UNIVERSITY OF CALIFORNIA 
 
 COLLEGE OF AGRICULTURE 
 
 AGRICULTURAL EXPERIMENT STATION 
 
 BERKELEY, CALIFORNIA 
 
 CIRCULAR 322 
 
 August, 1931 
 
 AN INFECTIOUS BRAIN DISEASE OF 
 HORSES AND MULES 
 
 {Encephalomyelitis ) 
 C. M. HARING,i J. A. HOWARTH, 2 and K. F. MEYER3 
 
 A rapidly spreading" disease of horses and mules prevailed in the 
 San Joaquin Valley of California from July to November, 1930 
 (fig. 1A), and reappeared in several widely separated parts of the 
 state in July, 1931 (fig. IB). The malady is apparently identical with 
 the horse disease which at different times during the past sixty years 
 has caused heavy losses in various parts of the United States, particu- 
 larly in the west central states, and has been called ' ' Kansas-Nebraska 
 horse plague," and also, incorrectly, "cerebrospinal meningitis,' ' 
 ft nonpurulent encephalitis, " "forage poisoning" and "botulism." 4 
 
 Studies by Meyer, Haring, and Howitt 5 at the University of Cali- 
 fornia have shown that the present epizootic in California horses 
 is caused by a filter-passing virus which produces an inflammation 
 in the brain (encephalitis) and also in the spinal cord (myelitis). 
 Accordingly, the suitable medical name for the disease should be 
 encephalomyelitis. 6 
 
 i Professor of Veterinary Science and Veterinarian in the Experiment Station. 
 
 2 Associate in Veterinary Science. 
 
 3 Professor of Bacteriology, Director of the Hooper Foundation, and Director 
 of Research in the National Canners' Association Laboratory. 
 
 4 Botulism is a poisoning caused by eating spoiled material in which toxins 
 have been formed by the growth of various types of Clostridium botulinus. The 
 symptoms in horses somewhat resemble encephalomyelitis but otherwise the 
 diseases are not related. 
 
 s Meyer, K. F., O. M. Haring, and B. Howitt. Newer knowledge of the 
 neurotropic virus infections of the horse. Jour. Amer. Vet. Med. Assoc. In 
 press. 
 
 6 In Germany a similar disease has been known for many years, and is called 
 "Borna disease" after a town in Saxony. This disease of horses in Germany 
 is also caused by a filterable virus which produces an inflammation in the 
 central nervous system, but which is probably not identical with the American 
 virus. 
 
University of California — Experiment Station 
 
 d £S 
 * ftg 
 
 °^§ 
 
 o 
 
 XI BO 
 O 53 
 O 
 
 £H 
 
 53 £ 
 
 as 
 
 53 53 o 
 .2 £ * 
 
 OH o ci 
 
 ^3 £ 
 
 •D O ~ 
 
 p 4) l? 
 t> cj 53 
 § § O 
 
 Oo 
 
 rt o ft 
 -92 d 
 
 ft 
 -d ft 
 
 ►T <v 
 
 d 
 
 ~ o w 
 
 *" ft 03 
 
 pC Jh-P 
 
 
Cir. 322] An Infectious Brain Disease of Horses and Mules 3 
 
 The following statements regarding epizootic encephalomyelitis in 
 horses and mules represent the present concensus of opinion of Cali- 
 fornia veterinarians who have had experience with the disease. These 
 
 * J Mi . .# , 
 
 Fig. 2. — Sleepy type. 
 
 Fig. 3. — Walking type. 
 
 recommendations have been prepared for emergency publication to 
 furnish immediately-needed information to horse owners. It is antici- 
 pated that in a few months more information will be available. 
 
University of California — Experiment Station 
 
 CHARACTERISTICS OF THE DISEASE 
 
 Animals Affected. — Horses and mules are the only animals that 
 have been observed to contract the disease under farm conditions. 7 
 Age and sex do not seem to be limiting factors. Stallions, as well as 
 geldings and mares, and suckling colts, as well as aged horses, are 
 susceptible. No cases have been reported in this state in animals other 
 than those kept on farms. The disease thus far has been confined to 
 those sections where the land is more or less cultivated, and to date no 
 losses have been reported to the writers in range animals outside of 
 irrigated or farming districts or in horses kept in city stables, such as 
 riding academies, except in certain cases recently brought in from 
 the country. 
 
 Symptoms. — In severe cases the animals fall down and are unable 
 to get up or roll over. They lie on their sides, convulsively paw the 
 air and ground and bang their heads against the ground, causing 
 severe bruising especially over the eye. In advanced stages the horses 
 are relatively quiet. 
 
 Inability to swallow, spasms of the muscles of the face, neck (fig. 9) , 
 and limbs severe grinding of the teeth, a bubbling noise in the throat 
 and very foul-smelling breath are characteristic of advanced, acute 
 cases. Such animals if unable to rise are hopeless and should be 
 destroyed for humane reasons. 
 
 In milder cases the animals stagger, sway from front to rear while 
 standing, tend to lean against supporting objects, and frequently 
 grind their teeth or yawn (fig. 8). Two quite different types are 
 common : the sleepy type, which drowses until disturbed, when it fre- 
 quently becomes convulsive ; and the walking type, which continues to 
 pace round and round (figs. 2 and 3). If in a field, such cases usually 
 follow close to the fence and only change direction when they come in 
 contact with an obstacle or a turn in the fence. When undisturbed, 
 some stand with the head against a wall or fence (figs. 4 and 5). 
 
 Recognition of the first symptoms is of importance because the 
 chances of recovery are greater in animals that are carefully treated 
 and nursed from the very beginning. Unwillingness to be led, lack 
 
 7 Experiments at the University of California have shown that the infective 
 virus which is present in the central nervous system of the sick horses can 
 produce the disease in horses, monkeys, rabbits, guinea pigs, rats, and mice 
 when it is injected directly into the brain, and in some instances when dropped 
 into the nostrils or injected subcutaneously. 
 
Or. 322] An Infectious Brain Disease of Horses and Mules 5 
 
 of spirit, slightly wobbly gait, failure to come when called, or, in 
 unbroken colts, failure to run when approached, are indications. In 
 some animals the first symptoms are a peculiar tightness of the lips ; 
 in other cases, the lips are abnormally loose, or twisted to one side 
 
 Fig. 4. — Stance when undisturbed, case B-24. 
 
 Fig. 5. — Stance when disturbed, case B-24. 
 
 (figs. 6 and 7). In advanced stages of the disease, the temperature is 
 usually normal unless complications, such as pneumonia, have set in, 
 but in the early stages the temperature may range from 99° Fahren- 
 heit (normal) to 107° F, according to the individual case. 
 
6 University of California — Experiment Station 
 
 The Spread of the Disease. — The disease spread rapidly during- the 
 months of July and August, 1930, when it invaded all of the horse- 
 raising areas in the San Joaquin Valley. During the following three 
 months, the spread to new areas ceased, but heavy losses continued in 
 the sections already affected. With the advent of cool weather in 
 
 Fig. 6. — A, Tightening- of the lips and dilation of the nostrils often continues 
 through the course of the disease, as in this case. 
 
 B, Looseness of the lips may be one of the first symptoms, as in this case. 
 
 A B 
 
 Fig. 7. — A, Spasm of the facial muscles may cause wrinkling of the muzzle, 
 as in this case. 
 
 B, Muzzle twisted toward the right, caused by left facial paralysis. 
 
 October and November, the disease disappeared. During the following 
 winter and spring, only occasional cases were reported, but with the 
 onset of very hot weather, late in June, 1931, the disease reappeared 
 in the San Joaquin Valley, and heavy losses have also been reported 
 from the Sacramento Valley and in Ventura County. 
 
 Little knowledge is available to indicate how the disease is intro- 
 duced into a community, how it spreads from farm to farm or from 
 
Cir. 322] An Infectious Brain Disease of Horses and Mules 7 
 
 animal to animal on the same farm. The role that flies and other 
 biting" insects may or may not have in transmitting the disease is 
 unknown. It is the general belief that the infection is carried by food 
 or water which has at some time been contaminated by infected animals. 
 Morbidity. — About one horse in ten in the San Joaquin Valley 
 showed symptoms in 1930. Approximately 6,000 head developed the 
 
 Fig. 8. — Yawning is a frequent symptom. 
 
 Fig. 9. — Spasm of the neck muscles twists the head to one side in this stallion. 
 
 disease, and about 3,000 of these cases terminated fatally. On one 
 ranch with 687 horses and mules, 67 contracted encephalomyelitis and 
 32 died. On certain farms higher proportions than this have occurred, 
 but it is relatively rare for more than one of a team to show symptoms. 
 This peculiarity can be explained by the hypothesis that many horses 
 contract the disease in such a mild form that it is not recognized. 
 
University of California — Experiment Station 
 
 RECOMMENDATIONS TO ALL HORSE OWNERS IN INFECTED AREAS 
 
 The most hopeful preventive measures consist in the complete 
 isolation of all horses so far as is practicable. It behooves one to 
 take into consideration every possible means by which the disease may 
 be carried from an infected farm to premises where no cases have 
 existed ; therefore : 
 
 Prevent the association of horses from infected areas with those 
 
 from noninfected areas. 
 Do not use water from troughs, 1^1^^^ ditches, buckets, etc., 
 
 which have been used by horses on or from infected premises. 
 Keep horses away from streams, canals, ditches, and pools. Provide 
 
 an abundant supply of fresh well water. 
 
 Suspend breeding operations where horses must be moved from 
 farm to farm. There is a possibility of carrying the disease by moving 
 animals or materials from one farm to another. Judging by what 
 occurs in similar but better-known diseases, one is justified in suspect- 
 ing that apparently normal horses which have been exposed may be 
 carriers of the virus. 
 
 Protect the animals as much as practicable from flies and other 
 biting insects. In the case of certain very valuable animals, it may 
 be worth while, during very hot weather, to confine them in darkened 
 stalls during the day time and use fly sprays liberally. The fact that 
 few cases have been reported in riding academy horses, polo ponies, 
 or city dray horses would justify the precaution of temporarily bring- 
 ing into barns the very valuable animals now at pasture. 
 
 A veterinarian in one of the worst-infected sections of California 
 writes that all cases of encephalomyelitis in his practice had been fed 
 on the ground. He advised feeding from a rack of some kind and 
 killing rats, mice, and ground squirrels. He suggested that efforts 
 be made to stop the transportation of manure from infected districts 
 to localities where the disease has not as yet been diagnosed. 
 
Cm. 322] An Infectious Brain Disease of Horses and Mules 
 
 RECOMMENDATIONS TO OWNERS OF SICK HORSES 
 
 Obt&in the services of a \eterinarian as soon as possible. Persons 
 not accustomed to seeing and working with this malady might mistake 
 it in the early stages for azoturia, colic, poisoning, heat stroke, or 
 "staggers" from various other causes. 
 
 Keep the sick animals quiet and in the shade. It is imperative that 
 the animals be kept as quiet as possible. Regardless of how slight the 
 manifestation of the disease may be, do not work or exercise an 
 affected animal, as any unnecessary muscular exertion will aggravate 
 the condition. Should the horse become affected at a great distance 
 from the barn, it should not be forced to make the trip back — an 
 
 Fig. 10. — A good type of supporting frame with a strong rear crossbar and 
 a feed box high enough to keep the head level with the body. 
 
 improvised shade should be erected. Sunlight seems to be of no value 
 in hastening recovery. Horses and mules allowed to remain in the 
 direct sunlight during hot weather will only succumb more rapidly. 
 
 Protection from Injury. — Care should be exercised to prevent a 
 sick animal from injuring itself. This may be accomplished by plac- 
 ing the horse or mule between two timbers held fast by four posts 
 with wooden braces across each end (see fig. 10). Cross timbers, front 
 and rear, are necessary because the animal rests by sitting back or 
 leaning forward. Sacks or padding tied to the timbers will prevent 
 undue injury to the body. A box should be placed at one end to hold 
 a water bucket, and a rack should be provided for hay. Chances for 
 recovery are better if the animal can be kept standing. This may be 
 accomplished by the use of canvas or wide belting passed under the 
 abdomen and chest and attached to the side bars. 
 
10 
 
 University of California — Experiment Station 
 
 Support by an abdominal sling, as shown in figure 11, may be 
 preferable in very severe cases. This should be used in conjunction 
 with the side and cross bars, and not as shown in figure 12, so that 
 
 Fig. 11. — The combined use of a sling and supporting frame is desirable 
 
 in severe cases. 
 
 Fig. 12. — -The use of a sling without any supporting frame is valueless in 
 severe cases such as this. 
 
 during periods of collapse support will be furnished for the head 
 and rump. 
 
 Some veterinarians prefer to omit the supporting devices and place 
 the animal in a large box stall. It should be very well bedded with 
 
Cm. 322] An Infectious Brain Disease of Horses and Mules 
 
 11 
 
 straw, however, and if the animal gets down an effort should be made 
 to keep it in the breast position shown in figure 14. Bales of hay and 
 sacks stuffed with straw are useful in doing this. If left- to lie flat on 
 
 >*Asp*£*-'-Wr 
 
 Fig. 13. — Animal lying flat on its side without any protection from the 
 ground. Do not permit this when possible to get it to stand or assume a breast 
 position. 
 
 Fig. 14. — Assisting an animal to assume a breast position. 
 
 its side, the body and head soon become seriously bruised from con- 
 tact with the ground (fig. 13). Two or three times a day the animal 
 should be assisted to its feet, A veterinarian who treated about 75 
 
12 University of California — Experiment Station 
 
 cases during" July, 1931, writes: "The horses that were able to get up 
 on their feet occasionally during the first four days of the sickness 
 all got well." 
 
 Animals that are unable to rise should be turned over at four or 
 five-hour intervals to avoid congestion of the blood in the lower part 
 of the body. This will also tend to prevent "bedsores," pneumonia, 
 and other complications which quickly develop if a horse lies for a 
 long time on one side. 
 
 Feed, — Small amounts of wholesome food, such as freshly-cut 
 alfalfa or cured alfalfa, or, if neither is available, good grain hay, will 
 materially help in sustaining the strength of the affected horse. In 
 mild cases, the appetite usually remains normal and many horses badly 
 affected will consume some food if it is placed before them. They 
 often nibble hay from the rack or ground as the disease progresses. 
 Feeding will take place only when the sick animal can secure food with 
 little or no effort. Some of the animals refuse food for a few days, but, 
 as recovery sets in, the appetite returns to normal and should be 
 satisfied with the best quality of feed obtainable. 
 
 Water. — Thirst is one of the most common symptoms, and it is 
 generally believed by veterinarians that the animals should be per- 
 mitted to drink as often and as much as they wish. 
 
 Studies at the University of California have shown that, in 
 advanced cases, there is a marked dehydration of the blood and tissues. 
 This indicates that sick horses should be induced to drink as frequently 
 as practicable. Those that are able to swallow usually drink eagerly 
 and should have constant access to a good, clean water supply. They 
 should not be made to drink from containers on the ground as they 
 will have difficulty in keeping their balance while lowering the head. 
 In some cases, there is a semiparalysis of the lips, tongue, and pharyn- 
 geal muscles. Such animals will often dip the muzzle in water but 
 apparently make no attempt to drink. If water is slowly injected into 
 the mouth (fig. 15), they generally swallow willingly. Time and 
 patience will be necessary in cases that have difficulty in drinking. 
 Care should be taken not to raise the mouth and nose too high nor to 
 attempt to force water or other liquids down the throat. 
 
 Avoid drenching. Attempts by forced drenching to administer oils, 
 salts, and other drugs to animals affected with encephalomyelitis are 
 very apt to result disastrously. The horse may be in a state of stupor 
 or collapse, or it may be excited. Either of these conditions is asso- 
 ciated with difficulty in swallowing. Elevating the head, as is usually 
 done in drenching, may cause the fluid to pass down the trachea or 
 
Cm. 322] An Infectious Brain Disease of Horses and Mules 13 
 
 windpipe into the lungs, which, in turn, in these cases almost invariably 
 produces a mechanical pneumonia. 
 
 The safest way to introduce liquids into the stomach of a horse or 
 mule is by the use of a stomach tube. On account of the difficulty of 
 passing the tube through the mouth of these animals, it is usually 
 passed via a nostril. This operation should be done by a veterinarian. 
 In certain cases the use of the stomach tube has been found to be 
 injurious because its passage has induced pneumonia from inhalation 
 into the lungs of putrid saliva collected in the throat. Rectal injec- 
 
 . „ - 
 
 > ^^V^'N* 
 
 Fig. 15. — Assisting a thirsty animal to drink. A short piece of metal pipe is 
 inserted a few inches into the mouth. To prevent choking do not insert the 
 pipe too far nor raise the head too high. 
 
 tions may be helpful but should be given only on a veterinarian's 
 advice. 
 
 Do not experiment with drugs not prescribed or recommended by 
 a veterinarian. The chances of recovery will probably be lessened by 
 such dosing. Even if the drugs should be harmless, the unnecessary 
 disturbance and irritation in giving them to the animal may reduce 
 the chance of recovery. The sick animal should be kept as quiet and 
 undisturbed as possible. 
 
 Each case requires special, individual treatment. No specific treat- 
 ment generally applicable to all cases has yet been found. The most 
 successful veterinarians depend on conservative efforts to alleviate the 
 symptoms. University veterinarians, in cooperation with various 
 
14 University of California — Experiment Station 
 
 veterinary practitioners, have tried a number of chemicals and serums, 
 but as yet nothing' has been found of special value in neutralizing or 
 reducing the effects of the virus which is the primary cause of the 
 disease. 
 
 Experiments with the virus under controlled conditions are being 
 actively carried on at the University of California. Any results of 
 practical value will be immediately made public. 
 
STATION PUBLICATIONS AVAILABLE FOR FREE DISTRIBUTION 
 
 BULLETINS 
 
 No. 
 
 253. Irrigation and Soil Conditions in the 
 
 Sierra Nevada Foothills, California. 
 263. Size Grades for Ripe Olives. 
 277. Sudan Grass. 
 279. Irrigation of Rice in California. 
 283. The Olive Insects of California. 
 304. A Study of the Effects of Freezes on 
 
 Citrus in California. 
 310. Plum Pollination. 
 331. Phylloxera-resistant Stocks. 
 335. Cocoanut Meal as a Feed for Dairy 
 
 Cows and Other Livestock. 
 
 343. Cheese Pests and Their Control. 
 
 344. Cold Storage as an Aid to the Market- 
 
 ing of Plums, a Progress Report. 
 
 347. The Control of Red Spiders in Decid- 
 
 uous Orchards. 
 
 348. Pruning Young Olive Trees. 
 
 349. A Study of Sidedraft and Tractor 
 
 Hitches. 
 357. A Self-Mixing Dusting Machine for 
 Applying Dry Insecticides and Fun- 
 gicides. 
 
 361. Preliminary Yield Tables for Second- 
 
 Growth Redwood. 
 
 362. Dust and the Tractor Engine. 
 
 363. The Pruning of Citrus Trees in Cali- 
 
 fornia. 
 
 364. Fungicidal Dusts for the Control of 
 
 Bunt. 
 366. Turkish Tobacco Culture, Curing, and 
 Marketing. 
 
 368. Bacterial Decomposition of Olives 
 
 During Pickling. 
 
 369. Comparison of Woods for Butter Boxes. 
 
 370. Factors Influencing the Development 
 
 of Internal Browning of the Yellow 
 Newtown Apple. 
 
 371. The Relative Cost of Yarding Small 
 
 and Large Timber. 
 
 373. Pear Pollination. 
 
 374. A Survey of Orchard Practices 
 
 Citrus Industry of Southern 
 
 fornia. 
 379. Walnut Culture in California. 
 386. Pruning Bearing Deciduous 
 
 Trees. 
 
 388. The Principles and Practice of 
 
 Drying Fruit. 
 
 389. Berseem or Egyptian Clover. 
 
 390. Harvesting and Packing Grapes in 
 
 California. 
 
 391. Machines for Coating Seed Wheat with 
 
 Copper Carbonate Dust. 
 
 392. Fruit Juice Concentrates. 
 
 393. Crop Sequences at Davis. 
 
 394. I. Cereal Hay Production in California. 
 
 II. Feeding Trials with Cereal Hays. 
 396. The Mat Bean, Phaseolus Aconitifolius. 
 
 404. The Dehydration of Prunes. 
 
 405. Citrus Culture in Central California. 
 
 406. Stationary Spray Plants in California. 
 
 407. Yield, Stand, and Volume Tables for 
 
 White Fir in the California Pine 
 Region. 
 
 408. Alternaria Rot of Lemons. 
 
 409. The Digestibility of Certain Fruit By- 
 
 products as Determined for Rumi- 
 nants. Part I. Dried Orange Pulp 
 and Raisin Pulp. 
 
 410. Factors Influencing the Quality of Fresh 
 
 Asparagus After it is Harvested. 
 
 412. A Study of the Relative Value of Cer- 
 tain Root Crops and Salmon Oil as 
 Sources of Vitamin A for Poultry. 
 
 414. Planting and Thinning Distances for 
 Deciduous Fruit Trees. 
 
 in the 
 Cali- 
 
 Fruit 
 Sun- 
 
 No. 
 
 415. 
 
 416. 
 
 418. 
 
 419. 
 
 420. 
 
 421. 
 423. 
 
 425. 
 426. 
 427. 
 
 428. 
 
 431. 
 432. 
 433. 
 
 434. 
 435. 
 
 436. 
 438. 
 439. 
 
 440. 
 
 444. 
 
 445. 
 
 446. 
 447. 
 
 448. 
 
 449. 
 
 450. 
 
 451. 
 
 452. 
 454. 
 
 455. 
 
 456. 
 
 458. 
 
 459. 
 
 460. 
 
 462. 
 464, 
 
 465. 
 
 The Tractor on California Farms. 
 
 Culture of the Oriental Persimmon in 
 California. 
 
 A Study of Various Rations for Fin- 
 ishing Range Calves as Baby Beeves. 
 
 Economic Aspects of the Cantaloupe 
 Industry. 
 
 Rice and Rice By-Products as Feeds 
 for Fattening Swine. 
 
 Beef Cattle Feeding Trials, 1921-24. 
 
 Apricots (Series on California Crops 
 and Prices). 
 
 Apple Growing in California. 
 
 Apple Pollination Studies in California. 
 
 The Value of Orange Pulp for Milk 
 Production. 
 
 The Relation of Maturity of California 
 Plums to Shipping and Dessert 
 Quality. 
 
 Raisin By-Products and Bean Screen- 
 ings as Feeds for Fattening Lambs. 
 
 Some Economic Problems Involved in 
 the Pooling of Fruit. 
 
 Power Requirements of Electrically 
 Driven Dairy Manufacturing Equip- 
 ment. 
 
 Investigations on the Use of Fruits in 
 Ice Cream and Ices. 
 
 The Problem of Securing Closer Rela- 
 tionship between Agricultural Devel- 
 opment and Irrigation Construction. 
 
 I. The Kadota Fig. II. The Kadota 
 Fig Products. 
 
 Grafting Affinities with Special Refer- 
 ence to Plums. 
 
 The Digestibility of Certain Fruit By- 
 Products as Determined for Rumi- 
 nants. Part II. Dried Pineapple 
 Pulp, Dried Lemon Pulp, and Dried 
 Olive Pulp. 
 
 The Feeding Value of Raisins and 
 Dairy By-Produces for Growing and 
 Fattening Swine. 
 
 Beans (Series on California Crops and 
 Prices). 
 
 Economic Aspects of the Apple In- 
 dustry. 
 
 The Asparagus Industry in California. 
 
 A Method of Determining the Clean 
 Weights of Individual Fleeces of Wool. 
 
 Farmers' Purchase Agreement for Deep 
 Well Pumps. 
 
 Economic Aspects of the Watermelon 
 Industry. 
 
 Irrigation Investigations with Field 
 Crops at Davis, and at Delhi, Cali- 
 fornia, 1909-1925. 
 
 Studies Preliminary to the Establish- 
 ment of a Series of Fertilizer Trials 
 in a Bearing Citrus Grove. 
 
 Economic Aspects of the Pear Industry. 
 
 Rice Experiments in Sacramento Val- 
 ley, 1922-1927. 
 
 Reclamation of the Fresno Type of 
 Black-Alkali Soil. 
 
 Yield, Stand and Volume Tables for 
 Red Fir in California. 
 
 Factors Influencing Percentage Calf 
 Crop in Range Herds. 
 
 Economic Aspects of the Fresh Plum 
 Industry. 
 
 Lemons (Series on California Crops 
 and Prices). 
 
 Prune Supply and Price Situation. 
 
 Drainage in the Sacramento Valley 
 Rice Fields. 
 
 Curly Top Symptoms of the Sugar Beet. 
 
BULLETINS— (Continued) 
 
 No. No, 
 
 466. The Continuous Can Washer for Dairy 484. 
 
 Plants. 
 
 467. Oat Varieties in California. 485. 
 
 468. Sterilization of Dairy Utensils with 486. 
 
 Humidified Hot Air. 
 
 469. The Solar Heater. 487. 
 
 470. Maturity Standards for Harvesting 
 
 Bartlett Pears for Eastern Shipment. 488. 
 
 471. The Use of Sulfur Dioxide in Shipping 
 
 Grapes. 489. 
 
 472. Adobe Construction. 
 
 473. Economic Aspects of the Sheep In- 
 
 dustry. 
 
 474. Factors Affecting the Cost of Tractor 490. 
 
 Logging in the California Pine 
 
 Region. 491. 
 
 475. Walnut Supply and Price Situation. 
 
 477. Improved Methods of Harvesting Grain 492. 
 
 Sorghum. 493. 
 
 478. Feeding and Management of Dairy 494. 
 
 Calves in California. 495. 
 
 479. I. Irrigation Experiments with Peaches 
 
 in California. II. Canning Quality 496. 
 
 of Irrigated Peaches. 
 
 480. The Use, Value, and Cost of Credit in 
 
 Agriculture. 497. 
 
 481. Utilization of Wild Oat Hay for Fat- 
 
 tening Yearling Steers. 498. 
 
 482. Substitutes for Wooden Breakpins. 500. 
 
 483. Utilization of Surplus Prunes. 
 
 The Effects of Desiccating Winds on 
 
 Citrus Trees. 
 Drying Cut Fruits. 
 Pullorum Disease (Bacillary White 
 
 Diarrhea of Chickens). 
 Asparagus (Series on California Crops 
 
 and Prices). 
 Cherries (Series on California Crops 
 
 and Prices). 
 Irrigation Water Requirement St\idies 
 
 of Citrus and Avocado Trees in San 
 
 Diego County, California, 1926 and 
 
 1927. 
 Olive Thinning and Other Means of 
 
 Increasing Size of Olives. 
 Yield, Stand, and Volume Tables for 
 
 Douglas Fir in California. 
 Berry Thinning of Grapes. 
 Fruit Markets in Eastern Asia. 
 Infectious Bronchitis in Fowls. 
 Milk Cooling on California Dairy 
 
 Farms. 
 Precooling of Fresh Fruits and Tem- 
 peratures of Refrigerator Cars and 
 
 Warehouse Rooms. 
 A Study of the Shipment of Fresh 
 
 Fruits and Vegetables to the Far East. 
 Pickling Green Olives. 
 Dehydration of Grapes. 
 
 No. 
 
 117. The Selection and Cost of a Small 
 
 Pumping Plant. 
 127. House Fumigation. 
 178. The Packing of Apples in California. 
 203. Peat as a Manure Substitute. 
 212. Salvaging Rain-Damaged Prunes. 
 230. Testing Milk, Cream, and Skim Milk 
 
 for Butterfat. 
 232. Harvesting and Handling California 
 
 Cherries for Eastern Shipment. 
 
 239. Harvesting and Handling Apricots and 
 
 Plums for Eastern Shipment. 
 
 240. Harvesting and Handling California 
 
 Pears for Eastern Shipment. 
 
 241. Harvesting and Handling California 
 
 Peaches for Eastern Shipment. 
 
 243. Marmalade Juice and Jelly Juice from 
 
 Citrus Fruits. 
 
 244. Central Wire Bracing for Fruit Trees. 
 
 245. Vine Pruning Systems. 
 
 248. Some Common Errors in Vine Pruning 
 
 and Their Remedies. 
 
 249. Replacing Missing Vines. 
 
 250. Measurement of Irrigation Water on 
 
 the Farm. 
 
 253. Vineyard Plans. 
 
 255. Leguminous Plants as Organic Ferti- 
 lizers in California Agriculture. 
 
 257. The Small-Seeded Horse Bean (Vicia 
 
 faba var. minor). 
 
 258. Thinning Deciduous Fruits. 
 
 259. Pear By-Products. 
 
 261. Sewing Grain Sacks. 
 
 262. Cabbage Production in California. 
 
 263. Tomato Production in California. 
 
 265. Plant Disease and Pest Control. 
 
 266. Analyzing the Citrus Orchard by Means 
 
 of Simple Tree Records. 
 
 CIRCULARS 
 
 No. 
 
 269. 
 
 270. 
 
 273. 
 
 276. 
 
 278. 
 
 279. 
 
 282. 
 
 287. 
 288. 
 290. 
 294. 
 295. 
 296. 
 
 301. 
 302. 
 304. 
 307. 
 308. 
 310. 
 
 311. 
 
 312. 
 
 313. 
 316. 
 
 317. 
 
 318. 
 319. 
 320. 
 
 An Orchard Brush Burner. 
 
 A Farm Septic Tank. 
 
 Saving the Gophered Citrus Tree. 
 
 Home Canning. 
 
 Olive Pickling in Mediterranean 
 
 Countries. 
 The Preparation and Refining of Olive 
 
 Oil in Southern Europe. 
 Prevention of Insect Attack on Stored 
 
 Grain. 
 Potato Production in California. 
 Phylloxera Resistant Vineyards. 
 The Tangier Pea. 
 Propagation of Deciduous Fruits. 
 Growing Head Lettuce in California. 
 Control of the California Ground 
 
 Squirrel. 
 Buckeye Poisoning of the Honey Bee. 
 The Sugar Beet in California. 
 Drainage on the Farm. 
 American Foulbrood and Its Control. 
 Cantaloupe Production in California. 
 The Operation of the Bacteriological 
 
 Laboratory for Dairy Plants. 
 The Improvement of Quality in Figs. 
 Principles Governing the Choice, Oper- 
 ation, and Care of Small Irrigation 
 
 Pumping Plants. 
 Fruit Juices and Fruit Juice Beverages. 
 Electrical Statistics for California 
 
 Farms. 
 Fertilizer Problems and Analysis of 
 
 Soils in California. 
 Termites and Termite Damage. 
 Pasteurizing* Milk for Calf Feeding. 
 Preservation of Fruits and Vegetables 
 
 by Freezing Storage. 
 
 16m-8,'31