M . : f Agricultural f r\ c r a i i c r\ d m i a I |*i it If f H. Z. HIELD R. M. BURNS C. W. COGGINS, JR. PRE HARVEST USE OF ON CITRUS !» (h n Hi LLU is the popular name for 2,4-dichlorophenoxyacetic acid, a plant growth regulator of proved value to the citrus industry. When properly used, 2,4-D can reduce pre-harvest fruit drop, increase fruit size, reduce leaf and fruit drop following pesticide oil sprays, and lessen fruit stem die-back. This circular tells how to use 2,4-D on citrus. MAY, 1964 The Authors: Henry Z. Hield is Specialist in the Citrus Research Center and Agricultural Experi- ment Station, Riverside; Robert M. Burns is Extension Horticulture Technologist, Riverside; Charles W. Coggins, Jr., is Assistant Plant Physiologist in the Citrus Research Center and Agricultural Experiment Station, Riverside. PRE HARVEST USE OF ON CITRUS H.Z.HIELD • R.M.BURNS • C. W. COGGINS, JR. Dlant growth regulators such as 2,4-D cause a change in normal plant proc- esses when used in small amounts. In cit- rus spraying, the type of 2,4-D used is of vital importance as there are many types marketed for weed killing and some of them will cause damage to citrus at con- centrations used for growth regulator purposes. The only form of 2,4-D which should be applied to citrus in California is the high-volatile ester, which is labeled as a citrus growth regulator. CONTROLLING FRUIT DROP WITH 2,4-D When used as recommended on nearly- mature to mature fruit, sprays of 2,4-D may be expected to reduce drop about 50 per cent. These "drop-spray" treatments become effective approximately 1 week after application. Regulator sprays reduce the drop of some unsound as well as sound fruit, and this leads to high cullage in the packing house if a large percentage of un- desirable fruit is present in the orchard. The maximum period of effectiveness of a drop spray is 3 or 4 months. Sprays applied while fruit has a partially green rind may delay development of color for about 10 days and therefore 2,4-D should not be applied to early market orchards. The principal difference in effectiveness of an 8 ppm (parts per million) and 16 ppm concentration of 2,4-D in the spray is the greater period of time over which drop is controlled by the higher concentration. 2,4-D reduces fruit drop mainly because of the effect on the fruit stem; the treated fruit stems remain alive and functional longer because there is delayed develop- ment in the abscission zone (the separa- tion point of fruit and stem). 2,4-D sprays should never be applied to citrus fruit less than 7 days before harvest. Navel Oranges It is estimated that approximately two- thirds of the navel orange orchards in southern California are treated with 2,4-D to reduce fruit drop. Here, the most desir- able treatments are either a 16 ppm water spray in October or November, or an 8 ppm water spray in December or January. In groves where summer oil spray is ap- plied for insect control the inclusion of 4 ppm of 2,4-D will often give partial con- trol of preharvest fruit drop. In the central San Joaquin Valley, where whitewash sprays are applied in October to repel leafhoppers, the inclusion of 24 ppm of 2,4-D will give good mature-fruit drop reductions. Where 2,4-D is not in- cluded in the whitewash, a water 2,4-D spray may be applied at 16 ppm in Octo- ber or November, or at 8 ppm in Decern- ber, provided it is applied at least 2 days prior to, or 2 to 3 weeks following, white- washing. Valencia Oranges Pre-harvest fruit drop reductions may be obtained with 2,4-D spray concentra- tions so low that they will exert no influ- ences on the following year's fruit size. An 8 ppm spray applied when fruit is an aver- age of at least Vi -inch in diameter will re- duce mature-fruit drop but will not cause a fruit-size increase on the next year's crop. A reduction in mature-fruit drop, and an increase in fruit size of the follow- ing crop, may be affected in one spray by following the fruit-sizing procedure de- scribed on page 5. Valencia oranges are generally less sub- ject to severe fruit drop when held late on the tree than are navel oranges and grape- fruit. Grapefruit Reduction of fruit drop in grapefruit requires dosage and timing similar to that necessary for Valencia oranges. A spray of 16 ppm applied in June or July will reduce fruit drop during summer and early fall. The young fruit should average at least % -inch in diameter in order for final size to be unaffected. If fruit-sizing sprays are applied before June or July drop of mature fruit is reduced, but not as much as by drop sprays applied later in the season. In the central San Joaquin Valley a seri- ous winter fruit drop may occur, and a spray of 24 ppm of 2,4-D in an October whitewash, or a water spray of 1 6 ppm in October or November, should be used. Lemons In inland areas of southern California, 4 ppm of 2,4-D in the fall oil-spray appli- cation reduces fruit drop in the early spring. 2,4-D sprays are also often desir- able because they cause a delay in the development of the yellow rind color of the fruit. A water spray of from 8 to 12 ppm applied during November to De- cember will have approximately the same effect on the fruit as 4 ppm of 2,4-D in oil. FRUIT-SIZING SPRAYS Spray formulations of 2,4-D applied to small green fruit are called "sizing sprays." When 2,4-D-treated fruit reach maturity they usually show an average increase of approximately one commercial size class. In oranges this is usually associated with an increase in the thickness and roughness of the rind. This may result in a reduction of from 1 to 2 per cent less fruit in first- grade commercial quality, but for growers having a small-size problem the increase in size more than compensates for the slight decrease in quality. (Juice weight is lowered about 1 per cent, but soluble sol- ids and acid contents are not influenced.) The normal rind texture and size of fruit produced by an orchard should be considered before applying a fruit-sizing spray: if the fruit is rougher than average for the district, 2,4-D sprays should not be applied; if the orchard has a history of Table 1. 2,4-D Concentration for Fruit-Sizing Sprays* Average fruit diameter Concentration of 2,4 D in spray inches Grapefruit: 1/4 to 3/8 ppm 12 3/8 to 5/8 16 5/8 to 3/4 20 3/4 to 1 24 Oranges: 3/16 to 1/4 12 1/4 to 1/2 . 16 1/2 to 5/8 20 5/8 to 3/4 24 * Average fruit diameter in the orchard determines the appropriate concentration to apply. \ , / / "><** % / w i , % #' % 4 111 AVERAGE SIZE Figure 1. Young grapefruit. Note average-sized fruit used to determine fruit-sizing spray concentration. satisfactory-to-good rind texture, but has poor sizes, a sizing spray should be con- sidered. (2,4-D sprays cause a greater de- gree of rind roughness in light-crop years than they do in normal or heavy-crop years.) The proper concentration of 2,4-D is determined by the average diameter of the young fruit (table 1 and figure 1), and a random measurement throughout the or- chard must be made to determine average fruit size. Sprays applied too early during the bloom period may cause excessive fruit thinning, while sprays applied as much as 3 months after bloom will be relatively ineffective in increasing fruit size. i Valencia and Navel Oranges A spray may be applied at any one of the concentrations shown in table 1 with approximately equal effectiveness. Sprays stronger than the 24 ppm concentration are not recommended, not only from a residue standpoint but also because greater concentrations may result in leaf curl in subsequent growth. The responsiveness of the young fruit decreases rapidly with size increase. Fruit size is not as general a problem with the navel as it is with the Valencia orange. Grapefruit The general specifications for sizing sprays on oranges are applicable to grape- t * m 12 16 20 24 ppm WI '1 ' i 'lii i Ji i iiii'i' iii i i yi iiiiiii i i i i i i j, Figure 2. Young navel and Valencia oranges, and appropriate 2,4- D concentrations for a fruit-sizing spray. fruit, although size-concentration catego- ries are different because of the larger fruit. Quality of grapefruit is less affected by growth regulator sprays than is quality of any other common citrus variety, and the response of grapefruit to 2,4-D sprays is not as great as with oranges. Lemons Because response of lemons to fruit- sizing experiments has been variable, 2,4-D sprays are not recommended. ADDITIONAL EFFECTS OF 2,4-D Splitting at the navel end of Washington navel oranges may cause a considerable loss of fruit. Fruit-sizing sprays have sometimes resulted in approximately a 50 per cent reduction in splitting. Current in- formation indicates that 2,4-D has no effect on creasing or puffing of navel or Valencia oranges, and puffing and creas- ing of rinds associated with over-maturity is not delayed by the sprays. Delay of granulation has been observed in Valencia oranges which were sprayed to increase fruit size, but after the initial delay no further effects of the sprays were evident. Fruit stem die-back occurring late in the harvest season is reduced by 2,4-D fruit- holding sprays; this results in more turgid fruit and more fruit at harvest. Pesticide oil sprays occasionally cause leaf and fruit drop. This drop may be re- duced by the inclusion of 2,4-D in the spray oil at a concentration of 4 ppm. 2,4-D has not increased fruit-set on any citrus variety in California. 2,4-D is a potent weed killer and should be handled with care to avoid plant dam- age. This material is of a low level of toxicity to humans, but should never be sprayed over fruit less than 7 days before harvest. The Federal Residue Tolerance for 2,4-D in ctirus is 5 ppm (based on fruit weight). 2,4-D has been registered by the USDA for citrus when used as directed. In growth regulator usage, concentra- tions are approximately one one-hun- dredth of those used in weed killing. The concentrations used on citrus will cause severe damage to some plants, such as cotton, grapes, olives, or tomatoes, and so the effect on adjoining acreage must be carefully considered when planning a spray application. Leaf Injury 2,4-D sprays should be timed to avoid periods of immature leaf growth, as they will cause leaf curl and distortion of young growth even at recommended concentra- tions. Applications after a growth flush has matured and hardened, or before start of new growth, will minimize or eliminate leaf distortion. Tree Damage Using 2,4-D in forms other than the high-volatile ester, or making large errors in calculating proper concentration, has caused severe tree injury. On mature trees, injury has ranged from severe leaf curl and chlorosis to a reduction in the amount of growth during one or two succeeding growth cycles (although these trees later resumed normal growth). Trees less than 6 years old should not be sprayed with 2,4-D. The unfavorable ef- fects on fruit quality and tree growth re- sulting from a spray application to young trees more than offsets any possible bene- fits in fruit size or reduction in fruit drop. Figure 3 (left). Pinched tips and slight cupping of grapefruit leaves 2 to 3 weeks after 2,4-D spraying. Figure 4 (right). Mature grapefruit leaves showing slight buckle resulting from a 2,4-D spray applied when leaves were in a half-expanded and soft state of growth. Similar results occur when spray is applied during a growth flush. HANDLING 2,4-D 2,4-D should not be sprayed from air- craft. The hazard of low volume, high concentration sprays, and the danger of drift to adjoining crops, has resulted in regulations generally prohibiting such application. Once the desired concentration for a ground-spray has been determined it is necessary to determine the strength of the commercial product to be used. The pounds per gallon of acid equivalent is given on the label; this value is used to determine the actual volume to be added to each tank of spray mixture for a given concentration. Table 2 gives data on 500- gallon quantities of spray mixture. The 2,4-D formulation should be pre- measured into small containers, as this precludes possible errors in measuring during the spray operation and eliminates spillage in the field. Small, wide-mouthed bottles are satisfactory, and can be rapidly filled from a large burette or from a graduated cylinder; the amount of 2,4-D required for a tank of a given volume at the desired concentration is placed in one bottle. Containers should be emptied and rinsed directly into the spray tank — a wire basket over the tank-opening eliminates danger of bottles dropping into the tank. Before spraying, the spray hose should be directed on the ground until liquid which has been standing in the hoses is flushed out. Spray mixtures should not stand in equipment, as contamination or precipitation may occur. The small quantities of lime used to neutralize zinc sulfate sprays have not in- fluenced the effectiveness of 2,4-D added to this type of nutritional spray; urea added to citrus 2,4-D sprays has not in- fluenced their effectiveness. Additional emulsifiers or wetting agents are not necessary in the sprays discussed in this circular. Weather Regulator sprays are most effective when applied during warm sunny weather. Absorption of the regulator by leaves is rapid; rainfall 48 hours following appli- cation has not lessened effectiveness. Tree Condition Adequate soil moisture and healthy trees will help assure effectiveness of the spray (trees needing water may not respond). Gallonage Necessary coverage depends upon the purpose of the spray; fruit-sizing applica- tions require more complete wetting of the tree than do sprays designed for mature fruit-drop reduction. Tree size, the presence of other materials in the spray WARNING ON PESTICIDE RESIDUES These recommendations for chemicals are based on the best information cur- rently available. Treatments based upon these recommendations should not leave residues that will exceed the tolerance established for any particular chemical. To avoid excessive residues, follow directions carefully with respect to dosage levels, number of applications, and minimum interval between application and harvest. The grower is legally responsible for residues on his crops as well as for prob- lems caused by drift from his property to other properties or crops. (such as pesticides and nutrients), and the type of spraying equipment used, all in- fluence the gallonage required. Commer- cial spray operators can give accurate estimates of the gallonage required, but in general applications of not less than 500 gallons per acre for fruit sizing and not less than 350 gallons per acre for reducing fruit drop are recommended. Reducing gallonage and increasing the 2,4-D con- centration is not advisable, as damage can result from an uneven distribution of spray applied at concentrations higher than recommended. Compatibility of 2,4-D With Other Chemicals 2,4-D materials are often combined with pesticide and nutritional sprays to eliminate the need for several spray opera- tions. 2,4-D is compatible with the pesti- cide and nutritional materials in common use. (Consult your local Farm Advisor for current spray recommendations if there is a question as to compatibility.) Oil Sprays The ester forms of 2,4-D more readily enter the plant when in an oil solution. Fruit-sizing and fruit-drop spray concen- trations given here are for water-spray mixtures only; therefore, drop or sizing sprays should not be combined with pesti- cide oil sprays. Oil sprays occasionally cause leaf and fruit drop due to oil injury to the plant; this may be reduced by the inclusion of 2,4-D in the spray at concen- trations not exceeding 4 ppm. Recommended amounts of oil-type wet- ting agents required by other materials in a spray mixture have not undesirably in- fluenced the 2,4-D plant response. Whitewash Sprays Whitewash sprays used on citrus in the Central Valley may contain as much as 15 pounds of lime per 100 gallons of spray. This quantity of lime reduces the effec- tiveness of a particular concentration of 2,4-D. Increased 2,4-D concentrations needed to compensate for 2,4-D made in- effective by lime are given on pages 3 and 4. Cleaning Spray Equipment Equipment used for spraying 2,4-D should never be used for spraying 2,4-D- sensitive crops; 2 ,4-D -contaminated weed spray equipment should never be used for tree sprays. 2,4-D sprays should be drained from equipment and hoses when the operation is completed. Residues may be reduced by rinsing with water, refilling with water to stand overnight in the tank, pump and hoses, and then flushing again with clean water. This precaution should be sufficient to safeguard citrus against leaf curl in future spray operations. DOSAGE RECOMMENDATIONS Use a high-volatile ester formulation, the label of which specifies that it is pre- pared for use on citrus. Spray only once and never less than 7 days before harvest. All sprays should be water mixtures unless otherwise specified. Control of Fruit Drop Navel Oranges — Southern California (1) 16 ppm in November or December, or (2)8 ppm in January, or (3)4 ppm in summer oil spray. This spray may not give good fruit-drop reductions late in the season. Navel Oranges — Central California Do not spray early-maturity orchards. ( 1 ) 24 ppm in October whitewash spray, or (2) 16 ppm in October or November, or (3)8 ppm in December. Valencia Oranges — Southern California (1)8 ppm when young fruit of following year's crop is at least Vi-inch in diameter, or (2) A combination fruit-drop fruit-sizing spray following the fruit-sizing procedure. Valencia Oranges — Central California Here, Valencias are usually picked relatively early in the season and drop is seldom a problem. Grapefruit — Winter Fruit Drop — Central California (1 ) 24 ppm in October Whitewash spray, or (2) 16 ppm in October or November. Grapefruit — Summer Fruit Drop — Southern and Central California (1) 16 ppm when young fruit of the next year's crop is at least 3 /4-inch in diameter, or (2) In a combination fruit-drop and sizing spray following the fruit-sizing procedure. Lemons — Southern California (inland) (1)4 ppm in a summer or fall oil spray, or (2) 8 ppm in November or December. Fruit-Sizing Sprays Oranges and Grapefruit — Southern California Spray only once, using water-spray mixtures only. Do not use 2,4-D sprays if orchard usually pro- duces rough fruit, or if an early harvest is planned. See tables 1 and 2 for further data. PREPARATION OF 2,4-D SPRAYS The amount of 2,4-D needed in a spray is based on the pounds per gallon of 2,4-D acid equivalent contained in the commer- cial preparation. This acid equivalent is stated on the label of the container. To find ppm concentration of 2,4-D for volumes other than 500 gallons, use the following formula: Parts per million x gallons in hundreds x factor (see table 2) = amount of 2,4-D in ml (milliliters). Example 7. To get 12 ppm in 400 gallons of spray mixture when using a 4 pounds per gallon material. Use 1 ppm per 100 gallon value 0.79. Multiply 12 ppm x 4 (gallons in hundreds) x 0.79 = 38 ml( 37.9 rounded) of 2,4-D. Example 2. To get 4 ppm in 600 gallons of spray mixture when using a 3.44 pounds per gallon material. Use 1 ppm per 100 gallon value 0.95. Multiply 4 ppmx6 (gallons in hundreds) x 0.95 = 23 ml (22.8 rounded) of 2,4-D. Table 2. Common Acid-equivalent Values of 2,4-D and Amounts of 2,4-D in Milliliters Used to Obtain Various Parts Per Million Concentration of 2,4-D in 500 Gallons of Spray Concentrations of 2,4-D Factor in milli- liters for 1 part Acid equivalent 4 ppm 8 ppm 16 ppm 20 ppm 24 ppm per million per 100 gallons 4 pounds per gallon 3.34 pounds per gallon 3 pounds per gallon 2 pounds per gallon 1 pound per gallon 15.8 ml 18.9 21.1 31.6 63.1 31.6 ml 37.8 42.1 63.2 126.3 63.2 ml 75.6 84.2 126.3 252.6 79.0 ml 94.6 105.3 157.9 315.8 94.7 ml 113.5 126.3 189.5 379.0 0.7896 0.9456 1.0528 1.579 3.1586 10 HOW THE UNIVERSITY OF CALIFORNIA WORKS WITH AGRICULTURE As one of the nation's Land-Grant institutions, the University of California plays a multiple role in service to agriculture. This involves teaching, re- search, and conveying the facts developed by research to those who may put them to good use in the best interest of all the people. These activities are combined in the University's Division of Agricultural Sciences. This statewide framework includes: The College of Agriculture providing instruction in agriculture and re- lated sciences on campuses at Berkeley, Davis, Los Angeles, and Riverside. The Schools of Forestry and Veterinary Medicine function as separate pro- fessional schools within the Division but are closely related to the College of Agriculture. The Agricultural Experiment Station conducting research on the four campuses mentioned above as well as on numerous field stations, experi- mental areas, and farms throughout the state. Closely allied with the Ex- periment Station are the Gianni ni Foundation of Agricultural Economics and the Kearny Foundation of Soil Science. The Agricultural Extension Service with 53 offices serving 56 counties carrying out the responsibility of "extending" research results to the people. The service cooperates with the Experiment Station in local research on thousands of farms. It also conducts youth educational activities through the 4-H Club program. Co-operative Extension work in Agriculture and Home Economics, College of Agriculture, University of California, and United States Department of Agriculture cooperating. Distributed in furtherance of the Acts of Congress of May 8, and June 30, 1914. George B. Alcorn, Director, California Agricultural Extension Service. 15m-5,'64(E4120)V.L. w 3 *-#-* BLACK BOXES... Agriculture has them too Both manned and unmanned vehicles sent into space are equipped with "black boxes" that record or transmit in- formation needed by space scientists who hope to explore other planets. Not as glamorous perhaps, but equally important to our country's welfare are the measuring devices used by agri- cultural scientists to gain knowledge that will improve conditions on our own planet. From information in such "black boxes" will come better farming methods, better foods and fibers, better living. The agricultural sciences offer rewarding careers for qualified young men and women who would have a part in making the future better. Write for the booklet AGRI- CULTURE-OPEN DOOR TO YOUR FUTURE. -w* *' vj) Agricultural Publications University Hall University of California Berkeley 4, Calif. fc **^v$