key: cord-1049962-6jfv5dt6 authors: Moss, William J.; Griffin, Diane E. title: Global measles elimination date: 2006-11-06 journal: Nat Rev Microbiol DOI: 10.1038/nrmicro1550 sha: 50907351f259a394edec8562044c15d7e5928080 doc_id: 1049962 cord_uid: 6jfv5dt6 Measles remains a leading vaccine-preventable cause of child mortality worldwide, particularly in sub-Saharan Africa where almost half of the estimated 454,000 measles deaths in 2004 occurred. However, great progress in measles control has been made in resource-poor countries through accelerated measles-control efforts. The global elimination of measles has been debated since measles vaccines were first licensed in the 1960's, and this debate is likely to be renewed if polio virus is eradicated. This review discusses the pathogenesis of measles and the likelihood of the worldwide elimination of this disease. Measles, which is caused by the measles virus (MV) (BOX 1) , was estimated to cause 454,000 deaths in 2004, almost half of which were in sub-Saharan Africa, and continues to cause outbreaks in communities with low vaccination coverage in industrialized nations 1 (FIG. 1) . It is one of the most important infectious diseases of humans and has caused millions of deaths since its emergence thousands of years ago. The disease is characterized by a prodromal illness of fever, cough, coryza and conjunctivitis followed by the appearance of a generalized maculopapular rash. Deaths from measles are mainly due to an increased susceptibility to secondary bacterial and viral infections, which is attributed to a prolonged state of MV-induced immune suppression (BOX 2) . MV most closely resembles rinderpest virus, a pathogen of cattle, and might have evolved as a zoonotic infection from an ancestral virus that was present in communities in which cattle and humans lived in close proximity. MV is thought to have become established in human populations approximately 5,000 years ago, when human populations achieved sufficient size in the Middle Eastern river valley civilizations to maintain virus transmission. The virus was introduced into the Americas in the sixteenth century as a result of European exploration of the New World, resulting in thousands of deaths in native Amerindian populations. Deaths in this susceptible native American population due to measles and smallpox facilitated the European conquest of the Americas 2 . MV was first isolated from the blood of David Edmonston in 1954 by John Enders and Thomas Peebles 3 . The development of vaccines against measles soon followed 4 . Respiratory droplets from infected persons function as vehicles of transmission by delivering infectious virus to epithelial cells of the respiratory tract of susceptible hosts. During the 10-14 day incubation period between infection and the onset of clinical signs and symptoms, MV replicates and spreads in the infected host (FIG. 2) . Initial viral replication occurs in epithelial cells at the entry site in the upper respiratory tract, and the virus then spreads to local lymphatic tissue. Replication in local lymph nodes is followed by viraemia and the dissemination of MV to many organs, including lymph nodes, skin, kidney, gastrointestinal tract and liver, in which the virus replicates in the epithelial and endothelial cells, and in lymphocytes, monocytes and macrophages. Host immune responses (FIG. 2) to MV are essential for viral clearance, clinical recovery and the establishment of long-term immunity. Early innate immune responses occur during the prodromal phase and include activation of natural killer (NK) cells and increased production of interferons (IFN)-α and β. The adaptive immune responses consist of MV-specific humoral and cellular responses. The protective efficacy of antibodies to MV is shown by the immunity conferred to infants from passively acquired maternal antibodies and the protection of exposed, susceptible individuals following post-exposure administration of anti-MV immune globulin 5 . The most abundant and rapidly produced antibodies are against the nucleoprotein (N). Antibodies to the haemagglutinin (H) and fusion (F) proteins contribute to virus neutralization and are sufficient to provide protection. Evidence for the importance of cellular immunity to MV is demonstrated by the ability of children with agammaglobulinaemia to fully recover from measles, whereas children with severe defects in T-lymphocyte function often develop severe or fatal disease 6 . Monkeys depleted of CD8 + T-lymphocytes and challenged with wild-type MV had a more extensive rash, higher levels of MV in the blood and a longer duration of viraemia than control animals 7 . CD4 + T-lymphocytes are activated in An antigen to which a host has previously been exposed. response to MV infection and secrete cytokines capable of directing humoral and cellular immune responses. Plasma cytokine profiles show increased levels of IFN-γ during the acute phase, followed by a shift to high levels of interleukin (IL)-4 and IL-10 during convalescence 8 . The initial predominant T-helper-1 (Th1)-response is essential for viral clearance, and the later Th2 response promotes the development of protective MV-specific antibodies. The immune responses induced by MV infection are paradoxically associated with depressed responses to non-MV antigens, and this effect continues for several weeks to months after resolution of the acute illness. Following MV infection, delayed-type hypersensitivity (DTH) responses to recall antigens, such as tuberculin, are suppressed 9 and cellular and humoral responses to new antigens are impaired 10 . This MV-induced immune suppression renders individuals more susceptible to secondary bacterial and viral infections that can cause pneumonia and diarrhoea, and is responsible for much of the measles-related morbidity and mortality 11, 12 . Pneumonia, the most common fatal complication of measles, occurs in 56-86% of measles-related deaths 13 . Abnormalities of both the innate and adaptive The measles virus (MV) is a spherical, non-segmented, single-stranded, negative sense RNA virus (see the figure part a) and is a member of the Morbillivirus genus in the family of Paramyxoviridae. Other members of the Morbillivirus genus that are not pathogenic to humans include rinderpest virus and canine distemper virus. MV is killed by ultraviolet light and heat. Attenuated measles vaccine viruses retain these characteristics, necessitating a cold chain for the transport and storage of measles vaccines. The MV RNA genome comprises approximately 16,000 nucleotides (see the figure part b) and is enclosed in a lipidcontaining envelope that is derived from the host cell. The genome encodes eight proteins, two of which (V and C) are non-structural proteins and are alternatively translated from the RNA, or an edited RNA, coding for the phosphoprotein (P). Of the six structural proteins, P, large protein (L) and nucleoprotein (N) form the nucleocapsid that encloses the viral RNA. The haemagglutinin protein (H), fusion protein (F) and matrix protein (M), together with lipids from the host cell membrane, form the viral envelope. The H protein interacts with F to mediate attachment and fusion of the viral envelope with the host cell membrane, enabling viral entry into the cell 80 (see the figure part c). The primary function of the H protein is to bind to the host cellular receptors for MV. The two identified receptors are CD46 and CD150 (also known as SLAM). CD46 is a complement regulatory molecule expressed on all nucleated cells in humans. SLAM (signalling lymphocyte activation molecule) is expressed on activated T and B lymphocytes and antigen-presenting cells. The binding sites on H for these receptors overlap and strains of MV differ in the efficiency with which each receptor is used. Wild-type MV binds to cells primarily through the cellular receptor SLAM, whereas most vaccine strains bind to CD46, as well as to SLAM 81 . Other unidentified receptors for MV probably exist on human endothelial and epithelial cells 82 . Remaining MV proteins are involved in viral replication. The P protein regulates transcription, replication and the efficiency with which the nucleoprotein assembles into nucleocapsids 83 . The M protein links ribonucleoproteins with envelope proteins during virion assembly. The functions of the V and C proteins have not been clearly defined, but both proteins seem to contribute to the virulence of MV by regulating transcription and sensitivity to the antiviral effects of immune responses have been described following MV infection. Transient lymphopaenia with a reduction in CD4 + and CD8 + T-lymphocytes occurs in children with measles. Functional abnormalities of immune cells have also been detected, including decreased lymphocyte proliferative responses 14 . Dendritic cells that are infected with MV in vitro mature poorly, lose the ability to stimulate responses in lymphocytes and undergo cell death 15 . The dominant Th2-response in children recovering from measles can inhibit Th1 responses and increase susceptibility to intracellular pathogens 16, 17 . The production of IL-12, which is important for the generation of the Th1-type immune response, is decreased in vitro following binding of CD46 (REF. 18 ), an MV receptor, and is reduced for several weeks in children with measles 19 . Conversely, IL-10 production is elevated for several weeks in the plasma of children with measles 8 . IL-10 downregulates the synthesis of cytokines, suppresses macrophage activation and T-cell proliferation and inhibits DTH responses. Prior to the development and widespread use of measles vaccines, measles was estimated to result in 5-8 million deaths annually. The decline in mortality from measles in developed countries was associated with economic development, improved nutritional status and supportive care, and antibiotic therapy for secondary bacterial pneumonia. Remarkable progress in reducing measles incidence and mortality has been made in resourcepoor countries, particularly in sub-Saharan Africa 20 , as a consequence of increasing measles vaccine coverage, provision of a second opportunity for measles vaccination through supplementary immunization activities, and efforts by the WHO, the United Nations Children's Fund (UNICEF) and their partners to target 45 countries for accelerated and sustained measles mortality reduction 21 . Provision of vitamin A through polio and measles vaccination campaigns has contributed further to the reduction in measles mortality 1 The global elimination of measles has been debated since the 1960's, shortly after measles vaccines were first licensed 23 . The Dahlem Conference on Disease Eradication (1997) defined eradication as the permanent reduction to zero of the global incidence of infection caused by a specific pathogen as a result of deliberate efforts, with the consequence that interventions would no longer be necessary. Although modifications to this definition were subsequently proposed, three criteria were deemed necessary for a disease to be considered eradicable: first, humans must be crucial for transmission; second, sensitive and specific diagnostic tools must exist; and finally, an effective intervention must be available. Demonstration of the interruption of transmission in a large geographic area for a prolonged period further supports the feasibility of eradication. Measles is thought by many experts to meet these criteria for eradication 24, 25 . Biological feasibility of measles elimination MV has no non-human reservoirs, can be readily diagnosed after the onset of a rash and has not mutated or evolved to significantly alter immunogenic epitopes. However, MV is highly infectious, requiring a high level of population immunity to interrupt transmission, is contagious for several days prior to the onset of the rash (the first easily diagnosed symptom), and might be more difficult to eliminate in regions that have a high prevalence of human immunodeficiency virus type 1 (HIV-1). 27 . Subclinical measles is defined as a fourfold rise in MV-specific IgG antibodies following exposure to wildtype MV in an asymptomatic individual with some prior measles immunity. Subclinical infection might be important in boosting protective antibody levels in children with decreasing immunity 28 , but raises the concern that persons with incomplete immunity and subclinical infection might be capable of transmitting MV 29 . MV has been isolated from a naturally immune, asymptomatically re-infected individual 30 , and extensive epidemiological investigation of a person with measles in the Netherlands failed to identify a contact with clinically apparent measles, implying that transmission might have occurred from a person with subclinical infection 31 . Although RNA viruses have high mutation rates 32 , MV is an antigenically monotypic virus: the surface proteins that are responsible for inducing protective immunity have retained their antigenic structure across time and space. The public health significance of this is that measles vaccines developed decades ago from a single MV strain remain protective worldwide. However, variability in the genome is sufficient to allow for molecular epidemiologic investigation. One of the most variable regions of the MV genome is the 450-nucleotide sequence at the carboxyl terminus of the N protein, with up to 12% variability between wild-type viruses. The WHO recognizes 8 clades of MV (designated A to H) and 23 genotypes 33, 34 . New genotypes will probably be identified with improved surveillance and molecular characterization. As measles-control efforts intensify, molecular surveillance of circulating MV strains could be used to document the interruption of MV transmission and to identify the source and transmission pathways of MV outbreaks 35, 36 . Clinically apparent measles begins with a prodrome characterized by fever, cough, coryza (runny nose) and conjunctivitis (FIG. 2) . Koplik's spots, which are small white lesions on the buccal mucosa inside the mouth, might be visible during the prodrome. The prodromal symptoms intensify several days before the onset of rash. The characteristic erythematous and maculopapular rash appears first on the face and behind the ears, and then spreads to the trunk and the extremities. The rash lasts for 3-4 days after which it fades, disappearing from the face first. In uncomplicated measles, clinical recovery begins soon after the appearance of the rash. The risk of complication is increased by extremes of age, malnutrition and vitamin A deficiency. Complications of measles have been described in almost every organ system. Pneumonia accounts for most of the approximately 500,000 measles-associated deaths annually, and is caused by secondary viral or bacterial infections or by MV itself. Other respiratory complications include laryngotracheobronchitis (croup) and otitis media (ear infection). Mouth ulcers, or stomatitis, might hinder children from eating or drinking. Many children with measles develop diarrhoea, further contributing to malnutrition. Eye disease (keratoconjunctivitis) is common after measles, particularly in children with vitamin A deficiency, and can cause blindness. Rare but serious complications of measles involve the central nervous system (CNS). Post-measles encephalomyelitis, an autoimmune disorder triggered by MV infection, occurs in approximately 1 in 1000 cases, and is mainly confined to older children and adults. Encephalomyelitis occurs within two weeks of the onset of rash and is characterized by fever, seizures and various neurological abnormalities. Other CNS complications that occur months to years after acute infection are measles inclusion body encephalitis (MIBE) and subacute sclerosing panencephalitis (SSPE). MIBE and SSPE are caused by persistent MV infection. MIBE is a rare but fatal complication that affects individuals with defective cellular immunity and typically occurs months after infection. SSPE is a slow progressive disease characterized by seizures, progressive deterioration of cognitive and motor functions and by death, which occurs 5-15 years after MV infection, most often in persons infected with MV before 2, which years of age. Infectiousness. MV is one of the most highly contagious infectious agents and outbreaks can occur in populations in which less than 10% of individuals are susceptible. The contagiousness of MV is best expressed by the basic reproductive number (R o ), which is the mean number of secondary cases that would arise if an infectious agent were introduced into a completely susceptible population. R o is a function not only of the infectious agent but also of the host population. The estimated R o for MV is generally assumed to be 12-18, in contrast to only 5-7 for smallpox virus and 2-3 for SARS coronavirus. In the 1951 measles epidemic in Greenland, the index case attended a community dance during the infectious period resulting in an R o of 200 (REF. 37 ). The high infectivity of MV implies that a high level of population immunity (approximately 95%) is required to interrupt MV transmission. Further hindering elimination efforts is the fact that persons with measles are infectious during the prodromal phase, several days prior to the onset of rash, and therefore transmit the virus prior to clinical case detection. MV can be isolated in tissue culture from urine as late as one week after the onset of rash. Detection of MV in body fluids by various means, including identification of multinucleated giant cells in nasal secretions and the use of PCR after reverse transcription of RNA (RT-PCR), indicates the potential for prolonged infectious periods in persons immunocompromised by severe malnutrition or HIV-1 infection 38, 39 . However, whether detection of MV by these methods indicates prolonged contagiousness is unclear, and prolonged transmission of MV is not likely to be a significant obstacle to eradication. In contrast to polioviruses 40 , transmission of the live attenuated measles vaccine virus has not been reported. In regions of high HIV-1 prevalence and crowding, such as urban centres in sub-Saharan Africa, HIV-1-infected children could have a role in the sustained transmission of MV 41 . HIV-1-infected mothers have defective transfer of IgG antibodies across the placenta, resulting in lower titres of protective antibodies in the infant, which increases the period of susceptibility to MV infection prior to routine immunization. HIV-1-infected children that are vaccinated against MV might become susceptible to MV owing to progressive HIV-1-induced immunosuppression. Children with defective cell-mediated immunity might not develop the characteristic measles rash, and infection might therefore go unrecognized, with the potential for widespread transmission of MV, particularly in healthcare settings. Finally, HIV-1-infected children might have impaired cell-mediated immune clearance and prolonged shedding of MV 39 , increasing the period of infectivity and the spread of MV to secondary contacts. However, even if HIV-1-infected children lose protective immunity over time, the high mortality rate of HIV-infected children, particularly in sub-Saharan Africa where approximately one third of untreated HIV-1-infected children die by one year of age and half are dead by two years of age, is such that they do not live long enough for a sizeable pool of MV-susceptible children to accumulate 42 . This might change with increased access to antiretroviral drugs. Successful control of measles in the countries of southern Africa suggests that the HIV-1 epidemic is not a significant barrier to measles control 20,43 . The infected person who first introduces an infection into a population. Developmental deficiencies in the immune responses of newborns and infants. Technical feasibility of measles elimination Measles vaccines are safe, effective and have interrupted MV transmission in large geographic areas, providing a crucial tool for global measles elimination. Several attenuated measles vaccines are available worldwide, either as single-virus vaccines or in combination with other vaccine viruses (commonly rubella and mumps). Most of the currently used measles vaccines were derived from the Edmonston strain of MV isolated by Enders and Peebles in 1954. These vaccines have undergone different passage histories in cell culture, but nucleotide sequence analyses show genetic differences of less than 0.6% between most vaccine strains (FIG. 3) . The measles vaccine induces both humoral and cellular immune responses. Antibodies first appear between 12 and 15 days after vaccination and peak at 21 to 28 days. IgG antibodies persist in the blood for years (BOX 2) . Vaccination also induces MV-specific cellular immune responses 44 . The proportion of children who develop protective antibody titres following measles vaccination depends on the presence of inhibitory maternal antibodies and the immunological maturity of the vaccine recipient, as well as the dose and strain of vaccine virus. Polymorphisms in human immune-response genes (for example, TAP2 and HLA-DQA1) also influence immune responses to measles vaccine 45 . Frequently cited statistics are that approximately 85% of children develop protective antibody titres when the measles vaccine is administered at 9 months of age, and 90-95% have a protective antibody response after vaccination at 12 months of age 46 . The duration of protective antibody titres following measles vaccination is more variable and shorter than that acquired through infection with wild-type MV, with an estimated 5% of children losing protective antibody titres 10-15 years after vaccination 47 . However, decreasing antibody titres do not necessarily imply a loss of protective immunity, as a secondary immune response usually develops after re-exposure to MV, with a rapid rise in IgG antibodies. Despite the public health benefits of measles vaccines, there are several limitations of the licensed vaccines that might be important for global measles elimination. First, attenuated measles vaccines are inactivated by light and heat, and lose about half of their potency after reconstitution if stored at 20 o C for one hour and almost all potency if stored at 37 o C for one hour. A cold chain must be maintained to support measles immunization activities. Second, measles vaccines must be injected subcutaneously or intramuscularly, necessitating trained healthcare workers, needles, syringes and the proper disposal of hazardous waste. Third, both maternally acquired antibodies and immunological immaturity reduce the protective efficacy of measles vaccination in early infancy, hindering effective immunization of young infants 48 . Fourth, the attenuated measles vaccine has the potential to cause serious outcomes, such as lung or brain infection, in severely immunocompromised persons 49 . Finally, as discussed below, a second opportunity for measles vaccination, in addition to the first dose through routine immunization services, must be provided to achieve high enough levels of population immunity to interrupt MV transmission. High levels of population immunity against measles, achieved through high measles-vaccine coverage, can interrupt MV transmission. Perhaps the main challenge to global measles-elimination efforts will be to maintain the resources, political will and public confidence to implement intensive measles vaccination and surveillance activities. Measles elimination is the interruption of MV transmission in a defined geographical area. Small outbreaks of primary and secondary cases might still occur following importation from outside the region, but sustained transmission does not occur. Because of the high infectivity of MV and the fact that not all individuals develop protective immunity following vaccination, a single dose of measles vaccine does not achieve a sufficient level of population immunity to eliminate measles. A second opportunity for measles immunization is necessary to eliminate measles by providing protective immunity to children who failed to respond to the first dose and to those who were not previously vaccinated. Two broad strategies to administer the second dose have been used. In countries with sufficient infrastructure, the second dose of measles vaccine is administered through routine immunization services, typically prior to the start of school. High coverage The administration of a drug or vaccine other than through the intestine, usually by injection. levels are ensured by school entry requirements. A second approach, first developed by the Pan American Health Organization (PAHO) for South and Central America 50 , involves mass-immunization campaigns (called supplementary immunization activities, or SIAs) to deliver the second dose of measles vaccine. This strategy was successful in eliminating measles in Latin America, and has resulted in a marked reduction in measles incidence and mortality in parts of sub-Saharan Africa 43 . The PAHO strategy consists of four sub-programmes: Catch-Up, Keep-Up, Follow-Up and Mop-Up 50 . The Catch-Up phase is a one-time, massimmunization campaign that targets all children in a broad age group regardless of whether they previously had wild-type MV infection or measles vaccination. The goal is to rapidly achieve a high level of population immunity and interrupt MV transmission. If successful, SIA activities are cost effective 51, 52 and can abruptly interrupt MV transmission with dramatic declines in incidence and mortality. Keep-Up refers to the need to maintain >90% routine measles vaccine coverage through improved access to measles vaccination and a reduction in missed opportunities. Follow-Up refers to periodic mass campaigns to prevent the accumulation of susceptible children that typically target children 1-4 years of age, a narrower age group than targeted in Catch-Up campaigns. Mop-Up campaigns target children that are difficult to reach in sites of measles outbreaks or low vaccine coverage. Polio vaccination campaigns have been successfully conducted during scheduled ceasefires in regions of conflict 53 . Similar results should be achievable for measles vaccination campaigns, although additional highly skilled healthcare workers are needed to administer parenteral measles vaccine compared with oral poliovirus vaccine. However, maintaining high levels of routine measles vaccine coverage in areas of conflict is extremely difficult, and devastating measles outbreaks frequently occur in refugee populations 54 . The ease of global travel facilitates the importation of MV into regions where measles has been successfully controlled (as exemplified by the 2006 measles outbreak in Boston, USA) and cross-border population movements necessitate regional rather than country level control strategies. Finally, measles control might be particularly difficult in the high-density slum areas of large cities in Africa and Asia, where several factors converge to facilitate MV transmission among susceptible persons, including high population density and difficulties in achieving high vaccination coverage. A loss of public confidence in vaccines can significantly impair elimination efforts, as demonstrated by the poliovirus outbreaks in northern Nigeria that spread across several continents after a loss of public confidence in polio vaccines 55 . Measles outbreaks have occurred in communities that are opposed to vaccination on religious or philosophical grounds 56 . Much public attention has focused on a purported association between the measles-mumps-rubella (MMR) vaccine and autism following the publication of a report in 1998 hypothesizing that the MMR vaccine might cause a syndrome of autism and intestinal inflammation 57 . The events that followed, and the public concern over the safety of the MMR vaccine, led to diminished vaccine coverage in the United Kingdom and provide important lessons in the misinterpretation of epidemiological evidence and the communication of scientific results to the public 58 . As a consequence, measles outbreaks became more frequent and larger in size 59 . Subsequently, several comprehensive reviews and epidemiological studies rejected evidence for a causal relationship between MMR vaccination and autism 60, 61 . New tools for measles eradication Aerosol administration of measles vaccine was first evaluated in the early 1960s in several countries, including the former Soviet Union and the United States. More recent studies in South Africa 62 and Mexico 63 have shown that aerosol administration of measles vaccine is highly effective in boosting antibody titres, although the primary immune response to aerosolized measles vaccine is reduced compared with subcutaneous administration 64 . Administration of measles vaccine by aerosol has the potential to facilitate measles vaccination during mass campaigns, and the WHO plans to test and bring to licensure an aerosol measles vaccine by 2009. The ideal measles vaccine would be inexpensive, safe, heat-stable, immunogenic in neonates or very young infants, and administered as a single dose without the need to use a needle or syringe. The age at vaccination should coincide with the Expanded Programme on Immunization (EPI) schedule to maximize compliance and share resources. Finally, a new vaccine should not prime individuals for atypical measles on exposure of immunized individuals to wild-type MV (a complication of formalin-inactivated measles vaccines) and should not be associated with prolonged immunosuppression adversely affecting immune responses to subsequent infections (a complication of high-titre measles vaccines). Several vaccine candidates with some of these characteristics are undergoing development and testing. Naked cDNA vaccines are thermostable, inexpensive and could theoretically elicit antibody responses in the presence of passively acquired maternal antibody. DNA vaccines encoding either or both of the measles H and F proteins are safe, immunogenic and protective against measles challenge in naive, juvenile rhesus macaque monkeys 65 . A different DNA vaccine, containing H, F and N genes and an IL-2 molecular adjuvant, provided protection to infant macaques in the presence of neutralizing antibody 66, 67 . Alternative vectors for administering MV genes, such as alphavirus 68 , parainfluenza virus 69 or enteric bacteria 70 are also under investigation. Immune responses to intranasal administration of MV vaccines are enhanced by the use of adjuvants 71 . Novel oral immunization strategies have been developed using plant-based expression of the H protein from MV in tobacco 72 . The elimination of measles in large areas, such as the Americas, suggests that global measles elimination is feasible with current vaccination strategies 25, 73, 74 . The American Red Cross, along with other partners, has played a significant role in establishing the Measles Initiative, which has been responsible for much of the success in measles control in Africa and is committed to reducing global measles deaths by providing measles vaccine to children. The key questions regarding global measles elimination are whether epidemiological conditions are sufficiently different in parts of Africa and Asia to hinder measles-elimination efforts, and whether there will be the political and public support necessary for elimination activities. Interruption of MV transmission could be difficult in densely populated urban environments of Africa and Asia and in regions of high HIV-1 prevalence 24 . Other potential obstacles to global measles elimination have been identified, including immunity that decreases over time, and the possibility of transmission from subclinical cases, although these have not hindered measles elimination in the Americas 24,75 . Measles outbreaks have occurred in susceptible adults, most notably the large outbreak in Sâo Paulo, Brazil, in 1997, but have not led to sustained endemic transmission 24 . Garnering the political will and public support is likely to be more difficult in resource-rich countries where the burden of disease due to measles is not recognized and unfounded fears of serious adverse events from vaccination are more prevalent. Whether the threat from bioterrorism precludes stopping measles vaccination after eradication is a topic of debate, but, at the least, a single-dose rather than a two-dose measles vaccination strategy could be adopted 73 . The measles eradication end game is likely to be different from that for smallpox and polioviruses 76, 77 . Higher levels of population immunity are necessary to interrupt MV transmission. Linking surveillance activities with rapid outbreak response and vaccination efforts will be crucial for measles elimination, as it is for smallpox and polio. However, the rapid spread of MV and the need for trained healthcare workers to administer measles vaccine will be additional obstacles. Ensuring that the necessary supply of measles vaccine is maintained as elimination efforts progress will also be crucial, requiring close collaboration with vaccine manufacturers. Critics of eradication programmes claim that they divert resources from primary healthcare and are imposed on countries or communities from outside. Enormous resources and efforts might be required to eradicate the few remaining cases of disease, and the economic and social costs of eradication need to be considered 78 . The polio eradication campaign in Nigeria was seen to trigger a massive outbreak response for three confirmed cases in Adamawa State in 2005, whereas hundreds of deaths due to measles did not result in a comparable response 79 . But delays in achieving polio eradication will make many people hesitant to move forward with measles eradication. 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The authors declare no competing financial interests.