key: cord-1038044-bf4v6g39 authors: Hamam, O.; Goda, A.; Eldalal, M.; Ussama, A.; Elmandouh, O.; Fahmy, M.; Elyamany, K.; Ikram, W.; Mahdy, A.; Bhandari, R.; Asal, H.; Egbe, A. title: Cardiac Arrhythmias in Patients with COVID-19: A Systematic review and Meta-analysis date: 2020-10-13 journal: nan DOI: 10.1101/2020.10.09.20209379 sha: c50642856aab65f759f987e5ea1540b234995b54 doc_id: 1038044 cord_uid: bf4v6g39 Background: Cardiac arrhythmia cannot be overlooked in patients with coronavirus disease 2019 (COVID-19) as it carries a great influence on the outcomes. Hence, this study aimed to build concrete evidence regarding the incidence of cardiac arrhythmia in patients with COVID-19. Methods: We performed a systematic search for trusted databases/search engines including PubMed, Scopus, Cochrane library and web of science. After screening, the relevant data were extracted and the incidences from the different included studies were pooled for meta-analysis. Results: Nine studies were finally included in our study consisting of 1445 patients. The results of meta-analysis showed that the incidence of arrhythmia in patients with COVID-19 was 19.7% with 95% confidence interval (CI) ranging from 11.7 to 27.6%. There was also a significant heterogeneity (I2 = 94.67%). Conclusion: Cardiac arrhythmias were highly frequent in patients with COVID-19 and observed in 19.7% of them. Appropriate monitoring by electrocardiogram with accurate and early identification of arrhythmias is important for better management and outcomes. • Cardiac arrhythmia cannot be overlooked in patients with coronavirus disease 2019 (COVID-19) as it carries a great influence on the outcomes. • This study aimed to build concrete evidence regarding the incidence of cardiac arrhythmia in patients with COVID-19. • Cardiac arrhythmias were highly frequent in patients with COVID-19 and observed in 19.7% of them. • Appropriate monitoring by electrocardiogram with accurate and early identification of arrhythmias is important for better management and outcomes. . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 13, 2020. . https://doi.org/10.1101/2020.10.09.20209379 doi: medRxiv preprint . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 13, 2020. . https://doi.org/10.1101/2020.10.09.20209379 doi: medRxiv preprint Coronavirus disease 2019 (COVID-19) has become a global health concern affecting the lives of millions around the world 1 . It is caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The clinical manifestations can differ from very mild to highly severe with the mean affection of the respiratory system which can lead to pneumonia as well as respiratory distress syndrome 2,3 . Moreover, the associated involvement of other systems such as the cardiovascular system cannot be overlooked as it carries a great influence on the outcomes 2-6 . In a recent published report, cardiac involvement was showed to occur in COVID-19 even without manifestations of respiratory tract involvement 7 . Several studies asserted upon the incidence of cardiac injury in COVID-19 5,8 . Among those reported cardiac manifestations, cardiac arrhythmias were observed with variable estimates among studies and according to severity levels 2,6 . Cardiac arrhythmias appear to be one of the highly challenging complications of COVID-19 and several speculations about different mechanisms were reported with a key role for proinflammatory mediators. Some of ion channels can be affected due to COVID-19 which may lead to subsequent alterations in cardiac conduction or repolarization. Thus, it can increase the risk for cardiac arrhythmogenesis to occur 9-12 . Wang et al 2 was the first to report an incidence of 17% of arrhythmias in COVID-19 patients. Most of those cases with arrhythmias were transferred to intensive care unit. Likewise, more and higher incidence of arrhythmias (44%) was reported in studies involving patients in the intensive care unit 2,6 . Therefore, a better understanding of the magnitude and role of cardiac arrhythmias in COVID-19 is essential for . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) The copyright holder for this preprint this version posted October 13, 2020. . https://doi.org/10.1101/2020.10.09.20209379 doi: medRxiv preprint better monitoring and management. Hence, this systematic review and meta-analysis aimed to build concrete evidence regarding the incidence of cardiac arrhythmia in COVID-19. We adhered to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and Cochrane's handbook of systematic reviews to conduct this study 13, 14 . We combined the following keywords and conducted our search: "COVID-19", "SARS-CoV-2", "cardiovascular" and "Arrhythmia". We searched PubMed, Web of Science, Scopus, and Cochrane Library for relevant articles to be included. An additional online and manual search was performed on Google Scholar and Preprint Servers to ensure adequate inclusion of all studies. Results were imported into Endnote X8 (Thompson Reuter, CA, USA) for duplicates deletion. We included valid case series (>10 patients) and cohort studies including adults with COVID-19 with incidence of arrhythmia. Review articles, editorial, commentaries were excluded. The first author (O.H) divided other authors into two teams; each team independently performed title and abstract screening. Then, each team obtained the full-text of the included papers and performed full-text screening. Any disagreement between the two teams was resolved through consultation with the study senior (A.E). . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 13, 2020. . https://doi.org/10.1101/2020.10.09.20209379 doi: medRxiv preprint 6 The two teams extracted the data; one team performed extraction of selected outcomes and the other team extracted baseline data, then, data were revised in a cross-revision manner. Extracted data include author, country, year, study design, age, sex, total number of patients, and number of patients with arrhythmia. We used the Newcastle-Ottawa scale (NOS) which is available at (https://www.ohri.ca/programs/clinical_epidemiology/oxford.asp) for assessing the risk of bias for our included studies. The possible scores of this scale range from 0 to 9. Studies with a score of seven to nine, four to six, and zero to three were classified as studies with low, moderate, and high risk of bias, respectively. The meta-analysis of the included studies was performed using OpenMeta [Analyst] version 1.15 for conducting single-arm meta-analysis. Meta-analysis for proportions was utilized to pool the incidence of arrhythmia in the groups. Dichotomous data were calculated to obtain risk ratios along with their 95% confidence intervals (CIs). Heterogeneity among studies was assessed using the I 2 test and P-value from the chi-squared test of heterogeneity. Values of I 2 >50 and P<0.1 are significant markers of heterogeneity among studies according to Cochrane's handbook 14 . Random effect models were used to avoid the effect heterogeneity. The statistical significance was set with P-value at 0.05. According to Egger and colleagues, assessment of publication bias using funnel plot method and Egger's test is unreliable for less than 10 included . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 13, 2020. . https://doi.org/10.1101/2020.10.09.20209379 doi: medRxiv preprint studies. Therefore, in the present study, we could not assess for publication bias due to small number of included studies 15, 16 . We searched the aforementioned search engines/databases and found 955 studies after duplicate removal. We excluded 946 studies as they were not eligible for inclusion according to eligibility criteria, and a total of nine studies were finally included in our study consisting of 1445 patients 2,5,6, [17] [18] [19] [20] [21] [22] . Figure (1) shows a summary of our search and table (1) shows the summary of the included studies. Baseline characteristics are displayed in table 1. Eight of the included studies were conducted in China while only one study reported from USA. Among the included studies, the highest mean age was 71 years while the lowest was 55 years. Most of the included studies have a male predominance reaching 75% of the total included patients. Among our nine studies evaluated for the risk of bias, all of our studies had a low risk of bias with score of seven or higher (Table 1) . . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 13, 2020. . https://doi.org/10.1101/2020.10.09.20209379 doi: medRxiv preprint 8 The results of meta-analysis including the nine studies showed that the incidence of arrhythmia in patients with COVID-19 was 19.7% with the 95% confidence interval (CI) of 11.7 to 27.6%. There was significant heterogeneity (I 2 = 94.67%) which can be attributed to the variability of reported incidences among the different included studies (Figure 2 ). Previous studies has showed that compared to different systems, the cardiovascular system is considered the second most commonly affected organ by COVID-19 after the respiratory system 2,5-8, 23 . The results of this systematic review and meta-analysis showed that cardiac arrhythmia occurred frequently in patients with COVID-19 with incidence exceeding 19% of the total cases with covid-19. This is consistent with other individual studies reported firstly from china such as Wang et al 2 who reported that cardiac arrhythmia occurred in 23 patients out of 138 patients included in their study. In our study, the cumulative number of patients with both arrhythmia and COVID-19 was 269 out of 1445 patients with COVID-19 which is slightly of higher percentage than Wang et al 2 . It is known from previous meta-analyses that baseline characteristics such as sex, hypertension, and diabetes can affect the outcomes of the pooled studies 23-27 but the metaregression option was not available to assess the exact effect of those factors given that the number of the included studies was less than 10. There are different documented speculations to explain the association of arrhythmia with COVID-19. For instance, this may be correlated with metabolic disruption, hypoxia, proinflammatory process, or different stressors associated coming on the line with COVID-19 28 . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 13, 2020. . https://doi.org/10.1101/2020.10.09.20209379 doi: medRxiv preprint Moreover, some of ion channels can be affected due to COVID-19 which may lead to subsequent alterations in cardiac conduction or repolarization. Thus, it can increase the risk for cardiac arrhythmia to occur 9-11 . We noticed significant heterogeneity while performing this meta-analysis. This can be explained by the different and variable estimates among the included studies which were the highest in Du et al al. 20 reaching 60%. It is also worth noting that our included studies did not mention the types of arrhythmia in specific which prevent us from subgrouping the incidence for each type. This study can be considered the most updated and comprehensive study to assess the incidence of COVID-19 in a suitable number of patients. However, this study suffered from several limitations. Although the number of patients from our pooled studies was 1445 patients, the number of studies was only nine which lead us to avoid testing for publication bias and performing meta-regression according to the reported recommendations for those tests that need at least 10 studies to be well performed. Moreover, the types of cardiac arrhythmias were not specified so we could not provide more specific subgroup analysis based upon the included studies. The studies were all observational retrospective and this type of studies has its own known limitations. Further studies that can explore cardiac arrhythmias in COVID-19 patients with more details on determinants are highly recommended. To recapitulate, cardiac arrhythmias were highly frequent in patients with COVID-19 and observed in 19.7% of them. Appropriate monitoring by electrocardiogram with accurate and early identification of arrhythmias is important for better management and outcomes. . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 13, 2020. Authors declare no Conflict of Interests for this article. . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) The copyright holder for this preprint this version posted October 13, 2020. is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. 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The copyright holder for this preprint this version posted October 13, 2020. . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted October 13, 2020. . CC-BY 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review)The copyright holder for this preprint this version posted October 13, 2020. . https://doi.org/10.1101/2020.10.09.20209379 doi: medRxiv preprint