CIHM 
 Microfiche 
 Series 
 (Monographs) 
 
 ICMH 
 
 Collection de 
 microfiches 
 (monographies) 
 
 Canadian Institute for Historical Microroproductions / Institut canadien de microreproducbons historiques 
 
 ^iogo 
 
Technical and Bibli' .: ..phi:; Notes / Notes techniques et bibliographiques 
 
 The Institute ' ^s attempted to or. cX, Va c^st original 
 copy available ior filming. Feat; , ... cf th:c oopy whi„h 
 may be bibliographically unique, vvri.^ ray a;ter any of 
 the images in the reproduction, or A^^ich may 
 significantly change the usual method of timing are 
 checked below. 
 
 n 
 
 n 
 □ 
 
 Coloured covers / 
 Couverture de couleur 
 
 Covers damaged / 
 Couverture endommagee 
 
 Covers restored and/or laminated / 
 Couverture restauree et/ou pelliculee 
 
 Cover title missing / Le titre de couverture manque 
 
 Coloured maps / Carles geographiques en couleur 
 
 Coloured ink (i.e. other than blue or black) / 
 Encre de cculeur (i.e. autre que bleue ou noire) 
 
 Coloured plates and/or illustranons / 
 Planches el^ou illustrations en couleur 
 
 Bound with other material / 
 Relie avec d'autres Documents 
 
 Only edition available / 
 Seule edition disponible 
 
 Tight binding may cause shadow? or distortion along 
 interior margin / La reliure serree peut causer de 
 I'ombre ou de la distorsion le long de la marge 
 inteneure. 
 
 Blank leaves added during restorations may appear 
 within the text. Whenever possible, these have been 
 omitted from filming / II se peut que certaines pages 
 blanches ajoutees lors d'une restauration 
 apparaissent dans le te.xte, mais, lorsque cela e'ait 
 possible, ces pages n'ont pas ete filmees. 
 
 L'Institut a microfilme le meilleur exemplaire qu'il lui a 
 ete possible de se procurer. Les details de cet exem- 
 plaire qui sont peut-etre uniques du point de vue bibli- 
 ographique, qui peuvent modifier une image reproduite, 
 ou qui peuvent exiger une modification dans la metho- 
 de normale de filmage sont indiques ci-dessous. 
 
 I i Coloured pages / Pages de couleur 
 
 I I Pages damaged / Pages endommagees 
 
 n 
 
 Pages restored and/or laminated / 
 Pages restaurees et/ou pelliculees 
 
 D 
 
 □ Pages discoloured, stained or foxed / 
 Pages decolorees, tachetees ou piquees 
 
 Pages detached / Pages detachees 
 
 Showlhrough / Transparence 
 
 Quality of print varies / 
 Qualite inegale de I'impression 
 
 Includes supplementary material / 
 Comprend du materiel supplementaire 
 
 Pages wholly or partially obscured by errata slips, 
 tissues, etc., have been refilmed to ensure the best 
 possible image / Les pages totalement ou 
 partiellement obscurcies par un feuillet d'errata, une 
 peiure, etc., ont ete filmees a nouveau de fa^on a 
 obtenir la meilieure image possible. 
 
 Opposing pages with varying colouration or 
 discolourations are filmed twice to ensure the best 
 possible image / Les pages s'opposant ayant des 
 colorations variables ou des decolorations sont 
 filmees deux fois afin d'obtenir la meilieure image 
 possible. 
 
 n 
 
 □ Additional comments / 
 Commentaires supplemenlaires: 
 
 Pagination is as follons: p. 239-29b. 
 
 This Item IS filmed at the reduction ratio checked below / 
 
 Ce document est filme au t.iuK de reduction indiquo cidessous. 
 
 10x 
 
 
 
 
 14x 
 
 
 
 
 18x 
 
 
 
 22x 
 
 
 
 
 26x 
 
 
 
 
 30x 
 
 
 
 
 
 
 
 
 
 
 
 
 i 
 
 
 
 
 
 
 
 
 
 
 
 
 
 12x 
 
 16x 
 
 20x 
 
 24x 
 
 28x 
 
 32x 
 
The copy filmed here has been reproduced thanks 
 to the generosity of: 
 
 J.J. Talfivan Regional Collection, 
 D.B. Weldon Library, 
 University of Western Ontario 
 
 The images appearing here are the best quality 
 possible considering the condition and legibiliry 
 of the original copy and in keeping with the 
 filming contract specifications. 
 
 Original copies in printed paper covers are filmed 
 beginning with the front cover and ending on 
 the last page with a printed or illustrated impre«- 
 sion, or the back cover when appropriate. All 
 other original copies are filmed beginning on the 
 first page with a printed or illustrated impres- 
 sion, and ending on the last page with a printed 
 or illustrated impression. 
 
 The last recorded frame on each microfiche 
 shall contain the symbol — ^ (meaning "CON- 
 TINUED"), or the symbol V (meaning "END"). 
 whichever applies. 
 
 L'exemplaire film* fut reproduit grace A la 
 g^n^rosit^ de: 
 
 J.J. Talman Regional Collection, 
 D.B. Weldon Library, 
 University of Western Ontario 
 
 Les images suivantes ont ixi reproduites avec le 
 plus grand soin. compte tenu de la condition et 
 de la nertet* de l'exemplaire film*, et en 
 conformit* avec les conditions du contrat de 
 filmage. 
 
 Lea exemplaires originaux dont la couverture en 
 papier est imprimie sont fiimis er- commencant 
 par le premier plat et en termina- soit par la 
 derniAre page qui compone une empreinte 
 d'impression ou d'illustration, soit par le second 
 plat, selon le cas. Tous les autres exemplaires 
 originaux sont filmis en commencant par la 
 premiere page qui comporte une empreinte 
 d'impression ou d'illustration et en terminant par 
 la derniAre page qui comporte una teile 
 empreinte. 
 
 Un dee symboles suivants apparartra sur la 
 darniire image de cheque microfiche, selon le 
 cas: le symbole — ♦■ signifie "A SUIVRE ', le 
 symbole V signifie "FIN". 
 
 Maps, plates, charts, etc., may be filmed at 
 different reduction ratios. Those too largd to be 
 entirely included in one exposure are filmed 
 beginning in the upper left hand corner, left to 
 right and top to bottom, as many frames as 
 required. The following diagrams illustrate the 
 method: 
 
 Les cartes, planches, tableaux, etc.. peuvent etre 
 filmis A des taux de reduction diffirents. 
 Lorsque le document est trop grand pour etre 
 reproduit en un seul clich*. il est film6 d partir 
 de Tangle supirieur gauche, de gauche d droite, 
 et de haut en bas. en prenant le nombre 
 d'images nicessaire. Les diagrammes suivants 
 illustrent la m^thode. 
 
 1 
 
 2 
 
 3 
 
 1 
 
 2 
 
 3 
 
 4 
 
 5 
 
 6 
 
MICROCOPY RESOLUTION TEST CHART 
 
 ANSI and ISO TEST CHART No 2 
 
 1.0 ^^ 
 
 I.I 
 
 1 - 9 32 iiiji 2 2 
 2.0 
 1.8 
 
 1.25 
 
 1.4 i 1.6 
 
 js APPLIED IN/MGE Inc 
 
^ 
 
 ON THE GUANYLIC ACID OF THE SPEEEN 
 
 47996 
 
 W M.ll.K J0M;S as;, r., G. KOWNIKI !, 
 
 FR'IM 1H» I.\EOI!\r"RV -1 PllV^lnioolCM ClUMlvTBV INTHF [•HIS' H..PKI\« V' 
 
 !»..■ 
 
 I HI 
 
 \r.l. IV. Nc « 
 
 H'^ 'LOGIC \I. CIIKMISIRV 
 
• 
 
 ON THE GUANYLIC ACID OF THE SPLEEN. 
 
 Bv WALTER JOXES and L. G. ROWXTKKE. 
 
 (From the Laboratory oj Phviioloi^ical Chemistry in the Johns Hopkins 
 
 V ni: :rsity.) 
 
 (Receivcil for publication, February ni, n)o>.l 
 
 Ten years ago, Ivar Ban,<j'- siu^'ccdeil in isolatinL; frnin nx pan- 
 creas a nuelcic acid uiiich diflcrcil in a remarkable wax frmn all 
 kn'iwn substances nf this idass. According to Bang the coin- 
 pound is characterized by the following pro])ertics. 
 
 (i) It contains a pentose group. 
 
 (2) It coniaiiis a glycerine grnuj) and yields gh'cerine phos- 
 phoric acid. The nucleic acid thus stamling as a connecting link 
 between the pentosans and the lecithins estrdjlishcs a scries of 
 jihysiul'igical I'heniieal rclatiuns wlii*.!) can scarceh' \)v found 
 elsewhere in the science. 
 
 (3) It contains an aniount of nitrogen and iihosphorus rela- 
 ti\-ely gri'ater ihan is the ease with other nutleic acids. 
 
 (4) It _\iclds (111 hydrolysis an excessiveaniount of guanin but 
 no trace of cither adenin orthyiiiin. On account of this striking 
 difference from other nucleic acids, the substance has rieei'.'ed the 
 name lif "guanylic J' id." 
 
 This woj-k which if true \\'ould constitute an unmistakable 
 advance in the scieiice has unfortunately been the subject of 
 ad\'ersc critiiisin of a kind from which one would scarcelv e.\pcct 
 a rci o\-(.r_\'. Thus \\ Furih,^ who has gi\'cu the subject most 
 careful attention, ^\as able to find among the split products 
 neither glycerine iior a rediuing earboliyilrate; he claims that the 
 nucleic acid }'iclds adenin as well as guanin ami that there is no 
 reason for making any dist inci ion lietween guanvlic acid and 
 thymonucleic acid. In consideration of a number of objections 
 ail leading to the same coiulusion one might reasonabh- supjiose 
 
 ' liang: Zcitschr. /. physiol. Client., .\xvi, p. i^^; xx\i, p. jii. 
 ' V, Ftirtl; and Jerusalem; Hofmeister's Heitrui^e, \, ji. 174. 
 
 2.S() 
 
290 
 
 Guanvlic Acid of the Spleen 
 
 that V. Furth's w, .rk wt niM finally dispose of the matter of Ruany- 
 lic aci'l. Rut SteiKlcl' in a very recent communication, takes an 
 entirelv«litt'ercni view. He notes that themeihod .,f y>re]'arati"n 
 emplovc.1 1)V V. 1-urih (propose.l by Bang and Raaschou-J leads 
 to a miclcic'acid cf the ordinary type, but that by followmg the 
 original method n{ Bang it is ].MSsible fi <.l.'ain fnan thox pan- 
 creas a true guanylic aci.l, i. e., an organic ranjiound containing 
 phosphorus which yields guanin but not adenin. Unfortunately 
 Steudel agrees with v. Furth that guanylic acid contains no 
 glvceritic group. 
 
 ' It is the purpose of this eimimunication to show that not only 
 is a true guanylic acid obtainable from <.x pancreas but iliat sub- 
 stances (.f this class are conlined ncitherto a single animal species 
 nor t'.> a single gland. 
 
 Tin- prkp.'lR.xtion- of the xt'ci.HOPROTHiN- OF Pifi's splei;n-. 
 
 Fourteen kilos of carefully trimmed and hnely grcnind fresh 
 tissue were thoroughly mixed in portions with 21 liters of cdd 
 distilled water, heated quickly to boiling and the solution liltereil 
 off. It is immaterial whether the lluid be filtered hot or after 
 cooling; in either case a fairly clear filtrate is obtained which by 
 repeated filtration through the same hlter becomes almost as 
 clear as water but ])ossesscs a very pale reddish tinge. If the 
 residue be jiresscd through linen a milky fluid results which we 
 have not been able to render clearer, either by repeated tiltration 
 or bv long continued centrifugation; it is therefore advisable to 
 avoid disturbing the residue in any way and to use n(i greater 
 amount of water for the extraction than that stated. This part 
 of the process was exceedingly more satisfactory than was the 
 case with beef pancreas (see below), where we were never able 
 to obtain anything better than a highly opalescent filtrate, 
 although the solution obtained from this gland is described by 
 various writers as perfectly v lear. 
 
 The neutral fluid when cool was trcateil with acetic acid up 
 to 5 to 10 per mille and the prcciT.itated gelatinous nucleojircleid 
 was allowed to subside over niglit. Tins nu(le< .profcid is so 
 
 'StciK-k-l: /ritschr. j. physi,'t. Ch, it. liii. ]'■ 53'l. 
 
 : Han^' and Raastliou: llofiiinstrr's !<< ilr.n'r. iv. y. 17;. 
 
Walter Jones and L. G. Rowntree 
 
 291 
 
 sirikm-lv dilTcrcnt in ,)hysira! vrnpcrtics fn.m the M,rrfsi,. ^ivlm- 
 liravv'tliieeulcnt sul>^;anfe i>i <>x pannvas that wc can scarcely 
 belicvc the tv/o substaiue^ i.lenii.Ml. The ch.u.ly lluid \vrs partly 
 (lecantc.l ami the remainder shari.ly reiuovecl after (e:itnfut;a- 
 tu.n. The micle.ipn-tei.l wliich in tliis c.mi.a. i form resetiibles a 
 preparation of staivli paste, was freed fmrn soluble impurities by 
 alternate solution in a minimal amount of caustic so.la and pre- 
 cipitation with the requisite aiiKiUnt of acetic aci^l. Suspended 
 matter was removed as far as possible from each alkaline solution 
 by Ions continued cent rifugat ion, and in the same manner the 
 precipi'tate.l nucleopr<,teid v/as sharply separated fr^-m the super- 
 natant lluid. After this operation had been repeate<l several 
 thnes a pro.luct was obtained which dissolved in a trace of alkali 
 and on precipitati(.n from the alkaline solution by acetic acid 
 left a perfectly clear fluid. The exceedingly gelatinous nucleo- 
 protei<l thus puritic.l was d.ehydratcd with alcohol and ether, but 
 unless this is done with the greatest care one will tmally obtain 
 a bnAvn sticky mass which is unsuitable for the work that t.jUows. 
 It IS necessary to begin with dilute alcohol (50 per cent) and to 
 replace this gradually until absolute alcohol is linally readied. 
 Th.' latter slvuld be repeatedly used and the material allowed to 
 stan.l for several <lays in well cooked flasks with frequent and 
 violent agitation. We mention the difficulty of dehydrating 
 this nucleoproteid because we encountered no such ditficuliy m 
 dealing with the nucleoproteid of ox pancreas and we regard this 
 very sinking diflerence as sufficient ground for assuming that the 
 tw.; nucleoproteids r.re not identical. This is however entirely 
 aside from the question of the identity of the two nucleic acids. 
 From 14 kilos of moist tissue after the sacrifice of relatively 
 large quantities of material in the interest of a pure product, 
 we finally obtained 64 grams of a perfectly dry pale yellow 
 powder. 
 
 THE l'REPAR.\TION OF Gf-^NVLIC ACID FROM THE NUCLEO- 
 PROTEID Ol" PIO'S SPLEEN. 
 
 The nucleoproteid was treated in portions of 12 grams each 
 with 150 cc. of 2 per cent caustic potash and heated for half an 
 hour in a vessel submerged in boiling water. The red fluid was 
 neutralized with acetic acid end whale hot filtered from a small 
 
2q: 
 
 Guanvlir Acid ot tlit' Spleen 
 
 (luantitv -f i-erkTilv lihuk inatuKil; l.ni wen after stan.liriK' 12 
 lin.irs iheiv uas nn ,W\n,^hinu -f •^uin\yVu- acid alth<.u-b the 
 snlutinii was iiiurh in., re o .nrciitralcd tlian that wliidi Han;,' 
 pivparc.l fnim y.imvcufi luulc .vr-uM. i Wc used nnly i5o_y- 
 „f J iKT .cut caustic ]H,!ash v.-i-on- Han- used 400 ce.i The 
 perte^lh- dear vell.Av lluid was then made faintly aeid with 
 aectie aeid and allowed Im stand <.ver ni-ht '."it there uas still nn 
 deposition of Kuanylic aeid. Several days later when we had 
 c.mehidedthat thisnuelenpn.teid yiel Is n-. sul.stanee eorn-pc.nd- 
 ini,' to paiui-eas truanyhe arid and after v. e had praetieally alian- 
 d/med the suhjeet, a vt^ry small deposit was noticed in ilie thud 
 and on tlie ad'dution of a few drops of aeetie aeul there was an 
 iinmed.iate and eojnous preeipitati. .n of while lloeeiilenl material 
 wliile i.raetieidlv all the eol,,nn- mattiM' remained in solution. 
 Tlie preeipilate'was tillered oil, dissohv ' in hot water and tlie 
 solution til'ered from a small amount of ins.'luMe -ramilar maie- 
 
 ,.,;,p On lin-. the lole yellow ihiid prompt ly deposited , uuany- 
 
 li^. a,i,l l,ut ..nrei.eatm- the process the yield soon became notiec- 
 al.lv siiudier ;is llie acetic acid was removed with the mother 
 li,p'i.,i-s. Tite addition of acetic a> id to anv of these tiltrates 
 causes tm iiiimeditiie precipitation of -uanvlic acid. This dif- 
 ference m liehavior of spleen ...uaiiylic aci-l ffoiil ptmcreas siKiny- 
 lie acid, nii-ht he explained by d.ilierences in the solvent J.ower 
 of the impurities in the two cases hut si-leen -uanylic acid retains 
 this i.ropertv after purification lo >.ucli an extent that we are 
 in. line 1 to t'hc opinion that tlio 1 wo nucleic acid^ are not iden- 
 liiaP The ori-inal neutral solution .u'enenilly tillers Mowly hut 
 eontmuouslv and as a deposition of -uaiiyhc acid is not likely, 
 the slov.ncNS of the hltnition is with.out con<e,pience. Hut it 
 mav hapi^cn th.at the neutral fluid cannot he iiltere.l tit ;dl. In 
 the one such ease which we met the fluid was markedly aciditied 
 with .acetic acid, and co,,1ed in ice water when cletir and rapid 
 nitration could he made leavin- the -uaiiyli.- acid on the filter. 
 This dtirk l.rown residue was l.oiled with water and a product 
 obtained whi,'h wtis easilv tiltertible and which deposited K"anv- 
 lie acid on . oolin-. All specimens of what we considered the 
 best products were cllected and dissolved in hot water and the 
 guanvlic acid which was deposited .,n coojin- the fluid was dried 
 in tlie ordinarv wav, witli alcohol and eth.er. From 5J -rtiiiis of 
 
 \ 
 
 I 
 
Waller Jones and L. Ci. Rnwntree 
 
 293 
 
 \ 
 
 I 
 
 K 
 
 mu'lc'.prnteia after -cvere lussrs m the inuthcr l:(iuors f^r the 
 reason state.] ue imally ..btaiiie^ i.;S 1,'ram of pure jjuanylie 
 aeid. The suhstan> e e<msists of a perfeetly white dry j>ow.ler 
 soluble in hot water f..rming a transiKirent liquid whieh has an 
 arid reaction to litmus. It responds neither to the buiret nfir to 
 Millon's reaction bu' contains phosphorus and exhibi.s .ueneial 
 ].roperties and reactions '.vlii( h closely accord with those whim 
 Bang describes for the tjuanylic acid of the i)ancreas. 
 
 THE PfKIX liASKS PRODfC K I) HV HYDROLYSIS or Tlli-: GUAN'YLIC 
 ACIU Ol' I'IC.'s SPLKHN. 
 
 Owing to the misf.irtune of not knowin- in the earlier part of 
 our work that larf,'e (juantities of -uanylic acid may be recovered 
 from mother liquors by thcadditi^ai of acetic acid, the amount of 
 material at our disposal was insulficient for an exhaustive exam- 
 ination such as we ^vould otherwise have made and as we intend 
 to make in the immc^iiate future; so that we decided to devoie 
 all of our material to a tinal decision of the very important (|ues- 
 tion, whether or nc^t we are here dealing with a true guanylic 
 aciil. 
 
 A uram and a half ..f the material was heated for three hours 
 ^vitlil^s cc. of 5 per ( ent sulphuri..' acid in a vessel submerged in 
 boiling water. (Jn stan.ling over niglit the lluid deposited in 
 ])rofusion macroscopic needles of guanin sulphate. These were 
 dissolved by warming and the S(rlution was first neutralized and 
 then treated with such an excess of ammonia that the fluid con- 
 tained 2 per cent of the reagent. The jiroducl after digestion 
 in the warm for an hour was allowed to cool and the p..-ecipitated 
 guanin liltered off. After thoroughly washing in turn with i 
 I)er cent ammonia and water the base was dissolved in i per cent 
 caustic s(jda and again prcciidtated by the addition of acetic 
 acid. The precipitate was filtered off, washed, dried ami weighed. 
 For tlie separation (if guanin from small quantities of adenin 
 there are two properties of the liases whiJi can be used ami can 
 be thoroughly depended ujion. First, gu.anin is almost insoluble 
 in 2 per cent ammonia while adenin diss.ilves in this reagent 
 with comparative case. Second, both bases dissolve easily m 
 dilute caustic soda but while guanin is quantitatively precipitated 
 
Guanylir Acid of the Spleen 
 
 fn,m '^uJ^ a s,.lut,nn bv ac-cii-- a.ul, adcnin remain, mvhr th.^se 
 
 f these methois were ar:«Hcd u, n.rn to tl,e case wh.ch w.. a 
 descnbin,. The original amnv.n.aal f.Urate from .uan.n an. 
 the acetic aci.l tlui-l ubtame-l in its puriiicaii..n were urn: e.l an,l 
 treate<l with silver nitrate and ammcmia. The small silver pie- 
 cipitate was th..rou,l-.lv washed, suspended m boding watc^ and 
 Xosed with hvdrochlonc acid. Th.e a. id lUiid was .dtere, 
 from silver chloride, evaporated carefully just to dryness and 
 the last traces ..f hydrochloric acid driven ot. by moistenmg ^Mtl 
 ■ater and n,ain caa-fully evaporating. The insignihcant amount 
 of esidue w;s dissolved in water at 40° and treated withammon.a. 
 A v" stnall precipitate of guanin was forntcd which showed no 
 inclination to dissolve in ammonia even afuT the ,M...... • - 
 
 ,.reat excess of the reagent. The lluid was nltered off an. l-dcd 
 tint pcrtectlv neutral to litmus. I. will be observe.l that any 
 ad nirori.inallv present must now be foutid in this .lu,d ts 
 volume w;; <.nlv .0 cc. yet a T>ortion failed to give a distinct pie- 
 ciphate wi,h sih-er nitrate and ammonia whil.. in an^ 'her portion 
 pi,ric acid did not even produce even an opalescence. I lu 
 pu,v vUc nad of the sf^lccu gives no trace of ador.n. 
 
 The main vield <,f guanin together with the small amou, 
 obtained from the mother liqu-.rs weighed ;,„o ,m hgrams. 1 
 Zl issolved in hot 5 per cent hydrochloru- acid and decolorized 
 with a small an.ount of animal charcoal. The solution on cool ng 
 deposited the characteristic centimeter-long feathery nee. e 
 guanin hvdrochloratc. Thesal, w,.s all-.wcd .-, drv ,n the an and 
 analyzed' with the f..llowing results. 
 
 , o ■ - w Rrani lost ocSo gram at , 00^ and rcuirc.l 706 cc. of stand- 
 ard sulphuric acid >> cc. « 0.0077 gram ol ">\^"«^";. , ^^ ^,,^ 
 
 ,. o.'u.M pram lost o. 0,^0.. gram at loc and rciu.red ,.H,, cc. ot 
 same sulphuric acid. 
 
 ,■ II s ., ,. IICI. .'11.. o: I. 
 
 !, ir p; 11-- tiiT cent. 
 
 \'.. .:a.:n " • ' -' ' 
 
 It mav appear that our eonclusum. viz: that we are here deal- 
 ing uith a guanvlic acid, is base.l ..n an experiment with rath r 
 a ntall amount 'of material. In atiswer to such an objection .e 
 
Walter jcno and L. C Rcwntree 295 
 
 w,mM state that ^,c sh.,uM uso rm m,,re if wo wvr. tn n^eat the 
 ,vnrk wuh an unUnnted stn-ply "( '"^"^"='1 ^'1 V^"" '■.'^l'"';'; , ''^ 
 arc n,.v cn.auc.' in an inve^n.ati.n of nude.c acMs M .n- 
 volves a lar,e't.un.bcr of ju.t such an-Oyses as tha, ,losonbcd uvl 
 where the amount of material is of no consi.lcrai. on to us. Kx,;t- 
 ri en e in these cases has tau.ht us tha, the best quantuat.v 
 results can bo obtah^ed by us,n, no n.oro nuokno ao.l than w,ll 
 pnuluoe 300 to 400 milligrams of the base sou-ht. 
 
 ON THE msTRiiu-nox or (U-anvlic Ann in i.n: okoan.sm. 
 We arc now ocoupicl with the oxanunatiou of . nundK," ot 
 .lands for substances of this typo and hav. "";'"-;>,";;';; 
 Substances whose ,hys.cal propert,cs curres,K:.nd ^'l'- -; 
 those of KuanyUc acid. Thepig's pancreas yields ^ I-^'-"> ;^; 
 aqueous extract from which acetic acid prccpuates a tloccu ont 
 ,^ ,,U„,,,.,.,cid wlhch closely resembles the nucleoprotcd o ox 
 ancrcls and from which a ,uanvlic a, id can be prepared .huh 
 !,.„„„ be distinguished by anv apparent dhference from ox 
 ,,,„,,vas ^uanvhc a.id. Wuh ox pancreas we exponencod oi- 
 lid:rable dhhculty. The .lands used wore perfoctlv trosh and to 
 „,ethod ,nvcn bv Ban. cdosely foU-.-od bu, m spue o, ex c 
 eilort w;were unable to prepare any Mnn, approach.n, a d. u 
 aqueous extra,,. However, the cloudv lUud ,u>ves a nucleop,o- 
 tcid which in turn yu'lds a true Kuanvhc acid. 
 
 Our results show conclusively , ha, ,uanv.. ac,d f. ,r t ho ,>,.>- 
 H, ands') are considc,-ablv ,n.rc w.lolv d,s,nbu,cd , ,n a 
 
 f,.rmerlv supposed and lend m -Mva, n,oasu,-c ,0 'l:o bohot h.a 
 ,lu.se substances are conmu.n nuclear cons,„uen,s. As a . - - 
 ,,,,n< >,f orduKU-v nu, Ice aod ln,herto prepa,-cd have boon lound 
 ,„ ,,,,,1 „,,n,n as .oil as ,nan,n „ seems cor,a,n ,hat ,he .lands 
 winch veld ,uanyhca.>d nu>s, also con,a,n e.thcr adcn,hc 
 arid" ,',rnaclc.oa,,ds whuh pn.iuccbo,h bases. 
 
 S,ncewri,m. the above ar„.]o our a„cnt,.m has been called 
 t,, ,he work of Odcn.us^ who prepared .uanyhc a. .d Iron. ,he 
 ,ni> loopnitiid .if ,he mammary .land. 
 
 difrlnccs amen, the nucleic acids of this class, thus cstahhshu., a s.rus 
 
 of jjuanvlic acids. 
 
 'Sec Mulys Jahn-sbrrulU. sss:s. u)oo. 
 
niEK!" or WII.I.IAMK * W1I.«IN» (•.Mr»VT 
 BAI.TIMORR