key: cord-1052050-ljf0p67k
authors: Ribeiro, Deidiane Elisa; Oliveira-Giacomelli, Ágatha; Glaser, Talita; Arnaud-Sampaio, Vanessa F.; Andrejew, Roberta; Dieckmann, Luiz; Baranova, Juliana; Lameu, Claudiana; Ratajczak, Mariusz Z.; Ulrich, Henning
title: Hyperactivation of P2X7 receptors as a culprit of COVID-19 neuropathology
date: 2020-12-16
journal: Mol Psychiatry
DOI: 10.1038/s41380-020-00965-3
sha: 242c47b645b8c05ed3daba3aac1ae81891238439
doc_id: 1052050
cord_uid: ljf0p67k

Scientists and health professionals are exhaustively trying to contain the coronavirus disease 2019 (COVID-19) pandemic by elucidating viral invasion mechanisms, possible drugs to prevent viral infection/replication, and health cares to minimize individual exposure. Although neurological symptoms are being reported worldwide, neural acute and long-term consequences of SARS-CoV-2 are still unknown. COVID-19 complications are associated with exacerbated immunoinflammatory responses to SARS-CoV-2 invasion. In this scenario, pro-inflammatory factors are intensely released into the bloodstream, causing the so-called “cytokine storm”. Both pro-inflammatory factors and viruses may cross the blood–brain barrier and enter the central nervous system, activating neuroinflammatory responses accompanied by hemorrhagic lesions and neuronal impairment, which are largely described processes in psychiatric disorders and neurodegenerative diseases. Therefore, SARS-CoV-2 infection could trigger and/or worse brain diseases. Moreover, patients with central nervous system disorders associated to neuroimmune activation (e.g. depression, Parkinson’s and Alzheimer’s disease) may present increased susceptibility to SARS-CoV-2 infection and/or achieve severe conditions. Elevated levels of extracellular ATP induced by SARS-CoV-2 infection may trigger hyperactivation of P2X7 receptors leading to NLRP3 inflammasome stimulation as a key mediator of neuroinvasion and consequent neuroinflammatory processes, as observed in psychiatric disorders and neurodegenerative diseases. In this context, P2X7 receptor antagonism could be a promising strategy to prevent or treat neurological complications in COVID-19 patients.

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in December 2019 as the infectious agent of coronavirus disease 2019 (COVID-19).

SARS-CoV-2 invades cells using prominent spike protein, which binds to cellular membrane receptors. Host cell receptors recognized by SARS-CoV-2 spike proteins include angiotensin-converting enzyme 2 (ACE2) [1, 2] and CD147 (basigin) [3, 4] besides involving virus spike protein priming/processing by transmembrane serine protease 2 (TMPRSS2) [5] . SARS-CoV-2 enters the cell through receptor-mediated endocytosis or receptorindependent entry, as shown for HEK293/hACE2 cells [6] . The challenging question is, whether the infection can occur through extracellular microvesicles shed from infected cells. Such mechanism is often called "trojan horse" and has been proposed for human immunodeficiency virus (HIV) [7, 8] . Although neural acute and longterm consequences of SARS-CoV-2 infection are still unknown, neurological symptoms are being reported worldwide. Therefore, urgent challenges are to identify, ameliorate, or even eliminate these effects. COVID-19 complications are widely associated with exacerbated immunoinflammatory responses upon SARS-CoV-2 lung invasion. In this scenario, pro-inflammatory factors are intensely released, the so-called "cytokine storm", which enter the bloodstream, and reach other organs (Fig. 1A ) [9] . In this sense, both pro-inflammatory factors and viruses can cross the blood-brain barrier (BBB) and enter the central nervous system (CNS), initiating a neuroinflammatory process [10] . BBB permeability is increased in patients with neurodegenerative diseases, which could facilitate SARS-CoV-2 neuroinvasion. In addition, the neuroinflammatory insult could increase susceptibility to neurodegeneration in patients who are not yet suffering from these diseases. While confirmation of this hypothesis may take years, possible long-term consequences of COVID-19 need to be highlighted.

Neurotropism of human coronaviruses has already been demonstrated in small animals and in autoptic studies of brains infected with the severe acute respiratory syndrome coronavirus (SARS-CoV) [11] , which was responsible for the severe acute respiratory syndrome (SARS) outbreak during [2002] [2003] . Image studies [12, 13] and postmortem examination [14] of the brain of COVID-19 patients also revealed hemorrhagic lesions accompanied by neuronal injury and neuroinflammation signals. These brain pathologies are described as both etiological factors and consequences of psychiatric disorders and neurodegenerative diseases [15] . Therefore, it is plausible to suggest that patients with COVID-19 are prone to develop neurological disorders, as well as patients suffering from these conditions are more susceptible to SARS-CoV-2 infection. In this context, understanding the mechanisms underlying Fig. 1 Possible routes of SARS-CoV-2 infection of the central nervous system and cytokine storm involvement. A In the lung, SARS-CoV-2 may infect type I and II pneumocytes (dark and light blue, respectively) and proliferate in these cells. SARS-CoV-2 invades type I pneumocytes following binding to TMPRSS2 and possibly CD147 receptors, whereas invasion of type II pneumocytes additionally involves binding to ACE2 [171, 172] . The distressed cells release pro-inflammatory factors and DAMPs, such as ATP, which recruit macrophages (green) by activating P2X7 receptors. These macrophages increase the release of cytokines, chemokines, and ATP, inducing the cytokine storm. ATP could also activate P2X7 receptors in type I pneumocytes. These pro-inflammatory factors, as well as the virus, reach the circulatory system and can induce inflammatory responses in other tissues, in part through the activation of P2X7 receptors by released ATP. B In the neuronal retrograde pathway, the virus infects peripheral neurons and uses synaptic connections to reach the CNS. In the transcribrial pathway, SARS-CoV-2 can multiply in sustentacular cells (pink) of the nasal cavity, using ACE2 and TMPRSS2 receptors for infection or in olfactory sensory neurons (purple) following binding to CD147 receptors. Thus, the virus uses these neurons to reach the CNS olfactory bulb (yellow) and infect cells using ACE2 receptors [173] . Sustentacular cells express P2X7 receptors [174] . C Once in the bloodstream, the virus can reach and infect blood-brain barrier (BBB) endothelial cells (pink), through binding to ACE2 and CD147 [175] , and perivascular astrocytes (green), through binding to ACE2 [172] . In COVID-19, the BBB shows increased permeability due to the inflammatory process and death of endothelial cells and astrocytes, possibly resulting from P2X7 receptor activation.

Although not yet confirmed, the virus could infect monocytes (purple) using CD147 receptors [176] , which in turn can pass the BBB. Inside the CNS, the virus can infect neurons (blue) and other neural cell, by binding to ACE2 [173] and possibly to CD147 [177] . Moreover, cytokines present in circulating blood can also reach the CNS through BBB breaches and induce neuroinflammation, sensitizing the brain. Created with BioRender.com. COVID-19 neuropathology is essential for the development of therapeutic strategies.

Hyperactivation of P2X7 receptors is closely related to inflammatory processes since they are stimulated by ATP released from distressed cells and induce inflammasome activation [16] [17] [18] . P2X7 receptors are ATP-gated ion channels widely expressed in the CNS [19] , and their activation induced by viral infection leads to molecular (mainly neuroimmune response activation, reactive oxygen species (ROS) formation, and glutamate release) and behavioral alterations [17, 20] as well as to mental disorders [21] .

Based on this, we postulate that both, neuroinvasion through the BBB and hyperstimulation of neuroimmune responses observed during COVID-19 infection, are mediated by hyperactivation of the P2X7 receptor, possibly through NLRP3 inflammasome stimulation. This cascade could lead to COVID-19-associated psychiatric disorders and neurodegenerative diseases. Thus, P2X7 receptor antagonism would be a promising strategy to prevent or treat neurological complications in infected patients. This concept will be discussed in detail in our review.

An increasing number of studies reports the manifestation of neurological symptoms in patients with COVID-19. As reviewed by Pezzini and Padovani, neurological manifestations affect between 4.2 and 100% of studied patients: dizziness (prevalent in 7.6-46.1%), headache (5.1-77.1%), impaired consciousness (9.5-64.1%), and seizures (1.2-26%) [22] . Noteworthy, in COVID-19 patients presenting at least one neurological symptom, the prevalence of acute stroke (2.9-76.8%), confusion (14.2-65%), and encephalitis (0-27.9%) stands out. COVID-19 patients also present other nervous system symptoms, such as impaired taste (10-19.2%) and smell (6-21.7%), dysautonomia (4.3-12%), and acute inflammatory demyelinating polyneuropathy (0-16,2%) [22] .

Together with an acute cerebrovascular disorder, all symptoms are correlated with the consequences of strokes or micro-strokes [23] . Moreover, reported impaired olfaction could be due to CNS invasion or direct damage of olfactory sensory neurons in the nasal cavity [24] .

Psychiatric symptoms are being reported in COVID-19 patients, healthcare workers and in the general population. Table 1 summarizes meta-analysis and systematic reviews on these topics. The studies were selected after searching the PubMed data base on September 20, 2020 for the terms: "(COVID-19 or SARS-CoV-2 or severe acute respiratory syndrome coronavirus 2) and (mental health or psychological health or depression or anxiety or PTSD or PTSS or post-traumatic stress disorder or post-traumatic stress symptoms)".

Although studies on psychiatric symptoms accompanying SARS-CoV-2 illness need further investigation, the available data indicate that COVID-19 patients mainly present depressive symptoms (42-65%), anxiety (37-47%), and PTSS (93-96%). Noteworthy, stress caused by the pandemic situation (e.g., social isolation, fear of infection, and financial instability) may also induce depressive symptoms (14.6-48.3%), anxiety (6.33-50.9%), and PTSS (7-53.8%) in the general population. The stressful situation of healthcare workers, specially the fear to infect relatives, may lead to depressive symptoms (12.2-26.3%), anxiety (13-29%), and PTSS (3-20.7%) as well.

In this scenario, the following proposals are raised and require additional studies: (1) alterations induced by stress exposure (e.g., immune hyperactivation) could both facilitate SARS-CoV-2 infection as well as aggravate COVID-19 symptoms; (2) neurological symptoms are probably not specifically related to SARS-CoV-2 invasion, but they are a general consequence of infectious disorders (which include sickness behavior), stressful events (pandemic situation), and/or immune response hyperactivation ("cytokine storm"); (3) genetic and/or environmental factors affect the development of neurological symptoms, as these responses highly vary across the population. Clarifying these issues would improve the understanding on SARS-CoV-2 infection and direct the search for treatments.

COVID-19 patients are presenting neurological symptoms worldwide. Although brain analysis of these patients is not being widely performed, a study detected SARS-CoV-2 in 8 of 21 postmortem brain tissues, based on reverse transcriptase polymerase chain reaction [25] . Moreover, neuroinvasion capability of SARS-CoV-2 was observed in human brain organoids, especially in neuronal cells, such as neural progenitor and radial glial cells, accompanied by increased cell death [26] . The same study found positive staining for SARS-CoV-2 spike protein in the brain of three COVID-19 patients, with different expression patterns and staining intensities [26] . Neuroinvasion potential of SARS-CoV-2 was demonstrated in mice expressing human ACE2 and correlated with increased mortality independent from respiratory infection [26] . SARS-CoV-2 also seems to invade infants' brains, since immunohistochemical analysis of the postmortem brain of a 1-year-old infant showed positive staining in the choroid plexus, ventricles and cerebral cortex [27] . Remarkably, a case report demonstrated the presence of SARS-CoV-2 viral particles in neural tissue and brain capillary endothelium of a Parkinson's disease patient, which was associated with the worsening of neurological symptoms [28] .

However, the mechanism of SARS-CoV-2 infection of the brain is still unknown. Invasion routes of the CNS by other viruses include: (a) the hematogenous route, in which CI Confidence interval, OR Odds ratio, N.R. Not reported viruses use the bloodstream to reach and invade epithelial cells from the BBB or the blood-cerebrospinal fluid barrier, or use leukocytes as a vector to enter the CNS; (b) the neuronal retrograde route, in which viruses invade peripheral neurons and reach CNS, including the transcribrial route, using olfactory sensory neurons in the nasal cavity ( Fig. 1B ) [10] .

In the hematogenous route, the virus must be capable of crossing the BBB (Fig. 1C ). This barrier is composed of endothelial cells, pericytes, and astrocytes. The restricted permeability of the BBB is a reflex of the connection between brain microvascular endothelial cells and tight cell-cell junctions. The BBB is disrupted under inflammatory conditions [29, 30] . In the "Trojan horse" mechanism of CNS invasion, infected leukocytes pass the BBB. This mechanism is observed for HIV, and since SARS-CoV can infect immune cells, it is likely that SARS-CoV-2 also uses this route toward the CNS [10] . Viral infection also affects BBB integrity by different mechanisms, including phosphorylation of tight junction proteins, disruption of the basal lamina or of the actin cytoskeleton, or by invading BBBepithelial cells and furthermore astrocytes [10, 31] .

Evidence of CNS invasion through neuronal retrograde routes was reported for coronaviruses, such as HCoV-OC43, HEV67 and avian bronchitis virus [32] . Once respiratory and digestive tracts of animals were infected, coronaviruses invaded peripheral neurons and passed through synaptic connections until they reached medullary neurons and subsequently other neurons and glial cells of the CNS [32] . As the main entry route of SARS-CoV-2 in humans, cells from the nasal cavity could be susceptible to viral infection and replication. Studies reported that human olfactory sustentacular cells express both ACE2 and TMPRSS2 virus receptors [33] . Although current literature data reports that human olfactory sensory neurons do not express these proteins, they express CD147 that could allow SARS-CoV-2 neuroinvasion (Fig. 1) [33, 34] . In fact, SARS-CoV, MERS-CoV, and HCoV-OCR43 were able to invade the murine CNS using the transcribrial route, infecting olfactory sensory neurons of nasal cavity, and passing to other neural cells, indicating that SARS-CoV-2 could use the same mechanism [24, [35] [36] [37] .

Severe COVID-19 patients commonly develop the Acute Respiratory Distress Syndrome [38] , which is characterized by inflammatory injury to the alveoli-capillary membrane, leading to lung over-permeability and increased pulmonary edema fluid into the airspaces, resulting in the lack of respiratory capacity [39] . This overreaction of the innate immune system against viral infection induces the so-called "cytokine storm", comprising of: (1) the release of large amounts of several pro-inflammatory cytokines (interferons  IFNα and IFNγ, interleukins [IL-1β, IL-6, IL-12, IL-18 and  IL-33] , tumor necrosis factor [TNF]-α, and transforming growth factor [TGF]-β) and chemokines (CXCL10, CXCL8, CXCL9, CCL2, CCL3, CCL5); (2) release of renin-angiotensin aldosterone system (RAAS) mediators and increasing blood levels of angiotensin II (Ang II); and lately (3) amplification of the innate immune system response and activation of its major humoral arm, the complement cascade (ComC) [40] .

Novel evidence indicates that COVID-19-released mediators merge on a common pathway, upregulating cytosolic danger sensing pattern recognition receptor, which is part of a multiprotein complex of the innate immune system that is called inflammasome, and recognizes both pathogenassociated molecular patterns and self-derived dangerassociated molecular patterns (DAMPs) or alarmines [41] .

Importantly, upon inflammasome protein assembly and activation, pro-caspase 1 protein is cleaved to functional caspase 1, whose main function is the conversion of the inactive and intracellularly stored pro-inflammatory cytokines, pro-IL-1β and pro-IL-18, into their active forms that are released from cells. This release is facilitated by creating gasdermin D (GSDMD) pore channels in cell membranes. In addition to these two cytokines, gasdermin D channels also mediate the release of several biologically active DAMPs or alarmines, including extracellular ATP, high mobility group protein B1, and S100 calcium-binding proteins A8 and A9 (S1008/9a) [42] .

There are various inflammasome subtypes. The NLRP3 inflammasome protein complex is usually involved in virus infections and consists of NLRP3 protein, apoptosisassociated speck-like protein containing a CARD (ASC) and pro-caspase-1, and remains in the cytosol in steadystate conditions in an inactive form. Upon activation, it becomes a multiprotein aggregate composed of several NLRP3 molecules (speck complexes), each containing NLRP3 protein, ASC, and pro-caspase 1 [41] .

We consider the NLRP3 inflammasome as a trigger of the cytokine storm, as seen in COVID-19 patients, that may be induced by P2X7 receptor activation, including in the brain (Fig. 2) . Once SARS-CoV-2 spike protein interacts with ACE2, macrophages/microglia cells can potentiate the immune response through the cleavage of fragments complement component 3a and 5a (C3a and C5a, respectively) and non-lytic C5b-C9 membrane attack complex by ComC, thus activating the NLRP3 inflammasome [41] . Moreover, NLRP3 inflammasome activation during COVID-19 infection is usually triggered by Ang II, which binds to the angiotensin type 1 receptor (AT 1 R), leading to vessel contraction and increasing blood pressure. In normal conditions, Ang II is converted by the ACE2 receptor into angiotensin 1-7 (Ang 1-7) [43] . Ang II activates the AT 1 R, inducing fibrosis, increased ROS release, vasoconstriction, and gut dysbiosis. In contrast, Ang 1-7 binds to Mas receptors (MasR), thus protecting against fibrotic formation, presenting antioxidant and vasodilatory effects. The problem with the conversion of Ang II into Ang 1-7 is that the ACE2 enzyme expressed on surface of cells is a COVID-19 receptor and is blocked or downregulated after binding of virus or even shed from the cell surface [43] . Extracellular ATP can also robustly trigger activation of the NLRP3 inflammasome through P2X7 receptor activation. In addition, ATP is also released into the extracellular milieu upon NLRP3 inflammasome activation, promoting a strong positive feedback loop [44] .

SARS-CoV-2-induced systemic inflammatory responses may also result in endothelial damage and consequently increased production of thrombin by inflammatory cytokines during sepsis in a bidirectional way, because inflammation activates coagulation, and coagulation augments inflammatory activity [45] . In this scenario, active inflammatory cells expressing specific protease activated receptors A SARS-CoV-2 may alter brain function by reaching the central nervous system (described in Fig. 1 ) and/or through the cytokine storm-mediated effects. The result is a neuroinflammatory process characterized by microglia (pink) hyperactivation, astrocyte (green) stimulation, and demyelination (yellow caps) of neurons (blue). In addition, the cytokine storm induces bloodclot formation and increased capillary (red) permeability resulting in embolic and hemorrhagic strokes, respectively. B In a molecular view, the distressed cells release pro-inflammatory cytokines (yellow circles) and ATP (red circles). ATP activates P2X7 receptors (red) expressed mainly in microglia (pink) and astrocytes (green) resulting in increased Ca 2+ influx and glutamate (purple circles) release. Glutamate activates NMDA receptors expressed in nerve terminals (blue), which enable Ca 2+ -dependent exocytosis of ATP and more glutamate release. In this way, an auto regenerative loop is formed causing a massive release of these neurotransmitters augmenting excitotoxicity and cell death. In the postsynaptic neuron (blue), increased [Ca 2+ ] i leads to Ca 2+ -calmodulin (CaM) complex formation and consequent nNOS activation. NO production mediates neurotoxicity via several mechanisms. NO interacts with the iron-sulfur centers in the mitochondrial electron transport chain impairing cellular energy production. NO also produces reactive nitrogen species and reactive oxygen species (ROS). Reaction of NO and superoxide ion (O 2 − , formed by nNOS under low arginine concentrations) generates peroxynitrite (ONOO -) and peroxynitrous acid (ONOOH). These free radicals can also decompose into other reactive species, such as hydroxyl radical and peroxides.

Oxidative stress from free radicals includes DNA damage, lipid peroxidation, tyrosine nitration, and excess S-nitrosylation. These structural changes can lead to protein misfolding and aggregation causing neuronal impairment and/or death. In microglia, K + efflux mediated by P2X7 receptor activation may trigger NLRP3 inflammasome assembly and activation through NIMA-related serine/threonine kinase 7 (Nek7) binding. NLRP3 inflammasome mediates the activation of caspase-1, which induces the maturation of interleukins (IL) by cleaving pro-IL-1β and pro-IL-18 in IL-1β and IL-18, respectively. The mature forms of cytokines are secreted worsening the neuroinflammatory process established. C The hyperactivation of NPLR3 inflammasome and consequent release of cytokines and ATP can occur by different routes. (I) Activation of the renin-angiotensin system (RAS) leads to elevated levels of angiotensin II (Ang II) that binds to the AT 1 R receptor. (II) The N proteins of the SARS-CoV-2 virus activate ComC in a mannan binding lectin (MBL)-dependent manner, producing C3a and C5a anaphylatoxins and forming the non-lytic C5b/C9 membrane attack complex (MAC). ATP can also activate MBL and induce this response. (III) P2X7 receptor activation by ATP induces K + influx and inflammasome activation. (IV) SARS-CoV-2 invasion through ACE2, TMPRSS2 or CD147 activates the inflammasome in target cells. Hyperactivation of these pathways leads to activation of caspase 1, release of mature IL-1β and IL-18, the insertion of gasdermin D channels in the cell membrane and the release of danger-associated molecular pattern molecules (DAMPs), which amplify the innate immune response and may lead to cell death by pyroptosis. Created with BioRender.com.

(PAR1-4) bind to thrombin, while the Toll-like receptor 4 interacts with fibrin. Supporting these events, patients in particular at younger age are often diagnosed with stroke and cardiovascular complications.

Recent evidence indicates that the occurrence of psychiatric disorders in patients is linked to "sterile" inflammation of the brain that may be initiated locally by some stressors affecting nervous tissue or occurs due to a systemic inflammation process [17, 46] . This is supported by the observation that several inflammatory mediators and markers are detected in the peripheral blood of patients with psychiatric and neurodegenerative disorders [17, 46] , which could worsen prognoses for COVID-19 outcome. Moreover, clinical data describe correlations between systemic chronic inflammatory processes and psychiatric disorders. This may also explain, why some reported antiinflammatory treatment strategies ameliorate neurodegeneration [17, 46] . In agreement, pathological increase of AngII-AT 1 R-mediated activation of NLRP3 inflammasome may initiate psychosis. We believe that bioactive inflammatory mediators released during the cytokine storm, such as extracellular ATP, affect the CNS and may lead to its impairment, mainly through P2X7 receptor activation, as we discuss in the following.

The P2X7 receptor is widely expressed through the body [47] , including in immune [48] , lung [49] , and CNS cells, mainly microglia and oligodendrocytes [50] [51] [52] , whereas its expression in neurons and astrocytes is still under discussion [50, 51, [53] [54] [55] [56] [57] [58] [59] [60] . During the progress of infection, ATP release may result from the NLRP3 inflammasome response to COVID-19 infection (i) after virus spike protein interaction with the virus entry receptor ACE2, (ii) due to elevated level of Ang II observed in infected patients, or (iii) in response to activated ComC mediators [61] . Consequently, P2X7 receptors are activated, increasing inflammatory responses as well as modulating RAAS-related pathways and BBB permeability, as we discuss in the following.

The P2X7 receptor is a trimeric ionotropic receptor that belongs to the P2X family of purinergic receptors, presenting low affinity for ATP (EC 50 around 0.3-1.8 mM) and, under prolonged stimulation, can form pores allowing the passage of large hydrophilic molecules [62] [63] [64] . Activation of P2X7 receptor elicits rapid K + efflux as well as Ca 2+ and Na + influx, resulting in the stimulation of several intracellular intermediators, as PLC, PLA2, PKC, MAPK, PI3K, ERK1/2 and p38, among others [65] . Consequently, P2X7 receptor activation is associated with numerous cellular functions, as plasma membrane blebbing, phosphatidylserine exposure in the membrane, formation of ROS, interleukin secretion, cell death and proliferation [65, 66] .

ATP concentration is present in the nanomolar range in extracellular space of healthy tissues [67] . Conversely, in a disease state as infection or brain disease, extracellular ATP levels largely increase and activate P2X7 receptors [68] [69] [70] . Consequently, pore formation results in enhanced release of ATP and establishes a positive feedback in pathological conditions. In these circumstances, ATP may act as DAMP, which activates the nuclear transcription factor NF-κB resulting in expression upregulation of pro-IL-1β and pro-IL-18 and the NLRP3 protein [16, 71] . In addition, P2X7 receptor activation triggers K + efflux, a signal required for efficient NLRP3 inflammasome stimulation [72] . Consequently, there is an activation of caspase-1 and maturation of IL-1β and IL-18, resulting in pro-inflammatory cytokine release [16, 20, 52, 71, 73] . The P2X7 receptor is a major activator of the NLRP3 inflammasome in several cell types [16] , including macrophages and microglial cells [72, [74] [75] [76] . Moreover, P2X7 receptor activation also promotes the release of other inflammatory mediators such as IL-6, TNFα, CCL2, CCL3, and CXCL2 [77] [78] [79] [80] .

In fact, the role of P2X7 receptor as a modulator of inflammatory response is well-established [16, 70] . It was already reported its capacity to modulate acute and chronic infection [81, 82] , inflammatory diseases [82] , sepsis [83, 84] , neuropathic pain [85] , and T-cell activation [86] . In addition, P2X7 receptor expression deletion appears to be beneficial in case of acute lung injury, asthma, lung inflammation, and fibrosis [87] [88] [89] [90] [91] . Remarkably, P2X7 receptor activation increases neuroinflammatory responses, which have been associated with neurodegenerative diseases, psychiatric disorders and stroke [21, [92] [93] [94] [95] , as further discussed.

Cytokine storms have been reported in several viral infections [96] , including SARS-CoV-2 [9, 97] . Patients infected with SARS-CoV-2 present elevated levels of IL-6, IL-10, IL-1β, INF-γ, CCL2, TNF-α, CXCL10, CCL7, IL-1 receptor antagonist and IL-2 receptor, supposedly associated with disease severity [9, [98] [99] [100] [101] [102] [103] [104] [105] . Considering the P2X7 receptor role in inflammation, its activation may be involved in the cytokine storm observed in COVID-19 by stimulating NLRP3 inflammasome, overshooting inflammation with extensive cytokine release, affecting coagulation and leading to diffuse lung edema and infiltration by immune cells and inflammatory cytokines [106] . As discussed by Di Virgilio et al. [106] , many processes associated with lung impairment, as seen in COVID-19 patients, are mediated by P2X7 receptors [16] . The massive ATP release following lung mononuclear phagocytes invasion by SARS-CoV-2 can activate P2X7 receptors of antigen-presenting cells and macrophages, increasing cytokine, chemokine and ATP secretion [16] . In addition, P2X7 receptor knockout mice submitted to a model of lung fibrosis revealed reduced infiltration of inflammatory cells, cytokine release, apoptosis, and fibrosis, while P2X7 receptor agonists increased these deleterious processes [88, 107] .

Therefore, we hypothesize that the P2X7 receptor activation is involved in the cytokine storm and associated lung and brain inflammation caused by SARS-CoV-2 infection.

The P2X7 receptor, as a central player in inflammation, participates in viral infections. Exerting mostly proinflammatory roles [20] , the P2X7 receptor is important for pathogen elimination [108] , but on the other hand, its activation may be detrimental to the host due to the induction of exacerbated inflammatory responses. Protective roles of P2X7 receptor activation have been described, for instance, against vesicular stomatitis virus, Newcastle disease virus, murine leukemia virus and HSV virus infections [108] . Controversially to the above-cited beneficial P2X7 receptor-mediated viral elimination, this receptor was described to even facilitate virion release from HIV-infected macrophages in an exocytosis-dependent way [109] . Moreover, P2X7 receptor activation exacerbated inflammation, enhanced tissue damage, increased mortality, and worsened lung pathology involving P2X7 receptor expression or activation, such as observed in several other studies with e.g., influenza A virus [110] and adenovirus [111] .

Importantly, the P2X7 receptor triggers pathways related to the functioning of the RAAS, importantly implicated in COVID-19 pathology. RAAS mediates key events of the disease, including viral entry in the cell [112, 113] , inflammation, and lung fibrosis [114] . The pressor axis of RAAS bases on Ang II actions through the activation of AT 1 R. ACE is responsible for the cleavage of angiotensin I into Ang II, and Ang II levels are counterbalanced by the depressor axis that degrades Ang II into Ang 1,7 through ACE2 activity. Finally, Ang 1,7 activates MasR [115] . ACE2, independent from its enzymatic activity, serves as SARS-CoV-2 entry route in human cells, but as this enzyme is internalized following virus binding, its enzyme function is lost [6, 116] . Consequently, Ang 1,7 that would exert beneficial effects against CNS damage and neurological deficits [115] is not formed, prevailing Ang II actions mediated by the AT 1 R promoting fibrosis, lung injury, and importantly neuroinflammation [115] .

In fact, the balance between the pressor and depressor axis of RAAS is implicated in neurodegenerative and psychiatric disorders, as carefully reviewed elsewhere [115, 117] . Briefly, components of the depressor axis correlate to enhanced cognition and cell survival in the brain, orchestrating antioxidant, and anti-inflammatory responses. In agreement, inhibition of the pressor axis attenuates cognitive deficits observed in aging, Alzheimer's disease, Parkinson's disease, vascular cognitive impairment and poststroke cognitive impairment [117] .

Since the P2X7 receptor is also involved in lung damage [87] [88] [89] , neurodegenerative and psychiatric disorders [92, 95, 118] , the hypothesis is reasonable that inhibition of this receptor might decrease pathological traits mediated by the RAAS pressor axis and protect against brain and lung injury in COVID-19 patients. Indeed, evidence demonstrates that P2X7 receptor blockade prevents Ang IItriggered pro-inflammatory responses [119] . Furthermore, in rats with diabetic nephropathy, P2X7 receptor expression decreased ACE activity and Ang II levels [120] , which might increase ACE2 expression [43] and counteract SARS-CoV-2-induced inflammatory exacerbation.

Conciliating all these findings, we highlight that the blockade of P2X7 receptors might prevent inflammatory exacerbation both, through its direct actions in inflammasome assembly and through RAAS modulation, avoiding deleterious actions of RAAS in several tissues, including the lung and the brain.

The BBB protects the nervous tissue from direct contact to the blood. Endothelial cells comprise the BBB and are highly selective in consequently filtering the content that is available to neurons [121] . Endothelial cells express high levels of ACE2, which is the main mediator of SARS-CoV-2 infection. The infection and death of these cells may disrupt the barrier and let both the virus and inflammation molecules to access the nervous system (Fig. 1C) .

As previously mentioned, ATP acts as a DAMP and activates the P2X7 receptor, which is largely expressed by endothelial cells and astrocytes/microglial cells, releasing pro-inflammatory cytokines and amplifying the immune response [122] . The opening of P2X7 receptor channels leads to IL-1β production mainly through NLRP3 inflammasome activation [123] , thus contributing to the disruption of the BBB. In this way, SARS-CoV-2 infection can disrupt the BBB by directly infecting and killing endothelial cells, as well as by triggering P2X7 receptor signaling. In agreement, P2X7 receptor antagonism protects against BBB disruption during intracerebral hemorrhage [124] . Moreover, disruption of the BBB due to other viral infections has already been proven to trigger long-term development of neurological disorders, such as Alzheimer's disease, depression, anxiety and multiple sclerosis [125] [126] [127] .

Both intracerebral hemorrhage, against which the P2X7 receptor antagonist protects [124] , and clots are responsible for the primary stroke in the brain [128] . COVID-19 patients are suffering acute cerebrovascular disorder and other correlated neurological symptoms as consequences of stroke or micro-strokes [24] . Stroke includes medical conditions that affect blood vessels of the brain, impairing cerebral circulation as consequence of damaged or deformed arteries ( Fig. 2A) . Diverse hospitals reported increasing levels of patients affected by stroke during SARS-CoV-2 infection [129, 130] . Klok and colleagues observed disturbing increases in the incidence of thrombotic complications in critically ill intensive care unit patients as high as 31% and recommended pharmacological prophylaxis against thrombosis [24] .

The most recent hypothesis for the increased levels of strokes in COVID-19 patients is that hundreds of clots produced in the lungs due to excessive inflammation process may be transported into brain arteries, causing stroke by ischemia. Further, the virus can access the brain through the blood stream and infect BBB cells, as observed for other viruses [131] . Both ways can lead to increased inflammation due to hypoxia, increased acidification and release of DAMPs at the niche of the injury, disrupting the BBB and damaging the CNS and, thus, leading to the development of neuropsychiatric disorders.

As already mentioned, inflammation is mediated by cytokines, chemokines, ROS and other bioactive molecules. These molecules act like DAMPs, signaling astro-and microgliosis. The first immune response to an insult in the nervous system is the activation of microglial cells of the M2 phenotype, which is involved in anti-inflammatory mechanisms for defense and injury repair [132] . However, the sustained activation of these cells induces the phenotypic change to the M1 type, involved in pro-apoptotic processes, production and release of cytokines and ROS that could induce neurodegeneration. In fact, neuroinflammation is closely related to neurodegenerative diseases, as Huntington's [133] , Alzheimer's [134] and Parkinson's disease [135] and neuropsychiatric disorders, such as major depressive disorder, bipolar disorder and anxiety [136] . Since the CNS immune response is also modulated by peripheral components [137] , we hypothesized that the cytokine storm induced by viral infection could stimulate neuroinflammation and neuronal death, facilitating the development of these diseases (Fig. 2B) . Pathological inflammation may be induced by P2X7 receptor activation, and its antagonism has been proposed as a therapeutic approach for COVID-19 treatment [106] .

The role of the P2X7 receptor in neuroinflammatory processes in neuropsychiatric and neurodegenerative diseases is widely studied [95, 118] . In neurodegenerative diseases, cell death is accompanied by neuroinflammatory processes and massive ATP release [138] . In animal models for neurodegeneration P2X7 receptor antagonism exerted beneficial anti-inflammatory effects [138, 139] , indicating that P2X7 receptor activation and subsequent neuroinflammation is tightly related to neurodegeneration worsening. Experimental evidence supports that viral infection may evoke neuronal death, encephalopathy, myelin destruction and juvenile Parkinson's disease development; however, these studies did not include coronaviruses [140] . These evidences indicate that viral infection facilitates neurodegenerative disease development, as Alzheimer's disease, Multiple Sclerosis and Parkinson's Disease [140] . In fact, murine coronavirus is used to induce encephalitis in mice with prominent demyelination and axonal damage. Based on observed pathophysiology and disease progression, this has been proposed as an animal model of multiple sclerosis [141] .

Curiously, anosmia is one of the neurological symptoms reported by SARS-CoV-2 patients. It is worthwhile noting that olfactory impairment is an early predictor of Parkinson's disease development due to α-synuclein aggregation in the olfactory bulb [142] . Although these symptoms might not be disease-related, studies show that α-synuclein induces mitochondrial dysfunction and microglial activation caused by P2X7 receptor stimulation [143, 144] . Further investigation of neural cell marker expression of COVID-19 patients could clarify, whether neuroinflammation is related to the observed anosmia.

Neuroinflammation and increased levels of ATP are observed in neuropsychiatry disorders, supposedly correlated with P2X7 receptor activation [118, 145] . Corroborating this hypothesis, antidepressant treatment decreased P2X7 receptor expression in the ventral hippocampus of stressed animals [146] . Moreover, the treatment with a selective P2X7 receptor antagonist (A-804598) induced antidepressant-like effect associated with inflammasome stimulation [147] and BDNF signaling activation [148] in rodent hippocampus. In this context, we propose that the cytokine storm and cell death induced by SARS-CoV-2 infection results in P2X7 receptor-mediated neuroinflammation, leading to the development of neuropsychiatry disorders. Noteworthy, the P2X7 receptor antagonist JNJ-54175446 is undergoing clinical trials with treatment-resistant depressive patients (EudraCT number 2018-001884-21 at www.clinicaltrialsregister.eu).

As mentioned, a study with 714 patients with COVID-19 found a prevalence of self-reported post-traumatic stress disorder symptoms in 96.2% of them [149] . Another study with 114 patients reported that 34.7% of patients presented anxiety symptoms, while 28.5% of them displayed depression symptoms [150] . Moreover, COVID-19 related psychiatric symptoms (including anger, anxiety, suicidal ideas, hallucinations, insomnia, impaired memory, poor concentration, time disorientation, fear/panic, pressured speech, mood alterations, pessimistic thinking, crying spell and persecutory ideas) have been largely described during and after the occurrence of other respiratory pandemics, such as MERS [151] and SARS [152] [153] [154] [155] [156] [157] [158] [159] . Further indication for psychiatric implications of coronaviruses infections came from a study of Okusaga et al. [160] , who connected seropositivity for human coronavirus strain L63 with mood disorders and suicide attempts. The study could come to a limited conclusion, as it would need to be conducted with more control and coronavirus-serum positive volunteers. However, the authors of this study distinguished between two possible scenarios, which might be important for the understanding of neuronal effects of COVID-19: (1) the connection between depression and immune responses with possible pro-inflammatory interleukin overshooting, which may trigger oxidative stress and neuroinflammation as part of the causes of psychiatric disorders; and (2) viral infection acting as a form of stress. Dysregulated stress and neuroinflammatory responses might cause hypothalamic-pituitary-adrenal (HPA) axis dysfunction. Alterations in the HPA axis have been linked to mood diseases, and HPA activation has been connected with suicide. Hiroi et al. found hyperplastic adrenals in suicide victims, corroborating with such hypothesis [161] . In line, a small COVID-19 patients' study concluded that preexisting psychiatric disease patients reported mental symptom worsening following infection with the virus [162] . Noteworthy, gene knockout or pharmacological inhibition of P2X7 receptors induced antidepressant-like behavior in mice exposed to stress, accompanied by HPA axis restoration [163, 164] . This axis is also activated for counteracting tissue damage, evoked by cytokine storms, in SARS-CoV-2 infection, as previously reviewed [165] . Thus, the HPA axis might provide a connection between coronaviruses and psychiatric disorders. Noteworthy, such mechanism would explain the possible propensity for the development of mood disorders triggered by COVID-19 infection as well as raise the hypothesis that mood disorder patients might be more prone to severe COVID-19 disease development. These patients already carry augmented inflammation patterns, such as systemic inflammation, or suffer from a cytokine storm [166] and show enhanced P2X7 receptor expression, as previously discussed. However, a wide variety of responses to SARS-CoV-2 infection are not yet understood. Further studies are needed to elucidate genetic and environmental factors that could affect individual vulnerability to COVID-19 infection.

Finally, increasing VEGF concentrations, which recruit inflammatory cells into the brain and sustain neuroinflammation, have been named as target for COVID-19 treatment [167] . The COVID-19 entry receptor ACE2 activates RAAS for neuroinflammation and VEGF synthesis by Ang II binding to AT 1 R (reviewed in ref. [167] ). Besides ACE2, aberrant P2X7 receptor activation also induces VEGF release and signaling in the brain [168] . Thus, although VEGF involvement in major depressive disorder is controversially discussed [169, 170] , this growth factor could be involved in the connection between COVID-19, major depressive disorder and purinergic signaling.

Several lines of evidence have raised the possibility of neuroinvasion by SARS-CoV-2 ( Fig. 1) , which may cause short-or long-term impairment of the CNS. The main mechanism involved in this scenario is neuroinflammation, a critical process in psychiatric and neurodegenerative disease development. SARS-CoV-2 infection induces a cytokine storm that could trigger and/or worse neuroinflammatory processes. Patients with mental disorders associated to neuroimmune activation such as depression, Parkinson's or Alzheimer's disease may also present increased susceptibility to SARS-CoV-2 infection and/or severe disease development. Neuroimmune response hyperstimulation observed during viral infection and in mental disorders may be mediated by P2X7 receptor activation (Fig. 2) . In view of that, we suggest P2X7 receptor as a key mediator of the neuroinflammatory process as a possible consequence of SARS-CoV-2 infection. In this context, P2X7 receptor antagonism could be a promising strategy to avoid and treat psychiatric disorders and neurodegenerative diseases of COVID-19 patients.

Structure, function, and antigenicity of the SARS-CoV-2 spike glycoprotein

Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses

SARS-CoV-2 invades host cells via a novel route: CD147-spike protein

CD147 as a target for COVID-19 treatment: suggested effects of azithromycin and stem cell engagement

SARS-CoV-2 cell entry depends on ACE2 and TMPRSS2 and is blocked by a clinically proven protease inhibitor

Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV

Membrane-derived microvesicles: Important and underappreciated mediators of cell-to-cell communication

Platelet-and megakaryocyte-derived microparticles transfer CXCR4 receptor to CXCR4-null cells and make them susceptible to infection by X4-HIV

Cytokine storm intervention in the early stages of COVID-19 pneumonia

Neuropathogenesis and neurologic manifestations of the coronaviruses in the age of coronavirus disease 2019: a review

Multiple organ infection and the pathogenesis of SARS

Magnetic resonance imaging alteration of the brain in a patient with coronavirus disease 2019 (COVID-19) and anosmia

COVID-19-associated acute hemorrhagic necrotizing encephalopathy: CT and MRI features

Neuropathology of COVID-19: a spectrum of vascular and acute disseminated encephalomyelitis (ADEM)-like pathology

Neuropsychiatric sequelae of stroke

The P2X7 receptor in infection and inflammation

ATP-Nlrp3 inflammasome-complement cascade axis in sterile brain inflammation in psychiatric patients and its impact on stem cell trafficking

The Nlrp3 inflammasome as a "rising star" in studies of normal and malignant hematopoiesis

Protein reviews. Advances in experimental medicine and biology. Singapore

The P2X7 receptor: a main player in inflammation

P2X7 receptor: An emerging target in central nervous system diseases

Lifting the mask on neurological manifestations of COVID-19

Incidence of thrombotic complications in critically ill ICU patients with COVID-19

SARS-CoV-2: olfaction, brain infection, and the urgent need for clinical samples allowing earlier virus detection

Multiorgan and renal tropism of SARS-CoV-2

Neuroinvasion of SARS-CoV-2 in human and mouse brain

SARS-CoV-2 infection in the central nervous system of a 1-year-old infant submitted to complete autopsy

Central nervous system involvement by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)

The blood-brain barrier and the role of cytokines in neuropsychiatry

Cytokine signaling modulates blood-brain barrier function

Viral disruption of the blood-brain barrier

The neuroinvasive potential of SARS-CoV2 may play a role in the respiratory failure of COVID-19 patients

SARS-CoV-2 receptor and entry genes are expressed by sustentacular cells in the human olfactory neuroepithelium

Non-neuronal expression of SARS-CoV-2 entry genes in the olfactory system suggests mechanisms underlying COVID-19-associated anosmia

Lethal infection of K18-hACE2 mice infected with severe acute respiratory syndrome coronavirus

Vacuolating encephalitis in mice infected by human coronavirus OC43

Middle east respiratory syndrome coronavirus causes multiple organ damage and lethal disease in D

mice transgenic for human dipeptidyl peptidase 4

Pathological findings of COVID-19 associated with acute respiratory distress syndrome

Role of chemokines in the pathogenesis of acute lung injury

Complement and its role in innate and adaptive immune responses

The NLRP3 inflammasome: molecular activation and regulation to therapeutics

IL-1alpha, IL-33 and S100 proteins: dual-function alarmins

Interactions of coronaviruses with ACE2, angiotensin II, and RAS inhibitors-lessons from available evidence and insights into COVID-19

The P2X7 receptor primes IL-1beta and the NLRP3 inflammasome in astrocytes exposed to mechanical strain

Covid-19 and hypercoagulable state: a new therapeutic perspective

Sterile inflammation of brain, due to activation of innate immunity, as a culprit in psychiatric disorders

P2X receptors in health and disease

ATP-gated P2X7 receptors require chloride channels to promote inflammation in human macrophages

Identification of two novel markers for alveolar epithelial type I and II cells

Re-evaluation of neuronal P2X7 expression using novel mouse models and a P2X7-specific nanobody

Genetically dissecting P2rx7 expression within the central nervous system using conditional humanized mice

The role of microglial P2X7: modulation of cell death and cytokine release

Involvement of P2X7 receptors in the regulation of neurotransmitter release in the rat hippocampus

Supersensitivity of P2X7 receptors in cerebrocortical cell cultures after in vitro ischemia

Cellular localization of P2X7 receptor mRNA in the rat brain

Emerging challenges of assigning P2X7 receptor function and immunoreactivity in neurons

Neuronal P2X7 receptors revisited: do they really exist?

Astrocytic rather than neuronal P2X7 receptors modulate the function of the trisynaptic network in the rodent hippocampus

Increase of intracellular Ca2+ by P2X and P2Y receptorsubtypes in cultured cortical astroglia of the rat

Functional P2X7 receptors at cultured hippocampal astrocytes but not neurons

SARS-CoV-2 infection and overactivation of Nlrp3 inflammasome as a trigger of cytokine "storm" and risk factor for damage of hematopoietic stem cells

Properties of the poreforming P2X7 purinoceptor in mouse NTW8 microglial cells

Effects of antagonists at the human recombinant P2X7 receptor

The elusive P2X7 macropore

P2X7 interactions and signaling-making head or tail of it

Significance of p2x7 receptor variants to human health and disease

Detecting adenosine triphosphate in the pericellular space

Increased level of extracellular ATP at tumor sites: in vivo imaging with plasma membrane luciferase

Graft-versus-host disease is enhanced by extracellular ATP activating P2X7R

P2X7 receptor-stimulation causes fever via PGE2 and IL-1β release

Molecular mechanisms regulating NLRP3 inflammasome activation

NLRP3 inflammasome is expressed and functional in mouse brain microglia but not in astrocytes

Emerging role of the P2X7-NLRP3-IL1β pathway in mood disorders

Human monocyte stimulus-coupled IL-1β posttranslational processing: modulation via monovalent cations

Nonclassical IL-1 beta secretion stimulated by P2X7 receptors is dependent on inflammasome activation and correlated with exosome release in murine macrophages

Astrocyte-derived ATP induces vesicle shedding and IL-1β release from microglia

Activation of P2X7 receptors induces CCL3 production in microglial cells through transcription factor NFAT

Production and release of neuroprotective tumor necrosis factor by P2X 7 receptor-activated microglia

P2X7-dependent, but differentially regulated release of IL-6, CCL2, and TNF-α in cultured mouse microglia

P2X7 receptor activation induces CXCL2 production in microglia through NFAT and PKC/MAPK pathways

The role of p2x7 receptor in infectious inflammatory diseases and the influence of ectonucleotidases

The P2X7 receptor in inflammatory diseases: angel or demon?

P2X7 receptor induces mitochondrial failure in monocytes and compromises NLRP3 inflammasome activation during sepsis

P2X7 receptor signaling contributes to sepsis-associated brain dysfunction

Disruption of the P2X7 purinoceptor gene abolishes chronic inflammatory and neuropathic pain

Autocrine regulation of T-cell activation by ATP release and P2X7 receptors

Deletion of P2X7 attenuates hyperoxia-induced acute lung injury via inflammasome suppression

P2X7 receptor is involved in lung injuries induced by ischemiareperfusion in pulmonary arterial hypertension rats

Extracellular ATP is a danger signal activating P2X7 receptor in lung inflammation and fibrosis

Blockage of P2X7 attenuates acute lung injury in mice by inhibiting NLRP3 inflammasome

ATP/P2X7-NLRP3 axis of dendritic cells participates in the regulation of airway inflammation and hyper-responsiveness in asthma by mediating HMGB1 expression and secretion

Purinergic system in psychiatric diseases

The microglial ATP-gated ion channel P2X7 as a CNS drug target

Purinergic signalling and neurological diseases: an update

The P2X7 receptor: central hub of brain diseases

Into the eye of the cytokine storm

The pathogenesis and treatment of the 'Cytokine Storm" in COVID-19

Clinical features of patients infected with 2019 novel coronavirus in Wuhan

Plasma IP-10 and MCP-3 levels are highly associated with disease severity and predict the progression of COVID-19

SARS-CoV-2: a storm is raging

Clinical and immunological features of severe and moderate coronavirus disease 2019

Dysregulation of immune response in patients With COVID-19 in Wuhan, China

Diagnostic utility of clinical laboratory data determinations for patients with the severe COVID-19

The role of interleukin-6 in monitoring severe case of coronavirus disease 2019

Clinical features of 69 cases with coronavirus disease 2019 in Wuhan, China

A rationale for targeting the P2X7 receptor in Coronavirus disease 19 (Covid-19)

P2X7 receptor modulates inflammatory and functional pulmonary changes induced by silica

Virus-triggered ATP release limits viral replication through facilitating IFN-β production in a P2X7-dependent manner

Extracellular ATP induces the rapid release of HIV-1 from virus containing compartments of human macrophages

Contribution of the purinergic receptor P2X7 to development of lung immunopathology during influenza virus infection

Activation of P2X(7) receptor by ATP plays an important role in regulating inflammatory responses during acute viral infection

Angiotensin-converting enzyme 2 (ACE2) as a SARS-CoV-2 receptor: molecular mechanisms and potential therapeutic target

Insights in chloroquine action: perspectives and implications in Malaria and COVID-19

Chronic activation of the renin-angiotensin system induces lung fibrosis

The depressor axis of the renin-angiotensin system and brain disorders: A translational approach

SARS-CoV-2 receptor and regulator of the renin-angiotensin system: celebrating the 20th anniversary of the discovery of ACE2

Within the brain: the renin angiotensin system

P2X7 receptor signaling in stress and depression

The role of P2X7 purinergic receptors in the renal inflammation associated with angiotensin II-induced hypertension

Inhibition of the P2X7 receptor improves renal function via renin-angiotensin system and nitric oxide on diabetic nephropathy in rats

The blood-brain barrier

ATP induces disruption of tight junction proteins via IL-1 beta-dependent MMP-9 activation of human blood-brain barrier in vitro

The P2X7 receptor-interleukin-1 liaison

P2X7 receptor suppression preserves blood-brain barrier through inhibiting RhoA activation after experimental intracerebral hemorrhage in rats

Role of the blood-brain barrier in multiple sclerosis

Blood-brain barrier dysfunction as a cause and consequence of Alzheimer's disease

Molecular adaptations of the blood-brain barrier promote stress resilience vs

COVID-19 presenting as stroke

Impact of COVID-19 pandemic on critical care transfers for ST-elevation myocardial infarction, stroke, and aortic emergencies

Potential mechanisms of hemorrhagic stroke in elderly COVID-19 patients

Vascular disruption and blood-brain barrier dysfunction in intracerebral hemorrhage

Differential Roles of M1 and M2 microglia in neurodegenerative diseases

Neuroinflammation in Huntington's disease: new Insights with 11C-PBR28 PET/MRI

Neuroinflammatory processes in Alzheimer's disease

Outside in: unraveling the role of neuroinflammation in the progression of Parkinson's disease

Modulating neuroinflammation to treat neuropsychiatric disorders

Systemic inflammation and the brain: novel roles of genetic, molecular, and environmental cues as drivers of neurodegeneration

Purinergic receptors in neurological diseases with motor symptoms: targets for therapy

P2Y6 and P2X7 receptor antagonism exerts neuroprotective/ neuroregenerative effects in an animal model of Parkinson's disease

Viruses and neurodegeneration

Malykhina AP. Differential neurodegenerative phenotypes are associated with heterogeneous voiding dysfunction in a coronavirus-induced model of multiple sclerosis

The olfactory bulb as the entry site for prion-like propagation in neurodegenerative diseases

P2X7 receptor-pannexin 1 interaction mediates extracellular alpha-synuclein-induced ATP release in neuroblastoma SH-SY5Y cells

P2X7 receptor is critical in α-synuclein-mediated microglial NADPH oxidase activation

P2X7 receptor: a potential therapeutic target for depression?

Reduced P2X receptor levels are associated with antidepressant effect in the learned helplessness model

Psychological stress activates the inflammasome via release of adenosine triphosphate and stimulation of the purinergic type 2X7 receptor

Antidepressant-like effect induced by P2X7 receptor blockade in FSL rats is associated with BDNF signalling activation

Posttraumatic stress symptoms and attitude toward crisis mental health services among clinically stable patients with COVID-19 in China

Prevalence and Factors Associated with Depression and Anxiety of Hospitalized Patients with COVID-19

Psychiatric findings in suspected and confirmed middle east respiratory syndrome patients quarantined in hospital: a retrospective chart analysis

Psychiatric complications in patients with severe acute respiratory syndrome (SARS) during the acute treatment phase: a series of 10 cases

Stress and psychological impact on SARS patients during the outbreak

Stress and psychological distress among SARS survivors 1 year after the outbreak

The effects of disease severity, use of corticosteroids and social factors on neuropsychiatric complaints in severe acute respiratory syndrome (SARS) patients at acute and convalescent phases

Psychological distress and negative appraisals in survivors of severe acute respiratory syndrome (SARS)

Long-term psychiatric morbidities among SARS survivors

Posttraumatic stress disorder in convalescent severe acute respiratory syndrome patients: a 4-year follow-up study

Mental morbidities and chronic fatigue in severe acute respiratory syndrome survivors long-term follow-up

Association of seropositivity for influenza and coronaviruses with history of mood disorders and suicide attempts

Expression of corticotropin releasing hormone receptors type I and type II mRNA in suicide victims and controls

COVID-19 pandemic and mental health consequences: systematic review of the current evidence

A P2X7 receptor antagonist reverses behavioural alterations, microglial activation and neuroendocrine dysregulation in an unpredictable chronic mild stress (UCMS) model of depression in mice

The absence of P2X7 receptors (P2rx7) on nonhaematopoietic cells leads to selective alteration in mood-related behaviour with dysregulated gene expression and stress reactivity in mice

Psycho-Neuroendocrine-Immune Interactions in COVID-19: potential impacts on mental health

Danger signals and inflammasomes: stress-evoked sterile inflammation in mood disorders

Vascular endothelial growth factor (VEGF) as a vital target for brain inflammation during the COVID-19 outbreak

Pleiotropic roles of P2X7 in the central nervous system

Vascular endothelial growth factor: a potential diagnostic biomarker for major depression

Vascular endothelial growth factor (VEGF) and its role in the central nervous system: a new element in the neurotrophic hypothesis of antidepressant drug action

SARS-CoV-2 receptor ACE2 is an interferon-stimulated gene in human airway epithelial cells and is detected in specific cell subsets across tissues

Alveolar type I cells protect rat lung epithelium from oxidative injury

Cerebroprotective action of angiotensin peptides in stroke

Purinergic signaling in the vertebrate olfactory system

Expression of emmprin (CD147), a cell surface inducer of matrix metalloproteinases, in normal human brain and gliomas

Distribution of ACE2, CD147, CD26 and other SARS-CoV-2 associated molecules in tissues and immune cells in health and in asthma, COPD, obesity, hypertension, and COVID-19 risk factors

Is cholesterol and amyloid-β stress induced CD147 expression a protective response? Evidence that extracellular cyclophilin a mediated neuroprotection is reliant on CD147

Prevalence of psychological morbidities among general population, healthcare workers and COVID-19 patients amidst the COVID-19 pandemic: a systematic review and meta-analysis

Impact of COVID-19 pandemic on mental health in the general population: a systematic review

Psychological effects caused by the COVID-19 pandemic in health professionals: a systematic review with meta-analysis

Prevalence of stress, anxiety, depression among the general population during the COVID-19 pandemic: a systematic review and meta-analysis

Impact of coronavirus syndromes on physical and mental health of health care workers: systematic review and meta-analysis

The psychological and mental impact of coronavirus disease 2019 (COVID-19) on medical staff and general public-a systematic review and meta-analysis

Prevalence of depression, anxiety, and insomnia among healthcare workers during the COVID-19 pandemic: a systematic review and meta-analysis