key: cord-1039928-ed6xu12w
authors: Briggs, Jessica
title: Validation of Pooled SARS-CoV-2 Testing in the Outbreak Setting
date: 2021-03-08
journal: Am J Trop Med Hyg
DOI: 10.4269/ajtmh.21-0202
sha: 762ae55ad7ad22f6e7993b59130e715e5d409222
doc_id: 1039928
cord_uid: ed6xu12w

nan

However, detailed published data on the pragmatic use of pooled testing in outbreaks in resource-limited settings are lacking. Therefore, the study published in this issue of AJTMH by Thanh et al. 13 is particularly timely. After 100 days without community transmission in Vietnam, two individuals with COVID-19 disease were admitted to two different hospitals in Da Nang, triggering a massive outbreak investigation. Contact tracing and testing detected cases directly linked with index cases at Da Nang Hospital. In parallel, the city was locked down on July 28, 2020, and mass community testing using a pooling strategy was performed to target those at greatest risk of infection. The study team's unique approach involved pooling all nasopharyngeal swab samples from each household (2-7 swabs per household) into viral transport medium. If a pool was positive, each household member was retested within 48 hours; 96,123 asymptomatic people met the criteria for testing, resulting in 22,290 pooled samples, of which only 24 were positive, resulting in the retesting of 104 individuals. This approach led to the identification of 32 confirmed cases, of which 22 had no history of contact with a confirmed case and would have been missed by standard contact tracing. By September 11, 2020, the city had gone 12 consecutive days without community transmission, representing a massive success in controlling the nascent outbreak. Importantly, the testing strategy required only 14 days compared with a predicted 64 days with individual testing, and allowed for a 77% cost reduction compared with testing every sample individually. In addition, pooling swabs into media (instead of pooling media from individual swabs or after RNA extraction) saved a substantial amount of freezer space, an often overlooked consideration in low-resource settings. In addition, there was no difference in the CT values of positive pooled versus positive individual samples.

Two other groups have published their experience with implementation of pooled testing for SARS-CoV-2 at a large scale. In Israel, pooled testing of samples before RNA extraction was highly efficacious despite community prevalence fluctuating between 0.5% and 6%, and cost savings of 76% were realized. 14 Notably, their method involved dynamically decreasing the pool size as prevalence rose in the community, which increased logistical challenges but improved efficiency. In India, a pooled testing strategy was piloted during an outbreak; pools of five samples were created before RNA extraction. 15 Although a cost reduction of 68% was noted, single positives (all with high CT values, i.e., low viral loads) in four of 109 pools were missed.

Overall, there is increasing evidence for the efficacy of pooled testing strategies for the detection of SARS-CoV-2 infection in asymptomatic individuals. The study by Thanh et al. 13 published in this issue clearly shows the utility of pooled testing in a low-resource setting to screen a large asymptomatic population and prevent the expansion of a COVID-19 outbreak. Notably, this success was in conjunction with other aspects of a strong public health response, including a city-wide lockdown and rigorous contact tracing. This is a quite different situation than in many higher resource countries, where transmission remains high and pooling samples may not be cost-effective. However, pooled testing is likely to become more attractive in other settings as vaccination and other factors drive down community transmission. There is now sufficient evidence that pooled testing for SARS-CoV-2 is sensitive enough to detect individual positive samples and mounting evidence that using this testing strategy in outbreak settings can be highly effective in detecting asymptomatic infections and reducing costs. We should look to the practical experience and success of those in lowresource settings as we consider expanding pooled testing for SARS-CoV-2 infection in the United States and other highresource settings.

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