key: cord-1053891-e1dl3qsr
authors: Wang, Leyi; Zhang, Yan; Byrum, Beverly
title: Complete Genome Sequence of Porcine Coronavirus HKU15 Strain IN2847 from the United States
date: 2014-04-17
journal: Genome Announc
DOI: 10.1128/genomea.00291-14
sha: 5e022a5ce7ca2c5463c52d20ed1a1e63259c17a7
doc_id: 1053891
cord_uid: e1dl3qsr

Porcine coronavirus HKU15 (PorCoV HKU15) was first detected in pigs with clinical diseases in February 2014 in the United States. Here, we report the complete genome sequence of Indiana strain IN2847, which might be useful for understanding the molecular profile of PorCoV HKU15.

the Deltacoronavirus genus of the Coronaviridae family, is an enveloped, positive-sense, single-strand RNA virus (1) . Previously, only one surveillance study reported detection of PorCoV HKU15 in pigs in Hong Kong (1) . It has never been associated with clinical diseases in pigs. On 7 February 2014, a deltacoronavirus (porcine coronavirus HKU15-OH1987) was detected in fecal and small intestine samples from a pig farm in Ohio (unpublished data). Subsequently, the whole genome of the coronavirus was sequenced and the virus was determined to be PorCoV HKU15 (L. Wang, B. Byrum, and Y. Zhang, submitted for publication). Here, we report the whole genome sequence of another PorCoV HKU15 strain, IN2847, which has been detected in Indiana.

Three fecal samples and an intestine sample from pigs experiencing an outbreak of a diarrheal disease at a farm located in Indiana were submitted to the Animal Disease Diagnostic Laboratory, Ohio Department of Agriculture. RNA samples were extracted and tested for porcine epidemic diarrhea virus (PEDV) and PorCoV HKU15. All samples were positive for PorCoV In conclusion, PorCoV HKU15 Indiana strain IN2847 is similar in genetic profile to another U.S. strain of PorCoV HKU15, OH1987, detected in Ohio. The sequence data suggest that both U.S. strains of PorCoV HKU15 were introduced into the United States by a common source. Further study of the virus from other geographic locations in the United States will facilitate our understanding of the genetic diversity and evolution of PorCoV HKU15 in the United States.

Nucleotide sequence accession number. The complete genome sequence of PorCoV HKU15 strain IN2847 was submitted to GenBank under the accession no. KJ569769.

Discovery of seven novel mammalian and avian coronaviruses in the genus Deltacoronavirus supports bat coronaviruses as the gene source of alphacoronavirus and betacoronavirus and avian coronaviruses as the gene source of Gammacoronavirus and Deltacoronavirus

Genome Announcements

We acknowledge and appreciate the excellent technical help provided by Jason Herr and Kerri Lawrence.This study was supported by the Ohio Department of Agriculture.