and generally more, of alkaline salts than at any period of their life. (De Saussure.) Now we know also, from the experiments of Bequerel, in what manner these alkaline salts enter young plants; the acetic acid formed during germination is diffused through the wet or moist soil, becomes saturated with lime, magnesia, and alkalies, and is again absorbed by the radicle fibres in the form of neutral salts. After the ces- sation of life, when plants are subjected to decomposition by means of decay and putre- faction, the soil receives again that whicb had been extracted from it. Let us suppose that a soil has been formed by the action of the weather on the compo- nent parts of granite, grauwacke, mountain limestone, or porphyry* and that nothing has vegetated on it for thousands of years. Now this soil would become a magazine of alkalies in a condition favourable for their assimilation by the roots of plants. The interesting experiments of Struve have proved that water impregnated with carbonic acid decomposes rocks which con- tain alkalies, and then dissolves a part of the alkaline carbonates. It is evident that plants also, by producing carbonic acid during their decay, and by means of the acids which exude from their roots in the living state, contribute no less powerfully to destroy the coherence of rocks. Next to the action of air, water, and change of tempera- ture, plants themselves are the most power- ful agents in effecting the disintegration of rocks. Air, water, and the change of temperature prepare the different species of rocks for yielding to plants the alkalies which they contain. A soil which has been exposed for centuries to all the influences which affect the disintegration of rocks, but from which the alkalies have not been removed, will be able to afford the means of nourish- ment to those vegetables which require alkalies for its growth during many years but it must gradually become exhausted, unless those alkalies which have been re- moved are again replaced ; a period, there- fore, will arrive when it will be necessary to expose it from time to time to a farther disintegration, in order to obtain a new sup- ply of soluble alkalies. For small as is the quantity of alkali which plants require, it is nevertheless quite indispensable for their perfect developement. But when one or more years have elapsed without any alka- lies having been extracted from the soil, a new harvest may be expected. The first colonists of Virginia found a country the soil of which was similar to that mentioned above; harvests of wheat and tobacco were obtained for a century from one and the same field, without the aid of manure ; but now whole districts are con- verted into unfruitful pasture-land, which without manure produces neither wheat nor tobacco. From every acre of this land there were removed in the space of one hundred years 12,000 Ibs. of alkalies in leaves, grain, and straw ; it became unfruitful, therefore, because it was deprived of every particle of alkali, which had been reduced to a soluble state, and because that which was rendered soluble again in the space of one year was not sufficient to satisfy the demands of the plants. Almost all the cultivated land in Europe is in this condition ; fallow is th* term applied to land left at rest for farther disintegration. It is the greatest possible mistake to suppose that the temporary dim> 52 AGRICULTURAL CHEMISTRY. nution of fertility in a soil is owing to the loss of humus ; it is the mere consequence of the exhaustion of the alkalies. Let us consider the condition of the coun- try around Naples, which is famed for its fruitful corn-land; the farms and villages are situated from eighteen to twenty-four miles distant from one another, and between them there are no roads, and consequently no transportation of manure. Now corn has been cultivated on this land for thousands of years, without any part of that which is annually removed from the soil being artifi- cially restored to it. How can any influ- ence be ascribed to humus under such cir- cumstances, when it is not even known whether humus was ever contained in the soil? The method of culture in that district completely explains the permanent fertility. It appears very bad in the eyes of our agri- culturists, but there it is the best plan which could be adopted. A field is cultivated once every three years, and is in the intervals allowed to serve as a sparing pasture for cattle. The soil experiences no change in the two years during which it there lies fal- low, farther than that it is exposed to the influence of the weather, by which a fresh portion of the alkalies contained in it are again set free or rendered soluble. The ani- mals fed on these fields yield nothing to these soils which they did not formerly pos- sess. The weeds upon which they live spring from the soil, and that which they return to it as excrement must always be less than that which they extract. The fields, therefore, can have gained nothing from the mere feeding of cattle upon them ; on the contrary, the soil must have lost some of its constituents. Experience has shown in agriculture that wheat should not be cultivated after wheat on the same soil, for it belongs with tobacco to the plants which exhaust a soil. But if the humus of a soil gives it the power of producing corn, how happens it that wheat does not thrive in many parts of Brazil, where the soils are particularly rich in this substance, or in our own climate, in soils formed of mouldered wood; that its stalk under these circumstances attains no strength, and droops prematurely? The cause is this, that the strength of the stalk is due to silicate of potash, and that the corn requires phosphate of magnesia, neither of w hie h substances a soil of humus can afford, since it does not contain them; the plant may, indeed, under such circumstances, be- come an herb, but will not bear fruit. Again, how does it happen that wheat does not flourish on a sandy soil, and that a calcareous soil is also unsuitable for its growth, unless it be mixed with a consider- able quantity of clay 1* It is because these * In consequence of these remarks in the former edition of this work, Professor Wohler of Gottin- gen has made several accurate analyses of diffe- soils do not contain alkalies in sumciert quantity, the growth of wheat being arrested by this circumstance, even should all othei substances be presented in abundance. It is not mere accident that only trees ot the fir tribe grow on the sandstone and lime- stone of the Carpathian mountains and the Jura, whilst we find on soils of gneiss, mica- slate, and granite in Bavaria, of clinkstone on the Rhone, of basalt in Vogelsberge, and of clay-slate on the Rhine and Eifel, the finest forests of other trees, which cannot be produced on the sandy or calcareous soils upon which pines thrive. It is explained the fact that trees, the leaves of which are renewed annually, require for their [eaves six to ten times more alkalies than the fir-tree or pine, and hence when they are placed in soils in which alkalies are con- tained in very small quantity, do not attain maturity.* When we see such trees grow- ing on a sandy or calcareous soil the red- beech, the service-tree, and the wild-cherry for example, thriving luxuriantly on lime- stone, we may be assured that alkalies are present in the soil, for they are necessary to their existence. Can we, then, regard it as remarkable that such trees should thrive in America, on those spots on which forests of pines which have grown and collected alkalies for centuries, have been burnt, and to which the alkalies are thus at once re- stored ; or that the Spartium scoparium, Erysimum latifolium, Blitwn capitatum, Se- necio viscosus, plants remarkable for the quantity of alkalies contained in their ashes, should grow with the greatest luxuriance on the localities of conflagrations ?f Wheat will not grow on a soil which has produced wormwood, and vice versa, worm- wood does not thrive where wheat has frown, because they are mutually preju- icial by appropriating the alkalies of the soil. One hundred parts of the stalks of wheat yield 15-5 parts of ashes (H. Davy;) the same quantity of the dry stalks of barley, rent kinds of limestone belonging to the secondary and tertiary formations. He obtained the remark- able result, that all those limestones, by the dis- integration of which soils adapted for the culture of wheat are formed, invariably contain a certain quantity of potash. The same observation haa also recently been made by M. Kuhlman of Lille. The latter observed that the efflorescence on the mortar of walls consists of the carbonates of soda and potash. * One thousand parts of the dry leaves of oaks yielded 55 parts of ashes, of which 24 parts con- sisted of alkalies soluble in water; the same quantity of pine-leaves gave only 29 parts of ashes, which contain 4.6 parts of soluble salts. (De Saussure.) t After the great fire in London, large quanti- ties of the Erysimum latifolium where observed growing on the spots where a fire had taken place. On a similar occasion the Blitum capitatum waa seen at Copenhagen, the Senecio viseosus in Nas- sau, and the Spartium scoparium in Languedoc. ; After the burnings of forests of pines in North | America, poplars grew on the same soil. THE ART OP CULTURE. 8-54 parts (Schrader;) and one hundred parts of the stalks of oats, only 4*42; the ashes of all these are of the same compo- sition. We have in these facts a clear proof of what plants require for their growth. Upon the same field, which will yield only one harvest of wheat, two crops of barley and three of oats may be raised. All plants of the grass kind require sili- cate of potash. Now this is conveyed to the soil, or rendered soluble in it by the irri- gation of meadows. The equiselacece, the reeds and species of cane, for example, which contain such large quantities of sili- ceous earth, or silicate of potash, thrive luxuriantly in marshes, in argillaceous soils, and in ditches, streamlets, and other places where the change of water renews con- stantly the supply of dissolved silica. The amount of silicate of potash removed from a meadow in the form of hay is very con- siderable. Wemeed only call to mind the melted vitreous mass found on a meadow between Manheim and Heidelberg after a thunder-storm. This mass was at first sup- posed to be a meteor, but was found on ex- amination (by Gmelin) to consist of silicate of potash ; a flash of lightning had struck a stack of hay, and nothing was found in its place except the melted ashes of the hay. Potash is not the only substance necessary for the existence of most plants; indeed it has been already shown that the potash may be replaced in many cases by soda, magne- sia, or lime; but other substances besides alkalies are required to sustain the life of plants. Phosphoric acid has been found in the ashes of all plants hitherto examined, and always in combination with alkalies or alka- line earths.* Most seeds contain certain quantities of phosphates. In the seeds of different kinds of corn particularly, there is abundance of phosphate of magnesia. Plants obtain their phosphoric acid from the soil. It is a constituent of all land capa- ble of cultivation, and even the heath at Luneburg contains it in appreciable quan- tity. Phosphoric acid has been detected * Professor Connall was lately kind enough to show me about half an ounce of a saline powder, which had been taken from an interstice in the body of a piece of teak timber. It consisted es- sentially of phosphate of lime, with small quan- tities of carbonate of lime and phosphate of mag- nesia. This powder had been sent to Sir David Brewster from India, with the assurance that it was the same substance which usually is found in the hollows of teak timber. It has long been known that silica, in the form of tabasheer, is se- creted by the bamboo ; but I am not aware that phosphates have been found in the same condi- tion. Without more precise information, we must therefore suppose that they are left in the hollows by the decay of the wood. Decay is a slow pro- cess of combustion, and the incombustible ashes must remain after the organic matter has been consumed. But if this explanation be correct, the wood of the teak-tree must contain an enormous quantity of earthy phosphates. ED. also in all mineral waters in which its pre- sence has been tested ; and in those in which it has not been found it has not been sought for. The most superficial strata of the deposits of sulphuret of lead (galena) contain crystallised phosphate of lead (green- lead ore ;) clay-slate, which forms extensive strata, is covered in many places with crys- tals of phosphate of alumina (Wavellite ;) all its fractured surfaces are overlaid with it. Phosphate of lime (Jlpatite) is found even in the volcanic boulders on the Laacher See in the Eifel, near Andernach.* The soil in which plants grow furnishes them with phosphoric acid, and they in turn yield it to animals, to be used in the forma- tion of their bones, and of those constituents of the brain which contain phosphorus. Much more phosphorus is thus afforded to the body than it requires, when flesh, bread, fruit, and husks of grain are used for food, and this excess is eliminated in the urine and the solid excrements. We may form an idea of the quantity of phosphate of magnesia contained in grain, when we con- sider that the concretions in the caecum of horses consist of phosphate of magnesia and ammonia, which must have been ob- tained from the hay and oats consumed as food. Twenty-nine of these stones were taken after death from the rectum of a horse belonging to a miller, in Eberstadt, the total weight of which amounted to 3 Ibs. ; and Dr. F. Simon has lately described a similar concretion found in the horse of a carrier, which weighed 1^ Ib. It is evident that the seeds of corn could not be formed without phosphate of magne- sia, which is one of their invariable con- stituents; the plant could not under such circumstances reach maturity. Some plants, however, extract other mat- ters from the soil besides silica, potash, and phosphoric acid, which are essential con- stituents of the plants ordinarily cultivated.! These other matters, we must suppose, supply, in part at least, the place and per- form the functions of the substances just named. We may thus regard common salt, sulphate of potash, nitre, chloride of potas- sium, and other matters, as necessary con- stituents of several plants. Clay-slate contains generally small quan- tities of oxide of copper; and soils formed from micaceous schist contain some metallic fluorides. Now, small quantities of these substances also are absorbed into plants, al- though we cannot affirm that they are ne- cessary to them. It appears that in certain cases flouride of calcium may take the place of phosphate of lime in the bones and teeth; at least it is impossible otherwise to explain its constant presence in the bones of antediluvian ani- mals, by which they are distinguished from * See the analyses of soils in the Appendix, t For more minute information regarding soili see the supplementary chapter at the end of Part 1. AGRICULTURAL CHEMISTRY. those of a later period. The bones of hu- man skulls found at Pompeii contain as much fluoric acid as those of animals of a former world, for if they be placed in a state of powder in glass vessels, and digested with sulphuric acid, the interior of the ves- sel will, after twenty-four hours, be found powerfully corroded (Liebig;) whilst the bones and teeth of animals of the present day contain only traces of it. (Berzelius.) De Saussure remarked that plants require quantities of the component parts of soils in different stages of their developement; an observation of much importance in consider- ing the growth of plants. Thus wheat yielded 79-1000 of ashes a month before blos- soming, 54-1000 while in blossom, and 33-1000 after the ripening of the seeds. It is therefore evident that wheat, from the time of its flowering, restores a part of its organic constituents to the soil, although the phosphate of magnesia remains in the seeds. The fallow-time, as we have already shown, is that period of culture during which land is exposed to a progressive dis- integration by means of the influence of the atmosphere, for the purpose of rendering a certain quantity of alkalies capable of being appropriated by plants. Now, it is evident, that the careful tilling of fallow-land must increase and accelerate this disintegration. For the purpose of agri- culture, it is quite indifferent, whether the land is covered with weeds, or with a plant which does not abstract the potash inclosed in it. Now many plants in the family of the leguminosce are remarkable on account of the small quantity of alkalies or salts in general which they contain ; the Windsor bean (FiciaFaba,) for example, contains no free alkalies, and not one per cent, of the phosphates of lime and magnesia. (Einhof.) The bean of the kidney-bean (Phaseolus vulgaris) contains only traces of salts. (Bra- connot.) The stem of lucerne (Medicago xativa) contains only 0.83 per cent., that of the lentil (Ervum Lens} only 0.57 of phos- phate of lime with albumen. (Crome.) Buck-wheat dried in the sun yields only 0.681 per cent, of ashes, of which 0.09 parts are soluble salts. CZenneck.)* These plants * The small quantity of phosphates which the seeds of the lentils, beans, and peas contain, must be the cause of their small value as articles of nour- ishment, since they surpass all other vegetable food in the quantity of nitrogen which enters into their composition. But as tne component parts of the bones (phosphate of lime and magnesia) are absent, they satisfy the appetite without increasing the strength. The following is an analysis of lentils (Playfair.) 6.092 grammes lost 0.972 grammes of water at 212. 0.566 grammes, burned with ox- ide of copper, gave 0.910 grammes carbonic acid and 0.336 grammes of water. The lentils on combustion with oxide of copper, yielded a gas, in which the proportion of the nitrogen to the car bonic acid was as 1 to 16. Carbon 44.45 Hydrogen 6.59 Nitrogen 6.42 Water 15.95 Delong to those which are termed fallow- rops, and the cause wherefore they do noC exercise any injurious influence on corn which is cultivated immediately after them is, that they do not extract the alkalies of the soil, and only a very small quantity of phosphates. It is evident that two plants growing be- side each other will mutually injure one another, if they withdraw the same food from the soil. Hence it is not surprising that the wild chamomile (Matncaria Chamo- milld) and Scotch-broom (Spartium Scopa- riuni) impede the growth ol corn, when it is considered that both yield from 7 to 7.43 per cent, of ashes, which contain ^ of car* bonate of potash. The darnel and the flea- bane (Erigeron acre) blossom and bear fruit at the same time as corn, so that when growing mingled with it, they will partake of the component parts of the soil, and in proportion to the vigour of their growth, lhat of the corn must decrease; for what one receives, the others are deprived of. Plants will, on the contrary, thrive beside each other, either when the substances necessary for their growth which they ex- tract from the soil are of different kinds, or when they themselves are not both in the same stages of developement at the same time. On a soil, for example, which contains potash, both wheat and tobacco may be reared in succession, because the latter plant does not require phosphates, salts which are invariably present in wheat, but requires only alkalies, and food containing nitrogen. According to the analysis of Posselt and Riemann, 10,000 parts of the leaves of the tobacco-plant contain 16 parts of phosphate of lime, 8.8 parts of silica, and no magnesia; whilst an equal quantity of wheat straw contains 47.3 parts, and the same quantity of the grain of wheat 99.45 parts of phos- phates. (De Saussure.) Now, if we suppose that the grain of wheat is equal to half the weight of its straw, then the quantity of phosphates ex- tracted from a soil by the same weights of wheat and tobacco must be as 97.7 : 16. This difference is very considerable. The roots of tobacco, as well as those of wheat, extract the phosphates contained in the soil, but they restore them again, because they are not essentially necessary to the deve- lopement of the plant. CHAPTER VIII. ON THE ALTERNATION OF CROPS. IT has long since been found by experience, that the growth of annual plants is rendered imperfect, and their crops of fruit or herbs less abundant, by cultivating them in suc- cessive years on the same soil, and that, in spite of the loss of time, a greater quantity of grain is obtained when a field is allowed ALTERNATION OF CROPS. 55 to lie uncultivated for a year. During this interval of rest, the soil, m a great measure, regains its original fertility. It has been further observed, that certain plants, such as peas, clover, and flax, thrive on the same soil only after a lapse of years ; whilst others, such as hemp, tobacco, helian- thus tuberosus, rye, and oats may be culti- vated in close succession when proper ma- nure is used. It has also been found, that se- veral of these plants improve the soil, whilst others, and these are the most numerous, impoverish or exhaust it. Fallow turnips, cabbage, beet, spelt, summer and winter barley, rye and oats, are considered to be- long to the class which impoverish a soil ; whilst by wheat, hops, madder, late turnips, hemp, poppies, teasel, flax, weld, and lico- rice, it is supposed to be entirely exhausted. The excrements of man and animals have been employed from the earliest times for the purpose of increasing the fertility of soils ; and it is completely established by all experience, that they restore certain consti- tuents to the soil, which are removed with the roots, fruit or grain, or entire plants grown upon it. But it has been observed that the crops are not always abundant in proportion to the quantity of manure employed, even al- thou^h'it may have been of the most power- ful kind ; that the produce of many plants, for example, diminishes, in spite of the ap- parent replacement by manure of the sub- stances removed from the soil, when they are cultivated on the same field for several years in succession. On the other hand it has been remarked, that a field Avhich has become unfitted for a certain kind of plants was not on that ac- count unsuited for another; and upon this observation, a system of agriculture has been gradually founded, the principal ob- ject of which is to obtain the greatest possi- ble produce with the least expense of ma- nure. Now it was deduced from all the foregoing facts that plants require for their growth different constituents of soil, and it was very soon perceived, that an alternation of the plants cultivated maintained the fertility of a soil quite as well as leaving it at rest or plication of chemical discoveries ? A future generation, However, will derive incalcula- ble advantage from these means of help. Of all the views which have been adopted regarding the cause of the favourable effects of the alternations of crops, that proposed by M. Decandolle alone deserves to be men- tioned as resting on a firm basis. Decandolle supposes that the roots of plants imbibe soluble matter of every kind from the soil, and thus necessarily absorb a number of substances which are not adapted to the purposes of nutrition, and must sub- sequently be expelled by the roots, and re- turned to the soil as excrements. Now, as excrements cannot be assimilated by the plant which ejected them, the more of these matters which the soil contains, the more unfertile must it be for the plants of the same species. These excrementitious mat- ters may, however, still be capable of assi- milation by another kind of plants, which would thus remove them from the soil, and render it again fertile for the first. And if the plants last grown also expel substances from their roots, which can be appropriated as food by the former, they will improve the soil in two ways. Now a great number of facts appear at first sight to give a high degree of probabi- lity to this view. Every gardener knows that a fruit-tree cannot be made to grow on the same spot where another of the same species has stood ; at least not until after a lapse of several years. Before new vine- stocks are planted in a vineyard from which the old have been rooted out, other plants are cultivated on the soil for several years. In connexion with this it has been observed, that several plants thrive best when growing beside one another; and on the contrary, that others mutually prevent each other's developement. Whence it was concluded, that the beneficial influence in the former case depended on a mutual interchange of nutriment between the plants, and the in- jurious one in the latter on a poisonous action of the excrements of each on the other respectively. A series of experiments by Macaire- Princep gave great weight to this theory. He proved beyond all doubt that many fallow. It was evident that all plants must | plants are capable of emitting extractive give back to the soil in which they grow different proportions of certain substances, which are capable of being used as food by a succeeding generation. But agriculture has hitherto never sought aid from chemical principles, based on the knowledge of those substances which plants extract from the soil on which they grow, and of those restored to the soil by means of j manure. The discovery of such principles v/ill be the task of a future generation, for what can be expected from the present, which recoils with seeming distrust and aversion from all the means of assistance offered it by chemistry, and which does not understand the art of making a rational ap- matter from their roots. He found that the excretions were greater during the night than by day (?), and that the water in which plants of the family of the Legumi- nosce grew acquired a brown colour. Plants of the same species placed in water im- pregnated with these excrements were im- peded in their growth, and faded prema- turely, whilst, on the contrary, corn-plants grew vigorously in it, and the colour of the water diminished sensibly; so that it ap- peared as if a certain quantity of the excre- ments of the Leguminosce had really been absorbed by the corn-plants. These ex- periments afforded, as their main result, that the characters and properties oi the ex AGRICULTURAL CHEMISTRY. crements of different species of plants are different from one another, and that some plants expel excrementitious matter of an acrid and resinous character; others mild substances resembling gum. The former of these, according to Macaire- Princep , may be regarded as poisonous, the latter as nu-. tritious. The experiments of Macaire-Princep af- ford positive proof that the roots, probably of all plants, expel matters, which cannot be converted in their organism either into woody fibre, starch, vegetable albumen, or gluten, since their expulsion indicates that they are quite unfitted for this purpose. But they cannot be considered as a confir- mation of the theory of Decandolle, for they leave it quite undecided whether the sub- stances were extracted from the soil, or formed by the plant itself from food received from another source. It is certain that the gummy and resinous excrements observed by Macaire-Princep could not have been contained in the soil, and as we know that the carbon of a soil is not diminished by culture, but, on the contrary, increased, we must conclude that all excrements which contain carbon must be formed from the food obtained by plants from the atmosphere. Now, these excrements are compounds, produced in consequence of the transforma- tions of the food, and of the new forms which it assumes by entering into the com- position of the various organs. M. Decandolle's theory is properly a modification of an earlier hypothesis, which supposed that the roots of different plants extracted different nutritive substances from the soil, each plant selecting that which was exactly suited for its assimilation. Ac- cording to this hypothesis, the matters in- capable of assimilation are not extracted from the soil, whilst M. Decandolle consi- ders that they are returned to it in the form of excrements. Both views explain how it happens that after corn, corn cannot be raised with advantage, nor after peas, peas ; but they do not explain how a field is im- proved by lying fallow, and this in propor- tion to the care with which it is tilled and kept free from weeds; nor do they show how a soil gains carbonaceous matter by the cultivation of certain plants, such as lucerne and sainfoin. Theoretical considerations on the process of nutrition, as well as the experience of all agriculturists, so beautifully illustrated by the experiments of Macaire-Princep, leave no doubt that substances are excreted from the roots of plants, and that these matters form the means by which the carbon re- ceived from humus in the early period of their growth is restored to the soil. But we may now inquire whether these excre- ments, in the state in which they are ex- pelled, are capable of being employed as food by other plants. The excrements of a carnivorous animal contain no constituents fitted lor the nou- rishment of another of the same species; but it is possible that an herbivorous animal, a fish, or a fowl, might find in them undi- gested matters capable of being digested in their organism, from the very circumstance of their organs of digestion having a different structure. This is the only sense in which we can conceive that the excrements of one animal could yield matter adapted for the nutrition of another. A number of substances contained in the food of animals pass through their alimentary organs without change, and are expelled from the system ; these are excrements but not excretions. Now a part of such excre- mentitious matter might be assimilated in passing through the digestive apparatus of another animal. The organs of secretion form combinations of which only the ele- ments were contained in the food. The production of these new compounds is a consequence of the changes which the food undergoes in becoming chyle and chyme, and of the further transformations to which these are subjected by entering into the composition of the organism. These mat- ters, likewise, are eliminated in the excre- ments, which must therefore consist of two different kinds of substances, namely, of the indigestible constituents of the food, and of the new compounds formed by the vital pro- cess. The latter substances have been pro- duced in consequence of the formation of fat, muscular fibre, cerebral and nervous substance, and are quite incapable of being converted into the same substances in any other animal organism. Exactly similar conditions must subsist in the vital processes of plants. When sub- stances which are incapable of being em- ployed in the nutrition of a plant exist in the matter absorbed by its roots, they must be again returned to the soil. Such excre- ments might be serviceable and even indis- pensable to the existence of several other plants. But substances that are formed in a vegetable organism during the process of nutrition, which are produced, therefore, in consequence of the formation of woody fibre, starch, albumen, gum, acids, &c., cannot again serve in any other plants to form the same constituents of vegetables. The consideration of these facts enables us to distinguish the difference between the views of Decandolle and those of Macaire- Princep. The substances which the former physiologist viewed as excrements, belonged to the soil; they were undigested matters, which although not adapted for the nutrition of one plant might yet be indispensable to another. Those matters, on the contrary, designated as excrements by Macaire-Prin- cep, could only in one form serve for the nutrition of vegetables. It is scarcely ne- cessary to remark that this excrementitious matter must undergo a change before another season. During autumn and winter it be- gins to suffer a change from the influence of air and water; its putrefaction, and a* ALTERNATION OF CROPS. 57 length, by continued contact with the air, which tillage is the means of procuring, its decay are effected ; and at the commence- ment of spring it has become converted, either in whole or in part, into a substance which supplies the place of humus, by being a constant source of carbonic acid. The quickness with which this decay of the excrements of plants proceeds depends on the composition of the soil, and on its greater or less porosity. It will take place very quickly in a calcareous soil : for the power of organic excrements to attract oxy- gen and to putrefy is increased by contact with the alkaline constituents, and by the general porous nature of such kinds of soil, which freely permit the access of air. But it requires a longer time in heavy soils con- sisting of loam or clay. The same plants can be cultivated with advantage on one soil after the second year, but in others not until the fifth or ninth, merely on account of the change and de- struction of the excrements, which have an injurious influence on the plants being com- pleted in the one, in the second year; in the others, not until the ninth. Jn some neighbourhoods clover will not thrive till the sixth year, in otherjs not till the twelfth ; flax in the second or third year. All this depends on the chemical nature of the soil, for it has been found by experience that in those districts where the intervals at which the same plants can be cultivated with advantage are very long, the time can- not be shortened even by the use of the most powerful manures. The destruction of the peculiar excrements of one crop must have taken place before a new crop can be pro- duced. Flax, peas, clover, and even potatoes, are plants the excrements of which, in argilla- ceous soils, require the longest time for their conversion into humus; but it is evident that the use of alkalies and burnt lime, or even small quantities of ashes which have not been lixiviated, must enable a soil to permit the cultivation of the same plants in a much shorter time. A soil lying fallow owes its earlier fer- tility, in part, to the destruction or conver- sion into humus of the excrements contained in it, which is effected during the fallow season, at the same time that the land is exposed to a farther disintegration. In the soils in the neighbourhood of the Rhine and Nile, which contain much pot- ash, and where crops can be obtained in close succession from the same field, the fallowing of the land is superseded by the inundation ; the irrigation of meadows effects the same purpose. It is because the water of rivers and streams contains oxygen in solution that it effects the most complete and rapid putre- faction of the excrements contained in the soil which it penetrates, and in which it is continually renewed. If it was the water alone which produced this effect, marshy meadows should be most fertile. Hence it 8 is not sufficient in irrigating meadows to convert them into marshes, by covering for several months their surface with water, which is not renewed ; for the advantage of irrigation consists principally in supplying oxygen to the roots of plants. The quantity of water necessary for this purpose is very small, so that it is sufficient to cover the meadow with a very thin layer, if this be frequently renewed. The cultivation of meadows forms one of the most important branches of rural eco- nomy. It contributes materially to the pros- perity of the agriculturist by increasing his stock of cattle, and consequently by furnish- ing him with manure, which may be applied to the augmentation of his crops. Indeed, the great progress which has been made in Germany in the improvement of cattle is mainly attributable to the attention which is devoted in that country to the culture of meadows. The environs of Siegin, in Nas- sau, are particularly famed in this respect, and every year a large number of young farmers repair to it, for the purpose of study- ing this branch of agriculture in situ. In that district the culture of grass has attained such great perfection, that the produce of their meadow-land far exceeds that obtained in any other part of Germany. This is ef- fected simply by preparing the ground in such a manner as to enable it to be irrigated both in spring and in autumn. The surface of the soil is fitted to suit the locality, and the quantity of water which can be com- manded. Thus if the meadows be situated upon a declivity, banks of from one to two feet in height are raised at short distances from each other. The water is admitted by small channels upon the most elevated bank, and allowed to discharge itself over the sides in such a manner as to run upon the bank situated below. The grass grown upon meadows irrigated in this way is three or four times higher than that obtained from fields which are covered with water that is deprived of all egress and renewal. It follows from what has preceded that the advantage of the alternation of crops is ow- ing to two causes. A fertile soil ought to afford to a plant all the inorganic bodies indispensable for its ex- istence in sufficient quantity and in such condition as allows their absorption. All plants require alkalies, which are contained in some, in the Gh-aminece for ex- ample, in the form of silicates ; in otners, in mat of tartrates, citrates, acetates, or ox- alates. When these alkalies are in combination with silicic acid, the ashes obtained by the incineration of the plant contain no carbonic acid ; but when they are united with organic acids, the addition of a mineral acid to their ashes causes an effervescence. A third species of plants requires phos- phate of lime, another phosphate of mag- nesia, and several do not thrive without car- bonate of lime. 58? AGRICULTURAL CHEMISTRY. Silicic acid is the first solid substance taken up by plants ; it appears to be the ma- terial from which the formation of the wood takes its origin, acting like a grain of sand around which the first crystals form in a so- lution of a salt which is in the act of crys- tallising. Silicic acid appears to perform the functions of woody fibre in the Equise- tacece and bamboos,* just as the crystalline salt, oxalale of lime, does in many of the lichens. When we grow in the same soil for seve- ral years in succession different plants, the first of which leaves behind that which the second, and the second that which the third may require, the soil will be a fruitful one for all the three kinds of produce. If the first plant, for example, be wheat, which consumes the greatest part of the silicate of potash in a soil, whilst the plants which succeed it are of such a kind as require only small quantities of potash, as is the case with Leguminosce, turnips, potatoes, &c., the wheat may be again sowed with advantage after the fourth year ; for during the interval of three years the soil will, by the action of the atmosphere, be rendered capable of again yielding silicate of potash in sufficient quantity for the young plants. The same precaution must be observed with regard to the other inorganic constitu- ents, when it is desired to grow different plants in succession on the same soil : for a successive growth of plants which extract the same component parts, must gradually render it incapable of producing them. Each of these plants during its growth re- turns to the soil a certain quantity of sub- stances containing carbon, which are gra- dually converted into humus, and are for the most part equivalent to as much carbon as the plants had formerly extracted from the soil in a state of carbonic acid. But al- though this is sufficient to bring many plants to maturity, it is not enough to furnish their different organs with the greatest possible supply of nourishment. Now the object of agriculture is to produce either articles of commerce, or food for man and animals ; but a maximum of produce in plants is al- ways in proportion to the quantity of nutri- ment supplied to them in the first stage of their developement. The nutriment of young plants consists of carbonic acid, contained in the soil in the form of humus, and of nitrogen in the form of ammonia, both of which must be sup- plied to the plants, if the desired purpose is to be accomplished. The formation of am- monia cannot be effected on cultivated land, but humus may be artificially produced ; and this must be considered as an important ob- ject in the alternation of crops, and as the second reason of its peculiar advantages. * Silica is found in the joints of bamboos, in the form of small round globules, which have received the name of Tabasheer, and are distinguished by their remarkable optical properties. The sowing of a field with fallow plants, such as clover, rye, buck-wheat, &c., and the incorporation of plants, when nearly at blossom, with the soil, affect this supply of humus in so far, that young plants subse- quently growing in it find, at a certain pe- riod of their growth, a maximum of nu- triment, that is, matter in the process of de- cay. The same end is obtained, but with much greater certainty, when the field is planted with sainfoin or lucerne.* These plants are remarkable on account of the great ramifi- cation of their roots, and strong develope- ment of their leaves, and for requiring only a small quantity of inorganic matter. Until they reach a certain period of their growth, they retain all the carbonic acid and ammo- nia which may have been conveyed to them by rain and the air, for that which is not absorbed by the soil is appropriated by the leaves ; they also possess an extensive four or six-fold surface, capable of assimilating these bodies, and of preventing the volatili- zation of the ammonia from the soil, by completely covering it in. An immediate consequence of the pro- duction of the green principle of the leaves, and of their remaining component parts, as well as those of the stem, is the equally abundant excretion of organic matters into the soil from the roots. The favourable influence which this ex- ercises on the land, by furnishing it with matter capable of being converted into hu- mus, lasts for several years, but barren spots gradually appear after the lapse of some time. Now it is evident that, after from six to seven years, the ground must become so impregnated with excrements that every fibre of the root will be surrounded with them. As they remain for some time in a soluble condition, the plants must absorb part of them and suffer injurious effects in consequence, because they are not capable of assimilation. When such a field is ob- served for several years, it is seen that the barren spots are again covered with vegeta- tion, (the same plants being always sup- posed to be grown,) whilst new spots be- come bare and apparently unfruitful, and so on alternately. The causes which produce this alternate barrenness and fertility in the different parts of the land are evident. The excrements upon the barren spots receiving no new addition, and being subjected to the influence of air and moisture, they pass into putrefaction, and their injurious influence * The alternation of crops with sainfoin and lu- cern is now universally adopted in Bingen and its vicinity, as well as in the Palatinate ; the fields in these districts receive manure only once every nine years. In the first years after the land has been manured turnips are sown upon it, in the next following years barley, with sainfoin or lu- cerne ; in the seventh year potatoes, in the eighth wheat, in the ninth barley ; on the tenth year it is manured, and then the same rotation again takes place. ON MANURE. 59 ceases. The plants now find those sub- stances which formerly prevented their growth removed, and in their place meet with humus., that is, vegetable matter in the act of decay. We can scarcely suppose a better means of producing humus than by the growth of plants, the leaves of which are food for ani- mals ; for they prepare the soil for plants of every other kind, but particularly for those to which, as to rape and flax, the presence of humus is the most essential condition of growth. The reasons why this interchange of crops is so advantageous the principles which regulate this part of agriculture, are, there- fore, the artificial production of humus, and the cultivation of different kinds of plants upon the same field, in such an order of succession, that each shall extract only cer- tain components of the soil, whilst it leaves behind or restores those which a second or third species of plant may require for its growth and perfect developement. Now, although the quantity of humus in a soil may be increased to a certain degree by an artificial cultivation, still, in spite of this, there cannot be the smallest doubt that a soil must gradually lose those of its con- stituents which are removed in the seeds, roots, and leaves of the plants raised upon it. The fertility of a soil cannot remain un- impaired, unless we replace in it all those substances of which it has been thus de- prived. Now this is effected by manure. CHAPTER IX. OF MANURE. WHEN it is considered that every consti- tuent of the body of man and animals is de- rived from plants, and that not a single element is generated by the vital principle, it is evident that all the inorganic constitu- ents of the animal organism must be re- garded, in some respect or other, as manure. During their life, the inorganic components of plants which are not required by the ani- mal system, are disengaged from the orga- nism, in the form of excrements. After their death, their nitrogen and carbon pass into the atmosphere as ammonia and car- bonic acid, the products of their putrefac- tion, and at last nothing remains except the phosphate of lime and other salts in their bones. Now this earthy residue of the pu- trefaction of animals must be considered, in a rational system of agriculture, as a power- ful manure for plants, because that which has been abstracted from a soil for a series of years must be restored to it, if the land is to be kept in a permanent condition of fertility. ANIMAL MANURES. We may now inquire whether the excre- ments of animals, which are empkr, ctl as manure, are all of a like nature and power, and whether they, in every case, administer to the necessities of a plant by an identical mode of action. These points may easily j be determined by ascertaining the composi- tion of the animal excrements, because we shall thus learn what substances a soil really receives by their means. According to the common view, the action of solid animal excrements depends on the decaying orga- nic matters which replace the humus, and on the presence of certain compounds of nitrogen, which are supposed to be a.- lated by plants, and employed in the pro- duction of gluten and other azotised sub- stances. But this view requires further confirmation with respect to the solid excre- ments of animals, for they contain so small a proportion of nitrogen, that they cannot possibly by means of it exercise any in- fluence upon vegetation. We may form a tolerably correct idea of the chemical nature of the animal excre- ment without further examination, by com- paring the excrements of a dog with its food. When a dog is fed with flesh and bones, both of which consist in great part of organic substances containing nitrogen, a moist white excrement is produced, which crumbles gradually to a dry powder in the air. This excrement consists of the phos- phate of lime of the bones, and contains scarcely T ^Q part of its weight of foreign organic substances. The whole process ot nutrition in an animal consists in the pro- gressive extraction of all the nitrogen from the food, so that the quantity of this element found in the excrements must always be less than that contained in the nutriment. The analysis of the excrements of a horse by Macaire and Marcet proves this fact com- pletely. The portion of excrements sub- jected to analysis was collected whilst fresh, and dried MI vacuo over sulphuric acid ; 100 parts of it (corresponding to from 350 to 400 parts of the dung before being dried) contained 0.8 of nitrogen. Now every one who has had experience in this kind of ana- lysis is aware that a quantity under one per cent, cannot be determined with accuracy. We should, therefore, be estimating its pro- portion at a maximum, were we to consider it as equal to one-half per cent. It is cer- tain, however, that these excrements are not entirely free from nitrogen, for they emit ammonia when digested with caustic potash. The excrements of a cow, on combustion with oxide of copper, yielded a gas which contained one vol. of nitrogen gas, and 26.30 vol. of carbonic acid. 100 parts of fresh excrements contained Nitrogen . . . 0.506 Carbon .... 6.204 Hydrogen . . . 0.824 Oxygen .... 4.818 Ashes .... 1.748 Water . . . . 85.900 100.000 60 AGRICULTURAL CHEMISTRY. Now, according to the analysis of Bous- singault, which merits the greatest confi- dence, hay contains one per cent, of nitro- gen; consequently in the 25 Ibs. of hay which a cow consumes daily, of a Ib. of nitrogen must have been assimilated. This quantity of nitrogen entering into the com- position of muscular fibre would yield 8-3 Ibs. of flesh in its natural condition.* The daily increase in size of a cow is, however, much less than this quantity. We find that the nitrogen, apparently deficient, is actually contained in the milk and urine of the ani- mal. The urine of a milch-cow contains less nitrogen than that of one which does not yield milk ; and as long as a cow yields a plentiful supply of milk, it cannot be fat- tened. We must search for the nitrogen of the food assimilated, not in the solid, but in the liquid excrements. The influence which the former exercise on the growth of vege- tables does not depend upon the quantity of nitrogen which they contain. For if this were the case, hay should possess the same influence ; that is, from 20 to 25 Ibs. ought to have the same power as 100 Ibs. of fresh cow-dung. But this is quite opposed to all experience. Which then are the substances in the ex- crements of the cow and horse which exert an influence on vegetation 1 When horse-dung is treated with water, a portion of it to the amount of 3 or 3 per cent, is dissolved, and the water is coloured yellow. The solution is found to contain phosphate of magnesia, and salts of soda, besides small quantities of organic matters.f The portion of the dung undissolved by the water yields to alcohol a resinous substance possessing all the characters of gall which has undergone some change; while the residue possesses the properties of saw-dust, from which all soluble matter has been ex- tracted by water, and bums without any * 100 Ibs. of flesh contain on an average 15'86 of muscular fibre : 18 parts of nitrogen are con tained in 100 parts of the latter. t Dr. C. T. Jackson in his "Geological and Agricultural Survey of Rhode Island," (page 205.) gives the following analysis of horse-dung : 50C grains, dried at a heat a little above that of boiling water, lost 357 grains of water. The dry mass weighing 143 grains was burned, and left 8'5 grains of ashes, of which 4 - 80 grains were soluble in dilute nitric acid, and 3'20 insoluble. The ashes being analysed, gave Silica 3'2 Phosphate of lime . . . 0'4 Carbonate of lime . . .1*5 Phosphate of magnesia and soda . 2'9 8-0 It consists, then, of the following ingredients : Water .... 35VO Vegetable fibre and animal matter 135-0 Silica . . . . .3-2 Phosphate of lime . . 0'4 Carbonate of lime . . 1'5 Phosphate of magnesia and soda . 2 '9 500-0 ~mell. 100 parts of the fresh dung of a lorse being dried at 100 C. (212 F.) eave from 25 to 30 or 31 parts of solid sub- stances, and contained, accordingly, from 69 o 75 parts of water. From the dried ex- crements, we obtain, by incineration, vari- able quantities of salts and earthy matters, according to the nature of the food which las been taken by the animal. Macaire and Vlarcet found 27 per cent, in the dung ana- ysed by them ; I obtained only 10 per cent. Tom that of a horse fed with chopped straw, oats, and hay. It results then that with >om 3600 to 4000 Ibs. of fresh horse-dung, corresponding to 100 Ibs. of dry dung, we place on the land from 2484 to 3000 Ibs. of water, and from 730 to 900 Ibs. of vegetable matter and altered gall, and also from 100 to 270 Ibs. of salt and other inorganic sub- stances. The latter are evidently the substances to which our attention should be directed, for they are the same which formed the compo- nent parts of the hay, straw, and oats with which the horse was fed. Their principal constituents are the phosphates of lime and magnesia, carbonate of lime and silicate of potash; the first three of these preponde- rated in the corn, the latter in hay. Thus in 1000 Ibs. of horse-dung, we pre- sent to a field the inorganic substances con- tained in 6000 Ibs. of hay, or 8300 Ibs. of oats (oats containing 3'1 per cent, ashes ac- cording to De Saussure.) This is sufficient to supply l crop of wheat with potash and phosphates. The excrements of cows,* black cattle, and sheep, contain phosphate of lime, com- mon salt, and silicate of lime, the weight of which varies from 9 to 28 per cent., accord- ing to the fodder which the animal receives j the fresh excrements of the cow domain from 86 to 90 per cent, of water. Human faeces have been subjected to an exact analysis by Berzelius. When fresh they contain, beside of their weight 01 water, nitrogen in very variable quantity, namely, in the minimum l, in the maxi- * It has been formerly stated (page 41) that all the potash contained in the food of a cow is again, discharged in its excrements. The same also takes place with the other inorganic constituents of food, either when they are not adapted for as- similation, or when present in superabundant quantities. The value of manure may thus be artificially increased. We lately saw, for ex- ample, some cow-dung, sent by a farmer, who wished to ascertain the cause of its increased value. He had formerly employed this manure for his land, but with so little advantage that he found it more profitable to dry it, and use it as fuel. On inquiry, it was found, that his cows had been fed upon oil-cake. This species of food is particularly rich in phosphates. More of these salts being present than were requisite for the purpose of assimilation, they were removed from the system in the form of excrementitious matter, and in a condition adapted for the uses of plants. The fact that particular kinds of food enrich or impoverish the manure obtained from the cattle fed upon them, has repeatedly been observed. EE OP MANURE. Gl mum 5 per cent. In all cases, however, they were richer in this element than the excrements of other animals. Berzelius obtained by the incineration of 100 parts of dried excrements, 15 parts of ashes, which were principally composed of the phosphates of lime and magnesia. The following quantitative organic ana- lysis has recently been executed for the pur- pose of ascertaining the proportion of carbon, nitrogen, and inorganic matter contained in faeces, in comparison with the food taken.* (Playfair.) Carbon . . . 45'24 Hydrogen . . 6 '88 Nitrogen (average) . 4'00 Oxygen . . . 30'30 Ashes . . . 13-58 The inorganic matter contained in the excrements analyzed is nearly two per cent, less than that found by Berzelius ; but the proportion always vanes, according to the nature of the food. It is quite certain that the vegetable con- stituents of the excrements with which we manure our fields cannot be entirely without influence upon the growth of the crops on them, for they will decay, and thus furnish carbonic acid to the young plants. But it cannot be imagined that their influence is very great, when it is considered that a good soil is manured only once every six or seven years, or once every eleven or twelve years, when sainfoin or lucerne has been raised on it, that the quantity of carbon thus given to the land corresponds to only 5'8 per cent, of what is removed in the form of herbs, straw, and grain ; and farther that the rain-water received by a soil contains much more car- bon in the form of carbonic acid than these vegetable constituents of the manure. The peculiar action then, of the solid ex- crements is limited to their inorganic con- stituents, which thus restore to a soil that which is removed in the form of corn, roots, or grain. When we manure land with the dung of the cow or sheep, we supply it with silicate of potash and some salts of phosphoric acid. In human faeces we give it the phosphates of lime and magnesia; and in those of the horse, phosphate of magnesia, and silicate of potash. In the straw which has served as litter, we add a farther quantity of silicate of potash and phosphates ; which, if the straw be putre- fied, are in exactly the same condition in which they were before being assimilated. It is evident, therefore, that the soil of a field will alter but little, if we collect and distribute the dung carefully ; a certain por- tion of the posphates, however, must be lost every year, being removed from the land * The details of the analysis are as follows: 2'356 grammes left 0'320 gramme ashes after incineration ; these consisted of the phosphate of lime and magnesia. 0'352 gramme yielded, on comoustion with oxide of copper, 0'576 gram, carbonic acid, and 0'218 gram, water. (L. P.) with the corn and cattle, and this portion will accumulate in the neighbourhood of large towns. The loss thus suffered must be compensated for in a well-managed farm, and this is partly done by allowing the fields to lie in grass. In Germany, it is considered that for every 100 acres of corn-land, there must, in order to effect a profitable cultiva- tion, be 20 acres of pasture-land, which pro- duce annually, on an average, 500 Ibs. of hay. Now, assuming that the ashes of the excrements of the animals fed with this hay amount to 6.82 per cent., then 341 Ibs. of the silicate of lime and posphates of magne- sia and lime must be yielded by these excre- ments, and will in a certain measure com- pensate for the loss which the corn-land had sustained. The absolute loss in the salts of phospho- ric acid, which are not again replaced, is spread over so great an extent of surface, that it scarcely deserves to be taken ac- count of. But the loss of phosphates is again replaced in the pastures by the ashes of the wood used in our houses for fuel. We could keep our fields in a constant state of fertility by replacing every year as much as we remove from them in the form of produce; but an increase of fertility, and consequent increase of crop, can only be obtained when we add more to them than we take away. It will be found, that of two fields placed under conditions otherwise similar, the one will be most fruitful upon which the plants are enabled to appropriate more easily and in greater abundance those contents of the soil which are essential to their growth and developement. From the foregoing remarks it will readily be inferred, that for animal excrements, other substances containing their essential constituents mav be substituted. In Flan- ders, the yearly loss of the necessaiy matters in the soil is completely restored by covering the fields with ashes of wood or bones, which may or may not have been lixiviated, and of which the greatest part consists of the phosphates of lime and magnesia. The great importance of manuring with ashes has been long recognised by agriculturists as the result of experience. So great a value, indeed, is attached to this material in the vicinity of Marburg and in the Wette- rau,* that it is transported as a manure from the distance of 18 or 24 miles. Its use will be at once perceived, when it is con- sidered that the ashes, after having been washed with water, contain silicate of pot ash exactly in the same proportion as in straw flO Si O 3 -f- K O.,) and that their only other constituents are salts of phospho- ric acid. But ashes obtained from various kinds of trees are of very unequal value for this pur- pose; those from oak-wood are the| least, * Two well-known agricultural districts ; the first in Hesse-Cassel, the second in Hesse-Darm- stadt. F AGRICULTURAL CHEMISTRY. and those from beech the most serviceable. The ashes of oak-wood contain only traces of phosphates, those of beech the fifth part of their weight, and those of the pine and fir from 9 to 15 per cent. The ashes of pines from Norway contain an exceedingly small quantity of phosphates, namely, only 1-8 per cent, of phosphoric acid. (Berthier.) With every 100 Ibs. of the lixiviated ashes of the beech which we spread over a soil, we furnish as much phosphates as 460 Ibs. of fresh human excrements could yield. Again, according to the analysis of De {Saussure, 100 parts of the ashes of the grain of wheat contain 32 parts of soluble, and 44-5 of insoluble phosphates, in all 76-5 parts. Now the ashes of wheat straw con- tain 11-5 per cent, of the same salts ; hence with every 100 Ibs. of the ashes of the beech, we supply a field with phosphoric acid suf- ficient for the production of 3820 Ibs. of straw (its ashes being calculated at 4-3 per cent., De Saussure,) or for 15-18000 Ibs. of corn, the ashes of which amount, according to De Saussure, to 1-3 per cent. Bone manure possesses a still greater im- portance in this respect. The primary sources from which the bones of animals are derived are, the hay, straw, or other substances which they take as food. Now, if we admit that bones contain 55 per cent, of the phosphates of lime and magne- sia (Berzelius,) and that hay contains as much of them as wheat-straw, it will follow that 8 Ibs. of bones contain as much phos- phate of lime as 1000 Ibs. of hay or wheat- straw, and 2 Ibs. of it as much as 1000 Ibs. of the grain of wheat or oats. These num- bers express pretty nearly the quantity of phosphates which a soil yields annually on the growth of hay and corn. Now the ma- nure of an acre of land with 40 Ibs. of bone dust is sufficient to supply three crops of wheat, clover, potatoes, turnips, &,c., with phosphates. But the form in which they are restored to a soil does not appear to be a matter of indifference. For the more finely the bones are reduced to powder, and the more intimately they are mixed with the soil, the more easily are they assimilated. The most easy and practical mode of effect- ing their division is to pour over the bones, in a state of fine powder, half of their weight of sulphuric acid diluted with three or four parts of water, and after they have been di- gested for some time, to add one hundred parts of water, and sprinkle this mixture over the field before the plough. In a few seconds, the free acids unite with the bases contained in the earth, and a neutral salt is formed in a very fine state of division. Ex- periments instituted on a soil formed from grauwacke, for the purpose of ascertaining the action of manure thus prepared, have distinctly shown that neither corn, nor kitchen-garden plants, suffer injurious ef- fects in consequence, but that on the con- trary they thrive with much more vigour. It has also been found that bones act more speedily and efficaciously after being boiled. This is probably owing to the re- moval of fatty matter, the presence of which impedes the putrefaction of the gelatin con- tained in them. In the manufactories of glue, many hun- dred tons of a solution of phosphates in mu- riatic acid are yearly thrown away as being useless. It would be important to examine whether this solution might not be substi- tuted for the bones. The free acid would combine with the alkalies in the soil, espe- cially with the lime, and a soluble salt would thus be produced, which is known to possess a favourable action upon the growth of plants. This salt, muriate of lime (or chloride of calcium,) is one of those compounds which attracts water from the atmosphere with great avidity, and in dry lands might advantageously supply the place of gypsum in decomposing carbonate of ammonia, with the formation of sal-am- moniac and carbonate of lime. A solution of bones in muriatic acid placed on land in autumn or in winter would, therefore, not only restore a necessary constituent of the soil, and attract moisture to it, but would also give it the power to retain all the am- monia which fell upon it dissolved in the rain during the period of six months.* The ashes of brown coal and peat often contain silicate of potash, so that it is evi- dent that these might completely replace one of the principal constituents of the dung of the cow and horse, and they contain also some phosphates. Indeed they are much esteemed in the Wetterau as manure for meadows and moist land. It is of much importance to the agricul- turist that he should not deceive himself re- specting the causes which give the peculiar action to the substances just mentioned. It is known that they possess a very favour- able influence on vegetation ; and it is like- wise certain that the cause of this is their containing a body, which, independently of the influence which it exerts by virtue of its form, porosity, and capability of attracting and retaining moisture, also assists in main- taining the vital processes in plants. If it be treated as an unfathomable mystery, the na- ture of this aid will never be known. In medicine, for many centuries, the mode of action of all remedies was supposed to be concealed by the mystic veil of Isis, but now these secrets have been explained in a * Immense quantities of bran are used in all print-works, for the purpose of clearing printed goods. After having served this purpose, it is thrown away. But the insoluble part of bran contains much phosphates of magnesia and soda ; it would, therefore, be useful to preserve it as a manure. This has been done for some years in a farm with which I am connected, and its value as a manure has been found so great that it is much preferred to cow-dung. In some works this waste bran is heaped up into little hillocks, which might be disposed of as a manure, instead of being an annoyance on account of the space which it occu- pies. ED. OF MANURE. 63 very simple manner. An unpoetical hand has pointed out the cause of the wonderful and apparently inexplicable healing virtues of the springs in Savoy, by which the inha- bitants cured their goitre ; it was shown that they contain small quantities of iodine. In burnt sponges used for the same purpose, the same element was also detected. The extraordinary efficacy of Peruvian bark was found to depend on a small quantity of a crystalline body existing in it, viz. quinine ; and the causes of the various effects of opium were detected in as many different ingredients of that drug. Calico-printers used for a long time the solid excrements of the cow, in order to brighten and fasten colours on cotton goods ; this material appeared quite indispensable, and its action was ascribed to a latent prin- ciple which it had obtained from the living organism. But since its action was known to depend on the phosphates contained in it, it has been completely replaced by a mix- ture of salts, in which the principal con- stituents are the phosphates of soda and lime.* Now all such actions depend on a definite cause, by ascertaining which we place the actions themselves at our command. It must be admitted as a principle of agri- culture, that those substances which have been removed from a soil must be com- pletely restored to it, and whether this resto- ration be effected by means of excrements, ashes, or bones, is in a great measure a mat- ter of indifference. A time will come when fields will be manured with a solution of glass, (silicate of potash,) with the ashes of burnt straw, and with salts of phosphoric acid, prepared in chemical manufactories, exactly as at present medicines are given for fever and goitre. There are some plants which require humus, and do not restore it to the soil by their excrements j whilst others can do with- out it altogether, and add humus to a soil which contains it in small quantity. Hence a rational system of agriculture would em- ploy all the humus at command for the su ply of the former, and not expend any of it for the latter ; and would in fact make use of them for supplying the others with humus. We have now considered all that is requi- site in a soil, in order to furnish its plants with the materials necessary for the forma- tion of the woody fibre, the grain, the roots, and the stem, and now proceed to the con- sideration of the most important object of agriculture, viz. the production of nitrogen in a form capable of assimilation the pro- duction, therefore, of substances containing * This mixture of salts is sold to calico-printers in large quantities under the name of " dung sub- stitute." It would be well worth experiment to try its effects as a manure upon land. Its cost is 3d. or 4d. per pound, and is not, therefore, dearer than nitrate of soda, which is now so extensively iwed. ED. this element. The leaves, which nourish the woody matter, the roots, from which the leaves are formed, and which prepare the substances for entering into the composition of the fruit, and, in short, every part of the organism of a plant, contain azotised matter in very varying proportions, but the seeds and roots are always particularly rich in them. Let us now examine in what manner the greatest possible production of substances containing nitrogen can be effected. Nature, by means of the atmosphere, furnishes ni- trogen to a plant in quantity sufficient for its normal growth. Now its growth must be considered as normal, when it produces a single seed capable of reproducing the same plant in the following year. Such a normal condition would suffice for the ex- istence of plants, and prevent their extinc- tion, but they do not exist for themselves alone; the greater number of animals de- pend on the vegetable world for food, and by a wise adjustment of nature, plants have the remarkable power of converting, to a certain degree, all the nitrogen offered to them into nutriment for animals. We may furnish a plant with carbonic acid, and all the materials which it may re- quire; we may supply it with humus in the most abundant quantity ; but it will not at- tain complete developement unless nitrogen is also afforded to it; an herb will be formed, but no grain ; even sugar and starch may be produced, but no gluten. But when we give a plant nitrogen in considerable quantity, we enable it to attract with greater energy from the atmosphere the carbon which is necessary for its nutri- tion, when that in the soil is not sufficient; we afford to it a means of fixing the carbon of the atmosphere in its organism. We cannot ascribe much of the powet of the excrements of black cattle, sheep, and horses, to the nitrogen which they con- tain, for its quantity is too minute. But that contained in the faeces of man is proportion- ably much greater, although by no means constant. In the faeces of the inhabitants of towns, for example, who feed on animal matter, there is much more of this consti- tuent than in those of peasants, or of such people as reside in the country. The faeces of those who live principally on bread and potatoes are similar in composition and pro- perties to those of animals. All excrements have in this respect a very variable and relative value. Thus those of black cattle and horses are of great use on soils consisting of lime and sand, which contain no silicate of potash and phosphates ; whilst their value is much less when applied to soils formed of argillaceous earth, basalt, granite, porphyry, clinkstone, and even mountain-limestone, because all these con- tain potash in considerable quantity. In such soils human excrements are extremely beneficial, and increase their fertility in a remarkable degree ; they are, of course, as 64 AGRICULTURAL CHEMISTRY. advantageous for other soils also; but for the manure of those first mentioned, the ex- crements of other animals are quite indis- pensable. OP URINE. We possess only one other natural source of manure which acts by its nitrogen, be- sides the faeces of animals, namely, the urine of man and animals. Urine is employed as manure either in the liquid state, or with the faeces which are impregnated with it. It is the urine contained in them which gives to the solid faeces the property of emitting ammonia, a property which they themselves possess only in a very slight degree. When we examine what substances we add to a soil by supplying it with urine, we find that this liquid contains in solution am- moriiacal salts, uric acid (a substance con- taining a large quantity of nitrogen,) and salts of phosphoric acid. According to Berzelius 1000 parts of hu- man urine contain : Urea 30'10 Free Lactic acid, Lactate of Ammonia, and animal matter not separable from them 17' 14 Uric acid I'OO Mucus of the bladder .... 0'32 Sulphate of Potash .... 3-71 Sulphate of Soda 3'16 Phosphate of Soda 2'94 Phosphate of Ammonia - - T65 Chloride of Sodium .... 4-45 Muriate of Ammonia 1'50 Phosphates of Magnesia and Lime TOO Silicious earth ..... 0'03 Water 933"00 100000 If we subtract from the above the urea, lactate of ammonia, free lactic acid, uric acid, the phosphate and muriate of ammo- nia; 1 per cent, of solid matter remains, consisting of inorganic salts, which must possess the same action when brought on a field, whether they are dissolved in water or in urine. Hence the powerful influence of urine must depend upon its other ingredients, namely, the urea and ammoniacal salts. The urea in human urine exists partly as lactate of urea, and partly in a free state. (Henry.) Now when urine is allowed to putrefy spontaneously, that is, to pass into that state in which it is used as manure, all the urea in combination with lactic acid is converted into lactate of ammonia, and that which was free, into volatile carbonate of ammonia. In dung-reservoirs well constructed and protected from evaporation, this carbonate of ammonia is retained in the state of solu- tion, and when the putrefied urine is spread over the land, a part of the ammonia will escape with the water which evaporates, but another portion will be absorbed by the soil, if it contains either alumina or iron; but in general only the muriate, phosphate, and lactate of ammonia remain in the ground. It is these alone, therefore, which enable the soil to exercise a direct influence on plants during the progress of their growth, and not a particle of them escapes being ab- sorbed by the roots. On account of the formation of this car- bonate of ammonia the urine becomes alka- line, although it is acid in its natural state. When it is lost by being volatilized in the air, which happens in most cases, the loss suffered is nearly equal to one half of the weight of the urine employed, so that if we fix it, that is, if we deprive it of its volatility, we increase its action two-fold. The exist- ence of carbonate of ammonia in putrefied urine long since suggested the manufacture of sal-ammoniac from this material. When the latter salt possessed a high price, this manufacture was even carried on by the farmer. For this purpose the liquid obtained from dunghills was placed in vessels of iron, and subjected to distillation; the product of this distillation was converted into muriate of ammonia by the common method. (De- machy.) But it is evident that such a thoughtless proceeding must be wholly re- linquished, since the nitrogen of 100 Ibs. of sal-ammoniac (which contains 26 parts of nitrogen) is equal to the quantity of nitrogen contained in 1200 Ibs. of the grain of wheat, 1480 Ibs. of that of barley, or 2755 Ibs. of hay. (Boussingault.) The carbonate of ammonia formed by the putrefaction of urine, can be fixed or de- prived of its volatility in many ways. If a field be strewed with gypsum, and then with putrefied urine or the drainings of dunghills, all the carbonate of ammonia will be converted into the sulphate which will remain in the soil. But there are still simpler means of effect- ing this purpose ; gypsum, chloride of cal- cium, sulphuric or muriatic acid, and super- phosphate of lime, are all substances of a very low price, and completely neutralise the urine, converting its ammonia into salts which possess no volatility. If a basin, filled with concentrated mu- riatic acid, is placed in a common necessary, so that its surface is in free communication with the vapours which rise from below, it becomes filled after a few days with crystals of muriate of ammonia. The ammonia, the presence of which the organs of smell amply testify, combines with the muriatic acid and loses entirely its volatility, and thick clouds or fumes of the salt newly formed hang over the basin. In stables the same may be seen. The ammonia that escapes in this manner is not only entirely lost, as far as our vegeta- tion is concerned, but it works also a slow, though not less certain destruction of the walls of the building. For when in contact with the lime of the mortar, it is converted into nitric acid, which gradually dissolves the lime. The injury thus done to a build- ing by the formation of the soltfble nitrates, has received (in Germany) a special name salpeterfrass. OF MANURE. 65 The ammonia emitted from stables and necessaries is always in combination with carbonic acid. Carbonate of ammonia and sulphate of lime (gypsum) cannot be brought together at common temperatures, without mutual decomposition. The ammonia enters into combination with the sulphuric acid, and the carbonic acid with the lime, form- ing compounds which are not volatile, and consequently destitute of all smell. Now, if we strew the floors of our stables, from lime to time, with common gypsum, ihey fcnll lose all their offensive smell, and none tf the ammonia which forms can be lost, out will be retained in a condition service- able as manure. With the exception of urea, uric acid contains more nitrogen than any other sub- stance generated by the living organism ; it is soluble in water, and can be thus absorbed by the roots of plants, and its nitrogen as- similated in the form of ammonia, and of the oxalate, hydrocyanate, or carbonate of ammonia. It would be extremely interesting to study the transformations which uric acid suffers in a living plant. For the purpose of experi- ment, the plant should be made to grow in charcoal powder previously heated to red- ness, and then mixed with pure uric acid. The examination of the juice of the plant, or of the component parts of the seed or fruit, would be a means of easily detecting the differences. NIGHT-SOIL. IN respect to the quantity of nitrogen con- tained in excrements, 100 parts of the urine of a healthy man are equal to 1300 parts of the fresh dung of a horse, according to the analyses of Macaire and Marcet, and to 600 parts of those of a cow. Hence it is evident that it would be of much importance to agriculture if none of the human urine were lost. The powerful effects of urine as a manure are well known in Flanders,* but they are considered invaluable by the Chi- nese, who are the oldest agricultural people we know. Indeed so much value is attached to the influence of human excrements by these people, that laws of the state forbid that any of them should be thrown away, and reservoirs are placed in every house, in which they are collected with the greatest care. No other kind of manure is used for their corn-fields.f * See the article " On the Agriculture of the Ketherlands," Journ. Royal Agri. Soc., vol. ii. part 1, page 43, for much interesting information Dn this subject. t Davis, in his History of China, states that very substance convertible into manure is dili- gently husbanded. ' ' The cakes that remain after the expression of their vegetable oils, horns and hoofs reduced to powder, together with soot and ashes, and the contents of common sewers, are much used. The plaster of old kitchens, which in China have no chimneys but an opening at the top, is much valued ; so that they will sometimes put a new plaster on a kitchen for the sake of the 9 China is the birth-place of the experi- mental art; the incessant striving after ex- periments has conducted the Chinese a thou- sand years since to discoveries, which have been the envy and admiration of Europeans for centuries, especially in regard to dying and painting, and to the manufactures of porcelain, silk, and colours for painters. These we were long unable to imitate, and yet they were discovered by them without the assistance of scientific principles ; for in the books of the Chinese we find recipes and directions for use, but never explana tions of processes. old." The ammonia contained in the fuel forms nitrate of lime with the lime in the mortar. " All sorts of hair are used as a manure, and barbers' shavings are carefully appropriated to that pur- pose. The annual produce must be considerable in a country where some hundred millions of heads are kept constantly shaved. Dung of all animals, but more especially night-soil, is esteemed above all others. Being sometimes formed into cakes, it is dried in the sun, and in fhis state be- comes an object of sale to farmers, who dilute it previous to use. They construct large cisterns or pits, lined with lime plaster, as well as earthen tubs, sunk into the ground, with straw over them to prevent evaporation, in which all kinds of vege- tables and animal refuse are collected. Thes* being diluted with a sufficient quantity of liquid, are left to undergo the putrefactive fermentation, and then applied to the land. In the case of every thing except rice, the Chinese seem to manure the plant itself rather than the soil, supplying it co- piously with their liquid preparation." " The Chinese husbandman," observes Sir G. Staunton, (Embassy, vol. ii.,) " always steeps the seeds he intends to sow in liquid manure, until they swell, and germination begins to appear, which'experience has taught him will have the effect of hastening the growth of plants, as well as of defending them against the insects hidden in the ground in which the seeds are sown. To the roots of plants and fruit-trees, the Chinese farmer applies liquid manure likewise." Lastly, we extract the following from a com- munication to Professor Webster, of Harvard College, United States: " Human urine is, if possible, more husbanded by the Chinese than night-soil for manure ; every farm, or patch of land for cultivation, has a tank, where all sub- stances convertible into manure are carefully de- posited, the whole made liquid by adding urine in the proportion required, and invariably applied in that state." This is exactly the process fol- lowed in the Netherlands. See Outlines of Flem- ish Husbandry, page 22. "The business of collecting urlrie and night- soil employs an immense number of persons, who deposit tubs in every house in the cities for the reception of the urine of the inmates, which ^es- sels are removed daily, with as much care as our farmers remove their honey from the hives." When we consider the immense value of night- soil as a manure, it is quite astounding that so little attention is paid to preserve it. The quantity is immense which is carried down by the drains in London to the River Thames, serving no other purpose than to pollute its waters. It has been shown, by a very simple calculation, that the value of the manure thus lost amounts annually to several millions of pounds sterling. A sub- stance, which by its putrefaction generates mias- mata, may, by artificial means, be rendered totally inoffensive, inodorous, and transportable, and yet prejudice prevents these means being resorted to. Eo. F 2 66 AGRICULTURAL CHEMISTRY. Half a century sufficed to Europeans not ily to equal but to surpass the Chinese in the arts and manufactures, and this was owing merely to the application of correct principles deduced from the study of che- mistry. But how infinitely inferior is the agriculture of Europe to that of China ! The Chinese are the most admirable gar- deners and trainers of plants, for each of which they understand how to prepare and apply the best-adapted manure. The agri- culture of their country is the most perfect in the world; and there, where the climate in the most fertile districts differs little from the European, very little value is attached to the excrements of animals. With us, thick books are written, but no experiments instituted ; the quantity of manure consumed by this and that plant is expressed in hun- dredth parts, and yet we know not what manure is ! If we admit that the liquid and solid ex- crements of man amount on an average to l Ib. daily, (f Ib. of urine and Ib. faeces,) and that both taken together contain 3 per cent, of nitrogen, then in one year they will amount to 547 Ibs., which contain 16-41 Ibs. of nitrogen, a quantity sufficient to yield the nitrogen of 800 Ibs. of wheat, rye, oats, or of 900 Ibs. of barley. (Boussingault.) This is much more than is necessary to add to an acre of land in order to obtain, with the assistance of the nitrogen absorbed from the atmosphere, the richest possible crop every year. Every town and farm might thus supply itself with the manure, which, besides containing the most nitrogen, contains also the most phosphates; and if rotation of the crops were adopted, they would be most abundant. By using, at the same time, bones and the lixiviated ashes of wood, the excrements of animals might be completely dispensed with. When human excrements are treated in a proper manner, so as to remove the mois- ture which they contain without permitting the escape of ammonia, they may be put into such a form as will allow them to be transported even to great distances. This is already attempted in many towns, and the preparation of night-soil for trans- portation constitutes not an unimportant branch of industry. But the manner in which this is done is the most injudicious which could be conceived. In Paris, for example, the excrements are preserved in the houses in open casks, from which they are collected and placed in deep pits at Montfaucon, but are not sold until they have attained a certain degree of dryness by eva- poration in the air. But whilst lying in the receptacles appropriated for them in the houses, the greatest part of their urea is converted into carbonate of ammonia ; lac- tate and phosphate of ammonia are also formed, and the vegetable matters contained in them putrefy ; all their sulphates are de- composed, whilst their sulphur forms sul- phuretted hydrogen and hydro-sulphate of ammonia. The mass, when dried by ex posure to the air, has lost more than half of the nitrogen which the excrements originally contained; for the ammonia escapes into the atmosphere along with the water which evaporates; and the residue now consists principally of phosphate of lime, with phos- phate and lactate of ammonia, and small quantities of urate of magnesia and fatly matter. Nevertheless it is still a very pow- erful manure, but its value as such would be twice or four times as great, if the excre- ments before being dried were neutralised with a cheap mineral acid. In other manufactories of manure the night-soil, whilst still soft, is mixed with the ashes of wood, or with earth, both of which substances contain a large quantity 01 caus- tic lime, by means of which a complete ex- pulsion of all its ammonia is effected, and it is completely deprived of smell. But such a residue applied as manure can act only by the phosphates which it still contains, for all the ammoniacal salts have been decom- posed and their ammonia expelled. The preparation of night-soil is now car- ried on in London to a considerable extent. Owing to the variable nature of the climate, artificial means are employed in its desicca- tion. The night-soil, after being subjected to one or other of the modes of treatment described below, is placed upon iron plates heated by means of furnaces. As soon as the night-soil is collected, it is placed in large broad trenches, until a suffi- cient quantity is accumulated for the pur- poses of the manufacturer. But here it undergoes the same process of putrefaction to which allusion has been made, and ac- quires a peculiarly offensive smell from the evolution of sulphuretted hydrogen and other gases, which are observed to escape. Unless some means be employed, at this stage of the process, to retain the ammonia, it escapes into the atmosphere in the form, of a carbonate. Various methods have been proposed to effect this purpose. Some manu- facturers mix the night-soil with chloride of lime, and evaporate off the water by the aid of heat. This possesses the advantage of depriving the excrements of smell, and at the same time partially fixes the ammonia which would otherwise escape. Chloride of lime always contains a considerable ex- cess of lime ; hence part of the ammonia contained in the night-soil is expelled by means of it. More simple and economical methods might be employed. A patent, which has been taken out for tbe preparation of this useful manure, states in its specification, that the night-soil is to be mixed with cal- cined mud and finely-divided charcoal. By this means, the smell is completely and in- 1 stantaneously removed, and the ammonia I retained by virtue of the affinity which alu- mina and charcoal exert for that compound. This plan is both simple and efficacious, but i the ammonia is apt to be expelled by the OF MANURE. 67 application of the heat employed in drying the manure. The addition of a cheap mine- ral acid to the night-soil, before admixture with these ingredients, would materially improve both of the above processes. It would no doubt be highly advantageous in the preparation of manures, to prepare them so that they contained all the ingredi- ents necessary for the supply of the plants to which they are applied. But these will of course vary according to the nature of the soils and plants for which they are in- tended. Thus bones, soap-boilers' waste, nitrate of soda, and ashes of wood, will often be found to form advantageous addi- tions. Sulphate of magnesia (Epsom salts) would, in most cases, form an invaluable ingredient in prepared night-soil. (See Sup- plementary Chapter on Soils.) The pro- ducts of the decomposition proceeding from the action of this salt upon night-soil are, sulphate of ammonia, phosphate of mag- nesia, and the double phosphate of magnesia and ammonia. Now all these salts exert a very favourable influence upon vegetation, and the phosphate of magnesia is, in many cases, perfectly indispensable to the growth and developement of certain plants. This suggestion is well worthy of the attention of the farmer. Perhaps the best and most practical me- thod of fixing the ammoniacal salts of urine and night-soil, is to mix them with the ashes of peat or coal. When the latter are employed, care must be taken to select such as are of a porous, earthy consistence. The ashes both of peat and coal contain in gene- ral magnesia ; hence their value as an in- gredient of prepared night-soil. When magnesia is not present, it will be necessary to add some magnesian limestone or Epsom salts. The night-soil should be mixed tho- roughly with the ashes, and exposed to the air to dry. The disagreeable smell is thus quickly removed, and a pulverulent manure obtained, which can be applied to the fields with facility. Animal charcoal, which has served for the discoloration of sugar, possesses the pro- perty of removing the offensive smell of night-soil, and is of itself an admirable ma- nure. In cases where it can be procured with facility, it will be found to add to the efficacy of the latter. GUANO. The sterile soils of the South American coasx are manured with a substance called guano, consisting of urate of ammonia and other ammoniacal salts, by the use of which a luxuriant vegetation and the richest crops are obtained. Guano has lately been im- ported in considerable quantity into Liver- pool and several other English ports, and is now experimentally employed as a manure by English agriculturists. A consideration of its composition and mode of action can- not, therefore, fail to be acceptable. Much speculation has arisen as to the true origin of guano,* but the most certain proof is now afforded, that it has been pro- duced by the accumulation of the excre- ments of innumerable sea-fowl which inhabit the islands upon which it is found. Meyen, the latest writer upon this subject, com- pletely coincides with this opinion ; for he saysf "Their number is Legion; they completely cloud the sun, when they rise from their resting-place in the morning in flocks of miles in length." Yet, notwith- standing their great number, thousands of years must have elapsed, before the excre- ments could have accumulated to such a thickness as they possess at present. Guano has been used by the Peruvians as a manure since the twelfth century ; and its value was considered so inestimable, that the govern- ment of the Incas issued a decree, by which capital punishment was inflicted upon any person found destroying the fowl on the Guano islands. Overseers were also ap- pointed over each province, for the purpose of insuring them further protection. Under this state of things, the accumulation of the excrements may have well taken place. All these regulations are, however, now aban- doned.:!: Rivero states that the annual con- sumption of guano for the purposes of agri- culture amounts to 40,000 fanegas. The increase of crops obtained by the use of guano is very remarkable. According to the same authority, the crop of potatoes is increased 45 times by means of it, and that of maize 35 times. The manner of apply- ing the manure is singular. Thus in Arica, where so much pepper ( Capsicum baccatum) is cultivated, each plant is manured three times : first upon the appearance of the roots, second upon that of the leaves, and lastly upon the formation of the fruit. (Humboldt.) From this it will be observed, that the Pe- ruvians follow the plan of the Chinese, in manuring the plant rather than the soil. The composition of guano points out how admirably it is fitted for a manure; for not only does it contain ammoniacal salts in abundance, but also those inorganic consti- tuents which are indispensable for the de- velopement of plants. The most recent analysis is that of Volc- kel, who found it to consist of Urate of Ammonia 9.0 Oxalate of Ammonia . . . 10.6 Oxalate of Lime ... 7.0 Phosphate of Ammonia . . . 6.0 Phosphate of Magnesia and Ammonia 2.6 Sulphate of Potash ... 5.5 Sulphate of Soda 3.8 Sal-ammoniac , 4.2 Phosphate of Lime 14.3 Clay and Sand : 4.7 * Much of the information regarding Guano here given is extracted from a paper in Liebig's Annalen, xxxvii. 3, 291. t Eeise urn die Erde, B. i. S. 434. $ Garcilaso, Historia de los Yncas, vol. i. p. 134. AGRICULTURAL CHEMISTRY. Organic substances not estimated, con-1 taining 12 per cent, of matter insolu- ^ ble in water- Soluable Salts of Iron f in small quantity. Water. . J 67.7 32.3 100.0 It will be observed from the above analy- sis, that urea does not enter into the compo- sition of guano. The uric acid of the ex- crements must have been decomposed into oxalic acid and ammonia. The soluble sub- stances contained in guano amount to half its weight. It is singular that we do not find nitrates amongst the ingredients which compose it. Guano possesses a urinous smell, precisely similar to that perceived on the evaporation of urine. The experiments upon the efficacy of this manure in Eng- land have not yet been sufficiently multi- plied to enable us to judge whether or not its virtues have been overrated. The corn-fields in China receive no other manure than human excrements. But we cover our fields every year with the seeds of weeds, which from their nature and form pass undigested along with the excrements through animals, without being deprived of their power of germination, and yet it is considered surprising that where they have once flourished, they cannot again be ex- pelled by all our endeavours : we think it very astonishing, while we really sow them ourselves every year. A famous botanist, attached to the Dutch embassy to China, could scarcely find a single plant on the corn-fields of the Chinese, except the corn itself.* The urine of horses contains less nitrogen and phosphates than that of man. Accord- ing to Fourcroy and Vauquelin it contains only five per cent, of solid matter, and in that quantity only 0.7 of urea; whilst 100 parts of the urine of man contain more than four times as much. The urine of a cow is particularly rich in salts of potash ; but according to Rouelle and Brande, it is almost destitute of salts of soda. The urine of swine contains a large quantity of the phosphate of magnesia and ammonia ; and hence it is that concretions of this salt are so frequently found in the urinary bladders of these animals. It is evident that if we place the solid or liquid excrements of man or the liquid ex- crements of animals on our land, in equal proportion to the quantity of nitrogen re- moved from it in the form of plants, the sum of this element in the soil must increase every year ; for to the quantity which we thus supply, another portion is added from the atmosphere. The nitrogen which we export as corn and cattle, and which is thus absorbed by large towns, serves only to be- nefit other farms, if we do not replace it. A farm which possesses no pastures, and not fields sufficient for the cultivation of fodder, * Ingenhouss on the Nutrition of Plants, page 129 (German edition. 7 requires manure containing nitrogen to be imported from elsewhere, if it is desired to produce a full crop. In large farms, the an- nual expenditure of nitrogen is completely replaced by means of the pastures. The only absolute loss of nitrogen, there- fore, is limited to the quantity which man carries with him to his grave ; but this at the utmost cannot amount to more than 3 Ibs. for every individual, and is being col- lected during his whole life. Nor is this quantity lost to plants, for it escapes into the atmosphere as ammonia during the pu- trefaction and decay of the body. A high degree of culture requires an in- creased supply of manure. With the abun- dance of the manure, the produce in corn and cattle will augment, but must diminish with its deficiency. From the preceding remarks it must be evident, that the greatest value should be at- tached to the liquid excrements of man and animals,, when a manure is desired which shall supply nitrogen to the soil. The greatest part of a superabundant crop, or, in other words, the increase of growth which is in our power, can be obtained ex- clusively by their means. When it is considered that with every pound of ammonia which evaporates a loss of 60 Ibs. of corn is sustained, and that with every pound of urine a pound of wheat might be produced, the indifference with which these liquid excrements are regarded is quite incomprehensible. In most place? only the solid excrements impregnated with the liquid are used, and the dunghills con- taining them are protected neither from eva- poration nor from rain. The solid excre- ments contain the insoluble, the liquid all the soluble phosphates, and the latter con- tain likewise all the potash which existed as organic salts in the plants consumed by the animals. Fresh bones, wool, hair, hoofs, and horn, are manures containing nitrogen as well as phosphates, and are consequently fit to aid the process of vegetable life. All animal matter is fitted for the same purpose. Butchers' offal, such as the blood and intes- tines of animals, form a most powerful ma- nure. It is in general necessary to dilute such manure by admixture with other kinds less powerful in their action. One hundred parts of dry bones contain from 32 to 33 per cent, of dry gelatine ; now supposing this to contain the same quantity of nitrogen as animal glue, viz., 5.28 per cent., then 100 parts of bones must be con- sidered as equivalent to 250 parts of human urine. Bones may be preserved unchanged for thousands of years, in dry or even in moist soils, provided the excess of rain is prevent- ed ; as is exemplified by the bones of an- tediluvian animals found in loam or gyp- sum, the interior parts being protected by the exterior from the action of water. But they become warm when reduced to a fine OF MANURE. 69 powder, and moistened bones generate heat and enter into putrefaction ; the gelatine which they contain is decomposed, and its nkrogen converted into carbonate of ammo- nia and other ammoniacal salts, which are retained in a great measure by the powder itself. ("Bones burnt till quite white, and re- cently heated to redness, absorb 7.5 times their volume of pure ammoniacal gas.) ARTIFICIAL MANURES. WE have now examined the action of the animal or natural manures upon plants; but it is evident that if artificial manures con- tain the same constituents, they will exer- cise a similar action upon the plants to which they are applied. We shall only notice here one or two of those principally employed. Since it has been ascertained that animal manures act (as far as the formation of or- gaaic matter is concerned^ only by the am- monia which they contain, attention has been devoted by chemists to discover a more economical means of presenting this ammonia to plants. The water which dis- tils from the retorts in the preparation of coal gas is strongly charged with this alkali, but is at the same time mixed with tar and other empyreumatic impurities. It has been customary to allow the tarry matter to sub- side, and decant off the clear, supernatant liquor. This liquor, being diluted to such a degree as to be tasteless, is applied as a manure to the field. Now, the ammoniacal liquor of the gas- works contains the ammonia in the form of carbonate and hydro-sulphate of ammonia (sulphuret of ammonium). The latter com- pound is a deadly poison to vegetables, nor can we conceive that by dilution its proper- ties can be changed. The carbonate of ammonia is volatile, and escapes into the at- mosphene. To obviate this latter inconveni- ence and render it more transportable, it has been proposed to convert the carbonate into the sulphate, by means of gypsum.* But this does not remove the hydro-sulphate. A more simple and efficacious method is to add a solution of sulphate of iron (the green vitriol of the shops) to the liquor, until no further precipitation ensues. Sulphuret and carbonate of iron are thus formed, and the whole of the ammonia enters into com- bination with the sulphuric acid, and forms sulphate of ammonia. Care must be taken to avoid too great an excess of sulphate of iron ; and the liquor thus prepared should be freely exposed to the air to promote the oxidation. The liquor still, however, contains em- pvreumatic matters, which are injurious to plants. These may be removed by evapo- rating the liquor to dryness, and heating the residue to incipient redness. By this means they are rendered insoluble, and the sul- * Three Lectures on Agriculture, by Dr. Dau- beny, page 87. phate of ammonia is not affected, unless the heat has been carried too far. The liquor properly diluted has been found very advan- tageous, even without the removal of the empyreumatic matter. Nitrate of soda has lately engaged much attention, and is supposed to exert its fa- vourable action upon vegetation by yielding nitrogen to those of their constituents which contain it. The experiments which have hitherto been instituted with this manure do not warrant us in concluding with positive certainty that it is the nitrogen alone to which it owes its efficacy, but they certainly render this a plausible explanation of its virtues. Thus Mr. Pusey, the late able president of the Royal Agricultural Society, has shown, that the same effects are pro- duced by putrefied urine, soot, gas-liquor, and nitrate of soda.* Now the three for- mer act by virtue of the ammonia which enters into their composition. The usual effects produced by these and nitrate of soda are to increase the intensity of the green colouring matter, to augment the quantity of straw, but to produce a light grain. Mr. Hyettf has communicated the results of an analysis of two samples of wheat grown under similar circumstances, one of which had been treated with nitre, the other not. The former contained 23*25 per cent, or gluten, and 1-375 of albumen; the latter only 19 per cent, of gluten, and O62 of al- bumen. Here the azotised matters appear to have considerably increased in quantity. There is nothing opposed to the supposition that nitric acid may be decomposed by plants, and its nitrogen assimilated. We find that vegetables possess the power of decomposing carbonic acid, and of appro- priating its carbon for their own use. Now this acid is infinitely more difficult to decom- pose than nitric acid. But there are other circumstances which oppose the adoption of the view that nitrate of soda acts by vir- tue of the nitrogen which enters into its composition. Were this the case, the ac- tion should be more uniform than it has hitherto been found to be. On some soils the salt does not possess the smallest influ- ence ; whilst on others it affords great bene- fit. We can only furnish an explanation of this seeming caprice by a reference to the chemical composition 01 the soil upon which it is applied. If the advantages attending the application of nitrate of soda are due to the alkaline base which it contains, then it is evident that this manure can be of small value on soils containing a quantity of alka- lies sufficient for the purposes of the plants grown upon them; whilst, on the other hand, such as are deficient in these must ex- perience benefit through its means.:}: In * Journal of the Royal Agricultural Society, vol. ii., p. 123. t Journal of the Royal Agricultural Society, vol. ii., p. 143. I General Sir Howard Elphinstone informs mi that he found carbonate of soda (soda ash) an ex 70 AGRICULTURAL CHEMISTRY certain cases in which nitrate of soda has failed, nitrate of potash (common saltpetre) has been very successful. Analyses of wheat grown with nitrate of soda and nitrate of potash would be of interest, in order to determine whether a mutual substitution of their respective bases is effected. It is to be hoped that future experiments will throw more light upon the action of this interest- ing manure, for theory cannot be satisfied with those already existing. It has been usual to employ a less quantity by weight of nitrate of potash than of nitrate of soda. This procedure seems rather empirical, for unless sanctioned by experience, it would d priori appear to be better to add the great- est quantity of that salt which possesses the highest equivalent. Now the equivalent of nitrate of potash is considerably higher than that of nitrate of soda. Charcoal in a state of powder must be considered as a very powerful means of pro- moting the growth of plants on heavy soils, and particularly on such as consist of ar- gillaceous earth.* Ingenhouss proposed dilute sulphuric acid as a means of increasing the fertility of a soil. Now, when this acid is sprinkled on calcareous soils, gypsum (sulphate of lime) is immediately formed, which of course prevents the necessity of manuring the soils with this material. 100 parts of concen- trated sulphuric acid diluted with from 800 to 1000 parts of water, are equivalent to 176 parts of gypsum. SUPPLEMENTARY CHAPTER. *N THE CHEMICAL CONSTITUENTS OF SOILS. THE fertility of a soil is much influenced by its physical properties, such as its poro- sity, colour, attraction for moisture, or state of disintegration. But independently of these conditions, the fertility depends upon the chemical constituents of which the soil is composed. We have already shown, at considerable length, that those alkalies, earths, and phos- phates, which constitute the ashes of plants, are perfectly indispensable for their deve- lopement ; and that plants cannot nourish upon soils from which these compounds are absent. The necessity of alkalies for the vital processes of plants will be obvious, when we consider that almost all the differ- cellent manure for his land. The crops obtained by means of it presented the same general charac- ters as those manured with nitrate of potash, and exhibited a greater intensity of colour. If this is found uniformly to be the case, it will very much etrengthen the supposition that the action of ni- trate of soda is due to its alkaline constituent. ED. * For much valuable information on the sub- ject of manures, see " Agricultural Chemistry," vol. viii. of Sir H. Davy's collected Works. ent families of plants are distinguished by containing certain acids, differing very mucu in composition ; and further, that these acids do not exist in the juice in an isolated state, but generally in combination with certain alkaline or earthy bases. The juice of the vine contains tartaric acid, that of the sorrel oxalic acid. It is quite obvious that a pecu- liar action must be in operation in the or- ganism of the vine and sorrel, by means of which the generation of tartaric and oxalic acid is effected ; and also that the same ac- tion must exist in all plants of the same genus. A similar cause forces corn-plants to extract silicic acid from the soil. The number of acids found in different plants is very numerous, but the most common are those which we have already mentioned ; to which may be added acetic, malic, citric, aconitic, maleic, kinovic acids, &,c. When we observe that the proper acids of each family of plants are never absent from it, we must admit that the plants be- longing to that family could not attain per- fection, if the generation of their peculiar acids were prevented. Hence, if the pro- duction of tartaric acid in the vine were ren- dered impossible, it could not produce grapes, or in other words, would not fructify. Now the generation of organic acids is pre- vented in the vine, and, indeed, in all plants which yield nourishment to men and ani- mals, when alkalies are absent from the soil in which they grow. The organic acids in plants are very rarely found in a free state ; in general, they are in combination with potash, soda, lime, or magnesia. Thus, silicic acid is found as silicate of potash, acetic acid as acetate of potash or soda, oxalic acid as oxalate of potash, soda, or lime, tartaric acid as bitartrate of potash, &.c. The potash, soda, lime, and magnesia in these plants are, therefore, as indispensa- ble for their existence as the carbon from which their organic acids are produced. In order not to form an erroneous conclu- sion regarding the processes of vegetable nutrition, it must be admitted that plants re- quire certain salts for the sustenance of their vital functions, the acids of which salts exist either in the soil (such as silicic or phos- phoric acids) or are generated from nutri- ment derived from the atmosphere. Hence, if these salts are not contained in the soil, or if the bases necessary -for their production be absent, they cannot be formed, or in other words, plants cannot grow in such a soil. The juice, fruit, and leaves of a plant can- not attain maturity, if the constituents ne- cessary for their formation are wanting, and salts must be viewed as such. These salts do not, however, occur simultaneously in all plants. Thus, in saline plants, soda is the only alkali found; in corn plants, lime and potash form constituents. Several con- tain both soda and potash, some both potash and lime ; whilst others contain potash and magnesia. The acids vary in a similar manner. Thus one plant may contain CONSTITUENTS OF SOILS. 71 phosphate of lime, a second, phosphate of | gether lost to the English agriculturist. In magnesia, a third, an alkali combined with large towns it is either allowed to run into silicic acid, and a fourth, an alkali in com- bination with a vegetable acid. The re- spective quantities of the salts required by plants are very unequal. The aptitude of a soil to produce one, but not another kind of plant, is due to the presence of a base which the former requires, and the absence of that, indispensable for the developernent of the latter. Upon the correct knowledge of the bases and salts requisite for the sustenance of each plant, and of the composition of the soil upon which it grows, depends the whole system of a rational theory of agri- culture; and that knowledge alone can ex- plain the process of fallow, or furnish us with the most advantageous methods of af- fording plants their proper nourishment. Give so says the rational theory to one plant such substances as are necessary for its developement, but spare those, which are not requisite, for the production of other plants that require them. It is the same with regard to these bases as it is with the water which is necessary for the roots of various plants. Thus, whilst one plant flourishes luxuriantly in an arid soil, a second requires much moisture, a third finds necessary this moisture at the cpmmencement of its developement, and a fourth (such as potatoes) after the appear- ance of the blossom. It would be very er- the rivers, or sink into the ground in such a manner as to be of no benefit to the vegeta- ble kingdom. The most important growth in England is that of wheat ; then of barley, oats, beans, and turnips. Potatoes are only cultivated to a great extent in certain localities ; rye, beet-root, and rape-seed, not very generally. Lucerne is only known in a few districts, whilst red clover is found universally. Now, the selection of inorganic manures for these plants may be fixed upon by an examina- tion of the composition of their ashes. Thus wheat must be cultivated in a soil rich in silicate of potash. If this soil is formed from feldspar, mica, basalt, clinkstone, or indeed of any minerals which disintegrate with facility, crops of wheat and barley may be grown upon it for many centuries in suc- cession. But, in order to support an unin- terrupted succession, the annual disintegra- tion must be sufficiently great to render solutle a quantity of silicate of potash suf- ficient for the supply of a full crop of wheat or barley. If this is not the case, the soil must either be allowed to lie fallow from time to time, or plants may be cultivated upon it which contain little silicate of pot- ash, or the roots of which are enabled to penetrate deeper into the soil than corn plants in search of this salt. During this roneous to present the same quantity of i interval of repose, the materials of the soil water to all plants indiscriminately. Yet this obvious principle is lost sight of in the manuring of plants. An empirical system of agriculture has administered the same kind of manures to all plants ; or when a selection has been made, it was not based upon a knowledge of their peculiar charac- ters or composition. The cost of labour in England has given rise to the production of much ingenuity in the invention of machines, which have pro- duced improvements in the mode of appli- cation of manures. In order to use these with advantage, pulverulent manures are employed, instead of the common stable manure, which is generally mixed with much straw. The necessity for such forms of manure disintegrate, and potash in a soluble state is liberated on the layers exposed to the action of the atmosphere. When this has taken place, rich crops of wheat may be again expected. The alkaline phosphates, as well as the phosphates of magnesia and lime, are ne- cessary for the production of all corn-plants. Now, bones contain the latter, but none of the former salts. These must, therefore, be furnished by means of night-soil, or of urine, a manure which is particularly rich in them.* Wood ashes have been found very useful for wheat in calcareous soils ; for these ashes contain both phosphate of lime and silicate of potash. In like manner stable manure and night-soil render clayey soils fertile, by furnishing the magnesia in naturally suggested the employment of bone ! which they are deficient. The ashes of all dust, dried dung, lime, ashes, &c. Now, although by these means the necessary phosphates are furnished to a soil, and solid animal excrements rendered unnecessary, they have led to the neglect of the liquid excrements, that is, of the urine of men and animals, which is thus completely lost to agriculture. For although the meadows receive, during autumn and winter, when cattle are fed upon them, the solid and liquid excrements of these animals, yet the urine of man, into which all the nitrogenous con- stituents of animals are finally deposited, is completely lost to the fields. This most im- portant of all manures, so properly estimated in Flanuers, Germany, and China, is alto- kinds of herbs and decayed straw are capable of replacing wood ashes. A compost manure, which is adapted to furnish all the inorganic matters to wheat, oats, and barley, may be made, by mixing equal parts of bone dust and a solution of silicate of potash (known as soluble glass in commerce,) allowing this mixture to dry in the air, and then adding 10 or 12 parts ot fypsum, with 16 parts of common salt, uch a compost would render unnecessary * It has been already stated that bran phosphate of soda and phosphate of magnesia, so that it is useful as a manure where phosphates are desired. ED. AGRICULTURAL CHEMISTRY. the animal manures, which act by their in- organic ingredients. According to Berthier, 100 parts of the ashes of wheat straw con- tain Of matter soluble in water - - 9'0 Of matter insoluble in water - 81*0 Now 100 parts of the soluble matter con- tain Carbonic acid - - a trace Sulphuric acid - - 2'0 Muriatic acid 13'0 Silica - - '- 35 '0 Potash and Soda - - - 50.0 100-0 100 parts of the insoluble matter contain Carbonic acid ... Phosphoric acid * - 1'2 Silica .... 75.0 Lime - - - - - 5'8 Oxide of Iron and Charcoal lO'O Potash - - 8'0 100.0 The silicate of potash employed in the preparation of the compost described above must not deliquesce on exposure to the air, but must give a gelatinous consistence to the water in which it is dissolved, and dry to a white powder by exposure. It is only attractive of moisture when an excess of potash is present, which is apt to exert an injurious influence upon the tender roots of plants. In those cases where silicate of potash cannot be procured, a sufficiency of wood ashes will supply its place.* All culinary vegetables, but particularly the cruciferae, such as mustard, (sinapis alba and nigra,) contain sulphur in notable quantity. The same is the case with turnips, the different varieties of rape, cabbage, celery, and red clover. These plants thrive best in soils containing sulphates ; hence if these salts do not form natural constituents of the soil, they must be introduced as ma- nure. Sulphate of ammonia is the best salt for this purpose. It is most easily pro- cured by the addition of gypsum or sulphate of ironf (green vitriol) to putrefied urine. * In some parts of the grand- duchy of Hesse, where wood is scarce and dear, it is customary for the common people to club together and build baking ovens, which are heated with straw instead of wood. The ashes of this straw are carefully collected and sold every year at very high prices. The farmers there have found by experience that the ashes of straw form the very best manure for wheat ; although it exerts no influence on the growth of fallow-crops (potatoes or the legumi- nosae, for example.) The stem of wheat grown in this way possesses an uncommon strength. The cause of the favourable action of these ash.es will be apparent, when it is considered that all corn-plants require silicate of potash ; and that the ashes of straw consist almost entirely of this compound. ED. t If sulphate of iron be employed, it ought not to be added in great excess, and the urine must be exposed to the air for some time after, for the purpose of converting the iron into the peroxide. A salt of the protoxide of iron is injurious to vegetation. | Horn, wool, and hoofs of cattle, contain ! sulphur as a constituent, so that they will be found a valuable manure when adminis tered with sojuble phosphates, (with urine, for example.) Phosphate of magnesia and ammonia forms the principal inorganic constituent of the potato ; salts of potash also exist in it, but in very limited quantity. Now the soil is rendered unfitted for its cultivation, even though the herb be returned to it after the removal of the crop, unless some means are adopted to replace the phosphate of magnesia removed in the bulbous roots. This is best effected by mixtures of night-soil with bran, magnesian limestone, or the ashes of certain kinds of coal. I applied to a field of pota- toes manure, consisting of night-soil and sulphate of magnesia, (Epsom salts,) and obtained a remarkably large crop. The ma- nure was prepared by adding a quantity oi sulphate of magnesia to a mixture of urine and fasces, and mixing the whole with the ashes of coal or vegetable mould, till it ac- quired the consistence of a thick paste, which was thus dried by exposure to the sun. It has been formerly mentioned, that the secondary and tertiary limestones contain potash : marl, and the calcareous minerals used for the preparation of hydraulic mortar, may be particularly specified. These have been found to form excellent manures for heavy clayey soils, particularly for such as disintegrate with difficulty. They are most efficacious when burnt, but can only be ap- plied in this state after harvest, and ought to be ploughed into the soil as quickly as possible. By the action of lime upon clay, the potash contained in the latter is rendered soluble. This may easily be shown by mix- ing one part of marl with half its weight of burned lime, adding water, and setting aside the mixture to repose for some time. Even after a space of 24 hours, an appreciable quantity of potash may be detected in the water.* A most striking proof of the influence of potash upon vegetation has been furnished by the investigations of the " administration" of tobacco in Paris. For many years accu- rate analyses of the ashes of various sorts of tobacco have been executed, by the orders of the " administration ;" and it has been found, as the result of these, that the value of the tobacco stands in a certain relation to * One of the causes of the advantages produced by subsoil ploughing is, that it exposes the soil to the disintegrating influences of the atmosphere. Hence it is that the subsoil plough is so beneficial in siliceous soils, and exerts no apparent effect upon those which contain much clay. The former disintegrate and liberate their potash both with facility and rapidity ; whilst the disintegration of the latter proceeds with slowness, and no appre- ciable effects are produced. (See Journal of the Agricultural Society, vol. ii., p. 27.) It is proba- ble, however, that if the land received a dressing of lime after subsoil ploughing, the effects would be produced more rapidly. ED. COMPOSITION OP SOILS. 73 ihe quantity of potash contained in the ashes. By this means a mode was furnished o f distinguishing the different soils upon which the tobacco under examination had been cultivated, as well as the peculiar class to which it belonged. Another striking fact was also disclosed through these analyses. Certain celebrated kinds of American tobacco were found gradually to yield a smaller quantity of ashes, and their value dimi- nished in the same proportion. For this in- formation I am indebted to M. Pelouze, pro- fessor of the Polytechnic School in Paris. There are certain plants which contain either no potash, or mere traces of it. Such are the poppy,, (papaver somniferum,') which generates in "its organism a vegetable alka- loid, Indian corn, (zea wwn/s,)and helianthus tuberosm. For plants such as these the pot- ash in the soil is of no use, and farmers are well aware that they can be cultivated with- out rotation on the same soil, particularly when the herbs and straw, or their ashes, are returned to the soil after the reaping of the crop. One cause of the favourable action of the nitrates of soda and potash must doubtless be, that through their agency the alkalies which are deficient in a soil are furnished to it. Thus it has been found that in soils de- ficient in potash, the nitrates of soda or pot- ash have been very advantageous; whilst those, on the other hand, which contain a sufficiency of alkalies, have experienced no beneficial effects through their means. In the application of manures to soils we should be guided by the general composition of the ashes of plants, whilst the manure applied to a particular plant ought to be selected with reference to the substances which it demands for its nourishment. In general, a manure should contain a large quantity of alkaline salts, a considerable proportion of phosphate of magnesia, and a smaller pro- portion of phosphate of lime ; azotised ma- nure and ammoniacal salts cannot be too frequently employed. In the following part of this chapter I shall describe a number of analyses of soils executed by Sprengel, together with obser- vations on their sterility and fertility, as stated by that distinguished agriculturist. It is unnecessary to describe the modus ope- ramli used in the analyses of these soils, for this kind of research will never be made by farmers, who must apply to the professional chemist, if they wish for information regard- ing the composition ol their soils. Under the term surface-soil, we mean that portion of soil which is on the surface ; whilst by subsoil we mean that which is be- low the former, and out of the reach of the ordinary plough. CHEMICAL COMPOSITION OF CERTAIN SOILS ACCORDING TO ANALYSIS. 1. Surface-soil (A) a good loamy soil .*rom the vicinity of Gandersheim. It is re- 10 markable for producing uncommonly fine red clover when manured with gypsum. B) is an analysis of the subsoil. 1 00 parts ontain : (A) (B) Silica, with fine siliceous sand - 91-331 93'883 Alumina - - - - - 1'344 T944 3 eroxide of iron, with a little pro- toxide - 1-562 2-226 eroxide of manganese 0'032 0.320 Magnesia and silica, in combina- tion with sulphuric acid and humus ..... 0-800 0720 Vlagnesia, with silica and humic acid combined - - 0'440 0'340 otash, in combination with silica 0'156 0'105 Soda, principally in combination with silica, and a little as com- mon salt - 0-066 0-060 Phosphoric acid - - - 0'098 0190 Sulphuric acid in combination with lime - - - - 0-111 0'012 hlorine (in common salt) - 012 0'012 Humus, with traces of azotised matter 4'100 0'184 100-000 100-000 An inspection of the above analyses will show that the soil contains a very small pro- portion of salts of sulphuric acid a circum- stance which accounts for the favourable action of gypsum upon it. 2. The surface-soil (A) is a fine-grained loamy soil from Gandersheim, distinguished for the remarkably large crops of beans, peas, tares, &.C., which it produces when manured with gypsum. (B) is the analysis of the subsoil. 100 parts contain : (A) (B) Silica, with fine siliceous sand - 90-221 92'324 Alumina - - - - 2-106 2'262 Peroxide and protoxide of iron - 3'951 2'914 Peroxide of manganese - - 0'960 Lime, principally combined with phosphoric acid and humus - 0'539 2-960 0-533 Magnesia, with silicate of pot- ash, &c. 0-730 0-340 Potash 0-067 0'304 Soda - 0-010 a trace Phosphoric acid ' * - 0'367 0'122 Sulphuric acid (in gypsum) - a trace 0*010 Chlorine (in common salt) - O'lOO 0'004 Humus and azotised matter - 0.900 Loss .... 0-140 0-228 100-000 lOO'OOO The analysis of this soil shows, that, with the exception of gypsum, every ingredient is present which is requisite for the nourish- ment of leguminous plants. Hence it is that gypsum exerts such a favourable influ- ence upon it. 3. Surface-soil (A) a strong loamy sand, from Brunswick. (B) the analysis of the subsoil. 100 parts contain : lA) Silica, with coarse siliceous sand 95 -698 Alumina Peroxide and protoxide of iron 2'496 Peroxide of manganese - a trace Lime - - - 0'038 Magnesia - - 0'147 Potash and soda, the greatest part in combination with silica 0'090 Phosphate of iron - 0'164 G (B) 96-880 0-890 1-496 a trace 0.019 0-260 0-079 0-110 74 AGRICULTURAL CHEMISTRY. Sulphuric acid (in gypsum) Chlorine (in common salt) Humus (A) (B) - 0-007 a trace - O'OIO a trace - 0-846 0-226 100-000 100-000 This soil was much improved by manur- ing with lime and ashes. It was then found well fitted for clover, beans, and peas. 4. Surface-soil (A) a loamy sand, from the environs of Brunswick. (B) analysis of the subsoil at the depth of 3 feet. 100 parts contain: (A) (B) Silica and fine siliceous sand - 94'724 97'340 Alumina - T638 0.806 Protoxide and peroxide of iron with manganese ' - - T960 1-201 Lime - - 1'028 0'296 Magnesia - - - a trace 0'095 Potash and soda - - 0'077 0'112 Phosphoric acid - - 0'024 0'015 Gypsum - - - O'OIO a trace Chlorine of the salt 0207 a trace Humus - - - 0-512 0-135 100-000 100-000 This soil produces luxuriant crops of lu- cerne and sainfoin, as well as of all other plants the roots of which penetrate deeply into the ground. The reason is apparent. The subsoil contains magnesia, which is wanting in the surface-soil. 5. Surface-soil (A) a loamy sand, from the environs of Brunswick. (B) analysis of the subsoil at a depth of 2 feet. 100 parts contain : (A) (B) 95-843 95.180 0-600 1-600 1-800 2-200 a trace a trace 0-038 0.455 0-006 0-160 0-005 0-004 0-198 0-400 0-002 a trace 0-006 0-001 1-000 . . . 0-502 . . . 100-000 100-000 This soil is characterised by its great sterility. White clover could not be made to grow upon it. The obvious cause of its poverty is a deficiency of lime, magnesia, potash, and gypsum; for we find that the fertility of the soil was much increased by manuring it with marl. The white clover, which formerly had refused to grow on this soil, now grew upon it with much luxuri- ance. The aridity of the soil could not have been the cause of its sterility, for the stiff nature of the subsoil on which it rested pre- vented a deficiency of moisture. 6. Surface-soil (A) a loamy land from the environs of Brunswick. (B) the analysis of the subsoil, at a depth of 2 feet. 100 parts contain : Silica, with coarse siliceous sand Alumina - Protoxide and peroxide of iron Peroxide of manganese - Lime, in combination with silica Magnesia in do. do. Potash and soda Phosphate of iron - Sulphuric acid - Chlorine ... Humus soluble in alkalies Humus insoluble in alkalies CA) (B) Silica, with fine siliceous sand - 94'998 96.490 Alumina - - - 0'610 1'083 Protoxide and peroxide of iron T080 1.472 Peroxide of manganese - 0.268 0'400 Lime, in combination with silica 0'141 0.182 Magnesia, idem - - 0'208 0'205 Potash, idem - - - O'OSO 0'070 Soda, idem - - 0'044 O'OSO Phosphate of iron - - 086 0'030 Gypsum - - - 0'041 O'OOS Common salt - - - 0'004 0'003 Humus soluble in alkalies - 0'400 O'OIO Humus accompanied by azotised matter - - - 2.070 . . . Resinous matter a trace . . . 100.000 100.000 This soil is by no means remarkable for its sterility, but is decidedly improved by manuring with burned ferruginous loam. It is, however, rendered still better by the use of burned marl a manure which is rich in iron, potash, gypsum, and phosphate of lime. The marl does not exert so favour- able an action when applied in its natural state; but the heat liberates the potash from the insoluble compound which it forms with silica. 7. Surface-soil (A) a loamy sand, from Brunswick. (B) analysis of the subsoil at a depth of 1^ feet. 100 parts contain: Silica, with fine siliceous sand Alumina - Protoxide and peroxide of iron Peroxide of manganese Lime, combined with silica Magnesia, idem Potash, idem Soda, idem ... Phosphate of iron Sulphuric acid contained in gyp- sum ... Chlorine - Humus soluble in alkalies - Humus, with azotised organic remains - (A) (B) 92-980 96-414 0-820 1-000 1-666 1-370 0.188 0-240 0'748 0-364 0-168 0-160 0-065 0-045 0-130 0082 0246 0-043 a trace O'OOS a trace 0'007 0-764 0-270 2-225 ' ' ' 100-000 100-000 The soil when manured with gypsum is very favourable to the production of legu- minous plants and red clover. But it is very remarkable, on account of the rust which always attacks the corn plants which may be grown upon it. This rust and mil- dew (uredo linearis, puccinia graminis) is a disease which attacks the stem and leaves, and is quite different from the brand (uredo glumarum) which appears on the seeds and organs of reproduction. Rust is most fre- quently detected on plants growing on soils which contain bog-ore or turf-iron ore. Ac- cording to Sprengel, rust contains phosphate of iron, to which this chemist ascribes the origin of the disease. It is very possible that other causes may operate in the production of similar diseases. 8. Soil, a fine-grained loamy marl, from the vicinity of Schoningen. It produces corn, which is, however, very liable to blight. 100 parts contain : COMPOSITION OF SOILS. Silica, with siliceous sand Alumina .... Protoxide and peroxide of iron - Peroxide of manganese Lime (principally carbonate) Magnesia, idem ... Potash, with silica Soda with silica - - Phosphate of iron Sulphuric acid with rime Carbonic acid, with lime and magnesia Humus soluble in alkalies Humus - 93-870 1-248 1-418 0-360 0-546 0-560 0-050 0-040 0'246 0-027 1-145 0-400 0090 100-000 It will be observed that a considerable quantity of phosphate of iron is contained in this soil, and the corn which grows upon it is, as in the former case, dispose*! to rust. 9. Surface-soil (A) a loamy soil, from Brunswick, remarkable on account of pro- ducing buck-wheat, which is exceedingly poor in the grain. (B) analysis of the sub- soil at a depth of 1^ foot. 100 parts con- tain: (A) (B) Silica, with coarse siliceous sand 95'114 92'458 Alumina - - - 1'OSO 2'530 Protoxide and peroxide of iron 1'900 2'502 Protoxide and peroxide of man- ganese - - - 0'320 0'920 Lime, in combination with silica 0'380 0710 Magnesia, idem - - 0*300 0'551 Potash, with silica 0'020 0.120 Soda - - - 0.004 0"034 Phosphate of iron - - 0'052 0'175 Sulphuric acid with lime - 0'006 a trace Chlorine (in common salt) 005 a trace Humus soluble in alkalies 0'619 * ' Humus - - 0-200 . 100-000 100-000 By manuring the land with wood ashes, the soil is enabled to produce buck-wheat, with rich grain ; the leguminous plants also thrive luxuriantly upon it. This increased fertility is due to the ashes, by means of which both potash and phosphates are sup- plied to the land. 10. Subsoil of a loamy, sandy soil, from Brunswick. It is remarkable for having produced excellent crops of hops for a long series of years. 100 parts, by weight, con- sist of: Silica, with siliceous sand Alumina ..... Protoxide and peroxide of iron Peroxide of manganese Lime, in combination with silica Magnesia .... Potash - Soda - Phosphoric acid ... Sulphuric acid - Chlorine - ... Humus soluble in alkalies - Humus - .... 95.660 1.586 1.616 0.240 0.083 0.080 0.030 0.220 0.039 0.003 a trace 0.080 0.360 100.000 Aitnough the hops contain a large quan- tity of potash, soda, phosphoric acid, sul- phuric acid, lime, and magnesia, yet we do not find that these exist in the soil in super- abundant quantity. Nor is it necessary that they should, for the roots of the hops pene- trate 8 or 10 feet deep into the soil, and search out the materials fitted to nourish the plants. Hence it is that hops thrive well on soils comparatively poor in their proper ingredi- ents. The same is the case with all plants of a similar nature, the roots of which pos- sess a tendency to extend in search of food ; we see this particularly in lucerne and sain- foin. SOILS OF HEATHS. 11. Soil of a heath converted into arable land, in the vicinity of Brunswick. It is naturally sterile, but produces good crops when manured with lime, marl, cow-dung, or the ashes of the heaths which grow upon it. Silica, and coarse siliceous sand - - 71.504 Alumina 0.780 Protoxide and peroxide of iron, principally combined with humus ... 0.420 Peroxide of manganese, idem - 0.220 Lime, idem 0.134 Magnesia, idem - ... 0.032 Potash and soda, principally as silicates - 0.058 Phosphoric acid, (principally as phosphate of iron) - - - - - 0.115 Sulphuric acid (in gypsum) - 0.018 Chlorine (in common salt) ... 0.014 Humus soluble in alkalies - 9.820 Humus, with vegetable remains - 14.975 Resinous matters - - 1.910 100.000 Ashes of the soil of the heath, before be- ing converted into arable land : Silica, with siliceous sand ... 92.641 Alumina ..... 1.352 Oxides of iron and manganese - - 2.324 Lime, in combination with sulphuric and phosphoric acids .... 0.929 Magnesia, combined with sulphuric acid - 0.283 Potash and soda (principally as sulphates and phosphates .... 0.564 Phosphoric acid, combined with lime - 0.250 Sulphuric acid, with potash, soda and lime 1.620 Chlorine in common salt - - 0.037 100.000 12. Surface-soil of a fine-grained loam, from the vicinity of Brunswick. It is re- markable from the circumstance, that not a single year passes in which corn plants are cultivated upon it without the stem of the plants being attacked by rust. Even the grain is covered with a yellow rust, and is much shrunk. 100 parts of the soil con- tain : Silica and fine siliceous sand - - 87.859 Alumina ...... 2.652 Peroxide of iron with a large proportion of protoxide .... 5.132 Protoxide and peroxide of manganese - 0.840 Lime principally combined with silica 1.459 Magnesia, idem 0.280 Potash and soda, idem ... 0.090 Phosphoric acid in combination with iron 0.505 Sulphuric acid in combination with lime 0.068 Chlorine in common salt ... 0.006 Humus 1.109 100.000 76 AGRICULTURAL CHEMISTRY. This soil does not suffer from want of drainage : it is well exposed to the sun, is in an elevated situation, and in a good state of cultivation. In order to ascertain whether the rust was due to the constituents of the soil, (phosphate of iron?) or to certain for- tuitous circumstances unconnected with their operation, a portion of the land was removed to another locality, and made into an artificial soil of fifteen inches in depth. Upon this barley and wheat were sown ; but it was found, as in the former case, that the plants were attacked by rust, whilst barley growing on the land surrounding this soil was not at all affected by the disease. From this experiment it follows, that certain con- stituents in the soil favour the developement of rust. 13. Soil of a heath, which had been brought into cultivation in the vicinity of Brunswick. The analysis was made before any kind of crops had been grown upon it. Corn-plants were first reared upon the new soil, but were found to be attacked by the rust, even on those parts which had been manured respectively with lime, marl, pot- ash, wood ashes, bone-dust, ashes of the heath plant, common salt and ammonia. 100 parts contain : Silica with coarse siliceous sand - 51'337 Alumina - - - - - - 0' 528 Protoxide and peroxide of iron in combina- tion with phosphoric and humic acids 0*398 Protoxide and peroxide of manganese - 0'005 Lime in combination wJ'h humus 0'230 Magnesia idem ... 0*040 Potash and soda O'OIO Phosphoric acid - 0'066 Sulphuric acid .... Q'022 Chlorine ...... 0*014 Humus soluble in alkalies - 13*210 Resinous matters ... 2*040 Coal of humus and water - 32*100 100*000 The next analysis represents the soil after being burnt. 100 parts by weight of the soil left after ignition only 50 parts. 100 parts of these ashes consisted of: Silica and siliceous sand ... 95-204 Alumina : 1'640 Peroxide of iron ..... 1 344 Peroxide of manganese . - - O'OSO Lime in combination with sulphuric acid 0'544 Magnesia combined with silica 0"465 Potasli and soda .... Q'052 Phosphoric acid (principally as phosphate of iron ...... 0-330 Sulphuric acid 0'322 Chlorine - - 0019 lOO'OOO By comparing this analysis with the one which has preceded it, an increase in cer- tain of the constituents is observed, particu- larly with respect to the sulphuric acid, pot- ash, soda, magnesia, oxide of iron, oxide of manganese, and alumina. From this it fol- lows, that the humus, or in other words, the vegetable remains, must have contained a quantity of these substances confined within it, in such a manner that they were not ex- hibited by analysis. Oats and barley were sown on this land the second year after being reclaimed, and both suffered much from rust, although dif- ferent parts of the soil were manured with marl, lime and peat-ashes; whilst other por- tions were left without manure. In the first year, all the different parts of the field pro- duced potatoes, but they succeeded best in those divisions which had been manured with peat-ashes, lime and marl. In the second year, oats mixed with a little barley were sown upon the soil; and the straw was found to be strongest on the parts treated with peat-ashes, lime, marl, and ashes of wood. Red clover was sown on the third year; it appeared in best condition on those portions of the soil manured with marl and lime. Upon the divisions of the field which had been left without manure, as well as on those manured with bone-dust, potash, am- monia and common salt, the clover scarcely appeared above ground. The divisions of the field, which had been manured in the first year with peat-ashes, ammonia, and ashes of wood, were sown with buckwheat after the removal of the first crop of clover. The buckwheat succeeded very well on all tho divisions, yet a marked difference was perceptible in favour of the portion treated with ammonia. These experiments show us, that a dressing of lime did not completely remove from the soil its tendency to impart rust to the plants grown upon it. Never- theless it is highly probable, that as soon as the protoxide of iron became converted into the peroxide by exposure to the atmosphere, lime would possess more power in decom- posing the phosphate of iron. 14. Subsoil of a loamy soil in the vicinity of Brunswick. It is remarkable from the circumstance that sainfoin cannot be culti- vated upon it more than two or three years in succession. The portion analysed was taken from a depth of five feet. 100 parts contained : Silica with very fine siliceous sand Alumina ..... Peroxide of iron .... Protoxide of iron - Protoxide and peroxide of manganese Lime Magnesia ..... Potash and soda .... Phosphoric acid, combined with iron 90-035 1-976 4-700 1-115 0-240 0-022 0-115 0-300 0-098 Sulphuric acid (the greatest part in combina- tion with protoxide of iron) - - 1-399 Chlorine - ... a trace 100.000 Now the results of the analysis give a suffi- cient account of the failure of the sainfoin. The soil contains above one per cent, of sulphate of protoxide of iron (green vitriol of commerce,) a salt which exerts a poison- ous action upon plants. Lime is not pre- sent in quantity sufficient to decompose this salt. Hence it is that sainfoin will not thrive on this soil, nor indeed lucerne, or any other CONSTITUENTS OF SOILS. 77 of the plants with deep roots. The evil can- not be obviated by any methods sufficiently economical for the farmer, because the soil cannot be mixed with lime at a depth of five or six feet. For many years experiments have been made in vain, in order to adapt this soil for sainfoin and lucerne,, and much expense incurred, which could all have been saved, had the soil been previously analysed. This example affords a most convincing proof of the importance of chemical know- ledge to an agriculturist. 15. Surface soil (A) of a sandy loam in the vicinity of Brunswick, celebrated for its beautiful crops of clover, rye, potatoes, and barley. The clover must, however, always be manured with gypsum. (B) is an ana- lysis of the subsoil at the depth of 1 foot. 100 parts contain: (A) (B) Silica with coarse siliceous sand 94 -274 95*146 Alumina .... 1.560 T416 Peroxide of iron with a little phosphoric acid - - - 2'496 2'528 Peroxide of manganese - 0'240 0'320 Lime 0'400 0'297 Magnesia : 0'230 0221 Potash and soda - - - 0'102 0'060 Sulphuric acid - - - 039 0'012 Chlorine .... O'OOS a trace Humus soluble in alkaline car- bonates - - - 0-444 . . Humus .... 0210 . . 100-000 100-000 The best property of this soil is, that its inferior layers are nearly of the same com- position as the superior, as far as the inor- ganic constituents are concerned. It is a soil upon which the plants mentioned above will seldom fail ; and as it possesses a very good mixture to the depth of four or five feet, it would, doubtless, produce lucerne also. 16. Surface-soil (A) of a sandy loam in the vicinity of Brunswick. It produces ex- cellent crops of oats and clover, when the latter is manured with gypsum. (B) Ana- lysis of the subsoil taken from a depth of 1^ foot. 100 parts contain: (A) (B) Silica and siliceous sand 94'430 89-660 Alumina .... 1-474 0'980 Peroxide of iron with a little phosphoric acid - - 2'370 7*616 Peroxide of manganese - a trace a trace Lime, principally combined with silica .... 0-680 0'954 Magnesia, idem - - . 0'290 0'520 Potash. .... 0-190? n ., n Soda 0-0105 15 Sulphuric acid ... a trace a trace Chlorine .... 0-015 a trace Humus .... 0-541 0-120 100-000 100000 Both the surface and the sub-soil contain only traces of sulphuric acid. Hence the ipplication of gypsiim is attended with great benefit. Without doubt, marl and lime would be found of essential service. 17. Soil from the environs of Brunswick, consisting principally of sand, and eminently remarked for its sterility. It was, however, much improved by manuring it with marl which contained 24 per cent, of lime, to- gether with magnesia, manganese, potash, soda, gypsum, and common salt. 100 parts of the soil contained : Silica and siliceous sand Alumina ..... Protoxide and peroxide of iron Peroxide of manganese Lime in combination with silica Magnesia, idem ... Potash Soda Phosphoric acid combined with iron Sulphuric acid .... Chlorine - * Humus - - ... - 95-841 0-600 1-800 a trace 0-038 0-006 0-002 0003 0-198 0-002 0-006 1-504 100-000 Here another proof is presented, that a soil may be very rich in humus and yet be very poor as regards fertility. By means of the marl, the inorganic ingredients of the plants are furnished to the soil, which con- tains them in very small quantity. 18. The soil of a very fertile loam from the vicinity of Walkenried. 100 parts con- tain : Silica, with coarse-grained silicious sand 88-456 Alumina 0'650 Peroxide and protoxide of iron, accompanied by much magnetic iron sand Peroxide of manganese ... Carbonate of lime - Carbonate of magnesia ... Potash combined with silica Soda combined with silica Phosphate of lime - Sulphate of lime .... Common salt Humus soluble in alkalies ... Humus with several azotised organic re- mains 5-608 0-560 1-063 1-689 0-04* 0-012 0-035 a trace 0'X>5 0550 1-333 100-000 Gypsum acts most excellently upon this land. The soils in the southern range of the Harz mountains are particularly re- marked for containing more magnesia than lime. Even the different varieties of marl contain a considerable quantity of magnesia. Thus, in a specimen of marl obtained from the vicinity of Walkenried, I obtained 55 per cent, carbonate of lime, and 30 per cent, carbonate of magnesia . in another 41 per cent, lime, and 1 1 per cent, magnesia ; and in a third, 47 per cent, lime, and 13J per cent, magnesia. Most of these soils contain also 1 per cent, of gypsum, and 1 per cent, phosphate of lime, and are, therefore, well fitted for manuring other lands. 19. Subsoil of a loam from a depth of 1$ foot. It occurs in the vicinity of Brunswick. The surface-soil is remarkable on account of producing beautiful red clover on being manured with gypsum; although the soil itself contains only traces of lime, magnesia, potash, and phosphoric acid. 100 parts of the subsoil contained : o2 78 AGRICULTURAL CHEMISTRY. Silica and coarse siliceous sand 88'980 ! Alumina ..... 2*240 Protoxide and peroxide of iron 3'840 Peroxide of manganese - - a trace Carbonate of lime - - - 2'720 Carbonate of magnesia ... 0'600 Potash and soda 0'095 Phosphate of lime ... 1.510 Sulphate of lime a trace Common salt .... 0"015 100-000 At a greater depth than the subsoil of which the analysis is here given, the soil passes into marl,, which contains 20^ per cent, of carbonate of lime. The sulphuric acid deficient in the soil was supplied by means of the gypsum. SOILS IN THE KINGDOM OF HANOVER. 20. (A) Analysis of a barren heath-soil from Aurich in Ostfriesland *, (B) a sandy soil containing much humus but also sterile (C) a sandy soil possessing the same cha- racters as B. 100 parts contained : (A) (B) (C) Silica and coarse siliceous sand - - 95-778 85*973 96721 Alumina - - 0*320 0*320 0*370 Protoxide and peroxide of iron - 0-400 0-440 0*480 Peroxide of manganese a trace a trace a trace Lime - - 0*286 0.160 0*005 Magnesia - - 0'060 0'240 0*080 Soda - - 0-036 0*012 0'036 Potash - - a trace a trace a trace Phosphoric acid a trace a trace a trace Sulphuric acid - a trace a trace a trace Chlorine in common salt 0-052 0*019 0'058 Humus - . 0-768 0'636 O'SOO Vegetable remains 2'300 8-200 1-450 100-000 100-000 100-000 21. Analysis of the clayey subsoil of a moor, which, after being burned, is used as a manure to the above soils, A, B, C. 100 parts contain : Silica and siliceous sand - - 87'219 Alumina .... 4*200 Peroxide of iron with a little phosphoric acid 5'200 Peroxide of manganese - - 0*310 Lime .... 0*320 Magnesia .... 0*380 Potash principally combined with silica 0*130 Soda principally combined with silica - 0*274 Sulphuric acid combined with lime, magne- sia, and potash ... 0*965 Chlorine .... 0"002 Humus ..... I'OOO 100*000 By comparing this analysis with that of the three soils which have preceded, it will be observed that this subsoil is fitted to im- part to them those mineral ingredients in which they are deficient. 22. Surface soil of a barren heath in the vicinity of Walsrode in Luneberg. 100 parts by weight contain : Silica and siliceous sand ... 92*216 Alumina .... 0*266 Peroxide of iron .... 942 Protoxide of iron - - - 0'394 Peroxide of manganese - - - a trace Lime, in combination with silica, sulphuric acid, and humus - T653 Magnesia, in combination with silica 0'036 Potash, principally in combination with silica 0'038 Soda - ... Phosphoric acid - - - - Sulphuric acid ... Chlorine - - - - - Humus, soluble in alkaline carbonates Humus ..... Resinous matter ... 100-000 This soil contains a large quantity of protoxide of iron, which, together with a deficiency of phosphoric acid, is the cause of its sterility. But when this land was manured with the ashes of peat, it was rendered much more fertile. The ashes used for this purpose were found to contain in 100 parts : Silica, with siliceous sand - - 96-352 Alumina - - 1*859 Peroxide and protoxide of iron, with a lit- tle phosphoric acid 1*120 Peroxide of manganese ... 0'160 Lime - - - - 0*112 Magnesia - - - -0*141 Potash - - - - 0-093 Soda 0.007 Sulphuric acid - - - 0-152 Chlorine 0*004 100-000 The ashes, on exposure to the air, ab- sorbed ammonia. 23. Analysis of a very fertile loamy soil from Gottingen. It is very rich in humus, and produces beautiful crops of peas, beans, lucerne, and beet. The sieve separates from 100 parts of the soil : Small stones, principally limestone - 1 Quarzy sand, with a little magnetic iron sand 15 Earthy part ... 84 100 100 parts of the soil, freed from stones, consists of: Silica, and fine siliceous sand - - 83*298 Alumina, combined with silica - 1*413 Alumina, partly in combination with humus 3715 Peroxide and protoxide of iron, in combi- nation with silica ... 0.724 Peroxide and protoxide of iron, partly free and partly in combination with humus 2-244 Peroxide and protoxide of manganese 0.280 Lime, with coal of humus, sulphur, and phosphoric acid - - 1*814 Magnesia, combined with silica - 0'422 Magnesia, combined with humus - 0'400 Potash . 0-003 Soda 0*001 Phosphoric acid - - 0*166 Sulphuric acid - - - - 0*069 Chlorine .... 0002 Carbonic acid (as carbonate of lime) - 0*440 Humus, soluble in alkalies - 0*789 Humus, with a little wa|er - 3*250 .Nitrogenous matter ... 0*960 Resinous matter ... a trace 100-000 The subsoil is of the same composition as CONSTITUENTS OF SOILS. 79 the surface, with this difference only, that it contains more potash, soda, and chlorine,,* and is interspersed with fragments of fresh- water shells. Hejice it is that the soil pro- duces the deep-rooted plants in such luxu- riance. 24. Soil of a sterile moor, which had been burned three times, and upon which buckwheat had been cultivated. 100 parts contained : Humus, soluble in alkalies - 9'250 Vegetable remains, charcoal, quarzy sand, and earthy particles - 90*750 100-000 100 parts by weight left, after ignition, 10 parts of ashes. 100 parts of these ashes consisted of: Silica and siliceous sand Alumina - Peroxide of iron Peroxide of manganese Carbonate of lime Carbonate of magnesia Potash .... Soda .... Phosphoric acid Sulphate of lime (gypsum) - Chlorine ... - 79-600 6-288 - 0-857 0-400 - 7-652 1-640 0-080 0-028 - 0215 3-235 - 0-005 100-000 Soils such as this, after having been burned several times, and made to produce buckwheat, are completely deprived of their potash and soda ; and in consequence of this are rendered quite barren. Hence it is that ashes of wood exert such an astonish- ing effect upon them. 25. Analysis of a very fertile loamy sand, from Osnabriick, near Rotherfeld. It is re- markable for being manured only once every 10 or 12 years, and bears beautiful wheat as the last crop. 100 parts contain: Silica, with coarse siliceous sand Alumina .... Peroxide and protoxide of iron, with a little phosphoric acid ... Peroxide of manganese Carbonic acid, and a little phosphate of lime ..... Carbonate of magnesia Potash and soda Phosphoric acid ... Sulphuric acid ... Chlorine - ... Humus, soluble in alkaline carbonates - Humus - ... Nitrogenous matter ... 86-200 2000 2-900 o-ioo 4-160 0-520 0-035 0-020 0-021 o-oio 0-544 3-370 0-120 lOO'OOO The soil in question lies on the southern exposure of a hill, which consists of layers of limestone and marl. The rain-water penetrates through these layers, and becomes saturated with the soluble salts contained in them, such as potash, gypsum, common * The portion of the suface-soil subjected to analysis was taken from the field after long-con- tinued rain. Hence the small quantity of salts of |K>tasli and soda. salt, lime, magnesia, and saltpetre. It after- wards reaches the soil, and manures it with these ingredients. It is only in this manner that we are enabled to explain the fertility of this soil ; for, reasoning from its chemical composition, we would be induced, a priori, to suppose that it would be barren. At the base of this hill, certain portions of the land are covered with calcareous tuff, containing the above salts : a fact which proves that the water which penetrates through the soil must also contain them in solution. The large proportion of humus exhibited by the analysis depends upon the nature of the manure to which it was treated. 26. Analysis of a heavy alluvial soil, from Norden. 100 parts contain : Silica, and very fine siliceous sand Alumina .... Peroxide of iron ... Peroxide of manganese Lime .... * Magnesia .... Potash ..... Soda, in combination with silica Phosphoric acid, in combination with lime ..... Sulphuric acid ... Chlorine ... Humus, soluble in alkalies - Humus and nitrogenous matter 84-543 3-458 3-488 0-560 0-319 0-740 a trace 6-004 0-260 0-008 0-008 0-416 0-196 100-000 The portion of the soil subjected to analy- sis was taken at a depth of 10 inches, from a field which had received no manure for several years. It had previously produced in succession barley, beans, wheat, and grass, the latter for two years. The soil is remarkable, in a chemical point of view, from the large quantity of soda which it contains. Although the sulphuric acid, chlorine, and potash are present in small quantity, yet this does not present any bar- rier to the developement of the plants, as the surface-soil is 18 inches in depth. 27. Analysis of a heavy alluvial soil in the vicinity of Norden. 100 parts contain : Silica, and very fine siliceous sand Alumina - Peroxide of iron Peroxide of manganese - Carbonate of lime Carbonate of magnesia Potash, in combination with silica Soda, idem .... Phosphoric acid Sulphuric acid Chlorine ... Humus, soluble in alkalies Humus with nitrogenous matter 79-174 3-016 4-960 0-600 2-171 2-226 0-025 6-349 0-534 a trace 0-005 0782 0-158 100-000 The specimen for analysis was taken at a depth of 10 inches from the surface of a field, which had been manured five years previously, and had produced since that time rape, rye, wheat, and beans. The crops of all these were plentiful, and of excellent quality. It is singular that this soil, which contains such a small proportion of gypsum, 80 AGRICULTURAL CHEMISTRY. should be adapted for the cultivation of beans, and must be ascribed to the depth of the surface-soil. Yet, notwithstanding this, gypsum would form a beneficial manure to the land. 28. Analysis of a very fertile alluvial soil, from Honigpolderj no manure had ever been applied to it. 100 parts contain : Siliceous sand separated by the sieve - 4'5 Earthy portion of the soil - 95'5 100.0 100 parts of the latter consisted of: Silica, and fine siliceous sand Alumina .... Peroxide of iron ... Peroxide of manganese Lime - Magnesia .... Potash, principally in combination with silica - Soda, idem ' - P hosphoric acid combined with lime Sulphuric acid, idem Chlorine (in common salt) Carbonic acid, combined with lime Humus soluble in alkalies Humus - - - Nitrogenous matter - Water - - .... . lOO'OOO Corn has been cultivated for seventy years upon this soil, which has never received dung or any other kind of manure it is, however, occasionally fallowed. The sub- soil retains the same composition as the surface-soil for a depth of 612 feet, so that it may be considered inexhaustible. When one portion of the soil is rendered unfitted for use, the inferior layers are brought up to the surface. 29. Analysis of a soil from Rahdingen, near Balje. In this case the sea has assisted in the formation of the soil. The field yielded beautiful corn after being manured with stable dung, being particularly re- marked for its fine crops of wheat, beans, and winter barley. 100 parts contain : Silica, siliceous sand, and silicates* Alumina - - Peroxide of iron .... Peroxide of manganese Lime - Magnesia Potash and soda soluble in water Phosphoric acid .... Sulphuric acid .... Chlorine (in common salt) Humus, soluble in alkaline carbonates Humus Nitrogenous matter ... Water ...... 87-012 4-941 2-430 0-192 0-292 0-145 0-005 0-114 0-07 0.003 0-658 2-666 1-412 0-042 100'00( 30. Soil of a field remarkable fc: produ cing large crops of hemp and horse-radish 100 parts consisted of: Silica and siliceous sand Alumina Peroxide of iron - 84.02 - 4-496 - 5-12 eroxide of manganese ,ime ..... lagnesia 'otash oda ...... Hosphoric acid - - - - ulphuricacid Chlorine lumus soluble in alkaline carbonates lumus and nitrogenous matter 2-OdO 0'942 1-740 0-050 0-012 0-482 0-012 0-008 0-897 0-138 100-000 31. Surface-soil of a field near Bracken- urg; it produces very bad red clover. 100 arts contain: lilica, with very fine siliceous sand - 92-014 Alumina 2'652 eroxide of iron ..... 3'192 eroxide of manganese - - - - 0'480 ,ime 0-243 Vlagnesia 0'700 otash combined with silica - - 0.125 soda, idem - - - . - - 0-026 hosphoric acid, in combination with lime 0*078 Sulphuric acid - - - - -a trace hlorine ^ a trace lumus and nitrogenous matter - - O'lSO Jumus soluble in alkaline carbonates - 0*340 100.000 The cause that clover will not flourish on his soil is probably due to the deficiency of rypsum and common salt. 32. Surface-soil of a field near Padding- )uttel. This field is particularly adapted or the growth of red clover. 1 00 parts con- sist of: Silica and siliceous sand - 93 '720 Alumina 1'740 Peroxide of iron - - - - - 2'060 Peroxide of manganese .... 0'320 Lime 0'121 Magnesia 0'700 Potash, principally in combination with silica 0'062 Soda, idem .... Phosphoric acid .... Sulphuric acid .... Chlorine (in common salt) Humus soluble in alkaline carbonates Humus with nitrogenous matter 0-109 - 0-103 - 0-005 - 0-050 - 0-890 - 0-120 100-000 SOILS IN BOHEMIA. 33. Surface-soil of a very fertile field in the province of Dobrawitz and Lautschin. 100 parts gave Siliceous sand, with much magnetic iron sand 4*286 Earthy part separated by the sieve - -95714 100-000 An aqueous infusion of the soil contained gypsum, common salt, magnesia, and hu- mus. 100 parts of the soil gave : Silica 89-175 Alumina 2 '652 Protoxide and peroxide of iron - - 3'136 Peroxide of manganese - - - - 0*320 Lime 1*200 Magnesia T040 Potash, in combination with silica 0'075 Soda, idem (principally) - - - 0"354 Phosphoric acid, in combination with lime 0'377 CONSTITUENTS OP SOILS. 81 Sulphuric acid, idem Chlorine (in common salt) Humus soluble in alkalies Humus ... Nitrogenous matter - 0*081 0'920 0-456 0-208 100-000 34. Surface-soil of a very fertile field in the province of Dobrawitz and Lautschin. 100 parts of the earth consisted of: Siliceous sand, with a little magnetic iron sand 43-780 Finer part separated by the sieve - 56-220 100-000 100 parts yielded to water 0-175 part of salts, consisting of common salt, gypsum, magnesia, and humic acid. 100 parts, by weight, of the earth consisted of: Silica 89-634 Alumina - - 3'224 Protoxide and peroxide of iron - - 2-944 Peroxide of manganese - - - 1 160 Lime 0'349 Magnesia 0'300 Potash in combination with silica - - 0'160 Soda, idem 0'428 Phosphoric acid, in combination with lime 0'246 Sulphuric acid, idem .... 005 Chlorine (in common salt) - - 0'012 Humus soluble in alkalies ... 0750 Humus - 0-340 Nitrogenous matter - 0'448 100-000 35. Analysis of a soil formed by the dis- integration of basalt. 100 parts of the earth consisted of: Siliceous sand, with very much magnetic iron sand ..... 8'428 Earthy portion of the soil - - 91 '572 100-000 The aqueous infusion of the earth con- tained only traces of common salt and gyp- sum, with humus, lime, and magnesia. 100 parts consisted of: Silica * - - - - - - 83-642 Alumina - - '* ' ' - '- -- - 3'978 Protoxide and peroxide of iron - - 5312 Peroxide of manganese - - . 0'960 Lime 1'976 Magnesia --;.-. 0*650 Potash, in combination with silica - 0*080 Soda, idem ------ 0'145 Phosphoric acid, in combination with lime 0*273 Sulphuric acid, idem .... Humus soluble in alkaline carbonates Chlorine Humus ..... Nitrogenous matter ... a trace - 1-270 a trace 0-234 1-480 100-000 Manure consisting of gypsum, common salt, or ashes of wood, would be highly con- ducive to the fertility of this land. SOILS IN THE " MARKGRAFSCHAFT MAHREN." 36. Surface-soil of a field very remarka- ble for its fertility. The field is called Haargraben, and is situated near the village of Nebstein. It has never been manured or allowed to lie fallow, and yet has produced for the last 160 years the most beautiful crops ; thus furnishing a remarkable exam- ple of unimpaired fertility. lOO'OOO parts of this soil consisted of: Course and fine siliceous sand, with a little magnetic iron sand - - 35'400 Earthy matter 64-600 100-000 100 parts of the earth yielded to water 0-010 sulphuric acid, 0-010 chlorine, 0-007 soda, 0-012 magnesia, 0-010 potash, with a little silica, humus, und nitrogenous matter, but no appreciable trace of nitrates. 100 parts of the soil contained : Silica 77-209 Alumina ...... 8*514 Peroxide of iron ..... 6'592 Peroxide of manganese ... i -530 Lime - - - - - - 0'927 Magnesia 1*160 Potash, principally in combination with silica 0'140 Soda, idem 0*640 Phosphoric acid, combined with lime and iron 0'651 Sulphuric acid, combined with lime - O'Oll Chlorine (in common salt) ... O'OIO Humus soluble in alkalies ... 0'978 Humus 540 Nitrogenous matter - - - - T108 100-000 It is apparent from the above analysis that, notwithstanding the long period during which this land has been cultivated without manure, it still remains very rich in matters adapted for the nutrition of plants. SOILS IN HUNGARY. 37. Analysis of a very fertile soil from Esakang. 100 parts of the earth con- tained : ., -.-A- Very fine siKcedus sand Earthy matter - 2-820 97-180 100-000 The aqifeous decoction of the soil contained principally gypsum, common salt, silica, magnesia, and humus. 100 parts of the soil yielded : Silica Alumina - - Peroxide and protoxide of iron Peroxide of manganese Carbonate of lime .... Carbonate of magnesia Potash combined with silica Soda combined with silica Phosphoric acid, combined with lime Sulphuric acid .... Chlorine in common salt Humus soluble in alkalies Humus - Nitrogenous organic matter - 76-038 4-654 6-112 0-900 3-771 4-066 0-030 T379 0546 0021 0015 1-160 1-100 0-208 100-000 Subsoil of the same field at a depth of two feet. 100 parts consist of: 82 AGRICULTURAL CHEMISTRY. Very fine tfjceous sand with scales of mica .... Earth separated by the sieve 100 parts of the earth contain : Silica x - ... Alumina - - - - Peroxide and protoxide of iron - Peroxide of manganese Carbonate of lime - - Carbonate of magnesia Potash combined with silica Soda, principally combined with silica Phosphoric acid combined with lime - Sulphuric acid, idem Chlorine in common salt Humus soluble in alkalies Humus with nitrogenous organic matter 2-408 97-592 100-000 59-581 . 3-224 4-896 0-720 17-953 11-075 0-150 0-891 0-846 - 0-004 0-004 0-536 0-120 100-000 BELGIUM. 38. Surface-soil of a field distinguished for its fertility. It had received no manure for twelve years previous to the time at which the analysis was executed. The ro- tation of crops for the latter nine years was as follows : 1. beans, 2. barley, 3. potatoes, 4. winter barley with red clover, 5. clover, 6. winter barley, 7. wheat, 8. oats ; during the ninth year 'it was allowed to lie fallow. The soil is more clayey than loamy, and of a very fine grain. Water extracted from the soil, 0-013 soda, 0-002 lime, 0-012 mag- nesia, 0-009 sulphuric acid, 0-003 potash, 0-003 chlorine, with traces of silica and hu- mus. 100 parts contained : Silica - .... Alumina .... Peroxide and protoxide of iron Peroxide of manganese Carbonate of lime Carbonate of magnesia Potash, principally combined with silica Soda ..... Phosphoric acid ... Sulphuric acid Chlorine - ... Humus - . 64-517 4-810 - 8-316 0-800 . 9-403 10-361 o-ioo . 0-013 1-221 0-009 0-003 - 0-447 100-000 ENGLAND. 39. Surface-soil of a very fertile sandy field from the vicinity of Tunbridge, Kent, according to Davy. 100 parts consisted of: Loose stones and gravel Sand and silica - Alumina ... Peroxide of iron Carbonate of lime Carbonate of magnesia Common salt and extractive matter Gypsum Matter destructible by heat Vegetable fibre .... Water Loss - - - 13-250 58-250 3-250 1-250 4.750 0.750 0-750 0-500 3-750 3-500 5-000 5-000 100-000 The great Davy, who was convinced of the importance of the inorganic constituents of soils, has omitted to detect the phospho- ric acid, potash, soda, and manganese. All of these must have been present in the soil, for we are informed that it produced good hops, for which these ingredients are indis- pensable. 40. A good turnip soil from Holkham, Norfolk, yielded to Davy : Siliceous sand .... 88.888 Silica - - - - 1-666 Alumina .... 1-222 Peroxide of iron, ... 0'334 Carbonate of lime ... 7-QOO Vegetable and saline matter - - 0'556 Moisture .... Q-334 100-000 In this case also, phosphoric acid, man- ganese, potash, magnesia, &c., have es- caped detection by this acute chemist ; yet doubtless they must be present in the soil, for we are informed that it produces good turnips. 41. An excellent wheat soil from the neighbourhood of West Drayton, Middle- sex, according to Davy. 100 parts con- tained : Sand and silica .... 72-800 Alumina .... 11*600 Carbonate of lime - 11-200 Humus and moisture ... 4.400 100-000 This analysis has been executed so imper- fectly, that it only conveys a very feeble representation of the nature of the soil. A soil which bears good wheat must contain phosphate of potash, soda, chlorine, and sulphuric acid ; yet none of these are exhi- bited by the analysis. 42. Sui face-soil of a fertile field in the neighbourhood of Bristol. 100 parts con- tained : Silica and siliceous sand Alumina .... Peroxide of iron .... Lime (carbonate) ... Magnesia .... Humus ... Saline and extractive matter Water 100-000 Davy has made several analyses of vari- ous fertile soils, and since his time numerous other analyses have been published; but they are all so superficial, and in most cases so inaccurate, that we possess no means of ascertaining the composition or nature of English arable land. SWEDEN. 43. Surface-soil of a field which produces the most abundant crops, and has never been manured. (Berzelius.) 100 parts con- sist of: Siliceous sand .... 57*900 Silica 14-500 Alumina ..... 2'000 Phosphates of lime and iron - - 6'000 Carbonate of lime 11.100 , Carbonate of magnesia 1.000 I Insoluble extractive matter - - T250 CONSTITUENTS OF SOILS. 83 Insoluble extractive matter destructible by heat - 4-000 Animal matter ..... 1-600 Resin - ... 0'250 Loss - ... Q'400 lOO'OOO This great chemist has strangely omitted to detect in the soil potash, soda, chlorine, sulphuric acid, and manganese. As this soil is eminent for its fertility, there cannot be the slightest doubt that all these ingre- dients must have existed in it in notable quantity. ISLAND OF JAVA. 44. A very fine-grained loamy soil, co- loured yellow by peroxide of iron, consisted of: Silica and siliceous sand Alumina ... Peroxide and protoxide of iron Peroxide of manganese Lime .... Magnesia Potash, principally in combination silica .... Soda, idem ... Phosphoric acid - Sulphuric acid Chlorine ... Humus ... Water with carbonic acid with 67-660 13-572 10-560 1-640 0-912 0-570 0-030 0184 0-391 0.038 o-oio 0-368 4-065 100-000 WEST INDIES (PORTO RICO.) 45. Surface-soil of a very barren field. 100 parts contained : Silica and siliceous sand - - - 70'900 Alumina .... 6'996 Peroxide and protoxide of iron (much mag- netic iron sand) ... 6"102 Peroxide of manganese - - 0'200 Lime 2-218 Magnesia .... 3*280 Potash 0-130 Carbonate of soda . - . 6'556 Phosphoric acid, combined with lime - 1-362 Sulphuric acid, combined with lime 0'149 Chlorine in common salt 0*067 Humus, soluble in alkalies - - 0'540 Humus ..... 1-500 100-000 This soil is improved by gypsum. Its sterility is due to the excessive quantity of carbonate of soda which is present. NORTH AMERICA. 46. Surface-soil of alluvial land in Ohio, remarkable for its great fertility. 100 parts consisted of: Silica and fine siliceous sand - - 79'538 Alumina, - 7'306 Peroxide and protoxide of iron, (much magnetic iron sand) - - 5'824 Peroxide of manganese - - T320 Lime ..... 0619 Magnesia ... 1-024 Potash, principally combined with silica 0'200 Soda - - - 0'024 Phosphoric acid combined with lime and oxide of iron - - - 1'776 Sulphuric acid, combined with lime - - 122 Chlorine - - - 0036 Humus, soluble in alkalies Nitrogenous organic matter Wax and resinous matter 100-000 47. (A.) Surface-soil of a mountainous district in the neighbourhood of Ohio. (B.) Analysis of the subsoil. This soil is also distinguished for its great fertility. 100 parts contain : .(A) - 87-143 - 5-666 - 2-220 - 0-360 - 0-564 0-312 (B) 94261 1-376 2-336 1-200 0.243 0-310 0-240 a trace 0-034 a trace Silica, with fine siliceous sand Alumina .... Peroxide and protoxide of iron Peroxide of manganese - Lime ... Magnesia Potash, principally combined with silica 0'120 Soda - - - - 0'025 Phosphoric acid - - 0'060 Sulphuric acid - - - 027 Chlorine .... 0'036 Humus soluble in alkalies 1.304 Humus .... T072 Carbonic acid, combined with lime O'OSO Nitrogenous organic matter - 1*011 100-000 100-000 In the preceding part of the chapter we have inserted a number of analyses of vari- ous soils, as well as the conclusions deduced from them, by means of which the farmer may be enabled to ascertain the manures best adapted for each variety of soil. By in- specting the analyses of the sterile soils, it will be apparent that it is in the power of chemistry to point out the causes of their sterility. The general cause which con- duces to the sterility of soils is either the ab- sence of certain constituents indispensable for the growth of plants, or the presence of others which exert an injurious or poi- sonous action. The analyses are those of Dr. Sprengel, a chemist who has unceas- ingly occupied himself for the last twenty years in endeavouring to point out the im- portance of the inorganic ingredients of a soil for the developement of plants cultivated upon it. He considers as essential all the inorganic bodies found in the ashes of plants. Now, although we cannot coincide with him, in the opinion that iron and manganese are indispensable for vegetable life, (for these bodies are found as excrementitious matter only in the bark, and never form a constitu- ent of an organ,) yet we gratefully acknow- ledge the valuable services which he has ren- dered to agriculture, by furnishing a natural explanation of the action of ashes, marl, &c., in the improvement of a soil. Sprengel has shown that these mineral manures af- ford to a soil alkalies, phosphates, and sul- phates ; and further, that they can exert a notable influence only on those soils in which they are absent or deficient. In a former chapter of this book I have endea- voured to point out the importance of consi- dering these constituents as intimately con- nected with the vital processes of the vege- table organism, and have shown that the different families of plants contain unequal 84 AGRICULTURAL CHEMISTRY. quantities of inorganic ingredients. This subject has been left unexamined by Spren- gel, yet it is one of much importance ; for the application of manures must be regulated by the composition of the plants which are cultivated on any particular soil. Still the composition of the soil must always be kept in view. Thus it would be perfect extrava- gance to manure certain soils with marl, ashes, or gypsum ; whilst, on the contrary, these compounds would produce the most beneficial results on other lands. In a former part of the work, the princi- pal action of gypsum upon vegetation was ascribed to the decomposition and fixation of the carbonate of ammonia contained in rain-water; but gypsum exerts a twofold action. The power of decomposing car- bonate of ammonia, and of fixing the am- monia, is not peculiar to gypsum, but is shared also by other salts of lime (chloride of calcium, for example.) But it acts also as a sulpluite, and when useful as such can- not be replaced by any other salt of lime which does not contain sulphuric acid. Hence gypsum can be replaced as a ma- nure only by a mixture of a salt of lime with ammonia, and a salt of sulphuric acid. Sulphate of ammonia can therefore be sub- stituted for gypsum, and exerts a more rapid and effectual action. In France, sulphuric acid has been poured upon the fields after the removal of the crops, and has been found to form a good manure. But this is merely a process for forming gypsum in situ ; for the soils upon which it is applied contain much lime, which enters into com- bination with the sulphuric acid. It would certainly be much more advantageous to form sulphate of ammonia by adding the acid to putrefied urine, and to apply this mixture to the field. APPENDIX TO PART I. EXPERIMENTS AND OBSERVATIONS ON THE ACTION OF CHARCOAL FROM WOOD ON VEGETATION. BY EDWARD LUKAS.* " IN a division of a low hot-house in the botanical garden at Munich, a bed was set apart for young tropical plants, but instead of being filled with tan, as is usually the case, it was filled with the powder of char- coal, (a material which could be easily pro- cured,) the large pieces of charcoal having been previously separated by means of a sieve. The heat was conducted by means of a tube of white iron into a hollow space in this bed, and distributed a gentle warmth, such as tan communicates, when in a state of fermentation. The plants placed in this bed of charcoal quickly vegetated, and ac- quired a healthy appearance. Now, as is always the case in such beds, the roots of many of the plants penetrated through the holes in the bottom of the pots, and then spread themselves out; but these plants evidently surpassed in vigour and general luxuriance plants grown in the common way for example, in tan. Seve- ral of them, of which I shall only specify the beautiful TJmnbergia data, and the ge- nus Peireskice, throve quite astonishingly; the blossoms of the former were so rich, that all who saw it affirmed they had never before seen such a specimen. It produced alo a number of seeds without any artificial aid, while in most cases it is necessary to apply the pollen by the hand. The Peires- kice grew so vigorously, that the P. aculeata produced shoots several ells in length, and the P. grandifolia acquired leaves a foot in length. These facts, as well as the quick germina- tion of the seeds which had been scattered spontaneously, and the abundant appearance of young Filices, naturally attracted my at- tention, and I was gradually led to a series * See page 27. of experiments, the results of which may not be uninteresting ; for, besides being of practical use in the cultivation of most plants, they demonstrate also several facts of importance to physiology. The first ex- periment which naturally suggested itself was to mix a certain proportion of charcoal with the earth in which different plants grew, and to increase its quantity according as the advantage of the method was per- ceived. An addition of f charcoal, for exam- ple, to vegetable mould, appeared to answer excellently for the Gesnena and Gloxinia, and also for the tropical Jlroidecz with tube- rous roots. The first two soon excited the attention of connoisseurs, by the great beauty of all their parts and their general appearance. They surpassed very quickly those cultivated in the common way, both in the thickness of their stems and dark colour of their leaves ; their blossoms were beautiful, and their vegetation lasted much longer than usual, so much so, that in the middle of November, when other plants of the same kinds were dead, these were quite fresh and partly in bloom. Jlroidecz took root very rapidly, and their leaves surpassed much in size the leaves of those not so treated; the species which are reared as ornamental plants on account of the beauti Iful colouring of their leaves, (I mean such [as the Caladium bicolor, Pictum, Pcecile, \ Sec.,) were particularly remarked for the I liveliness of their tints ; and it happened here also, that the period of their vegetation was unusually long. A cactus planted in a mixture of equal parts of charcoal and earth throve progressively, and attained double of 1 its former size in the space of a few weeks. I The use of the charcoal was very advan- APPENDIX TO PART I. 85 tageous with several of the Bromeliacece, and LalacecK, with the Citrus, and Begonia also, and even with the Palmce. The same ad- vantage was found in the case of almost all those plants for which sand is used, in order to keep the earth porous, when charcoal was mixed with the soil instead of sand; the vegetation was always rendered stronger and more vigorous. " At the same time that these experiments were performed with mixtures of charcoal with different soils, the charcoal was also used free from any addition, and in this case the best results were obtained. Cuts of plants from different genera took root in it well and quickly; I mention here only the Euphorbia fastuosa and ftdgens which took root in ten days, Pandanus utilis in three months, P. amaryllifolius, Chamcedorea ela- tior in four weeks, Piper nigrum, Begonia, Ficus, Cecropia, Chiococca, Buddleya, Hakea, Phyllanthus, Capparis, Laurus, Stifftia, Jac- auinia, Mimosa, Cactus, in from eight to ten days, and several others amounting to forty species, including Ilex, and many others. Leaves, and pieces of leaves, and even pe- duncidi, or petioles, took root and in part budded in pure charcoal. Amongst others we may mention the foliola of several of the Cycadece as having taken root, as also did parts of the leaves of the Begonia Tclfairice, and Jacaranda brasiliensis ; leaves of the Euphorbia fastuosa, Oxalis Barrilieri, Ficus, Cyclamen, Polyanthcs, Mesembryanthemum ; also the delicate leaves of the Lophospermum and Martynia, pieces of a leaf of the Jlgave umericana; tufts of Pinus, &,c.; and all with- out the aid of a previously formed bud. " Pure charcoal acts excellently as a means of curing unhealthy plants. A Do- riantlies excelsa, for example, which had been drooping for three years, was rendered completely healthy in a very short time by this means. An orange-tree which had the very common disease in which the leaves become yellow, acquired within four weeks its healthy green colour, when the upper surface of the earth was removed from the pot in which it was contained, and a ring of charcoal of an inch in thickness strewed in its place around the periphery of the pot. The same was the case with trie Gardenia. " I should he led too far were I to state all the results of the experiments which I have made with charcoal. The object of this paper is merely to show the general effect exercised by this substance on vegetation ; but the reader who takes particular interest in the subject will find more extensive ob- servations in the 'Jlllgemeinc Deutsche Garten- zeitung* of Otto ancl Dietrich, in Berlin; or Loudon's Gardener's Magazine for March, 1841. " The charcoal employed in these experi- ments was the dust-like powder of charcoal from firs and pines, such as is used in the forges of blacksmiths, and may be easily procured in any quantity. It was found to have most effect when allowed to lie during the winter exposed to the action of the air. In order to ascertain the effects of different kinds of charcoal^ experiments were also made upon that obtained from the hard woods and peat, and also upon animal char- coal, although I foresaw the probability that none of them would answer so well as that of pine-wood, both on account of its porosity and the ease with which it is decomposed.* " It is superfluous to remark, that in treat- ing plants in the manner here described, they must be plentifully supplied with water, since the air having such free access pene- trates and dries the roots, so that unless this precaution is taken, the failure of all such experiments is unavoidable. " The action of charcoal consists primarily in its preserving the parts of the plants with which it is in contact whether they be 1 roots, branches, leaves, or pieces of leaves unchanged in their vital power for a long 1 space of time, so that the plant obtains time to develope the organs which are necessary for its further support and propagation. There can scarcely be a doubt also that the charcoal undergoes decomposition ; for after being used five to six years it becomes a coaly earth; and if this is the case, it must yield carbon, or carbonic oxide, abundantly to the plants growing in it, and thus afford the principal substance necessary for the nutrition of vegetables. f In what other manner indeed can we explain the deep green colour and great .luxuriance of the leaves and every part of the plants, which can be obtained in no other kind of soil, ac- cording to the opinion of men well qualified to judge? It exercises likewise a favourable influence by decomposing and absorbing the matters excreted by the roots, so as to keep the soil free from the putrefying substances which are often the cause of the death of the spongiolce. Its porositv, as well as the power which it possesses of absorbing water with rapidity, and, after its saturation, of allow- ing all other water to sink through it, are causes also of its favourable effects. These experiments show what a close affinity the component parts of charcoal have to all plants, for every experiment was crowned with success, although plants belonging to a * M. Lukas has recently repeated these experi- ments, and found that the animal charcoal ob- tained by the calcination of bones possesses a de- cided advantage over all other kinds of charcoal, which he subjected to experiment. Liebig's An- nalen, Sand xxxix. Heft I. S. 127. t As some misconception has arisen regarding this explanation of the action of charcoal upon ve- getation, and an idea propagated that the intro- duction of these opinions into this work incorpo- rated them with those of Liebig, it is necessary to state that they are merely inserted here as part of the papers of M. Lukas. The true explanation has been given in a former part of the work, viz., that charcoal possesses the power of absorbing; carbonic acid and ammonia from the atmosphere, which serve fo: the nourishment of plants. ED. AGRICULTURAL CHEMISTRY. great many different families were subjected to trial." (Biichner's Repertoriwn, ii. ReUie, xix. Bd. S. 38.) ON A MODE OF MANURING VINES. The observations contained in the follow- ing pages should be extensively known, be- cause they furnish a remarkable proof of the principles which have been stated in the preceding part of the work, both as to the manner in which manure acts, and on the origin of the carbon and nitrogen of plants. They prove that a vineyard may be re- tained in fertility without the application of animal matters, when the leaves and branches pruned from the vines are cut into small pieces and used as manure. According to the first of the following statements, both of which merit complete confidence, the perfect fruitful ness of a vineyard has been maintained in this manner for eight yeans, and according to the second statement for ten years. Now, during this long period, no carbon v/as conveyed to the soil, for that contained in the pruned branches was the produce of the plant itself, so that the vines were placed exactly in the same condition as trees in a forest which received no manure. Under ordinary circumstances a manure containing potash must be used, otherwise the fertility of the soil will decrease. This is done in all wine-countries, so that alkalies to a very considerable amount must be extracted from the soil. When, however, the method of manuring now to be described is adopted, the quantity of alkalies exported in the wine does not exceed that which the progressive disinte- gration of the soil every year renders capable of being absorbed by the plants. On the Rhine 1 litre of wine is calculated as the yearly produce of a square metre of land (10-8 square feet English.) Now if we suppose that the wine is three-fourths satu- rated with cream of tartar, a proportion much above the truth, then we remove from every square metre of land with the wine only 1-8 gramme of potash. 1000 grammes (1 litre) of champagne yield only 1.54, and the same quantity of Wachenheimer 1-72 of a residue which after being heated to red- ness is found to consist of carbonates. One vine-stock, on an average, grows on every square metre of land, and 1000 parts of the pruned branches contain 56 to 60 parts of carbonate, or 38 to 40 parts of pure potash. Hence it is evident that 45 grammes, or 1 ounce, of these branches contain as much potash as 1000 grammes (1 litre) of wine. But from ten to twenty times this quantity of branches are yearly taken from the above extent of surface. In the vicinity of Johannisberg, Rudes- heim, and Budesheim, new vines are not planted after the rooting out of the old stocks, until the land has lain for five or six years in barley and esparcet, or lucerne; in the sixth year the young slocks are planted, but not manured till the ninth. ON THE MANURING OF THE SOIL IN VINE- YARDS.* " In reference to an article in your paper, No. 7, 1838, and No. 29, 1839, I cannot omit the opportunity of again calling the public attention to the fact, that nothing more is necessary for the manure of a vine- yard than the branches which are cut from the vines themselves. " My vineyard has been manured in this way for eight years, without receiving any other kind of manure, and yet more beauti- ful and richly laden vines could scarcely be pointed out. I formerly followed the method usually practised in this district, and was obliged in consequence to purchase manure to a large amount. This is now entirely saved, and my land is in excellent condition. " When I see the fatiguing labour used in the manuring of vineyards horses and men toiling up the mountains with unne- cessary materials I feel inclined to say to all, Come to my vineyard and see how a bountiful Creator has provided that vines shall manure themselves, like the trees in a forest, and even better than they! The foliage falls from trees in a forest, only when they are withered, and they lie for years before they decay; but the branches are pruned from the vine in the end of July or beginning of August whilst still fresh and moist. If they are then cut into small pieces and mixed with the earth, they undergo putrefaction so completely, that, as I have learned by experience, at the end of four weeks not the smallest trace of them can be found." " REMARKS OF THE EDITOR. We find the following notices of the same fact in Henderson's ' Geschichte der Weine der alien und neuen Zeit:' " * The best manure for vines is the branches pruned from the vines themselves, cut into small pieces, and immediately mixed with the soil.' "These branches were used as manure long since in the Bergstrasse. M. Frauen- felder says:f " ' I remember that twenty years ago, a man called Peter Muller had a vineyard here which he manured with the branches pruned from the vines, and continued this practice for thirty years. His way of apply- ing them was to hoe them into the soil after having cut them into small pieces. " ' His vineyard was always in a thriving * Slightly abridged from an article by M. Kreba of Seeheim, in the " Zeitschrift fur die landwinh- schaftlichen Vereine des Grosherzogthums Hes- sen." No. 28, July 9, 1840. t Badisches landwirthschaftliches "YVochenblatt, v. 1834, S. 52 and 79. CHEMICAL TRANSFORMATIONS. 87 condition; so much so indeed, that the pea- sants here speak of it to this day, wondering that old Miiller had so good a vineyard, and yet used no manure.' " Lastly, Wilhelrn Ruf of Schriesheim writes: ' * For the last ten years I have been unable to place dung on my vineyard, be- cause I am poor and can buy none. But I was very unwilling to allow my vines to decay, as they are my only source of sup- port in my old age; and I often walked very anxiously amongst them, without knowing what I should do. At last my necessities became greater, which made rne more at- tentive, so that I remarked that the grass was longer on some spots where the branches of the vine fell than on those on which there were none. So I thought upon the matter, and then said to myself: If these branches can make the grass large, strong, and green, they must also be able to make my plants grow better, and become strong and green. I dug therefore my vineyard as deep as if I would put dung into it, and cut the branches j into pieces, placing them in the holes and j covering them with earth. In a year I had the very great satisfaction to see my barren vineyard become quite beautiful. This plan I continued every year, and now my vines grow splendidly, and remain the whole summer green, even in the greatest heat. " * All my neighbours wonder very much how my vineyard is so rich, and that I ob- tain so many grapes from it, and yet they all know that I have put no dung upon it for ten years.' " PART II. OF THE CHEMICAL PROCESSES OF FERMENTATION, DECAY AND PUTRE- FACTION. CHAPTER 1. CHEMICAL, TRANSFORMATIONS. WOODY fibre, sugar, gum, and all such organic compounds, suffer certain changes when in contact with other bodies, that is, they suffer decomposition. There are two distinct modes in which these decompositions take place in organic chemistry. When a substance composed of two com- pound bodies, crystallized oxalic acid for example, is brought in contact with concen- trated sulphuric acid, a complete decompo- sition is effected upon the application of a gentle heat. Now crystallized oxalic acid is a combination of water with the anhy- drous acid; but concentrated sulphuric acid possesses a much greater affinity for water than oxalic acid, so that it attracts all the water of crystallization from that substance. In consequence of this abstraction of the water, anhydrous oxalic acid is set free ; but as this acid cannot exist in a free state, a division of its constituents necessarily en- sues, by which carbonic acid and carbonic oxide are produced, and evolved in the gaseous form in equal volumes. In this example, the decomposition is the conse- quence of the removal of two constituents (the elements of water,) which unite with the sulphuric acid, and its cause is the supe- rior affinity of the acting body (the sulphuric acid) for water. In consequence of the re- moval of the component parts of water, the remaining elements enter into a new form ; in place of oxalic acid, we have its elements in the form of carbonic acid and carbonic oxide. This form of decomposition, in which the change is effected by the agency of a body which unites with one or more of the con- stituents of a compound, is quite analogous to the decomposition of inorganic substances. When we bring sulphuric acid and nitrate of potash together, nitric acid is separated in consequence of the affinity of sulphuric acid for potash ; in consequence, therefore, of the formation of a new compound (sul- phate of potash.) In the second form of these decomposi- tions, the chemical affinity of the acting body causes the component parts of the body which is decomposed to combine so as to form new compounds, of which either both, or only one, combine with the acting body. Let us take dry wood, for example, and moisten it with sulphuric acid ; after a short time the wood is carbonised, while the sulphuric acid remains unchanged, with the exception of its being united with more water than it possessed before. Now this water did not exist as such in the wood, although its elements, oxygen and hydro- gen, were present ; but by the chemical at- traction of sulphuric acid for water, they were in a certain measure compelled to unite in this form; and in consequence of this, the carbon of wood was separated as charcoal. Hydrocyanic acid, and water, in contact with hydrochloric acid, are mutually decom- posed. The nitrogen of the hydrocyanic acid, and a certain quantity of the hydrogen of the water, unite together and form am- monia; whilst the carbon and hydrogen of the hydrocyanic acid combine with the oxy- gen of the water, and form formic acid. The ammonia combines with the muriatic acid. Here the contact of muriatic acid with water and hydrocyanic acid causes a disturbance in the attraction of the elements of both compounds, in consequence of which they arrange themselves into new combinations. 88 AGRICULTURAL CHEMISTRY. one of which ammonia possesses the power of uniting with the acting body. Inorganic chemistry can present instances analogous to this class of decomposition also ; but there are forms of organic chemi- cal decomposition of a very different kind, in which none of the component parts of the matter which suffers decomposition enter into combination with the body which de- termines the decomposition. In cases of this kind a disturbance is produced in the mutual attraction of the elements of a com- pound, and they in consequence arrange themselves into one or several new combi- nations, which are incapable of suffering further change under the same conditions. When, by means of the chemical affinity of a second body, by the influence of heat, or through any other causes, the composi- tion of an organic compound is made to undergo such a change, that its elements form two or more new compounds, this manner of decomposition is called a chemi- cal transformation or metamorphosis. It is an essential character of chemical transfor- mations, that none of the elements of the body decomposed are singly set at liberty. The changes, which are designated by the terms fermentation, decay, and putrefaction, are chemical transformations effected by an agency which has hitherto escaped atten- tion, but the existence of which will be proved in the following pages. CHAPTER II ON THE CAUSES WHICH EFFECT FERMENTA- TION,, DECAY,* AND PUTREFACTION. ATTENTION has been recently directed to the fact, that a body in the act of combina- tion or decomposition exercises an influence upon any other body with which it may be in contact. Platinum, for example, does not decompose nitric acid ; it may be boiled with this acid without being oxidized by it, even when in a state of such fine division, that it no longer reflects light (black spongy platinum.) But an alloy of silver and pla- tinum dissolves with great ease in nitric acid ; the oxidation whi-ch the silver suffers causes the platinum to submit to the same change ; or, in other words, the latter body from its contact with the oxidizing silver acquires the property of decomposing nitric acid. Copper does not decompose water, even when boiled in dilute sulphuric acid; bu * An essential distinction is drawn in the follow ing part of the work, between decay and putre faction (Verwesung und Faulniss,) and they are shown to depend on different causes ; but as the word decay is not generally applied to a distinc species of decomposition, and does not indicate it true nature, I shall in future, at the suggestion o he author, employ the term eremacausis, th meaning of which has been already explained. ED n alloy of copper, zinc, and nickel, dis- olves easily in this acid with evolution of lydrogen gas. Tin decomposes nitric acid with great fa- ility, but water with difficulty ; and yet, yhen tin is dissolved in nitric acid, hydrogen s evolved at the same time, from a decom- wsition of the water contained in the acid, nd ammonia is formed in addition to oxide f tin. In the examples here given the only com- ination or decomposition which can be ex- ilained by chemical affinity is the last. In he other cases, electrical action ought to lave retarded or prevented the oxidation of he platinum or copper while they were in :ontact with silver or zinc, but, as experience hows, the influence of the opposite electri- :al conditions is more than counterbalanced >y chemical actions. The same phenomena are seen in a less dubious form in compounds, the elements f which are held together only by a feeble ffinity. It is well known that there are chemical compounds of so unstable a nature, hat changes in temperature and electrical :ondition, or even simple mechanical fric- tion, or contact with bodies of apparently .otally indifferent natures, cause such a dis- turbance in the attraction of theiramstituents, that the latter enter into new forms, with- out any of them combining with the acting body. These compounds appear to stand but just within the limits of chemical combi- nation, and agents exercise a powerful influ- ence over them, which are completely de- void of action on compounds of a stronger affinity. Thus, by a slight increase of tem- perature, the elements of hypochlorous acid separate from one another with evolution of heat and light; chloride of nitrogen explodes by contact with many bodies, which com- bine neither with chlorine nor nitrogen at common temperatures ; and the contact of any solid substance is sufficient to cause the explosion of iodide of nitrogen, or fulminat- ing silver. It has never been supposed that the causes of the decomposition of these bodies should be ascribed to a peculiar power, different from that which regulates chemical affinity, a power which mere contact with the down of a feather is sufficient to set in activity, and which, once in action, gives rise to the decomposition. These substances have always been viewed as chemical compounds of a very unstable nature, m which the component parts are in a state of such ten- sion, that the least disturbance overcomes their chemical affinity. They exist only by the vis inertias, and any shock or movement is sufficient to destroy the attraction of their component parts, and consequently their existence in their definite form. Peroxide of hydrogen belongs to this class of bodies ; it is decomposed by all substances capable of attracting oxygen from it, and even by contact with many bodies, such as platinum or silver, which do not enter into CHEMICAL TRANSFORMATIONS. 89 combination with any of its constituents. In this respect, its decomposition depends evidently upon the same causes which effect that of iodide of nitrogen, or fulminating silver. Yet it is singular that the cause of the sudden separation of the component parts of peroxide of hydrogen has been viewed as different from those of common de- composition, and has been ascribed to a new power termed the catalytic force. Now, it has not been considered, that the presence of the platinum and silver serves here only to accelerate the decomposition ; for without the contact of these metals, the peroxide of hydrogen decomposes spontaneously, al- though very slowly. The sudden separa- tion of the constituents of peroxide of hydro- gen differs from the decomposition of gase- ous hypochlorous acid, or solid iodide of nitrogen, only in so far as the decomposition takes place in a liquid. A remarkable action of peroxide of hydro- gen has attracted much attention, because it differs from ordinary chemical phenomena. This is the reduction which certain oxides suffer by contact with this substance, on the instant at which the oxygen separates from the water. The oxides thus easily reduced, are those of which the whole, or part at least, of their oxygen is retained merely by a feeble affinity, such as the oxides of silver and of gold, and peroxide of lead. Now, other oxides which are very stable in composition, effect the decomposition of peroxide of hydrogen, without experiencing the smallest change; but when oxide of silver is employed to effect the decomposi- tion, all the oxygen of silver is carried away with that evolved from the peroxide of hy- drogen, and as a result of the decomposition, water and metallic silver remain. When peroxide of lead is used for the same pur- pose, half its oxygen escapes as a gas. Per- oxide of manganese may in the same man- ner be reduced to the protoxide, and oxygen set at liberty, if an acid is at the same time present, which will exercise an affinity for the protoxide and convert it into a soluble salt. If, for example, we add to peroxide of hydrogen sulphuric acid, and then per- oxide of manganese in the state of fine pow- der, much more oxygen is evolved than the compound of oxygen and hydrogen could yield ; and if we examine the solution which remains, we find a salt of the protoxide of manganese, so that half of the oxygen has been evolved from the peroxide of that metal. A similar phenomenon occurs, when car- bonate of silver is treated with several or- ganic acids. Pyruvic acid, for example, combines readily with pure oxide of silver, and forms a salt of sparing solubility in water. But when this acid is brought in contact with carbonate of silver, the oxygen of part of the oxide escapes with the car- bonic acid, and metallic silver remains in the state of a black powder. (Berzelius.) Now no other explanation of these phe- nomena can be given, than that a body in 12 the act of combination or decomposition enables another body, with which it is in contact, to enter into the same state. It is evident that the active state of the atoms of one body has an influence upon the atoms of a body in contact with it; and if these atoms are capable of the same change as the former, they likewise undergo that change ; and combinations and decompositions are the consequence. But when the atoms of the second body are not capable of such an action, any further disposition to change ceases from the moment at which the atoms of the first body assume the state of rest, that is when the changes or transformations of this body are quite completed. This influence exerted by one compound upon the other, is exactly similar to that which a body in the act of combustion exer- cises upon a combustible body in its vicinity ; with this difference only, that the causes which determine the participation and du- ration of these conditions are different. For the cause, in the case of the combustible body, is heat, which is generated every mo- ment anew; whilst in the phenomena of decomposition and combination, which we are considering at present, the cause is a body in the state of chemical action, which exerts the decomposing influence only so long as this action continues. Numerous facts show that motion alone exercises a considerable influence on chemi- j cal forces. Thus, the power of cohesion ! does not act in many saline solutions, even when they are fully saturated with salts, if they are permitted to cool while at rest. In such a case, the salt dissolved in a liquid ! does not crystallize; but when a grain of ! sand is thrown into the solution, or when it receives the slightest movement, the whole I liquid becomes suddenly solid while heat is evolved. The same phenomenon happens with water, for this liquid may be cooled much under 32 F. (0 C.,) if kept com- pletely undisturbed, but solidifies in a mo- ment when put in motion. The atoms of a body must in fact be set in motion before they can overcome the vis inertice so as to arrange themselves into cer- tain forms. A dilute solution of a salt of potash mixed with tartaric acid yields no precipitate whilst at rest; out if motion is communicated to the solution by agitating it briskly, solid crystals of cream of tartar are deposited. A solution of a salt of mag- nesia also, which is not rendered turbid by the addition of phosphate of ammonia, de- posits the phosphate of magnesia and am- monia on those parts of the vessel touched with the rod employed in stirring. In the processes of combination and de- composition under consideration, motion, by overcoming the vis inerlice, gives rise im- mediately to another arrangement of the atoms of a body, that is, to the production of a compound which did not before exist in it. Of course these atoms must previously possess the power of arranging themselves H2 90 CHEMICAL TRANSFORMATIONS. in a certain order, otherwise both friction and motion would be without the smallest influence. The simple permanence in position of the atoms of a body, is the reason that so many compounds appear to present themselves, in conditions, and with properties, different from those which they possess, when they obey the natural attractions of their atoms. Thus sugar and glass, when melted and cooled rapidly, are transparent, of a con- choidai fracture., and elastic and flexible to a certain degree. But the former becomes dull and opaque on keeping, and exhibits crystalline faces by cleavage, which belong to crystallized sugar. Glass assumes also the same condition, when kept soft by heat for a long period ; it becomes white, opaque, and so hard as to strike fire with steel. Now, in both these bodies, the compound molecules evidently have different positions in the two forms. In the first form their at- traction did not act in the direction in which their power of cohesion was strongest. It is known also, that when sulphur is melted and cooled rapidly by throwing it into cold water, it remains transparent, elastic, and so soft that it may be drawn out into long threads ; but that after a few hours or days, it becomes again hard and crystalline. The remarkable fact here is, that the amorphous sugar or sulphur returns again into the crystalline condition, without any assistance from an exterior cause; a fact which shows that their molecules have as- sumed another position, and that they pos- sess, therefore, a certain degree of mobility, even in the condition of a solid. A very rapid transposition or transformation of this kind is seen in arragonite, a mineral which possesses exactly the same composition as calcareous spar, but of which the hardness and crystalline form prove that its molecules are arranged in a different manner. When a crystal of arragonite is heated, an interior motion of its molecules is caused by the ex- pansion ; the permanence of their arrange- ment is destroyed ; and the crystal splinters with much violence, and falls into a heap of small crystals of calcareous spar. It is impossible for us to be deceived re- garding the causes of these changes. They are owing to a disturbance of the state of the equilibrium, in consequence of which the particles of the body put in motion obey other affinities or their own natural attrac- tions. But if it is true, as we have just shown it to be, that mechanical motion is sufficient to cause a change of condition in many bodies, it cannot be doubted that a body in the act of combination or decomposition is capable of imparting the same condition of motion or activity in which its atoms are to certain other bodies : or in other words, to enable other bodies with which it is in con- tact to enter into combinations, or suffer de- compositions. The reality of this influence has been al- ready sufficiently proved by the facts de- rived from inorganic chemistry, but it is of much more frequent occurrence in the re- lations of organic matter, and causes very striking and wonderful phenomena. By the terms fermentation, jmtrefaction, and eremacausis, are meant tnose changes in form and properties which compound or- ganic substances undergo when separated from the organism, and exposed to the in- fluence of water and a certain temperature. Fermentation and putrefaction are examples of that kind of decomposition, which we have named transformations : the elements of the bodies capable of undergoing these changes arrange themselves into new com- binations, in which the constituents of water generally take a part. Eremacausis (or decay) differs from fer- mentation and putrefaction, inasmuch as it cannot take place without the access of air, the oxygen of which is absorbed by the de- caying bodies. Hence it is a process of slow combustion, in which heat is uni- formly evolved, and occasionally even light. In the processes of decomposition termed fermentation and putrefaction, gaseous pro- ducts are very frequently formed, which are either inodorous, or possess a very offensive smell. The transformations of those matters which evolve gaseous products without odour are now, by pretty general consent, designated by the term fermentation ; whilst to the spontaneous decomposition of bodies which emit gases of a disagreeable smell, the term putrefaction is applied. But the smell is of course no distinctive character of the nature of the decomposition, for both fermentation and putrefaction are processes of decomposition of a similar kind, the one of substances destitute of nitrogen, the other of substances which contain it. It has also been customary to distinguish from fermentation and putrefaction a par- ticular class of transformations, viz., those in which conversions and transpositions are effected without the evolution of gaseous products. But the conditions under which the products of the decomposition present themselves are purely accidental; there is therefore no reason for the distinction just mentioned. CHAPTER III. FERMENTATION AND PUTREFACTION. SEVERAL bodies appear to enter sponta- neously into the states of fermentation and putrefaction, particularly such as contain nitrogen or azotised substances. Now, it is very remarkable, that very smoll quantities of these substances, in a state of fermenta- tion or putrefaction, possess the power of causing unlimited quantities of similar mat- ters to pass into the same state. Thus, a CHEMICAL TRANSFORMATIONS. 91 small quantity of the juice of grapes in the act of fermentation, added to a large quan- tity of the same fluid, which does not fer- ment, induces the state of fermentation in the whole mass. So likewise the most mi- nute portion of milk, paste, juice of the beet-root, flesh, or blood, in the state of putrefaction, causes fresh milk, paste, juice of the beet-root, flesh or blood, to pass into the same condition when in contact with them. These changes evidently differ from the class of common decompositions which are effected by chemical affinity; they are chemical actions, conversions, or decompo- sitions, excited by contact with bodies al- ready in the same condition. In order to form a clear idea of these processes, analo- gous and less complicated phenomena must previously be studied. The compound nature of the molecules of an organic body, and the phenomena presented by them when in relation with other matters, point out the true cause of these transformations. Evidence is afforded even by simple bodies, that in the formation of combinations, the force with which the combining elements adhere to one another is inversely proportional to the number of simple atoms in the compound molecule. Thus, protoxide of manganese by absorp- tion of oxygen is converted into the sesqui- oxide, the peroxide, manganic and hyper- manganic acids, the number of atoms of oxygen being augmented by J, by 1, by 2, and by 5. But all the oxygen contained in these compounds, beyond that which belongs to the protoxide, is bound to the manganese by a much more feeble affinity; a red heat causes an evolution of oxygen from the peroxide, and the manganic and hyperman- ganic acids cannot be separated from their bases without undergoing immediate decom- position. There are many facts which prove, that the most simple inorganic compounds are also the most stable, and undergo decompo- sition with the greatest difficulty, whilst those which are of a complex composition yield easily to changes and decompositions. The cause of this evidently is, that in pro- portion to the number of atoms which enter into a compound, the directions in which their attractions act will be more numerous. Whatever ideas we may entertain regard- ing the infinite divisibility of matter in general, the existence of chemical propor- tions removes every doubt respecting the pre- sence of certain limited groups or masses of matter which we have not the power of divid- ing. The particles of matter called equiva- lents in chemistry are not infinitely small, for they possess a weight, and are capable of arranging themselves in the most various ways, and of thus forming innumerable compound atoms. The properties of these compound atoms differ in organic nature, not only according to the form, but also in many instances according to the direction and place, which the simple atoms take in the compound molecules. When we compare the composition of organic compounds with inorganic, we are quite amazed at the existence of combina- tions, in one single molecule of which, ninety or several hundred atoms or equiva lents are united. Thus, the compound atom of an organic acid of very simple composi- tion, acetic acid for example, contains twelve equivalents of simple elements ; one atom of kinovic acid contains 33, 1 of sugar 36, 1 of amygdalin 90, and 1 of stearic acid 138 equivalents. The component parts of ani- mal bodies are infinitely more complex even than these. Inorganic compounds differ from organic in as great a degree in their other characters as in their simplicity of constitution. Thus, the decomposition of a compound atom of sulphate of potash is aided by numerous causes, such as the power of cohesion, or the capability of its constituents to form solid, insoluble, or at certain temperatures volatile compounds with the body brought into contact with it, and nevertheless a vast number of other substances produce in it not the slightest change. Now, in the de- composition of a complex organic atom, there is nothing similar to this. The empirical formula of sulphate of potash is SKO 4 . It contains only 1 eq. of sulphur, and 1 eq. of potassium. We may suppose the oxygen to be differently distri- buted in the compound, and by a decompo- sition we may remove a part or all of it, or replace one of the constituents of the com- pound by another substance. But we can- not produce a different arrangement of the atoms, because they are already disposed in the simplest form in which it is possible for them to combine. Now, let us compare the composition of sugar of grapes with the above : here 12 eq. of carbon, 12 eq. of hydrogen, and 12 eq. of oxygen, are united together, and we know that they are capa- ble of combining with each other in the most various ways. From the formula of sugar we might consider it either as a hy- drate of carbon, wood, starch, or sugar of milk, or farther, as a compound of ether with alchohol or of formic acid with sachul- min.* Indeed we may calculate almost all the known organic compounds destitute of nitrogen from sugar, by simply adding the elements of water, or by replacing any one of its elementary constituents by a different substance. The elements necessary to form these compounds are therefore contained in the sugar, and they must also possess the power of forming numerous combinations amongst themselves by their mutual attrac- tions. ' Now, when we examine what changes sugar undergoes when brought into contact with other bodies which exercise a marked * The black precipitate obtained by the action of hydrochloric acid on sugar. 92 AGRICULTURAL CHEMISTRY. influence upon it, we find, that these changes are not confined to any narrow limits, like those of inorganic bodies, but are in fact unlimited. The elements of sugar yield to every at- traction, and to each in a peculiar manner. In inorganic compounds, an acid acts upon a particular constituent of the body, which it decomposes, by virtue of its affinity for that constituent, and never resigns its proper chemical character, in whatever form it may be applied. But when it acts upon sugar, and induces great changes in that compound, it does this not by any superior affinity for a base existing in the sugar, but by disturb- ing the equilibrium in the mutual attraction of the elements of the sugar amongst them- selves. Muriatic and sulphuric acids, which differ so much from one another both in characters and composition, act in the same manner upon sugar. But the action of both varies according to the state in which they are ; thus they act in one way when dilute, in another when concentrated, and even dif- ferences in their temperature cause a change in their action. Thus sulphuric acid of a moderate degree of concentration converts sugar into a black carbonaceous matter, forming at the same time acetic and formic acids. But when the acid is more diluted, the sugar is converted into two brown sub- stances, both of them containing carbon and the elements of water. Again, when sugar is subjected to the action of alkalies, a whole series of different new products are obtained ; while oxidizing agents, such as nitric acid, produce from it carbonic acid, acetic acid, oxalic acid, formic acid, and many other products which have not yet been examined. If from the facts here stated we estimate the power with which the elements of sugar are united together^ and judge of the force of their attraction by the resistance which they offer to the action f bodies brought into contact with them, we must regard the atom of sugar as belonging to that class of compound "atoms, which exist only by the vis inertice of their elements. Its elements seem merely to retain passively the position and condition in which they had been placed, for we do not observe that they re- sist a change of this condition by their own mutual attraction, as is the case with sul- phate of potash. Now it is only such combinations as sugar, combinations therefore which possess a very complex molecule, which are capa- ble of undergoing the decompositions named fermentation and putrefaction. We have seen that metals acquire a power which they do not of themselves possess, namely, that of decomposing water and nitric acid, by simple contact with other metals in the act of chemical combination. We have also seen, that peroxide of hydro- gen and the persulphuret of the same ele- ment, in the act of decomposition, cause other compounds of a similar kind, but of which the elements are liuch more strongly combined, to undergo the same decomposi- tion, although they exert no chemical af- finity or attraction for them or their consti- tuents. The cause which produces these phenomena will be also recognised, by at- tentive observation, in those matters which excite fermentation or putrefaction. All bodies in the act of combination or decom- position have the property of inducing those processes ; or, in other words, of causing a disturbance of the statical equilibrium in the attractions of the elements of complex organic molecules, in consequence of which those elements group themselves anew, ac- cording to their special affinities. The proofs of the existence of this cause of action can be easily produced ; they are found in the characters of the bodies which effect fermentation and putrefaction, and in the regularity with which the distribution of the elements takes place in the subse- quent transformations. This regularity de- pends exclusively on the unequal affinity which they possess for each other in an isolated condition. The action of water on wood, charcoal, and cyanogen, the simplest of the compounds of nitrogen, suffices to il- lustrate the whole of the transformations of organic bodies ; of those in which nitrogen is a constituent, and of those in which it is absent. CHAPTER IV. ON THE TRANSFORMATION OF BODIES WHICH DO NOT CONTAIN NITROGEN AS A CONSTI- TUENT, AND OF THOSE IN WHICH IT IS PRESENT. WHEN oxygen and hydrogen combined in equal equivalents, as in steam, are con- ducted over charcoal, heated to the tempe- rature at which it possesses the power to enter into combination with one of these elements, a decomposition of steam ensues. An oxide of carbon (either carbonic oxide or carbonic acid) is under all circumstances formed, while the hydrogen of the water is liberated, or, if the temperature be sufficient, unites with the carbon, forming carburetted hydrogen. Accordingly, the carbon is shared between the elements of the water, the oxy- gen and hydrogen. Now a participation of this kind, but even more complete, is ob- served in every transformation, whatever be the nature of the causes by which it is effected. Acetic and meconic* acids suffer a true transformation under the influence of heat, that is, their component elements are dis- united, and form new compounds without any of them being singly disengaged. Acetic acid is converted into acetone and carbonic * An acid existing in opium, and named from the Greek for poppy. CHEMICAL TRANSFORMATIONS. 93 acid(C4 H3 O3=C3 H3 O + CO2,) and meconic acid into carbonic acid and kome- nic acid; whilst by the influence of a higher temperature, the latter is further decomposed into pyromeconic acid and carbonic acid. Now in these cases the carbon of the bo- dies decomposed is shared between the oxy- gen and the hydrogen ; part of it unites with the oxygen and forms carbonic acid, whilst the other portion enters into combination with the hydrogen, and an oxide of a carbo- hydrogen is formed, in which all the hy- drogen is contained. In a similar manner, when alcohol is exposed to a gentle red heal, its carbon is shared between the elements of the water an oxide of a carbo-hydrogen which con- tains all the oxygen, and some gaseous com- pounds of carbon and hydrogen being pro- duced. It is evident that during transformations caused by heat, no foreign affinities can be in play, so that the new compounds must result merely from the elements arranging themselves, according to the degree of their mutual affinities, into new combinations which are constant and unchangeable in the conditions under which they were origi- nally formed, but undergo changes when these conditions become different. If we compare the products of two bodies, similar in composition but different in properties, which are subjected to transformations by two different causes, we find that the mari- ner in which the atoms are transposed, is absolutely the same in both. In the transformation of wood in marshy soils, by what we call putrefaction, its car- bon is shared between the oxygen and hy- drogen of its own substance, and of the water carburetted hydrogen is consequently evolved, as well as carbonic acid, both of which compounds have an analogous corn- position (CH2, CO2.) Thus also in that transformation of sugar, which is called fermentation, its elements are divided into two portions ; the one, car- bonic acid, which contains $ of the oxygen of sugar ; and the other, alcohol, which con- tains all its hydrogen. In the transformation of acetic acid pro- duced by a red heat, carbonic acid, which contains 2-3 of the oxygen of the acetic acid is formed, and acetone, which contains al its hydrogen. It is evident from these facts, that the ele- ments of a complex compound are left to their special attractions whenever their equi- librium is disturbed, from whatever cause this disturbance may proceed. It appears also, that the subsequent distribution of the elements, so as to form new combinations always takes place in the same way, with this difference only, that the nature of th< products formed is dependent upon the num per of atoms of the elements which ente into action ; or, in other words, that the pro- ducts differ ad infinilum, according to the composition of the original substance. N THE TRANSFORMATION OF 10DIF.S CON- TAINING NITROGEN. When those substances are examined which are most prone to fermentation and utrefaction, it is found that they are all, vithout exception, bodies which contain itrogen. In many of these compounds, a ransposition of their elements occurs spon- aneously as soon as they cease to form part )f a living organism; that is, when they are drawn out of the sphere of attraction in which alone they are able to exist. There are, indeed, bodies destitute of ni- rogen, which possess a certain degree of liability only when in combination, but which are unknown in an isolated condition, >ecause their elements, freed from the power Dy which they were held together, arrange hemselves according to their own nalural altractions. Hypermanganic, maganic, and lyposulphurous acids, belong lo this class f substances, which however are rare. The case is very different wilh azolised Bodies. It would appear lhat there is some peculiarity in the nature of nitrogen, which ves its compounds the power to decom- pose spontaneously with so much facility. w, nitrogen is known to be the most in- different of all the elements; it evinces no particular attraction to any one of the simple bodies; and this character it preserves in all Is combinations, a character which explains the cause of ils easy separation from the matters with which it is united. It is only when the quantity of nitrogen exceeds a certain limit, that azotised com- pounds have some degree of permanence, as is ihe case with melamin, ammelin, &c. Their liability to change is also diminished, when the quantity of nitrogen is very small in proportion to that of the other elements with which it is united, so that their mutual attractions preponderate. This easy transposition of atoms is best seen in the fulminating silvers, in fulmi- nating mercury, in the iodide or chloride of nitrogen, and in all fulminating compounds. All other azotised substances acquire the same power of decomposition, when the elements of water are brought into play ; and indeed, the greater part of them are not capable of transformation, while this neces- sary condition to the transposition of their atoms is absent. Even the compounds of ni- trogen, which are most liable to change, such as those which are found in animal bodies, do not enter into a state of putrefac- faction when dry. The result of the known transformations of azotised substances proves that the water does not merely act as a medium in which motion is permitted to the elements in the act of transposition, but that its influence depends on chemical affinity. When the decomposition of such substances is effected with the assistance of water, their nitrogen is invariably liberated in the form of ammo- nia. This is a fixed rule without any excep- 94 AGRICULTURAL CHEMISTRY. tions, whatever may be the cause which produces the decompositions. All organic compounds containing nitrogen, evolve the whole of that element in the form of ammo- nia when acted on by alkalies. Acids, and increase of temperature, produce the same effect. It is only when there is a defi- ciency of water or its elements, that cyno- gen or other azotised compounds are pro- duced. From these facts it may be concluded, that ammonia is the most stable compound of nitrogen ; and that hydrogen and nitro- gen possess a degree of affinity for each other surpassing the attraction of the latter body for any other element. Already in considering the transforma- tions of substances destitute of nitrogen, we have recognised the great affinity of carbon for oxygen as a powerful cause for effecting the disunion of the elements of a complex organic atom in a definite manner. But car- bon is also invariably contained in azotised organic compounds, while the great affinity of nitrogen for hydrogen furnishes a new and powerful cause, facilitating the transpo- sition of their component parts. Thus, in the bodies which do not contain nitrogen we have one element, and in those in which that substance is present, two elements, which mutually share the elements of water. Hence there are two opposite affinities at play, which mutually strengthen each other's actions. Now we know, that the most powerful attractions may be overcome by the influ- ence of two affinities. Thus, a decomposi- tion of alumina may be effected with the greatest facility, when the affinity of char- coal for oxygen, and of chlorine for alumi- nium, are both put in action, although nei- ther of these alone has any influence upon it. There is in the nature and constitution of the compounds of nitrogen a kind of ten- sion of their component parts, and a strong disposition to yield to transformations, which effect spontaneously the transposition of their atoms on the instant that water or its elements are brought in contact with them. The characters of the hydrated cyanic acid, one of the simplest of all the com- pounds of nitrogen, are perhaps the best adapted to convey a distinct idea of the manner in which the atoms are disposed of in transformations. This acid contains ni- trogen, hydrogen, and oxygen, in such pro- portions, that the addition of a certain quan- tity of the elements of water is exactly suffi- cient to cause the oxygen contained in the water and acid to unite with the carbon and form carbonic acid, and the hydrogen of the water to combine with the nitrogen and form ammonia. The most favourable con- ditions for a complete transformation are, therefore, associated in these bodies, and it is well known, that the disunion takes place on the instant in which the cyanic acid and water are brought into contact,, the mixture being converted into carbonic acid and am monia, with brisk effervescence. This decomposition may be considered as the type of the transformations of all azo- lised compounds; it is putrefaction in its simplest and most perfect form, because the new products, the carbonic acid and ammo- nia are incapable of further transformations. Putrefaction assumes a totally different and much more complicated form, when the Rroducts, which are first formed undergo a irther change. In these cases the process consists of several stages, of which it is im- possible to determine when one ceases and the other begins. The transformations of cyanogen, a body composed of carbon and nitrogen, and the simplest of all the compounds of nitrogen, will convey a clear idea of the great variety of products which are produced in such a case : it is the only example of the putrefac- tion of an azotised body which has been at all accurately studied. A solution of cyanogen in water becomes turbid after a short time, and deposits a black, or brownish black matter, which is a combination of ammonia with another body, produced by the simple union of cyanogen with water. This substance is insoluble in water, and is thus enabled to resist further change. A second transformation is effected by the cyanogen being shared between the elements of the water, in consequence of which cyanic acid is formed by a certain quantity of the cyanogen combining with the oxygen of the water, while hydrocyanic acid is also formed by another portion of the cyanogen uniting with the hydrogen which was libe- rated. Cyanogen experiences a third transforma- tion, by which a complete disunion of its elements takes place, these being divided be- tween the constituents of the water. Oxa- lic acid is the one product of this disunion, and ammonia the other. Cyanic acid, the formation of which has been mentioned above, cannot exist in con- tact with water, being decomposed immedi- ately into carbonic acid and ammonia. The cyanic acid, however, newly formed in the decomposition of cyanogen, escapes this de- composition by entering into combination with the free ammonia, by which urea is produced. The hydrocyanic acid is also decomposed into a brown matter which contains hydro- gen and cyanogen, the latter in greater pro- portion than it does in the gaseous state. Oxalic acid, urea, and carbonic acid, are also formed by its decomposition, and formic acid and ammonia are produced by the decompo- sition of its radical. Thus, a substance into the composition of which only tv/o elements (carbon and nitrogen) enter, yields eight totally different products. Several of these products are formed by the transformation of the origin aJ body, its elements being shared between the CHEMICAL TRANSFORMATION'S. 95 constituents of -water ; others are produced in consequence of a further disunion of those first formed. The urea and carbonate of ammonia are generated by the combina- tion of two of the products, and in their for- mation the whole of the elements have as- sisted. These examples show, that the results of decomposition by fermentation or putrefac- tion comprehend very different phenomena. The first kind of transformation is, the transposition of the elements of one complex compound, by which new compounds are produced witfi or without the assistance of the elements of water. In the products newly formed in this manner, either the same proportions of those component parts which were contained in the matter before transformation, are found, or with them, an excess, consisting of the constituents of wa- ter which had assisted in promoting the dis- union of the elements. The second kind of transformation con- sists of the transpositions of the atoms of two or more complex compounds, by which the elements of both arrange themselves mutually into new products, with or with- out the co-operation of the elements of wa- ter. In this kind of transformations, the new products contain the sum of the con- stituents of all the compounds which had taken a part in the decomposition. The first of these two modes of decom- position is that designated fermentation, the second putrefaction ; and when these terms are used in the following pages, it will always be to distinguish the two processes above described, which are so different in their results. CHAPTER V. FERMENTATION OF SUGAR. THE peculiar decomposition which sugar suffers may be viewed as a type of all the transformations designated fermentation.* Thenard obtained from 100 grammes of cane-sugar 0.5262 of absolute alcohol. 100 parts of sugar from the cane yield, there- * When yeast is made into a thin paste with water, and I cubic centimetre of this mixture in- troduced into a graduated slass receiver filled with mercury, in which are already 19 grammes of a solution of cane sugar, containing 1 gramme of pure solid sugar: ids found after the mixture has been exposed for 24 hours to a temperature of from 20 to 25 C. (6877 F.,) that a volume of carbonic acid has been formed, which, at 0* C. (32 F.) and an atmospheric pressure indicated by 0.76 metre Bar. would be from 245 to 250 cubic centimetres. But to this quantity we must add 11 cubic centimetres of carbonic acid, with which the 11 grammes of liquid would be saturated, so that in all 255 259 cubic centimetres of carbonic acid are obtained. This volume of carbonic acid corresponds to from 0.503 to 0.5127 grammes by weight. fore, 103.89 parts of carbonic acid and alco- hol. The entire carbon in these products is equal to 42 parts, which is exactly the quan- tity originally contained in the sugar. The analysis of sugar from the cane, proves that it contains the elements of car- bonic acid and alcohol, minus I atom of water. The alcohol and carbonic acid pro- duced by the fermentation of a certain quan- tity of sugar, contain together one equivalent of oxygen and one equivalent of hydrogen, the elements, therefore, of one equivalent of water, more than the sugar contained. The excess of weight in the products is thus explained most satisfactorily ; it is ow- ing, namely, to the elements of water hav- ing taken part in the metamorphosis of the sugar. It is known that 1 atom of sugar contains 12 equivalents of carbon, both from the proportions in which it unites with bases, and from the composition of saccharic acid the product of its oxidation. Now none of these atoms of carbon are contained in the sugar as carbonic acid, because the whole quantity is obtained as oxalic acid, when sugar is treated with hypermanganate of Eotash (Gregory ;) and as oxalic acid is a >wer degree of the oxidation of carbon than carbonic acid, it is impossible to conceive that the lower degree should be produced from the higher, by means of one of the most powerful agents of oxidation which we possess. It can be also proved, that the hydrogen of the sugar does not exist in it in me form of alcohol, for it is converted into water and a kind of carbonaceous matter, when treated with acids, particularly with such as contain no oxygen ; and this manner of de- composition is never suffered by a com- pound of alcohol. Sugar contains, therefore, neither alcohol nor carbonic acid, so that these bodies must be produced by a different arrangement of its atoms, and by their union with the elements of water. In this metamorphosis of sugar, the ele- ments of the yeast, by contact with which its fermentation was effected, take no appre- ciable part in the transposition of the ele- ments of the sugar; for in the products resulting from the action, we find no com- ponent part of this substance. We may now study the fermentation of a vegetable juice, which contains not only saccharine matter, but also such substances as albumen and gluten. The juices of parsneps, beet-roots, and onions, are well adapted for this purpose. When such a juice is mixed with yeast at common temperatures, it ferments like a solution of sugar. Carbonic acid gas escapes from it with effervescence, and in the liquid, alcohol is found in quantity exactly corresponding to that of the sugar originally contained in the juice. But such a juice undergoes spon- taneous decomposition at a temperature of from 95 to 104 (35 40 C.) Gases 96 AGRICULTURAL CHEMISTRY. possessing an offensive smell are evolved in considerable quantity, and when the liquor is examined after the decomposition is com- pleted, no alcohol can be detected. The sugar has also disappeared, and with it all the azotised compounds which existed in the juice previously to its fermentation. Both were decomposed at the same time ; the nitro- gen of the azotised compounds remains in the liquid as ammoina, and, in addition to it, there are three new products, formed from the component parts of the juice. One of these is lactic acid, the slightly volatile com- pound found in the animal organism ; the other is the crystalline body which forms the principal constituent of manna ; and the third is a mass resembling gum-arabic, which forms a thick viscous solution with water. These three products weigh more than the sugar contained in the juice, even without calculating the weight of the gaseous pro- ducts. Hence they are not produced from the elements of the sugar alone. None of these three substances could be detected in the juice before fermentation. They must., therefore, have been formed by the inter- change of the elements of the sugar with those of the foreign substances also present. It is this mixed transformation of two or more compounds which receives the special name of putrefaction. YEAST OR FERMENT. When attention is directed to the condi- tion of those substances which possess the power of inducing fermentation and putre- faction in other bodies, evidences are found in their general characters, and in the man- ner in which they combine, that they all are bodies, the atoms of which are in the act of transposition. The characters of the remarkable matter which is deposited in an insoluble state during the fermentation of beer, wine, and vegetable juices, may first be studied. This substance, which has been called yeast or ferment, from the power which it possesses of causing fermentation in sugar, or saccharine vegetable juices, possesses all the characters of a compound of nitrogen in the state of putrefaction and eremacavsis. Like Avood in the state of eremacausis, yeast converts the oxygen of the surrounding air into carbonic acid, but it also evolves this gas from its own mass, like bodies in the state of putrefaction. (Colin.) When kept underwater, it emits carbonic acid, accompa- nied by gases of an offensive smell, (The- nard,) and is at last converted into a sub- stance resembling old cheese. (Proust.) But when its own putrefaction is completed, it has no longer the power of inducing fer- mentation in other bodies. The presence of water is quite necessary for sustaining the properties of ferment, for by simple pres- sure its power to^ excite fermentation is much diminished, and is completely de- stroyed by drying. Its action is arrested also by the temperature of boiling water, by al- cohol, common salt, an excess of sugar, oxide of mercury, corrosive sublimate, pyro- ligneous acid, sulphurous acid, nitrate of silver, volatile oils, and in short by all anti- septic substances. The insoluble part of the substance called ferment does not cause fermentation. For when the yeast from wine or beer is care- fully washed with water, care being taken that it is always covered with this fluid, the residue does not produce fermentation. The soluble part of ferment likewise does not excite fermentation. An aqueous infu- sion of yeast may be mixed with a solution of sugar, and preserved in vessels from which the air is excluded, without either experi- encing the slightest change. What then, we may ask, is the matter in ferment which ex- cites fermentation, if neither the soluble nor insoluble parts possess the power? This question has been answered by Colin in the most satisfactory manner. He has shown that in reality it is the soluble part. But before it obtains this power, the decanted infusion must be allowed to cool in contact with the air, and, to remain some time ex- posed to its action. When introduced into a solution of sugar in this state, it produces a brisk fermentation; but without previous exposure to the air, it manifests no such property. The infusion absorbs oxygen during- its exposure to the air, and carbonic acid may be found in it after a short time. Yeast produces fermentation in conse- quence of the progressive decomposition which it suffers from the action of air and water. Now when yeast is made to act on sugar, it is found, that after the transformation of the latter substance into carbonic acid and alcohol is completed, part of the yeast itself has disappeared. From 20 parts of fresh yeast from beer, and 100 parts of sugar, TJienard obtained, after the fermentation was completed, 13-7 parts of an insoluble residue, which dimi- nished to 10 parts when employed in the same way with a fresh portion of sugar. These ten parts were white, possessed of the properties of woody fibre, and had no farther action on sugar. It is evident, therefore, that during the fer- mentation of sugar by yeast, both of these substances suffer decomposition at the same time, and disappear in consequence. But if yeast be a body which excites fermenta- tion by being itself in a state of decomposi- tion, all other matters in the same condition should have a similar action upon sugar; and this is in reality the case. Muscle, urine, isinglass, osmazome, albumen, cheese, glia- dine, gluten, legumin, and blood, when in a state of putrefaction, have all the power of producing the putrefaction, or fermentation of a solution of sugar. Yeast, which by continued washing has entirely lost the pro- perty of inducing fermentation, regains it CHEMICAL TRANSFORMATIONS. 97 when its putrefaction has recommenced, in consequence of its being kept in a warm situation for some time. Yeast and putrefying animal and vegeta- ble matters act as peroxide of hydrogen does on oxide of silver, when they induce bodies with which they are in contact to enter into the same state of decomposition. The dis- turbance in the attraction of the constituents of the peroxide of hydrogen effects a disturb- ance in the attraction of the elements of the oxide of silver, the one being decomposed, on account of the decomposition of the other. Now if we consider the process of the fermentation of pure sugar, in a practical point of view, we meet with two facts of constant occurrence. When the quantity of ferment is too small in proportion to that of the sugar, its putrefaction will be com- pleted before the transformation of all the sugar is effected. Some sugar here remains undecomposed, because the cause of its transformation is absent, viz. contact with a body in a state of decomposition. But when the quantity of ferment pre- dominates, a certain quantity of it remains after all the sugar has fermented, its decom- position proceeding very slowly, on account of its insolubility in water. This residue of ferment is still able to induce fermentation when introduced into a fresh solution of su- gar, and retains the same power until it has passed through all the stages of its own transformation. Hence a certain quantity of yeast is necessary in order to effect the transformation of a certain portion of sugar, not because it acts by its quantity in increas- ing any affinity, but because its influence depends solely on its presence, and its pre- sence is necessary, until the last atom of sugar is decomposed. These facts and observations point out the existence of a new cause, which effects combinations and decompositions. This cause is the action which bodies in a state of combination or decomposition exercise upon substances, the component parts of which are united together by a feeble affinity. This action resembles a peculiar power, at- tached to a body in the state of combination or decomposition, but exerting its influence beyond the sphere of its own attractions. We are now able to account satisfactorily for many known phenomena. A large quantity of hippuric acid may be obtained from the fresh urine of a horse, by the addition of muriatic acid ; but when the urine has undergone putrefaction, no trace of it can be discovered. The urine of man contains a considerable quantity of urea; but when the urine putrefies, the urea en- tirely disappears. When urea is added to a solution of sugar in the state of fermentation, it is decomposed into carbonic acid and am- monia. No asparagin can be detected in a putrefied infusion of asparagin, liquorice- root, or the root of marshrn allow (Jilthcea officinalis. 13 It has already been mentioned, that the strong affinity of nitrogen for hydrogen, and that of carbon for oxygen, are the cause of the facility with which the elements of azo- tised compounds are disunited ; those affini- ties aiding each other, inasmuch as by vir- tue of them different elements of the com- pounds strive to take possession of the dif- ferent elements of water. Now since it is found that no body destitute of nitrogen pos- sesses, when pure, the property of decom- posing spontaneously whilst in contact with water, we must ascribe this property which azotised bodies possess in so eminent a de- gree, to something peculiar in the nature of the compounds of nitrogen, and to their con- stituting, in a certain measure, more highly organized atoms. Every azotised constituent of the animal or vegetable organism runs spontaneously | into putrefaction, when exposed to moisture and a high temperature. Azotised matters are, accordingly, the only causes of fermentation and putrefaction in vegetable substances. Putrefaction, on account of its effects, as a mixed transformation of many different i substances, may be classed with the most powerful processes of deoxidation, by which ; the strongest affinities are overcome." When a solution of gypsum in water is mixed with a decoction of sawdust, or any- other organic matter capable of putrefaction, and preserved in well-closed vessels, it is found after some time, that the solution con- tains no more sulphuric acid, but in its place carbonic and free hydro-sulphuric acid, between which the lime of the gypsum is shared. In stagnant water containing sulphates in solution, crystallised pyrites is observed to form on the decaying roots. Now we know that in the putrefaction of wood under water, when air therefore is ex- cluded, a part of its carbon combines with the oxygen of the water, as well as with the oxygen which the wood itself contains; whilst its hydrogen and that of the decom- posed water are liberated either in a pure state, or as carburetted hydrogen. The products of this decomposition are of the same kind as those generated when steam is conducted over red-hot charcoal. It is evident, that if with the water a sub- stance containing a large quantity of oxygen, such as sulphuric acid, be also "present, the matters in the state of putrefaction will make use of the oxygen of that substance as well as that of the water, in order to form car- bonic acid ; and the sulphur and hydrogen being set free will combine whilst in the ntscent state, producing hydrosulphuric acid, which will be again decomposed if metallic oxides be present; and the resu.ts of this second decomposition will be wa;er and metallic sulphurets. The putrefied leaves of woad (Isatis tinc- toria,) in contact with indigo-blue, water, and alkalies, suffer farther decomposition, and the indigo is deoxidised and dissolved. AGRICULTURAL CHEMISTRY. The mannite formed by the putrefaction of beet-roots and other plants which contain Bugar, contains the same number of equiva- lents of carbon and hydrogen as the sugar of grapes, but two atoms less of oxygen j and it is highly probable that it. is produced from sugar of" grapes, contained in those plants, in precisely the same manner as in- digo-blue is converted into deoxidised white indigo. During the putrefaction of gluten, car- bonic acid and pure hydrogen gas are evolved; phosphate, acetate,, caseate, and laetate of ammonia being at the same time produced in such quantity, that the further decomposition of the gluten ceases. But when the supply of water is renewed, the decomposition begins again, and in addition to the salts just mentioned, carbonate of am- monia and a white crystalline matter re- sembling mica (caseous oxide) are formed, together with hydrosulphate of ammonia, and a mucilaginous substance coagulable by chlorine. Lactic acid is almost always produced by the putrefaction of organic bodies. We may now compare fermentation and putrefaction with the decomposition which organic compounds suffer under the influ- ence of a high temperature. Dry distilla- tion would appear to be a process of com- bustion or oxidation going on in the interior of a substance, in which a part of the car- bon unites with all or part of the oxygen of the compound, while other new compounds containing a large proportion of hydrogen are necessarily produced. Fermentation may be considered as a process of combus- tion or oxidation of a similar kind, taking place in a liquid between the elements of the same matter, at a very slightly elevated temperature; and putrefaction as a process of oxidation, in which the oxygen of all the substances present comes into play. CHAPTER VI. EREMACAUSIS, OR DECAY. IN organic nature, besides the processes of decomposition named fermentation and putrefaction, another and not less striking class of changes occurs, which bodies suf- fer from the influence of the air. This is the act of gradual combination of the com- bustible elements of a body with the oxygen of the air ; a slow combustion or oxidation, to which we shall apply the term of ere- macausis. The conversion of wood into humus, the formation of acetic acid out of alcohol, ni- trification, and numerous other processes, are of this nature. Vegetable juices of every kind, parts of animal and vegetable substances, moist sawdust, blood, &c., can- not be exposed to the air, without suffering immediately a progressive change of colour and properties, during which oxygen is ab sorbed. These changes do not take place when water is excluded, or when the sub- stances are exposed to the temperature of 32, and it has been observed that different bodies require different degrees of heat, in order to effect the absorption of oxygen, and, consequently, their eremacausis. The property of suffering this change is pos- sessed in the highest degree by substances containing nitrogen. When vegetable juices are evaporated by a gentle heat in the air, a brown or brown- ish-black substance is precipitated as a pro- duct of the action of oxygen upon them. This substance, which appears to possess similar properties from whatever juice it is obtained, has received the name of extractive mattery it is insoluble or very sparingly soluble in water, but is dissolved with facil- ity by alkalies. By the action of air on solid animal or vegetable matters, a similar pulverulent brown substance is formed, and is known by the name of humus. The conditions which determine the com- mencement of eremacausis are of various kinds. Many organic substances, particu- larly such as are mixtures of several more simple matters, oxidise in the air when simply moistened with water; others not until they are subjected to the action of al- kalies ; but the greatest part of them undergo this state of slow combustion or oxidation, when brought in contact with other decay- ing matters^ The eremacausis of an organic matter is retarded or completely arrested by all those substances which prevent fermentation or putrefaction. Mineral acids, salts of mer- cury, aromatic substances, empyreumatic oils, and oil of turpentine, possess a simi- lar action in this respect. The latter sub- stances have the same effect on decaying bodies as on phosphuretted hydrogen, the spontaneous inflammability of which they destroy. Many bodies which do not decay when moistened with water, enter into eremacau- sis when in contact with an alkali. Gallic acid, hsemalin,, and many other compounds, may be dissolved in water and yet remain unaltered ; but if the smallest quantity of a free alkali is present, they acquire the pro- perty of attracting oxygen, and are con- verted into a brown substance like humus, evolving very frequently at the same time carbonic acid. (Chevreul.) A very remarkable kind of eremacausis takes place in many vegetable substances, when they are exposed to the influence of air, water, and ammonia. They absorb oxygen very rapidly, and form splendid violet or red- coloured liquids, as in the case of orcin and erythrin. They now contain an azotised substance, not in the form of ammonia. All these facts show that the action of oxygen seldom affects the carbon of decay- ing substances, and this corresponds exactly EREMACAUSIS OR DECAY. 99 .0 what happens in combustion at high tem- peratures. It is well known, for example, that when no more oxygen is admitted to a compound of carbon and hydrogen than is sufficient to combine with its hydrogen, the carbon is not burned, but is separated as lamp-black ; while, if the quantity of oxygen is not sufficient even to consume all the hy- drogen, new compounds are formed, such as naphthalin and similar matters, which contain a smaller proportion of hydrogen than those compounds of carbon and hydro- gen which previously existed in the com- bustible substance. There is no example of carbon combining directly with oxygen at common tempera- tures, "but numerous facts show that hydro- gen, in certain states of condensation, pos- sesses that property. Lamp-black which has been heated to redness may be kept in contact with oxygen gas, without forming carbonic acid ; but lamp-black, impregnated with oils which contain a large proportion of hydrogen, gradually becomes warm, and inflames spontaneously. The spontaneous inflammability of the charcoal used in the fabrication of gunpowder has been correctly ascribed to the hydrogen which it contains in considerable quantity ; for during its re- duction to powder, no trace of carbonic acid can be detected in the air surrounding it; it is not formed until the temperature of the mass has reached a red heat. The heat which produces the inflammation is there- fore not caused by the oxidation of the car- bon. The substances which undergo erema- causis may be divided into two classes. The first class comprehends those substances which unite with the oxygen of the air, without evolving carbonic acid; and the second, such as emit carbonic acid by ab- sorbing oxygen. When tne oil of bitter almonds is exposed to the air, it absorbs two equivalents of oxygen, and is converted into benzoic acid ; but half of the oxygen absorbed combines with the hydrogen of the oil, and forms water, which remains in union with the anhydrous benzoic acid.* * According to the experiments of Dobereiner, 100 parts of pyrogallic acid absorbs 38'09 parts of oxygen when in contact with ammonia and water ; the acid being changed in consequence of this ab- sorption into a mouldy substance, which contains less oxygen than the acid itself. It is evident that the substance which is formed is not a higher oxide ; and it is found, on comparing the quantity of the oxygen absorbed with that of the hydrogen contained in the acid, that they are exactly in the proportions for forming water. When colourless orcinis exposed together with ammonia to the contact of oxygen gas, the beau- tiful red-coloured orcein is produced. Now, the only changes which take place here are, that the absorption of oxygen by the elements of orcin and ammonia causes the formation of water ; 1 equivalent of orcin CIS H12 O8, and 1 equivalent ot ammonia NH3, absorbs equivalents of oxygen, and 5 equivalents of water are produced, the com- position of orcin being CIS HlO 08 N. (Dumas.) But, although it appears very probable that the oxygen acts primarily and princi- pally upon hydrogen, the most combustible constituent of organic matter in the state of decay ; still it cannot thence be concluded that the carbon is quite devoid of the power to unite with oxygen, when every particle of it is surrounded with hydrogen, an ele- ment with which the oxygen combines with greater facility. We know, on the contrary, that although nitrogen cannot be made to combine with oxygen directly, yet it is oxidized and forms nitric acid, when mixed with a large quan- tity of hydrogen, and burned in oxygen gas. In this case its affinity is evidently increased by the combustion of the hydrogen, which is in fact communicated to it. It is con- ceivable, that in a similar manner, the car- bon maybe directly oxidised in several cases, obtaining from its contact with hydrogen in eremacausis a property which it does not itself possess at common temperatures. But the formation of carbonic acid during the eremacausis of bodies containing hydrogen, must in most cases be ascribed to another cause. It appears to be formed in a man- ner similar to the formation of acetic acid, by the eremacausis of saliculite of potash.* An alkaline solution of haematin being exposed to an atmosphere of oxygen, O2 grm. absorb 28*6 cubic centimetres of oxy- gen gas in twenty-four hours, the alkali ac- quiring at the same time 6 cubic centimetres of carbonic acid. (Chevreul.) But these 6 cubic centimetres of carbonic acid contain only an equal volume of oxygen, so that it is certain from this experiment that | of the oxygen absorbed have not united with the carbon. It is highly probable, that during the oxidation of the hydrogen, a portion of the carbon had united with the oxygen con- tained in the haematin, and had separated from the other elements as carbonic acid. The experiments of De Saussure upon the decay of woody fibre show that such a separation is quite possible. Moist woody fibre evolved one volume of carbonic acid for every volume of oxygen which it ab- sorbed. It has just been mentioned that carbonic acid contains its own volume of oxygen. Now, woody fibre contains carbon, and the elements of water, so that the result of the action of oxygen upon it is exactly the same as if pure charcoal had combined directly with oxvgen. But the characters of woody fibre show, that the elements of water are not contained in it in the form of water; for, were this the case, starch, sugar, and gum must also be considered as hydrates of carbon. In this case it is evident, that the oxygen absorbed has united merely with the hydrogen. * This salt, when exposed to a moist atmo- sphere, absorbs 3 atoms of oxygen ; melanic acid is produced, a body resembling humus, in conse- quence of the formation of which, the elements of 1 atom of acetic acid are separated from the saliculous acid. 100 AGRICULTURAL CHEMISTRY. But if the hydrogen does not exist in woody fibre in the form of water, the direct ! oxidation of the carbon cannot be considered as at all probable, without rejecting all the facts established by experiment regarding the process of combustion at low tempera- tures. If we examine the action of oxygen upon a substance containing a large quantity of hydrogen, such as alcohol, we find most distinctly, that the direct formation of car- bonic acid is the last stage of its oxidation, and that it is preceded by a series of changes, the last of which is a complete combustion of the hydrogen. Aldehyde, acetic, formic, oxalic, and carbonic acids, form a connected, chain, of products arising from the oxidation of alcohol; and the successive changes which this fluid expeiiences from the action of oxygen may be readily traced in them. Aldehyde is alcohol minus hydrogen; acetic acid is formed by the direct union of alde- hyde with oxygen. Formic acid and water are formed by the union of acetic acid with oxygen. When all the hydrogen is removed from this formic acid, oxalic acid is pro- duced ; and the latter acid is converted into carbonic acid by uniting with an additional portion of oxygen. All these products appear to be formed simultaneously, by the action of oxidising agents on alcohol ; but it can scarcely be doubted, that the forma- tion of the last product, the carbonic acid, does not take place until all the hydrogen has been abstracted. The absorption of oxygen by drying oils certainly does not depend upon the oxida- tion of their carbon ; for in raw nut-oil, for example, which was not free from mucilage and other substances, only twenty-one vo- lumes of carbonic acid were formed for every 146 volumes of oxygen gas absorbed. It must be remembered, that combustion or oxidation at low temperatures produces results quite similar to combustion at high temperatures wilh limited access of air. The most combustible element of a compound, which is exposed to the action of oxygen, must become oxidised first, for its superior combustibility is caused by its being enabled to unite with oxygen at a temperature at which the other elements cannot enter into that combination ; this property having the game effect as a greater affinity. The combustibility of potassium is no measure for its affinity for oxygen ; we have reason to believe that the attraction of mag- nesium and aluminium for oxygen is greater than that of potassium for the same element; but neither of those metals oxidises either in air or water at common temperatures, whilst potassium decomposes water with great violence, and appropriates its oxygen. Phosphorus and hydrogen combine with oxygen at ordinary temperatures, the first in moist air, the second when in contact with finely-divided platinum ; while char- coal requires a red heat before it can enter into combination with oxygen. It is evi- dent that phosphorus and hydrogen are more combustible than charcoal, that is, that their affinity for oxygen at common tempera- tures is greater ; and this is not the less cer- tain, because it is found, that carbon in cer- tain other conditions shows a much greater affinity for oxygen than either of those sub- stances. In putrefaction, the conditions are evi- dently present, under which the affinity of arbon for oxygen comes into play; neither expansion, cohesion, nor the gaseous state, opposes it, whilst in .eremacausis all these restraints have to be overcome. The evolution of carbonic acid, during the decay or eremacausis of animal or vege- table bodies which are rich in hydrogen, must accordingly be ascribed to a transposi- tion of the elements or disturbance in their attractions, similar to that which gives rise to the formation of carbonic acid in the pro- cesses of fermentation and putrefaction. The eremacausis of such substances is, therefore, a decomposition analogous to the putrefaction of azotised bodies. For in these there are two affinities at play ; the affinity of nitrogen for hydrogen, and that of carbon for oxygen, and both facilitate the disunion of the elements. Now there are two affini- ties also in action in those bodies which de- cay with the evolution of carbonic acid. One of these affinities is the attraction of the oxygen of the air for the hydrogen of the substance, which corresponds to the attrac- tion of nitrogen for the same element ; and the other is the affinity of the carbon of the substance for its oxygen, which is constant under all circumstances. When wood putrefies in marshes, carbon ancj oxygen are separated from its elements in the form of carbonic acid, and hydrogen in the form of carburetted hydrogen. But when wood decays or putrefies in the air, its hydrogen does not combine with carbon, but with oxygen, for which it has a much greater affinity at common temperatures. Now it is evident from the complete simi- larity of these processes, that decaying and putrefying bodies can mutually replace one another in their reciprocal actions. All putrefying bodies pass into the state of decay, when exposed freely to the air, and all decaying matters into that of putre- faction when air is excluded. All bodies, likewise, in a state of decay are capable of inducing putrefaction in other bodies in the same manner as putrefying bodies them- selves do. CHAPTER VII. EREMACAUSIS OR DECAY OF BODIES DESTI- TUTE OF NITROGEN: FORMATION OF ACETIC ACID. ALL those substances which appear to possess the property of entering spontane- EREMACAUSIS OR DECAY. 101 ously into fermentation and putrefaction, do ' oxygen. The oxygen acts here in a similar not in reality suffer those changes without manner to the friction or motion which af- some previous disturbance in the attraction fects the mutual decomposition of two salts, of their elements. Eremacausis always ore- j the crystallization of salts from their solution, cedes fermentation and putrefaction, and it or the explosion of fulminating mercury. It s is not until after the absorption of a certain \ causes the state of rest to be converted into s. quantity of oxygen that the signs of a trans- j a state of motion. formation in the interior of the substances I When this condition of intestine motion show themselves. I is once excited, the presence of oxygen is C" It is a very general error to suppose that no longer necessary. The smallest particle organic substances have the power of un- j of an azotised body in this act of decompo- ig change spontaneously, without the ; sition exercises an influence upon the parti- aid of an external cause. When they are j -tides in contact with it, and the state of not in a state of change, it is necessary, be- j motion is thus propagated through the sub- fore they can assume that state, that the existing equilibrium of their elements should be disturbed ; and the most common cause | of this disturbance is undoubtedly the atmo- sphere which surrounds all bodies. The juices of the fruit or other part of a plant which very readily undergo decompo- stance. The air may now be completely excluded, but the fermentation or putrefac- tion proceeds uninterruptedly to its comple- tion. It has been remarked that the mere contact of carbonic acid is sufficient to pro- duce fermentation in the juices of several fruits. The contact of ammonia and alkalies in sition, retain their properties unchanged as i lorn contact cells or organs in which they are contained \ commencement of eremacausis ; for their resist the influence of the air. It is not i presence causes many substances to absorb ig as they are protected from immediate j general may be mentioned amongst the 11 tact with the air, that is, as long as the ! chemical conditions which determine the oxygen and to decay, in which neither oxy- gen nor alkalies alone produce that change. Thus alcohol does not combine with the oxygen of the air at common temperatures. But a solution of potash in alcohol absorbs oxygen with much rapidity, and acquires a brown colour. The alcohol is found after a short time to contain acetic acid, formic acid, and the products of the decomposition of aldehyde by alkalies, including aldehyde resin, which gives the liquid a brown colour. The most general condition for the pro- duction of eremacausis in organic matter is contact with a body already in the state of eremacausis or putrefaction. We have here an instance of true contagion; for the com- munication of the state of combustion is in reality the effect of the contact. It is decaying wood which causes fresh wood around it to assume the same condi- tion, and it is the very finely divided woody fibre in the act of decay which in moistened gall-nuts converts the tannic acid with such rapidity into gallic acid. A most remarkable and decided example of this induction of combustion has been observed by De Saussure. It has already been mentioned, that moist woody fibre, cotton, silk, or vegetable mould, in the act of fermentation or putrefaction, converts oxygen gas which may surround it into car- bonic acid, without change of volume. Now, De Saussure added a certain quantity of hy- upon opening the vessels after this long.rmed. The yeast now deposited has lost the property of exciting ordinary fermenta- tion, but it produces the other process even at a temperature of 50 F. In wort subjected to fermentation, at a low temperature, with this kind of yeast, the condition necessary for the transforma- tion of the sugar is the presence of that yeast ; but for the conversion of gluten into ferment by a process of oxidation, some- thing more is required. When the power of gluten to attract oxy- gen is increased by contact with precipitated yeast in a state of decay, the unrestrained access of air is the only other condition necessary for its own conversion into the same state of decay, that is for its oxidation. We have already seen that the presence of free oxygen and gluten are conditions which determine the eremacausis of alcohol and its conversion into acetic acid, but they are incapable of exerting this influence at low temperatures. A low temperature retards the slow combustion of alcohol, while the gluten combines spontaneously with the ^ oxygen of the air, just as sulphuric acid does when dissolved in water. Alcohol un- dergoes no such change at low temperatures, but during the oxidation of the gluten in contact with it, is placed in the same condi- tion as the gluten itself when sulphurous acid is added to the wine in which it is con- tained. The oxygen of the air unites both with the gluten and alcohol of wine not treated with sulphurous acid ; but when this acid is present it combines with neither of them, being altogether absorbed by the acid. The same thing happens in the peculiar pro- cess of fermentation adopted in Bavaria. The oxygen of the air unites only with the gluten and not with the alcohol, although it would have combined with both at higher tempe- ratures, so as to form acetic acid. Thus, then, this remarkable process of fermentation with the precipitation of a mu- cous-like ferment consists of a simultaneous putrefaction and decay in the same liquid. The sugar is in the state of putrefaction, and the gluten in that of decay. Appert's method of preserving food, and this kind of fermentation of beer, depend on the same principle. In the fermentation of beer after this man- ner, all the substances capable of decay are separated from it by means of an unre- strained access of air, while the temperature Is kept sufficiently low to prevent the alco- hol from combining with oxygen. The re- moval of these substances diminishes the tendency of the beer to become acescent, or in other words, to suffer a farther transforma- tion. In Appert's mode of preserving food, oxygen is allowed to enter into combination with the substance of the food, at a tempe- rature at which decay, but neither putrefac- tion nor fermentation, can take place. With the subsequent exclusion of the oxygen and the completion of the decay, every cause which could effect farther decomposition of the food is removed. The conditions for putrefaction are rendered insufficient in both cases; in the one by the removal of the substances susceptible of decay, in the other by the exclusion of the oxygen which would effect it. It has been stated to be uncertain, whether gluten during its conversion into common yeast, that is, into the insoluble state in which it separates from fermenting liquids, really combines directly with oxygen. If it does combine with oxygen, then the difference between gluten and ferment would be, that the latter would contain a larger proportion of oxygen. Now it is very difficult to as- certain this, and even their analyses cannot decide the question. Let us consider, for example, the relations of alloxan and allox- antin* to one another. Both of these bodies contain the same elements as gluten, although in different proportions. Now they are known to be convertible into each other, by oxygen being absorbed in the one case, and in the other extracted. Both are composed of ab- solutely the same elements, in equal pro- portions ; with the single exception, that al- loxantin contains 1 equivalent of hydrogen more than alloxan. When alloxantin is treated with chlorine and nitric acid, it is converted into alloxan, into a body, therefore, which is alloxantin minus 1 equivalent of hydrogen. If on the other hand a stream of sulphuretted hydro- gen is conducted through alloxan, sulphur is precipitated, and alloxantin produced. It may be said, that in the first case hydrogen is abstracted, in the other added. But it would be quite as simple an explanation, if we considered them as oxides of the same radical: the alloxan being regarded as a combination of a body composed of C N H> O with 2 equivalents of water, and al- loxantin as a combination of 3 atoms of water, with a compound consisting of C* N H O'. The conversion of alloxan into alloxantin would in this case result from its eight atoms of oxygen being reduced to seven, while alloxan would be formed out of alloxantin, by its combining with an ad- ditional atom of oxygen. Now, oxides are known which combine with water, and present the same pheno- mena as alloxan and alloxantin. But no * Compounds obtained by the action of nitric acid on uric acid. FERMENTATION OF BEER 109 compounds of hydrogen are known which form hydrates; and custom, which rejects all dissimilarity until the claim to peculiarity is quite proved, leads us to prefer an opinion, for which there is no farther foundation than that of analogy. The woad (Isatis tinctarid) and several species of the Nerivm contain a substance similar in many respects to gluten, which is deposited as indigo blue, when an aqueous infusion of the dried leaves is ex- posed to the action of the air. Now it is very doubtful whether the blue insoluble in- digo is an oxide of the colourless soluble indigo, or the latter a combination of hydro- gen Avith the indigo blue. Dumas has found the same elements in both, except that the soluble compound contained 1 equivalent of hydrogen more than the blue. "in the same manner the soluble gluten may be considered a compound of hydrogen, which becomes ferment by losing a certain quantity of this element when exposed to die action of the oxygen of the ah* under fa- vourable circumstances. At all events, it is certain that oxygen is the cause of the in- soluble condition of gluten ; for yeast is not deposited on keeping wine, or during the fermentation of Bavarian beer, unless oxy- gen has access to the fluid. Now whatever be the form in which the oxygen unites with the gluten whether it combines directly with it or extracts a por- tion of its hydrogen, forming water the products formed in the interior of the liquid, in consequence of the conversion of the glu- ten into ferment, will still be the same. Let us suppose that gluten is a compound of an- other substance with hydrogen, then this hydrogen must be removed during the ordi- nary fermentation of must and wort, by combining with oxygen, exactly as in the conversion of alcohol into aldehyd by ere- macausis. In both cases the atmosphere is excluded ; the oxygen cannot, then, be derived from the air, neither can it be supplied by the elements of water, for it is impossible to sup- pose that the oxygen will separate from the hydrogen of water, for the purpose of unit- ing with the hydrogen of gluten, in order again to form water. The oxygen must, therefore, be obtained from the elements of sugar, a portion of which substance must, in order to the formation of ferment, undergo a different decomposition from that which produces alcohol. Hence a certain part of the sugar will not be converted into carbonic acid and alcohol, but will yield other pro- ducts containing less oxygen than sugar it- self contains. These products, as has already been mentioned, are the cause of the great difference in the qualities of fermented li- quids, and particularly in the quantity of alcohol which they contain. Must and wort do not, therefore, in ordi- nary fermentation, yield alcohol in propor- tion to the quantity of sugar which they hold in solution, a part of the sugar being employed in the conversion of glute/i into ferment, and not in the formation of alcohol. But in the fermentation of Bavarian beer all the sugar is expended in the production of alcohol; and this is especially the case when- ever the transformation of the sugar is not accompanied by the formation of yeast. It is quite certain that in the distilleries of brandy from potatoes, where no yeast is formed, or only a quantity corresponding to the malt which has been added, the propor- tion of alcohol and carbonic acid obtained during the fermentation of the mash corre- sponds exactly to that of the carbon contained in the starch. It is also known that the volume of carbonic acid evolved during the fermentation of beet-roots gives no exact in- dication of the proportion of sugar contained in them, for less carbonic acid is obtained than the same quantity of pure sugar would yield. Beer obtained by the mode of fermenta- tion adopted in Bavaria contains more alco- hol, and possesses more intoxicating proper- ties, than that made by the ordinary method of fermentation, when the quantities of malt used are the same. The strong taste of the former beer is generally ascribed to its containing carbonic acid in larger quantity, and in a state of more intimate combination ; but this opinion is erroneous. Both kinds of beer are, at the conclusion of the fermen- tation, completely saturated with carbonic acid, the one as much as the other. Like all other liquids, they both must retain such a portion of the carbonic acid evolved as corresponds to their power of solution, that is, to their volumes. The temperature of the fluid during fer- mentation has a very important influence on the quantity of alcohol generated. It has been mentioned, that the juice of beet- roots allowed to ferment at from 86 to 95 (30 to 35 C.) yields no alcohol; and that afterwards, in the place of the sugar, man- nite, a substance incapable of fermentation, and containing very little oxygen, is found, together with lactic acid and mucilage. The formation of these product? diminishes in proportion as the temperature is lower. But in vegetable juices, containing nitrogen, it is impossible to fix a limit, where the trans- formation of the sugar is undisturbed by any other process of decomposition. It is known that in the fermentation of Bavarian beer the action of the oxygen of the air, and the low temperature, cause complete transformation of the sugar into alcohol ; the cause which would prevent that result, namely, the extraction of the oxygen of part of the sugar by the gluten, in its conversion into ferment, being avoided by the introduction of oxygen from without. The quantity of matters in the act of transformation is naturally greatest at the beginning of the fermentation of must and wort; and all the phenomena which accom- pany the process, such as evolution of gas, and heat, are best observed at that time. These signs of the changes proceeding in 110 AGRICULTURAL CHEMISTRY. the fluid diminish when the greater part of the sugar has undergone decomposition ; but they must cease entirely before the pro- cess can be regarded as completed. The less rapid process of decomposition which succeeds the violent evolution of gas, continues in wine and beer until the sugar has completely disappeared ; and hence it is observed, that the specific gravity of the liquid diminishes during many months. This slow fermentation, in most cases, re- sembles the fermentation of Bavarian beer, the transformation of the dissolved sugar heing in part the result of a slow and con- tinued decomposition of the precipitated yeast; but a complete separation of the azotised substances dissolved in it cannot take place when air is excluded.* Neither alcohol alone, nor hops, nor in- deed both together, preserve beer from be- coming acid. The better kinds of ale and porter in England are protected from acidity, but at the loss of the interest of an immense capital. They are placed in large closed wooden vessels, the surfaces of which are covered with sand. In these they are al- lowed to lie for several years, so that they are treated in a manner exactly similar to wine during its ripening. A gentle diffusion of air takes place through the pores of the wood, but the quan- tity of azotised substances being very great in proportion to the oxygen which enters, they consume it, and prevent its union with the alcohol. But the beer treated in this way does not keep for two months without acidifying, if it be placed in smaller vessels, to which free access of the air is permitted. CHAPTER X. DECAY OF WOODY FIBRE. THE conversion of woody fibre into the substances termed humus and mould is, on account of its influence on vegetation, one of the most remarkable processes of decom- position which occur in nature. Decay is not less important in another point of view ; for, by means of its influ- ence on dead vegetable matter, the oxygen which plants retained during life is again restored to the atmosphere. The decomposition of woody fibre is ef- fected in three forms, the results of which * The great influence which a rational manage- ment of fermentation exercises upon the quality of beer is well known in several of the German states. In the grand-duchy of Hesse, for example, a considerable premium is offered for the prepa- ration of beer, according to the Bavarian method ; and the premium is to be adjudged to any one who can prove that the beer brewed by him has lain for six months in the store-vats without be- coming acid. Hundreds of casks of beer became changed to vinegar before an empirical knowledge of those conditions was obtained, the influence of which is rendered intelligible by the theory, are different, so that it is necessary to con- sider each separately. The first takes place when it is in the moist condition, and subject to free uninter- rupted access of air; the second occurs when the air is excluded; and the third when the wood is covered with water, and in contact with putrefying organic matter. It is known that woody fibre may be kept under water, or in dry air, for thousands of years without suffering any appreciable change; but that when brought into contact with air, in the moist condition, it converts the oxygen surrounding it into the same volume of carbonic acid, and is itself gradually changed into a yellowish brown, or black matter, of a loose texture.* It has already been mentioned, that pure woody fibre contains carbon and the ele- ments of water. Humus, however, is not produced by the decay of pure woody fibre, but by that of wood which contains foreign soluble and insoluble organic substances, besides its essential constituents. The relative proportion of the component elements are, on this account, different in oak wood and in beech, and the composition of both of these differs very much from woody fibre, which is the same in all vege- tables. The difference, however, is so tri- vial, that it may be altogether neglected in the consideration of the questions which will now be brought under discussion; be- sides, the quantity of the foreign substances is not constant, but varies according to the season of the year. According to the careful analysis of Gay- Lussac and Thenard, 100 parts of oak wood, dried at 212 (100 C.,) from which all soluble substances had been extracted by means of water and alcohol, contained 52'53 parts of carbon, and 47*47 parts of hydrogen and oxygen, in the same propor- tion as they are contained in water. Now it has been mentioned that moist wood acts in oxygen gas exactly as if its carbon combined directly with oxygen, and that the products of this action are carbonic acid and humus. If the action of the oxygen were confined to the carbon of the wood, and if nothing but carbon were removed from it, the re- maining elements would necessarily be found in the humus, unchanged except in the particular of being combined with less carbon. The final result of the action would therefore be a complete disappearance of the carbon, whilst nothing but the elements of water would remain. But when decaying wood is subjected to examination in different stages of its decay, * According to the experiments of DC Saussure, 240 parts of dry sawdust of oak wood convert 10 cubic inches of oxygen into the same quantity of carbonic acid, which contains 3 parts, by weight, of carbon ; while the weight of the sawdust is di- minished by 15 parts. Hence, 12 parts, by weight, of water, are at the same time separated from the elements of the wood, DECAY OP WOODY FIBRE. Ill the remarkable result is obtained, that the | proportion of carbon in the different products augments. Consequently, if we did not take into consideration the evolution of car- bonic acid under the influence of the air, the conversion of wood into humus might be viewed as a removal of the elements of water from the carbon. The analysis of mouldered oak wood, which was taken from the interior of the trunk of an oak, and possessed a chocolate brown colour and the structure of wood, showed that 100 parts of it contained 53 - 36 parts of carbon and 46'44 parts of hydrogen and oxygen in the same relative proportions as in water. From an examination of mouldered wood of a light brown colour, easily reducible to a fine powder, and taken from another oak, it appeared that it con- tained 56-211 carbon and 43789 water. These indisputable facts point out the similarity of the decay of wood with the slow combustion or oxidation of bodies which contain a large quantity of hydrogen. Viewed as a kind of combustion, it would indeed be a very extraordinary process, if the carbon combined directly with the oxy- gen ; for it would be a combustion in which the carbon of the burning body augmented constantly, instead of diminishing. Hence it is evident that it is the hydrogen which is oxidised at the expense of the oxygen of the air ; while the carbonic acid is formed from the elements of the wood. Carbon never combines at common temperatures with oxygen, so as to form carbonic acid. In whatever stage of decay wood may be, its elements must always be capable of be- ing represented by their equivalent numbers. The following formula illustrates this fact with great clearness : C36 H22 O22 oak wood, according to Gay- Lussac and Thenard.* C35 H20 O20 humus from oak wood (Meyer.)t C34 HIS 18 humus from oak wood (Dr. Will.)* It is evident from these numbers that for every two equivalents of hydrogen which are oxidised, two atoms of oxygen and cne of carbon are set free. Under ordinary circumstances, woody fibre requires a very long time for its decay ; but this process is of course much accele- rated by an elevated temperature and free un- restrained access of air. The decay, on the contrary, is much retarded by absence of moisture, and by the wood being surrounded with an atmosphere of carbonic acid, which prevents the access of air to the decaying matters. Sulphurous acid, and all antiseptic sub- stances, arrest the decay of woody fibre. It * The calculation gives 52'5 carbon, and 47'5 water. t The calculation gives 54 carbon, and 46 water. t The calculation gives 56 carbon, and 44 water. is well known that corrosive sublimate is employed for the purpose of protecting the timber of ships from decay ; it is a substance which completely deprives vegetable or ani- mal matters, the most prone to decomposi- tion, of their property of entering into fer- mentation, putrefaction, or decay. But the decay of woody fibre is very much accelerated by contact with alkalies or alkaline earths; for these enable substances to absorb oxygen, which do not possess this power themselves ; alcohol, gallic acid, tannin, the vegetable colouring matters, and several other substances, are thus affected by them. Acids produce quite an opposite effect ; they greatly retard decay. Heavy soils, consisting of loam, retain longest the most important condition for the decay of the vegetable matter contained in them, viz., water; but their impermeable nature prevents contact with the air. In moist sandy soils, particularly such as are composed of a mixture of sand and car- bonate of lime, decay proceeds very quickly, it being aided by the presence of the slightly alkaline lime. Now let us consider the decay of woody fibre during a very long period of time, and suppose that its cause is the gradual removal f the hydrogen in the form of water, and the separation of its oxygen in that of car- bonic acid. It is evident that if we sub- tract from the formula C 86 , H 22 , O 22 , the 22 equivalents of oxygen, with 11 equivalents of carbon, and 22 equivalents of hydrogen, which are supposed to be oxidised by *he oxygen of the air, and separated in the form of water; then from 1 atom of oak wood, 25 atoms of pure carbon will remain as the final product of the decay. In other words, 100 parts of oak, which contain 52*5 parts of carbon, will leave as a residue 37 parts of carbon, which must remain unchanged, since carbon does not combine with oxygen at common temperatures. But this final result is never attained in the decay of wood under common circum- stances ; and for this reason, that with the increase of the proportion of carbon in the residual humus, as in all decompositions of this kind, its attraction for the hydrogen, which still remains in combination, also in- creases, until at length the affinity of oxygen for the hydrogen is equalled by that of the carbon for the same element. In proportion as the decay of woody fibre advances, its property of burning with flame, or in other words, of developing carburetted hydrogen on the application of heat, dimi- nishes. Decayed wood burns without flame; whence no other conclusion can be drawn, than that the hydrogen, which analysis shows to be present, is not contained in it in the same form as in wood. Decayed oak contains more carbon than fresh wood, but its hydrogen and oxygen are in the same proportion. We would naturally expect that the flame given out by decayed wood should be more 112 AGRICULTURAL CHEMISTRY. onlhant, in proportion to the increase of its carbon, but we find, on the contrary, that it burns like tinder, exactly as if no hydrogen were present. For the purposes of fuel, decayed or diseased wood is of little value, for it does not possess the property of burn- ing with flame, a property upon which the advantages of common wood depend. The hydrogen of decayed wood must conse- quentfy be supposed to be in the state of water; for had it any other form, the charac- ters we have described would not be pos- sessed by the decayed wood. If we suppose decay to proceed in a liquid, which contains both carbon and hydrogen, then a compound containing still more car- bon must be formed, in a manner similar to the production of the crystalline colourless naphthalin from a gaseous compound of carbon and hydrogen. And if the compound thus formed were itself to undergo further decay, the final result must be the separation of carbon in a crystalline form. Science can point to no process capable of accounting for the origin and formation of diamonds, except the process of decay. Diamonds cannot be produced by the action of fire, for a high temperature, and the pre- sence of oxygen gas, would call into play their combustibility. But there is the greatest reason to believe that they are formed in the humid way, that is, in a liquid, and the pro- cess of decay is the only cause to which their formation can with probability be ascribed. Amber, fossil resin, and the acids in mel- lite, are the products of vegetable matter, which has suffered decomposition. They are found in wood or brown coal, and have evidently proceeded from the decomposition of substances which were contained in quite a different form in the living plants-. They are all distinguished by the proportionally small quantity of hydrogen which they con- tain. The acid from mellite (mellitic acid) contains precisely the same proportions of carbon and oxygen as that from amber (suc- cinic acid;) they differ only in the propor- tion of their hydrogen. M. Bromeis* found that succinic acid might be artificially formed by the action of nitric acid on stearic acid, a true process of eremacausis; the experiment was made in this laboratory (Giessen.) CHAPTER XI. VEGETABLE MOULD. THE term vegetable mould, in its general signification, is applied to a mixture of dis- integrated minerals, with the remains of animal and vegetable substances. It may be considered as earth in which humus is contained in a state of decomposition. Its action upon the air has been fully investi- gated by Ingenhouss and De Saussure. When moist vegetable mould is placed in a vessel full of air, it extracts the oxygen * Liebig's Annaien, Band xxxiv., Heft 3. therefrom with greater rapidity than decayed wood, and replaces it by an equal volume of carbonic acid. When this carbonic acid is removed and fresh air admitted, the same action is repeated. Cold water dissolves only 10 ,Q 00 th of its own weight of vegetable mould; and the residue left on its evaporation consists of common salt with traces of sulphate of pot- ash and lime, and a minute quantity of or- ganic matter, for it is blackened when heated to redness. Boiling water extracts several substances from vegetable mould, and ac- quires a yellow or yellowish brown colour, which is dissipated by absorption of oxygen from the air, a black flocculent deposit being formed. When the coloured solution is evaporated, a residue is left which becomes black on being heated to redness, and after- wards yields carbonate of potash when treated with water. A solution of caustic potash becomes black when placed in contact with vegetable mould, and the addition of acetic acid to the coloured solution causes no precipitate or turbidity. But dilute sulphuric acid throws down a light flocculent precipitate of a brown or black colour, from which the acid can be removed with difficulty by means of water. When this precipitate, after having been washed with water, is brought whilst still moist under a receiver filled with oxy- gen, the gas is absorbed with great rapidity ; and the same thing takes place when the precipitate is dried in the air. In the per- fectly dry state it has entirely lost its solu- bility in water, and even alkalies dissolve only traces of it. It is evident, therefore, that boiling water extracts a matter from vegetable mould, which owes its solubility to the presence of the alkaline salts contained in the remains of plants. This substance is a product of the incomplete decay of woody fibre. Its composition is intermediate between woody fibre and humus, into which it is converted, by being exposed in a moist condition to the action of the air. CHAPTER XII. ON THE MOULDERING OF BODIES. PAPER, BROWN COAL, AND MINERAL COAL. THE decomposition of wood, woody fibre, and all vegetable bodies when subjected to the action of water, and excluded from the air, is termed mouldering. Wood, or brown coal and mineral coal, are the remains of vegetables of a former world; their appearance and characters show, that they are products of the pro- cesses of decomposition termed decay and putrefaction. We can easily ascertain by- analysis the manner in which their consti- tuents have been changed, if we suppose the greater part of their bulk to have been formed from woody fibre. But it is necessary, before we can obtain MOULDERING OF BODIES. 113 a distinct idea of the manner in which coal is formed, to consider a peculiar change which woody fibre suffers by means of moisture, when partially or entirely ex- cluded from the air. It is known, that when pure woody fibre, as linen, for example, is placed in contact with water, considerable heat is evolved, and the substance is converted into a soft friable mass which has lost all coherence. This substance was employed in the fabri- cation of paper before the use of chlorine, as an agent for bleaching. The rags employed for this purpose were placed in heaps, and it was observed, that on their becoming warm a gas was disengaged, and their weight diminished from 18 to 25 per cent. When sawdust moistened with water is placed in a closed vessel, carbonic acid gas is evolved in the same manner as when air is admitted. A true putrefaction takes place, the wood assumes a white colour, loses its peculiar texture, and is converted into a rot- ten friable matter. The white decayed wood found in the in- terior of trunks of dead trees which have been in contact with water, is produced in the way just mentioned. An analysis of wood of this kind, ob- tained from the interior of the trunk of an oak, yielded, after having been dried at 212, Carbon Hydrogen Oxygen Ashes 47-11 6-31 45-31 1-27 100-00 48-14 6-06 44-43 137 100-00 Now, on comparing the proportions ob- tained from these numbers with the compo- sition of oak wood, according to the analysis of Gay-Lussac and Thenard, it is imme- diately perceived, that a certain quantity of carbon has been separated from the consti- tuents of wood, whilst the hydrogen is, on the contrary, increased. The numbers ob- tained by the analysis correspond very nearly to the formula C33 H27 O24.* The elements of water have, therefore, become united with the wood, whilst car- bonic acid is disengaged by the absorption of a certain quantity of oxygen. If the elements of 5 atoms of water and 3 atoms of oxygen be added to the composi- tion of the woody fibre of the oak, and 3 atoms of carbonic acid deducted, the exact formula for white mouldered wood is ob- tained. Wood C36 H22 O22 To this add 5 atoms of water - H 5 O 5 3 atoms of oxygen - O 3 Subtract from this 3 atoms car- bonic asid C36 H27 030 C 3 6 C33 H27 024 * The calculation from this formula gives in 100 parta 47'9 carbon, 6'1 hydrogen, and 46 oxygen. The process of mouldering is, therefore one of putrefaction and decay, proceeding simultaneously, in which the oxygen of the air and the component parts of water take part. But the composition of mouldered wood must change according as the access of oxygen is more or less prevented. White mouldered beech-wood yielded on analysis 47'67 carbon, 5-67 hydrogen, and 46-(>8 oxygen; this corresponds to the formula C3"3 H25 024. The decomposition of wood assumes, therefore, two different forms, according as the access of the air is free or restrained. In both cases carbonic acid is generated; and in the latter case, a certain quantity of water enters into chemical combination. It is highly probable that in this putrefac- tive process, as well as in all others, the oxygen of the water assists in the formation of the carbonic acid. Wood coal (brown coal of Werner) must have been produced by a process of decom- position similar to that of mouldering. But it is not easy to obtain wood coal suited for analysis, for it is generally impregnated with resinous or earthy substances, by which the composition of those parts which have been formed from woody fibre is essentially changed. The wood coal, which forms extensive layers in the Wetterau (a district in Hesse Darmstadt,) is distinguished from that found in other places, by possessing the structure of wood unchanged, and by containing ao bituminous matter. This coal was subjec'ed to analysis, a piece being selected upon which the annual circle could be counted. It was obtained from the vicinity of Lau- bach; 100 parts contained Carbon .... 57'28 Hydrogen - - - - 6 '03 Oxygen ..... SG'10 Ashes .... Q-59 100-00 The large amount of carbon, and small quantity of oxygen, constitute the most ob- vious difference between this analysis and that of wood. It is evident that tae wood which has undergone the change into coal must have parted with a certain portion of its oxygen. The proportions of these num- bers are expressed by the formula C33 H21 016.* When these numbers are compared with those obtained by the analysis of oak, it would appear that the brown coal was pro- duced from woody fibre by the separation of one equivalent of hydrogen, and the ele- ments of three equivalents of carbonic acid. 1 atom wood C36 H22 O22 Minus 1 atom hydrogen and 37 r Q , ^ c atoms carbonic actf - C 3 H 1 C Wood coal, C33 H21 O16 * The calculation gives 57'5 carbon, and 5'98 hydrogen. 114 AGRICULTURAL CHEMISTRY. All varieties of wood coal, from whatever strata they may be taken, contain more hy- drogen than wood does, and less oxygen than is necessary to form water with this hydrogen; consequently they must all be produced by the same process of decompo- sition. The excess of hydrogen is either hydrogen of the wood which has remained in it unchanged, or it is derived from some exterior source. The analysis of wood coal from Ringkuhl, near Cassel, where it is seldom found in pieces with the structure of wood, gave, when dried at 212, Carbon Hydrogen Oxygen Ashes 62-60 5-02 26-52 5-86 100-00 6383 4-80 25-51 5-86 100-00 The proportions derived from these num- bers correspond very closely to the formula Q32 JJ 15 O 9 , or they represent the constitu- ents of wood, from which the elements of carbonic acid, water, and 2 equivalents hy- drogen, have been separated. C36H22 022+ Wood. Subtract C 4 H 7 O13-f-4 atoms carbonic acid-f- 5 atoms of water 2 atoms of hydrogen. C32 H15 9= Wood Coal from Ring- kuhl. The formation of both these specimens of wood coal appears from these formulae to have taken place under circumstances which did not entirely exclude the action of the air, and consequent oxidation and removal of a certain quantity of hydrogen. Now the Laubacher coal is covered with a layer of basalt, and the coal of Ringkuhl was taken from the lowest seam of layers, which pos- sess a thickness of from 90 to 120 feet; so that both may be considered as well protected from the air. During the formation of brown coal, the elements of carbonic acid have been sepa- rated from the wood either alone, or at the same time with a certain quantity of water. It is quite possible that the difference in the process of decomposition may depend upon the high temperature and pressure under which the decomposition took place. At least, a piece of wood assumed the character and appearace of Laubacher coal, after be- ing kept for several weeks in the boiler of a steam engine, and had then precisely the same composition. The change in this case was effected in water, at a temperature of from 3340 to 352 F. (150 160 C.,) and under a corresponding pressure. The ashes of the wood amounted to 0'51 per cent. ; a little less, therefore, than those of the Lau- bacher coal ; but this must be ascribed to the peculiar circumstances under which it was formed. The ashes of plants examined by Bertnier amounted always to much more than this. The peculiar process by which the de- composition of these extinct vegetables has >een effected, namely, a disengagement of carbonic acid from their substance, appears still to go on at great depths in all the layers of wood coal. At all events it is remarkable that springs impregnated with carbonic acid >ccur in many places, in the country be* ween Meissner, in the electorate of Hesse, and the Eifel, which are known to possess arge layers of wood coal. These springs of mineral water are produced on the spot at which they are found ; the springs of common water meeting with carbonic acid during their ascent, and becoming impreg- nated with it. In the vicinity of the layers of wood coal at Salshausen (Hesse Darmstadt) an excel- lent acidulous spring of this kind existed a few years ago, and supplied all the inhabi- tants of that district; but it was considered advantageous to surround the sides of the spring with sandstone, and the consequence was, that all the outlets to the carbonic acid were closed, for this gas generally gains ac- cess to the water from the sides of the spring. From that time to the present this valuable mineral water has disappeared, and in its place is found a spring of common water. Springs of water impregnated with car- bonic acid occur at Schwalheim, at a very short distance from the layers of wood coal at Dorheim. M. Wilhelmi observed some time since, that they are formed of common spring water which ascends from below, and of carbonic acid which issues from the sides of the spring. This same fact has been shown to be the case in the famed Fachinger spring, by M. Schapper. The carbonic acid gas from the springs m the Eifel is, according to Bischoff, seldom mixed with nitrogen or oxygen, and is pro- bably produced in a manner similar to that just described. At any rate the air does not appear to take any part in the formation of these acidulous springs. The carbonic acid has evidently not been formed either by a combustion at high or low temperatures j for if it were so, the gas resulting from the combustion would necessarily be mixed with of nitrogen, but it does not contain a trace of this element. The bubbles of gas which escape from these springs are absorbed by caustic potash, with the exception of a resi- duum too small to be appreciated. The wood coal of Dorheim and Salzhau- sen must have been formed in the same way as that of the neighbouring village of Lau- bach ; and since the latter contains the exact elements of woody fibre, minus a certain quantity of carbonic acid, its composition indicates very plainly the manner in which it has been produced. The coal of the upper bed is subjected to an incessant decay by the action of the air, j by means of which its hydrogen is removed | in the same manner as in the decay of wood. This is recognised by the way in which it burns, and by the formation of carbonic acid in the mines. POISONS, CONTAGIONS, MIASMS. 115 The gases which are formed in mines of wood coal, and cause danger in their work- ing, are not combustible or inflammable as in mines of mineral coal ; but they consist generally of carbonic acid gas, and are very seldom intermixed with combustible gases. Wood coal from the middle bed of the strata at Ringkuhl gave on analysis 65*40 64'01 carbon and 4*75 4*76* hydrogen ; the proportion of carbon here is the same as in specimens procured from greater depths, but that of the hydrogen is much less. Wood and mineral coal are always ac- companied by iron pyrites (sulphuret of iron) or zinc blende (sulphuret of zinc ;) which minerals are still formed from salts of sulphuric acid, with iron or zinc, during the putrefaction of all vegetable matter. It is possible that the oxygen of the sulphates in the layers of wood coal is the means by which the removal of the hydrogen is effected, since wood coal contains less of this element than wood. According to the analysis of Richardson and Regnault, the composition of the com- bustible materials in splint coal from New- castle, and cannel coal from Lancashire, is expressed by the formula C24 HI 3 O. When this is compared with the composition of woody fibre, it appears that these coals are formed from its elements, by the re- moval of a certain quantity of carburetted hydrogen and carbonic acid in the form of combustible oils. The composition of both of these coals is obtained by the subtraction of 3 atoms of carburetted hydrogen, 3 atoms of water, and 9 atoms of carbonic acid from the formula of wood. 3 atoms of carburet- ted hydrogen C3 3 atoms of water H3 9 atoms of carbonic acid - - C9 O18 C12H9021 Hfi 03 C36 H22 022 =wood Mineral coal|C24 H13 O Carburetted hydrogen generally accom- panies all mineral coal; other varieties of coal contain volatile oils which may be sepa- rated by distillation with water. (Reichen- bach.) The origin of naphtha is owing to a similar process of decomposition. Caking coal from Caresfield, near Newcastle, con- tains the elements of cannel coal, minus the constituents of defiant gas C4 H4. The inflammable gases which stream out of clefts in the strata of mineral coal, or in rocks of the coal formations, always con- tain carbonic acid, according to a recent examination by Bischoff, and also carburet- ted hydrogen, nitrogen, and olefiant gas; the last of which had not been observed, until its existence in these gases was pointed out by Bischoff. The analysis of fire-damp * The analysis of brown coal from Ringkuhl, as well as all those of the same substance given in this work, have been executed in this labora- tory by M. Kiihnert of Cassel. after it had been treated with caustic potash showed its constituents to be, Gas from an abandoned Gerhard's Gas from a mine near passage near mine near Wallesvveiler. Luisenthal. Lkkwtge. Vol. Vol. Vol. Light carburetted hydrogen 91'36 83.08 79'10 Olefiant gas 6'32 1'98 IG'll Nitrogen gas 2'32 14'94 4'79 100-00 100-00 100-00 The evolution of these gases proves that changes are constantly proceeding in the coal. It is obvious from this, that a continual removal of oxygen in the form of carbonic acid is effected from layers of wood coal, in consequence of which the wood must ap- proach gradually to the composition of mineral coal. Hydrogen, on the contrary, is disengaged from the constituents of mineral coal in the form of a compound of carbo-hy- drogen ; a complete removal of all the hydro- gen would convert coal into anthracite. The formula C36 H22 O22, which is given for wood, has been chosen as the em- pirical expression of the analysis, for the purpose of bringing all the transformations which woody fibre is capable of undergoing under one common point of view. Now, although the correctness of this formula must be doubted, until we know with certainty the true constitution of woody fibre, this cannot have the smallest influence on the account given of the changes to which woody fibre must necessarily be subjected in order to be converted into wood or mineral coal. The theoretical expression refers to the quantity, the empirical merely to the relative proportion in which the elements of a body are united. Whatever form the first may assume, the empirical expression must always remain unchanged. CHAPTER XIII. ON POISONS, CONTAGIONS, AND MIASMS. A GREAT many chemical compounds, some derived from inorganic nature, and others formed in animals and plants, pro- duce peculiar changes or diseases in the living animal organism. They destroy the vital functions of individual organs; and when their action attains a certain degree of intensity, death is the consequence. The action of inorganic compounds, such as acids, alkalies, metallic oxides, and salts, can in most cases be easily explained. They either destroy the continuity of particular organs, or they enter into combination with their substance. The action of sulphuric, muriatic, and oxalic acids, hydrate of pot- ash, and all those substances which produce the direct destruction of the organs with which they come into contact, may be com- pared to a piece of iron, which can causa 116 AGRICULTURAL CHEMISTRY. death by inflicting an injury on particular] During the passage of these salts through organs, either when heated to redness, or the lungs, their acids take part in the pecu- when in the form of a sharp knife. Such liar process of eremacausis which proceeds substances are not poisons in the limited sense of the word, for their injurious action depends merely upon their condition. The action of the proper inorganic poisons is owing, in most cases, to the formation of a chemical compound by the union of the poison with the constituents of the organ upon which it acts; it is owing to an exer- cise of a chemical affinity more powerful than the vitality of the organ. It is well to consider the action of inor- ganic substances in general, in order to ob- tain a clear conception of the mode of action of those which are poisonous. We find that certain soluble compounds, when pre- sented to different parts of the body, are ab- sorbed by the blood, whence they are again eliminated by the organs of secretion, either in a changed or in an unchanged state. Iodide of potassium, sulpho-cyanuret of potassium, ferro-cyanuret of potassium, chlorate of potash, silicate of potash, and all salts with alkaline bases, when administered internally to man and animals in dilute solu- tions, or applied externally, may be again detected in the blood, sweat, chyle, gall, and splenic veins ; but all of them are finally ex- creted from the body through the urinary passages. Each of these substances, in its transit, produces a peculiar disturbance in the or- ganism in other words, they exercise a medicinal action upon it, but they them- selves suffer no decomposition. If any of these substances enter into combination with any part of the body, the union cannot be of a permanent kind; for their reappearance in the urine shows that any compounds thus formed must have been again decom- posed by the vital processes. Neutral citrates, acetates, and tartrates of the alkalies, suffer change in their passage through the organism. Their bases can indeed be detected in the urine, but the acids have entirely disappeared, and are replaced by carbonic acid which has united with the bases. (C4ilbert Blane and Wohler.) The conversion of these salts of organic acids into carbonates, indicates that a con- siderable qantity of oxygen must have united with their elements. In order to convert 1 equivalent of acetate of potash into the car- bonate of the same base, 8 equivalents of oxygen must combine with it, of which either 2 or 4 equivalents (according as an acid or neutral salt is produced) remain in combination with the alkali; whilst the re- maining 6 or 4 equivalents are disengaged as free carbonic acid. There is no evidence presented by the organism itself, to which these salts have been administered, that any of its proper constituents have yielded so great a quantity of oxygen as is necessary for their conversion into carbonates. Their oxidation can, therefore, only be ascribed to the oxygen of the air. in that organ ; a certain quantity of the oxy- gen gas inspired unites with their constitu- ents., and converts their hydrogen into water, and their carbon into carbonic acid. Part of this latter product (1 or 2 equivalents) remains in combination with the alkaline base, forming a salt which suffers no farther change by the process of oxidation; and it is this salt which is separated by the kidneys or liver. It is manifest that the presence of these organic salts in the blood must produce a change in the process of respiration. A part of the oxygen inspired, which usually com- bines with the constituents of the blood, must, when they are present, combine with their acids, and thus be prevented from per- forming its usual office. The immediate consequence of this must be the formation of arterial blood in less quantity, or in other words, the process of respiration must be retarded. Neutral acetates, tartrates, and citrates placed in contact with the air, and at the same time with animal or vegetable bodies in a state of eremacausis, produce exactly the same effects as we have described them to produce in the lungs. They participate in the process of decay, and are converted into carbonates just as in the living body. If impure solutions of these salts in water are left exposed to the air for any length of time, their acids are gradually decomposed, and at length entirely disappear. Free mineral acids, or organic acids which are not volatile, and salts of mineral acids with alkaline bases, completely arrest decay when added to decaying matter in sufficient quantity ; and when their quantity is small, the process of decay is protracted and re- tarded. They produce in living bodies the same phenomena as the neutral organic salts, but their action depends upon a differ- ent cause. The absorption by the blood of a quantity of an inorganic salt sufficient to arrest the process of eremacausis in the lungs, is pre- vented by a very remarkable property of all animal membranes, skin, cellular tissue, muscular fibre, &c. ; namely, by their inca- pability of being permeated by concentrated saline solutions. It is only when these so- lutions are diluted to a certain degree with water that they are absorbed by animal tissues. A dry bladder remains more or less dry in saturated solutions of common salt, nitre, ferro-cyanuret of potassium, sulpho-cyanu- ret of potassium, sulphate of magnesia, chloride of potassium, and sulphate of soda. These solutions run off its surface in the same manner as water runs from a plate of glass besmeared with tallow. Fresh flesh, over which salt has been strewed, is found after 24 hours' swimming in brine, although not a drop of water has POISONS, CONTAGIONS, MIASMS. 117 been added. The water has been yielded by muscular fibre itself, and having dis- solved the salt in immediate contact with it, and thereby lost the power of penetrating animal substances,, it has on this account separated from the flesh. The water still retained by the flesh contains a proportion- ally small quantity of salt, having that de- gree of dilution at which a saline fluid is capable of penetrating animal substances. This property of animal tissues is taken advantage of in domestic economy for the purpose of removing so much water from meat that a sufficient quantity is not left to enable it to enter into putrefaction. In respect of this physical property of animal tissues, alcohol resembles the inor- ganic salts. It is incapable of moistening, that is, of penetrating, animal tissues, and possesses such an affinity for water as to extract it from moist substances. When a solution of a salt, in a certain de- gree of dilution, is introduced into the sto- mach, it is absorbed ; but a concentrated saline solution, in place of being itself ab- sorbed, extracts water from the organ, and a violent thirst ensues. Some interchange of water and salt takes place in the stomach ; the coats of this viscus yield water to the solution, a part of which having previously become sufficiently diluted, is, on the other hand, absorbed. But the greater part of the concentrated solution of salt remains unab- sorbed, and is not removed by the urinary passages; it consequently enters the intes- tines and intestinal canal, where it causes a dilution of the solid substances deposited there, and thus acts as a purgative. Each of the salts just mentioned pos- sesses this purgative action, which depends on a physical property shared by all of them ; but besides this they exercise a me- dicinal action, because every part of the organism with which they come in contact absorbs a certain quantity of them. The composition of the salts has nothing to do with their purgative action ; it is quite ; a matter of indifference as far as the mere ! production of this action is concerned (not as to its intensity,) whether the base be potash or soda, or in many cases lime and j magnesia ; and whether the acid be phos- | phoric, sulphuric, nitric, or hydrochloric. Besides these salts, the action of which ; does not depend upon their power of enter- ! ing into combination with the component { parts of the organism, there is a large class of others which, when introduced into the living body, effect changes of a very differ- ent kind, and produce diseases or death, ac- cording to the nature of these changes, with- out effecting a visible lesion of any organs. These are the true inorganic poisons, the action of which depends upon their power of forming permanent compounds with the substance of the membranes, and muscular fibre. Salts of lead, iron, bismuth, copper, and mercury, belong to this class. When solutions of these salts are treated with a sufficient quantity of albumen, milk, muscular fibre, and animal membranes, they enter into combination with those sub- stances, and lose their own solubility ; while the water in which they were dissolved loses all the salt which it contained. The salts of alkaline bases extract water from animal substances ; whilst the salts of the heavy metallic oxides are, on the con- trary, extracted from the water, for they enter into combination with the animal matters. Now, when these substances are adminis- tered to an animal, they lose their solubility by entering into combination with the mem- branes, cellular tissue, and muscular fibre; but in very few cases can they reach the blood. All experiments instituted for the purpose of determining whether they pass into the urine have failed to detect them in that secretion. In fact, during their pas- sage through the organism, they come into contact with many substances by which they are retained. The action of corrosive sublimate and arsenious acid is very remarkable in this respect. It is known that these substances possess, in an eminent degree, the property of entering into combination with all parts of animal and vegetable bodies, rendering them at the same time insusceptible of decay or putrefaction. Wood and cerebral sub- stance are both bodies whicn undergo change with great rapidity and facility when sub- ject to the influence of air and water ; but if they are digested for some time with ar- senious acid or corrosive sublimate, they may subsequently be exposed to all the in- fluences of the atmosphere without altering in colour or appearance. It is farther known that those parts of a body which come in contact with these sub- stances during poisoning, and which there- fore enter into combination with them, do not afterwards putrefy ; so that there can be no doubt regarding the cause of their poi- sonous qualities. It is obvious that if arsenious acid and corrosive sublimate are not prevented by the vital principle from entering into combina- tion with the component parts of the body, and consequently from rendering them inca- pable of decay and putrefaction, they must deprive the organs of the principal property which appertains to their vital condition, viz. that of suffering and effecting trans- formations ; or, in other words, organic life must be destroyed. If the poisoning is merely superficial, and the quantity of the poison so small that only individual parts of the body which are capable of being re- generated have entered into combination with it, then eschars are produced a phe^ nomonon of a secondary kind the com- pounds of the dead tissues with the poison being thrown off by the healthy parts. From these considerations it may readily be inferred that all internal signs of poisoning 118 AGRICULTURAL CHEMISTRY. are variable and uncertain ; for cases may happen, in which no apparent indication of change can be detected by simple observa- tions of the parts, because, as has been- al- ready remarked, death may occur without the destruction of any organs. When arstnious acid is administered in solution, it may enter into the blood. If a vein is exposed and surrounded with a solu- tion of this acid, every blood-globule will combine with it, that is, will become poi- soned. The compounds of arsenic, which have not the property of entering into combina- tion with the tissues of the organism, are without influence on life, even in large doses. Many insoluble basic salts of arsenious acid are known not to be poisonous. The sub- stance called alkargen, discovered by Bunsen, has not the slightest injurious action upon the organism ; yet it contains a very large quantity of arsenic, and approaches very closely in composition to the organic arse- nious compounds found in the body. These considerations enable us to fix with tolerable certainty the limit at which the above substances cease to act as poisons. For since their combination with organic matters must be regulated by chemical laws, death will inevitably result, when the organ in contact Avith the poison finds sufficient of it to unite with atom for atom ; whilst if the poison is present in smaller quantity, a part of the organ will retain its vital func- tions. According to the experiments of Mulder,* the equivalent in which fibrin combines with muriatic acid, and with the oxides of lead and copper, is expressed by the number 6361 . It may be assumed therefore approxima- tely, that a quantity of fibrin correspond- ing to the number 6361 combines with 1 equivalent of arsenious acid, or 1 equiva- lent of corrosive sublimate. When 6361 parts of anhydrous fibrin are combined with 30,000 parts of water, it is in the state in which it is contained in mus- cular fibre or blood in the human body. 100 , /tins of fibrin in this condition would form a neutral compound of equal equiva- lents with 3 1% grains of arsenious acid, and 5 grains of corrosive sublimate. The atomic weight of the albumen of eggs and of the blood deduced from the analysis of the compound which it forms with oxide of silver is 7447, and that of animal gelatin 5652. 100 grains of albumen containing all the water with which it is combined in the liv- ing body, should consequently combine with \i grain of arsenious acid. These proportions which may be consi- dered as the highest which can be adopted, indicate the remarkably high atomic weights of animal substances, and at the same lime teach us what very small quantities of arse- * Poggendorff's Annalen, Band xl. S. 259. nious acid or corrosive sublimate are requi- site to produce deadly effects. All substances administered as antidotes in cases of poisoning, act by destroying the power which arsenious acid and corrosive sublimate possess, of entering into combi- nation with animal matters, and of thus acting as poisons. Unfortunately no other body surpasses them in that power, and the compounds which they form can only be broken up by affinities so energetic, that their action is as injurious as that of the above-named poisons themselves. The duty of the physician consists, therefore, in his causing those parts of the poison which may be free and still uncombined, to enter into combination with some other body, so as to produce a compound incapable of being decomposed or digested in the same conditions. Hydrated peroxide of iron is an invaluable substance for this purpose. When the action of arsenious acid or corrosive sublimate is confined to the, sur- face of an organ, those parts only are de- stroyed which enter into combination with it; an eschar is formed, which is gradually thrown off. Soluble salts of silver would be quite as deadly a poison as corrosive sublimate, did not a cause exist in the human body by which their action is prevented, unless their quantity is very great. This cause is the presence of common salt in all animal liquids. Nitrate of silver, it is well known, combines with animal substances, in the same manner as corrosive sublimate, and the compounds formed by both are exactly similar in the character of being incapable of decay or putrefaction. When nitrate of silver in a state of solu- tion is applied to skin or muscular fibre, it combines with them instantaneously ; ani- mal substances dissolved in any liquid are precipitated by it, and rendered insoluble, or, as it is usually termed, they are coagu- lated. The compounds thus formed are colourless, and so stable, that they cannot be decomposed by other powerful chemical agents. They are blackened by exposure to light, like all other compounds of silver, in consequence of a part of the oxide of silver which they contain being reduced to the metallic state. Parts of the body which have united with salts of silver no longer belong to the living organism, for their vital functions have been arrested by combina- tion with oxide of silver ; and if they are capable of being reproduced, the neighbour- ing living structures throw them off in the form of an eschar. When nitrate of silver is introduced into the stomach, it meets with common salt and free muriatic acid ; and if its quantity is not too great, it is immediately converted into chloride of silver a substance which is absolutely insoluble in pure water. In a solution of salt or muriatic acid, however, chloride of silver does dissolve in extremely minute quantity j and it is this small part POISONS, CONTAGIONS, MIASMS. 119 which exercises a medicinal influence when nitrate of silver is administered; the remain- ing chloride of silver is eliminated from the body in the ordinary way. Soluhility is necessary to give efficacy to any substance in the human body. The soluble salts of lead possess many properties in common with the salts of silver and mercury ; but all compounds of lead with organic matters are capable of decom- position by dilute sulphuric acid. The dis- ease called painter's colic is unknown in all manufactories of while lead in which the workmen are accustomed to take as a pre- servative sulphuric acid lemonade (a solu- tion of sugar rendered acid by sulphuric acid.) The organic substances which have com- bined in the living body with metallic oxides or metallic salts, lose their property of im- bibing water and retaining it, without at the same time being rendered incapable of per- mitting liquids to penetrate through their pores. A strong contraction and shrinking of the surface is the general effect of contact with these metallic bodies. But corrosive sublimate, and several of the salts of lead, possess a peculiar property, in addition to those already mentioned. When they are present in excess, they dissolve the first formed insoluble compounds, and thus pro- duce an effect quite the reverse of contrac- tion, namely, a softening of the part of the body on which they have acted. Salts of oxide of copper, even when in combination with the most powerful acids, are reduced by many vegetable substances, particularly such as sugar and honey, either into metallic copper, or into the red sub- oxide, neither of which enters into combina- tion with animal matter. It is well known that sugar has been long employed as the most convenient antidote for poisoning b*y copper. With respect to some other poisons, namely, hydrocyanic acid and the organic bases strychnia and brucia, we are ac- quainted with no facts calculated to eluci- date the nature of their action. It may, however, be presumed with much certainty, that experiments upon their mode of action on differen* animal substances would very quickly lead to the most satisfactory conclu- sions regarding the cause of their poisonous effects. There is a peculiar class of substances, which are generated during certain pro- cesses of decomposition, and which act upon the animal economy as deadly poisons, not on account of their power of entering into combination with it, or by reason of their containing a poisonous material, but solely by virtue of their peculiar condition. In order to attain to a clear conception of the mode of action of these bodies, it is ne- cessary to call to mind the cause on which we have shown the phenomena of fermen- tation, decay, and putrefaction to depend. This cause may be expressed by the fol- lowing law, long since proposed by La Place and Berthollet, although its truth with re- spect to chemical phenomena has only lately been proved. "-J1 molecule set in motion by any power can impart its own motion to another molecule with which it may be in contact." This is a law of dynamics, the operation of which is manifest in all cases, in which the resistance (force, affinity, or cohesion*,) opposed to the motion is not sufficient to overcome it. We have seen that ferment or yeast is a body in the state of decomposition, the atoms of which, consequently, are in a state of motion or transposition. Yeast placed in contact with sugar communicates to the elements of that compound the same state, in consequence of which, the constituents of the sugar arrange themselves into new and simpler forms, namely, into alcohol and carbonic acid. In these new compounds the elements are united together by stronger affinities than they were in the sugar, and therefore under the conditions in which they were produced further decomposition is arrested. We know, also, that the elements of sugar assume totally different arrangements, when the substances which excite their transposition are in a different stale of de- composition from the yeast just mentioned. Thus, when sugar is acted on by rennet or putrefying vegetable juices, it is not con- verted into alcohol and carbonic acid, but into lactic acid, mannite, and gum. Again, it has been shown, that yeast added to a solution of pure sugar gradually disappears, but that when added to vege- table juices which contain gluten as well as sugar, it is reproduced by the decomposition of the former substance. The yeast with which these liquids are made to ferment has itself been originally produced from gluten. The conversion of gluten into yeast in these vegetable juices is dependent on the decomposition (fermentation) of sugar; for, when the sugar has completely disappeared, any gluten which may still remain in the liquid does not suffer change from contact with the newly-deposited yeast, but retains all the characters of gluten. Yeast is a product of the decomposition of gluten ; but it passes into a second stage of decomposition when in contact with water. On account of its being in this state of further change, yeast excites fermen- tation in a fresh solution of sugar, and if this second saccharine fluid should contain gluten, (should it be wort, for example,) yeast is again generated in consequence of the transposition of the elements of the sugar exciting a similar change in this gluten. After this explanation, the idea that yeast reproduces itself as seeds reproduce seeds, cannot for a moment be entertained. From the foregoing facts it follows, that 120 AGRICULTURAL CHEMISTRY. a body in the act of decomposition (it may be named the exciter,") added to a mixed fluid in which its constituents are contained, can reproduce itself in that fluid, exactly in the same manner as new yeast is produced when yeast is added to liquids containing gluten. This must be more certainly ef- fected when the liquid acted upon contains the body by the metamorphosis of which the exciter has been originally formed. It is also obvious, that if the exciter be able to impart its own state of transformation to one only of the component parts of the mixed liquid acted upon, its own reproduc- tion may be the consequence of the decom- position of this one body. This law may be applied to organic sub- stances forming part of the animal organism. We know that all the constituents of these substances are formed from the blood, and that the blood by its nature and constitution is one of the most complex of all existing matters. Nature has adapted the blood for the re- production of every individual part of the organism ; its principal character consists in its component parts being subordinate to every attraction. These are in a perpetual state of change or transformation, which is effected in the most various ways through the influence of the different organs. The indivdual organs., such as the stomach, cause all the organic substances conveyed to them which are capable of transformation to assume new forms. The stomach com- pels the elements of these substances to unite into a compound fitted for the form- ation of the blood. But the blood pos- sesses no power of causing transformations ; on the contrary, its principal character con- sists in its readily suffering transformations ; and no other matter can be compared in this respect with it* Now it is a well-known fact, that when blood, cerebral substance, gall, pus, and other substances in a state of putrefaction, are laid upon fresh wounds, vomiting, de- bility, and at length death, are occasioned. It is also well known that bodies in anato- mical rooms frequently pass into a state of decomposition which is capable of imparting itself to the living body, the smallest cut with a knife which has been used in their dissection producing in these cases dan- gerous consequences. The poison of bad sausages belongs to this class of noxious substances. Several hun- dred cases are known in which death has occurred from the use of this kind of food. In Wurtemberg especially these cases are very frequent, for there the sausages are pre- pared from very various materials. Blood, liver, bacon, brains, milk, meal, and bread, are mixed together with salt and spices ; the mixture is then put into bladders or in- testines, and after being boiled is smoked. When these sausages are well prepared, they may be preserved for months, and fur- nish a nourishing savoury food ; but when ! the spices and salt are deficient, and particu- larly when they are smoked too late or not sufficiently, they undergo a peculiar kind ot putrefaction, which begins at the centre of the sausage. Without any appreciable escape of gas taking place they become paler in colour, and more soft and greasy in those parts which have undergone putre- faction, and they are found to contain free lactic acid, or lactate of ammonia ; products which are universally formed during the putrefaction of animal and vegetable mat- ters. The cause of the poisonous nature of these sausages was ascribed at first to hy- drocyanic acid, and afterwards to sebacic acid, although neither of these substances had been detected in them. But sebacic acid is no more poisonous than benzoic acid, with which it has so many properties in common ; and the symptoms produced are sufficient to show that hydrocyanic acid is not the poison. The death which is the consequence of poisoning by putrefied sausages succeeds very lingering and remarkable symptoms. There is a gradual wasting of muscular fibre, and of all the constituents of the body similarly composed; the patient becomes much emaciated, dries to a complete mum- my, and finally dies. The carcase is stiff as if frozen, and is not subject to putrefaction. During the progress of the disease the saliva becomes viscous and acquires an offensive smell. Experiments have been made for the pur- pose of ascertaining the presence of some matter in the sausages to which their poi- sonous action could be ascribed; but no such matter has been detected. Boiling water and alcohol completely destroy the poison- ous properties of the sausages, without themselves acquiring similar properties. Now this is the peculiar character of all substances which exert an action by virtue of their existing condition of those bodies the elements of which are in the state of de- composition or transposition ; a state which is destroyed by boiling water and alcohol without the cause of the influence being im- parted to those liquids ; for a state of action or power cannot be preserved in a liquid. Sausages, in the state here described, ex- ercise an action upon the organism, in con- sequence of the stomach and other parts with which they come in contact not having the power to arrest their decomposition ; and entering the blood in some way or other, while still possessing their whole power, they impart their peculiar action to the con- stituents of that fluid. The poisonous properties of decayed sau- sages are not destroyed by the stomach as those of the small-pox virus are. All the substances in the body capable of putrefac- tion are gradually decomposed during the course of the disease, and after death nothing remains except fat, tendons, bones, and a few other substances which are incapable of POISONS, CONTAGIONS, MIASMS. 121 putrefying in the conditions afforded by the It is impossible to mistake the modus ope- randi of this poison, for Colin has clearly proved that muscle, urine, cheese, cerebral substance, and other matters, in a state of putrefaction, communicate their own state of decomposition to substances much less prone to change of composition than the blood. When placed in contact with a so- lution of sugar, they cause its putrefaction, or the transposition of its elements into car- bonic acid and alcohol. When putrefying muscle or pus is placed upon a fresh wound, it occasions disease and death. It is obvious that these sub- stances communicate their own state of pu- trefaction to the sound blood from which they were produced, exactly in the same manner as gluten in a state ot decay or putrefaction causes a similar- transformation in a solution of sugar. Poisons of this kind are even generated by the body itself in particular diseases. In small-pox, plague, and syphilis, substances a peculiar nature are formed from the constituents of the blood. These matters are capable of inducing in the blood of a healthy individual a decomposition similar to that of which they themselves are the subjects ; in other words, they produce the same disease. The morbid virus appears to reproduce itself just as seeds appear to re- produce seeds. The mode of action of a morbid virus ex- hibits such a strong similarity to the action of yeast upon liquids containing sugar and gluten, that the two processes have been long since compared to one another, al- though merely for the purpose of illustra- tion. But when the phenomena attending the action of each respectively are con- sidered more closely, it will in reality be seen that their influence depends upon the same cause. In dry air, and in the absence of mois- ture, all these poisons remain for a long time unchanged ; but when exposed to the air in the moist condition, they lose very rapidly their peculiar properties. In the former case, those conditions are afforded which ar- rest their decomposition without destroying it ; in the latter, all the circumstances neces- sary for the completion of their decomposi- tion are presented. The temperature at which water boils, and contact with alcohol, render such poi- sons inert. Acids, salts of mercury, sul- phurous acid, chlorine, iodine, bromine, aromatic substances, volatile oils, and parti- cularly empyreumatic oils, smoke, and a decoction of coffee, completely destroy their contagious properties, in some cases com- bining with them or othenvise effecting their decomposition. Now all these agents, without exception, retard fermentation, pu- trefaction, and decay, and when present in sufficient quantity, completely arrest these processes of decomposition. 16 A peculiar matter, to which the poisonous action is due, cannot, we have seen, be ex- tracted from decayed sausages : and it is equally impossible to obtain such a principle from the virus of small-pox or plague, and for this reason, that their peculiar power is due to an active condition recognisable by our senses, only through the phenomena which it produces. In order to explain the effects of conta- gious matters, a peculiar principle of life has been ascribed to them a life similar to that possessed by the germ of a seed, which enables it under favourable conditions to de- velope and multiply itself. It would be im- possible to find a more correct figurative representation of these phenomena ; it is one which is applicable to contagions, as weil as to ferment, to animal and vegetable sub- stances in a state of fermentation, putrefac- tion or decay, and even to a piece of decay- ing wood, which by mere contact with fresh wood, causes the latter to undergo gradually the same change and become decayed and mouldered. If the property possessed by a body of producing such a change in any other sub- stance as causes the reproduction of itself, with all its properties, be regarded as life, then, indeed, all the above phenomena may be ascribed to life. But in that case they must not be considered as the only processes due to vitality, for the above interpretation of the expression embraces the majority of the phenomena which occur in organic che- mistry. Life would, according to that view, [/ be admitted to exist in every Ixxiy in which chemical forces act. If a body A, for example oxamide, (a sub- stance scarcely soluble in water, and without the slightest taste,) be brought into contact with another compound B, which is to be reproduced ; and if this second body be oxalic acid dissolved in water; then the following changes are observed to take place : The oxamide is decomposed by the oxalic acid, provided the conditions necessary for their exercising an action upon one another are present. The elements of water unite with the constituents of oxamide, and ammonia is one product formed, and oxalic acid the other, both in exactly the proper proportions to combine and form a neutral salt. Here the contact of oxamide and oxalic acid induces a transformation of the oxa- mide, which is decomposed into oxalic acid and ammonia. The oxalic acid thus formed, as well as that originally added, are shared by the ammonia or in other words, as much free oxalic acid exists after the de- composition as before it, and is of course still possessed of its original power. It mat- ters not whether the free oxalic acid is that originally added, or that newly produced; it is certain that it has been reproduced in an equal quantity by the decomposition. If we now add to the same mixture a fresh portion of oxamide, exactly equal in quan- tity to that first used, and treat it in the same 122 AGRICULTURAL CHEMISTRY. manner, the same decomposition is repeated; the free oxalic acid enters into combination, whilst another portion is liberated. In this manner a very minute quantity of oxalic acid may be made to effect the decomposi- tion of several hundred pounds of oxamide; and one grain of the acid to reproduce itself in unlimited quantity. We know that the contact of the virus of 8mall-pox causes such a change in the blood, as gives rise to the reproduction of the poi- son from the constituents of the fluid. This transformation is not arrested until all the particles of the blood which are susceptible of the decomposition have undergone the metamorphosis. We have just seen that the contact of oxalic acid with oxamide caused the production of fresh oxalic acid, which in its turn exercised the same action en a new portion of oxamide. The trans- formation was only arrested in consequence of the quantity of oxamide present being limited. In their form both these transform- ations belong to the same class. But no one except a person quite unaccustomed to view such changes will ascribe them to a vital power, although we admit they cor- respond remarkably to our common concep- tions of life; they are really chemical pro- cesses dependent upon the common chemical forces. Our notion of life involves something more than mere reproduction, namely, the idea of an active power exercised by virtue of a definite form, and production and gene- ration in a definite form. By chemical agency we can produce the constituents of muscular fibre, skin, and hair; but we can form by their means no organized tissue, no organic cell. The production of organs, the co-opera- tion of a system of organs, and their power not only to produce their component parts ; from the food presented to them, but to generate themselves in their original form and with all their properties, are characters belonging exclusively to organic life, and constitute a form of reproduction indepen- dent of chemical powers. The chemical forces are subject to the invisible cause by which this form is pro- duced. Of the existence of this cause itself we are made aware only by the phenomena which it produces. Its laws must be inves- tigated just as we investigate those of the other powers which affect motion and changes in matter. The chemical forces are subordinate to this cause of life, just as they are to elec- tricity, heat, mechanical motion, and fric- tion. By the influence of the latter forces, they suffer changes in their direction, an in- crease or diminution of their intensity, or a complete cessation or reversal of their action. Such an influence and no other is exer- t ised by the vital principle over the chemical forces; but in every case where combination or decomposition takes place, chemical affini- ty and cohesion are in action The vital principle is only known to us through the peculiar form cf its instruments, that is, through the organs in which it re- sides. Hence, whatever kind of energy a a substance may possess, if it is amorphous and destitute of organs from which the im- pulse, motion or change proceeds, it does not live. Its energy depend;? in this case on a chemical action. Light, heat, electricity, or other influences may increase, diminish, or arrest this action, but they are not its effi- cient cause. In the same way the vital principle go- verns the chemical powers in the living body. All those substances to which we apply the general name of food, and all the bodies formed from them in the organism, are che- mical compounds. The vital principle has, therefore, no other resistance to overcome, in order to convert these substances into component parts of the organism, than the chemical powers by which their constituents are held together. If the food possessed life, not merely the chemical forces, but this vitality, would offer resistance to the vital force of the organism it nourished. All substances adapted for assimilation are bodies of a very complex constitution; their atoms are highly complex, and are held together only by a weak chemical action. They are formed by the union of two or more simple compounds; and in proportion as the number of their atoms augments their disposition to enter into new combinations is diminished ; that is, they lose the power of acting chemically upon other bodies. Their complex nature, however, renders them more liable to be changed, by the agency of external causes, and thus to suffer decomposition. Any external agency, in many cases even mechanical friction, is sufficient to cause a disturbance in the equi- librium of the attraction of their constitu- ents ; they arrange themselves either into new, more simple, and permanent combina- tions, or if a foreign attraction exercise its influence upon it, they arrange themselves in accordance with that attraction. The special characters of food, that is, of substances fitted for assimilation, are absence of active chemical properties, and the capa- bility of yielding to transformations. The equilibrium in the chemical attrac- tions of the constituents of the food is dis- turbed by the vital principle, as we know it may be by many other causes. But the union of its elements, so as to produce new combinations and forms, indicates the pre- sence of a peculiar mode of attraction, and the existence of a power distinct from all other powers of nature, namely, the vital principle. All bodies of simple composition possess a greater or less disposition to form combi- nations. Thus oxalic acid is one of the simplest of the organic acids, while stearic acid is one of the most complex ; and the former is the strongest, the latter one of the POISONS, CONTAGIONS, MIASMS. 123 weakest, in respect to active chemical cha- racter. By virtue of this disposition, simple compounds produce changes in every body which offers no resistance to their action; they enter into combination and cause de- composition. The vital principle opposes to the con- tinual action of the atmosphere, moisture and temperature upon the organism, a re- sistance which is, in a certain degree, invin- cible. It is by the constant neutralization and renewal of these external influences that life and motion are maintained. The greatest wonder in the living organ- ism is the fact that an unfathomable wisdom has made the cause of a continual decom- position or destruction, namely, the support of the process of respiration, to be the means of renewing the organism, and of resisting all the other atmospheric influences, such as those of moisture and changes of tem- perature. When a chemical compound of simple constitution is introduced into the stomach, or any other part of the organism, it must exercise a chemical action upon all sub- stances with which it comes in contact ; for we know the peculiar character of such a body to be an aptitude and power to enter into combinations and effect decompositions. The chemical action of such a compound is of course opposed by the vital principle. The results produced depend upon the strength of their respective actions : either an equilibrium of both powers is attained, a change being effected without the destruc- tion of the vital principle, in which case a medicinal effect is occasioned ; or the acting body yields to the superior force of vitality, that is, it is digested; or lastly, the chemical action obtains the ascendency and acts as a poison. Every substance may be considered as nutriment) which loses its former properties when acted on by the vital principle, and does not exercise a chemical action upon the living organ. Another class of bodies change the direc- tion, the strength, and intensity of the re- sisting force, (the vital principle,) and thus exert a modifying influence upon the func- tions of its organs. They produce a dis- turbance in the system, either by their pre- sence, or by themselves undergoing a change ; these are medicaments. A third class of compounds are called poi sons, when they possess the property of uniting with organs or with their component parts, and when their power of effecting this is stronger than the resistance offered by the vital principle. ' The quantity of a substance and its con- dition must, obviously, completely change the mode of its chemical action. Increase of quantity is known to be equi- valent to superior affinity. Hence a medico- \ ment administered in excessive quantity may act as a poison, and a poison in small doses | as a medicament. Food will act as a poison, that is, it will produce disease, when it is able to exercise a chemical action by virtue of its quantity ; or, when either its condition or its presence retards, prevents, or arrests the motion ot any organ. A compound acts as a poison when all the parts of an organ with which it is brought into contact enter into chemical combination with it, while it may operate as a medicine, when it produces only a partial change. No other component part of the organism can be compared to the blood, in respect of the feeble resistance which it offers to exte- rior influences. The blood is not an organ which is formed, but an organ in the act of formation j indeed, it is the sum of all the organs which are being formed. The che- mical force and the vital principle hold each other in such perfect equilibrium, that every disturbance, however trifling, or from what- ever cause it may proceed, effects a change in the blood. This liquid possesses so little of permanence, that it cannot be removed from the body without immediately suffer- ing a change, and cannot come in contact with any organ in the body, without yielding to its attraction. The slightest action of a chemical agent upon the blood exercises an injurious^ influ- ence ; even the momentary contact with the air in the lungs, although effected through the medium of cells and membranes, alters the colour and other qualities of the blood. Every chemical action propagates itself through the mass of the blood ; for exam- ple, the active chemical condition of the constituents of a body undergoing decom- position, fermentation, putrefaction, or de- cay, disturbs the equilibrium between the chemical force and the vital principle in the circulating fluid. Numerous modifications in the composition and condition of the compounds produced from the elements of the blood, result from the conflict of the vital force with the chemical affinity, in their in - cessant endeavour to overcome one another. < All the characters of the phenomena of contagion tend to disprove the existence of life in contagious matters. They without doubt exercise an influence very similar to 1 some processes in the living organism; but \ the cause of this influence is chemical ac- tion, which is capable of being subdued by other chemical actions, by opposed agencies. Several of the poisons generated in the body by disease lose all their power when introduced into the stomach, but others are not thus destroyed. It is a fact very decisive of their chemical nature and mode of action, that those poi- sons which are neutral or alkaline, such as the poisonous matter of the contagious fever in cattle (typhus contagiosus rumintmtinmS) or that of the smail-pox, lose their whole power of contagion in the stomach; whilst that of sausages, which has an acid reac- tion, retains all its frightful properties under the same circumstances. . 124 AGRICULTURAL CHEMISTRY. In the former of these cases, the free acid present in the stomach destroys the action of the poison, the chemical properties of which are opposed to it ; whilst in the latter it strengthens, or at ail events does not offer any impediment to poisonous action. /Microscopical examination has detected peculiar bodies resembling the globules of the blood in malignant putrefying pus, in the matter of vaccine, &c. The presence of these bodies has given weight to the opinion, that contagion proceeds from the developement of a diseased organic life; and these formations have been regarded as the living seeds of disease. This view, which is not adapted to dis- cussion, has led those philosophers who are accustomed to search for explanations of phenomena in forms, to consider the yeast produced by the fermentation of beer as pos- sessed of life. They have imagined it to be composed of animals or plants, which nourish themselves from the sugar in which they are placed, and at the same time yield alcohol and carbonic acid as excrementitious matters.* It would perhaps appear wonderful if bodies, possessing a crystalline structure and geometrical figure, were formed during the processes of fermentation and putrefaction from the organic substances and tissues of organs. We know, on the contrary, that the complete dissolution into organic com- pounds is preceded by a series of trans- formations, in which the organic structures gradually resign their forms. Blood, in a state of decomposition, may appear to the eye unchanged ; and when we recognise the globules of blood in a liquid contagious matter, the utmost that we can thence infer is, that those globules have taken no part in the process of decomposi- tion. All the phosphate of lime may be removed from bones, leaving them trans- parent and flexible like leather, without the form of the bones being in the smallest de- gree lost Again, bones may be burned until they be quite white, and consist merely of a skeleton of phosphate of lime, but they will still possess their original form. In the same way processes of decomposition in the blood may affect individual constitu- ents only of that fluid, which will become destroyed and disappear, whilst its other parts will maintain the original form. Several kinds of contagion are propagated through the air: so that, according to the view already mentioned, we must ascribe life to a gas, that is, to an aeriform body. All the supposed proofs of the vitality of contagions are merely ideas and figurative representations, fitted to render the pheno- mena more easy of apprehension by our senses, without explaining them. These figurative expressions, with which we are so willingly and easily satisfied in all * Annalen der Pharmacie. Band xxix. S. 93 und 100. sciences, are the foes of all inquines into the mysteries of nature ; they are like the fata mwgana, which show us deceitful views of seas, fertile fields, and luscious fruits, but leave us languishing when we have- most need of what they promise. It is certain that the action of contagions is the result of a peculiar influence depend- ent on chemical forces, and in no way con- nected with the vital principle. This in- fluence is destroyed by chemical actions, and manifests itself wherever it is not sub- dued by some antagonist power. Its exist- ence is recognised in a connected series of changes and transformations, in which it causes all substances capable of undergoing similar changes to participate. An animal substance in the act of decom- position, or a substance generated from the component parts of a living body by disease, communicates its own condition to all parts of the system capable of entering into the same state, if no cause exist in these parts by which the change is counteracted or de- stroyed. Disease is excited by contagion. The transformations produced by the dis- ease assume a series of forms. In order to obtain a clear conception of these transformations, we may consider the changes which substances, more simply composed than the living body, suffer from the influence of similar causes. When pu- trefying blood or yeast in the act of trans- formation is placed in contact with a solu- tion of sugar, the elements of the latter substance are transposed, so as to form al- cohol and carbonic acid. A piece of the rennet- stomach of a calf in a state of decomposition occasions the elements of sugar to assume a different ar- rangement. The sugar is converted into lactic acid without the addition or loss of any element. (1 atom of sugar of grapes C12 H12 O12 yields two atoms of lactic acid=2 (C6 H6 O6.) When the juice of onions or of beet-root is made to ferment at high temperatures, lactic acid, mannite, and gum are formed. Thus, according to the different states of the transposition of the elements of the exciting body, the elements of the sugar arrange themselves in different manners, that is, dif- ferent products are formed. The immediate contact of the decompos- ing substance with the sugar is the cause by which its particles are made to assume new forms and natures. The removal of that substance occasions the cessation of the decomposition of the sugar, so that should its transformation be completed before the sugar, the latter can suffer no further change. In none of these processes of decomposi- tion is the exciting body reproduced; for the conditions necessary to its reproduction do not exist in the elements of the sugar. Just as yeast, putrefying flesh, and the stomach of a calf in a state of decomposi POISONS, CONTAGIONS, MIASMS. 125 tion, when introduced into solutions of sugar, effect the transformation of this sub- stance, without being themselves regene- rated; in the same manner, miasms and certain contagious matters produce diseases in the human organism, by communicating the state of decomposition of which they themselves are the subject, to certain parts of the organism, without themselves being reproduced in their peculiar form and na- ture during the progress of the decompo- sition. The disease in this case is not contagious. Now when yeast is introduced into a mixed liquid containing both sugar and glu- ten, such as wort, the act of decomposition of the sugar effects a change in the form and nature of the gluten, which is, in conse- quence, also subjected to transformation. As long as some of the fermenting sugar re- mains, gluten continues to be separated as yeast, and this new matter in its turn ex- cites fermentation in a fresh solution of sugar or wort. If the sugar, however, should be first decomposed, the gluten which remains in solution is not converted into yeast. We see, therefore, that the repro- duction of the exciting body here depends 1. Upon the presence of that substance from which it was originally formed j 2. Upon the presence of a compound which is capable of being decomposed by contact with the exciting body. If we express in the same terms the re- production of contagious matter in conta- gious diseases, since it is quite certain that they must have their origin in the blood, we must admit that the blood of a healthy indi- vidual contains substances, by the decompo- sition of which the exciting body or conta- gion can be produced. It must further be admitted, when contagion results, that the blood contains a second constituent capable of being decomposed by the exciting body. It is only in consequence of the conversion of the second constituent, that the original exciting bodv can be reproduced. A susceptibility of contagion indicates the presence of a certain quantity of this second body in the blood of a healthy individual. The susceptibility for the disease and its in- tensity must augment according to the quan- tity of that body present in the blood ; and in proportion to its diminution or disappear- ance, the course of the disease will change. When a quantity, however small, of con- tagious matter, that is of the exciting body, is introduced into the blood of a healthy in- dividual, it will be again generated in the blood, just as yeast is reproduced from wort. Its condition of transformation will be com- municated to a constituent of the blood ; and in consequence of the transformation suf- fered Hy this substance, a body identical with or similar to the exciting or contagious mat- ter will be produced from another consti- tuent substance of the blood. The quantity of the exciting body newly produced must constantly augment, if its lurther trans- formation or decomposition proceeds more slowly than that of the compound in the blood, the decomposition of which it effects. If the transformation of the yeast gene- rated in the fermentation of wort proceeded with the same rapidity as that of the parti- cles of the sugar contained in it, both would simultaneously disappear when the ferment- ation was completed. But yeast requires a much longer time for decomposition than sugar, so that after the latter has completely disappeared, there remains a much larger quantity of yeast than existed in the fluid at the commencement of the fermentation, yeast which is still in a state of incessant progressive transformation, and therefore possessed of its peculiar property. The state of change or decomposition which affects one particle of blood, is im- parted to a second, a third, and at last to all the particles of blood in the whole body. It is communicated in like manner to the blood of another individual, to that of a third person, and so on or in other words, the disease is excited in them also. It is quite certain that a number of pecu- liar substances exist in the blood of some men and animals, which are absent from the blood of others. The blood of the same individual contains, in childhood and youth, variable quantities of substances, which are absent from it in other stages of growth. The susceptibility of contagion by peculiar exciting bodies in childhood, indicates a propagation and re- generation of the exciting bodies, in con- sequence of the transformation of certain substances which are present in the blood, and in the absence of which no contagion could ensue. The form of a disease is termed benignant, when the tranformations are perfected on constituents of the body which are not essential to life, without the other parts taking a share in the decomposi- tion ; it is termed malignant when they affect essential organs. It cannot be supposed that the different changes in the blood, by which its constitu- ents are converted into fat, muscular fibre, substance of the brain and nerves, bones, hair, &c., and the transformation of food into blood, can take place without the simulta- neous formation of new compounds which require to be removed from the body by the organs of excretion. In an adult these excretions do not vary much either in their nature or quantity. The food taken is not employed in increasing the size of the body, but merely for the pur- pose of replacing any substances which may be consumed by the various actions in the organism ; every motion, every manifesta- tion of organic properties, and every organic action being attended by a change in the material of the body, and by the assumption of a new form by its constituents.* * The experiments of Barruel upon the dif- ferent odours emitted from blood on the addition L2 126 AGRICULTURAL CHEMISTRY. But in a child this normal condition of sustenance is accompanied by an abnormal condition of growth and increase in the size of the body, and of each individual part of it. Hence there must be a much larger quantity of foreign substances, not belong- ing to the organism, diffused through every part of the blood in the body of a young individual. When the organs of secretion are in pro- per action, these substances will be re- moved from the system ; but when the func- tions of those organs are impeded, they will remain in the blood or become accumulated in particular parts of the body. The skin, lungs, and other organs, assume the func- tions of the diseased secreting organs, and the accumulated substances are eliminated by them. If, when thus exhaled, these sub- stances happen to be in the state of progres- sive transformation, they are contagious; that is, they are able to produce the same state of disease in another healthy organism, provided the latter organism is susceptible of their action or in other words, contains a matter capable of suffering the same pro- cess of decomposition. The production of matters of this kind, which render the body susceptible of conta- gion, may be occasioned by the manner of living, or by the nutriment taken by an in- dividual. A superabundance of strong and otherwise wholesome food may produce them, as well as a deficiency of nutriment, uncleanliness, or even the use of decayed substances as food. All these conditions for contagion must be considered as accidental. Their formation and accumulation in the body may be pre- vented, and they may even be removed from it without disturbing its most important functions of health. Their presence is not necessary to life. The action, as well as the generation of the matter of contagion is, according to this view, a chemical process participated in by all substances in the living body, and by all the constituents of those organs in which the vital principle does not overcome the chemical action. The contagion, accord- ingly, either spreads itself over every part of the body, or is confined particularly to certain organs, that is, the disease attacks all the organs or only a few of them, ac- cording to the feebleness or intensity of their resistance. In the abstract chemical sense, reproduc- tion of a contagion depends upon the pre- sence of two substances, one of which be- comes completely decomposed, but commu- nicates its own state of transformation to the second. The second substance thus of sulphuric acid, prove that peculiar substances are contained in the blood of different individuals ; the blood of a man of a fair complexion and that of a man of dark complexion were found to yield different odours ; the blood of animals also dif- fered in this respect very perceptibly from that of thrown into a state of decomposition is the 1 newly-formed contagion. The second substance must have been originally a constituent of the blood : the first may be a body accidentally present; but it may also be a matter necessary to life. If both be constituents indispensable for the support of the vital functions of certain principal organs, death is the consequence of their transformation. But if the abronce of the one substance which was a constitu- ent of the blood do not cause an immediate cessation of the functions of the most im- portant organs, if they continue in their action, although in an abnormal condition, convalescence ensues. In this case the pro- ducts of the transformations still existing in the blood are used for assimilation, and at this period secretions of a peculiar nature are produced. When the constituent removed from the blood is a product of an unnatural manner of living, or when its formation takes place only at a certain age, the susceptibility of contagion ceases upon its disappearance. The effects of vaccine matter indicate that an accidental constitution, of the blood is destroyed by a peculiar process of decom- position, which does not affect the other constituents of the circulating fluid. If the manner in which the precipitated yeast of Bavarian beer acts (page 107) be called to mind, the modus operandi of vac- cine lymph can scarcely be matter of doubt. Both the kind of yeast here referred to and the ordinary ferment are formed from gluten, just as the vaccine virus and the matter of small pox are produced from the blood. Ordinary yeast and the virus of human small-pox, however, effect a violent tumultuous transformation, the former in vegetable juices, the latter in blood, in both, of which fluids respectively their constitu- ents are contained, and they are reproduced from these fluids with all their charac- teristic properties. The precipitated yeast of Bavarian beer on the other hand acts en- tirely upon the sugar of the fermenting liquid and occasions a very protracted de- composition of it, in which the gluten which, is also present takes no part. But the air exercises an influence upon the latter sub- stance, and causes it to assume a new form and nature, in consequence of which this kind of yeast also is reproduced. The action of the virus of cow-pox is analogous to that of the low yeast ; it com- municates its own state of decomposition to a matter in the blood, and from a second matter is itself regenerated, but by a totally different mode of decomposition; the pro- duct possesses the mild form, and all the properties of the lymph of cow-pox. The susceptibility of infection by the virus of human small-pox must cease after vacci- nation, for the substance to the presence of which this susceptibility is owing has been removed from the body by a peculiar pro- cess of decomposition artificially excited. POISONS, CONTAGIONS, MIASMS. 127 But this substance may be again generated in the same individual, so that he may again become liable to contagion, and a second or a third vaccination will again remove the peculiar substance from the system. Chemical actions are propagated in no organs so easily as in the lungs, and it is well known that diseases of the lungs are above all others frequent and dangerous. If it is assumed that chemical action and the vital principle mutually balance each other in the blood, it must farther be sup- posed that the chemical powers will have a certain degree of preponderance in the lungs, where the air and blood are in imme- diate contact ; for these organs are fitted by nature to favour chemical action; they offer no resistance to the changes experienced by the venous blood. The contact of air with venous blood is limited to a very short period of time by the motion of the heart, and any change be- yond a determinate point is, in a certain degree, prevented by the rapid removal of the blood which has become a-rterialised. Any disturbance in the functions of the heart, and any chemical action from with- out, even though weak, occasions a change in the process of respiration. Solid sub- stances also, such as dust from vegetable, animal, or inorganic bodies, act in the same way as they do in a saturated solution of a salt in the act of crystallization, that is, they occasion a deposition of solid matters from the blood, by which the action of the air upon the latter is altered or prevented. When gaseous and decomposing sub- stances, or those which exercise a chemical action, such as sulphuretted hydrogen and carbonic acid, obtain access to the lungs, they meet with less resistance in this organ than in any other. The chemical process of slow combustion in the lungs is accele- rated by all substances in a state of decay or putrefaction, by ammonia and alkalies ; but it is retarded by empyreumatic sub- stances, volatile oils, and acids. Sulphu- retted hydrogen produces immediate decom- position of the blood, and sulphurous acid combines with the substance of the tissues, the cells, and membranes. When the process of respiration is modi- fied by contact with a matter in the pro- gress of decay, when this matter commu- nicates the state of decomposition, of Avhich it is the subject, to the blood, disease is pro- duced. If the matter undergoing decomposition is the product of a disease, it is called con- I tagion; but if it is a product of the decay ! or putrefaction of animal and vegetable I substances, or if it acts by its chemical pro- perties, (not by the state in which it is,) and therefore enters into combination with parts of the body, or causes their decomposition, it is termed miasni. Gaseous contagious matter is a miasm emitted from blood, and capable of gene- rating itself again in blood But miasm, properly so called, causes disease without being itself reproduced. All the observations hitherto made upon gaseous contagious matters prove, that they also are substances in a state of decompo- sition. When vessels filled with ice are placed in air impregnated with gaseous con- tagious matter, their outer surfaces become covered with water containing a certain quantity of this matter in solution. This water soon becomes turbid, and in common language putrefies, or, to describe the change more correctly, the state of decomposition of the dissolved contagious matter is com- pleted in the water. All gases emitted from putrefying animal and vegetable substances in processes of disease, generally possess a peculiar nau- seous offensive smell, a circumstance which, in most cases, proves the presence of a body in a state of decomposition. Smell itself may in many cases be considered as a re- action of the nerves of smell, or as a resist- ance offered by the vital powers to chemical action. Many metals emit a peculiar odour when rubbed, but this is the case with none of the precious metals, those which suffer no change when exposed to air and moisture- Arsenic, phosphorus, musk, the oils of lin- seed, lemons, turpentine, rue, and pepper- mint, possess an odour only when they are in the act of eremacausis (oxidation at com- mon temperatures.) The odour of gaseous contagious matters is owing to the same cause ; but it is also generally accompanied by ammonia, which may be considered in many cases as the means through which the contagious matter receives a gaseous form, just as it is the means of causing the smell of innumerable substances of little volatility, and of many which have no odour. (Robiquet.)* Ammonia is very generally produced in cases of disease ; it is always emitted in those in which contagion is generated, and is an invariable pioductof the decomposition of animal matter. The presence of ammo- nia in the air of chambers in which diseased patients lie, particularly of those afflicted with a contagious disease, may be readily detected; for the moisture condensed by ice in the manner just described, produces a white precipitate in a solution of corrosive sublimate, just as a solution of ammonia does. The ammoniacal salts also, which are obtained by the evaporation of rain- water after an acid has been added, when treated with lime so as to set free their am- monia, emit an odour most closely resem- bling that of corpses, or the peculiar smell of dunghills. By evaporating acids in air containing gaseous contagions, the ammonia is neu- tralised, and we thus prevent further de- composition, and destroy the power of the contagion, that is, its state of chemical * Ann. de Chim. et de Phya. XV. 27. 128 AGRICULTURAL CHEMISTRY. change. Muriatic and acetic acids., and in several cases nitric acid, are to be preferred for this purpose before all others. Chlorine also is a substance which destroys ammonia and organic bodies with much facility ; but it exerts such an injurious and prejudicial influence upon the lungs, that it may be classed amongst the most poisonous bodies known, and should never be employed in places in which men breathe. Carbonic acid and sulphuretted hydrogen, which are frequently evolved from the earth in cellars, mines, wells, sewers, and other places, are amongst the most pernicious mi- asms. The former may be removed from the air by alkalies, the latter, by burnin^ sulphur, (sulphurous acid,) or by the evapo- ration of nitric acid. The characters of many organic com- pounds are well worthy of the attention and study both of physiologists and pathologists, more especially in relation to the mode of action of medicines and poisons. Several of such compounds are known, which to all appearance are quite indifferent substances, and yet cannot be brought into contact with one another in water without suffering a complete transformation. All substances which thus suffer a mutual de- composition, possess complex atoms ; they belong to the highest order of chemical com- pounds. For example, amygdalin, a con- stituent of bitter almonds, is a perfectly neu- tral body, of a slightly bitter taste, and very easily soluble in water. But when it is in- troduced into a watery solution of synaptas, (a constituent of sweet almonds,) it disap- pears completely without the disengagement of any gas, and the water is found to con- tain free hydrocyanic acid, hydruret of ben- zule (oil of bitter almonds,) a peculiar acid and sugar, all substances of which merely the elements existed in the amygdalin. The same decomposition is effected when bitter almonds, which contain the same white matter as the sweet, are rubbed into a pow- der and moistened with water. Hence it happens that bitter almonds pounded and digested in alcohol, yield no oil of bitter al- monds containing hydrocyanic acid, by dis- tillation with water ; for the substance which occasions the formation of those volatile sub- stances, is dissolved by alcohol without change, and is therefore extracted from the pounded almonds. Pounded bitter almonds contain no amygdalin, also, after having been moistened with water, for that sub- stance is completely decomposed when they are thus treated. No volatile compounds can be detected by their smell in the seeds of the Sinapis alba and S. nigra. A fixed oil of a mild taste is obtained from them by pressure, but no trace of a volatile substance. If, however, the seeds are rubbed to a fine powder, and sub- jected to distillation with water, a volatile oil of a very pungent taste and smell passes over along with the steam. But if, on the contrary, the seeds are treated with alcohol previously to their distillation w/th water, tne residue does not yield a volatile oil. The alcohol contains a crystalline body called sinapin, and several other bodies. Those do not possess the characteristic pungency of the oil, but it is by the contact of them with water, and with the albuminous constituents of the seeds, that the volatile oil is formed. Thus bodies regarded as absolutely indif- ferent in inorganic chemistry, on account of their possessing no prominent chemical characters, when placed in contact with one another, mutually decompose each other. Their constituents arrange themselves in a peculiar manner, so as to form new com- binations \ a complex atom dividing into two or more atoms of less complex constitution, in consequence of a mere disturbance in the attraction of their elements. The white constituents of the almonds and mustard, which resemble coagulated al- bumen, must be in a peculiar state in order to exert their action upon amygdalin, and upon those constituents of mustard from which the volatile pungent oil is produced. If almonds, after being blanched and pounded, are thrown into boiling water, or treated with hot alcohol, with mineral acids, or with salts of mercury, their power to effect a decomposition in amygdalin is com- pletely destroyed. Synaptas is an azotised body which cannot be preserved when dis- solved in water. Its solution becomes rapidly turbid, deposits a white precipitate, and acquires the offensive smell of putrefy- ing bodies. It is exceedingly probable that the pecu- liar state of transposition into which the ele- ments of synaptas are thrown when dis- solved in water, may be the cause of the decomposition of amygdalin, and formation of the new products arising from it. The action of synaptas in this respect is very similar to that of rennet upon sugar. Malt, and the germinating seeds of corn in general, contain a substance called dias- tase, which is formed from the gluten con- tained in them, and cannot be brought in contact with starch and water, without effect- ing a change in the starch. When bruised malt is strewed upon warm starch made into a paste with water, the paste after a few minutes becomes quite liquid, and the water is found to contain, in place of starch, a substance in many respects similar to gum. But when more malt is added and the heat longer continued, the liquid acquires a sweet taste, and all the starch is found to be converted into sugar of rapes. The elements of diastase have at the same time arranged themselves into new combina- tions. The conversion of the starch contained in 7 ood into sugar of grapes in diabetes indi- cates that amongst the constituents of some one organ of the body a substance or sub- stances exist in a state of chemical action, to which the vital principle of the diseased POISONS, CONTAGIONS, MIASMS. 129 organ opposes no resistance. The compo- nent parts of the organ must suffer changes simultaneously with the starch, so that the more starch is furnished to it, the more ener- getic and intense the disease must become ; while if only food which is incapable of suffering such transformations from the same cause is supplied, and the vital energy is strengthened by stimulant remedies and nourishment, the chemical action may finally be subdued, or, in other words, the disease cured. The conversion of starch into sugar may also be effected by pure gluten, and by dilute mineral acids. From all the preceding facts, we see that very various transpositions, and changes of composition and properties, may be pro- duced in complex organic molecules, by every cause which occasions a disturbance in the attraction of their elements. When moist copper is exposed to air con- taining carbonic acid, the contact of this acid increases the affinity of the metal for* the oxygen of the air in so great a degree that they combine, and the surface of the copper becomes covered with green carbo- nate of copper. Two bodies, which pos- sess the power of combining together, as- sume, however, opposite electric conditions at the moment at which they come in contact. When copper is placed in contact with iron, a peculiar electric condition is excited, in consequence of which the property of the copper to unite with oxygen is destroyed, and the metal remains quite bright. When formate of ammonia is exposed to a temperature of 388 F. (180 C.) the in- tensity and direction of the chemical force undergo a change, and the conditions under which the elements of this compound are enabled to remain in the same form ceases to be present. The elements, therefore, ar- range themselves in a new form; hydro- cyanic acid and water being the result of the change. Mechanical motion, friction, or agitation, is sufficient to cause a new disposition of the constituents of fulminating silver and mercury, that is, to effect another arrange- ment of their elements, in consequence of which, new compounds are formed. We know that electricity and heat possess a decided influence upon the exercise of chemical affinity; and that the attractions of substances for one another are subordi- nate to numerous causes which change the condition of these substances, by altering the direction of their attractions. In the same manner, therefore, the exercise of chemical powers in the living organism is dependent upon the vital principle. The power of elements to unite together, and to form peculiar compounds, which are generated in animals and vegetables, is chemical affinity ; but the cause by which they are prevented from arranging them- selves according to the degrees of their natu- ral attractions the cause, therefore, by which they are made to assume their pecu- liar order and form in the body is the vital principle. After the removal of the cause which forced their union that is, after the extinc- tion of life most organic atoms retain their condition, form, and nature, only by avisin- erticB ; for a great law of nature proves that matter does not possess the power of spon- taneous action. A body in motion loses its motion only when a resistance is opposed to it ; and a body at rest cannot be put in mo- tion, or into any action whatever, without the operation of some exterior cause. The same numerous causes which are opposed to the formation of complex organic molecules, under ordinary circumstances, occasion their decomposition and transform- ations when the only antagonist power, the vital principle, no longer counteracts the 10- fluence of those causes. Contact with air and the most feeble chemical action now effect changes in the complex molecules; even the presence of any body the particles of which are undergoing motion or transpo- sition, is often sufficient to destroy their state of rest, and to disturb the statical equilibrium in the attractions of their constituent ele- ments. An immediate consequence of this is that they arrange themselves according to the different degrees of their mutual attrac- tions, and that new compounds are formed in which chemical affinity has the ascend- ency, and opposes any further change, while the conditions under which these compounds were formed remained unaltered. TABLES: SHOWING THE PROPORTION BETWEEN THE HESSIAN AND ENGLISH STANDARD OP WEIGHTS AND MEASURES. IN general all the weights and measures employed in this edition are those of the English standard. In a few cases only, the Hessian weights and measures have been retained. In these the numbers do not re- present absolute quantities, but are merely intended to denote a proportion to other numbers. This has been done to avoid any unnecessary intricacy in the calculations, and to present whole numbers to the reader, without distracting his attention by decimal parts. For those, however, who wish to be acquainted with the exact English quanti- ties, a table is given below. 1 Ib. English is equal to 0-90719 Ibs. Hes- sian; hence, about one-tenth less than the latter. 1 Ib. Hessian is equal to M02 Ibs. English. 2 Ibs. Hessian are equal to 2'204 ' 3-306 4-409 5-511 6-612 7-716 8-318 130 AGRICULTURAL CHEMISTRY. 9 IDS 10 20 - 30 40 - 50 fin . Hessian are equal to 9*92 Ibs. Eng - - . - 11-02 - 22-04 . 33-06 - 44-09 55-11 . fifi-19 lish. i c < i t figures, the whole series given in the case of the pounds will also be obtained. 1 Sq. foot Hessian is equal to 0'612 Sq. foot Eng. 2 feet .... 1-345 70 on - 77-16 OQ.1 90 100 - 200 300 - 400 500 - 600 700 - 800 900 - 1000 99-29 110-2 - 220-4 330-6 . 440-9 551-1 - 661-2 771-6 - 881-8 992-0 . 1102-0 CUBIC FEET. One English cubic foot contains 1-81218 of a Hessian cubic foot; the Hessian and English cubic inch may be considered as equal, one English cubic inch containing 1-048715 Hessian cubic inch. SQUARE FEET. The Hessian acre is equal to 40,000 Hes- sian square feet, or 26,911 English square feet; 1 English square foot being equal to 1-4864 Hessian. The following is a table to save the trouble of calculation. The table is only stated to the figure 10, but by removing the decimal point one or two 1 cub. foot Hessian is eq. to 0-551 cub. foot Eng. 2 feet - - . . 1-103 " 3 .... 1-665 feet 4 2-207 5 .... 2-759 " 6 3-311 " 7 .... 3-863 " 8 4-415 " 9 .... 4-966 " 10 5-518 " THE END. INDEX. A. Absorption, by roots, 37 Of salts, 39. Acid, acetic, emitted by plants, 51 transforma- tion of, 92 formation of, 100, 102 Boracic, 42 Carbonic, 10 contained in the atmo- sphere, 11 decomposed by plants, 16 from respiration, 16 why necessary to plants, 36 Cyanic, transformation of, 94 Formic, 25, 26, *88 Hippuric, 33 Humic, 12 proper- ties of, 13 Hydrocyanic, 25, 88 Kinic, 39 Lactic, 64 production of, 98 Meconic, 39 Melanic, 99 Nitric, source of, 32 Phosphoric, in ashes of plants, 53 Rocellic, in plants, 37 Succinic, 112 Sulphuric, action of, on soils, 70, 84 Tartaric, in grapes, 37. Acids, action of upon sugar, 92 Arrest decay, 111 Capacity for saturation, 36 Organic, in plants, 11, 36 when formed, 18. Affinity, action of, 25 Chemical, examples of, 88 Weak, example of, 88. Agave Americana, absorbs oxygen, 18. Agriculture, in China, 65 Object of, 34, 49, 57 how attained, 49 Its importance, 49 A principle in, 63. Air, access of, favoured, 27 Ammonia in, 11, 32 Carbonic acid in, 15 Effect of upon juices, 100 on soils, 56 Improved by plants, 17 Necessary to plants, 44. Albumen, 33. Alcohol, effect of heat on, 93 Exhaled, 25 Products of its oxidation, 99 From sugar, 95. Aldehyde, 99. Alkalies, from granitic soils, 40 Presence of, in- dicated, 72 Promote decay in wood, 111 Quantity in aluminous minerals, 50. Alkaline bases, in plants, on what their existence depends, 38 Salts in plants, sources of, 51 contained in fertile soils, 52. Alloxan, 108. Alloxantin, 108. Alumina, in fertile soils, 49 Its influence on vegetation, 49. Amber, origin of, 112. Ammonia, carbonate of, from wine, 64 how fixed, 64 Cause of nitrification, 103 Changes co- lours, 30 Condensed by charcoal, 35 Con- version of, into nitric acid, 103 Early exist- ence of, 42 Fixed by gypsum, 64 From animals, 58 Contained in beet-root, &c., 32 maple juice, 33 stables, &c., 64 Fur- nishes nitrogen, 36 Loss from evaporation, 34 Produced by animal organism, 42 Pro- duct of decay, 30 disease, 129 Properties of, 31 Quantity absorbed by charcoal, 35 by decayed wood, 35 In rain-water, 31 Se- parated from soils by rain, 35 In snow water, 32 Solubility of, 91 Transformation of, 30. Amylin, its effect, 26. Analysis of decayed wood, 111 Of fire-damp, 115 Of guano, 67 Of lentils, 54 Of oak- wood, 110 Of night-soil, 60 Of salt water; 43 Of soils, 73 113 Of wood coal, 113 Animal food, preservation of, 101 Life, con- nexion of, with plants, 9 Bodies, products of decay, 30. Animals, excrements of, 18, 63. Annual plants, how nourished, 46. Anthoxanthum odoratum, acid in, 33. Anthracite, 115. Antidotes to poisons, 118. Apatite, 53. Arable land, 50. Aromatics, their influence on fermentation, 105. Argillaceous earth, its origin, 50. Arragonite, transformation of, 90. Arsenious acid, action of, 118. Ashes, as manure, 67 Comparative value of, 61 Of fire-wood, 38 Of pine trees, 37 Of plants, origin of salt in, 43 Importance of ex- amination of, 38 Of wheat, 53 used as a manure, 72 Of bones, 62 Of peat, 62 Of coals, 67. Assimilation, of carbon, 12, 23 Of carbonic acid, and ammonia, 45 Of hydrogen, 28, 29 Of nitrogen, 30, 36 Its power, 48. Atmosphere, ammonia in, 11, 32 Composition of, 11 How maintained, 16 Composition is invariable, 15 Carbonic acid in the, 11 Mo- tion of, 17. Atoms, motions of 89 Permanence in position of, 89. Attraction, powerful, overcome, 94. Azotised matter in juices of plants, 47 Sub- stances, combustion of, 102. B. Bamboo, silica in, 58. Bark of trees, products in, 18. Barley, analysis of, 53. Barruel, his experiments on the blood, 125. Base, what, 36. Bases, alkaline, in plants, on what their existence depends, 38 Organic, 11 Oxygen contained in, 36 In plants, 37 Substitution of, 37. Beans, alkalies in, 54 Nutritive power of, 54 Becquerrel, experiments of, 51. Beech, ashes of, 30. Beer, 107 109 Bavarian, 107 Varieties of 106. Beet-root sugar, 14 Ammonia from, 32 From sandy soils, 47. Benignant disease, 126. Benzoic acid, formed, 33. Birch tree, ammonia from, 33. Blood, its office, 46 Action of chemical agents upon, 123 Its feeble resistance to exterior in- fluences, 123 Organic salts in, 116 Its cha- racter, 120. Blossoms, when produced, 24 Increased, 45 Removal of, from potatoes, 46. 132 INDEX. Bones, dust of, 62 Durability of, 68 Gelatine in, 68 Use in compost, 72. Bouquet of wines, 105. Boracic acid, 41. Botanists, neglect of chemistry by, 20. Brandy, from corn, 105 Oil of, 105. Brazil, wheat in, 52. Brown coal, 113. Buckwheat, ashes of, 54. Bulbs, how nourished, 27. C. Calcareous spar, 90. Calcium, fluoride of, 53 Chloride of, 64. Calculous disorders, 26. Calico printing, use of cow-dung in, 63. Caoutchouc, in plants, 27. Carbon, 10 Afforded to the soil by plants, 27 Assimilation of, 12-23 Combination of, with Oxygen, 10 Of decaying substances seldom affected by oxygen, 111 Derived from air, 16 In decaying wood, 111 In decaying woody fibre, 111 In sea-water, 16 Oxide of, formed, 92 Quantity in grain, 14 in land, 14 in straw, 14 Restored to the soil, 27 Received by leaves, 16 Its affinity for oxygen, 100. Carbonate of ammonia decomposed by gypsum, 34 Of lime in caverns and vaults, 43. Carbonic acid in the atmosphere, 11 Changes in the leaves, 48 Decomposed by plants, 16 Emission of, at night, 18 Evaporation of, 20 Evolution from decaying bodies, 100 From decaying plants, 29 excrements, 34 humus, 23 respiration, 25 springs, 29 woody fibre, 23 Increase of, prevented, 16 Influence of light on its decomposition, 19. Carburetted hydrogen with coal, 115. Caverns, stalactites in, 43. Charcoal condenses ammonia, 35 Experiments of Lukas on, 84 May replace humus, 27 Theory of its action, 27 Promotes growth of plants, 84. Chemical effects of light, 48 Forces can replace the vital principle, 26 Processes in nutrition of vegetables, 9 Transformations, 25, 87. Chemistry, definition of, 9 Organic, what is, 9 Neglected by botanists, 20; and physiolo- gists, 20. China, its agriculture, 65 Collection and use of manure in, 65. Chloride of calcium, 64 Of nitrogen, 88 Of potassium, its effect, 39 Of sodium, its vola- tility, 42. Clay, burned, advantages of, as a manure, 35. Clays, potash in, 50. Clay slate, 53. Coal, formation of, 113 Inflammable gases from 115 Origin of substances in, 112 Of humus 12, 44 Wood or brown, 113. Colours of flowers, 33. Combustion at low temperatures, 100 Of de cayed wood, 112 Induction of, 102 Re moves oxygen, 16 Spontaneous, 94. Compost manure. 72. Concretions from horses, 53. Constituents of plants, 10. Contagions, reproduction of, on what dependent 121 Susceptibility to, how occasioned, 125. Contagions, how produced, 121 Propagation of 124. Contagious matters, action of, 124, 122, 129 Their effects explained, 121 Life in, disproved 121 Reproduction of, 121. Copper alloy, its action, on sulphuric acid, 88. )orn, how cultivated in Italy, 52 Phosphate of magnesia in, 53. }orn brandy, 105. Corrosive sublimate, action of, 118. 2ow, excrements of the, 41, 59, 60 Variable in value, 60 Urine of the, 60 ; rich in potash, 41. }ow-pox, action of virus of, 127. >ops, rotation of, 54 Favorable effects of, 55 Principles regulating, 59. Cultivation, its benefits, 17 Different methods of, 49 Object of, 49. Culture, art of, 43 Of plants, principles of the, 49. Cyanic acid, transformation of, 94. Cyanogen, combustion of, 102 Transformation of, 94. D. Davis, his account of Chinese manure, 65.. Death from nutritious substances, 21 The source of life, 36. Decandolle, his theory of excretion, 55 Dif- ference of his views and those of Macaire- Princep, 56. Decay, 98 A source of ammonia, 30 Of wood, 109 Of plants restores oxygen, 29 and pu- trefaction, 88. Decomposition, 24, 87 Organic, chemical, 83. Dextrine, 21. Diamond, its origin, 112. Diastase, 46 Contains nitrogen, 46. Disease, how excited, 120. Dog, excrement of the, 59. Dunghills, liquid from, 64 Reservoirs, 64. E. Ebony wood, oxygen and hydrogen in, 19. Effete matters separated, 24. Elements of plants, 10 Not generated by or- gans, 21. Elphinstone, Sir Howard, on soda-ash, as a ma- nure, 69. Equilibrium of attractions disturbed, 92. Equisetacse contain silica, 58. Eremacausis, 98 Analogous to putrefaction, 130 Arrested, 98 Definition of, 98 Necessary to nitrification, 102 Of bodies containing ni- trogen, 102 Of bodies destitute of nitrogen, 100. Ether, oenanthic, 105. Excrementitious matter, production of, illus- trated, 25. Excrement, animal, its chemical nature, 59 Of the dog, cow, &c., 59 Influence of, as ma- nure, 61. Excrements of plants, 55 Conversion of, into humus, 13 Of man, amount of, 65 Value of, 63 Propagation of, 65. Excretion, organs of, 25 Of plants, theory of, 55. Experiments in physiology, object of, 20 Of physiologists not satisfactory, 22. F. Fallow, changes from, 52 Crops, 54 Time, 54, Fattening of animals, 49. Faeces, analysis of, 60. Ferment, 95, 103. INDEX. 133 Fermentation, 103 Of Bavarian beer, 107 Of beor, 107 Gay-Lussac's experiments in, 101 Of sugar, 95 Of vegetable juices, 95 Vinous, 103 Of wort, 104. Fertility of fields, how preserved, 61. Fires, plants on localities of, 52. Fir-wood, analysis of its ashes, 38. Fishes in salt-pans, 41. Flanders, manure in, 65. Fleabane, 54. Flesh, effect of salt on, 116. Flour, bran of, 62. Flowers, colours due to ammonia, 33. Fluorine in ancient bones, 53. Food, effects on products of plants, 47 Of young plants, 45 Transformation and assimilation of, 25. Formation of wood, 47. Formic acid, theory of its formation, 25 From hydrocyanic acid, 25. Fossil resin, origin of, 112. Franconia, caverns in, 43. Fruit, increased, 45 Ripening of, 29 Changes attending, 45. Fulminating silver, 88. G. Gaseous substances in the lungs, effect of, 126. Gasterosteus aculeatus, in salt-pans, 41. Gay-Lussac, his experiments, 101. Germany, cultivation in, 61. Germination of potatoes, 45 Of grain, 46. Glass as a manure, 63. Glue, manure from, 62. Gluten, conversion of, into yeast, 106-110 Decomposition of, 98 Gas from, 103. Grain, germination of, 46 Manure for, 40 Rust in, 75. Granitic soil affords alkalies, 40. Grapes, fermentation of, 103 Juice of, differences in, 106 Potash in, 38. Grasses, seeds of, follow man, 41 Silica in, 58 Valued in Germany, 57. Grauwacke, soil from, 50. Growth of plants, conditions for the, 48. Guano, 67. Gypsum, decomposition of, 34 84 Its influence, 34 Use of, 64. H. Hay, carbon in, 14 Contains nitrogen, 59 Silica, 53. Haystack, effect of lightning upon a, 53. Hessian and English Aveights and measures, 130. Hibernating animals, 45. Horse, urine of the, 35 Concretions in the, 53. Horse dung, action of water upon, 60 Analysis of, 60. Human ffeces, analysis of, 60. Humate of lime, quantity received by plants, 13. Humic acid, 12, 31 Action of, 44 Properties of, 13 Is not contained in soils, 31 Quantity received by plants, 14 Insolubility of, 44. Humus, 11 Action of, 23 Analysis of, 12 Erroneous opinions concerning, 17 Action upon oxygen, 43 Coal of, 44 Conversion of woody fibre into, 110 How produced, 110 Its insolubility, 43 Properties of, 13 Re- placed by charcoal, 27 Source of carbonic acid, 23 Theory of its action, 23 Unneces- sary for plants, 27. Hydrocyanic acid, 23, 88. Hydrogen, assimilation of, 28, 29 Properties of, 10 Excess of, in wood accounted for, 28 Of decayed wood, 111 Of plants, source of, 28 Peroxide of, 89. Hyett, Mr., on nitrate of soda, 69, I. Ice, bubbles of gas in, 20. Indifferent substances, 1 1. Ingenhouss, his experiments, 18. Inorganic compounds, 91 Action of, 115 In what they differ from organic, 91. Inorganic constituents of plants, 3643. Iron, oxide of, attracts ammonia, 35. Irrigation of meadows, effect of, 43-57. L. Lactic acid, production of, 98. Lava, soil from, 51. Lead, salts of, compounds with organic matter, 118. Leaves, absorb carbonic acid, 16 Ashes of, con- tain alkalies, 52 Cessation of their functions, 24 Change colour from absorption of oxygen, 24 Consequence of the production of their green principle, 58 Decompose carbonic acid, 48 Their office, 46 Power of absorbing nu- triment, how increased. 24 Quantity of car- bon received by, 16 Contain a^otised matter, 63. Lentils, analysis of, 54. Life, notion of, 121. Light, absence of, its effect, 18 Chemical effects of, 48 Influences decomposition of carbonic acid, 19. Lime, phosphate of, 62, 71. Lucerne, phosphate of lime in, 54 Benefits at- tending its culture, 58. . M. Macaire-Princep, his experiments, 55. Magnesia, phosphate of, in seeds, 22. Manure, 59-70 Animal, yields ammonia, 33 Artificial, 69-71 Carbonic acid from, 34 Components of, should be known, 49 Of the Chinese, 65 Effect of, 59 Bone, 62. Maple juice, ammonia from, 33 Trees, sugar of, 33. Meadows, irrigation of, 43 57. Medicine, action of, remedies in, 62. Mellitic acid, 112. Metallic compounds required by plants, 21. Metamorphosis, 88. Miasm, defined, 127. Minerals attract ammonia, 35. Morbid poisons, 121. Motion, its influence on chemical forces, 89. Mould, vegetable, 112 Conversion of woody fibre into, 112. Mouldering of bodies, 113. Must, fermentation of, 104. N. Naples, soils of, 52. Night-soil, 65. Nile, soil of its vicinity, 57. Nitrate of soda, as a manure, 69. Nitric acid from ammonia, 103 animals, 30-* How formed, 102. Nitrification, 102 Condition for, 103. Nitrogen from animals, 30 Account of, 10- M 134 INDEX. Application of substances containing it, 34 Assimilation of, 30-36 Chloride of, 88 Compounds of, 11 peculiarity in 97 In ex- crements, 63 From the atmosphere, 30 In plants, 11 Production of, the object of agri- culture, 34 Transformation of bodies con- taining, 93 In rice, 33 In solid excrements, 63 In urine, 64. Nutrition, conditions essential to, 21 Inorganic substances required in, 21 Superfluous, how employed, 24 Of young plants, 58. O. Oaks, ashes of, 52 Excretions of, 18 Dwarf, 23. Oak-wood affords humic acid, 13 Composition of, 110 Mouldered, analysis of, 111. Odour of substances, 106 Of gaseous contagious matter, 127. CEnanthic ether, 105. Organs of excretion, 25. . Organic acids, 11 Decomposition of, 49 Che- mistry, 9 Compounds, 29 Compared with inorganic salts in plants, 91. Organised bodies do not generate substances, 24. Oxamide, decomposition of, 121. Oxides, metallic, in fir-wood, 38. Oxygen, action on alcohol, 99 Absorption of, at night, 18 by leaves, 18 respiration, 25 plants, 18 wood, 110 Action upon woody fibre, 111 Its action in decomposition, 101 Emitted by leaves, 15 Given to air by land, 28 Extracted from air by mould, 112 In air, 11 Consumption of, 15 In water, 28 Pro- motes decay, 44 Separated during the forma- tion of acids, 29 Is furnished by the decom- position of water, 28. P. Perennial plants, how nourished, 46. Peroxide of hydrogen, 63. Petersen and Schd'dler, their analysis of woods, 19. Phosphates necessary to plants, 53. Phosphate of iron, the probable cause of rust, 75. Phosphoric acid in ashes of plants, 53 Source of, 53. Physiologists, their experiments not satisfactory, 22 Neglect of chemistry by, 20. Pipe-clay, ammonia in, 35. Plants absorb oxygen, 18 Ashes of, salts in, 37 Conditions necessary for their life, 22 De- cay of, a source of oxygen, 29 Decompose carbonic acid, 16 Developement of, requisites for, 11, 40, 46, 48 Effect of, on rocks, 51 Elements of, 10 Emit acetic acid, 51 Exha- lation of carbonic acid from, 19 Of a former world, 27 Formation of their components, 29 Functions of, 16 Improve the air, 17 Influence of gases on, 18 of shade, 18 In- organic constituents of, 36 Life of, connected with that of animals, 9 Milky-juiced, in bar- ren soils, 27 Organic acids in, 11, 36 salts in, 37 Perennial, nourished, 46- Products of, vary, 47 Size of, proportioned to organs of nourishment, 24 Succession of, its advantage, 55 Vital processes of, 29 Wild, obtain nitrogen from the air, 34 Yield oxygen, 17. Platinum does not decompose nitric acid, 88. Ploughing, its use, 44. Poisons generated by disease, 115 Inorganic, 117 Peculiar class of, 119 Rendered inert by heat, 121. Poisoning, supeificial, 118 By sausages, 120. Pompeii, air from, 15 Bones from, 53. Potash, action of, upon mould, 112 In grapes, 38 Ley of, its effects on excrements, 34 Presence of, in plants, accounted for, 50 Re- placed by soda, 38 Required by plants, 22 Quantity in soils, 50 Silicate of, in soils, 22 Sources of, 50. Potatoes, oil of, 104 Effect of, as food, 47 Ger- mination of, 45 Produce of, increased, 45. Products of transformations, 25. Pus, globules in, 124. Purgative effect of salts explained, 117. Pusey, Mr., on nitrate of soda, 69. Putrefaction, 23, 90 Of animals, 59 Commu- nicated, 121. Putrefaction, source of ammonia, 30 of carbonic acid, 34. Putrefying sausages, death from, 120 their mods of action, 120 Substances, their effect on wounds, 121 alkaline, 123 acid, 123. R. Rain-water, alkali extracted by, 51. Reduction of oxides, 89. Reeds and canes require silica, 53. Removal of branches, effects of, 45 Reservoirs of dung, 64. Rhine, soils in its vicinity, 57 Wines, 105. Ripening of fruit, 45. Root secretions, 55. Roots absorb, 36 Emit extractive matter, 55 Their office, 43. Rotation of crops, 54-59. S. Saliculite of potash, 99. Saline plants, 40. Salsola kali, 38. Salt, volatilisation of, 43. Salts, absorption of, 39 Effect of, on the or- ganism, 116 Effect of, on flesh, 116 on the stomach, 116 Organic, in plants, 11 in tha blood, 1 16 Passage of, through the lungs, 116. Salt-works, loss in, 42. Saltwort, 41. Sand, plants in, 27. Sandy soil, decay of wood in, 111. Saturation, capacity of, 36. Sausages, poisonous, 120. Saussure, his experiments on air, 15 On the growth of plants, 53. Schubler, his observations on rain, 31. Sea-water, analysis of, 42 Contains carbon, 10 Contains ammonia, 42. Silica in grasses, 53 In reeds and canes, 53. Silicate of potash in plants, 22 As a manure, 63, 72. Silver, carbonate of, action on organic acids, 89 Salts, poisonous effects of, 118. Sinapis alba, 128. Size of plants proportional to organs of nourish- ment, 24. Smell, what, 106. Snow-water, ammonia in, 32. Soda may replace potash, 38. Soils, advantage of loosening, 53, 70 Analysis of, 70 Best for meadow-land, 40 Carbon restored to, 26 Chemical nature of its influ- ence, 57 Constituents of, 70-84 Exhaustion of, 51 Ferruginous, improved, 44 Fertile, INDEX. 135 contain phosphoric acid, potash, &c., 82, 83 Fertile, of Vesuvius, 51 From lava, 51 Im- bibe ammonia, 54 Improved by crops, 54 Impoverished by crops, 54 Various kinds of, 70, 53. Stagnant water, effect of, 44. Stalactites in caverns, 43. Starch, accumulation of, in plants, 45 Compo- sition of, 29 Developement of plants influ- enced by, 45 Effect of, on malt, 26 Product of, the life of plants, 18 In willows, 45. Staunton, Sir G., on Chinese manure, 65. Straw, analysis of, 14. Struve, experiments of, 51. Substitution of bases, 37. Sussinic acid, 112. Sugar, action of alkalies upon, 92 acids upon, 92 Composition of, 95 Carbon in sugar, 14 Contained in the maple-tree, 32 In clero- dendron fragrans, &c., 47 Developement of plants, influence on, 45 Fermentation of, 95 In beet-roots, 32 Metamorphosis of, 95 Organic compounds, all form sugar, 91 Pro- duct of the life of plants, 18 Transformation of, 93 When produced, 24. Sulphur, crystallised, diamorphous, 90. Sulphuric acid, action of, on soils, 70, 84. Sulphurous acid arrests decay, 111. Swine, urine of, 68. Synaptas, 128. T. Tabasheer, 58. Tables of English and Hessian weights, 130. Tannic acid, 29. Tartaric acid, 29 Converted into sugar, 29 In wine, 105. Teak tree, salts found in, 53. Teltowa parsnep, 24, 47. Thenard, his experiments on yeast, 95. Tin, action on nitric acid, 58. Tobacco, juice contains ammonia. 53 Leaves of, 106 Nitric acid in, 64 In Virginia, 51 Va- lue of, proportional to the quantity of potash in the soil, 72. Transformation, by heat, 92 Chemical, 25, 87 Chemical transformations differ from decom- positions, 25 Of acetic acid, 92 Of arrago- nite, 90 Of carbonic acid, 48 Of meeonic acid, 92 Not affected by the vital principle, 26 Explained, 26 Of bodies containing ni- trogen, 92 Of bodies destitute of nitrogen, 93 Results of, 26 Of wood, 93 Of cyanic acid, 94 Of cyanogen, 94 Of gluten, 104. Transplantation, effect of, 45. Trees, diseases of, 47 Require alkalies, 52. U. Ulmin, 12. Urea, converted into carbonate of ammonia, 33 In wine, 64. Uric acid, yields ammonia, 64 Transformations of, 64. Urinary calculi, treatment of, 26 Organs, elimi- nate nitrogen, 26. Urine, contains nitrogen, 33 Its use as manure, 68, 71 Of men, &c., 64 Of horses, 68 Human, analysis of, 64 Of cows, 68 Its use in China and Flanders, 33, 65 Of swine, 68. V. Vaccination, its effect, 126. Vegetable albumen, 33 Mould, 112 Juices, fer- mentation of, 95. Vesuvius, fertile soil of, 51. Vines, new mode of manuring, 86 Juice of, yields ammonia, 33. Vinous fermentation, 103. Virginia, early products of its soils, 51. Virus, of small pox, 126 Vaccine, 126. Vitality, what, 21. Vital principle, 26 Value of the term, 26 How balanced in the blood, 122. Vital processes of plants, 56. W. Water, carbonic acid of, absorbed, 16 Composi- tion of, 28 Dissolves mould, 112 Plants, their action upon, 20 Rain, contains ammo- nia, 31 required by plants, 11 required by gypsum, 35 Salt, analysis of, 42. Wavellite, 53. Wheat, analysis of, 53 Ashes of, used as a ma- nure, 72 Exhausts, 52 Gluten of, 33 Why it does not thrive on certain soils, 52 la Vir- ginia, 51. Willows, growth of, 45. Wine, effect of gluten upon, 106 Fermentation of, 106 Properties of, 106 Substances in, 104 Taste and smell, 105 Varieties of, 105. Woad, decomposition of, 97. Wood, charcoal may replace humus, 27 a ma- nure, 87 Decayed combustion of, 112 Ab- sorbs ammonia, 35 Analysis of, 19 Conver- sion of, into humus, 110 Decay of, 110 Requires air, 110 Decomposition of, 87, 97 Effect of moisture and air on, 110 Elements of, 110 Formation of, 47 Source of its car. bon, 14 Transformation of, 93. Wood coal, how produced, 113 Analysis of, 114, 115. Woody fibre, changes in, 110 Composition of, 110 Decomposition of, 110 Difference be- tween it and wood, 110 Formation of, 18 Moist, evolves carbonic acid, 110 Mould from, 113. Wormwood, effect of its culture, 41. Wort, fermentation of, 107. Wounds, effect of putrefying substances on, 120. Y. Yeast, 96 Destroyed, 1 04 Experiments on, 96 Formed, 104 Its mode of action, 97 Its production, 119 Two kinds of, 107. Zinc, decomposition of water with, 29 ANIMAL CHEMISTRY, OR ORGANIC CHEMISTRY IN ITS APPLICATIONS TO PHYSIOLOGY AND PATHOLOGY, BY JUSTUS LIEBIG, M. D., PH. D. F. R. S., M. R. I. A., PROFESSOR OF CHEMISTRY IN THE UNIVERSITY OF OIESSEN. EDITED FROM THE AUTHOR'S MANUSCRIPT, BY WILLIAM GREGORY, M. D., F. R. S. E., M. R. I. A. PR9FESSOE OF MEDICINE AND CHEMISTRY IN THE UNIVERSITY AND KING*S COLLEGE, ABERDEEN. T. B. PETERSON, No. 98 CHESNUT STREET. TO THE BRITISH ASSOCIATION FOR THE ADVANCEMENT OF SCIENCE. AT the meeting of the British Association in Glasgow, in 1840, 1 had the honour to present the first part of a report on the then present state of Organic Chemistry, in which I endeavoured to develope the doctrines of this science in their bearing on Agriculture and Physiology. It affords me now much gratification to be able to communicate to the meeting of the Association for the present year the second part of my labours ; in which I have attempted to trace the application of Organic Chemistry to Animal Physiology and Pathology. In the present work an extensive series of phenomena have been treated in their chemical relations ; and although it would be presumptuous to consider the questions here raised as being definitely resolved, yet those who are familiar with chemistry will perceive that the only method which can lead to their final resolu- tion, namely, the quantitative method, has been employed. The formulae and equations in the second part, therefore, although they are not to be viewed as ascertained truths, and as furnishing a complete, or the only ex- planation of the vital processes there treated of, are yet true in this sense : that being deduced from facts by logical induction, they must stand as long as no new facts shall be opposed to them. When the chemist shows, for example, that the elements of the bile, added to those of the urate of ammonia, correspond exactly to those of blood, he presents to us a fact which is independent of all hypothesis. It remains for the physiolo- gist to determine, by experiment, whether the conclusions drawn by the chemist from such a fact be accurate or erroneous. And whether this question be answered in the affirmative or in the negative, the fact remains, and will some day find its true explanation. I have now to perform the agreeable duty of expressing my sense of the services rendered to me in the preparation of the English edition by my friend, Dr. Gregory. The distinguished station he occupies as a chemist ; the regular education which he has received in the various branches of medicine ; and his intimate acquaintance with the German language all these, taken together, are the best securities that the translation is such as to convey the exact sense of the original ; securities, such as are not often united in the same individual. It is my intention to follow this second part with a third, the completion of which, however, cannot be looked for before the lapse of two years. This third part will contain an investigation of the food of man and animals, the analysis of all articles of diet, and the study of the changes which the raw food undergoes in its preparation ; as, for example, in fermentation (bread,) baking, roasting, boiling, &c. Already, it is true, many analyses have been made for the proposed work ; but the number of objects of investigation is exceedingly large, and in order to determine with accuracy the absolute value of seed, or of flour, or of a species of fodder, &c., as food, the ultimate analysis alone is not sufficient; 'there are required comparative investigations, which present very great difficulties. DR. JUSTUS LIEBIG. Giessen. 3d June, 1842. (3) NOTE. J WOULD beg leave to refer the chemical as well as the physiological reader par- ticularly to the analyses (in Note (27) Appendix) of the animal tissues, which ought to have been referred to on pages 21 and 42, and which at present are only referred to in Note (7.) Since the work was printed, moreover, there has been added, at the end of the Appendix, an interesting paper by Keller (see page 101,) confirming the very important observation of A. Ure, junior, as to the conversion of benzoic acid into hippuric acid in the human body ; a fact which, I perceive, by the Philosophical Magazine for June, has also been confirmed by Mr. Garrod, probably at an earlier period than by M. Keller. The reader will perceive that this fact strengthens materially the argument of the Author on the action of remedies. W. G. PREFACE. BY the application to chemistry of the methods which had for centuries been followed by .philosophers in ascertaining the causes of natural phenomena in physics by the observation of weight and measure LAVOISIER laid the founda- tion of a new science, which, having been cultivated by a host of distinguished men, has, in a singularly short period, reached a high degree of perfection. It was the investigation and determination of all the conditions which are essen- tial to an observation or an experiment, and the discovery of the true principles of scientific research, that protected chemists from error, and conducted them, by a way equally simple and secure, to discoveries which have shed a brilliant light on those natural phenomena which were previously the most obscure and incompre- hensible. The most useful applications to the arts, to industry, and to all branches of knowledge related to chemistry, sprung from the laws thus established ; and this influence was not delayed till chemistry had attained its highest perfection, but came into action with each new observation. All existing experience and observation in other departments of science reacted, in like manner, on the improvement and development of chemistry ; so that chemistry received from metallurgy and from other industrial arts as much benefit as she had conferred on them. While they simultaneously increased in wealth, they mutually contributed to the development of each other. After mineral chemistry had gradually attained its present state of development, the labours of chemists took a new direction. From the study of the constituent parts of vegetables and animals, new and altered views have arisen; and the present work is an attempt to apply these views to physiology and pathology. In earlier times the attempt has been made, and often with great success, to apply to the objects of the medical art the views derived from an acquaintance with chemical observations. Indeed, the great physicians, who lived towards the end of the seventeenth century, were the founders of chemistry, and in those days the only philosophers acquainted with it. The phlogistic system was the dawn of a new day ; it was the victory of philosophy over the rudest empiricism. With all its discoveries, modern chemistry has performed but slender services to physiology and pathology ; and we cannot be deceived as to the cause of this failure, if we reflect that it was found impossible to trace any sort of relation be- tween the observations made in inorganic chemistry, the knowledge of the charac- ters of the elementary bodies and of such of their compounds as could be formed in the laboratory, on the one hand, and the living body, with the characters of its constituents, on the other. Physiology took no share in the advancement of chemistry, because for a long period she received from the latter science no assistance in her own development This state of matters has been entirely changed within five and twenty years. But during this period physiology has also acquired new ways and methods of investi- gation within her own province ; and it is only the exhaustion of these sources ol A2 5 VI PREFACE discovery which has enabled us to look forward to a change in the direction of the labours of physiologists. The time for such a change is now at hand ; and a per- severance in the methods lately followed in physiology would now, from the want, which must soon be felt, of fresh points of departure for researches, render phy- siology more extensive, but neither more profound nor more solid. No one will venture to maintain that the knowledge of the forms and of the phenomena of motion in organized beings is either unnecessary or unprofitable. On the contrary, this knowledge must be considered as altogether indispensable to that of the vital processes. But it embraces only one class of the conditions necessary for the acquisition of that knowledge, and is not of itself sufficient to enable us to attain it. The study of the uses and functions of the different organs, and of their mutual connection in the animal body, was formerly the chief object of physiological re- searches ; but lately this study has fallen into the back-ground. The greater part of all the modern discoveries has served to enrich comparative anatomy far more than physiology. These researches have yielded the most valuable results in relation to the recog- nition of the dissimilar forms and conditions to be found in the healthy and in the diseased organism ; but they have yielded no conclusions calculated to give us a more profound insight into the essence of the vital processes. The most exact anatomical knowledge of the structure of the tissues cannot teach us their uses ; and from the microscopical examination of the most minute reticulations of the vessels we can learn no more as to their functions than we have learned concerning vision fpom counting the surfaces on the eye of the fly. The most beautiful and elevated problem for the human intellect, the discovery of the laws of vitality, cannot be resolved, nay, cannot even be imagined, without an accurate knowledge of chemical forces ; of those forces which do not act at sensi- ble distances ; which are manifested in the same way as those ultimate causes by which the vital phenomena are determined ; and which are invariably found active, whenever dissimilar substances come into contact. Physiology, even in the present day, still endeavours, but always after the fashion of the phlogistic chemists (that is, by the qualitative method,) to apply chemical experience to the removal of diseased conditions; but with all these countless experiments we are not one step nearer to the causes and the essence of disease. With proposing well-defined questions, experimenters have placed blood, urine, and all the constituents of the healthy or diseased frame, in contact with acids, alkalies, and all sorts of chemical re-agents ; and have drawn, from observation of the changes thus produced, conclusions as to their behaviour in the body. By pursuing this method, useful remedies or modes of treatment might by acci- dent be discovered ; but a rational physiology cannot be founded on mere re-actions, and the living body cannot be viewed as a chemical laboratory. In certain diseased conditions, in which the blood acquires a viscid consistence, this state cannot be permanently removed by a chemical action on the fluid circu- lating in the blood-vessels. The deposit of a sediment from the urine may perhaps, be prevented by alkalies, while their action has not the remotest tendency to remove the cause of disease. Again, when we observe, in typhus, insoluble salts of ammonia in the faeces, and a change in the globules of the blood similar to that which may be artificially produced by ammonia, we are not, on that account, PREFACE. Vll entitled to consider the presence of ammonia in the body as the cause, but only as the effect of a cause. Thus medicine, after the fashion of the Aristotelian philosophy, has formed certain conceptions in regard to nutrition and sanguification ; articles of diet have been divided into nutritious and non-nutritious ; but these theories, being founded on observations destitute of the conditions most essential to the drawing of just conclusions, could not be received as expressions of the truth. How clear are now to us the relations of the different articles of food to the objects which they serve in the body, since organic chemistry has applied to the investigation her quantitative method of research ! When a lean goose, weighing 4 Ibs., gains, in thirty- six days, during which it has been fed with 24 Ibs. of maize, 5 Ibs. in weight and yields 3J Ibs. of pure fat, this fat cannot have been contained in the food, ready formed, because maize does not contain the thousandth part of its weight of fat, or of any substance resembling fat. And when a certain number of bees, the weight of which is exactly known, being fed with pure honey, devoid of wax, yield one part of wax for every twenty parts of honey consumed, without any change being perceptible in their health or in their weight, it is impossible any longer to entertain doubt as to the formation of fat from sugar in the animal body. We must adopt the method which has thus led to the discovery of the origin of fat, in the investigation of the origin and alteration of the secretions, as well as in the study of all the other phenomena of the animal body. From the moment that we begin to look earnestly and conscientiously for the true answers to our ques- tions, that we take the trouble, by means of weight and measure, to fix our obser- vations, and express them in the form of equations, these answers are obtained without difficulty. However numerous our observations may be, yet, if they only bear on one side of a question, they will never enable us to penetrate the essence of a natural phe- nomenon in its full significance. If we are to derive any advantage from them, they must be directed to a well defined object ; and there must be an organized connection between them. Mechanical philosophers and chemists justly ascribe to their methods of research the greater part of the success which has attended their labours. The result of every such investigation, if it bear in any degree the stamp of perfection, may always be given in few words; but these few words are eternal truths, to the discovery of which numberless experiments and questions were essential. The researches themselves, the laborious experiments and complicated apparatus, are forgotten as soon as the truth is ascertained. They were the ladders, the shafts, the tools, which were indispensable to enable us to attain to the rich vein of ore ; they were the pillars and air passages which protected the mine from water and from foul air. Every chemical or physical investigation, however insignificant, which lays claim to attention, must in the present day possess this character. From a certaia number of observations it must enable us to draw some conclusion, whether it be extended or limited. The imperfection of the method or system of research adopted by physiologists can alone explain the fact, that for the last fifty years they have established so few new and solid truths in regard to a more profound knowledge of the functions of the most important organs, of the spleen, cf the liver, and of the numerous glands viii PREFACE. of the body ; and the limited acquaintance of physiologists with the methods of research employed in chemistry will continue to be the chief impediment to the progress of physiology, as well as a reproach which that science cannot escape, Before the time of Lavoisier, Scheele, and Priestley, chemistry was not more closely related to physics than she is now to physiology. At the present day chemistry is so fused, as it were, into physics, that it would be a difficult matter to draw the line between them distinctly. The connection between chemistry and physiology is the same, and in another half century it will be found impossible to separate them. Our questions and our experiments intersect in numberless curved lines the straight line that leads to truth. It is the points of intersection that indicate to us the true direction ; but, owing to the imperfection of the human intellect, these curve lines must be pursued. Observers in chemistry and physics have the eye ever fixed on the object which they seek to attain. One may succeed, for a space, in following the direct line; but all are prepared for circuitous paths. Never doubting of the ultimate success of their efforts, provided they exhibit constancy and perseverance, their eagerness and courage are only exalted by difficulties. Detached observations, without connection, are points scattered over the plain, which do not allow us to choose a decided path. For centuries chemistry pre- sented nothing but these points, and sufficient means were available to fill up the intervals between them. But permanent discoveries and real progress were only made when chemists ceased to make use of fancy to connect them. My object in the present work has been to direct attention to the point* of inter- section of chemistry with physiology, and to point out those parts in which the sciences become, as it were, mixed up together. It contains a collection of problems, such as chemistry at present requires to be resolved ; and a number of conclusions drawn according to the rules of that science from such observations as have been made. These questions and problems will be resolved : and we cannot doubt that we shall have in that case a new physiology and a rational pathology. Our sounding line, indeed, is not long enough to measure the depths of the sea, but is not oil that account less valuable to us : if it assist us, in the mean time, to avoid rocks and shoals, its use is sufficiently obvious. In the hands of the physiologist, organic chemistry must become an intellectual instrument, by means of which he will be enabled to trace the causes of phenomena invisible to the bodily sight ; and if among the results which I have developed or indicated in this work, one alone shall admit of a useful application, I shall consider the object for which it was written as fully attained. The path which has led to it will open up other paths ; and this I consider as the most important object to be gained. JUSTUS LIEBIG. Giessen, April, 1842. CONTENTS. PART I. Page 11 Vital force, vis vitae, or vitality . Distinction between animal and vegeta- ble life 11 Assimilation the result of chemical forces 12 Vitality independent of consciousness . 12 Laws of the vital force . . .13 Conditions of animal life . . .13 Nutrition depends on chemical changes 13 Amount of oxygen inspired by an adult man 14 It combines with carbon and hydrogen in the body . . . . .14 The consumption of oxygen varies . 14 Effect of heat on these variations. . 15 The mutual action of oxygen and car- bon in the body is the true source of animal heat 15 The amount of oxygen regulates that of food 16 Effects of climate on the appetite . 16 The process of starvation . . .17 Cause of death in starvation and chro- nic diseases 17 Nerves and muscles not the source of animal body . . . . .18 Amount of animal heat . .19 Nervous and vegetative life . . . 20 Nutrition depends on the constituents of blood 21 Identity of organic composition infibrine and albumen 21 Nutrition in the carnivora the most simple 22 In the herbivora, depends on the azo- tized products of vegetables . . 22 These products identical with the con- stituents of blood .... 22 The blood of animals is therefore formed by vegetables 23 Uses of the non-azotized ingredients of food 23 Changes of the food in the organism of carnivora 24 Carbon accumulates in the bile . . 25 Nitrogen in the urine . . . .25 Page The carbon is consumed or burned . 26 True function of the bile . . .26 Amount of bile secreted . . .27 Assimilation more energetic in the young animal 27 The butter, sugar, &c., of its food sup- port respiration . . . .28 The same is true of the class of herbivora 28 Waste of matter very rapid in carnivora 30 Importance of agriculture to population 30 Assimilation less energetic in the carni- vora 31 Origin of fat in domesticated animals . 31 Its formation is a source of oxygen . 32 It is formed when oxygen is deficient, and is a source of animal heat . . 33 Elements of nutrition and of respiration 35 Gelatine incapable of serving for nutri- tion, strictly so called . . .35 But it may serve to nourish the gelati- nous tissues 35 PART II. THE METAMORPHOSIS OF TISSUES. Discovery of proteine . . . .36 It is formed by vegetables alone . . 37 Theory of chymification . . .37 Use of the saliva . . . . .38 Source of the nitrogen exhaled from the lungs and skin . . . .39 Composition of proteine . . .41 Composition of the animal tissues . 42 Gelatine contains no proteine, although formed from it 42 The secretions contain all the elements of the blood 43 Formula of blood and metamorphoses ofbile 44 Metamorphoses of blood and flesh . 44 The constituents of the urine derived from the metamorphosed tissues . 45 Relation of blood or flesh and proteine to the secretions and excretions . 45 Formation of gelatine . . . ,46 Origin of bile in the carnivora . 47 Origin of bile in the herbivora . . 47 ix CONTENTS. Page Origin of hippuric acid . . .48 Formation of the chief secretions and excretions ..... 48 Soda essential to the bile . . .49 Relation of urine to bile . . .50 Relation of starch to bile . . .51 Uses of common salt . . . .52 Certain remedies take a share in the vital transformations . . .54 Chief qualities of the blood . . .54 Modus operandi of organic remedies . 55 All organic poisons contain nitrogen . 56 Theine identical with caffeine . . 56 Relation of theine and caffeine to bile . 56 Theory of their action . . . .57 Theory of the action of the vegetable alkalies 57 Composition and origin of nervous matter ..... .57 It is re.ated to that of the vegetable al- kalies . . . . .58 Theory of the action of the latter . , 59 Page Phosphorus seems essential tp nervous matter . . ' . . . .59 PART III. 1. The phenomena of motion in the animal organism . . . .60 2. The same subject, with particular reference to the waste and supply or change of matter . . . .69 3. Theory of disease . . . .74 4. Theory of respiration . . .77 APPENDIX. Containing the analytical evidence re- ferred to in the sections in which are described the chemical processes of respiration, nutrition, and the meta- morphosis of tissues . . 80 On the conversion of benzoic acid into hippuric acid in the human body, by W. Keller . 101 INDEX . 103 ORGANIC CHEMISTRY APPLIED TO PHYSIOLOGY AND PATHOLOGY. I. IN the animal ovum., as well as in the seed of a plant, we recognise a certain re- markable force, the source of growth, or in- crease in the mass, and of reproduction, or of supply of the matter consumed ; a force in a state of rest. By the action of external influences, by impregnation, by the pre- sence of air and moisture, the condition of static equilibrium of this force is disturbed ; entering into a state of motion or activity, it exhibits itself in the production of a series of forms, which, although occasionally bounded by right lines, are yet widely dis- tinct from geometrical forms, such as we ob- serve in crystallised minerals. This force is called the vital force, or viz vitce vitality. The increase of mass in a plant is deter- mined by the occurrence of a decomposition which takes place in certain parts of the plant under the influence of light and heat. In the vital process, as it goes on in vegetables, it is exclusively inorganic matter which undergoes this decomposition; and if, with the most distinguished mineralo- gists, we consider atmospherical air and certain other gases as minerals, it may be said that the vital process in vegetables ac- complishes the transformation of mineral substances into an organism endued with life ; that the mineral becomes part of an organ possessing vital force. The increase of mass in a living plant implies that certain component parts of its nourishment become component parts of the plant; and a comparison of the chemical composition of the plant with that of its nourishment, makes known to us, with positive certainty, which of the component parts of the latter have been assimilated, and which have been rejected. The observations of vegetable physiolo- gists and the researches of chemists have mutually contributed to establish the fact, that the growth and development of vege- tables depend on the elimination of oxygen, which is separated from the other compo- nent parts of their nourishment. In contradiction to vegetable life, the life of animals exhibits itself in the continual absorption of the oxygen of the air, and its combination with certain component parts of the anima^ body While no part of an organized being can serve as food to vegetables, until, by the processes of putrefaction and decay, it has assumed the form of inorganic matter, the animal organism requires, for its support and development, highly organized atoms. The food of all animals, in all circum- stances, consists of parts of organisms. Animals are distinguished from vegeta- bles by the faculty of locomotion, and, in general, by the possession of senses. The existence and activity of these dis- tinguishing faculties depend on certain in- struments which are never found in vegeta- bles. Comparative anatomy shows, that the phenomena of motion and sensation de- pend on certain kinds of apparatus, which have no other relation to each other than this, that they meet in a common centre. The substance of the spinal marrow, the nerves, and the fyrain, is in its composition, and in its chemical characters, essentially distinct from that of which cellular sub- stance, membranes, muscles, and skin are composed. Every thing in the animal organism, to which the name of motion can be applied, proceeds from the nervous apparatus. The phenomena of motion in vegetables, the circulation of the sap, for example, observed in many of the characeae, and the closing ot flowers and leaves, depend on physical and mechanical causes. A plant is destitute of nerves. Heat and light are the remote causes of motion in vegetables ; but in ani- mals we recognise in the nervous apparatus a source of power, capable of renewing itself at every moment of their existence. While the assimilation of food in vegeta- bles, and the whole process of their forma- tion, are dependant on certain external in - fluences which produce motion, the deve lopment of the animal organism is, to a certain extent, independent of these external influences, just because the animal body can produce within itself that source of mo- tion which is indispensable to the vital pro- cess. Assimilation, or the process of formation and growth in other words, the passage of matter from a staf> of motion to that of rest goes on in the same way in animals and 12 ANIMAL CHEMISTRY. m vegetables. In both, the same cause de- termines the" increase of mass. This con- stitutes the true vegetative life, which is carried on without consciousness. The activity of vegetative life manifests itself, in vegetables, with the aid of external influences ; in animals, by means of in- fluences produced within their organism. Digestion, circulation, secretion, are no doubt under the influence of the nervous system ; but the force which gives to the germ, tiie leaf, and the radical fibres of the vegetable the same wonderful properties, is the same as that residing in the secreting membranes and glands of animals, and which enables every animal organ to per- form its own proper function. It is only the source of motion that differs in the two great classes of organized beings. While the organs of the vital motions are never wanting in the lowest orders of ani- mals, as in the impregnated germ of the ovum, in which they are developed first of all, we find, in the higher orders of animals, peculiar organs of feeling and sensation, of consciousness and of a higher intellectual existence. Pathology informs us that the true vege- tative life is in no way dependant on the presence of this apparatus ; that the process of nutrition proceeds in those parts of the body where the nerves of sensation and voluntary motion are paralysed, exactly in the same way as in other parts where these nerves are in the normal condition ; and, on the other hand, that the most eiiergetic voli- tion is incapable of exerting any influence on the contractions of the heart, on the mo- tion of the intestines, or on the processes of secretion. The higher phenomena of mental exist- ence cannot, in the present state of science, be referred to their proximate, and still less to their ultimate causes. We only know of them, that they exist ; we ascribe them to an immaterial agency, and that, in so far as its manifestations are connected with matter, an agency entirely distinct from the vital force, with which it has nothing in common. It cannot be denied that this peculiar force exercises a certain influence on the activity of vegetative life, just as other immaterial agents, such as Light, Heat, Electricity, and Magnetism do ; but this influence is not of a determinative kind, and manifests itself only as an acceleration, a retarding, or a dis- turbance of the process of vegetative life. In a manner exactly analogous, the vegetative life re-acts on the conscious mental existence. There are thus two forces which are found in activity together; but consciousness and intellect may be absent in animals as they are in living vegetables, without their vitality being otherwise affected than by the want of a peculiar source of increased energy or of disturbance. Except in regard to this, all the vital chemical processes go on pre- cisely in the same way in man and in the lower animals. The efforts of philosophers, constantly re newed, to penetrate the relations of the soul to animal life, have all along retarded the progress of physiology. In this attempt men left the province of philosophical re- search for that of fancy ; physiologists, car- ried away by imagination, were far from, being acquainted with the laws of purely animal life. None of them had a clear con- ception of the process of development and nutrition, or of the true cause of death. They professed to explain the most obscure psychological phenomena, and yet they were unable to say what fever is, and in what way quinine acts in curing it. For the purpose of investigating the laws of vital motion in the animal body, only one condition, namely, the knowledge of the apparatus which serves for its production, was ascertained; but the substance of the organs, the changes which food undergoes in the living body, its transformation into portions of organs, and its reconversion into lifeless compounds, the share which the at- mosphere takes in the processes of vitality; all these foundations for future conclusions were still wanting. What has the soul, what have conscious- ness and intellect to do with the develop- ment of the human foetus, or the foetus in a fowl's egg? not more, surely, than with the development of the seeds of a plant. Let us first endeavour to refer to their ultimate causes those phenomena of life which are not physiological; and let us beware of drawing conclusions before we have a groundwork. We know exactly the me- chanism of the eye ; but neither anatomy nor chemistry will ever explain how the rays of light act on consciousness, so as to produce vision. Natural science has fixed limits which cannot be passed ; and it must always be borne in mind that, with all our discoveries, we shall never know what light, electricity, and magnetism are in their es- sence, because, even of those things which are material, the human intellect has only conceptions. We can ascertain, however, the laws which regulate their motion and rest, because these are manifested in pheno- mena. In like manner the laws of vitality, and of all that disturbs, promotes, or alters it, may certainly be discovered, although we shall never learn what life is. Thus the discovery of the laws of gravitation and of the planetary motions led to an entirely new conception of the cause of these phenomena. This conception could not have been formed in all its clearness without a knowledge of phenomena out of which it was evolved ; for, considered by itself, gravity, like light to one born blind^ is a mere word, devoid of meaning. The modern science of physiology has left the track of Aristotle. To the eternal advantage of science, and to the benefit of mankind, it no longer invents a horror vacui, a quinta essentia, in order to furnish credu- lous hearers with solutions and explanations CHEMICAL CHANGES. 13 of phenomena, whose true connection with others, whose ultimate cause is still un- known. If we assume that all the phenomena ex- hibited by the organism of plants and ani- mals are to be ascribed to a peculiar cause, different in its manifestations from all other causes which produce motion or change of condition; if, therefore, we regard the vital force as an independent force, then, in the phenomena of organic life, as in all other phenomena ascribed to the action of forces, we have the statics, that is, the state of equi- librium determined by a resistance, and the dynamics, of the vital force. All the parts of the animal body are pro- duced from a peculiar fluid, circulating in its organism, by virtue of an influence resid- ing in every cell, in every organ, or part of an organ. Physiology teaches that all parts of the body were originally blood ; or that at least they were brought to the growing organs by means of this fluid. The most ordinary experience farther shows, that at each moment of life, in the animal organism, a continued change of matter, more or less accelerated, is going on ; that a part of the structure is transformed into unorganized matter, loses its condition of life, and must be again renewed. Physi- ology has sufficiently decisive grounds for the opinion, that every motion, every mani- festation of force, is the result of a transfor- mation of the structure or of its substance; that every conception, every mental affec tion, is followed by changes in the chemical nature of the secreted fluids ; that every thought, every sensation, is accompanied by a change in the composition of the sub- stance of the brain. In order to keep up the phenomena of life in animals, certain matters are required, parts of organisms, which we call nourish- ment. In consequence of a series of altera- tions, they serve either for the increase oi the mass (nutrition,) or for the supply oJ the matter consumed (reproduction,) or, finally, for the production of force. II. If the first condition of animal life be the assimilation of what is commonly callec nourishment, the second is a continual absorption of oxygen from the atmos- phere. Viewed as an oljject of scientific research, animal life exhibits itself in a series of phenomena, the connection and recurrence of which are determined by the changes which the food and the oxygen absorbed from the atmosphere undergo in the organ- ism under the influence of the vital force. All vital activity arises from the mutual action of the oxygen of the atmosphere and the elements of the food. In the processes of nutrition and repro- duction, we perceive the passage of matter from the state of motion to that of rest (static equilibrium ;) under the influence of the nervous system, this matter enters again into a state of motion. The ultimate causes f these different conditions of tne vital force are chemical forces. The cause of the state of rest is a resist- ance, determined by a force of attraction ^combination,) which acts between the smallest particles of matter, and is mani- ested only when these are in actual contact, or at infinitely small distances. To this peculiar kind of attraction we may of course apply different names ; but the chemist calls it affinity. The cause of the state "of motion is to be found in a series of changes which the food undergoes in the organism, and these are the results of processes of decomposition, to which either the food itself, or the structures formed from it, or parts of organs, are sub- jected. The distinguishing character of vegetable life is a continued passage of matter from the state of motion to that of static equili- brium. While a plant lives, we cannot perceive any cessation in its growth; no part of an organ in the plant diminishes in size. If decomposition occur, it is the re- sult of assimilation. A plant produces within itself no cause of motion ; no part of its structure, from any influence residing in its organism, loses its state of vitality, and is converted into unorganized, amor- phous compounds; in a word, no waste occurs in vegetables. Waste, in the animal body, is a change in the state or in the composition of some of its parts, and conse- quently is the result of chemical actions. The influence of poisons and of remedial agents on the living animal body evidently shows that the chemical decompositions and combinations in the body, which manifest themselves in the phenomena of vitality, may be increased in intensity by chemical forces of analogous character, and retarded or put an end to by those of opposite cha- racter; and that we are enabled to exercise an influence on every part of an organ by means of substances possessing a well- defined chemical action. As, in the closed galvanic circuit, in con- sequence of certain changes which an inor- ganic body, a metal, undergoes when placed in contact with an acid, a certain something becomes cognizable by our senses, which we call a current of electricity ; so, in the animal body, in consequence of transforma- tions and changes undergone by matter previously constituting a part of the organ- ism, certain phenomena of motion and activity are perceived, and these we call life, or vitality. The electrical current manifests itself in certain phenomena of attraction and repul- sion, which it excites in other bodies na- turally motionless, and by the phenomena of the formation and decomposition of che- mical compounds, which occur every where, when the resistance is not sufficient to arrest the current. It is from this point of view, and from no other, that chemistry ought to contemplate B 14 ANIMAL CHEMISTRY. the phenomena of life. Wonders surround us on every side. The formation of a crystal, of an octahedron, is not less incom- prehensible than the production of a leaf or of a muscular fibre; and the production of vermilion from mercury and sulphur is as much an enigma as the formation of an eye from the substance of the blood. The first conditions of animal life are nu- tritious matters and oxygen, introduced into the system. At every moment of his life man is taking oxygen into his system, by means of the organs of respiration ; no pause is observ- able while life continues. The observations of physiologists have shown that the body of an adult man, sup- plied with sufficient food, has neither in- creased nor diminished in weight at the end of twenty-four hours ; yet the quantity of oxygen taken into the system during this period is very considerable. According to the experiments of Lavoisier, an adult man takes into his system, from the atmosphere, in one year, 746 Ibs., according to Menzies, 837 Ibs. of oxygen; yet we find his weight, at the beginning and end of the year, either quite the same, or differing, one way or the other, by at most a few pounds. (1.)* What, it may be asked, has become of the enormous weight of oxygen thus intro- duced, in the course of a year into the human system ? This question may be answered satisfac- torily ; no part of this oxygen remains in the system ; but it is given out again in the form of a compound of carbon or of hydrogen. The carbon and hydrogen of certain parts of the body have entered into combination with the oxygen introduced through the lungs and through the skin, and have been given out in the forms of carbonic acid gas and the vapour of water. At every moment, with every expiration, certain quantities of its elements separate from the animal organism, after having en- tered into combination, within the body, with the oxygen of the atmosphere. If we assume, with Lavoisier and Seguin, in order to obtain a foundation for our cal- culation, that an adult man receives into his system daily 32oz. (46,037 cubic inches= 15,661 grains, French weight) of oxygen, and that the weight of the whole mass of his blood, of which 80 per cent, is water, is 24 Ibs. ; it then appears, from the known composition of the blood, that, in order to convert the whole of its carbon and hydro- gen into carbonic acid and water, 64,103 grains of oxygen are required. This quan- tity will be taken into the system of an adult in four days five hours. (2) Whether this oxygen enters into combi- nation with the elements of the blood, or with other parts of the body containing car- bon and hydrogen, in either case the conclu- * The Numbers refer to the Appendix. sion is inevitable, that the body of a man, who daily takes into the system 32 oz. of oxygen, must receive daily in the shape of nourishment, as much carbon and hydrogen as would suffice to supply 24 Ibs. of blood with these elements ; it being presupposed that the weight of the body remains un- changed, and that it retains its normal con- dition as to health. This supply is furnished in the food. From the accurate determination of the quantity of carbon daily taken into the sys- tem in the food, as well as of that propor- tion of it which passes out of the body in the faeces and urine, unburned, that is, in some form in which it is not combined with oxygen, it appears that an adult, taking moderate exercise, consumes 13.9 oz. of carbon daily. (3) These 13^\ oz. of carbon escape through the skin and lungs as carbonic acid gas. For conversion into carbonic acid gas, oz. of carbon require 37 oz. of oxygen. According to the analyses of Boussingault (Ann. de Ch. et de Ph. LXXI. p. 136) a horse consumes in twenty-four hours 97 oz. of carbon, a milk cow 69^ oz. The quantities of carbon here mentioned are those given off from the bodies of these ani- mals in the form of carbonic acid j and it appears from them that the horse consumes, in converting carbon into carbonic acid, 13 Ibs. 3 oz. in twenty-four hours, and the milk cow 11 Ibs. lOf oz. of oxygen in the same time. (4) Since no part of the oxygen taken into the system is again given off in any other form but that of a compound of carbon or hydrogen j since, farther, the carbon and hy- drogen given off are replaced by carbon and hydrogen supplied in the food,, it is clear that the amount of nourishment required by the animal body must be in a direct ratio to the quantity of oxygen taken into the system. Two animals, which in equal times take up by means of the lungs and skin unequal quantities of oxygen, consume quantities of the same nourishment which are unequal in the same ratio. The consumption of oxygen in squal times may be expressed by the number of respirations ; it is clear that, in the same in dividual, the quantity of nourishment re- quired must vary with the force and num- ber of the respirations. A child, in whom the organs of respiration are naturally very active, requires food of- tener than an adult, and bears hunger less easily. A bird, deprived of food, dies on the third day, while a serpent, with its sluggish respiration, can live without food three months and longer. The number of respirations is smaller in a state of rest than during exercise or work. The quantity of food necessary in both con- ditions must vary in the same ratio. An excess of food is incompatible with deficiency In respired oxygen, that is, with SOURCE OF ANIMAL HEAT. RESPIRATION. 15 deficient exercise ; just as violent exercise, which implies an increased supply of food, is incompatible with weak digestive organs. In either case the health suffers. But the quantity of oxygen inspired is also affected by the temperature and density of the atmosphere. The capacity of the chest in an animal is a constant quantity. At every respiration a quantity of air enters, the volume of which may be considered as uniform; but its weight, and consequently that of the oxygen it contains, is not constant. Air is expanded by heat, and contracted by cold, and there- fore equal volumes of hot and cold air con- tain unequal weights of oxygen. In sum- mer, moreover, atmospherical air contains aqueous vapour, while in winter it is dry ; the space occupied by vapour in the warm air is filled up by air itself in winter ; that is, it contains, for the same volume, more oxygen in winter than in summer. In summer and in winter, at the pole and at the equator, we respire an equal vo- lume of air ; the cold air is warmed during respiration, and acquires the temperature of the body. To introduce into the lungs a given volume of oxygen, less expenditure of force is necessary in winter than in sum- mer ; and for the same expenditure of force, more oxygen is inspired in winter. It is oovious, that in an equal number of respirations we consume more oxygen at the level of the sea than on a mount, in. The quantity both of oxygen inspired and of carbonic acid expired, must, therefore, vary with the height of the barometer. The oxygen taken into the system is given out again in the same forms, whether in summer or in winter; hence we expire more carbon in cold weather, and when the barometer is high, than we do in warm weather; and we must consume more or less carbon in our food in the same propor- tion ; in Sweden more than in Sicily ; and in our more temperate climate a full eighth more in winter than in summer. Even when we consume equal weights of food in cold and warm countries, infinite wisdom has so arranged, that the articles of food in different climates are most unequal in the proportion of carbon they contain. The fruits on which the natives of the south prefer to feed do not in the fresh state contain more than 12 per cent, of carbon, while the bacon and train oil used by the inhabitants of the arctic regions contain from 66 to 80 per cent, of carbon. It is no difficult matter, in warm climates, to study moderation in eating, and men can bear hunger for a long time under the equa- tor; but cold and hunger united very soon exhaust the body. The mutual action between the elements of the food and the oxygen conveyed by the circulation of the blood to every part of the body is THE SOURCE OF ANIMAL HEAT. III. All living creatures, whose existence depends on the absorption of oxygen, pos- sess within themselves a source of heat in- dependent of surrounding objects. This truth applies to all animals, and ex- tends, besides, to the germination of seeds, to the flowering of plants, and to the matura- tion of fruits. It is only in those parts of the body to which arterial blood, and with it the oxygen absorbed in respiration, is conveyed, that heat is produced. Hair, wool, or feathers, do not possess an elevated temperature. This high temperature of the animal body, or, as it may be called, disengagement of heat, is uniformly and under all circum- stances the result of the combination of a combustible substance with oxygen. In whatever way carbon may combine with oxygen, the act of combination cannot take place without the disengagement of heat. It is a matter of indifference whether the combination take place rapidly or slowly, at a high or at a low temperature: the amount of heat liberated is a constant quantity. The carbon of the food, which is con- verted into carbonic acid within the body, must give out exactly as much heat as if it had been directly burnt in the air or in oxy- gen gas; the only difference is, that the amount of heat produced is diffused over unequal times. In oxygen, the combustion is more rapid, and the heat more intense ; in air it is slower, the temperature is not so high, but it continues longer. It is obvious that the amount of heat libe- rated must increase or diminish with the quantity of oxygen introduced in equal times by respiration. Those animals which respire frequently, and consequently, con- sume much oxygen, possess a higher tem- perature than others, which, with a body of equal size to be heated, take into the system less oxygen. The temperature of a child (102) is higher than that of an adult (99-50). That of birds (104 to 105-40) is higher than that of quadrupeds (98'5 to 100-4) or than that of fishes or amphibia, whose proper temperature is from 2-7 to 3-6 higher than that of the medium in which they live. All animals, strictly speaking are warm-blooded ; but in those only which possess lungs is the temperature of the body quite independent of the sur- rounding medium. (5) The most trustworthy observations prove that in all climates, in the temperate zones as well as at the equator or the poles, the temperature of the body in man, and in what are commonly called warm-blooded animals, is invariably the same; yet how different are the circumstances under which they live ! The animal body is a heated mass, which bears the same relation to surrounding ob- jects as any other heated mass. It receives heat when the surrounding objects are hotter, it loses heat when they are colder than itself. We know that the rapidity of cooling in 16 ANIMAL CHEMISTRY. creases with the difference between the tem- perature of the heated body and that of the surrounding medium ; that is, the colder the surrounding medium the shorter the time required for the cooling of the heated body. How unequal,, then, must be the loss of heat in a man at Palermo, where the exter- nal temperature is nearly equal to that of the body, and in the polar regions, where the external temperature is from 70 to 90 lower. Yet, notwithstanding this extremely un- equal loss of heat, experience has shown that the blood of the inhabitant of the arctic circle has a temperature as high as that of the native of the south, who lives in so dif- ferent a medium. This fact, when its true significance is per- ceived, proves that the heat given off to the surrounding medium is restored within the body with great rapidity. This compensa- tion takes place more rapidly in winter than in summer, at the pole than at the equator. Now, in different climates the quantity of oxygen introduced into the system of respi- ration, as has been already shown, varies according to the temperature of the exter- nal air ; the quantity of inspired oxygen in- creases with the loss of heat by external cooling, and the quantity of carbon or hydro- gen necessary to combine with this oxygen must be increased in the same ratio. It is evident that the supply of the heat lost by cooling is effected by the mutual action of the elements of the food and the inspired oxygen, which combine together. To make use of a familiar, but not on that account a less just illustration, the animal body acts, in this respect, as a furnace, which we supply with fuel. It signifies nothing what intermediate forms food may assume, what changes it may undergo in the body, the last change is uniformly the con- version of its carbon into carbonic acid, and of its hydrogen into water; the unassimila- ted nitrogen of the food, along with the un- burned or unoxidised carbon, is expelled in the urine or in the solid excrements. In order to keep up in the furnace a constant temperature, we must vary the supply of fuel according to the external temperature, that is, according to the supply of oxygen. In the animal body the food is the fuel ; with a proper supply of oxygen we obtain the heat given out during its oxidation or combustion. In winter, when we take exercise in a cold atmosphere, and when consequently, the amount of inspired oxygen increases, the necessity for food containing carbon and hydrogen increases in the same ratio; and by gratifying the appetite thus excited, we obtain the most efficient protec- tion against the most piercing cold. A starving man is soon frozen to death ; and every one knows that the animals of prey in the arctic regions far exceed in voracity those of the torrid zone. In cold and temperate climates, the air, which incessantly strives to consume the body, urges man to labourious efforts in order to furnish the means of resistance to its action, while, in hot climates, the neces- sity of labour to provide food is far less urgent. Our clothing is merely an equivalent for a certain amount of food. The more warmly we are clothed the less urgent becomes the appetite for food, because the loss of heat by cooling, and consequently the amount of heat to be supplied by the food, is di- minished. If we were to go naked, like certain savage tribes, or if in hunting or fishing we were exposed to the same degree of cold as the Samoyedes, we should be able with ease to consume 10 Ibs. of flesh, and perhaps, a dozen of tallow candles into the bargain, daily, as warmly clad travellers have re- lated with astonishment of these people! We should, then, also be able to take the same quantity of brandy or train oil without bad effects, because the carbon and hydrogen of these substances would only suffice to keep up the equrlibrium between the exter- nal temperature and that of our bodies. According to the preceding expositions, the quantity of food is regulated by the number of respirations, by the temperature of the air, and by the amount of heat given off to the surrounding medium. No isolated fact, apparently opposed to this statement, can affect the truth of this natural law. Without temporary or perma- nent injury to health, the Neapolitan cannot take more carbon and hydrogen in the shape of food than he expires as carbonic acid and water ; and the Esquimaux cannot ex- pire more carbon and hydrogen than he takes into the system as food, unless in a state of disease or of starvation. Let us ex- amine these states a little more closely. The Englishman in Jamaica sees with regret the disappearance of his appetite, previously a source of frequently recurring enjoyment; and he succeeds by the use of cayenne pepper and the most powerful stimulants, in enabling himself to take as much food as he was accustomed to eat at home. But the whole of the carbon thus introduced into the system is not consumed; the temperature of the air is too high, and the oppressive heat does not allow him to increase the number of respirations by active exercise, and thus to proportion the waste to the amount of food taken; disease of some kind, therefore, ensues. On the other hand, England sends her sick, whose diseased digestive organs have in a greater or less degree lost the power of bringing the food into that state in which it is best adapted for oxidation, and therefore, furnish less resistance to the oxidising agency of the atmosphere than is required in their native climate, to southern regions, where the amount of inspired oxygen is diminished in so great a proportion; and the result, an improvement in the health, is obvious. The diseased organs of diges- EFFECTS OF STARVATION. 17 tion have sufficient power to place the di- minished amount of food in equilibrium with the inspired oxygen ; in the colder climate, the organs of respiration them- selves would have been consumed in fur- nishing the necessary resistance to the action of the atmospheric oxygen. In our climate, hepatic diseases, or those arising from excess of carbon, prevail in summer ; in winter, pulmonic diseases, or those arising from excess of oxygen, are more frequent. The cooling of the body, by whatever cause it may be produced, increases the amount of food necessary. The mere ex- posure to the open air, in a carriage or on the deck of a ship, by increasing radiation and vaporization, increases the loss of heat, and compels us to eat more than usual. The same is true of those who are accus- tomed to drink large quantities of cold water, which is given off at the temperature of the body, 98'5. It increases the appe- tite, and persons of weak constitution find it necessary, by continued exercise, to sup- ply to the system the oxygen required to restore the heat abstracted by the cold water. Loud and long continued speaking, the crying of infants, moist air, all exert a decided and appreciable influence on the amount of food which is taken. IV. In the foregoing pages, it has been assumed that it is especially carbon and hydrogen which, by combining with oxy- gen, serve to produce animal heat. In fact, observation proves that the hydrogen of the food plays a not less important part than the carbon. The whole process of respiration appears most clearly developed, when we consider the state of a man, or other animal, totally deprived of food. The first effect of starvation is the disap- pearance of fat, and this fat cannot be traced in the urine or in the scanty fasces. Its car- bon and hydrogen have been given off through the skin and lungs in the form of oxidised products ; it is obvious that they have served to support respiration. In the case of a starving man, 32^ oz. of oxygen enter the system daily, and are given out again in combination with a part of his body. Currie mentions the case of an individual who was unable to swallow, and whose body lost 100 Ibs. in weight dur- ing a month; and, according to Martell (Trans. Linn. Soc., vol. xi. p. 411,) a fat pig, overwhelmed in a slip of earth, lived 160 days without food, and was found to have diminished in weight, in that time, more than 120 Ibs. The whole history of hybernating animals, and the well esta- blished facts of the periodical accumulation, in various animals, of fat, which, at other periods, entirely disappears, prove that the oxygen, in the respiratory process, con- sumes, without exception, all such sub- stances as are capable of entering into combination with it. It combines with whatever js presented to it ; and the defici- ency of hydrogen is the only reason why carbonic acid is the chief product; for, at the temperature of the body, the affinity of hydrogen for oxygen far surpasses that of carbon for the same element. We know, in fact, that the graminivora expire a volume of carbonic acid equal to that of the oxygen inspired, while the carni- vora, the only class of animals whose food contains fat, inspire more oxygen than is equal in volume to the carbonic acid ex- pired. Exact experiments have shown, that in many cases only half the volume of oxygen is expired in the form of carbonic acid. These observations cannot be gain- said, and are far more convincing than those arbitrary and artificially produced pheno- mena, sometimes called experiments ; expe- riments which, made as too often they are, without regard to the necessary and natural conditions, possess no value, and may be entirely dispensed with ; especially when, as in the present case, nature affords the op- portunity for observation, and when we make a rational use of that opportunity. In the progress of starvation, however, it is not only the fat which disappears, but also, by degrees, all such of the solids as are capable of being dissolved. In the wasted bodies of those who have suffered starvation, the muscles are shrunk and un- naturally soft, and have lost their contracti- lity ; all those parts^of the body which were capable of entering into the state of motion, have served to protect the remainder of the frame from the destructive influence of the atmosphere. Towards the end, the parti- cles of the brain begin to undergo the process of oxidation, and delirium, mania, and death close the scene ; that is to say, all resistance to the oxidising power of the atmospheric oxygen ceases, and the chemical process of eremacausis, or decay, commences, in which every part of the body, the bones excepted, enters into combination with oxygen. v/ The time which is required to cause death by starvation depends on the amount of fat in the body, on the degree of exercise, as in labour or exertion of any kind, on the tem- perature of the air, and finally, on the pre- sence or absence of water. Through the skin and lungs there escapes a certain quan- tity of water, and as the presence of water is essential to the continuance of the vital motions, its dissipation hastens death. Cases have occurred, in which a full supply of water being accessible to the sufferer, death has not occurred, till after the lapse of twenty days. In one case, life was sus- tained in this way for the period of sixty days. In all chronic diseases death is produced by the same cause, namely, the chemical action of the atmosphere. When those substances are wanting, whose function in the organism is to support the process of respiration; when the diseased organs ar* incapable of performing their proper funo- B2 18 ANIMAL CHEMISTRY. tion of producing these substances; when they have lost the power of transforming the food into that shape in which it may, by entering into combination with the oxy- gen of the air, protect the system from its influence, then, the substance of the organs themselves, the fat of the body, the sub- stance of the muscles, the nerves, and the brain, are unavoidably consumed.* The true cause of death in these cases is the respiratory process, that is, the action of the atmosphere. A deficiency of food, and the want of power to convert the food into a part of the organism, are both, equally a want of resist- ance ; and this is the negative cause of the cessation of the vital process. The flame is extinguished, because the oil is consumed ; and it is the oxygen of the air which has consumed it. In many diseases substances are produced which are incapable of assimilation. By the mere deprivation of food, these sub- stances are removed from the body without leaving a trace behind ; their elements have entered into combination with the oxygen of the air. From the first moment that the function of the lungs or of the skin is interrupted or disturbed, compounds, rich in carbon, ap- pear in the urine, which acquires a brown colour. Over the whole surface of the body oxygen is absorbed, and combines with all the substances which offer no resistance to it. In those parts of the body where the access of oxygen is impeded ; for example, in the armpits, or in the soles of the feet, peculiar compounds are given out, recog- nisable by their appearance, or by their odour. These compounds contain much carbon. Respiration is the falling weight, the bent spring, which keeps the clock in motion; the inspirations and expirations are the strokes of the pendulum which regulate it. In our ordinary timepieces, we know with mathematical accuracy the effect produced on their rate of going, by changes in the length of the pendulum, or in the external temperature. Few, however, have a clear conception of the influence of air and tem- perature on the health of the human body ; and yet the research into the conditions ne- cessary to keep it in the nominal state, is not more difficult than in the case of a clock. V. The want of a just conception of force and effect, and of the connection of natural phenomena, has led chemists to attribute a part of the heat generated in the animal body to the action of the nervous system. If this view exclude chemical action, or changes in the arrangement of the elemen- tary particles, as a condition of nervous * For an account of what really takes place in this process, I refer to the considerations on the means by which the change of matter is effected in the body of the carnivora, which will be found farther on. agency, it means nothing else than to derive the presence of motion, the manifestation of a force, from nothing. But no force, no power can come of nothing. No one will seriously deny the share which the nervous apparatus has in the respiratory process ; for no change of condi- tion can occur in the body without the nerves ; they are essential to all vital motions. Under their influence, the viscera produce those compounds, which, while they protect the organism from the action of the oxygen of the atmosphere, give rise to animal heat; and when the nerves cease to perform their functions, the whole process of the action of oxygen must assume another form. When the pons Varolii is cut through in the dog, or when a stunning blow is inflicted on the back of the head, the animal continues to respire for some time, often more rapidly than in the nominal state; the frequency of the pulse at first rather increases than diminishes, yet the animal cools as rapidly as if sudden death had occurred. Exactly similar observations have been made on the cutting of the spinal chord, and of the par vagum. The respiratory motions continue for a time, but the oxygen does not meet with those substances with which, in the normal state, it would have combined ; be- cause the paralysed viscera will no longer furnish them. The singular idea that the nerves produce animal heat, has obviously arisen from the notion that the inspired oxy- gen combines with carbon, in the blood itself; in which case the temperature of the body, in the above experiments, certainly, ought not to have sunk. But, as we shall afterwards see, there cannot be a more erro- neous conception than this. As by the division of the pneumogastric nerves the motion of the stomach and the secretion of the gastric juice are arrested, and an immediate check is thus given to the process of digestion, so the paralysis of the organs of vital motion in the abdominal vis- cera affects the process of respiration. These processes are most intimately connected; and every disturbance of the nervous system or of the nerves of digestion re-acts visibly on the process of respiration. The observation has been made, that heat is produced by the contraction of the mus- cles, just as in a piece of caoutchouc, which, when rapidly drawn out, forcibly contracts again, with disengagement of heat. Some have gone so far as to ascribe a part of the animal heat to the mechanical motions of the body, as if these motions could exist without an expenditure of force consumed in producing them ; how then, we may ask, is this force produced ? By the combustion of carbon, by the solu- tion of a metal in an acid, by the combina- tion of the two electricities, positive and negative, by the absorption of light, and even by the rubbing of two solid bodies together with a certain degree of rapidity, heat may be produced. GREAT AMOUNT OP ANIMAL HEAT. 19 By a number of causes, in appearance entirely distinct, we can thus produce one and the same effect. In combustion and in the production of galvanic electricity, we have a change of condition in material par- ticles ; when heat is produced by the ab- sorption of light or by friction, we have the conversion of one kind of motion into an- other, which affects our senses differently. In all such cases we have a something given, which merely takes another form; in all we have a force and its effect. By means of the fire which heats the boiler of a steam engine we can produce every kind of motion, and by certain amount of motion we can produce fire. When we rub a piece of sugar briskly on an iron grater, it undergoes, at the surfaces of contact, the same change as if exposed to heat ; and two pieces of ice, when rubbed together, melt at the point of contact. Let us remember that the most distin- guished authorities in physics consider the phenomena of heat as phenomena of motion, because the very conception of the creation of matter, even though imponderable, is ab- solutely irreconcilable with its production by mechanical causes, such as friction or motion. But, admitting all the influence which electric or magnetic disturbances in the ani- mal body can have on the functions of its organs, still the ultimate cause of all these forces is a change of condition in material particles, which may be expressed by the conversion, within a certain time, of the ele- ments of the food into oxidised products. Such of these elements as do not undergo this process of slow combustion, are given off unburned or incombustible in the exe- crements. Now, it is absolutely impossible that a given amount of carbon or hydrogen, what- ever different forms they may assume in the progress of the combustion, can produce more heat than if directly burned into atmos- pheric air or in oxygen gas. When we kindle a fire under a steam engine, and employ the power obtained to produce heat by friction, it is impossible that the heat thus obtained can ever be greater than that which was required to heat the boiler ; and if we use the galvanic current to produce heat, the amount of heat obtained is never in any circumstances, greater than we might have by the com- bustion of the zinc which has been dissolved in the acid. The contraction of muscles produces heat; but the force necessary for the contraction has manifested itself through the organs of motion, in which it has been excited by chemical changes. The ultimate cause of the heat produced is, therefore, to be found in these chemical changes. By dissolving a metal in an acid, we produce an electrical current ; this current, if passed through a wire, converts the wire into a magnet, by means of which, many different effects may be produced. The cause of this phenomena is magnetism; the cause of the magnetic phenomena is to be found in the electrical current ; and the ulti- mate cause of the electrical current is found to be a chemical change, a chemical action. There are various causes by which force or motion may be produced. A bent spring, a current of air, the fall of water, fire ap- plied to a boiler, the solution of a metal in an acid, all these different causes of mo- tion may be made to produce the same effect. But in the animal body we recog- nise as the ultimate cause of all force only one cause, the chemical action which the elements of the food and the oxygen of the air mutually exercises on each other. The only known ultimate cause of vital force, either in animals or in plants, is a chemical process. If this be prevented, the pheno- mena of life do not manifest themselves, or they cease to be recognisable by our senses. If the chemical action be impeded, the vital phenomena must take new forms. According to the experiments of Despretz, 1 oz. of carbon evolves, during its combus- tion, as much heat as would raise the tem- perature of 105 oz. of water at 32 to 167, that is, by 135 degrees; in all, therefore, 105 times 135=14207 degrees of heat. Consequently, the 13*9 oz. of carbon which are daily converted into carbonic acid in the body of an adult, evolve 13-9x14207= 197477-3 degrees of heat. This amount o/ heat is sufficient to raise the temperature ol 1 oz. of water by that number of degrees,, or from 32 to 197509-3; or to cause 136-8 Ibs. of water at 32 to boil; or to heat 370 Ibs. of water to 98-3 (the tem- perature of the human body ;) or to convert into vapour 24 Ibs. of water at 98-3. If we now assume that the quantity of water vaporized through the skin and lungs in 24 hours amounts to 48 oz. (3 Ibs.,) then there will remain, after deducting the neces- sary amount of heat, 146380-4 degrees of heat, which are dissipated by radiation, by heating the expired air, and in the excre- mentitious matters. In this calculation, no account has been taken of the heat evolved by the hydrogen of the food, during its conversion into water by oxidation within the body. But if we consider that the specific heat of the bones, of fat, and of the organs generally, is far less than that of water, and that conse- quently they require, in order to be heated to 98-3, much less heat than an equal weight of water, no doubt can be enter- tained, that when all the concomitant cir- cumstances are included in the calculation, the heat evolved in the process of combus- tion, to which the food is subjected in the body, is amply sufficient to explain the con- stant temperature of the body, as well as the evaporation from the skin and lungs. VI. All experiments hitherto made on the quantity of oxygen which an animal con- sumes in a given time, and also the conclu- 20 ANIMAL CHEMISTRY. sions deduced from them as the origin of animal heat, are destitute of practical value in regard to this question, since we have seen that the quantity of oxygen consumed varies according to the temperature and density of the air., according to the degree of motion, labour, or exercise, to the amount and quality of food, to the comparative warmth of the clothing, and also according to the time within which the food is taken. Prisoners in the Bridewell at Marienschloss (a prison where labour is enforced,) do not consume more than 1O5 oz. of carbon daily ; those in the House of Arrest at Giessen, who are deprived of all exercise, consume only 8*5 oz. ; (6) and in a family well known to me, consisting of nine individuals, five adults, and four children of different ages, the average daily consumption of carbon for each, is not more than 9'5 oz. of carbon.* We may safely assume, as an approxima- tion, that the quantities of oxygen consumed in these different cases are in the ratio of these numbers ; but where the food contains meat, fat, and wine, the proportions are altered by reason of the hydrogen in these kinds of food which is oxidised,- and which, in being converted into water, evolves much more heat for equal weights. The attempts to ascertain the amount of heat evolved in an animal for a given con- sumption of oxygen have been equally unsatisfactory. Animals have been allowed to respire in close chambers surrounded with cold water; the increase of tempera- ture in the water has been measured by the thermometer, and the quantity of oxygen consumed has been calculated from the analysis of the air before and after the ex- periment. In experiments thus conducted, it has been found that the animal lost about jV more heat than corresponded to the oxygen consumed; and had the windpipe of the animal been tied, the strange result would have been obtained of a rise in the temperature of the water without any con- sumption of oxygen. The animal was at the temperature of 98 or 99, and the water, in the experiments of Despretz, was at 47'5. Such experiments consequently prove, that when a great difference exists between the temperature of the animal body and that of the surrounding medium, and when no motion is allowed, more heat is given off than corresponds to the oxygen consumed. In equal times, with free and unimpeded motion, a much larger quantity of oxygen would be consumed without a perceptible increase ir .he amount of heat lost. The cause of these phenomena is * In this family, the monthly consumption was 151 Ibs. of brown bread, 70 Ibs. white bread, 132 Ibs. meat, 19 Ibs. sugar, 15'9 Ibs. butter, 57 maass (about 24 gallons) ot milk ; the carbon of the po- tatoes and other vegetables, of the poultry, game, nnd wine consumed, having been reckoned as equal to that contained in the excrementitious matters, the carbon of the above articles was con- sidered as x >eing converted into carbonic acid. obvious. They appear naturally both in man and animals at certain seasons of the year, and we say in such cases that we are freezing, or experience the sensation of cold. It is plain, that if we were to clothe a man in a metallic dress, and tie up his hands and feet, the loss of heat, for the same consump- tion of oxygen, would be far greater than if we were to wrap him up in fur and woollen cloth. Nay, in the latter case, we should see him begin to perspire, and warm water would exude, in drops, through the finest pores of his skin. If to these considerations we add, that de- cisive experiments are on record, in which animals were made to respire in an unna- tural position, as for example, lying on the back, with the limbs tied so as to preclude motion, and that the temperature of their bodies was found to sink in a degree appre- ciable by the thermometer, we can hardly be at a loss what value we ought to attach to the conclusions drawn from such experi- ments as those above described. These experiments and the conclusions deduced from them, in short, are incapable of furnishing the smallest support to the opinion that there exists, in the animal body, any other unknown source of heat, besides the mutual chemical action between the ele- ments of the food and the oxygen of the air. The existence of the latter cannot be doubted or denied, and it is amply sufficient to ex- plain all the phenomena. VII. If we designate the production ol force, the phenomena of motion in the ani- mal body as nervous life, and the resistance, the condition of static equilibrium, as vege- tative life ; it is obvious that in all classes of animals the latter, namely, vegetative life, prevails over the former, nervous life, in the earlier stages of existence. The passage or change of matter from a state of motion to a state of rest appears in an increase of the mass, and in the supply of waste ; while the motion itself, or the production offeree, appears in the shape of waste of matter. In a young animal, the waste is less than the increase ; and the female retains, up to a certain age, this peculiar condition of a more intense vegetative life. This condition does not cease in the female as in the male, with the complete development of all the organs of the body. The female in the lower animals, is, at certain seasons', capable of reproduction of the species. The vegetative life in her or- ganism is rendered more intense by certain external conditions, such as temperature, food,&c. ; the organism produces more than is wasted, and the result is the capacity of reproduction. In the human species, the female organism is independent of those external causes which increase the intensity of vegetative life. When the organism is fully developed, it is at all times capable of reproduction of the species ; and infinite wisdom has given FIBRINE AND ALBUMEN. 21 .o the femaie body the power, up to a certain age, of producing all parts of its organization in greater quantity than is required to sup- ply the daily waste. This excess of production can be shown to contain all the elements of a new organism, it is constan-tly accumulating, and is periodi- cally expelled from the body, until it is ex- pended in reproduction. This periodical discharge ceases when the ovum has been impregnated, and from this time every drop of the superabundant blood goes to produce an organism like that of the mother. Exercise and labour cause a diminution in the quantity of the menstrual discharge; and when it is suppressed in consequence of disease, the vegetative life is manifested in a morbid production of fat. When the equilibrium between the vegetative and ner- vous life is disturbed in the male, when, as in eunuchs, the intensity of the latter is di- minished, the predominance of the former is shown in the same form, in an increased deposit of fat. VIII. If we hold,, that increase of mass in the animal body, the development of its or- gans, and the supply of waste, that ail this is dependent on the blood, that is, on the ingredients of the blood, then only those substances can properly be called nutritious, or considered as food which are capable of conversion into blood. To determine, there- fore, what substances are capable of afford- ing nourishment, it is only necessary to as- certain the composition of the food, and to compare it with that of the ingredients of the blood. Two substances require especial conside- ration as the chief ingredients of the blood ; one of these separates immediately from the blood when withdrawn from the circulation. It is well known that in this case blood coagulates, and separates into a yellowish liquid, the serum of the blood, and a gela- tinous mass, which adheres to a rod or stick in soft, elastic fibres, when coagulating blood is briskly stirred. This is ihejibrine of the blood, which is identical in all its properties with muscular fibre, when the latter is pu- rified from all foreign matters. The second principal ingredient of the blood is contained in the serum, and gives to this liquid all the properties of the white of eggs, with which it is identical. When heated, it coagulates into a white elastic mass, and the coagulating substance is called albumen. Fibrine and albumen, the chief ingredients of blood, contain, in all, seven chemical elements, among which nitrogen, phos- phrus, and sulphur are found. They con- tain also the earth of bones. The serum retains in solution sea salt and other salts of potash and soda, in which the acids are carbonic, phosphoric, and sulphuric acids. The globules of the blood contain fibrine and albumen, along with a red colouring matter, in which iron is a constant element. Be- side these, the blood contains certain fatty bodies in small quantity, which differ from ordinary fats in several of their properties. Chemical analysis has led to the remark- able result, that fibrine and albumen contain the same organic elements united in the same proportion, so that two analyses, the one of fibrine and the other of albumen, do not differ more than two analyses of fibrine or two of albumen respectively do, in the composition of 100 parts. In these two ingredients of blood the par- ticles are arranged in a different order, as is shown by the difference of their external properties ; but in chemical composition, in the ultimate proportion of the organic ele- ments, they are identical. This conclusion has lately been beautifully confirmed by a distinguished physiologist (Denis,) who has succeeded in converting fibrine into albumen, that is, in giving it the solubility, and coagulability by heat, which characterize the white of egg. Fibrine and albumen, besides having the same composition, agree also in this, that both dissolve in concentrated muriatic acid, yielding a solution of an intense purple colour. This solution, whether made with fibrine or albumen, has the very same re- actions with all substances yet tried. Both albumen and fibrine, in the process of nutrition, are capable of being converted into muscular fibre, and muscular fibre is capable of being reconverted into blood. These facts have long been established by physiologists, and chemistry has merely proved that these metamorphoses can be accomplished under the influence of a cer- tain force, without the aid of a third sub- stance, or of its elements, and without the addition of any foreign element, or the sepa- ration of any element previously present in these substances. If we now compare the composition of all organized parts with that of fibrine and albu- men, the following relations present them- selves : All parts of the animal body which have a decided shape, which forms parts of or- gans, contain nitrogen. No part of an organ which possesses motion and life is destitute of nitrogen ; all of them contain likewise carbon and the elements of water, the latter, however, in no case in the proportion to form water. The chief ingredients of the blood contain nearly 17 per cent, of nitrogen, and no part of an organ contains less than 17 per cent, of nitrogen. (7) The most convincing experiments and observations have proved that the animal body is absolutely incapable of producing an elementary body, such as carbon or ni- trogen, out of substances which do not con- tain it ; and it obviously follows, that all kinds of food fit for the production either of blood, or of cellular tissue, membranes, skin, hair, muscular fibre, &c., must contain a certain amount of nitrogen, because that element is essential to the composition of 22 ANIMAL CHEMISTRY. the above named organs; because the or- gans cannot create it from the other elements presented to them; and, finally, because no nitrogen is absorbed from the atmosphere in the vital process. The substance of the brain and nerves contains a large quantity of albumen,, and, in addition to this, two peculiar fatty acids, distinguished from other fats by containing phosphorus (phosphoric acid ?) One of these contains nitrogen (Fremy.) Finally, water and common fat are those ingredients of the body which are destitute of nitrogen. Both are amorphus or unor- ganized, and only so far take part in the vital process as that their presence is re- quired for the due performance of the vital functions. The inorganic constituents of the body are, iron, lime, magnesia, common salt, and the alkalies. IX. The nutritive process in the carni- vora is seen in its simplest form. This class of animals lives on the blood and flesh of the graminivora; but this blood and flesh is, in all its properties, identical with their own. Neither chemical nor physiological differences can be discovered. The nutriment of carnivorous animals is derived originally from blood ; in their sto- mach it becomes dissolved, and capable of reaching all other parts of the body ; in its passage it is again converted into blood, and from this blood are reproduced all those parts of their organization which have undergone change or metamorphosis. With the exception of hoofs, hair, fea- thers, and the earth of bones, every part of the food of carnivorous animals is capable of assimilation. In a chemical sense, therefore, it may be said that a carnivorous animal, in support- ing the vital process, consumes itself. That which serves for its nutrition is identical with those parts of its organization which are to be renewed. The process of nutrition in graminivorous animals appear at first sight altogether dif- ferent. Their digestive organs artre less sim- ple, and their food consists of vegetables, the great mass of which contains but little nitrogen. From what substances, it may be asked, is the blood formed, by means of which their organs are developed 1 This question may be answered with certainty. Chemical researches have shown, that all such parts of vegetables as can afford nutri- ment to animals contain certain constituents which are rich in nitrogen ; and the most ordinary experience pr,oves that animals re- quire for their support and nutrition less of these parts of plants in proportion as they abound in the nitrogenized constituents. Animals cannot be fed on matters destitute of these nitrogenized constituents. These important products of vegetation are especially abundant in the seeds ol the different kinds of grain, and of pease, beans, and lentils; ia the roots and the juices of what are commonly called vegetables. They exist, howeve.r, in all plants, without excep- tion, and in every part of plants in larger or smaller quantity. These nitrogenizea forms of nutriment in the vegetable kingdom may be reduced to three substances, which are easily distin- guished by their external characters. Two of them are soluble in water, the third is insoluble. When the newly expressed juices of vegetables are allowed to stand, a separation takes place in a few minutes. A gelatinous precipitate, commonly of a green tinge, is deposited, and this, when acted on by liquids which remove the colouring matter, leaves a grayish white subtance, well known to druggists as the deposit from vegetable juices. This is one of the nitrogenized compounds which serves for the nutrition of animals, and has been named vegetable fibrine. The juice of grapes is especially rich in this constituent, but it is most abundant in the seeds of wheat, and of the cerealia. It may be obtained from wheat flour by a mechani- cal operation, and in a state of tolerable purity ; it is then called gluten, but the glutin- ous property belongs, not to vegetable fibrine, but to a foreign substance, present in small quantity, which is not found in the other cerealia. The method by which it is obtained suffi- ciently proves that it is insoluble in water; although we cannot doubt that it was origi- nally dissolved in the vegetable juice, from which it afterwards separated, exactly as fibrine does from blood. The second nitrogenized compound re- mains dissolved in the juice after the sepa- ration of the fibrine. It does not separate from the juice at. the ordinary temperature, but is instantly coagulated when the liquid containing it is heated to the boiling point. When the clarified juice of nutritious vegetables, such as cauliflower, asparagus, mangel wurzel, or turnips, is made to boil, a coagulum is formed, which it is absolutely impossible to distinguish from the substance which separates as coagulum, when the serum of blood or the white of an egg, diluted with water, are heated to the boiling point. This is vegetable albumen. It is found in the greatest abundance in certain seeds, in nuts, almonds, and others, in which the starch of the grammese is re- placed by oil. The third nitrogenized constituent of the vegetable food of animals is vegetable caserne. It is chiefly found in the seeds of pease, beans, lentils,, and similar leguminous seeds. Like vegetable albumen, it is soluble in water, but differs from it in this, that its solution is not coagulated by heat. When the solution is heated or evaporated, a skin forms on its surface, and the addition of an acid causes a coagulum, just as in animal milk. These three nitrogenized compounds, ve- getable fibrine, albumen, and caserne, are USES OF THE STARCH, SUGAR, &c. the true nilrogenized constituents of the food of graminivorous animals j all other nitrogemzed compounds, occurring in plants, are either rejected by animals, as in the case of the characteristic principles of poisonous and medicinal plants, or else they occur in the food in such very small proportion, that they cannot possibly contribute to the in- crease of mass in the animal body. The chemical analysis of these three sub- stances has led to the very interesting result that they contain the same organic elements, united in the same proportion by weight; and, what is still more remarkable, that they are identical in composition with the chief constituents of blood, animal fibrine, and albumen. They all three dissolve in con- centrated muriatic acid with the same deep purple colour, and even in their physical characters, animal fibrine and albumen are in no respect different from vegetable fibrine and albumen. It is especially to be noticed, that by the phrase, identity of composition we do not here imply mere similarity, but that even in regard to the presence and relative amount of sulphur, phosphorus, and phosphate of lime, no difference can be observed. (8) How beautifully and admirably simple, with the aid of these discoveries, appears the process of nutrition in animals, the forma- tion of their organs, in which vitality chiefly resides ! Those vegetable principles, which in animals are used to form blood, contain the chief constituents of blood, fibrine and albumen, ready formed, as far as regards their composition. All plants, besides, con- tain a certain quantity of iron, which re- appears in the colouring matter of the blood. Vegetable fibrine and animal fibrine, veget- able albumen and animal albumen, hardly differ even in form ; if these principles be wanting in the food, the nutrition of the animal is arrested ; and when they are pre- sent, the graminivorous animal obtains in its food the very same principles on the pre- sence of which the nutrition of the car- nivora entirely depends. Vegetables produce in their organism the blood of all animals, for the carnivora, in consuming the blood and flesh of the grami- nivora, consume, strictly speaking, only the vegetable principles which have served for the nutrition of the latter. Vegetable fibrine and albumen take the same form in the stomach of the graminivorous animal as animal fibrine and albumea do in that of the carnivorous animal. From what has been said, it follows that the development of the animal organism and its growth are dependent on the reception of certain principles identical with the chief constituents of blood. In this sense we may say that the animal organism gives to blood only its form ; that it is incapable of creating blood out of other substances which do not already contain the chief constituents of that fluid. We t, indeed, maintain tha. f the anima 1 organism has no power to form other com- pounds, for we know that it is capable of producing an extensive series of compounds, differing in composition from the chief con- stituents of blood \ but these last, which form the starting point of the series, it cannot produce. The animal organism is a higher kind of vegetable, the development of which begins with those substances, with the production of which the life of an ordinary vegetable ends. As soon as the latter has borne seed, it dies, or a period of its life comes to a ter- mination. In that endless series of compounds, which begins with carbonic acid, ammonia, and water, the sources of the nutrition of veget- ables, and includes the most complex consti- tuents of the animal brain, there is no blank, no interruption. The first substance capable of affording nutriment to animals is the last product of the creative energy of vegetables. The substance of cellular tissue and of membranes, of the brain and nerves, these the vegetable cannot produce. The seemingly miraculous in the produc- tive agency of vegetables disappears in a great degree, when we reflect that the pro- duction of the constituents of blood cannot appear more surprising than the occurrence of the fat of beef and mutton in cocoa beans, of human fat in olive oil, of the principal ingredient of butter in palm oil, and of horse fat and train oil in certain only seeds. X. While the preceding considerations leave little or no doubt as to the way in which the increase of mass in an animal, that is, its growth, is carried on, there is yet to be resolved a most important question, "namely, that of the function performed in the animal system by substances containing no nitrogen, such as sugar, starch, gum, pectine, &,c. The most extensive class of animals, the graminivora, cannot live without these sub- stances ; their food must contain a certain amount of one or more of them, and if these compounds are not supplied, death quickly ensues. This important inquiry extends also to the constituents of the food of carnivorous ani- mals in the earliest periods of life ; for this food also contains substances which are not necessary for their support in the adult state. The nutrition of the young qf carnivora is obviously accomplished by means similar to those by which the graminivora are nou- rished ; their development is dependant on the supply of a fluid, which the body of the mother secretes in the shape of milk. Milk contains only one nitrogenized con- stituent, known under the name of caseine ; besides this, its chief ingredients are butler, (fat), and sugar of milk. The blood of the young animal, its mus- cular fibre, cellular tissue, nervous matter, and bones, must have derived their origin from the nitrogenized constituent of milk, the caseine ; for butter and sugar of milk contain no nitrogen. 24 ANIMAL CHEMISTRY. Now, the analysis of caseine has led to the i stricter, a goat, a rabbit, or a bird, we find result, which, after the details given in tne last section, can hardly excite surprise, that this substance also is identical in composi- tion with the chief constituents of blood, fibrine and albumen. Nay, more, a com- parison of its properties with those of veget- able caseine has shown that these two sub- stances are identical in all their properties ; insomuch that certain plants, such as peas, beans, and lentils, are capable of producing the same substance which is formed from the blood of the mother, and employed in yielding the blood of the young animal. (9) The young animal, therefore, receives, in the form of caseine, which is distinguished from fibrine and albumen by its great solu- bility, and by not coagulating when heated, the chief constituent of the mother's blood. To convert caseine into blood no foreign substance is required, and in the conversion of the mother's blood into caseine, no ele- ments of the constituents of the blood have been separated. When chemically ex- amined, caseine is found to contain a much larger proportion of the earth of bones than blood does, and that in a very soluble form, capable of reaching every part of the body. Thus, even in the earliest period of its life, the development cf the organs, in which vi- tality resides, is, in the carnivorous animal, dependant on the supply of a substance,' identical in organic composition with the chief constituents of its blood. What, then, is the use of the butter and the sugar of milk? How does it happen that these substances are indispensable to life? Butter and sugar of milk contain no fixed bases, no soda or potash. Sugar of milk has a composition closely allied to that of the other kinds of sugar, of starch, and of gum; all of them contain carbon and the elements of water, the latter precisely in the propor- tion to form water. There is added, therefore, by means of these compounds, to the nitrogenized con- stituents of food, a certain amount of carbon, or, as in the case of butter, of carbon and hydrogen ; that is, an excess of elements, which cannot possibly be employed in the production of blood, because the nitrogenized substances contained in the food already contain exactly the amount of carbon which is required for the production of fibrine and albumen. The following considerations will show that hardly a doubt can be entertained, that this excess of carbon alone, or of carbon and hydrogen, is expended in the production of animal heat, and serves to protect the or- ganism from the action of the atmospheric oxygen. XI. In order to obtain a clearer insight into the nature of the nutritive process in both the great classes of animals, let us first consider the changes which the food of the carnivora undergoes in their organism. If we give to an adult serpent, or boa con- tnat the hair, hoofs, horns, feathers, or bones of these animals, are expelled from the body apparently unchanged. They have retained their natural form and aspect, but have be- come brittle, because of all their component parts they have lost only that one which was capable of solution, namely, the gela- tine. Faeces, properly so called, do not occur in serpents any more than in carnivo- rous birds. We find, moreover, that when the serpent has regained its original weight, every other part of its prey, the flesh, the blood, the brain, and nerves, in short, every thing has disappeared. The only excrement, strictly speaking, is a substance expelled by the urinary pas- sage. When dry, it is pure white, like chalk; it contains much nitrogen, and a small quantity of carbonate and phosphate of lime mixed with the mass. This excrement is urate of ammonia, a chemical compound, in which the nitrogen bears to the carbon the same proportion as in bicarbonate of ammonia. For every equi- valent of nitrogen it contains two equiva- lents of carbon. But muscular fibre, blood membranes, and skin, contain four times as much carbon for the same amount of nitrogen, or eight equivalents to one ; and if we add to this the carbon of the fat and nervous substance, it is obvious that the serpent has consumed for every equivalent of nitrogen, much more than eight equivalents of carbon. If now we assume that the urate of am- monia contains all the nitrogen of the animal consumed, then at least six equivalents of carbon, which were in combination with this nitrogen, must have been given out in a dif- ferent form from the two equivalents which are found in the urate of ammonia. Now we know, with perfect certainty, that this carbon has been given out through the skin and lungs, which could only take place in the form of an oxidized product. The excrements of a buzzard which had been fed with beef, when taken out of the rectum, consisted, according to L. Gmelin and Tiedemann, of urate of ammonia. In like manner, the faeces in lions and tigers are scanty and dry, consisting chiefly of bone earth, with mere traces of compounds containing carbon ; but their urine contains, not urate of ammonia, but urea, a compound in which carbon and nitrogen are to each othe-r in the same ratio as in neutral carbon- ate of ammonia. Assuming that their food (flesh, &c.) contains carbon and nitrogen in the ratio of eight equivalents to one, we find these ele- ments in their urine in the ratio of one equi- valent to one ; a smaller proportion of car- bon, therefore, than in serpents, in which respiration is so much less active. The whole of the carbon and hydrogen which the food of these animals contained, beyond the amount which we find in their FOOD OF CARNIVORA. excrements, has disappeared, in the process of respiration, as carbonic acid and water. Had the animal food been burned in a furnace, the change produced in it would only have differed in the form of combina- tion assumed by the nitrogen from that which it underwent in- the body of the ani- mal. The nitrogen would have appeared, with part of the carbon and hydrogen, as carbonate of ammonia, while the rest of the carbon and hydrogen would have formed carbonic acid and water. The incombusti- ble parts would have taken the form of ashes, and any part of the carbon uncon- sumed from a deficiency of oxygen would have appeared as soot, or lamp-black. Now the solid exciements are nothing else than the incombustible, or imperfectly burned, parts of the food. In the preceding pages it has been as- sumed that the elements of the food are con- verted by the oxygen absorbed in the lungs into oxidized products; the carbon into car- bonic acid, the hydrogen into water, and the nitrogen into a compound containing the same elements as carbonate of ammonia. This is only true in appearance ; the body, no doubt, after a certain time, acquires its original weight. The amount of carbon, and of the other elements, is not found to be increased exactly as much carbon, hydro- gen, and nitrogen has been given out as was supplied in the food ; but nothing is more certain than that the carbon, hydrogen, and nitrogen given out, although equal in amount to what is supplied in that form, do not directly proceed from the food. It would be utterly irrationable to suppose that the necessity of taking food, or the satisfying the appetite, had no other object than the production of urea, uric acid, car- bonic acid, and other excrementitious mat- ters of substances which the system expels, and consequently applies to no useful pur- pose in the economy. In the adult animal, the food serves to re- store the waste of matter; certain parts of its organs have lost the state of vitality, have been expelled from the substance of the organs, and have been metamorphosed into new combinations, which are amor- phous and unorganized. The food of the carnivora is at once con- verted into blood ; out of the newly formed blood those parts of organs which nave un- dergone metamorphoses are reproduced. The carbon and nitrogen of the food thus become constituent parts of organs. Exactly as much carbon and nitrogen is supplied to the organs by the blood, that is, ultimately, by the food, as they have lost by the transformations attending the exercise of their functions. What then, it may be asked, becomes of the new compounds produced by the trans- formations of the organs, of the muscles, of the membranes and cellular tissue of the nerves and brain? These new compounds cannot, owing to 4 their solubility, remain in the situation where they are formed, for a well known force, namely the circulation of the blood, opposes itself to this. By the expansion of the heart, an organ in which two systems of tubes meet, which are ramified in a most minute network of vessels through all parts of the body, there is produced a vacuum, the immediate effect of which is, that all fluids which can pene- trate into these vessels are urged with great force towards one side of the heart by the external pressure of the atmosphere. This motion is powerfully assisted by the con- traction of the heart, alternating with its ex- pansion, and caused by a force independent of the atmospheric pressure. In a word, the heart is a forcing pump, which sends arterial blood into all "parts of the body; and also a suction pump, by means of which all fluids of whatever kind, as soon as they enter the absorbent vessels which communicate with the veins, are drawn towards the heart. This suction, arising from the vacuum caused by the ex- pansion of the heart, is a purely mechanical act, which extends, as above stated, to fluids of every kind, to saline solutions, poisons, &c. It is obvious, therefore, that by the forcible entrance of arterial blood into the capillary vessels, the fluids contained in these, in other words, the soluble compounds formed by the transformations of organized parts, must be compelled to move towards the heart. These compounds cannot be employed for the reproduction of those tissues from which they are derived. They pass through the absorbent and lymphatic vessels into the veins, where their accumulation would speedily put a stop to the nutritive process, were it not that this accumulation is pre- vented by two contrivances adapted ex- pressly to this purpose, and which may be compared to filtering machines. The venous blood, before reaching the heart, is made to pass through the liver; the arterial blood, on the other hand, passes through the kidneys ; and these organs sepa- rate from both all substances incapable of contributing to nutrition. Those new compounds which contain the nitrogen of the transformed organs are col- lected in the urinary bladder, and being ut- terly incapable of any further application in the system, are expelled from the body. Those, again, which contain the carbon of the transformed tissues, are collected in the gall bladder in the form of a compound of soda, the bile, which is miscible with water in every proportion, and which, pass- ing into the duodenum, mixes with the chyme. All those parts of the bile which, during the digestive process, do not lose their solubility, return during that process into the circulation in a state of extreme di- vision. The soda of the bile, and those highly carbonized portions which are not precipitated by a weak acid (together making 26 ANIMAL CHEMISTRY. O f the solid contents of the bile,) re tain the capacity of resorption by the ab- sorbents of the small and large intestines nay, this capacity has been directly provec by the administration of enemata containing bile, the whole of the bile disappearing with the injected fluid in the rectum. Thus we know with certainty, that the nitrogenized compounds, produced by the metamorphosis of organized tissues, after being separated from the arterial blood by means of the kidneys, are expelled from the body as utterly incapable of further altera- tion ; while the compounds, rich in carbon, derived from the same source, return into the system of carnivorous animals. The food of the carnivora is identical with the chief constituents of their bodies, and hence the metamorphoses which their or- gans undergo must be the same as those which, under the influence of the vital force, take place in the matters which constitute their food. The flesh and blood consumed as food yield their carbon for the support of the re- spiratory process, while its nitrogen appears as uric acid, ammonia, or urea. But pre- viously to these final changes, the dead flesh and blood become living flesh and blood, and it is, strictly speaking, the carbon of the compounds formed in the metamorphoses of living tissues that serves for the produc- tion of animal heat. The food of the carnivora is converted into blood, which is destined for the repro- duction of organized tissues ; and by means of the circulation a current of oxygen is conveyed to every part of the body. The globules of the blood, which in themselves can be shown to take no share in the nutri- tive process, serve to transport the oxygen, which they give up in their passage through the capillary vessels. Here the current of oxygen meets with the compounds pro- duced by the transformation of the tissues, and combines with their carbon to form car- bonic acid, with their hydrogen to form water. Every portion of these substances which escapes this process of oxidation is sent back into the circulation in the form of the bile, which by degrees completely dis- appears. In the carnivora the bile contains the car- bon of the metamorphosed tissues ; this carbon disappears in the animal body, and the bile likewise disappears in the vital pro- cess. Its carbon and hydrogen are given out through the skin and lungs as carbonic "cid and water ; and hence it is obvious that uie elements of the bile serve for respiration and for the production of animal heat. Svery part of the food of carnivorous ani- mals is capable of forming blood ; their ex- crements, excluding the urine, contain only inorganic substances, such as phosphate of lime ; and the small quantity of organic mat- ter which is found mixed with these is de- rived from excretions, the use of which is j to promote their passage through the intes- ] tines, such as mucus. These excrements contain no bile and no soda ; for water ex- tracts from them no trace of anv substance resembling bile, and yet bile is very soluble in water, "and mixes with it in every pro- portion. Physiologists can entertain no doubt as to the origin of the constituent parts of the urine and of the bile. When, from the de- privation of food, the stomach contracts itself so as to resemble a portion of intes- tine, the gall-bladder, for want of the motion which the full stomach gives to it, cannot pour out the bile it contains ; hence in ani- mals starved to death we find the gall-blad- der distended and full. The secretion of bile and urine goes on during the winter sleep of hybernating animals ; and we know that the urine of dogs, fed for three weeks exclusively on pure sugar, contains as much of the most highly nitrogenized constituent, urea, as in the normal condition. (Marchaud. Erdmaun's Journal fur prak- tische Chemie, XIV. p. 495.) Differences in the quantity of urea se- creted in these and similar experiments are explained by the condi'ion of the animal in regard to the amount of the natural motions permitted. Every motion increases the amount of organized tissue which under- goes metamorphosis. Thus after a walk, the secretion of urine in man is invariably increased. The urine of the mammalia, of birds, and of amphibia, contains uric acid or urea ; and the excrements of the mollusca, and of in- sects, as of cantharides and of the butterfly of the silkworm, contain urate of ammonia. This constant occurrence of one or two ni- trogenized compounds in the excretions of animals, while so great a difference exists in their food, clearly proves that these com- pounds proceed from one and the same source. As little doubt can be entertained in re- gard to the function of the bile in the vital process. When we consider, that the ace- tate of potash, given in enema, or simply as a bath for the feet, renders the urine strongly alkaline (Rehberger in Tiedemann's Zeitschrift fur Physiologie, ii. 149,) and that the change which the acetic acid here under- goes cannot be conceived without the addi- ion of oxygen, it is obvious, that the soluble constituents of the bile, prone to change in a high degree as we know them to be, and which, as already stated, cannot be employed 'n the production of blood, must, when re- :urned through the intestines into the circu- ation, in like manner yield to the influence of the oxygen which they meet. The bile s a compound of soda, the elements of which, with the exception of the soda, dis- ippears in the body of a carnivorous animal. In the opinion of many of the most dis- inguished physiologists, the bile is intended iolely to be excreted ; and nothing is more certain, than that a substance containing so very small a proportion of nitrogen can USES OF URINE AND BILE. 27 have no share in the process of nutrition or reproduction of organized tissue. But quantitative physiology must at once and decidedly reject the opinion, that the bile serves no purpose in the economy, and is incapable of further change. No part of any organized structure con- tains soda ; only in the serum of the blood, in the fat of the brain, and in the bile, do we meet with that alkali. When the com- pounds of soda in the blood are converted into muscular fibre, membrane, or cellular tissue, the soda they contain must enter into new combinations. The blood which is transformed into organized tissue gives up its soda to the compounds formed by the metamorphoses of the previously existing tissues. In the bile we find one of those compounds of soda. Were the bile intended merely for excre- tion,, we should find it, more or less altered, and also the soda it contains, in the solid excrements. But, with the exception of common salt, and of sulphate of soda, which occur in all the animal fluids, only mere traces of soda are to be found in the faeces. The soda of the bile, therefore, at all events, must have returned from the in- testinal canal into the organism, and the same must be true of the 'organic matters which were in combination with it. According to the observations of physio- logists, a man secretes daily from 17 to 24 oz. of bile ; a large dog, 36 oz. ; a horse 37 Ibs. l Burdach's Physiologic, v. p. 260.) But the faeces of a man do not on an average weigh more than 5^ oz. ; and those of a horse 28i Ibs., of which 21 Ibs. are water, and 7 Ibs. dry fasces. (Boussingault.) The latter yield to alcohol only ^th part of their weight of soluble matter. If we assume the bile to contain 90 per cent, of water, a horse secretes daily 592 oz. of bile, containing 59*2 oz. of solid matter ; while 1\ Ibs. or 120 oz. of dried excrement yield only 6 oz. of matter soluble in alcohol, which might possibly be bile. But this matter is not bile ; when the alcohol is dissi- pated by evaporation, there remains a soft, unctuous mass, altogether insoluble in water, and which, when incinerated, leaves no al- kaline ashes, no soda. (10.) During the digestive process, therefore, ihe soda of the bile, and, along with it, all the soluble parts of that fluid, are returned into the circulation. This soda re-appears in the newly-formed blood, and, finally, we find it in the urine in the form of phosphate, carbonate, and hippurate of soda. Berzelius found in 1 ,000 parts of fresh human fasces only nine parts of substance similar to bile; 5 ounces, therefore, would contain only 21 grains of dried bile, equivalent to 219 grains of fresh bile. But a man secretes daily from 9,640 to 11,520 grains of fluid bile, that is, from 45 to 56 times as much as can be detected in the matters discharged by the intestinal canal. Whatever opinion we may entertain of the accuracy of the physiological experi- ments, in regard to the quantity of bile se- creted by the different classes of animals j thus much is certain, that even ihe maxi- | mum of supposed secretion, in man and in the horse, does not contain as much carbon as is given out in respiration. With all the fat which is mixed with it, or enters into it3 composition, dried bile does not contain more than 69 per cent, of carbon. Conse- quently, if a horse secretes 57 Ibs. of bile, this quantity will contain only 40 ounces of carbon. But the horse expires daily nearly twice as much in the form of carbonic acid. A precisely similar proportion holds good in man. Along with the matter destined for the formation or reproduction of organs, the cir- culation conveys oxygen to all parts of the body. Now, into whatever combination the oxygen may enter in the blood, it must be held as certain, that such of the constituents of blood as are employed for reproduction, are not materially altered by it. In muscular fibre we find fibrine, with all the properties it had in venous blood ; the albumen in the blood does not combine with oxygen. The oxygen may possibly serve to convert into the gaseous state some unknown constituent of the blood; but those well-known con- stituents, which are employed in reproduc- tion, cannot be destined to support the respi- ratory process ; none of their properties can justify such an opinion. Without attempting in this place to ex- haust the whole question of the share taken by the bile in the vital operations, it follows, as has been observed, from the simple com- parison of those parts of the food of the car- nivora which are capable of assimilation, with the ultimate products into which it is converted, that all the carbon of the food, except that portion which is found in the urine, is given out as carbonic acid. But this carbon was ultimately derived from the substance of the metamorphosed tissues; and this being admitted, the ques- tion of the necessity of substances contain- ing much carbon and no nitrogen in the food of the young of the carnivora, and in that of the graminivora, is resolved in a strikingly simple manner. XII. It cannot be disputed that in an adult carnivorous animal, which neither gains nor loses weight perceptibly from day to day, its nourishment, the waste of organ- ized tissue, and its consumption of oxygen, stand to each other in a well-defined and fixed relation. The carbon of the carbonic acid given off, with that of the urine ; the nitrogen of the urine, and the hydrogen given off as am- monia and water; these elements, taken together, must be exactly equal in weight to the carbon, nitrogen, and hydrogen of the metamorphosed tissues, and since these last are exactly replaced by the food, to the car- bon, nitrogen, and hydrogen of the food. Were this not the case, the weight of the 28 ANIMAL CHEMISTRY. animal could not possibly remain un- changed. But, in the young of the carnivora, the weight does not remain unchanged ; on the contrary, it increases from day to day by an appreciable quantity. This fact presupposes, that the assimila- tive process in the young animal is more energetic, more intense, than the process of transformation in the existing tissues. If both processes were equally active, the weight of the body could not increase ; and were the waste by transformation greater, the weight of the body would decrease. Now, the circulation in the young animal is not weaker, but, on the contrary, more rapid ; the respirations are more frequent ; and, for equal bulks, the consumption of oxygen must be greater rather than smaller in the young than in the adult animal. But, since the metamorphosis of organized parts goes on more slowly, there would ensue a deficiency of those substances, the carbo-n and hydrogen of which are adapted for com- bination with oxygen ; because, in the car- nivora it is the new compounds, produced by the metamorphosis of organized parts, which nature has destined to furnish the ne- cessary resistance to the action of the oxy- gen, and to produce animal heat. What is wanting for these purposes an infinite wis- dom has supplied to the young animal in its natural food. The carbon and hydrogen of butter, and the carbon of the sugar of milk, no part of either of which can yield blood, fibrine, or albumen, are destined for the support of the respiratory process, at an age when a greater resistance is opposed to the metamorphosis of existing organisms; or, in other words, to the production of compounds, which in the adult state are produced in quantity amply sufficient for the purpose of respira- tion. The young animal receives the constitu- ents of its blood in the caseine of the milk. A metamorphosis of existing organs goes on, for bile and urine are secreted ; the matter of the metamorphosed parts is given off in the'form of urine, of carbonic acid, and of water; but the butter and sugar of milk also disappear; they cannot be detected in the faeces. The butter and sugar of milk are given out in the form of carbonic acid and water, and their conversion into oxidized products furnishes the clearest proof that far more oxygen is absorbed than is required to con- vert the carbon and hydrogen of the meta- morphosed tissues into carbonic acid and water. The change and metamorphosis of organ- ized tissues going on in the vital process in the young animal, consequently yield, in a given time, much less carbon and hydrogen in the form adapted for the respiratory pro- cess than corresponds to the oxygen taken up in the lungs. The substance of its organized parts would undergo a more rapid consump- tion, and would necessarily yield to the action of the oxygen, were not the deficiency of carbon and hydrogen supplied from another source. The continued increase of mass, or growth, and the free and unimpeded de- velopement of the organs of the young animal, are dependent on the presence of foreign substances, which, in the nutritive process, have no other function than to pro- tect the newly-formed organs from the action of the oxygen. It is the elements of these substances which unite with the oxygen ; the organs themselves could not do so with- out being consumed ; that is, growth, or increase of mass in the body, the consump- tion of oxygen remaining the same, would be utterly impossible. The preceding considerations leave no doubt as to the purpose for which Nature has added to the food of the young of car- nivorous mammalia substances devoid of nitrogen, which their organism cannot em- ploy for nutrition, strictly so called, that is, for the production of blood; substances which may be entirely dispensed with in their nourishment in the adult state. In the young of carnivorous birds, the want of all motion is an obvious cause of diminished waste in the organized parts ; hence, milk is not provided for them. The nutritive process in the carnivora thus presents itself in two distinct forms; one of which we again meet with in the graminivora. XIII. In the class of graminivorous ani- mals, we observe, that during their whole life, their existence depends on the supply of substances having a composition identical with that of sugar of milk, or closely re- sembling it. Every thing that they consume as food contains a certain quantity of starch, or gum, or sugar, mixed with other matters. The most abundant and widely-extended of the substances of this class is amylon or starch ; it occurs in roots, seeds, and stalks, and even in wood, deposited in the form of roundish or oval globules, which differ from each other in size alone, being identical in chemical composition. (11.) In the same plant, in the pea, for example, we find starch, the globules of which differ in size. Those in the expressed juice of the stalks have a diameter of from -g-fr^j- to y-^-g- of an inch, while those in the seeds are three or four times larger. The globules in arrow- root and in potato starch are distinguished by their large size ; those of rice and of wheat are remarkably small. It is well known that starch may be con- verted into sugar by very different means. This change occurs in the process of germi- nation, as in malting, and it is easily accom- plished by the action of acids. The meta- morphosis of starch into sugar depends simply, as is proved by analysis, on the ad- dition of the elements of water. (12.) All the carbon of the starch is found in the sugar; none of its elements have been NUTRITION OP THE GRAMINIVORA. 29 separated, and, except the elements of water, no foreign element has been added to it in this transformation. In many, especially in pulpy fruits, which when unripe are sour and rough to the taste, out when ripe are sweet, as, for example, in apples and pears, the sugar is produced from the starch which the unripe fruit con- tains. If we rub unripe apples or pears on a grater to a pulp, and wash this with cold water on a fine sieve, the turpid liquid which passes through deposits a very fine flour of starch, of which not even a trace can be detected in the ripe fruit. Many varieties become sweet while yet on the tree ; these are the summer or early apples and pears. Others, again, become sweet only after hav- ing been kept for a certain period after gath- ering. The after-ripening, as this change is called, is a purely chemical process, entirely independent of the vitality of the plant. When vegetation ceases, the fruit is capable of reproducing the species, that is, the kernel, stone, or true seed is fully ripe, but the fleshy covering from this period is subjected to the action of the atmosphere. Like all substances in a state of eremacausis, or decay, it absorbs oxygen, and gives off a certain quantity of carbonic acid gas. In the same way as the starch in putre- fying paste, in which it is in contact with decaying gluten, is converted into sugar, the starch in the above-named fruits, in a state of decay, or eremacausis, is trans- formed into grape sugar. The more starch the unripe fruit contains, the sweeter does it become when ripe. A close connexion thus exists between sugar and starch. By means of a variety of chemical actions, which exert no other influence on the elements of starch than that of changing the direction of their mu- tual attraction, we can convert starch into sugar, but it is always grape sugar. tSugar of milk in many respects resembles starch ; (13) it is, by itself, incapable of the vinous fermentation, but it acquires the pro- perty of resolving itself into alcohol and carbonic acid when it is exposed to heat in contact with a substance in the state of fer- mentation (such as putrefying cheese in milk.) In this case, it is first converted into grape sugar; and it undergoes the same transformation, when it is kept in contact with acids with sulphuric acid, for exam- ple at the ordinary temperature. Gum has the same composition in 100 parts as cane sugar. (14.) It is distinguished from the different varieties of sugar by its not possessing the property of being resolved into alcohol and carbonic acid by the pro- cess of putrefaction. When placed in con- tact with fermenting substances, it under- goes no appreciable change, whence we may conclude, with some degree of proba- bility, that its elements, in the peculiar ar- rangement according to which they are united, are held together with a stronger force than the elements of the different kinds of sugar. There is, however, a certain relation be- tween gum and sugar of milk, since both of them, when treated with nitric acid, yield the same oxidized product, namely mucic acid, which cannot, under the same circum- stances, be formed from any of the other kinds of sugar. In order to show more distinctly the simi- larity of composition in these different sub- stances, which perform so important a part in the nutritive process of the graminivora, let us represent one equivalent of carbon by C (=75-8,) and one equivalent of water by aqua (=112-4,) we shall then have for the composition of these substances the follow- ing expressions : Starch . . . =12 C-f-10 aqua. Cane sugar . =12 C-f-10 aqua-f-1 aqua. Gum . . . =12C-i-10aqua-r-l aqua. Sugar of milk . =12 C+10 aqua+2 aqua. Grape sugar . =12 C-j-10 aqua+4 aqua. For the same number of equivalents of carbon, starch contains 10 equivalents, cane sugar and gum 1 1 equivalents, sugar of milk 12 equivalents, and grape-sugar 14 equiva- lents of water, or the elements .of water. XIV. In these different substances, some one of which, is never wanting in the food of the graminivora, there is added to the nitrogenized constituents of this food, to the vegetable albumen, fibrine, and caseine, from which their blood is formed, strictly speaking, only a certain excess of carbon, which the animal organism cannot possibly employ to produce fibrine or albumen, be- cause the nitrogenized constituents of the food already contain the carbon necessary for the production of blood, and because the blood in the body of the carnivora is formed without the aid of this excess of carbon. The function formed in the vital process of the graminivora by these substances (su- gar, gum, &c.) is indicated in a very clear and convincing manner, when we take into consideration the very small relative amount of the carbon which these animals consume in the nitrogenized constituents of their food, which bears no proportion whatever to the oxygen absorbed through the skin and lungs. A horse, for example, can be kept in a perfectly good condition, if he obtains as food 15 Ibs. of hay and 4 Ibs. of oats daily. If we now calculate the whole amount of nitrogen in these matters, as ascertained by- analysis (1-5 per cent, in the hay, 2'2 per cent in the oats,) (15) in the form of blood, that is, as fibrine and albumen, with the due proportion of water in blood, (80 percent.,) the horse receives daily no more than 4^ oz. of nitrogen, corresponding to about 8 Ibs. of blood. But along with this nitrogen, that is, combined with it in the form of fibrine or albumen, the animal receives only about 14 oz. of carbon. Only about 8 oz. of this can be employed to support respiration, for with the nitrogen expelled in the urine there are C* 30 ANIMAL CHEMISTRY. combined, in the form of urea, 3 oz., and in the form of hippuric acid, 3^ oz. of carbon. Without going further into the calculation it will readily be admitted, that the volume of air inspired and expired by a horse, the quantity of oxygen consumed, and, as a necessary consequence, the amount of car- bonic acid given out by the animal, is much greater than in the respiratory process in man. But an adult man consumes daily about 14 oz. of carbon, and the determination of Boussingault, according to which a horse expires 79 oz. daily, cannot be very far from the truth. In the nitrogen ized constituents of his food, therefore, the horse receives rather less than the fifth part of the carbon which his organism requires for the support of the re- spiratory process ; and we see that the wis- dom of the Creator has added to his food the ths which are wanting, in various forms, as, starch, sugar, &c. with which the animal must be supplied, or his organism will be destroyed by the action of the oxygen. It is obvious, that in the system of the gra- minivora, whose food contains so small a proportion, relatively, of the constituents of blood, the process of metamorphosis in ex- isting tissues, and consequently their resto- ration or reproduction, must go on far less rapidly than in the carnivora. Were this not the case, a vegetation a thousand times more luxuriant than the actual one would not suffice for their nourishment. Sugar, gum, and starch would no longer be neces- sary to support life in these animals, be- cause, in that case, the products of the waste, or metamorphosis of the organized tissues, would contain enough of carbon to support the respiratory process. Man, when confined to animal food, re- quires for his support and nourishment ex- tensive sources of food, even more widely extended than the lion and tiger, because, when he has the opportunity, he kills with- out eating. A nation of hunters, on a limited space, is utterly incapable of increasing its num- bers beyond a certain point, which is soon attained. The carbon necessary for respira- tion must be obtained from the animals, of which only a limited number can live on the space supposed. These animals collect from the plants the constituents of their organs and of their blood, and yield them, in turn, to the savages who live by the chase alone. They, again, receive this food unaccompa- nied by those compounds, destitute of nitro- gen, which, during the life of the animals, served to support the respiratory process. In such men, confined to an animal diet, it is the carbon of the flesh and of the blood which must take the place of starch and sugar. But 151bs. of flesh contain not more car- bon than 4 Ibs. of starch, (16) and while the savage with one animal and an equal weight of starch could maintain life and health for a certain number of days, he would be com- , pelled, if confined to flesh, in order to pro* cure the carbon necessary for respiration, during the same time, to consume five such animals. It is easy to see, from these considerations, how close the connexion is between agricul- ture and the multiplication of the human species. The cultivation of our crops has ultimately no other object than the produc- tion of a maximum of those substances which are adapted for assimilation and re- spiration, in the smallest possible space. Grain and other nutritious vegetables yield us, not only in starch, sugar, and gum, the carbon which protects our organs from the action of oxygen, and produces in the or- ganism the heat which is essential to life, but also in the form of vegetable fibrine, al- bumen, and caseine, our blood, from which the other parts of our body are developed. Man, when confined to animal food, re- spires, like the carnivora, at the expense of the matters produced by the metamorphosis of organized tissues; and, just as the lion, tiger, hyaena, in the cages of a menagerie, are compelled to accelerate the waste of the organized tissues by incessant motion, in or- der to furnish the matter necessary for re- spiration, so the savage, for the very same object, is forced to make the most laborious exertions and go through a vast amount of muscular exercise. He is compelled to con- sume force merely in order to supply mat- ter for respiration. Cultivation is the economy offeree. Sci- ence teaches us the simplest means of ob- taining the greatest effect with the smallest expenditure of power, and with given means to produce a maximum of force. The unprofitable exertion, of power, the waste of force in agriculture, in other branches of in- dustry, in science, or in social economy, is characteristic of the savage state, or of the want of cultivation. XV. A comparison of the urine of the carnivora with that of the graminivora shows very clearly, that the process of meta- morphosis in the tissues is different, both in form and in rapidity, in the two classes of animals. The urine of carnivorous animals is acid, and contains alkaline bases united with uric, phosphoric, and sulphuric acids. We know perfectly the source of the two latter acids. All the tissues, with the exception of cellular tissue and membrane, contain phosphoric acid and sulphur, which latter element is converted into sulphuric acid by the oxygen of the arterial blood. In the various fluids of the body there are only traces of phos- phates or sulphates, except in the urine, where both are found in abundance. It is plain that they are derived from the meta- morphosed tissues; they enter into the ve- nous blood in the form of soluble salts, and are separated from it in its passage through the kidneys. The urine of the graminivora is alkaline; it contains alkaline carbonates in abundance, ORIGIN OF FAT IN ANIMALS. 31 and so small a portion of alkaline phos- phates as to have been overlooked by most observers. The deficiency or absence of alkaline phosphates in the urine of the graminivora, obviously indicates the slowness with which the tissues in this class of animals are meta- morphosed j for if we assume that a horse consumes a quantity of vegetable fibrine and albumen corresponding to the amount of nitrogen in his daily food (about 4 oz.,) and that the quantity of tissue metamorphosed is equal to that newly formed, then the quantity of phosphoric acid which on these suppositions would exist in the urine is not so small as not to be easily detected by analy- sis in the daily secretion of urine (3 Ibs. according to Boussingault ;) for it would amount to 0.8 per cent. But, as above staled, most observers have been unable to detect phosphoric acid in the urine of the horse. Hence it is obvious that the phosphoric acid which in consequence of the metamor- phosis of tissues is produced in the form of soluble alkaline phosphates, must re-enter the circulation in this class of animals. It is there employed in forming brain and ner- vous matter, to which it is essential, and also, no doubt, in contributing to the supply of the earthy part of the bones. It is pro- bable, however, that the greater part of the earth of bones is obtained by the direct as- similation of phosphate of lime, while the soluble phosphates are better adapted for the production of nervous matter. In the graminivora, therefore, whose food contains so small a proportion of phos- phorus or of phosphates, the organism col- lects all the soluble phosphates produced by the metamorphosis of tissues, and employs them for the developement of the bones and of the phosphorised constituents of the brain ; the organs of excretion do not sepa- rate these salts from the blood. The phos- phoric acid, which, by the change of matter, is separated in the uncombined state, is not expelled from the body as phosphate of soda ; but we find it in the solid excrements in the form of insoluble earthy phosphates. XVI. If we now compare the capacity for increase of mass, the assimilative power in the graminivora and carnivora, the com- monest observations indicate a very marked difference. A spider, which sucks with extreme vo- racity the blood of the first fly, is not dis- turbed or excited by a second or third. A cat will eat the first, and perhaps the second mouse presented to her, but even if she kills a third, she does not devour it. Exactly similar observations have been made in re- gard to lions and tigers, which only devour their prey when urged by hunger. Carni- vorous animals, indeed, require less food for their mere support, because their skin is destitute of perspiratory pores, and because they consequently lose, for equal bulks, much less heat than graminivorous ani- ! mals, which are compelled to restore the lost heat by means of food adapted for respiration. How different is the energy and intensity of vegetative life in the graminivora. A cow, or a sheep, in the meadow, eats, almost without interruption, as long as the sun is above the horizon. Their system possesses the power of converting into organized tis- sues all the food they devour beyond the quantity required for merely supplying the waste of their bodies. All the excess of blood produced is con- verted into cellular and muscular tissue; the graminivorous animal becomes fleshy and plump, while the flesh of the carnivorous animal is always tough and sinewy. If we consider the case of a stag, a roe- deer, or a hare, animals which consume the same food as cattle and sheep, it is evident that, when well supplied with food, their growth in size, their fattening, must depend on the quantity of vegetable albumen, fibrine, or caseine, which they consume. With free and unimpeded motion and exercise, enough of oxygen is absorbed to consume the carbon of the gum, sugar, starch, and of all similar soluble constituents of their food. But all this is very differently arranged in our domestic animals, when with an abun- dant supply of food, we check the processes of cooling and exhalation, as we do when we feed them in stables, where free motion is impossible. The stall-fed animal eats, and reposes merely for digestion. It devours in the shape of nitrogenized compounds far more food than is required for reproduction, or the supply of waste alone ; and at the same time it eats far more of substances devoid of nitrogen than is necessary merely to sup- port respiration and to keep up animal heat. Want of exercise and diminished cooling are equivalent to a deficient supply of oxy- gen ; for when these circumstances occur, the animal absorbs much less oxygen than is required to convert into carbonic acid the carbon of the substances destined for respiration. Only a small part of the ex- cess of carbon thus occasioned is ex- pelled from the body in the horse and ox, in the form of hippuric acid j and all the re- mainder is employed in the production of a substance which, in the normal state, only occurs in small quantity as a constituent of the nerves and brain. This substance is fat. In the normal condition, as to exercise and labour, the urine of the horse and ox contains benzoic acid (with 14 equivalents of carbon ;) but as soon as the animal is kept quiet in the stable, the urine con- tains hippuric acid, (with 18 equivalents of carbon.) The flesh of wild animals is devoid of fat; while that of stall-fed animals is covered with that substance. When the fattened animal is allowed to move more 32 ANIMAL CHEMISTRY. freely in the air, or compelled to draw heavy Durdens, the fat again disappears. It is evident, therefore, that the formation of fat in the animal body is the result of a want of due proportion between the food taken into the stomach and the oxygen ab- sorbed by the lungs and the skin. A pig, when fed with highly nitrogenized food, becomes full of flesh ; when fed with potatoes (starch) it acquires little flesh, but a thick layer of fat. The milk of a cow, when stall-fed, is very rich in butter, but in the meadow is found to contain more ca- seine, and in the same proportion less butter and sugar of milk. In the human female, beer, and farinaceous diet increase the pro- portion of butter in the milk; an animal diet yields less milk, but it is richer in caseine. If we reflect, that in the entire class of carnivora, the food of which contains no substance devoid of nitrogen except fat, the production of fat in the body is utterly in- significant; that even in these animals, as in dogs and cats, it increases as soon as they live on a mixed diet; and that we can in- crease the formation of fat in other domes- tic animals at pleasure, but only by means of food containing no nitrogen ; we can hardly entertain a doubt that such food, in its various forms of starch, sugar, &c., is closely connected with the production of fat. In the natural course of scientific research, we draw conclusions from the food in re- gard to the tissues or substances formed from it; from the nitrogenized constituents of plants we draw certain inferences as to the nitrogenized constituents of the blood ; and it is quite in accordance with this, the natural method, that we should seek to es- tablish the relations of those parts of our food which are devoid of nitrogen and those parts of the body which contain none of that element. It is impossible to over- look the very intimate connexion between them. If we compare the composition of su^ar of milk, of starch, and of the other varieties of sugar, with that of mutton and beef suet and of human fat, we find that in all of them the proportion of carbon to hydrogen is the same, and that they only differ in that of oxygen. According to the analyses of Chevreul, mutton fat, human fat, and hogs' lard, con- tain 29 percent, of carbon to ll.l, 11.4, and 11.7 per cent, of hydrogen respec- tively. (16) Starch contains 44.91 carbon to 6.11 hydrogen. Gum and sugar 42.58 carbon to 6.37 hydrogen. (17) It is obvious that these numbers, repre- senting the relative proportions of carbon and hydrogen in starch, gum, and sugar, are in the same ratio as the carbon and hy- drogen in the different kinds of fat; for 44-91 : 6-11 =79 : 10-99 42-58 : 6-37 = 79 : 11-80 From which it follows, that sugar, starch, and gum, by the mere separation of a part of their oxygen, may pass into fat, or at least into a substance having exactly the composition of fat. If from the formula of starch, C 12 H 10 O 10 , we take 9 equivalents of oxygen, there will remain in 100 parts C 12 - - - 79-4 H 10 - - - 10-8 O ... 9-8 The empirical formula of fat which comes nearest to this is C U H 10 O, which gives in 100 parts C" . . . 78-9 H 10 - - - 11-6 O ... 9-5 According to this formula, an equivalent of starch, in order to be changed into fat would lose 1 equivalent of carbonic acid, CO 2 , and 7 equivalents of oxygen. Now the composition of all saponifiable fatty bodies agrees very closely with one or other of these two formula?. If from 3 equivalents of sugar of milk, tfH^O'^C^H^O 36 , we take away four equivalents of water and 31 of oxygen, there will remain C 36 H 22 O, a formula which ac- curately represents the composition of cho- esterine, the fat of bile. (18.) Whatever views we may entertain re- garding the origin of the fatty constituents of the body, this much at least is undeni- able, that the herbs and roots consumed by A he cow contain no butter; that in hay or :he other fodder of oxen no beef suet exists ; ;hat no hogs' lard can be found in the po- ;ato refuse given to swine; and that the food of geese or fowls contains no goose fat or appn fat. The masses of fat found in the )odies of these animals are formed in their organism; and when the full value of this ^t is recognised, it entitles us to conclude hat a certain quantity of oxygen, in some "orm or other, separates from the constitu- ents of their food; for without such a sepa- ration of oxygen, no fat could possibly be "ormed from any one of these substances. The chemical analysis of the constituents of the food of the graminivora shows in the clearest manner that they contain carbon and oxygen in certain proportions; which, when reduced to equivalents, yield the fol- owing series : n vegetable fibrine, albumen, and caseine, there are contained, for 1 20 eq. carbon, 36 eq . oxygen, n starch 120 100 n cane sugar 120 110 ngum 120 110 n sugar of milk 120 120 n grape sugar 120 140 Now in all fatty bodies there are contained, m an average For - 120 eq. carb. only 10 eq. oxygen. Since the carbon of the fatty constituents f the animal body is derived from the food, FORMATION OP FAT. 33 seeing that there is no other source whence it can be derived, it is obvious, if we sup- pose fat to be formed from albumen, fibrine, or caseine, that, for every 120 equivalents of carbon deposited as fat, 26 equivalents of oxygen must be separated from the elements of these substances ; and further, if we con- ceive fat to be formed from starch, sugar, or sugar of milk, that for the same amount of carbon there must be separated 90, 100, and 110 equivalents of oxygen from these com- pounds respectively. There is, therefore, but one way in which the formation of fat in the animal body is possible, and this is absolutely the same in which its formation in plants takes place ; it is a separation of oxygen from the elements of the food. The carbon which we find deposited in the seeds and fruits of vegetables, in the form of oil and fat, was previously a constituent of the atmosphere, and was absorbed by the plant as carbonic acid. Its conversion into fat was accomplished under the influence of light, by the vital force of the vegetable ; and the greater part of the oxygen of this car- bonic acid was returned to the atmosphere as oxygen gas.* In contradistinction to this phenomenon of vitality in plants, we know that the ani- mal system absorbs oxygen from the atmo- sphere, and that this oxygen is again given out in combination with carbon or hydrogen ; we know, that in the formation of carbonic acid and water, the heat necessary to sus- tain the constant temperature of the body is produced, and that a process of oxidation is the only source of animal heat. Whether fat be formed by the decomposi- tion of fibrine and albumen, the chief con- stituents of blood, or by that of starch, sugar, or gum, this decomposition must be accom- panied by the separation of oxygen from the elements of these compounds. But this oxygen is not given out in the free state, be- cause it meets in the organism with sub- stances possessing the property of entering into combination with it. In fact, it is given out in the same forms as that which is absorbed from the atmosphere by the skin and lungs. It is easy to see, from the above consider- ations, that a very remarkable connexion exists between the formation of fat and the respiratory process. XVIII. The abnormal condition, which onuses the deposit of fat in the animal body, depends, as was formerly stated, on a dis- proportion between the quantity of carbon in the food and that of oxygen, absorbed by the skin and lungs. In the normal condi- tion, the quantity of carbon given put is exactly equal to that which is taken in the food, and the body acquires no increase of weight from the accumulation of substances containing much carbon and no nitrogen. * See Appendix, No. 19, oil the formation of wax and honey by the bee. 5 If we increase the supply of highly car- bonized food, then the normal state can only- he preserved on the condition that, by exer- cise and labor, the waste of the body is in- creased, and the supply of oxygen aug- mented in the same proportion. The production of fat is always a conse- quence of a deficient supply of oxygen, for oxygen is absolutely indispensable for the dissipation of the excess of carbon in the food. This excess of carbon, deposited in the form of fat, is never seen in the Bedouin or in the Arab of the desert, who exhibits with pride to the traveller his lean, muscu- lar, sinewy limbs, altogether free from fat; but in prisons and jails it appears as a puf- finess in the inmates, fed, as they are, on a poor and scanty diet ; it appears in the se- dentary females of oriental countries ; and finally, it is produced under the well known conditions of the fattening of domestic animals. The formation of fat depends on a defi- ciency of oxygen ; but in this process, in the formation of fat itself, there is opened up a new source of oxygen, a new cause of ani- mal heat. The oxygen set free in the formation of fat is given out in combination with carbon or hydrogen ; and whether this carbon and hydrogen proceed from the substance that yields the oxygen, or from other compounds, still there must have been generated by this formation of carbonic acid or water as much heat as if an equal weight of carbon or hydro- gen had been burned in air or in oxygen gas. If we suppose that from 2 equivalents of starch 18 equivalents of oxygen are disen- gaged, and that these 18 equivalents of oxy- gen combine with 9 equivalents of carbon, from the bile, for example, no one can doubt that, in this case, exactly as much heat must be developed, as if these 9 equivalents of carbon had been directly burned. In this form, therefore, the disengagement of heat as a consequence of the formation of fat would be undeniable ; and it could only be considered hypothetical, on the supposition that carbon and oxygen were disengaged from one and the same substance, in the proportions to yield carbonic acid. If, for example, we suppose that from 2 atoms of starch, C M H W O K , the elements of 9 equivalents of carbonic acid are separated, there will remain a compound containing, for 15 equivalents of carbon, 20 of hydrogen and 2 of oxygen ; for C24H20Q 20 = C 9 18 + C 15 H 20 2 . Or, if we assume that oxygen is separated from starch in the form both of carbonic acid and water, then, after subtracting the elements of 6 equivalents of water and 6 of carbonic acid, there would remain the com- pound C^H^O 2 ; for C ai H 2o O 2o = c*O u + H 6 O 6 + C 19 H 14 O 2 . Assuming, then, the separation of oxygen in either of these forms, it remains to be de- cided whether the carbonic acid and water 34 ANIMAL CHEMISTRY. given off were contained, as such, in the starch, or not. If they were ready formed in the starch the separation might occur without the dis- engagement of heat ; but if the carbon and hydrogen were present in any other form in the starch, (or in the compound from which the fat was produced,) it is obvious that a change in the arrangement of the atoms must have occurred, in consequence of which the atoms of the carbon and of the hydrogen have united with those of the oxygen, to form carbonic acid and water. Now, so far as chemical researches have gone, our knowledge of the constitution of starch, and of the varieties of sugar* will j ustify no other conclusion than this, that these sub- stances contain no ready formed carbonic acid. We are acquainted with a large number of processes of metamorphosis of a similar kind, in which the elements of carbonic acid and water are separated from certain pre- existing compounds; and we know with certainty that all these processes are accom- panied by a disengagement of heat, exactly as if the carbon and hydrogen combined directly with oxygen. Such a disengagement of carbonic acid, for example, occurs in all processes of fer- mentation or putEefaction, which are, with- out exception, accompanied with a genera- tion of heat. In the fermentation of a saccharine solu- tion, in consequence of a new arrangement of the elements of the sugar, a certain part of its carbon and oxygen unite to form car- bonic acid, which separates as gas; and as another result of this decomposition, we ob- tain a volatile combustible liquid, containing little oxygen, namely, alcohol. If we add to 2 equivalents of sugar the elements of 12 equivalents of water, and subtract from the sum of the atoms 24 equi- valents of oxygen, there remain 6 equiva- lents of alcohol. eq. alcohol. These 24 equivalents of oxygen suffice to oxidize completely a third equivalent of sugar that is, to convert its carbon into carbonic acid and its hydrogen into water, and by this oxidation we recover the 12 equivalents of water supposed to be added in the former part of the process, exactly as if this water had taken no share in it. C ia H i2OM.f QM^ 12CO 2 -f 12HO. . According to the ordinary view, 12 equi- valents of carbonic acid separate from 3 of sugar, yielding 6 of alcohol that is, exactly the same amount of these products as if two- thirds of the sugar had yielded oxygen to the remaining third, so as completely to oxidize its elements. 12CO 2 .* * For an explanation of the formula? and equa- tions employed, see the Introduction to the Ap- pendix. By a comparison of these two methods of representing the same change, it will easily be seen that the division or splitting of a compound like sugar into carbonic acid, on the one hand, and a compound containing a little oxygen on the other, is in its results perfectly equivalent to a separation of oxy- gen from a certain portion of the compound, and the oxidation or combustion of another portion of it at the expense of this oxy- gen. It is well known that the temperature of a fermenting liquid rises ; and if we assume that a hogshead of wort, holding 1,200 litres = 2,400 Ibs., French weight, contains 16 per cent, of sugar, in all 384 Ibs., then, dur- ing the fermentation of this sugar, an amount of heat must be generated equal to that which would be produced by the combus- tion of 51 Ibs. of carbon. This is equal to a quantity of heat by which every pound of the liquid might be heated by 297'9; that is, supposing the decomposition of the sugar to occur in a period of time too short to be measured. This is well known not to be the case; the fermentation lasts five or six days, and each pound of liquid receives the 297'9 degrees of heat during a period of 120 hours. In each hour there is, therefore, set free an amount of heat capable of raising the tem- perature of each pound of liquid 1 - 4 degree ; a rise of temperature which is very power- fully counteracted by external cooling and by the vaporization of alcohol and water. The formation of fat, like other analogous phenomena in which oxygen is separated in the form of carbonic acid, is consequently accompanied by a disengagement of heat. This change supplies to the animal body a certain proportion of the oxygen indispens- able to the vital processes; and this espe- cially in those cases in which the oxygen absorbed by the skin and lungs is not suf- ficient to convert into carbonic acid the whole of the carbon adapted for this com- bination. This excess of carbon, as it cannot be employed to form a part of any organ, is deposited in the cellular tissue in the form of tallow or oil. At every period of animal life, when there occurs a disproportion between the carbon of the food and the inspired oxygen, the tatter being deficient, fat must be formed. Oxygen separates from existing compounds, and this oxygen is given out as carbonic acid or water. The heat generated in the forma- tion of these two products contributes to keep up the temperature of the body. Every pound of carbon which obtains the oxygen necessary to convert it into carbonic acid from substances which thereby pass into fat, must disengage as much heat as would raise the temperature of 200 Ibs. of water by 70, that is, from 32 to 102. Thus, in the formation of fat, the vital force possesses a means of counteracting a deficiency in the supply of oxygen, and con- FORMATION OP FAT. 35 sequently in that of the heat indispensable for the vital process. Experience teaches us that in poultry, the maximum of fat is obtained by tying the feet, and by a medium temperature. These animals in such circumstances may be compared to a plant possessing in the highest degree the power of converting all food into parts of its own structure. The excess of the constituents of blood forms flesh and other organized tissues, while that of starch, sugar, &c., is converted into fat. When animals are fattened on food destitute of nitrogen, only certain parts of their struc- ture increase in size. Thus, in a goose, fattened in the method above alluded to, the liver becomes three or four times larger than in the same animal, when well fed with free motion, while we cannot say that the or- ganized structure of the liver is thereby in- creased. The liver of a goose fed in the ordinary way is firm and elastic ; that of the imprisoned animal is soft and spongy. The difference consists in a greater or less ex- pansion of its cells which are filled with fat. In some diseases, the starch, sugar, &,c., of the food obviously do not undergo the changes which enable them to assist in respiration, and consequently to be con- verted into fat. Thus, in diabetes mellitus, the starch is only converted into grape sugar, which is expelled from the body without further change. In other diseases, as for example in in- flammation of the liver, we find the blood loaded with fat and oil; and in the composi- tion of the bile there is nothing at all incon- sistent with the supposition that some of its constituents may be transformed into fat. XIX. According to what has been laid down in the preceding pages, the substances of which the food of man is composed may be divided into two classes ; into nitrogenized and non-nitrogenized. The former are ca- pable of conversion into blood; the latter incapable of this transformation. Out of those substances which are adapted to the formation of blood are formed all the organiz-ed tissues. The other class of sub- stances, in the normal state of health, serve to support the process of respiration. The former may be called the plastic elements of nutrition; the latter, elements of respiration. Among the former we reckon Vegetable fibrine. Vegetable albumen. Vegetable caseine. Animal flesh. Animal blood. Among the elements of respiration in our food, are Fat. Pectine. Starch. Bassorine. Gum. Wine. Cane Sugar. Beer. Grape Sugar. Spirits. Sugar of milk. XX. The most recent and exact re- searches have established as a universal fact, to which nothing yet known is op- posed, that the nitrogenized constituents of vegetable food have a composition identical with that of the constituents of the blood. No nitrogenized compound, the composi- tion of which differs from that of fibrine, albumen, and caseine, is capable of sup- porting the vital process in animals. The animal organism unquestionably pos- sesses the power of forming, from the con- stituents of its blood, the substance of its membranes and cellular tissue, of the nerves and brain, of the organic part of cartilages and bones. But the blood must be supplied to it ready formed in every thing but its form that is, in its chemical composition. If this be not done, a period is rapidly put to the formation of blood, and consequently to life. This consideration enables us easily to explain how it happens that the tissues yielding gelatine or chondrine, as, for ex- ample, the gelatine of skin or of bones, are not adapted for the support of the vital pro- cess ; for their composition is different from that of fibrine or albumen. It is obvious that this means nothing more than that those parts of the animal organism which form the blood do not possess the power of effect- ing a transformation in the arrangement of the elements of gelatine, or of those tissues which contain it. The gelatinous tissues, the gelatine of the bones, the membranes, the cells, and the skin, suffer, in the animal body, under the influence of oxygen and moisture, a progressive alteration ; a part of these tissues is separated, and must be restored from the blood ; but this alteration and restoration is obviously confined within very narrow limits. While, in the body of a starving or sick individual, the fat disappears, and the mus- cular tissue takes once more the form of blood, we find that the tendons and mem- branes retain their natural condition ; the limbs of the dead body retain their connex- ions, which depend on the gelatinous tis- sues. On the other hand, we see that the gelatine of bones devoured by a dog entirely disap- pears, while only the bone earth is found in his excrements. The same is true of man, when fed on food rich in gelatine, as, for example, strong soup. The gelatine is not to be found either in the urine or in the faeces, and consequently must have under- gone a change, and must have served some purpose in the animal economy. It is clear, that the gelatine must be expelled from the body in a form different from that in which it was introduced as food. When we consider the transformation ot the albumen of the blood into a part of an organ composed of fibrine, the identity in composition of the two substances renders the change easily conceivable. Indeed we find the change of a dissolved substance into an insoluble organ of vitality, chemically speaking, natural and easily explained, oil 36 ANIMAL CHEMISTRY. account of this very identity of composition. Hence the opinion is not unworthy of a closer investigation, that gelatine, when taken in the dissolved state, is again con- verted, in the body, into cellular tissue, membrane and cartilage ; that it may serve for the reproduction of such parts of these tissues as have been wasted, and for their growth. And when the powers of nutrition in the whole body are affected by a change of the health, then, even should the power of form- ing blood remain the same, the organic force by which the constituents of the blood are transformed into cellular tissue and mem- branes must necessarily be enfeebled by sickness. In the sick man, the intensity of the vital force, its power to produce meta- morphoses, must be diminished as well in the stomach as in all other parts of the body. In this condition, the uniform experience of practical physicians shows that gelatinous matters in a dissolved state exercise a most decided influence on the state of the health. Given in a form adapted for assimilation, they serve to husband the vital force, just as may be done, in the case of the stomach, by due preparation of the food in general. Brittleness in the bones of graminivorous animals is clearly owing to a weakness in those parts of the organism whose function it is to convert the constituents of the blood into cellular tissue and membrane ; and if we can trust to the reports of physicians who have resided in the East, the Turkish women, in their diet of rice, and in the fre- quent use of enemata of strong sotfp, have united the conditions necessary for the formation both of cellular tissue and of fat. PART II. THE METAMORPHOSIS OF TISSUES. 1. THE absolute identity of composition in the chief constituents of blood and the ni- trogenized compounds in vegetable food would, some years ago, have furnished a plausible reason for denying the accuracy of the chemical analysis leading to such a re- sult. At that period, experiment had not as yet demonstrated the existence of numerous compounds, both containing nitrogen and devoid of that element, which with the greatest diversity in external characters, yet possess the very same composition in 100 parts; nay, many of which even contain the same absolute amount of equivalents of each element. Such examples are now very fre- quent, and are known by the names of isomeric and. polymeric compounds. 2. Cyanunc acid, for example, is a nitro- genized compound which crystallizes in beautiful transparent octahedrons, easily so- luble in water and in acids, and very per- manent. Cyamelide is a second body, abso- lutely insoluble in water and acids, white and opaque like porcelain or magnesia. Hydrated cyanic" acid is a third compound, which is a liquid more volatile than pure acetic acid, which blisters the skin, and can- not be brought in contact with water with- out being instantaneously resolved into new products. These three substances not only yield, on analysis, absolutely the same rela- tive weights of the same elements, but they may be converted and reconverted into one another, even in hermetically closed vessels that is, without the aid of any foreign matter. (See Appendix, 21.) Again, among those substances which contain no nitrogen, we have aldehyde, a combustible liquid mis- cible with water, which boils at the tempe- rature of the hand, attracts oxygen from the atmosphere with avidity, and is thereby changed into acetic acid. Tins compound cannot be preserved, even in close vessels j for after some hours or days, its consistence, its volatility, and its power of absorbing oxygen, all are changed. It deposits long, hard, needle-shaped crystals, which at 212 are not volatilized, and the supernatant liquid is no longer aldehyde. It now boils at 140, cannot be mixed with water, and when cooled to a moderate degree crystallizes in a form like ice. Nevertheless, analysis has proved, that these three bodies, so different in their characters, are identical in composi- tion. f21.) 3. A similar group of three occurs in the case of albumen, fibrine, and caseine. They differ in external character, but contain exactly the same proportions of organic ele- ments. When animal albumen, fibrine, and ca- seine are dissolved in a moderately strong solution of caustic potash, and the solution is exposed for some time to a high tempera- ture, these substances are decomposed. The addition of acetic acid to the solution causes, in all three, the separation of a gelatinous translucent precipitate, which has exactly the same characters and composition, from whichever of the three substances above mentioned it has been obtained. Mulder, to whom we owe the discovery of this compound, found, by exact and care- ful analysis, that it contains the same organic elements, and exactly in the same propor- tion, as the animal matters from which it is prepared ; insomuch, that if we deduct from the analysis of albumen, fibrine, and caseine, the ashes they yield when incinerated, as well as the sulphur and phosphorus they contain, and then calculate the remainder for 100 parts, we obtain the same result as DIGESTION COMPARED TO FERMENTATION. 37 in the analysis of the precipitate above de- scribed, prepared by potash, which is free from inorganic matter. (22.) Viewed in this light, the chief constituents of the blood and the caseine of milk may be regarded as compounds of phosphates and other salts, and of sulphur and phosphorus, with a compound of carbon, nitrogen, hy- drogen, and oxygen, in which the relative proportion of these elements is invariable; and this compound may be considered as the commencement and starting point of all other animal tissues, because these are all produced from the blood. These considerations induced Mulder to give to this product of the decomposition of albumen, &,c., by potash, the name of pro- teine (from Trepnvju* , " I take the first rank.") The blood, or the constituents of the blood, are consequently compounds of this proteine with variable proportions of inorganic sub- stances. Mulder further ascertained, that the in- soluble nitrogenized constituent of wheat flour (vegetable fibrine,) when treated with potash, yields the very same product, pro- teine; and it has recently been proved that vegetable albumen and caseine are acted on by potash precisely as animal albumen and caseine are. 4. As far, therefore, as our researches have gone, it may be laid down as a law, founded on experience, that vegetables pro- duce, in their organism, compounds of pro- teine ; and that put of these compounds of proteine the various tissues and parts of the animal body are developed by the vital force, with the aid of the oxygen of the atmosphere and of the elements of water.* Now, although it cannot be demonstrated that proteine exists ready formed in these vegetable and animal products, and although the difference in their properties seems to in- dicate that their elements are not arranged in the same manner, yet the hypothesis of the pre-existence of proteine, as a point of departure in developing and comparing their properties, is exceedingly convenient. At all events it is certain that the elements of these compounds assume the same arrange- ments when acted on by potash at a high temperature. All the organic nitrogenized constituents of the body, how different soever they may be in composition, are derived from proteine. They are formed from it, by the addition or subtraction of the elements of water or of oxygen, and by resolution into two or more compounds. * The experiment of Tiedemann and Gmelin, who found it impossible to sustain the life of geese by means of boiled white of egg, may be easily explained, when we reflect that a graminivorous animal, especially when deprived of free motion, cannot obtain, from the transformation or waste of the tissues alone, enough of carbon for the re- spiratory process. 2 Ibs. of albumen contain only 3^ oz. of carbon, of which, among the last pro- ducts of transformation, a fourth part is given off in the form of uric acid. 5. This proposition must be received as an undeniable truth, when we reflect on the developement of the young animal in the egg of a fowl. The egg can be shown to contain no other nitrogenized compound ex- cept albumen. The albumen of the yolk is identical with that of the white; (23) the yolk contains, besides, only a yellow fat, in which cholesterine and iron may be detected. Yet we see in the process of incubation, during which no food and no foreign matter, except the oxygen of the air, is introduced, or can take part in the developement of the animal, that out of the albumen, feathers, claws, globules of the blood, fibrine, mem- brane and cellular tissue, arteries and veins, are produced. The fat of the yolk may have contributed, to a certain extent, to the formation of the nerves and brain ; but the carbon of this fat cannot have been em- ployed to produce the organized tissues in which vitality resides, because the albumen, of the white and of the yolk already con- tains, for the quantity of nitrogen present, exactly the proportion of carbon required for the formation of these tissues. 6. The true starting-point for all the tissues is, consequently, albumen; all ni- trogenized articles of food, whether de- rived from the animal or from the vegeta- ble kingdom, are converted into albumen before they can take part in the process of nutrition. All the food consumed by an animal be- comes in the stomach soluble, and capable of entering into the circulation. In the pro- cess by which this solution is effected, only one fluid, besides the oxygen of the air, takes a part ; it is that which is secreted by the lining membrane of the stomach. The most decisive experiments of physio- logists have shown that the process of chymification is independent of the vital force ; that it takes place in virtue of a purely chemical action, exactly similar to those processes of decomposition or transforma- tion which are known as putrefaction, fer- mentation or decay (eremacausis). 7. When expressed in the simplest form, fermentation, or putrefaction, may be de- scribed as a process of transformation that is, a new arrangement of the elementary particles, or atoms, of a compound, yielding two or more new groups or compounds, and caused by contact with other substances, the elementary particles of which are them- selves in a state of transformation or decom- position. It is a communication, or an im- parting of a state of motion, which the atoms of a body in a state of motion are ca- pable of producing- in other bodies, whose elementary particles are held together only by a feeble attraction. 8. Thus the clear gastric juice contains a substance in a state of transformation, by the contact of which with those constituents of the food which, by themselves, are in- soluble in water, the latter acquire, in virtue of a new grouping of their atoms, the pro- 38 ANIMAL CHEMISTRY. perty of dissolving in that fluid. During digestion, the gastric juice, when separated, is found to contain a free mineral acid, the presence of which checks all further change. That the food is rendered soluble quite inde- pendently of the vitality of the digestive organs has been proved by a number of the mosi beautiful experiments. Food, enclosed in perforated metallic tubes, so that it could not come into contact with the stomach, was found to disappear as rapidly, and to be as perfectly digested, as if the covering had been absent; and fresh gastric juice, out of the body, when boiled white of egg, or mus- cular fibre, were kept in contact with it for a time at the temperature of the body, caused these substances to lose the solid form and to dissolve in the liquid. 9. It can hardly be doubted that the sub- stance which is present in the gastric juice in a state of change is a product of the trans- formation of the stomach itself. No sub- stances possess, in so high a degree as those arising from the progressive decomposition of the tissues containing gelatine or chon- drine, the property of exciting a change in the arrangement of the elements of other compounds. When the lining membrane of the stomach of any animal, as, for ex- ample, that of the calf, is cleaned by con- tinued washing with water, it produces no effect whatever, if brought into contact with a solution of sugar, with milk or other sub- stances. But if the same membrane be ex- posed for some time to the air, or dried, and then placed in contact with such substances, the sugar is changed, according to the state of decomposition of the animal matter, either into lactic acid, into mannite and mu- cilage, or into alcohol and carbonic acid j while milk is instantly coagulated. An or- dinary animal bladder retains, when dry, all its properties unchanged; but when ex- posed to air and moisture, it undergoes a change not indicated by any obvious exter- nal signs. If, in this state, it be placed in a solution of sugar of milk, that substance is quickly changed into lactic acid. 10. The fresh lining membrane of the stomach of a calf, digested with weak mu- riatic acid, gives to this fluid no power of dissolving boiled flesh or coagulated white of egg. But if previously allowed to dry, or if left for a time in water, it then yields, to water acidulated with muriatic acid, a substance in minute quantity, the decompo- sition of which is already commenced, and is completed in the solution. If coagulated albumen be placed in this solution, the state of decomposition is communicated to it, first at the edges, which become translucent, pass into a mucilage, and finally dissolve. The same change gradually affects the whole mass, and at last it is entirely dis- solved, with the exception of fatty particles, which render the solution turbid. Oxygen is conveyed to every part of the body by the arterial blood ; moisture is every where pre- sent; and thus we have united the chief conditions of all transformations in the ani- mal body. Thus, as in the germination of seeds, the presence of a body in a state of decomposi- tion or transformation, which has been called diastase, effects the solution of the starch that is, its conversion into sugar ; so, a product of the metamorphosis of the substance of the stomach, being itself in a state of metamorphosis which is completed in the stomach, effects the dissolution of all such parts of the food as are capable of as- suming a soluble form. In certain diseases, there are produced from the starch, sugar, &.C., of the food, lactic acid and mucilage. (24.) These are the very same products which we can produce out of sugar by means of membrane in a state of decompo- sition out of the body ; but in a normal state of health, no lactic acid is formed in the stomach. 11. The property possessed by many sub- stances, such as starch and the varieties of sugar, by contact with animal substances in a state of decomposition, to pass into lactic acid, has induced physiologists, without farther inquiry, to assume the fact of the production of lactic acid during digestion and the power which this acid has of dis- solving phosphate of lime has led them to ascribe to it the character of a general sol* vent. But neither Prout nor Braconnot could detect lactic acid in the gastric juice ; and even Lehmann (see his " Lehrbuch der Physiologischen Chemie," torn. i. p. 285) obtained from the gastric juice of a cat only microscopic crystals, which he took for lac- tate of zinc, although their chemical cha- racter could not be ascertained. The pre- sence of free muriatic acid in the gastric juice, first observed by Prout, has been con- firmed by all those chemists who have ex- amined that fluid since. This muriatic acid is obviously derived from common salt, the soda of which plays a very decided part in the conversion of fibrine and caseine into blood. Muriatic acid yields to no other acid in the power of dissolving bone earth, and even acetic acid, in this respect, is equal to lactic acid. There is consequently no proof of the necessity of lactic acid in the diges- tive process ; and we know with certainty, that in artificial digestion it is not formed. Berzelius indeed has found lactic acid in the blood and flesh of animals; but when his experiments were made, chemists were ignorant of the extraordinary facility and rapidity with which this acid is formed from a number of substances containing its elements, when in contact with animal matter. In the gastric juice of a dog, Braconnot found, along with free muriatic acid, distinct traces of a salt of iron, which he at first held to be an accidental admixture. But in the gastric juice of a second dog, collected with the utmost care, the iron was agaiu found. (Ann. de Ch. et de Ph. lix. p. 249.) NITROGEN EXHALED FROM THE LUNGS. 39 This occurrence of iron is full of signifi- cance in regard to the formation of the blood. \~2. In the action of the gastric juice on the food, no other element takes a share, except the oxygen of the atmosphere and the elements of water. This oxygen is in- troduced directly into the stomach. During the mastication of the food,, there is secreted into the mouth from organs specially des- tined to this function, a fluid, the saliva, which possesses the remarkable property of enclosing air in the shape of froth, in a far higher degree than even soapsuds. This nir, by means of the saliva, reaches the sto- mach with the food, and there its oxygen enters into combination, while its nitrogen is given out through the skin and lungs. The longer digestion continues, that is, the greater resistance offered to the solvent ac- tion by the food, the more saliva, and con- sequently the more air enters the stomach. Rumination, in certain graminivorous ani- mals, has plainly for one object a renewed and repeated introduction of oxygen ; for a more minute mechanical division of the food only shortens the time required for solution." The unequal quantities of air which reach the stomach with the saliva in different classes of animals explain the accurate ob- servations made by physiologists, who have established beyond all doubt the fact, that animals give out pure nitrogen through the skin and lungs, in variable quantity. This fact is so much the more important, as it furnishes the most decisive proof, that the nitrogen of the air is applied to no use in the animal economy. The fact that nitrogen is given out by the skin and lungs, is explained by the property which animal membranes possess of allow- ing all gases to permeate them, a properly which can be shown to exist by the most simple experiments. A bladder, filled with carbonic acid, nitrogen, or hydrogen gas, if tightly closed and suspended in the air, loses in 24 hours the whole of the enclosed gas ; by a kind of exchange, it passes outwards into the atmosphere, while its place is occu- pied by atmospherical air. A portion of intestine, a stomach, or a piece of skin or membrane, acts preciselv as the bladder, if filled with any gas. This permeability to gases is a mechanical property, common to all animal tissues ; and it is found in the some degree in the living as in the dead tissue. It is known that in cases of wounds of the lungs a peculiar condition is produced, in which, by the act of inspiration, not only oxygen but atmospherical air, with its whole amount (4ths) of nitrogen, penetrates into the cells of the lungs. This air is carried by the circulation to every part of the body, so that every part is inflated or puffed up with the air, as with water in dropsy. This state ceases, without pain, as soon as the entrance of the air through the wound is stopped. There can be no doubt that the oxygen of the air, thus accumulated in the cellular tissue, enters into combination, while its nitrogen is expired through the skin and lunsrs. Moreover, it is well known that in many graminivorous animals, when the digestive organs have been overloaded with fresh juicy vegetables, these substances undergo in the stomach the same decomposition as they would at the same temperature out of the body. They pass into fermentation and putrefaction, whereby so great a quantity of carbonic acid gas and of inflammable gas is generated, that these organs are enor- mously distended, sometimes even to burst- ing. From the structure of their stomach or stomachs, these gases cannot escape through the resophagus ; but in the course of a few hours, the distended body of the animal be- comes less swollen, and at the end of twenty- four hours no trace of the gases is left. (25.) Finally, if we consider the fatal accidents which so frequently occur in wine countries from the drinking of what is called feather- white wine (derfederweisse Wtin^ we can no longer doubt that gases of every kind, whether soluble or insoluble in water, pos- sess the property of permeating animal tis- sues, as water penetrates unsized paper. This poisonous wine is wine still in a state of fermentation, which is increased by the heat of the stomach. The carbonic acid gas which is disengaged penetrates through the parietes of the stomach, through the dia- phragm, and through all the intervening membranes, into the air-cells of the lungs, out of which it displaces the atmospherical air. The patient dies with all the symptoms of asphyxia caused by an irrespirable gas ; and the surest proof of the presence of the carbonic acid in the lungs is the fact, that the inhalation of ammonia (which combines with it) is recognized as the best antidote against this kind of poisoning. The carbonic acid of effervescing wines and of soda-water, when taken into the sto- mach, or of water saturated with this gas, ad- ministered in the form of enema, is given out again through the skin and lungs j and this is equally true of the nitrogen which is in- troduced' into the stomach with the food in the saliva. No doubt a part of these gases may enter the venous circulation through the absorb- ent and lymphatic vessels, and thus reach the lungs, where they are exhaled ; but the presence of membranes offers not the slight- est obstacle to their passing directly into the cavity of the chest. It is, in fact, difficult to suppose that the absorbents and lympha- tics have any peculiar tendency to absorb air, nitrogen or hydrogen, and convey these gases into the circulation, since the intestines, the stomach, and all spaces in the body not filled with solid or liquid matters, contain gases, which only quit their position when their volume exceeds a certain point, and which, consequently, are not absorbed. 40 ANIMAL CHEMISTRY. More especially in reference to nitrogen, we must suppose that it is removed from the stomach by some more direct means, and not by the blood, which fluid must already, in passing through the lungs, have become saturated with that gas, that is, must have absorbed a quantity of it, proportioned to its solvent power, like any other liquid. By the respiratory motions, all the gases which rill the otherwise empty spaces of the body are urged towards the chest ; for by the motion of the diaphragm and the expansion of the chest a partial vacuum is produced, in con- sequence of which air is forced into the chest from all sides by the atmospheric pres- sure. The equilibrum is, no doubt, restored, for the most part, through the windpipe, but all the gases in the body must, nevertheless, receive an impulse towards the chest. In birds and tortoises these arrangements are reversed. If we assume that a man intro- duces into the stomach in each minute only th of a cubic inch of air with the saliva, this makes in eighteen hours 135 cubic inches j and if th be deducted as oxygen, there will still remain 108 cubic inches of nitrogen, which occupy the space of 3 Ibs. of water. Now whatever may be the actual amount of the nitrogen thus swallowed, it is certain that the whole of it is given out again by the mouth, nose, and skin ; and when we consider the very large quantity of nitrogen found in the intestines of exe- cuted criminals by Magendie, as well as the entire absence of oxygen in these organs, (26,) we must assume that air, and conse- quently nitrogen, enters the stomach by re- sorption through the skin, and is afterwards exhaled by the lungs. When animals are made to respire in gases containing no nitrogen, more of that gas is exhaled, because in this case the nitrogen within the body acts towards the external space as if the latter were a vacuum. (See Graham" On the Diffusion of Gases.") The differences in the amount of expired nitrogen in different classes of animals are thus easily explained ; the herbivora swal- low with the saliva more air than the carni- vora; they expire more nitrogen than the latter, less when fasting than immediately after taking food. 13. In me same way as muscular fibre, when separated from the body, communi- cates the state of decomposition existing in its elements to the peroxide of hydrogen, so a certain product, arising by means of the vital process, and in consequence of the transposition of the elements of parts of the stomach and of the other digestive organs, while its own metamorphosis is accom- plished in the stomach, acts on the food. The insoluble matters become soluble they are digested. It is certainly remarkable, that hard-boiled white of egg, or fibrine, when rendered so- luble by certain liquids, by organic acids, or weak alkaline solutions, retain all their properties except the solid form (cohesion) without the slightest change. Their ele- mentary molecules, without doubt assume a new arrangement ; they do not, however, separate into two or more groups, but re- main united together. The very same thing occurs in the di- gestive process ; in the normal state, the food only undergoes a change in its state of co- hesion, becoming fluid without any other change of properties. The greatest obstacle to forming a clear conception of the nature of the digestive process, which is "here reckoned among those chemical metamorphoses which have been called fermentation and putrefaction, consists in our involuntary recollection of the phenomena which accompany the fer- mentation of sugar and of animal sub- stances, (putrefaction,) which phenomena we naturally associate with any similar change; but there are numbe&less cases in which a complete chemical metamorphosis of the elements of a compound occurs with- out the smallest disengagement of gas, and it is chiefly these which must be borne in mind, if we would acquire a clear and accu- rate idea of the chemical notion or concep- tion of the digestive process. All substances which can arrest the phe- nomena of fermentation and putrefaction in liquids, also arrest digestion when taken into the stomach. The action of the empyreu- matic matters in coffee and tobacco smoke, of creosote, of mercurials, &c., &.C., is on this account worthy of peculiar attention with reference to dietetics. The identity in composition of the chief constituents of blood and of the nitrogenized constituents of vegetable food has certainly furnished, in an unexpected manner, an explanation of the fact that putrefying blood, white of egg, flesh, and cheese produce the same effects in a solution of sugar as yeast or ferment; that sugar, in contact with these substances, according to the particular stnge of decomposition in which the putrefying matters may be, yields, at one time, alcohol and carbonic acid ; at another, lactic acid, mannite, and mucilage. The explanation is simply this, that ferment, or yeast, is nothing but vegetable fibrine, albumen, or caseine in a state of decomposition, these substances having the same composition with the constituents of flesh, blood, or cheese. The putrefaction of these animal matters is a process identical with the meta- morphosis of the vegetable matters identical with them ; it is a separation or splitting up into new and less complex compounds. And if we consider the transformation of the elements of the animal body (the waste of matter in animals) as a chemical process which goes on under the influence of the vital force, then the putrefaction of animal matters out of the body is a division into simpler compounds, in which the vital force takes no share. The action in both cases is the same, only the products differ. The practice of medicine has furnished the COMPOSITION OF FIBRINE &c. 41 most beautiful and interesting observations on the action of empyreumatic substances, such as wood, vinegar, creosote, &c., on malignant wounds and ulcers. In such morbid phenomena two actions are going on together; one metamorphosis, which strives to complete itself under the influence of the vital force, and another, independent of that force. The latter is a chemical pro- cess, which is entirely suppressed or arrested by empyreumatic substances ; and this effect is precisely opposed to the poisonous influ- ence exercised on the organism by putrefy- ing blood when introduced into a fresh wound. 14. The formula C H 36 NO 14 * is that which most accurately expresses the com- position of proteine, or the relative propor- tions of the organic elements in the blood, as ascertained by analysis. Albumen, fibrine, and caseine contain proteine; caseine con- tains, besides, sulphur, but no phosphorus ; albumen and fibrine contain both these sub- stances chemically combined the former more sulphur than the latter. We cannot directly ascertain in what form the phos- phorus exists. But we have decided proof that the sulphur cannot be in the oxidized state. All these substances, when heated with a moderately strong solution of potash, yield the sulphur which we find in the solu- tion as sulphuret of potassium ; and on the addition of an acid it is given off as sul- phuretted hydrogen. When pure fibrine or ordinary albumen is dissolved in a weak solution of potash, and acetate of lead is added to the solution, in such proportion that the whole of the oxide of lead remains dissolved in the potash, the mixture, if heated to the boiling point, becomes black like ink, and sulphuret of lead is deposited as a fine black powder. It is extremely probable, that by the action of the alkali the sulphur is removed as sulphuretted hydrogen, the phosphorus as phosphoric or phosphorus acid. Since, in this case, sulphur and phosphorus are eliminated on the one hand, and oxygen and hydrogen on the other, it might be con- cluded that fibrine and albumen, when analyzed with their sulphur and phosphorus, would yield a larger proportion of oxygen and hydrogen than is found in proteine. But this cannot be shown in the analysis; for fibrine, for example, has been found to con- tain 0-36 per cent, of sulphur. Assuming, then, that this sulphur is eliminated by the alkali in combination with hydrogen, pro- teine would yield 0'0225 per cent, less hy- drogen than fibrine; instead of the mean amount of 7'062 per cent, of hydrogen, the proteine should yield 7'04 per cent. In like manner, by the elimination of the phos- phorus in combination with oxygen, the amount of oxygen in fibrine would be re- duced from 22-715 22-00 per cent, to 22-5 * For the method of converting this and other formula into proportions per cent., see Appendix. 6 21'8 per cent, in proteine. But the limits of error in our analyses are, on an average, beyond -j^th per cent, in the hydrogen, and beyond T 4 a ths per cent, in the oxygen ; while in the supposed case the difference in the hydrogen would not be greater than 'jth per cent. Finally, if we reflect, thai <\\e elimination of oxygen and hydrogen with the sulphur | and phosphorus does not exclude the addi- tion of the elements of water, and if we as- sume that fibrine and albumen, in passing into proteine, do combine with a certain quantity of water, an occurrence which is highly probable, we shall see that there is no probability that the ultimate analysis of these compounds shall ever enable us to de- cide such questions, or to fix the chemical view of the relation of proteine to albumen, fibrine, or caseine, farther than has been done above. Some have endeavoured to prove the ex- istence of unoxidized phosphorus in albumen and fibrine from the formation of sulphuret of potassium when they are acted on by potash, supposing the oxygen of the potash to have formed phosphoric acid with the phosphorus; but caseine, which contains j no phosphorus, yields sulphuret of potas- sium, just like the other substances; and here its formation cannot be accounted for, unless we admit the previous production of sulphuretted hydrogen. In the mere boiling of flesh, for the purpose of making soup, sulphuretted hydrogen, as Chevreul has shown, is disengaged. Moreover, the proportion of sulphur, for the same amount of phosphorus, is not the same in fibrine and albumen, from which no other conclusion can be drawn, but that the formation of sulphuret of potassium has no relation to the presence of phosphorus. Sul- phuret of potassium is formed from caseine, which is not supposed to contain any un- combined phosphorus; and it is formed, also, from albumen, which contains only half as much phosphorus as fibrine. Every attempt to give the true absolute amount of the atoms in fibrine and albumen in a rational formula, in which the sulphur and phosphorus are taken, not in fractions, but in entire equivalents, must be fruitless, because we are absolutely unable to deter- mine with perfect accuracy the exceedingly minute quantities of sulphur and phosphorus in such compounds; and because a variation | in the sulphur or phosphorus, smaller in extent than the usual limit of errors of ob- servation, will affect the number of atoms of carbon, hydrogen, or oxygen to the extent ! of 10 atoms or more. We must be careful not to deceive our- selves in our expectations of what chemical analysis can do. We know, with certainty, that the numbers representing the relative proportions of the organic elements are the same in albumen and fibrine, and hence we conclude that they have the same composi- tion. This conclusion is not affected by the 452 ANIMAL CHEMISTRY. fact, that we do not know the absolute num- ber of the atoms of their elements, which have united to form the compound atom. 15. A formula for proteine is nothing 1 more than the nearest and most exact ex- pression in equivalents, of the result of the best analyses ; it is a fact established so far, free from doubt, and this alone is, for the present, valuable to us. If we reflect, that from the albumen and fibrine of the body all the other tissues are derived, it is perfectly clear that this can only occur in two ways. Either certain elements have been added to, or removed from, their constituent parts. If we now, for example, lock for an ana- lytical expression of the composition of cel- lular tissue, of the tissues yielding gelatine, or tendons, of hair, of horn, ,c., in which the number of atoms of carbon is made in- variably the same as in albumen and fibrine, v/e can then see at the first glance, in what way the proportion of the other elements has been altered; but this includes all that physiology requires in order to obtain an in- sight into the true nature of the formative and nutritive processes in the animal body. From the researches of Mulder and Sche- rer we obtain the following empirical form ulae. Composition of organic tissues. Albumen . . . C 48 N 6 H 36 O 14 -f-P+S* Fibrine . . . C 48 i\ 6 H 36 O 14 - r -P+2S Caseine . . . C 48 N 6 H 36 14 -f S Gelatinous tissues, > ^ Choadrme . . . C 48 N 6 H 40 O 20 Hair, horn. . . C 48 N 7 H 38 O 17 Arterial membrane . C 48 N 6 H 38 16 The composition of these formulae shows, that when proteine passes into chondrine, (the substance of the cartilages of the ribs,) the elements of water, with oxygen, have been added to it; while in the formation of the serous membranes, nitrogen also has entered into combination. If we represent the formula of proteine, C^'HfQ?* by Pr, then nitrogen, hydrogen, and oxygen have been added to it in the form of known compounds, and in the fol- lowing proportions, in forming the gelatinous tissues, hair, horn, arterial membrane, &c. Proteine. Ammonia. Water. Oxygen. Fibrine, Albumen Rr Arterial Membrane Pr . -f2HO. Chondrine . . Pr . +4HO.+2O. Hair, horn . . Pr-f- NH 3 . . +3O. Gelatinous tissues 2Pr-f-3NH 3 -f HO.-f 7O. 17. From this general statement it ap- pears that all the tissues of the body contain, for the same amount of carbon, more oxygen than the constituents of blood. During iheir formation, oxygen, either from the atmo- sphere or from the elements of water, has been added to the elements of protpine. In * The quantities of sulphur and phosphorus here expressed by S and P are not equivalents, hair and gelatinous membrane we observe, farther, an excess of nitrogen and hydrogen, and that in the proportions to form ammonia. Chemists are not yet agreed on the ques- tion, in what manner the elements of sul- phate of potash are arranged ; it would therefore be going too far, were they to pronounce arterial membrane a hydrate of proteine, chondrine a hydrated oxide of pro- teine, and hair and membranes compounds of ammonia with oxides of proteine. The above formula express with preci- sion the differences of composition in the chief constituents of the animal body; they show, that for the same amount of carbon the proportion of the other elements varies, and how much more oxygen or nitrogen one compound contains than another. 18. By means of these formulae we can trace the production of the different com- pounds from the constituents of blood; but the explanation of their production may take two forms, and we have to decide which of these comes nearest to the truth. For the same amount of carbon, mem- branes and the tissues which yield gelatine contain more nitrogen, oxygen, and hydro- gen than proteine. It is conceivable that they are formed from albumen by the addi- tion of oxygen, of the elements of water, and of those of ammonia, accompanied by the separation of sulphur and phosphorus; at all events, their composition is entirely different from that of the chief constituents of blood. The action of caustic alkalies on the tis- sues yielding gelatine shows distinctly that they no longer contain proteine ; that sub- stance cannot in any way be obtained from them; and all the products formed by the action of alkalies on them differ entirely from those produced by the compounds of proteine in the same circumstances. Whe- ther proteine exist, ready formed, in fibrine, albumen, and caseine, or not, it is certain that their elements, under the influence of the alkali, arrange themselves so as to form proteine; but this property is wanting in the elements of the tissues which yield gelatine. The other, and perhaps the more proba- ble explanation of the production of these tissues from proteine, is that which makes it dependent on a separation of carbon. If we assume the nitrogen of proteine to remain entire in the gelatinous tissue, then the composition of the latter calculated on 6 equivalents of nitrogen, would be repre- sented by the formula, CWITO 14 . This formula approaches most closely to the analysis of Scherer, although it is not an exact expression of his results. A formula corresponding more perfectly to the analysis, is C^J^H^O 12 ; or calculated according to Mulder's analysis, The formula C 52 N8H 40 20 , adopted by Mul- der, gives, when reduced to 100 parts, too little but only give the relative proportions of these two j nitrogen to be considered an exact expression of elements to each other, as found by analysis. I his analyses. METAMORPHOSIS OF TISSUES. 43 According to the first formula, carbon and hydrogen have been separated; according to tha two last, a certain proportion of all the elements has been removed 19. We must admit, as the most im- portant result of the study of the composi- tion of gelatinous tissue, and as a point un- deniably established, that, although formed from compounds of proteine, it no longer belongs to the series of the compounds of proteine. Its chemical characters and com- position justify this conclusion. No fact is as yet opposed to the law, de- duced from observation, that nature has ex- clusively destined compounds of proteine for the production of blood. No substance analogous to the tissues Yielding gelatine is found in vegetables. The gelatinous substance is not a compound of proteine ; it contains no sulphur, no phos- phorus, and it contains more nitrogen or less carbon than proteine. The compounds of proteine, under the influence of the vital energy of the organs which form the blood, assume a new form, but are not altered in composition; while these organs, as far as our experience reaches, do not possess the power of producing compounds of proteine, by virtue of any influence, out of substances which contain no proteine. Animals which are fed exclusively with gelatine, the most highly nitrogenized element of the food of carnivora, died with the symptoms of starva- tion; in short, the gelatinous tissues are incapable of conversion into blood. But there is no doubt that these tissues are formed from the constituents of the blood ; and we can hardly avoid entertain- ing the supposition, that the fibrine of venous blood, in becoming arterial fibrine, passes through the first stage of conversion into gelatinous tissue. We cannot, with much probability, ascribe to membranes and ten- dons the power of farming themselves out of matters brought by the blood; for how could any matter become a portion of the cellular tissue, for example, by virtue of a force which has as yet no organ ? An al- ready existing cell may possess the power of reproducing or of multiplying itself, but in both cases the presence of a substance iden- tical in composition with cellular tissue is essential. Such matters are formed in the organism, and nothing can be better fitted lor their production than the substance of the cells and membranes which exist in ani- mal food, and become soluble in the stomach during digestion, or which are taken by man in a soluble form. 20. In the following pages I offer to the reader an attempt to develope analytically the principal metamorphoses which occur in the animal body; and, to preclude all misapprehension, I do this with a distinct protest against all conclusions and deduc- tions which may now or at any subsequent period be derived from it in opposition to the views developed in the preceding part of this work, with which it has no manner of connexion. The results here to be de- scribed have surprised me no less than they will others, and have excited in my mind I the same doubts as others will conceive; j but they are not the creations of fancy, and I 1 give them because I entertain the deep , conviction that the method which has led to them is the only one by which we can hope I to acquire insight into the nature of the organic processes. The numberless qualitative investigations of animal matters which are made are equally worthless for physiology and for chemistry., so long as they are not instituted with a well defined object, or to answer a question clearly put. If we take the letters of a sentence which we wish to decipher, and place them in a line, we advance not a step towards the dis- covery of their meaning. To resolve an enigma, we must have a perfectly clear con- ception of the problem. There are many ways to the highest pinnacle of *i mountain ; but those only can hope to reach it who keep the summit constantly in view. All our labour and all our efforts, if we strive to attain it through a morass, only serve to cover us more completely with mud; our progress is impeded by difficulties of our own creation, and at last even the greatest strength must give way when so absurdly wasted. 21. If it be true that all parts of the body are formed and developed from the blood or the constituents of the blood, that the exist- ing organs at every moment of life are trans- formed into new compounds under the in- fluence of the oxygen introduced in the blood, then the animal secretions must of necessity contain the products of the meta- morphosis of the tissues. 22. If it be further true, that the urine contains those products of metamorphosis which contain the most nitrogen, and the bile those which are richest in carbon, from all the tissues which in the vital process have been transformed into unorganized com pounds, it is clear that the elements of the bile and of the urine, added together, must be equal in the relative proportion of these elements to the composition of the blood. 23. The organs are formed from the blood, and contain the elements of the blood ; they become transformed into new compounds, with the addition only of oxygen and of water. Hence the relative proportion of carbon and nitrogen must be the same as in the blood. If then we subtract from the composition of blood the elements of the urine, then the remainder, deducting the oxygen and water which have been added, must give the com- position of the bile. Or if from the elements of the blood, we subtract the elements of the bile, the remain- der must give the composition of urate of ammonia, or of urea and carbonic acid. It will surely appear remarkable that this manner of viewing the subject has led to the | true formula of bile, or, to speak more accu- 44 ANIMAL CHEMISTRY. rately, to the most correct empirical expres- sion of its composition; and has furnished the key to its metamorphoses, under the in- fluence of acids and alkalies, which had pre- viously been sought for in vain. 24. When fresh drawn blood is made to tnckle over a plate of silver, heated to 140, it dries to a red, varnish-like matter, easily reduced to powder. Muscular flesh, free from fat, if dried first in a gentle heat, and then at 212, yields a brown, pulverizable mass. The analyses of Play fair and Boeck- mann (28) give for flesh (fibrine, albumen, cellular tissue, and nerves) and for blood, as the most exact expression of their numerical results, one and the same formula, namely, C 48 N 6 H 39 O 15 . This may be called the em- pirical formula of blood. 25. The chief constituent of bile, accord- ing to the researches of Demarc.ay, is a compound, analogous to soaps, of soda with a peculiar substance, which has been named choleic acid. This acid is obtained in com- bination with oxide of lead, when bile, puri- fied by means of alcohol from all matters insoluble in that menstruum, is mixed with acetate of lead. Choleic acid is resolved, by the action of muriatic acid, into ammonia, taurine, and a new acid, choloidic acid, which contains no nitrogen. When boiled with caustic potash, choleic acid is resolved into carbonic acid, ammonia, and another new acid, cholic acid (distinct from the cholic acid of Gmelin.) Now it is clear that the true formula of choleic acid must include the analytical ex- pression of these modes of decomposition ; in other words, that it must enable us to show that the composition of the products derived from it is related in a clear and simple manner, to the composition of the acid itself. This is the only satisfactory test of a formula ; and the analytical expression thus obtained loses nothing of its truth or value, if it should appear, as the researches of Berzelius seem to show, that choleic and choloidic acids are mixtures of different compounds ; for the relative proportions of the elements cannot in any way be altered by this circumstance. 26. In order to develope the metamor- phoses which choleic acid suffers under the influence of acids and alkalies, the following formula alone can be adopted as the empiri- cal expression of the results of its analysis, Formula of choleic acid: C'WH^O 22 . (29) I repeat, that this formula may express the composition of one, or of two or more compounds ; no matter of how many com- pounds the so-called choleic acid may be made up, the above formula represents the relative proportions of all their elements taken together. If now we subtract from the elements of choleic acid, the products formed by the action of muriatic acid, namely, ammonia and taurine, we obtain the empirical formula of choloidic acid. Thus from the Formula of choleic acid Substract 1 at. taurine C 4 NH 7 10 ? 1 eq. ammonia NH 3 $ There remains the for- mula of choloidic acid ...... C 72 27. Again, if from the formula of choleic acid we subtract the elements of urea and 2 atoms of water (=2 eq. carbonic acid and 2 eq. ammonia,) there will remain the formula and composition of cholic acid. Thus : from the Formula of choleic acid =C 76 N 2 H 66 22 Substract 2 eq. car. acid =C 2 O 4 > 2 eq. ammonia = N 2 H 6 5 =C 2 N 2 H 6 O 4 Remains the formula of cholic acid =C 74 H 60 18 (31.) When we consider the very close coinci- dence between these formula and the actual results of analysis (see Appendix, 29, 30, 31,) it is scarcely possible to doubt that the formula above adopted for choleic acid ex- presses, as accurately as is to be expected in the analysis of such compounds, the rela- tive proportion of its elements, no matter in how many different forms they may be united to produce that acid. 28. Let us now add the half of the num- bers which represent the formula of choleic acid, to the elements of the urine of ser- pents that is, to neutral urate of ammonia, as follows : i the formula of choleic acid CN'H0" Add to this 1 eq. uric acid =C 10 N 4 H 4 O 6 ? 1 eq. ammonia = NH 3 $ The sum is =C 48 N 6 H 40 O 17 29. But this last formula expresses the composition of blood, with the addition of 1 eq. oxygen, and 1 eq. water. Formula of blood .... C 48 N 6 H 39 O^ 1 eq. water =HO > _ H 1 O 2 1 eq. oxygen == O j ' The sum is , , = 30. If, moreover, we add to the elements of proteine those of 3 eq. water, we obtain, with the exception 1 eq. hydrogen, exactly the same formula. Formula of proteine . . ==C 48 N 6 H 36 14 Add 3 eq. of water . . . = H 3 O 3 The sum is differing only by 1 eq. of hydrogen from the formula above obtained by adding to ether choleic acid and urate of ammonia. 31. If, then, we consider choleic acid and urate of ammonia the products of the trans- formation of muscular fibre, since no other tissue in the body contains proteine (for albumen passes into tissues, without our being able to say, that in the vital process it is directly resolved into choleic acid, and urate of ammonia,) there exist in fibrine, with the addition of the elements of water, all the elements essential to this metamor URIC ACID AND UREA. 45 phosis ; and, except the sulphur and phos- phorus, both of which are probably oxidized, no element is separated. This form of metamorphosis is applicable to the vital transformations in the lower classes of amphibia, and perhaps in worms and insects. In the higher classes of ani- mals the uric acid disappears in the urine, and is replaced by urea. The disappearance of uric acid and the pro- duction of urea plainly stand in a very close relation to the amount of oxygen absorbed in respiration, and to th^ quantity of water con- sumed by different animals in a given time. When uric acid is subjected to the action of oxygen, it is first resolved, as is well known, into alloxan and urea. (32.) A new supply of oxygen acting on the alloxan causes it to resolve itself either into oxalic acid and urea, into oxaluric and parabanic acids, (33,) or into carbonic acid and urea. 32. In the so-called mulberry calculi we find oxalate of lime, in other calculi urate of ammonia, and always in persons, in whom, from want of exercise and labour, or from other causes, the supply of oxygen has been diminished. Calculi containing uric acid or oxalic acid are never found in phthisical patients; and it is a common occurrence in France, among patients suf- fering from calculous complaints, that when they go to the country, where they take more exercise, the compounds of uric acid, which were deposited in the bladder during their residence in town, are succeeded by oxalates (mulberry calculus,) in consequence of the increased supply of oxygen. With a still greater supply of oxygen they would have yielded, in healthy subjects, only the last product of the oxidation of uric acid, namely, carbonic acid and urea. An erroneous interpretation of the unde*- niable fact that all substances incapable of farther use in the organism are separated by the kidneys and expelled from the body in the urine, altered or unaltered, has led prac- tical medical men to the idea, that the food, and especially nitrogenized food, may have a direct influence on the formation of urinary calculi. There are no reasons which sup- port this opinion, while those opposed to it are innumerable. It is possible that there may be taken, in the food, a number of mat- ters changed by the culinary art, which, as Deing no longer adapted to the formation of blood, are expelled in the urine, more or less altered by the respiratory process. But roasting and boiling alter in no way the composition of animal food. (34.) Boiled and roasted flesh is converted at once into blood ; while the uric acid and uiea are derived from the metamorphosed tissues. The quantity of these products increases with the rapidity of transformation in a given time, but bears no proportion to the amount of food taken in the same period. In a starving man who is in any way com- pelled to undergo severe and continued ex- ertion, more urea is secreted than in the most highly fed individual, if in a state of rest. In fevers and during rapid emaciation the urine contains more urea than in a state of health. (Prout.) 33. In the same way, therefore, as the hippuric acid, present m the urine of the horse when at rest, is converted into ben- zoate of ammonia and carbonic acid as soon as the animal is compelled to labour, so the uric acid disappears in the urine of man, when he receives, through the skin and lungs, a quantity of oxygen sufficient to oxidize the products of the transformation of the tissues. The use of wine and fat, which are only so far altered in the organ- ism that they combine with oxygen, has a marked influence on the formation of uric acid. The urine, after fat food has been taken, is turbid, and deposits minute crystals of uric acid. (Prout.) The same thing is observed after the use of wines in which the alkali necessary to retain the uric acid in solution is wanting, but never from the use of Rhenish wines, which contain so much tartar. In animals which drink much water, bjr means of which the sparingly soluble uric acid is kept dissolved, so that the inspired oxygen can act on it, no uric acid is found in the urine, but only urea. In birds, which seldom drink, uric acid predominates. If to one atom of uric acid we add 6 atoms of oxygen and 4 atoms of water, it resolves itself into urea and carbonic acid: 1 atom uric acid 4 atoms water > 6 atoms oxygen > H 4 O 10 C 10 N 4 H 8 O 18 C 4 N 4 H 8 O 4 C 6 O* C 2 atoms urea 1 6 atoms carbonic acid 34. The urine of the herbivora contains no uric acid, but ammonia, urea, and hippuric or benzoic acid. By the addition of 9 atoms of oxygen to the empirical formula of their blood multiplied by 5, we obtain the ele- ments of 6 at. of hippuric acid, 9 at. of urea, 3 at. of choleic acid, 3 at. of water, and 3 at. of ammonia; or, if we suppose 45 atoms of oxygen to be added to the blood during its metamorphoses, then we obtain 6 at. of benzoic acid, 13 at. of urea, 3 at. of choleic acid, 15 at. of carbonic acid, and 12 at. of water. 5(C 48 X 6 H M O 1S )4-O 9 = f 6 atoms hippuric acid, 6 (C 18 N H 8 O 5 ) = C 108 X 6 H^O 30 1 9 atoms urea . . . 9 (C 2 N 2 H 4 O 2 ) = C 18 N I8 H 36 O 18 3 atoms choleic acid . 3 (C^N H 38 O n ) = C 1M X 3 H"O 33 3 atoms ammonia . 3 ( N H 3 ) N 3 H 9 3 atoms water . . 3 ( _ I? 8 O 3 ) = H*O 3 The sum is , 46 ANIMAL CHEMISTRY. Or 5 (C 48 N 6 H 39 O 15 ) + O 43 = c 2l N 30 H 195 O 120 6 atoms benzole acid, 6 (C 14 H 5 O 3 ) = C 84 H 30 O 18 27| 2 atoms urea . . 27 (C NH 2 O ) = C 27 N^H^O 27 3 atoms choleic acid 3 (C 38 NH 33 O n ) = C 114 N 3 H^O 33 15 atoms carbonic acid 15 (C O 2 ) = C 15 O 30 12 atoms water . . 12 ( H O ) = H 12 O 12 The sum is 35. Lastly, let us follow tho metamor- phosis of the tissues in the fostal calf, con- sidering- the proteine furnished in the blood of the mother as the substance which under- goes or has undergone a transformation; it will appear that 2 at. of proteine without the addition of oxygen or any other foreign element, except 2 at. of water, contain the elements of 6 at. of allantoine and 1 at. of choloidic acid (meconium?) 2 atoms proteine = 2 (C 48 N 6 H 36 O 14 ) -f 2 atoms water = 2HO 6 atoms allantoine, 6 (C 4 N 2 H 3 O 3 ) = C 24 N 12 H 18 O 18 1 atom choloidic acid = C 72 H 56 O 12 36. But the elements of the six atoms of I exactly to the elements of 2 at. of uric acid, allantoine in the last equation correspond | 2 at. of urea, and 2 at. of water. ("2 atoms uric acid C 20 N 8 H 8 O 12 6 atoms of allantoine = C^N 12 !! 1 ^ 18 = < 2 atoms urea C 4 N 4 H 8 O 4 2 atoms water H 2 O 2 The relations of allantoine, which is found in the urine of the fetal calf, to the nitro- genized constituents of the urine in animals which respire, are, as may be seen by com- paring the above formulae, such as cannot be overlooked or doubted. Allantoine con- tains the elements of uric acid and urea that is, of the nitrogenized products of the transformation of the compounds of proteine. 37. Further, if to the formula of proteine, multiplied by 3, we add the elements of 4 at. of water, and if we deduct from the sum of all the elements half of the elements of choloidic acid, there remains a formula which expresses very nearly the composi- tion of gelatine. From 3 (CWH^O 14 ) -h 4 HO ... Subtract atom choloidic acid C 144 N 18 H 112 O 46 C 86 H 28 O 6 There remain . 38. Subtracting from this formula of gela- tine the elements of 2 at. of proteine, there remain the elements of urea, uric acid, and water, or of 3 at. of allantoine and 3 at. of water. Thus Formula of gelatine (Mulder) Subtract 2 atoms proteine . G 96 N 12 H 72 O 28 There remain atom uric acid C 10 N 4 H 4 O 6 ~) C C 2 N 2 H 4 O 2 > = -< H 4 O 4 3 atom urea atoms water 39. The numerical proportions calculated from the above formula differ from those actually obtained in the analyses of Mulder and Sherer in this, that the latter indicate somewhat less of nitrogen in gelatine; but if we assume the formula to be correct, it then appears, from the statement just given, that the elements of two atoms of proteine, plus the nitrogenized products of the trans- formation of a third atom of proteine (uric acid and urea) and water; or three atoms of proteine, minus the elements of a com- pound containing no nitrogen, which ac- tually occurs as one of the products of the transformation of choleic acid, yield in both to the r e must, and to the considerations arising from them, no 3 atoms allantoine C 12 N 6 H 9 O 9 3 atoms water . H 3 O 3 more importance than justly belongs to them. I would constantly remind the reader that their use is to serve as points of con- nexion, which may enable us to acquire more accurate views as to the production and decomposition of those compounds which form the animal tissues. They are the first attempts to discover the path which we must follow in order to attain the object of our researches ; and this object, the goal we strive to reach, is, and must be, at- tainable. The experience of all those who have oc- cupied themselves with researches into na- tural phenomena leads to this general result, that these phenomena are caused or pro- duced, by means far more simple than those previously supposed, or than we even now imagine ; and it is precisely their simplicity ORIGIN OF THE BILE. 47 which should most powerfully excite our wonder and admiration. Gelatinous tissue is formed from blood, from compounds of proteine. It may be produced by the addition, to the elements of proteine, of allantoine and water, or of wa- ter, urea, and uric acid ; or by the separation from the elements of proteine of a com- pound containing no nitrogen. The solution of such problems becomes less difficult, when the problem to be solved, the question to be answered, is matured and clearly put. Every experimental decision of any such question in the negative forms the starting- point of a new question, the solution of which, when obtained, is- the necessary consequence of our having put the first question. 40. In the foregoing sections, no other constituent of the bile, besides choleic acid, has been brought into the calculation ; be- cause it alone is known with certainty to contain nitrogen. Now, if it be admitted that its nitrogen is derived from the meta- morphosed tissues, it is not improbable that the carbon, and other elements which it con- tains, are derived from the same source. There cannot be the smallest doubt, that in the carnivora, the constituents of the urine and the bile are derived from the trans- formation of compounds of proteine; for, except fat, they consume no food but such as contains proteine, or has been formed from that substance. Their food is identical with their blood ; and it is a matter of in- difference which of the two we select as the starting-point of the chemical developement of the vital metamorphoses. There can be no greater contradiction, with regard to the nutritive process, than to suppose that the nitrogen of the food can pass into the urine as urea, without having previously become part of an organized tis- sue; for albumen, the only constituent of blood, which, from its amount, ought to be taken into consideration, suffers not the slightest change in passing through the liver or kidneys ; we find it in every part of the body with the same appearance and the same properties. These organs cannot be adapted for the alteration or decomposition of the substance from which all the other organs of the body are to be formed. 41. From the characters of chyle and lymph, it appears with certainty that the soluble parts of the food or of the chyme acquire the form of albumen. Hard-boiled while of egg, boiled or coagulated fibrin e, which have again become soluble in the stomach, but have lost their coagulability by the action of air or heat, recover these pro- perties by degrees. In the chyle, the acid reaction of the chyme has already passed into the weak alkaline reaction of the blood ; and the chyle, when, after passing through the mesenteric glands, it has reached the tho- racic duct, contains albumen coagulable by heat ; and, when left to itself, deposits fibrine. All the compounds of proteine, absorbed dur- ing the passage of the chyme through tho intestinal canal, take the form of albumen, which, as the results of incubation in the fowl's egg testify, contains the fundamental elements of all organized tissues, with the exception of iron, which is obtained from other sources. Practical medicine has long ago answered the question, what becomes in man of the compounds of proteine taken in excess, what change is undergone by the supera- bundant nitrogenized food? The blood-ves- sels are distended with excess of blood, the other vessels with excess of their fluids, and if the too great supply of food be kept up, and the blood, or other fluids adapted for forming blood, be not applied to their natu- ral purposes, if the soluble matters be not taken up by the proper organs, various gases are disengaged, as in processes of putrefac- faction, the excrements assume an altered quality in colour, smell, &,c. Should the fluids in the absorbent and lymphatic ves- sels undergo a similar decomposition, this is immediately visible in the blood, and the nutritive process then assumes new forms. 42. No one of all these appearances should occur, if the liver and kidneys were capable of effecting the resolution of the superabun- dant compounds of proteine into urea, uric acid, and bile. All the observations which have been made in reference to the influence of nitrogenized food on the composition of the urine have failed entirely to demonstrate the existence of any direct influence of the kind ; for the phenomena are susceptible of another and a far more simple interpretation, if, along with the food, we consider the mode of life and habits of the individuals who have been the subjects of investigation. Gravel and calculus occur in persons who use very little animal food. Concretions of uric acid have never yet been observed in carnivorous mammalia, living in the wild state,* and among nations which live entirely on flesh, deposits of uric acid concretions in the limbs or in the bladder are utterly un- known. 43. That which must be viewed as an undeniable truth in regard to the origin of the bile, or, more accurately speaking, of choleic acid in the carnivora, cannot hold in regard to all the constituents of the bile se- creted by the liver in the herbivora, for with the enormous quantity of bile produced, for example, by the liver of an ox, it is abso- lutely impossible to suppose that all its car- bon is derived from the metamorphosed tissues. Assuming the 59 oz. of dry bile (from 37 Ibs. of fresh bile secreted by an ox) to con- tain the same per centage of nitrogen as cho- leic acid, (3-86 per cent.,) this would amount to nearly 2 oz. of nitrogen; and if this ni- * The occurrence of urate of ammonia in a con cretion found in a dog, which was examined by Lassaigne, is to be doubted, unless Lassaigne ex- tracted it himself from the bladder of the animal. ANIMAL CHEMISTRY. trogen proceed from metamorphosed tissues, then, if all the carbon of these tissues passed into the bile, it would yield, at the utmost, a quantity of bile corresponding to 7-15 oz. of carbon. This is, however, far below the quantity which, according to observation, is secreted in this class of animals. 44. Other substances, besides compounds of proteine, must inevitably take part in the formation of bile in the organism of the herbivora; and these substances can only be the non-nitrogenized constituents of their food. 45. The sugar of bile of Gmelin (picromel or biline of Berzelius,) which Berzelius con- siders as the chief constituent of bile, while Demar9ay assigns that place essentially to choleic acid, burns, when heated in the air, like resin, yields ammoniacal products, and when treated with acids, yields taurine and the products of the decomposition of choleic acid; when acted on by alkalies, it yields ammonia and ckolic acid. At all events, the sugar of bile contains nitrogen, and much less oxygen than starch or sugar, but more oxygen than the oily acids. When, in the metamorphosis of sugar of bile or choleic acid by alkalies, we cause the separation of nitrogen, we obtain a crystallized acid, very similar to the oily acids (cholic acid,) and capable of forming with bases salts, which have the general characters of soaps. Nay, we may even consider the chief con- stituents of the bile, sugar of bile and cho- leic acid, as compounds of oily acids with organic oxides, like the fat oils, and only differing from these in containing no oxide of glycerule. Choleic acid, for example, may be viewed as a compound of choloidic acid with allantoine and water: Choloid. acid. Allant. Water. Choleic acid. Or as a compound of cholic acid, urea, and water: Cholic acid. Urea. Water. Choleic acid. C 74 H 60 O 18 7 f-C 2 N 2 H 4 O 2 +H 2 O 2 =C 76 N 2 H 66 O 22 46. If, in point of fact, as can hardly be doubted, the elements of such substances as starch, sugar, &c., take part in the produc- tion of bile in the organism of the herbivora, there is nothing opposed to such a view in the composition of the chief constituents of bile, as far as our knowledge at present extends. If starch be the chief agent in this pro- cess, it can happen in no other way but this that, as when it passes into fat, a cer- tain quantity of oxygen is separated from the elements of the' starch, which, for the .same amount of carbon, (for 72 atoms,) con- tains five times as much oxygen as choloidic dcid. Without the separation of oxygen from the elements of starch, it is impossible to conceive its conversion into bile; and this separation being admitted, its conversion into a compound intermediate in composition between starch and fat offers no difficulty. 47. Not to render these considerations a mere idle play with formula?, and not to lose sight of our chief object, we observe, therefore, that the consideration of the quantitative proportion of the bile secreted in the herbivora leads to the following con- clusions : The chief constituents of the bile of the herbivora contain nitrogen, and this nitrogen is derived from compounds of proteine. The bile of this class of animals contains more carbon than corresponds to the quan- tity of nitrogenized food taken, or to the por- tion of tissue that has undergone metamor- phosis in the vital process. A part of this carbon must, therefore, be derived from the non-nitrogenized parts of the food (starch, sugar, &c.;) and in order to be converted into a nitrogenized consti- tuent of bile, a part of the elements of these bodies must necessarily have combined with a nitrogenized compound derived from a compound of proteine. In reference to this conclusion, it is quite indifferent whether that compound of pro- teine be derived from the food or from the tissues of the body. 48. It has very lately been stated by A. Ure, that benzoic acid, when administered internally, appears in the urine in the form of hippuric acid. Should this observation be confirmed,* it will acquire great physiological significance, since it would plainly prove that the act of transformation of the tissues in the animal body, under the influence of certain matters taken in the food, assumes a new form with respect to the products which are its result; for hippuric acid contains the elements of lactate of urea, with the addition of those of benzoic acid : 1 at. urea . . C 2 N 2 H 4 O ? - 1 at. lactic acid . . C 6 H 4 O 4 2 at. benzoic acid C 28 H 10 O 6 ' 3 1 2 at. chrystallized hippuric = 2(C 18 NH 9 O 6 ) 49. If we consider the act of transforma- tion of the tissues in the herbivora as we have done in the carnivora, then the blood of the former must yield, as the last products of the metamorphosis, from all the organs taken together, choleic acid, uric acid, and ammonia (see p. 44 ;) and if we ascribe to the uric acid an action similar to that of the t>enzoic acid in Ure's observation such, namely, that the further transformation, owing to the presence of this acid, assumes another form, the elements of the uric acid i)eing incorporated in the final products it will appear, for example, that 2 at. of pro- * The analysis of the crystals deposited from he urine on the addition of muriatic acid has not )een performed. Besides, the statement of A* Ure, that hippuric acid, dissolved in nitric acid, is reddened by ammonia, is erroneous, and shows hat the crystals he obtained must have contained aric acid. SECRETIONS AND EXCRETIONS. 49 teme, with the addition of the elements of give rise to the production of hippuric acid 3 at. of uric acid and 2 at. of oxygen, might and urea. 2 at. proteine, 2 (C^H^O 14 ) = 3 at. uric acid, 3 (C 10 N 4 H 4 O 6 ) 2 at. oxygen = O 2 The sum is . . . = C 126 J\ 24 H M O 48 = 6 at. hippuric acid, 6 (C 18 N H 8 O 5 ) = C 108 N 6 H 48 O 30 9 at- urea . . . 9 (C 2 N 2 H 4 O 2 ) The sum is 50. Finally, if we bear in mind, that, in the herbivora, the non-nitrogenized con- stituents of their food (starch, &c.) must, as we have shown, play an essential part in the formation of the bile; that to their ele- ments must of necessity be added those of a nitrogenized compound, in order to pro- duce the nitrogenized constituents of the bile, the most striking result of the combina- tions thus suggested is this, that the elements of starch added to those of hippuric acid are equal to the elements of choleic acid, phis, a certain quantity of carbonic acid : 2 at. hippuric acid, 2 (C 18 NH 8 O 5 ) = CN 2 H 16 O 10 5 at. starch . . 5 (C 12 H 10 O 10 ) = C 60 H^O 50 2 at. oxygen . . == O 2 The sum is ..... 2 at. choleic acid 2 (C^NH^O 11 ) 20 at. carbonic acid 20 (C O 2 ) C 90 O 40 The sum is 51. Now since hippuric acid may be de- rived, along with urea, from the compounds of proleine, when to the elements of the latter are added those of uric acid (see p. 49;) since, further, uric acid, choleic acid, and ammonia contain the elements of pro- teine in a proportion almost identical with that of proteine itself (see p. 44;) it is obvious that, if from 5 at. of proteiae, with the addition of oxygen and of the elements of water, there be removed the elements of choleic acid and ammonia, the remainder will represent the elements of hippuric acid and of urea ; and that if, when this separa- tion occurs, and during the further transfor- mation, the elements of starch be present and enter into the new products, we shall obtain an additional quantity of choleic acid, as well as a certain amount of carbonic acid gas. That is to say that if the elements of proleine and starch, oxygen and water being also present, undergo transformation together and mutually affect each other, we obtain, as the product of this metamorphosis, urea, choleic acid, ammonia, and carbonic acid, and besides these, no other product whatever. The elements of 5 at. proteine "| f 9 at. choleic acid 15 at. starch I 9 at. urea 12 at. water f | ^ at - ammoma 5 at. oxygen J [_60 at. carbonic acid In detail 5 at. proteine, 5 (CN 8 H*0 14 ) = C^N^H^O 70 15 at. starch, 15 (C 12 H 10 O 10 ) C 180 H 150 O 1M 12 at. water, 12 ( HO ) = H 12 O 12 5 at. oxygen = O 5 The sum is . . . . = and 9 at. choleic acid, 9 (C^NH^O 11 ) = 9 at. urea, . . . 9 (C 2 N 2 H 4 O 2 ) = C 18 N 18 H M O 18 3 at. ammonia, . 3( N H 3 ) = N 3 H 9 60 at. carbonic acid, 60(0 O 2 ) = C 60 O 120 The sum is = CNH 3 O iW The transformation of the compounds of pioteine present in the body is effected by means of the oxygen conveyed by the arte- rial blood, and if the elements of starch, rendered soluble in the stomach, "and thus carried to every part, enter into the newly formed compounds, we have the chief con- stituents of the animal secretions and ex- cretions ; carbonic acid, the excretion of the luugs, urea and carbonate of ammonia, ex- creted by the kidneys, and choleic acid, se- creted by the liver. Nothing, therefore, in the chemical com- position of those matters which may be supposed to take a share in these metamor- phoses, is opposed to the supposition that a part of the carbon of the non azotized food enters into the composition of the bile. 52. Fat, in the animal body, disappears when the supply of oxygen is abundant. ANIMAL CHEMISTRY. When that supply is deficient, choleic acid may be converted into hippuric acid, litho- felMc acid, (37) and water. Lithofellic 2 at. choleic acid C 76 N 2 H 66 O 22 10 at. oxygen . . O 10 acid is known to be the chief constituent of the bezoar stones, which occur in certain herbivorous animals : hip. acid C^NWO 10 lith. acid C 40 H 36 8 water . . H"Q l\s UV4 lu'* V Wl V. f 2 at. = <^ 1 at. \_14at. 53. For the production of bile in the animal body a certain quantity of soda is, in all circumstances, necessary; without the presence of a compound of sodium no bile can be formed. In the absence of soda, the metamorposis of the tissues composed of proteine can yield only fat and urea. If we suppose fat to be composed according to the empirical formula C U H 10 O, then, by the addition of oxygen and the elements of water, to the elements of proteine, we have the elements of fat, urea, and carbonic acid Proteine. Water. Oxygen. 2 (C 48 N 6 H 36 O 14 ) -f 12 HO 4- 14 O = C^N^H^O 54 = 6 at. urea . . . = C^N^H^O 12 Fat == C 66 H 60 6 18 at. carbonic acid = C 18 O 36 The composition of all fats lies between the empirical formula? C n H 10 O and C 12 H 10 O. If we adopt the latter, then the elements of 2 at. proteine, with the addition of 2 at. oxygen and 12 at. water, will yield 6 at. urea, fat (C^H^O 6 ), and 12 at. carbonic acid. It is worthy of observation, in reference to the production of fat, that the absence of common salt (a compound of sodium which furnishes soda to the animal organism) is favorable to the formation of fat; that the fattening of an animal is rendered impossi- ble, when we add to its food an excess of salt, although short of the quantity required to produce a purgative effect. 54. As a kind of general view of the metamorphoses of the nitrogenized animal secretions, attention may here be very pro- perly directed to the fact, that the nitrogen- ized products of the transformation of the bile are identical in ultimate composition with the constituents of the urine, if to the laxter be added a certain proportion of the elements of water. 1 at. uric acid C 10 N 4 H 4 O 6 } 1 at. urea ...C 2 N 2 H 4 O 2 ^ 22 at. water . . H 22 O 22 3 _ 3 at. taurine ~ 3 at. ammonia N 3 H 9 C 12 J\ 6 H 30 O 30 1 at. allantoine C 4 N 2 H 3 O 3 ? 1 at. water . . H 7 O 7 $ 1 at. taurine C 4 N H 7 O 10 \ at. ammonia N H 3 1 a 14 a 2 at. oxygen 55. In reference to the metamorphoses of uric acid of the products of the transforma- tion of the bile, it is not less significant, and worthy of remark, that the addition of oxy- gen and the elements of water to the ele- ments of uric acid may yield either taurine and urea, or taurine, carbonic acid, and am- monia. at ' taurine l at - urea H4 C io N 4 H i8 O 22^ c 10 ; 1 T2 at. taurine C 8 N 2 H 14 20 f O * W ill 1840. November, in th period from the INN J CO OOOCO JO JO O CO jgO t^ t^O COCO CO CO CO NOO(MO OOO . " ;2COCO COCO COCO Ci CO O* IO i S 2 oS 05 N ** O "fll 01 t 5 ri 4 g 21 O s O " .a-fl ^^^ s :: e ns^.Sffi d ^ c o fe 2 10 C3 4_> ^ O! 03 ifrPi d H I d *& .t; i o> 3 APPENDIX. ANALYTICAL EVIDENCE. 85 The faeces of a soldier weigh 5*5 oz., and contain, in the fresh state, 11 per cent, of carbon. For 86 kreutzer (about 2s. 5d. sterling,) there may be bought, on an average, 172 Ibs. of vegetables, such as cabbages, greens, turnips, &,c.; 25 maas of sour krout weigh 100 Ibs. ; and for 48$ kreutzer (Is. 55. sterling,) there are brought, on an average, 24i Ibs. of onions, leeks, celery, &c.* 855 men consumed Of green vegetables 2,802 oz. Of sour krout 1,600 Of onions, &c 388 .01 all 4,790 And one man 5-6 oz. For this reason, the carbon of the last mentioned articles of food has been assumed as equal to that of the faeces and urine. Sausages, brandy, beer, in short, the small quantity of food taken irregularly in the alehouse, has not been included in the calculation. The daily allowance of bread, being uniformly 2 Ibs. per man, with the exceptions formerly mentioned, has not been inserted in the table, which includes only those matters of which, from the daily allowance being variable, an average was required. The small quantity of bread in the table is that given in the soup, which is over and above the daily supply. NOTE (4.) See next page. NOTE (5,) p. 15. TEMPERATURE OP THE BLOOD AND FREQUENCY OF THE PULSE. According to Prevost and Dumas. . The .frequency The mean temperature ia of the pulse of the respiration F. in the minute. in the minute. In the Pigeon . . 107-6 . . 136 . .34 Common Fowl . 1067 140 . . 30 Duck . . . 108-5 . . 170 . .21 Raven . . . 108-5 . . 110 . . 21 Lark . . . 117-2 . . 200 . .22 Simia Callitriche . 95-9 . 90 . . 30 Guinea Pig . . 100-4 . . 140 . .36 Dog ... 99-3 . . 90 . . 28 Cat .... 101-3 . . 100 . .24 Goat . . . 102-5 . 84 . . 24 Hare .... 100-4 . . 120 . .36 Horse . . . 98-2 . 56 . . 16 Man 98-6 72 18 Man(Liebig) . . 977 . . 65 . .17 Woman (Liebig) . 982 . 60 . . 15 The temperature of a child is 102-2. The temperature of the human body, in the mouth or in the rectum, for example, is from 97-7 to 98-6. That of the blood (Majendie) is from 100'6 to 101-6. As a mean temperature, 99*5 has been adopted in this work, page 15. NOTE (6,) p. 20. The prisoners in the house of arrest of Giessen receive daily 1$ Ib. of bread (24 oz.,) which contain 7 oz. of carbon. They receive, besides, 1 Ib. of soup daily, and on each alternate day, 1 Ib. of potatoes. 1^ Ib. of bread contains .... 7'25 oz. of carbon. 1 Ib. of soup contains 075 ditto. Ib. of potatoes contains .... 1*00 ditto. Total 9-00 ditto.f * In the original table, the quantities of these vegetables are entered according to their value in Kreutzers, but they are here calculated by weight from the above data, as this appeared better adapted for comparison in this country than the prices would have been. ED. t At page 36 the carbon contained in the daily food of these prisoners is calculated at 8^ oz., and ANIMAL CHEMISTRY. a ^- 0) g* I 10 10 p O CO IO G^CO 10 o ' SS Potatoes After G Water i >, Q bfl l co 10 C^CO o oc s ^ 03 EH 00 II , >, fl - O bo &' S 050 oo co CO b* IO si CS o 'C M frl*i 10 8 APPENDIX. ANALYTICAL EVIDENCE. 87 NOTE (7,) p. 21. COMPOSITION OF THE FIBRINE AND ALBUMEN OF BLOOD, a. Albumen from Serum of Blood. Fibrine. Scherer.* Scherer. Mulder. 53-850 6-983 15-673 n. 55-461 7-201 15673 in. 56-097 6-880 15-681 53-671 6-878 15-763 n. 54-454 7-069 15-762 in. 54-56 6-90 15-72 23-494 21-655 22-342 23-688 22-715 22-82 Carbon Hydrogen Nitrogen Oxygen Sulphur Phosphorus a Annalen der Chem. und Pharm., XXVIIL, 74, and XL., 33, 36. For additional analyses of animal fibrine and albumen, see Note (27,) which also contains analyses of the various animal tissues. NOTE (8,) p. 22. COMPOSITION OF VEGETABLE FIBRINE, VEGETABLE ALBUMEN, VEGETABLE CASEINE, AND VEGETABLE GLUTEN. VEGETABLE FIBRINE. GLUTEN, As obtained from wheat flour. Sherer*ct. A Jones.*6 IV. 53-83 7-02 15-58 Marcet.c 557 14-5 7-8 Boussing II. 53-5 150 7-0 53-064 7-132 15-359 II. 54-603 7-302 15-809 in. 54-617 7-491 15-809 24-445 22-285 22-083 23-56 22-0 24-5 Carbon Hydrogen Nitrogen Oxygen Sulphur Phosphorus I Ann. der Chem. und Pharm., XL., 7. 6 Ibid., XL., 65. c L. Gmelin's Theor. Chemie, II., 1092. VEGETABLE ALBUMEN, O. Wheat. Gluten. Almonds. Jones.* Varrentiapp & Will.* Jones.* 55-01 54-85 57-03 7-23 6-98 7-53 15-91 15-88 13-48 Carbon . Hydrogen Nitrogen Oxygen Sulphur Phosphorus From Rye. Jones.* 54-74 7-77 15-85 21-64 21-84 22-39 21-96 Carbon Hydrogen Nitrogen . Oxygen, &c. . Varrentrapp and Will:* 15-70 Boussingault. . 52.7 6-9 . 18-4 22-0 a Ann. der Chem. und Pharm., XL, 66, and XXXIX., 291. VEGETABLE CASEINE.a Sulphate of Caseine and Potash. Scherer.* Jones.* Varrentrapp and Will. Carbon Hydrogen Nitrogen Oxygen, &c. 54-138 7-156 15-672 23-034 55-05 7-59 15-89 21-47 51-24 6-77 13-23 a Ann. der Chem. und Pharm., XXXIX., 291, and XL., 8 and 67. VEGETABLE GLUTEN. Jones.*a Boussingault. Carbon . . . 55-22 1?2 5?3 Hydrogen . . . 7-42 7-5 6'5 Nitrogen . . . 15-98 13'9 18-9 Oxygen, &c. . 21-38 24-4 22-3 a Ann. der Chem. und Pharm., XL., 66. The pure gluten, analyzed by Jones, was that portion of the raw gluten from wheat flour which is soluble in hot alcohol. The insoluble portion is vegetable fibrine, the analysis of which has been already given. *'ie appendix in the original makes the number also 8'5, apparently by an error in adding up the above numbers, which yield the sum of 9 oz. Possibly there may be an error in excess in the proportion of carbon calculated for thr soup, which, in that case, ought to be 0'25 oz. EDITOR. ANIMAL CHEMISTRY. NOTE (9,) p. 24. COMPOSITION OF ANIMAL CASEINE.Cf Scherer. Carbon Hydrogen Nitrogen , Oxygen ? From fresh From sour From milk by milk. milk. acetic acid. Albuminous sub- stance in milk .6 54-507 6-913 15-670 I. II. III. IV. 54-825 54-721 54-665 54-580 7-153 7-239 7-465 7-352 15-628 15-724 15-724 15-696 22-394 22-316 22-146 22-372 22-910 Sulphur a Ann. der Chem. und Pharm., XL., 40 et seq. 6 This substance, called, in German, zieger, is contained in the whey of milk after coagulation by an acid. It is coagulated by heat, and very much resembles albumen. Mulder.a Carbon . . ." . . . 54-96 Hydrogen 7-15 Nitrogen 15-89 Oxygen 2173 Sulphur 0-36 a For the analysis of vegetable caseine, see the preceding note. NOTE (10,) p. 27. AMOUNT OF MATTER SOLUBLE IN ALCOHOL IN THE SOLID EXCREMENTS OF THE HORSE AND COW. (WILL.*) 18-3 grammes of dried horse-dung lost, by the action of alcohol, 0-995 gramme. The residue, wnen dry, had the appearance of saw-dust, after it had been deprived, by boiling, of all soluble matter. 14-98 grammes of dry cow-dung lost, by the same treatment, 0-625 gramme. NOTE (11,) p. 28. COMPOSITION OF STARCH, d Strecker.* Carbon Hydrogen Oxygen , Carbon . Hydrogen Oxygen Carbon . Hydrogen Oxygen . Calculated From From From From C19H10O10. Peas. Lentils. Beans. Buckwheat. 44.91 44-33 44-46 44-16 44-23 . 6-11 6-57 6-54 6-69 6-40 48-98 49-09 49-00 49-15 49-37 Strecker.* F rom maize. From horse-chestnuts. From wheat. From rye. 44-27 44-44 44-26 44-16 6-67 6-47 6-70 6-64 49-06 49-08 49-04 49-20 Strecker.* From rice. 44-69 6-36 48-95 From potatoes. From dahlia-roots. 44-13 6-56 49-31 From unripe apples. 44-10 6-57 49-33 From unripe pears. 44-14 6-75 49-11 From arrow-root. From yams.a Berzelius. Gay Lussac & Thenard. Prout. Ortigosa, Carbon . 44-250 43-55 44-40 44-2 Hydrogen . 6-674 6-77 6-18 6-5 Oxygen . 49-076 49-68 49-42 49-3 a The starch employed for the analyses, made by Strecker and Ortigosa, was prepared from the chemical laboratory at Giessen, from the respective seeds, bulbs, and fruits. NOTE (12,) p. 28. COMPOSITION OF GRAPE SUGAR. (STARCH SUGAR.) From gra .pes.a From starch.6 From honey.c Calculated. DeSaussure. Prout. " C12H14O14. Carbon . 36-71 37-29 36-36 36-80 Hydrogen . 6-78 6-84 7-09 7-01 Oxygen . 56-51 55-87 56-55 56-19 a A.UII. de Chiinie, XL, 331. 6 Ann. of Philosophy, VI., 426. c Philosoph. Trans. 1827, 373. APPENDIX. ANALYTICAL EVIDENCE. NOTE (13,) p. 29. 89 Gay Lussac and Theuard. Prout. COMPOSITION OF SUGAR OF MILK. Calculated Brunn. Berzelius. Liebig.* C12H12012. Carbon . 38-825 40-00 40-437 39-474 40-00 40-46 Hydrogen 7-341 6-66 6711 7-167 6-73 6-61 Oxygen. 53-834 53-34 52-852 53-359 53.27 52-93 Gay Lussac and Thenard. NOTE (14,) p. 29. COMPOSITION OF GUM. Carbon Hydrogen Oxygen 42-23 6-93 50-84 Goebel. 42-2 6-6 51-2 Berzelius. 42-682 6-374 50-944 NOTE.(15,) p. 29. ANALYSIS OF OATS. (Boussingault.) a. 100 parts of oats contain of dry matter . Ditto water Calculated. C12H11O11. 42-58 6-37 51-05 82-9 17-1 100-0 100 parts of oats dried at 212 ==117-7 parts dried at the ordinary temperature, contain Carbon . . 50-7 Hydrogen . . 64 Oxygen . . 36-7 Nitrogen . . 2-2 Ashes . . 4-0 Water 100-0 17-7 Oats dried in the air 117-7 contain, in 100 parts, 1-867 of nitrogen. a Ann. de Chimie et de Phys,, LXXI., 130. ANALYSIS OF HAT. 100 parts of hay dried in the air contain 86 of dry matter, 14 of water. 100 100 parts of hay dried at 212= 116*2 parts dried in air, contain Carbon L . . 45-8 Hydrogen . . 5-0 Oxygen . . 38-7 Nitrogen . . 1-5 Ashes 9-0 100-0 16-2 water, 116-2 hay dried in the air. 100-0 of hay dried at the ordinary temperature contain 1-29 of nitrogen. 240 oz. of such hay-=15 Ibs. contain , . 3-095 oz. of nitrogen. 72 oz. of oats 4 Ibs. contain . 1-34 ditto Total 4-435 ditto NOTE (16,) a, p. 30. AMOUNT OF CARBON IN FLESH AND IN STARCH. 100 parts of starch contain 44 of carbon ; therefore, 64 oz. (4 Ibs.) contain 28-16 oz. of carbon. 100 parts of fresh meat contain 13*6 of carbon (see Note III.;) hence 240 oz. (15 Ibs.) contain 32-64 oz. of carbon.* * By an error in calculation in the original, the amount of carbon in 15 Ibs. of meat is stated to be 27'64 oz. It follows, that the carbon of 4 Ibs. of starch is not equal, as stated in the text, to that of 15 Ibs- of flesh, but to that of 13 Ibs. This difference, however, is not sufficient to affect the argu- ment at p. 32. EDITOR. 90 ANIMAL CHEMISTRY. NOTE (16,) b, p. 32. COMPOSITION OF Hog's Lard. Mutton fat. Human fat, Chevreul. a Carbon . 79-098 78-996 Hydrogen . . 11-146 11-700 Oxygen . . . 9756. 9-304 a Recherches Chim., sur les Corps Gras. Paris. 79-000 11-416 9-584 1823. NOTE (17,) p. 32 COMPOSITION OF CANE SUGAB. According to Berzeliu, Prout. W.Crum. Liebig. f Carbon 42-225 42-86 42-14 42-301 42-47 42-58 Hydrogen 6-600 6-35 6-42 6-384 6-90 6-37 Oxygen 51-175 50-79 51-44 51-315 50-63 51-05 For the composition of gum and of starch, see Notes (14) and (11) NOTE (18,) p. 32. COMPOSITION OF CHOLESTERINE. According to Chevreul. a Couerbe. 6 Marchand. Calculated C36H32O. 85-095 84-895 84-90 84-641 11-880 12-099 12-00 12-282 3-025 3-006 3-10 3-077 Carbon . Hydrogen Oxygen a Recherches sur les Corps Gras, p. 185. 6 Ann. de Ch. et de Phys. LVI., p. 164. NOTE (19,) p. 33. THE PRODUCTION OF WAX FROM SUGAR.* As soon as the bees have filled their stomach, or what is called the honey bladder, with honey, and cannot deposit it for want of cells, the honey passes gradually in large quan- tity into the intestinal canal, where it is digested. The greater part is expelled as excre ment ; the rest enters the fluids of the bee. In consequence of this great flow of juices a fatty substance is produced, which oozes out on the eight spots formerly mentioned, which occur on the four lower scales of the abdominal rings, and soon hardens into laminae of wax. On the other hand, when the bees can deposit their honey, only so much enters the intestinal canal as is necessary for their support. The honey bladder need not be filled with honey longer than forty hours in order to bring to maturity, on the eight spots, eight laminae of wax, so that the latter fall off. I made the experiment of giving to bees, which I had enclosed in a box with their queen about the end of September, dis- solved sugar candy instead of honey. Out of this food laminae of wax were formed; but these would not separate and fall off readily, so that the mass, which continued to ooze out, remained, in most of the bees, hanging to the upper lamina : and the laminae of wax became as thick as four under ordinary circumstances. The abdominal scales of the bees were, by means of the wax, distinctly raised, so that the waxen lamina? projected between them. On examination, I found that these thick laminae, which under the microscope exhibited several lamellae, had a sloping surface downwards near the head, and upwards in the vicinity of the tail. The first waxen laminae, therefore, must have been pushed downwards by the second, because, where the abdominal scales are attached to the skin, there is no space for two laminae, the second by the third, and thus the inclined surfaces on the sides of the thick laminae had been produced. I saw distinctly from this, that the first formed laminae are detached by those which followed. The sugar had been converted into wax bythe bees, but it would seem that there was some imperfection in the process, as the laminae did not fall off, but adhered to the succeeding ones. In order to produce wax in the manner described, the bees require no pollen, but only honey. I have placed, even in October, bees in an empty hive, and fed them with honey ; they soon formed comb, although the weather was such that they could not leave the hive. I cannot, therefore, believe that pollen furnishes food for the bees, but I think they only swallow it, in order, by mixing it with honey and water, to prepare the liquid food for 'the grubs. Besides, bees often starve in April, when their stock of honey is con- sumed, and when they can obtain in the fields abundance of pollen, but no honey. * From F. W. Gundlach's Natural History of Bees, p. 115. Cassel, 1842 We are acquainted with no more beautiful or convincing proof of the formation of fatty matter from sugar than the fol- lowing process of the manufacture of wax by the bees, as taken from observation. APPENDIXANALYTICAL EVIDENCE. 91 When pressed by hunger they tear the nymphae out of the cells, and gnaw them in order to support life by the sweet juice which they contain. But, if in this condition they are not artificially fed, or if the fields do not soon yield their proper food, they die in the course of a few days. Now, if the pollen were really nourishment for bees, they ought to be able to support life on it, mixed with water. Bees never build honeycomb unless they have a queen, or are provided with young out of which they can educate a queen. But if bees be shut up in a hive without a queen, and fed with honey, we can perceive in forty-eight hours that they have laminse of wax on their scales, and that some have even separated. The building of cells is therefore voluntary, and dependent on certain conditions, but the oozing out of wax is involuntary. One might suppose that a large proportion of these laminae must be lost, since the bees may allow them to fall off, out of the hive as well as in it; but the Creator has wisely provided against such a loss. If we give to bees engaged in building cells honey in a flat dish, and cover the dish with perforated paper, that the bees may not be en- tangled in the honey, we shall find, after a day, that the honey has disappeared, and that a large number of laminae are lying on the paper. It would appear as if the bees, which nave carried off the honey, had let fall the scales ; but it is not so. For, if above the paper we lay two small rods, and on these a board, overhanging the dish on every side, so that the bees can creep under the board and obtain the honey, we shall find next day the honey gone, but no laminae on the paper ; while laminae will be found in abundance on the board above. The bees, therefore, which go for and bring the honey, do not let fall the laminae of wax, but only those bees which remain hanging to the top of the hive. Repeated experiments of this kind have convinced me that the bees, as soon as their laminae of wax are mature, return to the hive and remain at rest, just as caterpillars do, when about to change. In a swarm that is actively employed in building we may see thousands of bees hanging idly at the top of the hive. These are all bees whose laminae of wax are about to separate. When they have fallen off, the activity of the bee revives, and its place is occupied for tfye same purpose by another. (From page 28 of the same work.) In order to ascertain how much honey bees re- quire to form wax, and how often, in a swarm engaged in building, the laminae attain maturity and fall off, I made the following experiment, which appears to me not unin- teresting. On the 29th of August, 1841, at a time when the bees could obtain in this district no farther supply of honey from the fields, I emptied a small hive, placed the bees in a small wooden hive, having first selected the queen bee, and shut her up in a box, fur- nished with wires, which I placed in the only door of the hive, so that no embryoes could enter the cells. I then placed the hive in a window, that I might be able to watch it. At 6 P. M. I gave the bees 6 oz. of honey run from the closed cells, which had thus the exact consistence of freshly made honey. This had disappeared next morning. In the evening of the 30th I gave the bees 6 oz. more, which, in like manner, was removed by the next morning ; but already some laminae of wax were seen lying on the paper with which the honey was covered. On the 31st August and the 1st September the bees had in the evening 10 oz., and on the 3d of September in- the evening 1 7 oz. ; in all, there- fore, 1 Ib. 13 oz. of honey, which had run cold out of cells which the bees had already closed. On the 5th of September I stupified the bees, by means of puff-ball and counted them. Their number was 2,765, and they weighed 10 oz. I next weighed the hive, the combs of which were well filled with honey, but the cells not yet closed ; noted the weight, and then allowed the honey to be carried off by a strong swarm of bees. This was completely effected in a few hours. I now weighed it a second time, and found it 12 oz. lighter; consequently the bees still had in the hive 12 oz.of the 29 oz. of honey given to them. I next extracted the combs, and found that their weight was f- of an ounce. I then placed the bees in another box, provided with empty combs, and fed them with the same honey as before. In the first few days they lost daily rather more than 1 oz. in weight, and afterwards half an ounce daily, which was owing to the circumstance, that from the digestion of so much honey, their intestinal canal was loaded with excre- ments; for 1,170 bees, in autumn, when they have been but a short time confined to the hive, weigh 4 oz. ; consequently 2,765 bees should weigh 9 oz. But they actually weighed 10 oz., and therefore had within them 1 oz. of excrement, for their honey bladders were empty. During the night the weight of the box did not diminish at all, because the small quantity of honey the bees had deposited in the cells, having already the proper consistence, could not lose weight by evaporation, and because the bees could not then get rid of their excrements. For this reason, the loss of weight occurred always during the day. If, then, the bees, in seven days, required 3 oz. of honey to support and nourish their bodies, they must have consumed 13$ oz. of honey in forming of an ounce of wax; and consequently, to form 1 Ib. of wax, 20 Ibs. of honey are required. This is the reason why the strongest swarms in the best honey seasons, when other hives, that have no occasion to build, often gain in one day 3 or 4 Ibs. in weight, hardly become heavier, although their activity is boundless. All that they gain is expended in making wax. 92 ANIMAL CHEMISTRY. This is a hint for those who keep bees, to limit the building of comb. Cnauf has already recommended this, although he was not acquainted with the true relations of the subject. From 1 oz. of wax, bees can build cells enough to contain 1 Ib. of honey. 100 laminae of wax weigh 0*024 gramme (rather more than of a grain,) consequently, 1 kilogramme (= 15,360 grains) will contain 4,166,666 laminse. Hence, of an ounce will contain 81,367 laminae. Now this quantity was produced by 2,765 bees in six days; so that the bee requires for the formation of its 8 laminae (one crop) about thirty-eight hours, which agrees very well with my observations. The laminae, when formed, are as white as bleached wax. The cells also, at first, are quite white, but they are coloured yellow by the honey, and still more by the pollen. When the cold weather comes on, the bees retire to the hive under the honey, and live on the stock they have accumulated. P. 54. Many believe that bees are hybernating animals ; but the opinion is quite erro- neous. They are lively throughout the winter j and the hive is always warm in conse- quence of the heat which they generate. The more numerous the bees in a hive, the more heat is developed ; and hence strong hives can resist the most intense cold. It once happened that I forgot to remove from the door, which was unusually large, of a hive in winter, a perforated plate of tinned iron, which I had fastened over the opening to diminish the heat in July; and yet this hive came well through the winter, although the cold was very severe, having been for several days so low as 0. But I had added to this hive the bees of two other hives ! When the cold is very intense, the bees begin to hum. By this means respiration is accelerated and the developement of heat increased. If, in summer, bees without a queen are shut up in a glass box, they become uneasy and begin to hum. So much heat is by this means developed, that the plates of glass become quite, hot. If the door be not opened in this case, or if air be not admitted, and if the glass be not cooled by the aid of water, the bees are soon suffocated. COMPOSITION OF BEES' WAX. Gray Lussac Calculated and Thenard.a De Saussure.6 Oppermann.c Ettling.d Hess.e C20H20O. Carbon . 81784 81-607 81-291 81-15 81-52 81-38 Hydrogen . 12-672 13.859 14-073 13-75 13-23 13-28 Oxygen . 5-544 4-534 4-636 5-09 5-25 5-34 a Traite de Chimie, par M. Thenard, 6me. Ed. TV., 477. 6 Ann. de Ch. et de Phys. XIII., 310. c Ibid. XLIX., 224. d Annal. der Pharm., II., 267. e Ibid. XXVII., 6. NOTE (21) a, p. 36. COMPOSITION OF HYDRATED CYANTJRIC ACID, OR HYDRATED CYANIC ACID, AND OF CYAME- LIDE, IN 100 PARTS, ACCORDING TO THE ANALYSIS OF W5HLER AND LIEBIG.*a Cyanuric acid, cyanic acid, cyamelide. Carbon 28-19 ' Hydrogen 2-30 Nitrogen 32-63 Oxygen 36-87 a Poggendorff's Annalen, XX., 375 et seq. NOTE (21) b. p. 36. COMPOSITION OF ALDEHYDE, METALDEHYDE, AND ELALDEHYDE.O Aldehyde. Metaldehydc. Elaldehyde. Calculated Liebig.* Fehling.* C4H4O3. Carbon . 55-024 ' 54-511 54-620 54-467' 55-024 Hydrogen . 8-983 9-054 9-248 9-075 8-983 Oxygen . 35-993 36-435 36-132 36-458 35-993 a Ann. der Pharm., XIV., 142, und XXVIL, 319. NOTE (22,) p. 37. COMPOSITION OF PROTEINE. From the crystalline lens. From albumen. Fro fibrine. Scherer.a Carbon . . 55-300 55-100 54-848 Hydrogen . . 6-940 7-055 6-959 Nitrogen . 16-216 15-966 15-847 Oxygen . . 21-544 21-819 22-346 a Ann. der Chem. und Pharm., XL., 43. APPENDIX. ANALYTICAL EVIDENCE. Schercr.a 93 Carbon . Hydrogen Nitrogen Oxygen Carbon . Hydrogen Nitrogen Oxygen From hair. From horn. C48H36N6O14. 54746 7-129 1727 22-398 55-150 7-197 15-727 21-926 55-408 7-238 15-593 21-761 54-291 7-082 15-593 23-034 55-742 6-827 16-143 21-228 a Ann. der Chem. und Pharm., XL., 43. From vegetable albumen. From fibrine. From albumen. From cheese. Mulder. a 54-99 6-87 15-66 22-48 55-44 8-95 16-05 21-56 55-30 6-94 16-02 21-74 55-159 7-176 15-857 21-808 a Ann. de Pharm., XXVIII., 75. NOTE (23,) p. 37. COMPOSITION OF THE ALBUMEN OF THE YOLK AND OF THE WHITE OF THE From the yolk. Jones.* ir. 53-45 7-66 13-34 Carbon . . 53-72 Hydrogen . . 7-55 Nitrogen . . 13-60 Oxygen ") Sulphur S. . 25-13 25-55 Phosphorus j a Ann. der Chem. und Pharm. XL., 36, ibid. 67. From the white. Scherer.* 55-000 7-073 15-920 22-007 NOTE (24,) p. 38. COMPOSITION OF LACTIC ACID. C6H505. Carbon . .... 44-90 Hydrogen 6-11 Oxygen 48-99 NOTE (25,) p. 39. GAS FROM THE ABDOMEN OF COWS AFTER EATING CLOVER TO EXCESS, OBTAWXD BY PUNCTURE. a Examined by Lameyran and Fremy. b By Vogel. c By Pfluge. Air. Carbonic acid. Inflammable gas. Sulphuretted hydrogen. a 5 5 15 80 Vol. in 100 Vol. b 25 27 48 c _ 60 40 c 20 80 NOTE (26,) p. 40. MAGENDIE FOUND IN THE STOMACH AND INTESTINES OF EXECUTED CRIMINALS : a In the case of an individual who had taken food in moderation one hour previous to death ; i, in the case of one who had done so two hours previously; and c, in the case of a third, who had done so four hours previous to execution. 100 Volumes of the gas contained: Oxygen. Nitrogen. Carbonic acid. Inflammable gas. rFrom the stomach 11-00 Vol. 71-45 14-00 3-55 a< small intestines 00-00 20-03 24-39 55-53 large intestines 00-00 51-03 43-50 5-47 rFrom the stomach 00-00 00-00 00-00 00-00 b< small intestines 00-00 8-85 40-00 51-15 (. large intestines 00-00 18-40 70-00 11-60 TFrom the stomach 00-00 OO'OO 00-00 00-00 C J small intestines 00-00 66*60 25-00 8-40 ( large intestines 00-00 45'96 42-86 IMS 94 ANIMAL CHEMISTRY. NOTE (27,) referred to in NOTE (7,) p. 21. COMPOSITION OP ANIMAL ALBUMEN AND FIBRINE, AND OF TIJE DIFFERENT TISSUES OF THE BODY. 1. ALBUMEN. From eggs. From yolk of egg. From the serum of blood. Scherer.*ct Carbon Hydrogen Nitrogen Oxygen Sulphur Phosphorus I. ii. in. IV. T. TI. 53-850 55-461 55-097 55-000 53-72 53-45 6-983 7-201 6-880 7-073 7.55 7-66 15-673 15-673 15-681 15-920 13-60 13-34 23-494 21-655 22-342 22-007 25-13 25-55 a Ann. der Chem. und Pharm., XL., 36. 6 Ibid. 67. Jones.* Scherer.* From albumen From From congesti ve From fluid of brain. hydrocele. abscess. From pus. of dropsy VII. VIII. IX. X. XI. XII Carbon . 55-50 54-921 54-757 54-663 54-101 54-302 Hydrogen . Nitrogen . 7-19 16-31 7-077 7-177 15-465 15-848 7-022 6-947 15-839 15-660 7-176 15-717 Oxygen Sulphur Phosphorus >. . 21-00 22-537 22-224 22-476 23-292 22-805 Mulder.o Carbon . . 54-84 7-09 Nitrogen . . 15-83 21-23 Sulphur . . . 0-68 Phosphorus 0-33 a Ann. der Pharm. XXVIIL, 74. 2. FlBRINE. Scherer. *a Carbon Hydrogen Nitrogen Oxygen Sulphur Phosphorus I. II. .III. IV. V. VI. VII. 53-671 54.454 55-002 54-967 53-571 54-686 54-844 6-878 7-069 7-216 6-867 , 6-895 6-835 7-219 15-763 15-762 15-817 15-913 15-720 15-720 16-065 23-688 22-715 21-965 22-244 23-814 22-759 21-872 a Ann. der Chem. und Pharm., XL., 33. Carbon .... 54-56 Hydrogen .... 6-90 Nitrogen . . . ' 15.72 Oxygen .... 22-13 Sulphur . . . . 0-33 Phosphorus .... 0-36 a Ann. der Chem. und Pharm., XXVIII., 74. 3. GELATINOUS TISSUES. Scherer.*o Carbon Hydrogen Nitrogen Oxygen j Isinglass. 59-557 6-903 18-790 23-750 a Tendons of the calf's foot. Tunica Calculated, sclerotica. C48H41N7^018 49-563 7-148 18-470 24.819 Ann. der Chem. 50-960 7-188 18-320 23-532 und Pharm. 50-774 7-152 18-320 23-7^4 , XL., 46. 50-995 7-075 18-723 23-207 50-207 7-001 18-170 24-622 Mulder. Carbon Hydrogen Nitrogen Oxygen 50-048 6-477 18-350 25-125 50-048 6-643 18-388 24-921 APPENDIX. ANALYTICAL EVIDENCE. 93 4. TISSUES CONTAINING CHONDRINE. Scherer.*a Carbon Hydrogen Nitrogen Oxygen Cartilages of the ribs of the calf. Calculated Cornea. C48H40N6020 49-522 50-745 7-097 6-904 14-399 14-692 28-982 27-659 49-496 7-133 41-908 28-463 50-895 6-962 14-908 27-235 Mulder. 50-607 6-578 14-437 28-378 a Ann. der Chem. und Pharm., XL., 49. 5. COMPOSITION OF THE MIDDLE MEMBRANE OF ARTERIES. Scherer.*a Carbon Hydrogen Nitrogen Oxygen 53-750 7-079 15-360 23-811 n. 53-393 6-973 15-360 24-274 Calculated C48H38N6016 53-91 6-96 15-60 23-53 a Ann. der Chem. und Pharm., XL., 51. 6. COMPOSITION OF HORNY TISSUES. Scherer.*a External skin of the sole of the foot. Hair of the beard. 51-529 6-687 17-936 23-848 Schen Hair of the head. Fair. Brown. .A. Black. 51-036 6-801 17-225 24-938 50-752 6-761 17-225 25-262 50-652 6-769 17-936 24-643 :r.*a 49-345 . 6-576 17-936 26-143 50-622 6-613 17-936 24-829 49-9315 6-631 17-936 25-498 Calculated M8H39N7017 51-718 6-860 17-469 23-953 Buffalo horn. .Nails. 51-089 6-824 16-901 25-186 Wool. C 50-653 7-029 17-710 24-608 51-990 6-717 17-284 24-009 51-162 6-597 17-284 24-957 51-620 6-754 17-284 24-342 51-540 6-779 17-284 24-397 Carbon Hydrogen Nitrogen Oxygen ? Sulphur 5 Carbon Hydrogen Nitrogen Oxygen ? Sulphur > a. Ann. der Chem. und Pharm., XL., 53. The composition of the membrane lining the interior of the shell of the egg approaches closely to that of horn. According to^Scherer, it contains Carbon Hydrogen 6-608 Nitrogen 16-761 Oxygen ^ > 25 . 958 Scherer.*a 50-674 Sulphur a Ann. der Chem. und Pharm., XL., 60. The composition of feathers is also nearly the same as that of horn. Scherer.*a Carbon Hydrogen Nitrogen Oxygen . Beard of the feather. 50-434 7-110 17-682 , 24-774 Quill of the feather. 52-427 7-213 17-893 22-467 Calculated , C48H39IV7OI6. 52-457 6-958 17-719 22-866 a Ann. der Chem. und Pharm., XL., 61. ^ The analysis here given of the beard of feathers agrees closely with that of horn, while that of the quill is more accurately represented by the attached formula, which differs from that of horn by 1 eq. of oxygen only. ^ 7. COMPOSITION OF THE PIGMENTUM NIGRUM OCULI. ""~ Scherer.*a Carbon Hydrogen Nitrogen Oxygen 58-273 5-973 13-768 21-986 58-673 5-962 13-768 21-598 57-908 5-817 13-768 22-507 a Ann. der Chem. und Pharm^ XL., 63. 96 ANIMAL CHEMISTRY. NOTE (28,) p. 44. According to the analyses of Playfair and Bceckmann, 0-452 parts of dry muscular flesh gave 0-836 of carbonic acid. 0-407 ...... 0-279 of water. 0-242 ...... 0-450 of carb. acid and 0-164 water. 0191 ...... 0-360 . . . 0-130 0-305 of dried blood gave 0-575 carbonic acid and 0-202 of water. 0-214 . . . 0-402 . . . 0-138 1-471 of dried blood, when calcined, left 0-065 of ashes=4-42 pr. cent. The dried flesh was found to contain of ashes 4-23 pr. cent. The nitrogen was found to be to the carbon as 1 to 8 in equivalents. Hence Carbon Hydrogen Nitrogen Oxygen Ashes Deducting the ashes, or inorganic matter, the composition of the organic part is, Carbon . . . 54-12 54-18 54-19 54-20 Hydrogen . . . 7-89 7-93 7-48 7-65 Nitrogen . . 15-67 15-71 15-72 15-73 Oxygen . . . 22-32 22-18 22-31 22-12 This corresponds to the formula C 48 ...... 54-62 H 39 ...... 7-24 N 8 ..... 15-81 Q 15 ...... 22-33 Flesh (beeD Ox-blood. Blood. Playfair. 51-83 Bceckmann. 51-89 Playfair. 51-95 Bceckmann. Mean of 2 analyses. 51-96 51-96 7-57 7-59 7-17 7-33 7-25 15-01 15-05 15-07 15-08 15-07 21-37 21-24 21-39 21-21 21-30 4-23 4-23 4-42 4-42 4-42 Carbon Hydrogen Nitrogen Oxygen NOTE (29,) p. 44. COMPOSITION OP CHOLEIC ACID, d Demar emarcay. . 63-707 8-821 . 3-255 24.217 Dumas. 63-5 9-3 3-3 23-9 Calculated C76H66N3O22. 63-24 8-97 3-86 23-95 a Ann. der Pharm., XXVIL, 284 and 293. NOTE (30,) p. 44. COMPOSITION OF TAURINE AND OP CHOLOIDIC ACID. 1. TAURINE. a Carbon Hydrogen Nitrogen Oxygen Demargay.* . 19-24 5-78 .11-29 63-69 Dumas. 19-26 5-66 11.19 63-89 Calculated. C4H7NO16 19-48 5-57 11-27 63'68 a Ann. der Pharm., XX VII., 287 and 292. 2. CHOLOIDIC ACID, a Demargay.* A Dumas. Carbon Hydrogen Oxygen . 73-301 73-522 73-3 . 9-511 9-577 9-7 . 17-188 16-901 17-0 a Ann. der Pharm., XXVII., 289 and 293. Calculated. C36H56O12. 74-4 9-4 16-2 In reference to the researches of Demargay on the bile I would make the following ob- servations. The matter to which I have given the name of choleic acid is the bile itself separated from the inorganic constituents (salts, soda, &c.) which it contains. By the action of subacetate of lead aided by ammonia, all the organic constituents of the bile are made to unite with oxide of lead, with which they form an insoluble, resinous precipitate. The APPENDIX. ANALYTICAL EVIDENCE. 97 substance here combined with oxide of lead contains all the carbon and nitrogen of the bile. The substance which I have named choloidic acid is that which is obtained, when the bile, purified by alcohol from the substances insoluble in that fluid, is boiled for some time with an excess of muriatic acid. It contains all the carbon and hydrogen of the bile, except those portion which have separated in the form of taurine and ammonia. The cholic acid contains the elements of bile, minus those of carbonate of ammonia. These three compounds, therefore, contain the products of the metamorphosis of the entire bile; their formulae express the amount of the elements of the constituents of the bile. No one of them exists ready formed in the bile in the shape in which we obtain it; their elements are combined in a different way from that in which they were united in the bile ; but the way in which these elements are arranged has not the slightest inflence on the determination by analysis of the relative proportions of the elements. In the formulae themselves, therefore, is involved no hypothesis ; they are simply expressions of the re- sults of analysis. It signifies nothing that the choleic or choloidic acids may be composed of several compounds united together. No matter how many such they may contain, the relative proportions of all the elements taken together is expressed by the formula which is derived from the analysis. The study of the products which are produced from the bile by the action of the at- mosphere, or of chemical re-agents, may be of importance in reference to certain patholo- gical conditions ; but except as concerns the general character of the bile, the knowledge of these products is of no value to the physiologist ; it is only a burthen which impedes his progress. It cannot be maintained of any one of the 38 or 40 substances, into which the bile has been divided or split up, that it exists ready formed in the healthy secretion ; on the contrary, we know with certainty that most of them are mere products of the action of the re-agents which are made to act on the bile. The bile contains soda ; but it is a most remarkable and singular compound of soda. When we cause that part of the bile which dissolves in alcohol (which contains nearly all the organic part) to combine with oxide of lead, thus separating the soda, and then, remove the oxide of lead, we obtain a substance, choleic acid, which, when placed in contact with soda, forms a compound similar to bile in its taste; but it is no longer bile; for bile may be mixed with organic acids, nay, even with dilute mineral acids, without becoming turbid or yielding a precipitate; while the new compound, choleate of soda, is decomposed by the feeblest acids, the whole of the choleic acid being separated. Hence, bile cannot be considered, in any sense, as choleate of soda. Further, it may be asked, in what form are the cholesterine, and stearic, and margaric acids, which are found in bile, contained in that fluid ? Cholesterine is insoluble in water, and not saponifiable by alkalies ; and if the two fatty acids just named were really present in the bile as soaps of soda, they would be instantly separated by other acids. Yet diluted acids cause no such separation of stearic and margaric acids in bile. It is possible that, in the course of new and repeated investigations, the composition of the substances obtained from bile may be found different from that which has been given in our analytical developement of this subject. But this, if it should happen, can have but little effect on our formula?; if the relative proportions of carbon and nitrogen be not changed, the differences will be confined to the proportions of oxygen and hydrogen. In that case it will be necessary for the developement of our views in formula?, only to assume that more water and oxygen, or less water and oxygen, have taken a share in the meta- morphosis, of the tissues ; but the truth of the developement of the process itself will not be by this means affected. NOTE (31,) p. 44, COMPOSITION OF CHOLIC ACID, a Dumas. Calculated C74H60018. Carbon .... 68-5 . . .68-9 Hydrogen ... 9.7 ... 9.2 Oxygen . . . .21.8 . . .21.9 a Ann. der Pharm. XXVII., 295. NOTE (32,) p. 45. COMPOSITION OF THE CHIEF CONSTITUENTS OF THE URINE OF MEN AND ANIMALS. 1. URIC ACID. Liebie.*a Mitscherlich.6 Calculated C10H4N4O6. Carbon . . 36-083 35-82 36-00 Hydrogen . . 2-441 2-38 23-6 Nitrogen . . 33-361 34-60 33.37 Oxygen . . 28.126 27-20 28-27 a Ann. der Pharm., X.. 47. 6 Poggendorff's Ann., XXXIII- , 335. 13 ANIMAL CHEMISTRY. 2. ALLOXAN. a A PRODUCT OF THE OXIDATION OF URIC ACID. Woehler and Liebig.* Calculated C8H4N2010. Carbon . Hydrogen Nitrogen Oxygen . 30-38 30-18 30-34 2-57 2-48 2-47 . 17.96 17-96 17-55 . 49-09 49-38 49.64 a Ann. der Pharm., XXVI., 260. 3. UREA. Prout. a Woehler and Liebig. b Calculated C2H4N2O2 Carbon Hydrogen Nitrogen Oxygen 19-99 20-02 20-192 6-65 6-71 6-595 . 46-65 46-73 46-782 . 26-63 26-54 26-425 a Thompson's Annals., XL, 352. 6 Poggend. Ann., XX., 375. 4. CRYSTALLIZED HIPPURIC ACID. Liebig.* a Dumas. b Mitscherlich. c Calculated C18H8NO5. Carbon JHydrogen Nitrogen Oxygen 60-742 60-5 60-63 60-76 4-959 4-9 4-98 4-92 7-816 7-7 7-90 7-82 26-483 26-9 26-49 26-50 a Ann. der Pharm., XII., 20. b Ann. de Ch. et de Phys., LVIL, 327. c Poggend. Ann., XXXIII., 335. 5. ALLANTOINE. a Woehler and Liebig.* Calculated C8H6N406 Carbon . Hydrogen Nitrogen Oxygen . 30.60 30-66 . . . 3-83 3-75 . 35-45 35-50 . . . 30-12 30-09 a Ann. der Pharm., XXVI., 215. 6. URIC ON XANTHIC OXIDE, a Woehler and Liebig.* Calculated C5H2N202. Carbon Hydrogen Nitrogen Oxygen . . . . 39-28 39-86 . . . 2-95 2-60 . . . 36-35 37-72 21-24 20-82 a Ann. der Pharm., XXVL, 344. 7. CYSTIC OXIDE, a Thaulow.* Calculated C6H6N04S4. Carbon Hydrogen Nitrogen Oxygen . Sulphur . . . . 30-01 30-31 5-10 4-94 . . ll'OO H-70 . 28-38 26-47 . 25-51 26-58 a Ann der Pharm., XXVIL, 200. APPENDIX. ANALYTICAL EVIDENCE. 99 The cystic oxide is distinguished from all the other concretions occurring in the uri- nary bladder by the sulphur it contains. It can be shown with certainty, that the sul- phur is present neither in the oxidized state, nor in combination with cyanogen ; and in regard to its origin the remark is not without interest, that four atoms of cystic oxide contain the elements of uric acid ; benzoic acid, sulphuretted hydrogen, and water j all of which are substances, the occurrence of which, in the body is beyond all doubt 1 atom uric acid . . . 1 atom benzoic acid 8 atoms sulphuret- ? ted hydrogen. . . $ C io N 4 H 4 6 C u H *0 3 H 8 S 8 H 7 O 7 4 atoms cystic oxide =4 (C 6 NH 6 O 4 S 2 ). An excellent method of detecting the presence of cystic oxide in calculi or gravel is the following : The calculus is dissolved in a strong solution of caustic potash, and to the solution is added so much of a solution of acetate of lead, that all the oxide of lead is retained in so- lution. When this mixture is boiled there is formed a black precipitate of sulphuret of lead, which gives to the liquid the aspect of ink. Abundance of ammonia is also disen- gaged ; and the alkaline fluid is found to contain, among other products, oxalic acid. NOTE (33,) p. 45. COMPOSITION OF OXALIC, OXALURIC, AND PARABANIC ACIDS. 1. OXALIC ACID (hydrated.) Calculated Gay Lussac & Thenard. Berthollet. C3 O3+HO Carbon . . 26-566 25-13 26-66 Hydrogen . . 2-745 3-09 2-22 Oxygen, . . 70-689 7178 v 7H2 2. OXALURIC ACID, a Woehler and Liebig.* Carbon Hydrogen Nitrogen Oxvgen Carbon Hydrogen Nitrogen Oxygen 27-600 3-122 21-218 48-060 27-318 3-072 21-218 48-392 a Ann. der Pharm., XXVI., 286. 3. PARABANIC ACID, a Woehler and Liebig.* A_ f 31-95 31-940 . 2-09 1-876 24-66 24-650 . 41-30 41-534 a Ann. de Pharm., XXVI., 286. Calculated C6H4N208 27-59 3-00 21-29 48-12 31-91 1-73 24-62 4174 Hence NOTE (34,) p. 45. COMPOSITION OF ROASTED FLESH. 1.) 0-307 of flesh gave 0-584 of carbonic acid and 0-206 of water. 2.) 0-255 (3.) 0-179 do. do. Carbon Hydrogen . Nitrogen Oxygen > Ashes > 0-485 0-340 Flesh of roedeer(l.) Bceckmann.* 52-60 . 7-45 15-23 . 24-72 do. do. 0-181 0-125 do. do. Flesh of Beef (2.) Flesh of real (3.) Playfair. 52-590 7-886 15-214 24.310 42-52 7-87 14-70 24.91 100 ANIMAL CHEMISTRY. NOTE (35,) p. 46. The formula C^H^N^O 40 , or C^H'WO 20 , gives, when reduced to 100 parts, C 54 50-07 H 42 . ... 6-35 N 9 19-32 O 20 24-26 Compare this with the composition of gelatine, as given in Note (27) NOTE (37,) p. 49. COMPOSITION OF DITHOFELLIC ACID.a Ettling and Will.* Wcehler." Calculated C40H3608 Carbon . . 71-19 70-80 70-23 70-83 70-83 Hydrogen . . 10-85 1078 10-95 10-60 10-48 Oxygen . . 17-96 18-42 18-82 18-57 18-69 a Annalen der Chem. und Pharm., XXXIX., 242, and XLI., 154. NOTE (38,) p. 56. COMPOSITION OF SOLAN1NE FROM THE BUDS OF GERMINATING POTATOES, Blanchet. Carbon. . . . . . 62-11 Hydrogen 8-92 Nitrogen 1-64 Oxygen . . . . . 27-33 a Ann. der Pharm., VII., 150. NOTE (39,) p. 56. COMPOSITION OF PICROTOXINE. d Francis.* Carbon 60-26 Hydrogen 5-70 Nitrogen 1-30 Oxygen 32-74 a In another analysis. M. Francis obtained 0'75 per cent, of nitrogen. The picrotoxine employed for these analyses was partly obtained from the manufactory of M. Merck, in Darmstadt, and waa partly prepared by M. Francis himself; it was perfectly white, and beautifully crystallized. Reg. nault, as is well known, found no nitrogen in this compound. NOTE (40,) p. 56. COMPOSITION OF Q.UININE. Liebig.* Calculated C20H12NO2. Carbon 75-76 74-39 Hydrogen Nitrogen . 7-52 . 8-11 7-25 8-52 Oxygen . . . . 8-62 9-64 NOTE (41,) p. 156. COMPOSITION OF MORPHIA. Carbon . Hydrogen Nitrogen . Oxygen Liebig.* . . 72-340 6-366 . 4-995 16-299 a Ann. der Pharm., Calculated Regnault. C35H20NO6 72-87 6-86 5-01 15-26 XXVI., 72-41 6-84 5-01 15-74 23. 72-28 6-74 4-80 16-18 APPENDIX. ANALYTICAL EVIDENCE. 101 NOTE (42,) p. 156. COMPOSITION OF CAFFEINE, THEINE, GUARANINE, THEOBROMINE, AND ASPARAQINE. Caffeine, a Theine. b Guaranine. c Calculated P&ffand Liebig.* Jobst. Mitrtius. C8H5N:2O2 Carbon . . 4977 50-101 49-679 49-798 Hydrogen . 5-33 5-214 5-139 5-082 Nitrogen . . 28-78 29-009 29-180 28-832 Oxygen . . 16-12 15-676 16-002 16-288 a Ann. der Pharm., I., 17. 6 Ann. der Pharm., XXV., 63. c Ann. der Pharm., XXVI., 95. Guaranine is the name given to the crystallized principle of the guarana officinalis, till it was shown to be identical with caffeine and theine, as the above analyses demonstrate^ COMPOSITION OF THEOBROMINE. O Calculated Wosfereseusky. C9H5JV3O2 Carbon . . . 47-21 46-97 46-71 46-43 Hydrogen . . 4-53 4-61 4-52 4-20 Nitrogen . . . 35-38 3538 35-38 35-85 Oxygen . . 12-88 13-04 13-39 13-51 a Ann. der Chem. und Pharm., xli., 125. COMPOSITION OF 4SPARAGINE. tt Liebig. Calculated C8H8N2O6 -f 2HO Carbon . . . U2-351 32-35 Hydrogen. . . fj-844 6-60 Nitrogen , . . 18734 ,18-73 Oxygen , . . 42-021 42-32 a Ann. der Pharm., VII., 146. ON THE CONVERSION OF BENZOIC ACID INTO HIPPURIC ACID.* BY WILHELM KELLER (From the Annalen der Chemie und Pharmacie.) So early as in the edition of Berzelius* "Lehrbuch der Chemie," published in 1831, Professor Wohler had expressed the opinion, that benzoic acid, during digestion, was probably converted into hippuric acid. This opinion was founded on an experiment which he had msde on the passage of benzoic acid into the urine. He found in the urine of a dog which had eaten half a drachm of benzoic acid with his food, an acid crys- tallizing in needle-shaped prisms, which had the general properties of benzoic acid, and which he then took for benzoic acid. (Tiedemann's Zeitschrift fur Physiologic, i. 142.) These crystals were obviously hippuric acid, as plainly appears from the statements, that they had the aspect of nitre, and, when sublimed, left a residue of carbon. But at that time hippuric acid was not yet discovered ; and it is well known that, till 1829, when these acids were first distinguished from each other by Liebig, it was uniformly con- founded with benzoic acid. The recently published statement of A. Ure, that he actually found hippuric acid in the urine of a patient who had taken benzoic acid, recalled this relation, so remarkable in a physiological point of view, and induced me to undertake the following experiments, which, at the suggestion of Professor Wohler, I made on myself. The supposed conver- sion of benzoic acid into hippuric acid has, by these experiments, been unequivocally established. I took, in the evening before bed-time, about thirty-two grains of pure benzoic acid in syrup. During the night I perspired strongly, which was probably an effect of the acid, as in general I am with great difficulty made to transpire profusely. I could perceive no other effect, even when, next day, I took the same dose three times ; indeed, even the perspiration did not again occur. The urine passed in the morning had an uncommonly strong acid reaction, even aftet it had been evaporated, and had stood for twelve hours. It deposited only the usual sedi- ment of earthy salts. But when it was mixed with muriatic acid, and allowed to stand, * To the evidence produced by A. Ure, of the conversion of benzoic acid into hippuric acid in the numan body, M. Keller has added some very decisive proofs, which I append to this work on ac- count of their physiological importance. The experiments of M. Keller were made in the laboratory of Professor Wohler, at Gb'ttingen; and they place beyond all doubt the fact that a non-azotized substance taken in the food can take a share, by means of its elements, in the act of transformation of the animal tissues, and in the formation of a secretion. This fact throws a clear light on the mode of action of the greater number of remedies ; and if the influence of caffeine on the formation of urea or uric acid should admit of being demonstrated in a similar way, we shall then possess the key to the action of quinine and of the other vegetable alkalies. J. L. 102 ANIMAL CHEMISTRY. there were formed in it long prismatic, brownish crystals, in great quantity, which, eve a in this state, could not be taken for benzoic acid. Another portion, evaporated to the consistence of syrup, formed, when mixed with muriatic acid, a magma of crystalline scales. The crystalline mass was pressed, dissolved in hot water, treated with animal charcoal, and recrystallized. By this means the acid was obtained in colourless prisms, an inch in length. Their crystals were pure hippuric acid. When heated, they melted easily; and when exposed to a still stronger heat, the mass was carbonized, with a smell of oil of bitter almonds, while benzoic acid sublimed. To remove all doubts, I determined the propor- tion of carbon in the crystals, which I found to be 6O4 per cent. Crystallized hippuric acid, according to the formula C 18 H 8 NO 6 -f- HO, contains 60-67 per cent, of carbon; crys- tallized benzoic acid, on the other hand, contains 69*10 per cent, of carbon. As long as I continued to take benzoic acid, I was able easily to obtain hippuric acid in large quantity from the urine; and since the benzoic acid seems so devoid of any inju- rious effect on the health, it would be easy in this way to supply one's self with large quantities of hippuric acid. It would only be necessary to engage a person to continue for some weeks this new species of manufacture. It was of importance to examine the urine which contained hippuric acid, in reference to the two normal chief constituents, urea and uric acid. Both were contained in it, and apparently in the same proportion as in the normal urine. The inspissated urine, after the hippuric acid had been separated by muriatic acid, yielded, on the addition of nitric acid, a large quantity of nitrate of urea. It had pre- viously deposited a powder, the solution of which in nitric acid gave, when evaporated to dryness. the well-known purple colour characteristic of uric acid. This observation is opposed to the statement of Ure ; and he is certainly too hasty in recommending ben- zoic acid as a remedy for the gouty and calculous concretions of uric acid. He seems to suppose that the uric acid has been employed in the conversion of benzoic acid into hip- puric acid; but as his observations were made on a gouty patient, it may be supposed that the urine, even without the internal use of benzoic acid, would have been found to contain no uric acid. Finally, it is clear that the hippuric acid existed in the urine in combination with a base, because it only separated after the addition of an acid. THE EN1X INDEX. A. AciJ, Acetic. Composition ; and relation to that of aldehyde, 80,81. Acid, Benzoic. Composition, and relation to that of oil of bitter almonds, 80,81. Converted into hippuric acid in the human body, 48, 101. Acid, Carbonic. Is the form in which the in- spired oxygen and the carbon of the food are given out, 14, Its formation in the body the chief source of animal heat, 15 16. Occurs combined with potash and soda, in the serum of the blood, 21. Formed by the action of oxygen on the products of the metamorphosis of the tissues, 26. Its formation may also be connected with the production of fat from starch, 32 34. Generated by putrefaction of food in the stomach of animals, 39. Also by the fermentation of bad wine in man, when it causes death by penetrating into the lungs, 39. Escapes through both skin and lungs, 39. Pro- duced, along with urea, by the oxidation of uric acid, 45. Produced with several other com- pounds, by the oxidation of blood, 45. May be formed, along with choleic acid, from hip- puric acid, starch and oxygen, 49. Also, along with choleic acid, urea, and ammonia, by the action of water and oxygen on staich and pro- teine, 49. Produced, along with fat and urea, from proteine, by the action of water and oxy- gen, in the absence of soda, 49. Combines with the compound of iron present in venous blood, and is given off when oxygen is ab- sorbed, 78. Is absorbed by the serum of blood in all states, 78. Acid, Cerebric. Its composition, 57. Its pro- perties, 58. Acid, Choleic. Represents the organic portion of the bile, 44. Its formula, 44. Its trans- formations, 42. Half its formula, added to that of urate of ammonia, is equal to the formula of blood -f- a utt l e ox ygen and water, 44. Pro- duced in the oxidation of blood, 45. Views which may be taken of its composition, 47. May be formed by the action of oxygen and water on proteine and starch, 48. Products of its oxidation, 49. Various ways in which it may be supposed to be formed in the body, 51. Its composition, 96. Cannot be said to exist ready formed in the bile, 97. Acid, Cholic. Its composition, 98. Derived from choleic acid, 44. Possible relation to choleic acid, 47. Acid, Choloidic. Its composition, 96. Derived from choleic acid, 44. Possible relation to choleic acid, 47. Possible relation to starch, 51. Possible relation to proteine, 46. Acid, Cyanic. Its formula, 81. Acid, Cyanuric. Its formula, 81. Acid, Hippuric. Its composition, 98. Appears in the urine of stall-fed animals, 31. Is de- stroyed by exercise, 31 , 45. Is probably formed in the oxidation of blood, 45. Is found in the human urine after benzoic acid has been ad- ministered, 48, 101. May be derived from pro- teine when acted on by oxygen and uric acid, 48. With starch and oxygen, it may produce choleic and carbonic acids, 48. May be derived from the oxidation of choleic acid, 49. Acid, Hydrocyanic or Prussic. Its poisonous ac- tion explained, 80. Acid, Lithofellic. Its composition, 100. Probably derived from the oxidation of choleic acid : is the chief constituent of bezoar stones, 49. Acid, Lactic. Its composition, 93. Its origin, 38. Does not exist in the healthy gastric juice, 38. Acid, Margaric. Exists in bile, 97. Acid, Muriatic. Exists in the free state in the gastric jnice, 37, 38. Is derived from common salt, 38, 52. Acid, Oxaluric. Analysis of, 99. Acid, Parabanic. Analysis of, 99. Acid, Phosphoric. Exists in the urine of the carnivora in considerable quantity, 30, 52. Its proportion very small in that of the gramini- vora, 31. Derived from the phosphorus of the tissues, 30. It is retained in the body to form bones and nervous matter, 31. Acid, Sulphuric. Exists in the urine of the car- nivora, 30, 52. Derived from the sulphur of the tissues, 30. Acid, Uric. Its composition, 98. Products of its oxidation, alloxan, carbonic acid, oxalic acid, urea, &c., 45. Is probably derived, along with choleic acid, by the action of oxygen and water on blood or muscle, 44. Disappears almost en- tirely in the system of man and of the higher animals, 24, 41. Appears as calculus, when there is a deficiency of oxygen, 44. Never occurs in phthisical cases, 45. Yields mulberry calculus when the quantity of oxygen is some- what increased, but only urea and carbonic acid with a full supply of oxygen, 45. Uric acid calculus promoted by the use of fat and of cer- tain wines, 45. Unknown on the Rhine, 45. Uric acid and urea, how related to allantoine, 46; to gelatine, 46. Forms the greater part of the urine of serpents, 24. Yields, with the elements of proteine and oxygen, hippuric acid and urea, 48. How related to taurine, 49. Calculi of it never occur in wild carnivora, but often in men who use little animal food, 47. Affinity, Chemical. Is the ultimate cause of the vital phenomena, 13. Is active only in the case of contact, and depends much on the order in which the particles are arranged, 62. Its equilibrium renders a compound liable to trans- formations, 63. In producing the vital pheno- mena, it is modified by other forces, 63. It is not alone the vital force or vitality, but is ex- erted in subordination to that force, 70. Air. Introduced into the stomach during digestion 103 104 INDEX. with the saliva, 38. Effects of its temperature and density, dry ness, &c., in respiration, 14, 15. Albumen. Animal and vegetable albumen identi- cal, 22. 23. Their composition, 87, 93. Ve- getable albumen, how obtained, 22. Is a com- pound of proteine, and in organic composition identical with fibrine and caseine, 36, 37. Exists in the yolk as well as the white of eggs, 37. Also in the serum of the blood, 21. Is the true starting point of all the animal tissues, 37. AScohol. Is hurtful to carnivorous savages, 56. Its mode of action : checks the change of mat- ter, 72. In cold climates serves as an element of respiration, 16. Aldehyde. Its composition; how related to that of acetic acid, 80, 81. Alkalies. Mineral alkalies essential both to ve- getable and animal life, 52. Vegetable alkalies all contain nitrogen, all act on the nervous sys- tem, and are all poisonous in a moderate dose, 56, 57. Theory of their action: they take a share in the transformation or production of nervous matter, for which they are adapted by their composition, 57 59. Action of caustic alkalies on bile, or choleic acid, 44. Allantoine. Is found in the urine of the foetal calf. How derived from proteine. How re- lated to uric acid and urea, 46. How related to choleic acid, 47. Its composition, 98. Allen and Pepys. Their calculation of the amount of inspired oxygen, 82. AHoxan. Formed by the oxidation of uric acid, 45. Converted by oxidation into oxalic acid and urea, oxaluric and parabanic acids, or car- bonic acid and urea, 45. How related to tau- rine, 50. Seems to act as a diuretic. Recom- mended for experiment in hepatic diseases, 45. (note.) Almonds, Bitter. Oil of. Its composition; how related to benzoic acid, 81. Ammonia. Combined with uric acid it forms the urine of serpents, birds, &c., 24. Its relation to choleic, choloidic, and cholic acids, 44. Is one of the products which may be formed by the oxidation of blood, 45; or of proteine, 48. Its relation to uric acid, urea, and taurine, 49. To allantoine and taurine, 49. To alloxan and taurine, 49. To choleic and choloidic acid and taurine, 50. To urea, water., and carbonic acid, 51. Is found in combination with acids in the urine of the carnivora, 52. Analysis. Of dry blood, 82, 96. Of dried flesh, 96. Of faeces, 83. Of black bread, 83. Of potatoes, 83. Of peas, 83. Of beans, 83. Of lentils, 83. Of fresh meat, 83. Of moist bread, 83. Of moist potatoes, 83. Of the fibrine and albumen of blood, 87, 94. Of ve- getable fibrine and albumen, vegetable caseine and gluten, 88. Of animal caseine, 88. Of starch, 88. Of grape or starch sugar, 88. Of sugar of milk, 89. Of gum, 89. Of oats, 89. Of hay, 89. Of fat, 90. Of cane-sugar, 90. Of cholesterine, 90. Of wax, 92. Of cyanic acid, cyanuric acid, and cyamelide, 92. Of aldehyde, metaldehyde, and elaldehyde, 92. Of proteine, 93. Of albumen from the yolk and white of egg, 93. Of lactic acid, 93. Of gas from the stomach of cows after eating to ex- cess, 93. Of gas from stomach and intestines of executed criminals, 93. Of gelatinous tis- sues, 94. Of tissues containing chondrine, 95. Of arterial membrane, 95. Of horny tissues, 95. Of the lining membrane of the egg, 95. Of feathers, 95. Of the pigmentum nigrum, 95, Of choleic acid, 96. Of taurine, 96. Of cho- loidic acid, 96. Of cholic acid, 98. Of uric acid, 98. Of alloxan, 98. Of urea, 98. Of hippuric acid, 98. Of allantoine, 98. Of xan- thic oxide, 99. Of cystic oxide, 99. Of ox- alic acid, 99. Of oxaluric acid, 99. Of para banic acid, 99. Of roasted flesh, 100. Of Jithofellic acid, 100. Of solanine, 100. Of picrotoxine, 100. Of quinine, 100. Of moi- phia, 101. Of caffeine, theine, or guaranine, 101. Of theobromine, 101. Of asparagine, 101. Animal Heat. Derived from the combination of oxygen with the carbon and hydrogen of the metamorphosed tissues, \\hich proceed ulti- mately from the food, 15. Is highest in those animals whose respiration is most active, 15. Is the same in man in all climates, 15, 16. Is kept up by the food in proportion to amount of external cooling, 16. Is not produced either by any direct influence of the nerves, or by muscular contractions, 18, 19. Its amount in man, 19. Chemical action the sole source of it, 20. The formation of fat from starch or sugar must produce heat, 34. The elements of the bile, by combining with oxygen, serve chielly to produce it, 26. Animal Life. Distinguished from vegetable life by the absorption of oxygen, and the produc- tion of carbonic acid, 11. Must not be con- founded with consciousness, 12. Conditions necessary to animal life, 13, 14. Depends on an equilibrium between waste and supply, 72, 74, 75. Antiseptics. They act by putting a stop to fer- mentation, putrefaction, or other forms of meta- morphosis, 54. Their action on wounds and ulcers, 41. Arteries. Composition of their tunica media, 95. How derived from proteine, 42. Arterial Blood. Conveys oxygen to every part of the body, 26, 77. Contains a compound of iron, most probably peroxide, 77. Yields oxygen in passing through the capillaries, 26, 79. Con- tains carbonic acid dissolved or combined with soda, 79. Asparagine. Its composition, 101. Its relation to taurine and bile, 56. Theory of its action on the bile, 57. Assimilation. In animals it is independent of ex- ternal influences, 11. Depends on the presence in the blood of compounds of proteine, such as fibrine, albumen, or caseine, 21. Is more ener- getic in the young than in the adult animal, 27. Is also more energetic in the herbivora than, in the carnivora, 31. Atmosphere. See Air. Azotized Products. Of vegetable life, 55 57. Of the metamorphosis of tissues. Necessary for the formation of bile in the herbivora, 51. In man, 53. May be replaced by azotized ve- getable compounds, 54. Theory of this, 56 57. Of the transformation of the bile, or of choleic acid ; how related to the constituents of urine, 50. B. Beans. Composition of, 83. Beer. Forms part of the diet of soldiers in Ger many, 83, 85. INDEX. 105 Bees. Their power of forming wax from honey, 9092. Benzoic Acid. See Acid, Benzoic. Berthollet. His analysis of oxalic acid, 99. Berzelius. His analysis of potato starch, 88 ; of sugar of milk, 89 ; of gum, 89 ; of cane sugar, 90. Bezoar stones. See Acid, Lithofellic. Blanchet. Hw analysis of solanine, 100. Bile. In the carnivora is a product of the meta- morphosis of the tissues, along with urate of ammonia, 44. May be represented by choleate of soda, "with which, however, it is not identi- cal, 97. Products of its transformation, 44, 97. Remarks on these, 96 97. Origin of bile, 26, 46. Starch, &c., contribute to its formation in the herbivora, 47, 48, 51, 53. Soda essential to it, 49, 52. Relation of bile to urine, 50. To starch, 51. To fibrine, 44. To caffeine, &c., asparagine, and theobromine, 57. For the acid substances derived from bile, choleic, choloidic, and cholic acids, see Acid, Choleic, &c. Yields taurine, 44. Contains cholesterine, 32, 97. Also stearic and mar- garic acids, 97. Its function: to support respiration and produce animal heat by pre- senting carbon and hydrogen in a very soluble form to the oxygen of the arterial blood, 26, 27. Amount secreted by the dog, the horse, and man, 27. It returns entirely into the circula- tion, and disappears completely, 26, 27. Blood. The fluid from which every part of the body is formed, 13. Its chief constituents, 21. How formed from vegetable food, 22. Can only be formed from compounds of proteine, 23. Is therefore entirely derived from vegetable pro- ducts in the herbivora, and indirectly also by the camivora, which feed on the flesh of the former, 23. Its composition identical with that of flesh, 44. Analysis of both, 96. The se- cretions contain all the elements of the blood, 43. Its relation to bile and urine, 44. Pro- ducts of the oxidation of blood, 45. Excess of azotized food produces fulness of blood and dis- ease, 47. Soda is present in the blood, 52. Important properties of the blood, 5455. Venous blood contains iron, probably as pro- toxide ; arterial blood, probably as peroxide, 79. Theory of the poisonous action of sulphuretted hydrogen and prussic acid: they decompose the compound of iron in the blood, 79. The blood, in analogous morbid states, ought to be chemically examined, 80. Blood-letting. Theory of its mode of action, 78. It may produce opposite effects in different cases, 77. Boeckmann. His analysis of black bread, 83; of potatoes, 83; of dry beef, 96; of dry blood, 96; of roasted flesh, 100. Bones. Phosphoric acid of the food retained to assist in forming them, 31. Gelatine of bones digested by dogs, 35. See, further, Gelatine. Cause of brittleness in bones, 36. Boussingault. His analysis of potatoes, 83. His comparison of the food and excretions in the horse and cow, Table, 86. His analysis of gluten, 87; of vegetable albumen, 87; of ve- getable caseine, 88 ; of oats, 89 ; of hay, 89. Braconnot. On the presence of lactic acid in gastric juice, 38; of iron in the gastric juice of the dog, 38. Brain. See Acid, Cerebric, and Nervous Matter. 14 Bread. Analysis of, 83. Brund. His analysis of sugar of milk, 89. Buckwheat. Analysis of starch from, 88. Burdach. His statement of the amount of bile secreted by animals, 27. Butter. Forms a part of the food of soldiers in Germany, 83, 84. Buzzard. Its excrements consist of urate of am- monia, 24. C. Caffeine. Identical with theine, 56. Its relation to taurine and bile, 56. Theory of its mode of action, 57. Its composition, 101. Cane Sugar. Its composition, 90. Carbon. Is accumulated in the bile, 21. Is given off as carbonic acid, 14. Excess of carbon causes hepatic diseases, 17. By combining with oxygen, it yields the greater part of the animal heat. See Animal Heat, Bile, and Acid, Carbonic. Amount of carbon oxidized daily in the body of a man, 14. Calculations on which this statement is founded, 82 85. Amount consumed by the horse and cow, 14. Different proportions of carbon in different kinds of food, 15. Carbon of flesh compared with that of starch, showing the advantage of a mixed diet, 30. Calculation on which this statement is founded, 89. Amount of carbon in dry blood calculated, 82. Amount in the food of prisoners calculated, 87. Carbonic Acid. See Acid, Carbonic. Carbonates. They occur in the blood, 21. Calculus, Mulberry. Derived from the imperfect oxidation of uric acid, 45. Uric acid calculus is formed in consequence of deficiency of in- Aspired oxygen, or excess of carbon in the food, 45. See Acid, Uric. Bezoar stones composed of lithofellic acid, 49. Carnivora. Their nutrition the most simple, 22. It is ultimately derived from vegetables, 23. Their young, like graminivora, require non- azotized compounds in their food, 23. Their bile is formed from the metamorphosis of their tissues, 25, 26. The process of assimilation in adult and young carnivora compared, 27. Their urine, 30. The assimilative process in adult carnivora less energetic than in graminivora, 31. They are destitute of fat, 31. They swallow less air with their food than graminivora, 40. Concretions of uric acid are never found in them, 47. Both soda and ammonia found in their urine, 52. Caseine. One of the azotized nutritious products of vegetable life, 22. Abundant in leguminous plants, 22. Identical in organic composition with fibrine and albumen, 22, 23. Animal caseine found in milk and cheese; identical with vegetable caseine, 23. Furnishes blood to the young animal, 24. Is one of the piastic elements of nutrition, 35. Yields proteine, 37. Its relation to proteine, 42. It contains sul- phur, 42. Potash essential to its production, 52. Contains more of the earth of bones than blood does, 24. Its analysis, 88. Cerebric Acid. See Acid, Cerebric. Change of Matter. See Metamorphosis of Tissue*. Chemical Attraction. See Affinity. Chevreul. His researches on fat, 32. His ana lysis of fat, 90 ; of cholesterine, 90. Chloride of Sodium. See Common Salt Choleic Acid. See Acid, Choleic. 106 INDEX. Cholesterine. See Bile. Cholic Acid. See Acid, Cholic. Choloidic Acid. See Acid, Choloidic. Chondrine. Its relation to proteine, 42. Ana- lysis of tissues containing it, 95. Chronic Diseases. The action of inspired oxy- gen is the cause of death in them, 17, 18. Chyle. When it has reached the thoracic duct, it is alkaline, and contains albumen coagulable by heat, 47. Chyme. It is formed independently of the vital force, by a chemical transformation, 37. The substance which causes this transformation is derived from the living membrane of the sto- mach, 37. Chyme is acid, 47. Clothing. Warm clothing is a substitute for food to a certain extent, 16. \Vant of clothing ac- celerates the rate of cooling, and the respira- tions, and thus increases the appetite, 16. Cold. Increases the appetite by accelerating the respiration, 16. Is most judiciously employed as a remedy in cerebral inflammation, 76. Concretions. See Calculus, and Acid, Uric; also Acid, Lithofellic. Constituents, Azotized. Of blood: see Fibrine and Albumen. Of vegetables: See Fibrine, Vegetable; Albumen, Vegetable; Caseine, Ve- getable; Alkalies, Vegetable; and Caffeine. Of bile: see Acid, Choleic, Cholic, and Cho- loidic. Of urine: see Acid, Uric; Urea, and Allantoine. Cooling. See Cold and Clothing. Couerbe. His analysis of cholesterine, 90. Cow. Amount of carbon expired by the, 14. Comparison of the food with the excretions of the cow, 86. Crum. His analysis of cane sugar, 90. Cultivation. Is the economy of force, 30. Cyamelide. Its formula, 81. Cyanic Acid. See Acid, Cyanic. Cyanide of Iron/ Its remarkable properties, 78. Cyanuric Acid. See Acid, Cyanuric. D. Davy. Oxygen consumed by an adult man, 82. Death. Cause of, in chronic diseases, 17, 18. Caused in old people by a slight depression of temperature, 75. Definition of it, 74. Demargay. His analysis of choleic acid, choloidic acid, and taurine, 96. Remarks on his Re- searches on Bile, 97. Denis. His experiments on the conversion of fibrine into albumen, 21. Despretz. His calculation of the heat developed in the combustion of carbon, 19. Diabetes Mellitus. The sugar found in the urine in this disease is grape sugar, and is derived from the starch of the food, 35. Diastase. Analogy between its solvent action on starch, and that of the gastric juice on coagu- lated albumen, 38. Diffusion of Gases. Explains the fact that nitro- gen is given out through the skin of animals, 40; and the poisonous action of feather-white wine, 39. Digestion. Is effected without the aid of the vital force, by a metamorphosis derived from the transformation of a substance proceeding from the lining membrane of the stomach, 37. The oxygen introduced with the saliva assists in the process, 38, Lactic acid has no share in it, 38. Disease. Theory of, 74 et seq. Cause of death in chronic disease, 17. Disease of liver caused by excess of carbon or deficiency of oxygen, 1 6 Prevails in hot weather, 17. Dog. Amount of bile secreted by, 27. Digests the gelatine of bones, 35. His excrements con- tain only bone earth, 36. Concretion of urate of ammonia said to have been found by Las- saigne in a dog, doubtful, 47 (note.) Dumas. His analysis of choleic acid, 96; of choloidic acid, 96; of taurine, ib.\ of cholic acid, 97 ; of hippuric acid, 98. E. Eggs. Albumen of the white and of the yolk identical, 37 Analysis of both, 93 ; of lining membrane, 95. The fat of the yolk may con- tribute to the formation of nervous matter, 37. This fat contains iron, 37. Elaldehyde. See Aldehyde. Elements. Of nutrition, 35. Of respiration, 35. Empyreumatics. They check transformations, 54. Their action on ulcers, 41. Equilibrium. Between waste and supply of mat- ter is the abstract state of health, 74, 78. Transformations occur in compounds in which the chemical forces are in unstable equili- brium, 37. Ettling. His analysis of wax, 92. Ettling and Will, their analysis of lithofellic acid, 100. Excrements. Contain little or no bile in man and in the herbivora, none at all in the dog and other carnivora, 27. Those of the dog are phosphate of lime, 35. Those of serpents are urate of ammonia, 24. Those of birds also contain that salt, 24. Those of the horse and cow compared with their food, 86. Excretions. Contain, with the secretions, the elements of the blood or of the tissues, 43, 44. Those of the horse and cow compared with their food, 86. Bile is not an excretion, 26. F. Faeces. Analysis of, 83. Fat. Theory of its production from starch, when oxygen is deficient, 32 et seq. , from other sub- stances, 32. The formation of fat supplies a new source of oxygen, 33 ; and produces heat, 33 et seq. Maximum of fat, how obtained, 34. Carnivora have no fat, 31. Fat in stall-fed animals, 33. Occurs in some diseases hi the blood, 35. Fat in the women of the East, 36. Composition compared with that of sugar, 32. Analysis of fat, 90. Disappears in starvation, 17. Is an element of respiration, 35. Fattening of Animals. See Fat. Featherwhite Wine. Its poisonous action, 39. Febrile Paroxyism. Definition of, 75. Fehling. His analysis of metaldehyde and elal- dehyde, 92. Fermentation. May be produced by any azotized matter in a state of decomposition, 40. Is ar- rested by empyreumatics, 40. , Is analogous to digestion, 40. Fever. Theory and definition of, 75. Fibre. Muscular. See Flesh. Fibrine. Is an element of nutrition, 35. Animal and vegetable fibrine are identical, 22. Is a compound of proteine, 36. Its relation to pro- teine, 42. Convertible into albumen, 21. Is derived from albumen during incubation, 37. Its analysis, 87, 94. Vegetable fibrine, how obtained, 22. INDEX. 107 Fishes. Yield phosphurettecl hydrogen, 59 (note.} Flesh. Consists cliiefly of fibrine, but, from the mixture of fat and membrane, has the same formula as blood, 44. Analysis of flesh, 96, 100. Amount of carbon in flesh compared with that of starch, 30, 86. Food. Must contain both elements of nutrition and elements of respiration, 35. Nutritious food, strictly speaking, is that alone which is capable of forming blood, 21. Whether derived from animals or from vegetables, nutritious food contains proteine, 22, 37 ct seq. Changes which the food undergoes in the organism of the carnivora, 24 et seq. The food of the herbi- vora always contains starch, sugar, &c., 28. Food, how dissolved, 38 et seq. Azotized food has no direct influence on the formation of uric acid calculus, 45. Effects of superabundant azotized food. 47. Non-azotised food contri- butes to the formation of bile, and thus to respiration, 47 et seq. Salt must be added to the food of herbivora, in order to yield soda for the bile, 52. Caffeine, &c., serve as food for the liver, 59. The vegetable alkalies may be viewed as food for the organs which form the nervous matter, 59. Amount of food con- sumed by soldiers in Germany, 83. Its ana- lysis, 82. Food of the horse and cow com- pared with their excretions, 86. Formulae. Explanation of their use, 81. How reduced to 100 parts, 81. Formulae of albu- men, fibrine, caseine, and animal tissues, 42. Formula of proteine, 41 ; of blood and flesh, 44 ; of fat, 32 ; of cholesterine, 32 ; of aldehyde, acetic acid, oil of bitter almonds, and benzoic acid, 81 ; of cyamelide, cyanic acid, and cyan- uric acid, 81 ; of choleic acid, 44; of choloidic acid and cholic acid, 44 ; of gelatine, 46 ; of hippuric acid, 48 ; of lithofellic acid, 49 ; of taurine, 49 ; of alloxan, 49. See Analysis. Francis. His analysis of picrotoxine, 100. Fremy, Lameyran and Fremy. Their analysis of gas from the abdomen of cows after excess in fresh food, 93. His researches on the brain, 21, 57. Frequency of the pulse and respiration in different animals, 15, 87. Fruits. Contain very little carbon, and hence are adapted for food in hot climates, 15. G. Gas. Analysis of gas from abdomen of cows after excess in fresh food, 39, 93. Analysis of gas from the stomach and intestines of executed criminals, 39, 93. Gastric Juice. Contains no solvent but a sub- stance in a state of metamorphosis, by the pre- sence of which the food is dissolved, 37. Con- tains free acid, 37. Contains no lactic acid, 38. In the dog has been found to contain iron, 38. See Digestion, Chyme, Food. Gay-Lussac and Thenard. Their analysis of starch, 88 ; of sugar of milk, and of gum, 89 ; of cane sugar, 90 ; of wax, 92 ; of oxalic acid, 99. Gelatine. Is derived from proteine, but is no longer a compound of proteine, and cannot form blood, 42 et seq. May serve as food for the gelatinous tissues, and thus spare the sto- mach of convalescents, 35, 43. In starvation the gelatinous tissues remain intact, 35. Its relation to proteine, 42. Its formula, 46. Its analysis, 94, 100. Goebel. His analysis of gum, 89. Globules of the blood are the carriers of oxygen to all parts of the body, 54 55. They con- tain iron, 77 et seq. Gluten. Contains vegetable fibrine, 22. Ana- lysis of it, 87. Gmelin. On the sugar of bile, 47. Goose. How fattened to the utmost, 34. Graminivora. See Herbivora. Grape-sugar. An clement of respiration, 35. Is identical with starch sugar and diabetic sugar, 29. Its composition, 29. Its analysis, 88. Growth, or increase of mass, greater in gramini- vora than in carnivora, 31. Depends on the blood, 21 ; and on compounds of proteine, 37. See Nutrition. Gum. An element of respiration, 36. Its com- position, 35. Is related to sugar of milk, 35. Its analysis, 89. Gundlach. His researches on the formation of of wax from honey of the bee, 91. H. Hair. Analysis of, 95. Its relation to proteine, 42. Analysis of proteine from hair, 93. Hay. Analysis of, 89. Hepatic Diseases. Cause of, 16. Herbivora. Their blood derived from compounds of proteine in their food, 23. But they require also for their support non-azotized substances, 28. These last assist in the formation of their bile, 47 et seq. They retain the phosphoric acid of their food to form bone and nervous matter, 31. Their urine contains very little phosphoric acid, 31. The energy of vegetative life in them is very great, 31. They become fat when stall-fed, 31. Hess. His analysis of wax, 93. Hybernating Animals. Their fat disappears dur- ing the winter sleep, 17. They secrete bile and urine during the same period, 26. Hippuric Acid. See Acid, Hippuric. Horn. Analysis of, 95. Contains proteine ; its relation to proteine, 42. Analysis of proteine from horn, 93. Horse. Amount of carbon expired by, 14. Com' parison of his food with his excretions, 86. Force exerted by a horse in mechanical motion, compared to that exerted by a whale, 70. Hydrocyanic Acid. See Acid, Hydrocyanic. Hydrogen. By combining with oxygen contn butes to produce the animal heat, 17. I. Ice. Is judiciously . employed as a remedy in cerebral inflammation, 76. Inorganic constituents of albumen, fibrine, and caseine, 21, 41, 42. Jobst His analysis of theine, 101. Jones, Dr. Bence. His analysis of vegetable fibrine, 86 ; of vegetable albumen, 87 ; of ve- getable caseine, 87; of gluten, 87; of the albu- men of yolk of egg, 93, 94 ; of the albumen of brain, 94. Iron. Is an essential constituent of the globules of the blood, 77 et seq. Is found in the fat of yolk of egg, 3T. Also in the gastric juice of the dog, 38. Singular properties of its com- pounds, 78. Isomeric Bodies, 36, 81. K. Keller. His researches on the conversion of benzoic acid into hippuric acid in the human body, 101. 108 [NDEX. Kidneys. They separate from the arterial blood the nitrogenized compounds destined for excre- tion, 49. L. Lactic Acid. See Acid, Lactic. Lavoisier. His calculation of the amount of in- spired oxygen, 14, 81. Lehmann. On the presence of lactic acid in gastric juice, 38. Liebig. His analysis of sugar of milk, 89 ; of cane sugar, 90 ; of aldehyde, 92; of uric acid, 97; of hippuric acid, 98 ; of quinine, 100; of morphia, 101; of asparagine, 101. His calcu- lation of the carbon daily expired as carbonic acid, 14, 82. Table, 84. His remarks on Demarc.ay's researches on bile, 96, 97. Liebig and PfafT. Their analysis of caffeine, 101. Liebig and Wohler. Their analysis of alloxan, 98 ; of urea, 98 ; of allantoine, 98 ; of xanthic oxide, 99 ; of oxaluric acid, 99 ; of parabanic acid, 99. Lentils. Contain vegetable caseine, 22. Ana- lysis of, 82, 83. Form part of the diet of sol- diers in Germany, 83. Table, 85. Light. Its influence on vegetable life analogous to that of heat on animal life, 69. Lime. Phosphate of. See Bones. Liver. It separates from the venous blood the carbonized constituents destined for respiration, 25. Diseases of the liver, how produced, 1 6. Accumulation of fat in the liver of the goose, 35. M. Maize. Analysis of starch from, 88. Marchand. On the amount of urea in the urine of the dog when fed on sugar, 26. His ana- lysis of cholesterine, 90. Marcet. His analysis of gluten, 87. ' Martius. His analysis of guaranine, 101. Mechanical Effects. See Motion. Medicine. Definition of the objects of, 75 et seq, Action of medicinal agents, 54 et seq. Menzies. His calculation of the amount of in- spired oxygen, 14, 81. Metaldehyde. See Aldehyde. Metamorphosis of Tissues, 36 et seq. In other parts of the volume, passim. Milk. Is the only natural product perfectly fitted to sustain life, 23. Contains caseine, 23. Fat (butter), 23. Sugar of milk, 23. Earth of bones, 23. And potash, 52. Morphia. Contains less nitrogen than quinine, 56. Its analysis, 101. Mitscherlich-. His analysis of uric acid, 96 ; of hippuric acid, 96. Momentum. Of force, 61. Of motion, 61. Motion. Phenomena of motion in the animal body, 60 et seq. Different sources of motion, 60. Momentum of motion, 61. Motion pro- pagated by nerves, 60. Voluntary and invo- luntary motions accompanied by a change of form and structure in living parts, 66. Motion derived from change of matter, 66 et seq. The cause of motion in the animal body is a peculiar force, 69. The sum of the effects of motion in the body proportional to the amount of nitrogen in the urine, 72. Mulberry Calculus. See Calculus. Mulder. Discovered proteine, 36. His analysis of fibrine of blood, 87. Of animal caseine, 88. Of proteine, 88. Of fibrine, 94. Of gelatine, 04. Of chondrine, 95. Muscle. See Flesh. Muscular Fibre. Its transformation depends on the amount of force expended in producing motion, 66. N. Nerves. Are the conductors of the vital force, and of mechanical effects, 66. Effects of the disturbance of their conduting power, 68. They are not the source of animal heat, 18. Nervous Life. Distinguished from vegetative, 20. Nervous Matter. Contains albumen, and fatty matter of a peculiar kind, 21. Vegetables can- not produce it, 23. The fat of yolk of egg probably contributes to its formation, 37. The phosphoric acid and phosphates, formed in the metamorphosis of the tissues of the herbivora, are retained to assist in the formation of nervous matter, 31. The vegetable alkalies affect the nervous system, 57. Composition of cerebric acid. Theory of the action of the vegetable alkalies, 58. Nitrogen. Essential to all organized structures, 21. Substances in the body which are destitute of it not organized, 21. Abounds in nutritious vegetables, 22, Nutritious forms in which it occurs, 22 et seq. Occurs in all vegetable poi- sons, 56 ; also in a few substances which are neither nutritious nor poisonous, but have a peculiar effect on the system, such as caffeine, 56 et seq. Nitrogenized. See Azotized. Non-Azotized. Constituents of food. See Starch. Nutrition. Depends on the blood, 21. On Albu- men, fibrine, or caseine, 21 et seq. Elements of nutrition, 35. Compounds of proteine alone are nutritious, 37. Occurs when the vital force is more powerful than the opposing chemical forces, 60. Theory of it, 63. Is almost unli- mited in plants from the absence of nerves, 64. Depends on the momentum of force in each part, 68. Depends also on heat, 72. O. Oats. Amount required to keep a horse in good condition, 29. Analysis of, 89. Oil of Bitter Almonds. Its composition. How related to benzoic acid, 81. Old Age. Characteristics of, 73 et seq. Oppermann. His analysis of wax, 92. Organs. The food of animals always consist of parts of organs, 11. All organs in the body contain nitrogen, 21. There must exist organs for the production of nervous matter, 59 ; and the vegetable alkalies may be viewed as food for these organs, 59. Organized Tissues. All contain nitrogen, 21. All such as are destined for effecting the change of matter are full of small vessels, 67. Their composition, 42. The gelatinous and cellular tissues, and the uterus, not being destined for that purpose, are differently constructed, 67. Waste of organized tissues rapid in carnivora, 30. Origin. Of animal heat, 15, 18. Of fat, 31 et seq. Of the nitrogen exhaled from the lungs, 39 et seq. Of gelatine, 42 et seq., 48. Of uric acid and urea, 44 et seq. Of bile, 44, 47, 48 et seq. Of hippuric acid, 48, 101. Of the chief secretions and excretions, 49. Of the soda of the bile, 52 et seq. Of the nitrogen in bile, 53. Of nervous matter, 57 et seq. Ortigosa. His analysis of starch, 88. INDEX. 109 Oxalic Acid. A product, along with urea, of the partial oxidation of uric acid, occurring in the form of mulberry calculus, 45. Its analysis, 99. Oxygen. Amount consumed by man daily, 14, 80. Amount consumed daily in oxidizing car- bon by the horse and cow, 14. The absorption of oxygen characterizes animal life, 11. The action of oxygen is the cause of death in star- vation and in chronic diseases, 17 18. The amount of oxygen inspired varies with the tem- perature, dry ness, and density of the air, 15. Is carried by arterial blood to all parts of the body, 54. Fat differs from sugar and starch only in the amount of oxygen, 32. It also contains less oxygen than albumen, fibrine, &c., 32. The formation of fat depends on a defi- ciency of oxygen, 33 et seq. , and helps to sup- ply this deficiency, 33. Oxygen essential to digestion, 38. Relation of oxygen to some of the tissues formed from proteine, 42. Oxygen and water, added to blood or to flesh, yield the elements of bile and of urine, 44. Action of oxygen on uric acid, 44, 45 : on hippuric acid, 31,45; on blood, 45; on proteine, with uric acid, 48 ; on proteine and starch, with water, 49 ; on choleic acid, 49 ; on proteine, with water, 49. By depriving starch of oxygen and water, choloidic acid may be formed, 51. Oxygen is essential to the change of matter. 55. Its action on the azotized constituents of plants when separated, 64. Its action on the muscular fibre essential to the production of force, 66, 67. Oxygen is absorbed by hybernating animals, 71. Is the cause of the waste of matter, 72 ; and of animal heat, 72, 74. Blood-letting acts by diminishing the amount of oxygen which acts on the body, 75. Its absorption is the cause of the change of colour from venous to arterial blood, 77. The globules probably contain oxide of iron, protoxide in venous blood, peroxide in arterial, 78 et seq. All parts of the arterial blood contain oxygen, 55, 77, 79. P. Pears. Analysis of starch from unripe, 88. Peas. Form part of the diet of soldiers in Ger- many, 83, 85. Abound in vegetable caseine, 22. Analysis of peas, 83 ; of starch from peas, 88. Pepys and Allen. Their calculation of the amount of inspired oxygen, 82. Peroxide of Iron. Probably exists in arterial blood, 78 et seq. Pfluger. His analysis of the gas obtained by puncture from the abdomen of cattle after ex- cess in green food, 93. Phenomena of motion in the animal body, 60 et seq. Phosphates. See Bones. Phosphoric Acid. See Acid, Phosphoric. Phosphorus. Exists in albumen and fibrine, 21, 23, 42. It is not known in what form, 41 et seq. Is an essential constituent of nervous mat- ter, 57, 59. Phosphuretted Hydrogen. Occurs among the pro- ducts of the putrefaction of fishes, 59. Picrotoxine. Contains nitrogen, 56 (no/e.) Its analysis, 100. Plants. Distinguished from animals by fixing carbon and giving out oxygen, 11, 64; by the want of nerves and of locomotive powers, 11. Their capacity of growth almost unlimited, 64, Cause of death in plants, 64. Playfair, Dr. L. His formula for blood, 38. His analysis of faeces, of peas, of lentils, of beans, 82 ; of flesh and of blood, 96 ; of roasted flesh, 100. Poisons, Vegetable. Always contain nitrogen, 55 et seq. Different kinds of poisons, 54. Theory of the action of prussic acid and sul- phuretted hydrogen, 80. Polymeric Bodies, 36. Potash. Essential to the production of caseine 01 milk, 52. Potatoes. Amount of carbon in, 83. They form part of the diet of soldiers in Germany, 83. Analysis of, 83 ; of starch from, 83 ; of sola- nine from the buds of germinating potatoes, 100. Prevost and Dumas. On the frequency of the pulse and respirations, 86. Products. Of the metamorphosis of tissues found in the bile and urine, 43. Of the action of muriatic acid on bile, 44. Of the action of potash on bile, 44. Of the action of water and oxygen on blood or fibre, 44. Of the oxidation of uric acid, 45. Of the oxidation of blood, 45 . Of the action of water on proteine, 46. Of the action of urea on lactic and benzoic acids, 48 . Of oxygen and uric acid on proteine, 48. Of oxygen on starch and hippuric acid, 48. Of oxygen and water on proteine and starch, 49. Of oxygen and water on proteine when soda is absent, 49. Of the separation of oxygen from starch, 50. Of the action of water on urea, 51. Of the action of water and oxygen on caffeine or theine, asparagine, and theobromine, 56. Proteine. Discovered by Mulder, 36. Its com- position, 36. Produced alone by vegetables, 37. Is the source of all the organic azotized consti- tuents of the body, 37. Its formula, 41. Its relation to fibrine, albumen, caseine, and all the animal tissues, 42. Gelatine no longer yields it, although formed from it, 43. Its relation to bile and urine, 44. Its relation to allantoine and choloidic acid, 46 ; to gelatine, 46 ; to hip. puric acid, 48 ; to the chief secretions and ex- cretions, 48, 49 ; to fat, 49 Analysis of pro- teine from the crystalline lens, from albumen, from fibrine, from hair, from horn, from vegeta- ble albumen and fibrine, from cheese, 92. Prout. His analysis of starch, 88 ; of grape su- gar from honey, 88 ; of sugar of milk, 88 ; of cane sugar, 89 ; of urea, 90. His discover/ of free muriatic acid in the gastric juice, 38. On the effect of fat food on the urine, 45. Prussic Acid. See Acid, Hydrocyanic. Pulmonary Diseases. Arise from excess of oxy- gen, 16. Prevail in winter, 17. Pulse. Its frequency in different animals, 86. Putrefaction. Is a process of transformation, 37. Membranes very liable to it, 38. Effects of the putrefaction of green food in the stomach of animals, 39. Is analogous to digestion, 40. Putrefying animal matters cause the fermenta- tion of sugar, 40. Is checked by empyreuma- tics, 41, 54. Q. Quinine. Contains nitrogen, 56. Its analysis, 100. R. Regnault His analysis of morphia, 101. Reproduction of Tissues. See Nutrition. R 110 INDEX. Reproduction of the Species, 20. Rhenish Wines. Contain so much tartar, that their use prevents the formation of uric acid calculus, 49. Respiration. Theory of, 77 et seg. Its connexion with the food and with animal heat, 14 et sea. S. Salt, Common. Essential to the formation of bile in the herbivora, and to that of gastric juice, 52 et seq. Saussure, De. His analysis of grape sugar and of starch sugar, 88, of wax, 92. Scherer, Dr. Jos. His analysis of albumen from serum of blood, 87, of fibrine of blood, 87, of vegetable fibrine, 87, of vegetable caseine, 88, of animal caseine, 88, of proteine from differ- cnt sources, 92, of albumen from white of egg, 92, of albumen from different sources, 94, of fibrine, 94, of gelatine from different sources, 94, of tissues containing chondrine, 95, of the tunica media of arteries, 95, of horny tissues, 95, of the lining membrane of the egg, 95, of feathers, 95, of the pigmentum nigrum oculi, 95. Results of his researches, 42. Secretions. See Bile and Urine. Seguin. His calculation of the amount of inspired oxygen, 80. Serpents. Their excrements consist of urate of ammonia, 24. The process of digestion in them, 24. Sleep, Theory of, 68. Amount of sleep necessary for the adult, the infant, and the old man, 73 et seq. Induced by alcohol or wine, 71. Soda. Essential to blood and bile, and derived from common salt, 76 et seg. Sodium, Chloride of. See Salt. Solanine. Contains nitrogen, 56. Its analysis, 100. Starch. Exists in the food of the herbivora, 28. Is convertible into sugar, 28, 29. Its relation to gum and sugar, 29. Its function in food, 29 et seg. Amount of carbon in starch compared with that in flesh, 30. Its composition com- pared with that of fat, 32, 33. Is the source of diabetic sugar, 35. Is an element of respi- ration, 35. Dissolved by diastase, 38. Its re- lation to choleic acid, 48. Its relation to the principal secretions and excretions, 49, to cho- loidic acid, 51, to bile, 51, 52, 53. Its analysis from fifteen different plants, 88. Starvation. Process of, 17. Cause of death in, 17. Strecker. His analysis of starch from twelve dif- ferent plants, 88. Sugar. Analysis of grape-sugar, 88, of sugar of milk, 89, of cane sugar, 90. Is an element of respiration, 35. Sulphur. Exists in albumen, and caseine, 21, 42. Sulphuretted Hydrogen. Theory of its poison- ous action, 80. Sulphuric Acid. See Acid, Sulphuric. Supply of matter. See Nutrition. Supply and Waste. Equilibrium between them constitutes the abstract state of health, 74, 75. Effects of its disturbance, 75 et seq. Means for restoring the equilibrium, 73, 75 et seq. . T. Tables of the food consumed by soldiers of Ger- many, 83. Of the food and excretions of the horse and cow, 86. Taurine. How produced from bile, 44. Its re- lation to choleic acid, 44. Its relation to uric acid and urea, and to allantoine, 49, to uric acid 50, to alloxan, 50, to choloidic and choleic acids, and ammonia, 51, to caffeine or theine, 56, to asparagine, 56, to theobromine, 57. Temperature. Its effects on the amount of in spired oxygen, 15, and on the appetite, 15 et seg. A slight depression of temperature causes death in aged people, 75. Temperature of the blood in different animals, 87. Temperature of the body constantly kept up by internal causes, 15, 16. Tendons. Analysis of, 94. Thaulow. His analysis of cystic oxide, 99. Theine. Is identical with caffeine, 56. And with guaranine, 57. Theory of its action, 57 et seg. Its relation to bile, 56. Its analysis, 101. Theobromine. Analogous to theine, 56. Theory of its action, 57 et seg. Its relation to bile, 56, 57. Its analysis, 101. Theory. Of animal heat, 15 et seq. Of diges- tion, 37 et seg. Of respiration, 77 et seg. Of the motions in the animal organism, 60 et seg. Of disease, 74 et seg. Of the action of caffeine, &c., 57 et seq. Of the action of the vegetable alkalies, 57 et seg. Of health, 74, 75. Tiedemann and Gmelin. Their attempt to sup- port a goose upon albumen alone, unsuccessful, 37. Tissues, Metamorphosis of: see Metamorphosis. Analysis of the animal tissues, 94, 95. Formu- las of, 42. Tobacco. Arrests or retards the change of matter, 56. Transformation. See Metamorphosis. Turnips. Juice of, contains vegetable fibrine and albumen, 22. U. Urea. Derived from uric acid, 45. Also from the oxidation of blood, 45 ; from allantoine, 16. Its relation to choleic acid, 48; to hippuric acid, 48; to proteine, 48; to proteine and starch, 49 ; to proteine .and fat, 49 ; to taurine, 50; to carbonate of ammonia, 51 ; to theobro- mine, 56. Its analysis, 98. Occurs in the urine of those who have taken benzoic acid along with hippuric acid, 102. Urinary Calculi. See Calculus, Uric Acid. See Acid, Uric. V. Varrentrapp and Will. Their analysis of ve- getable albumen, 87. Of sulphate of potash and caseine, 88. Vegetables. Alone produce compounds of pro- teinc, 37. Azotized constituents of, nutritious, 22: medical or poisonous, 55. Analysis of those vegetables which are used for food, 82 et seg. Vegetable Life. Distinguished from nervous life, 20. . Predominates in the early stages of life, 20. Also in the female, 20. Venous Blood. See Blood. Vital force, or vitality. Definition of, 11 et seq. Theory of, 60 et seg. Vogel. His analysis of gas from the abdomen of cattle after excess in green food, 93. W. Water. Is one of the two constituents of the body which contain no nitrogen, 21. Its use as a solvent, 21. Contributes to the greater part of the transformations in the body, 44 57. INDEX. lii Wax. On its production from honey by the bee, 90 92. Its analysis, 92. Wheat. Contains vegetable fibrine, 22. Ana- lysis of fibrine, albumen, and gluten, from wheat, 87. Will and Ettling. acid, 100. Their analysis of lithofellic Wine. The wines of the south promote the formation of calculus, 45. But not Rhenish wines, 45. Theory of its action, 72. Woskresensky. His analysis of theobromine, 101. Y. Yams. Analysis of starch from, 88. 14 DAY USE URN TO DESK FROM WHICH BORROWED LOAN DEPT. * I*** "due on the last date stamped below, !? if *"' to which renewed. books are subject to immediate recall. or MAR 2 4 1975 7 9 LD 2lA-40m-ll '63 (El602slO)476B .General Library University of California Berkeley