key: cord-1045954-nh4fbls5
authors: Hidalgo, Andrés
title: A NET-thrombosis axis in COVID-19
date: 2020-09-03
journal: Blood
DOI: 10.1182/blood.2020007951
sha: 7321aa342153eafce0e13cc635f3e3fa64fa8fcd
doc_id: 1045954
cord_uid: nh4fbls5

Patients with COVID-19 are susceptible to thrombosis and multiorgan failure. In a prospective study in this issue of Blood, Middleton et al(1) identify neutrophil extracellular traps (NETs) as the potential culprits of COVID-19-related pulmonary dysfunction and death.

mature RBC immunophenotype. Thus, the authors speculated that the main value of stress erythropoiesis may be to generate a wave of erythrocytes that are resistant to infection-or inflammation-induced hemolysis and are resilient enough to maintain homeostasis until BM steady-state erythropoiesis restarts. 5 This is certainly an intriguing model, but such a model requires additional investigation.

These findings are notable for their implications for prior investigations of HSC heterogenity. Previous reports described heterogeneous expression of CD86 among HSCs (CD48 -CD150 1 LSK and CD34 -Flk2 -LSK), with CD86 -HSCs expanding during aging or with chronic LPS treatment. These CD86 -HSCs showed poor longterm reconstitution activity in transplant experiments as well as myeloid-biased differentiation compared with CD86 1 HSCs. 6 The Kanayama et al study suggests that the CD86 -HSCs identified after LPS challenge may simply represent MPs contaminating the immunophenotypically defined HSC pool. Thus, it is possible that CD86 high expression among LSK cells can help identify aged HSCs with the highest levels of self-renewal and balanced lineage output.

The ability to more accurately identify HSPCs during stress will likely promote exploration of several unresolved questions in hematopoiesis. Although the authors have tested CD86 in the context of experimental models of acute inflammation, it is important to determine whether CD86 can help resolve HSPC populations under other conditions associated with inflammation. For example, conditioning regimens such as irradiation create an inflammatory microenvironment into which newly transplanted HSPCs enter, but using CD86 may allow investigators to more accurately determine the fate of MP and HSC populations immediately after BM transplantation. Given that IFN-g has been reported to cause impaired human HSC self-renewal and induce myeloid lineage bias, 7 these studies also raise the question of whether isolation of human HSCs and committed myeloid progenitors under inflammatory conditions is compromised by changes in the expression of cell surface markers such as CD38, CD90, CD123, and CD45RA. 8 Studies of human hematopoiesis under inflammatory stress are few, and thus we anticipate such studies to be the subject of future investigations. With .14 million affected individuals and a half million deaths now officially caused by SARS-CoV-2 infection worldwide, this coronavirus has confirmed the predictions of global viral spread into a pandemic with major public health and social implications. Beyond these critical considerations, COVID-19 has also caused clinicians and basic scientists to reexamine our models of immune and inflammatory responses against pathogens. One of these models deals with immune mechanisms that trigger coagulopathy, a common clinical manifestation seen in COVID-19 patients (see figure) . 2 Coagulopathy is a hypercoagulable state that can inflict irreversible damage to the lungs and other organs. At the same time, an additional hallmark seen in severe cases of COVID-19 is the presence of elevated neutrophil counts, 3 a feature that is shared with other forms of cardiovascular disease.

How are coagulopathy and increased neutrophils related in the context of COVID-19? By searching for potential pathogenic mechanisms, Middleton et al report in this

Detail of neutrophils (gray) bearing markers of NETs (citrullinated histone; green), trapped in a platelet-rich clot (red). These microthrombi are found in the lungs of patients with severe COVID-19. Scale bar, 20 mm. See Figure 3A in the article by Middleton et al that begins on page 1169. blood® 3 SEPTEMBER 2020 | VOLUME 136, NUMBER 10 issue of Blood that neutrophils from COVID-19 patients are more prone to release NETs than neutrophils from healthy individuals, or from patients displaying milder forms of the disease. NETs are multimolecular, DNAbased complexes released by neutrophils that allow containment and killing of bacteria and fungi and can also trap and deactivate virus. 4 Unfortunately, NETs are also thrombogenic because they contain highly cationic proteins. 5 Thus, the particular NETforming propensity of neutrophils in some COVID-19 patients may be causally associated with the development of acute and severe coagulopathies, even though no such patients were identified in the cohort from this study. More generally, the current findings suggest that the SARS-CoV-2 virus elicits potent immunothrombosis, a protective process that allows the containment of pathogens. Both the presence of thrombi and nuclear and granular proteins contained within the DNA lattice that form NETs create a cytotoxic milieu when neutrophils are recruited en mass, thereby compromising epithelial and vascular integrity and contributing to rapid pulmonary dysfunction. Consistent with this notion, Middleton et al find that the presence in blood of NET byproducts, such as DNA free or associated with myeloperoxidase, correlated well with parameters of lung damage in the patients.

Contrasting with these findings, however, the authors found no correlation between NETs and markers of endothelial damage or active thrombosis, such as D-dimers or von Willebrand factor. This suggests that the NET-thrombosis connection may be more complex than anticipated, possibly because activated platelets and factors of the coagulation cascade interact in undefined ways (by interfering or promoting) with the formation of NETs. Alternatively, the NET-thrombosis connection may be obscured because quantification of NETs in plasma is imprecise, for example, because NETs deposited in affected organs may be no longer detectable or because they are being actively degraded. This limitation raises caution regarding the use of absolute NET levels as a prognostic score for COVID-19 patients.

Among the several features reported here, the authors note that neutrophils from severe COVID-19 cases are more granular. Beyond denoting possible activation of these leukocytes, this finding is more likely to associate with the presence of immature granulocytic cells in the circulation of severe COVID-19 patients. This may be important because mobilization of immature neutrophils from the bone marrow, the organ in which granules are synthesized, implies higher content of cytotoxic compounds or NET-inducing enzymes in these cells and further provides links with studies demonstrating a positive correlation between disease severity and the presence of "developing" neutrophils in the circulation of COVID-19 patients. 6 Consistently, studies in mice have demonstrated that higher granule content correlates with the ability of neutrophils to form NETs in models of acute pulmonary inflammation, and that hypergranular neutrophils are those recently released from the marrow. 7 These findings, however, contrast with the observation in this and a previous report that plasma from severe COVID-19 patients contains factors that render neutrophils prone to form NETs, suggesting that both cell-intrinsic and environmental changes can incite neutrophil-mediated injury (this study and Zuo et al 8 ) .

Finally, the present study shows that NET formation by neutrophils from severe COVID-19 patients can be effectively blocked by an endogenous inhibitory peptide ex vivo, raising the possibility of using NET-inhibitory or degrading compounds to protect patients from the most severe forms of COVID-19. Although this approach is indeed appealing, and these authors and others have previously discussed various approaches to target NETs to blunt lung injury in COVID-19 patients, 9 it remains critically important to provide causal data to support the involvement of NETs in the pathology of COVID-19. This has been hampered so far by the challenge of developing appropriate animal models (eg, animals expressing human ACE2 at the correct locations), and by the strict biosafety conditions needed for manipulation of the virus. Furthermore, larger cohorts of patients will be needed for the prospective or retrospective determination of NET levels in plasma and other tissues, the presence of distinct neutrophil subsets, or NET-inducing factors in order to substantiate the conclusions made here.

Nonetheless, this study represents a much needed effort if we are to identify new strategies to efficiently protect patients from the devastating consequences of SARS-CoV-2 infection.

CD86-based analysis enables observation of bona fide hematopoietic responses

Conversion of danger signals into cytokine signals by hematopoietic stem and progenitor cells for regulation of stress-induced hematopoiesis

Re-entry into quiescence protects hematopoietic stem cells from the killing effect of chronic exposure to type I interferons

Neutrophil extracellular traps contribute to immunothrombosis in COVID-19 acute respiratory distress syndrome

COVID-19 coagulopathy vs disseminated intravascular coagulation

Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China

Virus-induced NETs-critical component of host defense or pathogenic mediator?

Extracellular DNA traps promote thrombosis

A single-cell atlas of the peripheral immune response in patients with severe COVID-19

Programmed "disarming" of the neutrophil proteome reduces the magnitude of inflammation

Neutrophil extracellular traps in COVID-19

Conflict-of-interest disclosure: The author declares no competing financial interests. n