key: cord-1044277-a7cctnmp
authors: Lu, Xuan; Tang, Liang V.; Wang, Hua-Fang; You, Yong; Wang, Ya-Dan; Hu, Yu; Shi, Wei; Xia, Ling-Hui
title: The great challenge of managing recipients of hematopoietic stem cell transplantation combined with COVID-19
date: 2020-08-25
journal: Bone Marrow Transplant
DOI: 10.1038/s41409-020-01035-6
sha: e3f5976cc82f8686bae658629de184c33885fb6d
doc_id: 1044277
cord_uid: a7cctnmp

nan

Coronavirus disease 2019 (COVID-19) pandemic is putting enormous pressure on the health care throughout the world. To date, there is still the absence of a specific antiviral agent and vaccine against the lethal disease [1] . Currently, more than 40,000 cases receive hematopoietic stem cell transplantation (HSCT) in the world each year, and the ultimate impact of COVID-19 on the field of HSCT is still unknown [2] . However, in consideration of the co-existence of the severe immune deficiency and other medical comorbidities, HSCT recipients, who are frequently exposed to severe immunodeficiency may have different COVID-19 features, are at the highest risk for critical COVID-19 illness, and will bear the worst clinical outcome [3] . Specialists have issued interim recommendations for recipients and donors of HSCT [4, 5] . Herein we present two HSCT recipient cases affected by COVID-19 from Wuhan, the first epicenter of the pandemic, aiming to share our experience and lessons.

Patient 1 is a 20-year-old man who was diagnosed with acute lymphoblastic leukemia in July 2017. He received allogeneic HSCT in December 2018. The myeloablative conditioning regimen is busulfan, cyclophosphamide, and etoposide. Stem cell donor was his mother, who is HLA 7/10 matched with him. Antithymocyte globulin (ATG), tacrolimus, mycophenolate mofetil (MMF), methotrexate, and basiliximab were employed for prevention of graft-versus-host disease (GVHD). Following transplantation, a complete donor chimerism was achieved, and no minimal residual disease or abnormal gene mutations were identified in the patient's bone marrow. The maintenance anti-GVHD therapy included tacrolimus, MMF, and ruxolitinib.

In January 2020, he received a donor lymphocyte infusion because of relapse. Upon myelosuppression, the patient developed fever and cough. CT scan showed bilateral patchy ground-glass opacities, more lesions in the lower lobe of the right lung compared with the last imaging. Because such imaging abnormalities as "groundglass opacities" and lymphocytopenia had already been observed in the regular check-ups following transplantation (Table 1) , no special therapy was given. His body temperature increased rapidly, up to to 40°C. Thereafter SARS-CoV-2 RNA tests for pharyngeal swab were performed, which turned out to be repeated positive, and the patient was diagnosed as COVID-19. The initial laboratory findings were as follows: lymphocyte 0.93 G/L, platelet 102 G/L, procalcitonin 0.32 ng/ml, C-reactive protein 13.1 μg/L, and D-dimer 1.65 μg/mL. Immunosuppressive agents and JAK1/2 inhibitor were withdrawn. Antiviral drug (redesivir 200 mg, once daily), oxygen inhalation, and supportive treatment were prescribed. Antibiotics were prescribed throughout the clinical course, including meropenem, linezolid, and teicoplanin.

Eight days following onset, the patient presented with persistent hyperthermia, red papules, abdominal pain, and diarrhea with hematochezia of 400-1500 ml/day. In consideration of virus infection-induced GVHD, methylprednisolone 40 mg/day and ruxolitinib of 20 mg/day were used. However, pneumonitis became rapid deterioration as shown in the bedside chest X-ray, in which bilateral large blurring shadows were found. Procalcitonin level increased rapidly from 2.9 to 59.1 ng/ml within 48 h, and the patient died of severe sepsis and cardiopulmonary arrest (Fig. 1a) . The myeloablative conditioning regimen was fudarabine, busulfan, and cytarabine. Stem cell donor was her father, who is HLA 7/10 matched with her. ATG, tacrolimus, MMF, methotrexate, and basiliximab were employed for prevention of GVHD. She received further posttransplant therapy such as interleukin 2 and donor lymphocyte infusion. Then a complete donor chimerism was achieved, and no minimal residual disease was identified. The maintenance anti-GVHD therapy included tacrolimus, MMF, and ruxolitinib.

In January 2020, however, she was admitted to hospital due to extramedullary relapse of leukemia in the uterus and pancreas. During hospitalization, she began to have fever on February 6. The highest body temperature was 38.7°C, accompanied by cough and sputum. Pulmonary CT imaging showed bilateral patchy ground-glass opacities. Because a number of patients in the same ward had been infected with SARS-CoV-2, COVID-19 was suspected. RNA test was positive 2 days later. The initial laboratory findings were as follows: lymphocyte 0.98 G/L, platelet 48 G/L, procalcitonin 0.37 ng/ml, C-reactive protein 98.2 μg/L, and D-dimer 8.16 μg/mL. Antiviral agent (umifenovir 200 mg, three times a day), antibiotics, and low-dose corticosteroid (methylprednisolone 20 mg/day) were prescribed, while immunosuppressive agents and JAK1/2 inhibitor were withdrawn. During the whole clinical course, the lowest SPO 2 was 93% and gradually returned to 98% after oxygen inhalation by face mask, therefore the ventilator was not employed. Antibiotics injection included meropenem, linezolid, and caspofungin. 16 days following onset, tests for virus RNA turned out to be negative. Although additional CT scan also suggested a partial resolution, the patient suffered from major depression and rejected further treatment (Fig. 1b) .

Several case reports have focused on solid organ transplant recipients [6] [7] [8] . A cohort study suggested that kidney transplant recipients had a poor prognosis once suffering from COVID-19, with a case fatality rate up to 28% at 3 weeks [9] . This rate seems similar to that in cancer patients with COVID-19 (~25%) [10] , higher than that reported among patients with COVID-19 who are older than 70 years (8-15%), much higher compared with that in general COVID-19 population (1-5%). There are new challenges for HSCT physicians during the new pandemic (Fig. 2) . Compared with solid organ transplantation, immunosuppressive status after receiving HSCT is much more serious, due to severe myelosuppression and using various immunosuppressive agents including ATG [11] . Therefore, infection is likely to deteriorate much more rapidly. GVHD is a unique complication of HSCT, which is usually induced and aggravated by viral infection. GVHD cause pulmonary impairment, promoting ARDS and respiratory failure. Anti-GVHD required immunosuppressants and JAK1/2 inhibitor. However, if these agents were prescribed, viral infection will deteriorate and if not, GVHD will progress.

It is worth noting that both cases did not develop antibodies to SARS-CoV-2 (IgM and IgG). It is probable that immune system was unable to produce antibodies effectively, attributed to severe malnutrition and lymphocyte dysfunction in HSCT recipients. These patients are likely at high risk for reinfection.

Lymphocytopenia is frequently reported with COVID-19, occurring in~65-80% of patients. And "ground-glass opacities" in CT imaging is another typical feature. Nevertheless, because of the long-term immunocompromised state, chronic infection, "ground-glass opacities" in the lungs, and decrease of white blood cell and lymphocyte in circulation are common in patients after receiving HSCT, as is shown in both cases (Fig. 3) . Therefore, it is relatively hard to make a timely differential diagnosis from COVID-19. Severe COVID-19 will cause thrombophilia, presenting as elevated D-dimer levels [12] . We were in a dilemma whether to use anticoagulant agents or not for both cases, considering thrombocytopenia and hematochezia, which are often observed in HSCT recipients with primary disease relapse or with GVHD. Moreover, suffering from triple whammy (hematological malignancy, HSCT, and COVID-19), patients are prone to depression.

Collectively, HSCT recipients have a poor clinical outcome under the COVID-19 crisis. Exploring the comprehensive management of HSCT recipients with COVID-19 is urgently needed. Author contributions LVT, and XL, obtained and analyzed the clinical data. LVT, and WS, made the figures. We all contributed to caring for the patient, editing the figures, and writing and editing the manuscript.

Conflict of interest The authors declare that they have no conflict of interest. Ethics approval and consent to participate The parents of case 1 case 2 have approved to use the data.

Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

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