key: cord-1052531-9e8w1lbb authors: Garrido, Pedro; Cueto, Pitter; Rovira, Conxita; Garcia, Elisabeth; Parra, Ana; Enriquez, Raquel; Pinos, Armando; Sosa, Manuel; Hernández-Aguilera, Anna; Vallverdú, Immaculada title: Clinical value of procalcitonin in critically ill patients infected by SARS-CoV-2 date: 2020-11-08 journal: Am J Emerg Med DOI: 10.1016/j.ajem.2020.11.011 sha: 98cac827c8281d9a1a091d814309f0c94b821c7f doc_id: 1052531 cord_uid: 9e8w1lbb Background Blood procalcitonin (PCT) levels usually increase during infectious diseases and might be helpful to differentiate bacterial from non-bacterial origin. COVID-19 patients could present co-infections at initial presentation in the Emergency Department and nosocomial infections during stay in the ICU. However, the published literature has not established whether PCT changes could aid in the diagnosis of infectious complication during the COVID-19 pandemic. Methods Retrospective, single-center, cohort study, including COVID-19 patients admitted between March and May 2020. The data were prospectively collected for department purposes; laboratory results were collected automatically at admission and during the whole patient admission. Results 56 patients were analyzed (female 32%, male 68%), 35 were admitted to ICU, and 21 received general ward care. 21 ICU patients underwent mechanical ventilation (88%), and 9 died during admission (26%). Non-survivors had higher initial blood PCT levels than survivors at ICU admission (p. Patients admitted to Intensive Care Units (ICU) are at risk of nosocomial infections. Sepsis is a life-threatening organ dysfunction due to a dysregulated host response to infection (1) that requires early diagnosis, appropriate antibiotic treatment and other prompt measures such as intravenous fluids, vasopressor therapy and oxygen supply, as any delay may portend poor outcomes. It is established that unnecessary use of antibiotics increases antibiotic resistance, adverse effects and healthcare costs (2, 3) . Sometimes, sepsis appears with heterogeneous, or even markedly ambiguous manifestations, Procalcitonin (PCT) is a biomarker that has been proposed as a helpful tool in these situations (4) . PCT is produced by C thyroid cells with no hormonal activity, and by neuroendocrine pulmonary cells (5) in response to endotoxin bacterial stimuli and the release of interleukin-1 (IL-1) and tumor necrosis factor-alpha (TNF-α). A range of studies endorse the use of PCT in ICU patients as an effective diagnostic tool in sepsis and a prognosis marker (6, 7) , as plasma levels increase rapidly in 3 to 6 hours and reach their peak in 12-48 hours in response to bacterial infection (8, 9) . Viral infections usually result to low blood PCT level, due to inhibition of TNF-α production and increase of interferon-gamma (IFN-γ) that inhibits PCT synthesis (5, 8) . In addition, PCT has a low sensitivity for the diagnosis of bacterial infection in patients with renal failure (10) . dialysis (usually continuous renal replacement therapy) (12, 13) . At this stage, various studies have reported that PCT levels may have prognostic value (14) (15) (16) (17) but their value as a diagnostic marker of nosocomial bacterial infection in critically ill COVID-19 patients remains unknown. Our objective has been to investigate whether blood PCT level is a useful marker of bacterial coinfection at Emergency Department (ED) and nosocomial bacterial infection in ICU patients with pneumonia due to SARS-CoV2. We performed a retrospective, observational, cohort study in patients admitted to our hospital during March-May 2020 with COVID-19. All patients met the diagnosis criteria of pneumonia due to SARS-CoV2 defined as a positive result on real-time reverse transcription-polymerase chain reaction (RT-PCR) test of nasopharyngeal swab specimens and compatible positive chest imaging test (X-ray or computerized tomography). Patients under the age of 18, pregnant women, or with do-not-resuscitate (DNR) or do-notintubate (DNI) orders were excluded from this study. Demographic data and prognostic indices were obtained on admission to the hospital. These included the Simplified Acute Physiology Score (SAPS II) (18) and the Sequential Organ Failure Assessment scores (SOFA) (19) , both scores being calculated within the first 24 hours of admission. Blood PCT levels were measured at admission and then prospectively whether there was a suspicion of a bacterial infection. All the laboratory test results were collected automatically by our critical care information system (IntelliSpace Critical Care and Anesthesia, Philips, Germany) during the whole admission. The glomerular filtration rate (GFR) was calculated by the Modification of Diet in Renal Disease formula (MDRD-7) (21). We also collected data regarding the need for mechanical ventilation (invasive and noninvasive), renal replacement therapy (did you mean renal replacement therapy here or the actual technique = hemodialysis vs hemofiltration etc here?), and patient survival. Respiratory bacterial co-infection was defined as an infection diagnosed with one or more positive cultures obtained from blood, valid sputum, tracheal aspirate or pleural effusion at hospital admission of patients with COVID-19. Antigen test for detection of S. pneumoniae and L. pneumophila in urine and serum samples for serological tests for M. pneumoniae, C. pneumoniae and L. pneumoniae were also used. Microbiologically documented nosocomial infection was defined as an illness caused by organ invading pathogens with compatible clinical symptoms, imaging or laboratory findings and a positive culture, which justified the use of antibiotics. The study was approved by the Ethics Committee of the hospital (Institut d'Investigació Sanitària Pere Virgili Ref. CEIM: 168/2020). Patient's identification remained anonymous and informed consent was waived due to the retrospective nature of the study. Results are shown as means and medians with their respective standard deviations and interquartile ranges. The normal distribution of the variables was determined by the Shapiro-Wilk test. Comparisons between means were performed with the Student t-test or the Mann-Whitney test, when appropriate. Pearson or Spearman coefficients were used to evaluate the correlations between different variables. The interactions between variables were analyzed by multiple linear and binary logistic regression, incorporating confounding factors. Differences in p values less than 0.05 were considered statistically significant. The statistical analyses were performed with the SPSS 24.0 program (Chicago, USA). During the study period 56 patients with acute pneumonia due to SARS-CoV2 were included (67.9% males, 32.1% females), 35 required admission to ICU and 29 (82.9%) of them underwent invasive ventilation. The remaining 21 patients were treated in the general ward. Median (IQR 25-75) age was 68 years (60.0-73.8), median (IQR 25-75) blood PCT level was 0.22ng/ml (0.06-0.63) and mean (SD) estimated GFR was 69ml/min/1.73m2 (48-89). The general characteristics of the study population with and without ICU admission are shown in Table 1 . Patients admitted to ICU differed significantly blood PCT levels (0.30ng/ml vs 0.06ng/ml) ( Figure 1 ) and estimated GFR (88ml/min/1.73m2 vs 57ml/min/1.73m2) ( Figure 2 ) from those who entered general ward. We found an inverse correlation between blood PCT levels and estimated GFR at hospital admission (p <.05) ( Figure 3 ). The multiple linear regression analysis did not show any significant confounding factor in this linear association. Nine of the patients admitted to the ICU died during admission. Medians (IQR 25-75) for age, initial blood PCT level and estimated GFR were 66 years (59-71), 0.31 ng/ml (0.17-0.67), and 61.9 ml/min/1.73m2 (30-79) respectively. The characteristics of ICU patients at admission are shown in Table 2 . Non-survivors had a higher initial blood PCT level (median, IQR 25-75: 0.67ng/ml, 0.42-5.96) than survivors (median, IQR 25.75: 0.28 ng/ml, 0.09-0.64) with a significant positive nonparametric correlation (p<.006). 21 ICU patients (60%) received antibiotic therapy on suspicion of 31 bacterial nosocomial infections, some patients were suspected of more than one nosocomial infection, and in 15 (42.9%) at least one infection was microbiologically confirmed. 31 empirical antibiotic treatments were initiated because of suspected nosocomial infections and 25 of them were microbiologically confirmed. The empirical antibiotic therapy was adequate in 22 of the confirmed infections (88%). The blood PCT levels at the moment of diagnosis or blood PCT level variations from the day-before and the day-after the diagnosis did not significantly differ between those with confirmed infection and the non-confirmed cases, as shown in Table 3 , Figure 4 . We also did not find difference in the GFR between the two groups. The normal blood PCT level in a non-infected individual is 0.033ng/ml (±0.003ng/ml) (21) and is undetectable by the commonly used methods in clinical laboratories. Blood PCT levels below the detection limit of the assay offer excellent negative predictive value for bacterial community-acquired respiratory infection (22) and higher values are a useful guide to start and maintain antibiotic therapy in patients with acute respiratory failure (17) . In critically ill patients, PCT is recommended as a diagnostic biomarker of infection in febrile population and is associated with mortality (6). It has recently been used for early diagnosis of sepsis in these patients (25, 26) . In patients with ARDS, it has been proposed that blood PCT values above the usually accepted cut-off rule out a non-septic cause (27) . Hence, PCT emerges as a useful tool to establish antibiotic therapeutic algorithms (24) . As in our experience, the vast majority of studies show low initial blood PCT levels (16, 17, 27, 28) in non-severely ill COVID-19 patients admitted to hospital. Our ICU patients had higher blood PCT levels than those admitted to the general ward, which suggest the possibility of PCT being an early marker of disease severity or associated comorbidities. Furthermore, we observed higher blood PCT levels in those patients who died (0.67ng/ml) in concordance with other authors (15) (16) (17) 27, 29) and confirming the utility of initial blood PCT level as a marker of mortality. Low blood PCT levels are associated with a negative predictive value of 94% for bacterial infection in critically ill patients infected by Influenza A (30). Furthermore, a low incidence of co-infection has been documented in COVID-19 patients and low PCT levels (28) . Even though initial PCT high levels were found at ICU admission in comparison to non-ICU patients, we did not detect episodes of bacterial co-infection. Nevertheless, at ICU admission 91.4% of our patients were empirically treated with ceftriaxone plus azithromycin for 5 to 7 days due to the possibility of community-acquired bacterial pneumonia. After the first week of ICU admission, 60% (21/35) of patients received empirical antibiotic treatment (usually meropenem plus linezolid) on suspicion of nosocomial bacterial infection. We recorded 25 episodes of microbiologically-diagnosed nosocomial infections in 15 out of 35 patients. Comparisons of sequential results of PCT in patients with bacterial infection did not show a significant difference among the day-before, the day-after and the day of the nosocomial infection diagnosis. In a context of patients on mechanical ventilation due to severe hypoxemia, continuous fever, multiple inserted devices and diffuse pulmonary opacities in chest X-ray, PCT did not help to identify cases of nosocomial infection. SARS-CoV and SARS-CoV-2 invade human cells by using angiotensin-converting enzyme 2 (ACE2) as the entry receptor. ACE2 is amply found in the alveolar epithelium (31, 32) and the renal tubule. Detection of antibodies against nucleoproteins of virus (33) and anatomopathological kidney changes (34) demonstrate cell invasion in kidney structures. Hirsch et al (12) confirm that 36.6% of the hospitalized patients present some degree of renal failure, 31.1% of which are acute kidney injury (AKI) III and 14.3% need dialysis (96.8% of them require mechanical ventilation). Renal failure is associated with a high risk of mortality (35) and acts as a marker of worse prognosis (36). Our ICU patients had worse oxygenation (82% underwent mechanical ventilation during the first 24 hours of admission) and renal function in comparison to patients admitted at the general ward (57 vs 88 ml/min/1.73m 2 ) (Figure 2 ). In our series, 7 patients (20%) required dialysis. High blood PCT levels are described in patients undergoing chronic hemodialysis in relation to a chronic systemic inflammatory state (monocytes activation and subsequent inflammatory cytokines release) (37). We did not find any differences in renal function parameters between patients with and without nosocomial infections. However, we found a significant correlation between high blood PCT levels and worse renal function. This disclosure was independent from the presence of sepsis, invalidating it as a reliable marker in patients with renal failure when conventional cut-off concentrations are used (38) . A recent study reveals that blood PCT levels appeared to be disease severity-dependent in the COVID-19 pandemic. Notably, co-infection rates were only 20% and 50% in severe and critically ill patients, whereas high blood PCT levels' rates were 50% and 80% respectively (39). It seems contradictory that a viral infection may occur with high blood PCT level without concomitant bacterial infection, however severely ill COVID-19 patients are known to have high blood PCT levels (14) (15) (16) 24, 29, 39) . In this scenario, they frequently suffer organ failures, e.g. kidney failure, which is another target of this infection (11, 35, 36) . PCT has a low sensitivity for the diagnosis of bacterial infection in patients with renal failure (10), hence we believe that there may be a link between the kidney failure and high blood PCT levels found in these patients, that hinders the identification of a possible bacterial infection. Our study has some limitations. First, it is a single-center retrospective study limited by the sample size. Second, we do not have a procalcitonin-guided antibiotic stewardship protocol, therefore, PCT assays are not routinely used and were measured at the clinician's discretion. Third, we do not know whether blood PCT levels were influenced by the massive use of empirical antibiotic therapy. Our study provide evidence that initial blood PCT level at Emergency Department in COVID-19 patients is a useful marker of severity and poor prognosis. We also found that higher blood PCT levels at ICU admission of these patients were associate with higher mortality. These findings suggest that blood PCT levels could be used (in addition to other measures) for triaging critically ill patients in the ED. During stay in the ICU, blood PCT levels were high in COVID-19 patients and variations in serial blood PCT levels did not help to detect bacterial nosocomial infections. We found an inverse correlation between PCT concentrations and GFR estimation. The impaired renal function may have played a role in the high serum PCT concentrations detected. Further studies are needed to establish the impact of these findings. All the laboratory test results were collected automatically by our critical care information system (IntelliSpace Critical Care and Anesthesia, Philips, Germany) during the whole admission. Laboratory tests were analyzed by standard test in Roche/Hitachi Modular Analytics P800 System (Roche Diagnostics, Basel, Switzerland). For the real-time RT-PCR RNA was extracted from clinical samples (140µL) on a Qiacube using the Rneasy Mini kit (Qiagen)) following a viral inactivation step using Lysis Buffer according to the manufacturer's instructions. RNA elution occurred in 50µL RNAse free water and 5 µL were used for the RT-PCR. Reverse transcription and RT-PCR were performed on an LC480 thermocycler (Roche) based on Corman et al. (40) protocol for the detection of RdRP and E genes using the Taqman Fast Virus 1-StepMaster Mix (Thermo Fisher). Primers and probes (Eurogentec, Belgium) were used as described by the authors (40). The glomerular filtration rate (GFR) was calculated by the Modification of Diet in Renal Disease formula (MDRD) (20) . 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Open Forum Infect Dis We acknowledge all health-care workers involved in the treatment of our patients, and we thank Jordi Camps (Institut d'Investigació Sanitària Pere Virgili, Universitat Rovira i Virgili, Reus, Spain) and Gabor Zilahi (St George's Hospital, London, UK) for review of the manuscript. The authors received no specific funding for this work. The authors have no conflicts of interest to declare.