key: cord-1036409-rbye73yv
authors: Xu, Jiuyang; Jia, Wenxu; Wang, Pengfei; Zhang, Senyan; Shi, Xuanling; Wang, Xinquan; Zhang, Linqi
title: Antibodies and vaccines against Middle East respiratory syndrome coronavirus
date: 2019-06-06
journal: Emerg Microbes Infect
DOI: 10.1080/22221751.2019.1624482
sha: 1adf578fb1c7019c2293b2e9c7cc3f036f9bc15b
doc_id: 1036409
cord_uid: rbye73yv

The Middle East respiratory syndrome coronavirus (MERS-CoV) has spread through 27 countries and infected more than 2,200 people since its first outbreak in Saudi Arabia in 2012. The high fatality rate (35.4%) of this novel coronavirus and its persistent wide spread infectiousness in animal reservoirs have generated tremendous global public health concern. However, no licensed therapeutic agents or vaccines against MERS-CoV are currently available and only a limited few have entered clinical trials. Among all the potential targets of MERS-CoV, the spike glycoprotein (S) has been the most well-studied due to its critical role in mediating viral entry and in inducing a protective antibody response in infected individuals. The most notable studies include the recent discoveries of monoclonal antibodies and development of candidate vaccines against the S glycoprotein. Structural characterization of MERS-CoV S protein bound with these monoclonal antibodies has provided insights into the mechanisms of humoral immune responses against MERS-CoV infection. The current review aims to highlight these developments and discuss possible hurdles and strategies to translate these discoveries into ultimate medical interventions against MERS-CoV infection.

The rapid emergence and dissemination of infectious diseases has taken a heavy toll on humans since the beginning of the twenty-first century. One of the most well-known examples was the outbreak of severe acute respiratory syndrome (SARS) in the winter of 2002 and 2003, caused by a novel coronavirus (SARS-CoV) [1, 2] . In distinct contrast to the mild human coronaviruses HCoV-229E [3] , HCoV-OC43 [4] , HCoV-NL63 [5] , and HCoV-HKU1 [6] , infection with SARS-CoV frequently resulted in severe symptoms including fever, dry cough, shortness of breath and pneumonia. Transmission of SARS-CoV was primarily from person to person and most cases occurred in health care settings lacking adequate infection control precautions [2] . The SARS outbreak had severe consequences in 29 countries and regions, infecting 8096 people worldwide with a fatality rate of approximately 10% [7] . There are still no vaccines or therapeutics specific to SARS-CoV available 16 years after the SARS outbreak. It is not hard to imagine how catastrophic it would be if SARS-CoV were to hit the human community again.

While SARS-CoV remains a mystery and a loose cannon, another novel coronavirus emerged in Saudi Arabia in 2012, later known as the Middle East respiratory syndrome coronavirus (MERS-CoV) [8] . The fatality rate of MERS-CoV infection is approximately 35 .4%, and new cases as well as associated deaths continue to arise to date [9] . Despite that most cases have been attributed to human-to-human transmission, MERS-CoV does not appear to transmit efficiently among humans unless there is close contact. The exact source of MERS-CoV and its routes of transmission to humans still remain uncertain. Dromedary camels are believed to be the animal reservoir for MERS-CoV because isolates from camels are almost identical to those from human, and that many domestic camels are seropositive for MERS-CoV (reviewed in [10, 11] ). Furthermore, current evidence strongly suggests that bats are the original source for MERS-CoV, as many coronaviruses phylogenetically related to MERS-CoV originate in bats, including BatCoV-HKU4, BatCoV-HKU5 and other MERS-related coronaviruses [12] [13] [14] [15] . The BatCoV-HKU4 was also shown to be able to engage the cellular receptor of MERS-CoV, adding evidence to the bat origin theory [16] . However, there has not yet been direct evidence for isolating MERS-CoV from bats (reviewed in [10, 11, 17] ).

Great efforts have been made to develop preventive and therapeutic interventions against MERS-CoV infection. In particular, monoclonal antibodies and vaccines targeting the Spike glycoprotein are major areas of focus due to its critical role in mediating viral entry, and its potential in inducing protective antibody responses in infected individuals. So far, more than twenty monoclonal antibodies with nanomolar neutralizing activities have been reported and many vaccine candidates are underway in preclinical and clinical studies. In this review, we aim to capture the current advances and discuss possible strategies to translate these discoveries into an ultimate medical intervention against MERS-CoV infection.

MERS-CoV belongs to the genus betacoronavirus of the coronaviridae family [18] . It is an enveloped, single-stranded, positive-sense RNA virus with a helical capsid structure (Figure 1(A) ). The genome of MERS-CoV is around 30 kb (30,119nt) long and encodes 4 structural proteins (Spike, Envelope, Membrane, and Nucleocapsid) and 16 nonstructural proteins (Figure 1 (C)) [13] . Like other coronaviruses, the MERS-CoV uses its spike (S) glycoprotein to interact with cellular receptors and enter into the target cell [19] [20] [21] [22] . As a unique structural component of the virion membrane, the S glycoprotein assembles into trimers and forms large protruding spikes on the surface of the virion [20] . The S glycoprotein is a typical type I membrane glycoprotein consisting of a globular S1 domain at the N-terminal, followed by a membrane-proximal S2 domain and a transmembrane (TM) domain [21] . The S1 domain mediates viral attachment and contains the RBD (receptor binding domain), which determines the host range and cellular tropism for MERS-CoV [23] [24] [25] . Similar to other coronaviruses, the S2 domain of MERS-CoV, mediating membrane fusion, contains the hydrophobic fusion peptide (FP) at the N-terminus as well as two heptad repeats designated as HR1 and HR2 (Figure 1 (C)) [26] . Through co-purification with the MERS-CoV S1 domain, Raj and colleagues identified that dipeptidyl peptidase 4 (DPP4, also known as CD26) functions as a cellular receptor for MERS-CoV [27] .

The MERS-CoV virion enters the host airway cells in the respiratory tract through fusion with either the plasma or endosomal membrane [19] . Binding between RBD and the cell receptor triggers a cascade of conformational changes that lead to the formation of a pre-hairpin intermediate of S2, in which the hydrophobic HR1 is exposed and allows the fusion peptide to insert into the target cell membrane. This transient S2 intermediate then refolds with HR2 into a stabilized trimer of hairpins, also called six-helix bundle structure (6-HB), which brings the target cell membrane into close proximity of the viral envelope, resulting in the completion of the fusion process and initiation of the virus life cycle [21] (Figure 1(B) ). Structure-based design of various peptides able to block the formation of 6-HB have demonstrated potent inhibition on MERS-CoV replication and spike-mediated cell-cell fusion, showing great promise for further development into effective viral fusion inhibitors for treating MERS-CoV infection [26, [28] [29] [30] . Among them, the peptide EK1 is effective to multiple human coronaviruses apart from MERS-CoV and therefore serves as a potential pan-coronavirus fusion inhibitor [30] .

Recently, structural studies on the prefusion state spike protein of MERS-CoV and SARS-CoV have provided more insights into the spike-mediated membrane fusion process [31] [32] [33] [34] . The MERS-CoV spike protein trimerizes and folds into a metastable prefusion conformation on the virion surface, in which three S1 domains fold into a steady trimer structure and sit on top to stabilize the coiled S2 domains (Figure 2(A-B) ). We and others have identified that the RBD of SARS-CoV and MERS-CoV can be found either buried ('down' position) or exposed ('up' position) in the spike trimer structure [31, [33] [34] [35] . The two conformational states of RBD may have distinct roles during receptor binding and membrane fusion: only the RBDs in 'up' position, but not those in 'down' position, can bind to the cell receptor DPP4 (Figure 2 (C-D)). Great steric clash was observed between DPP4 and neighboring spike protomers when we mapped it to the RBD in 'down' position ( Figure 2(C-D) ). Transformation of the RBD from the buried to the exposed state is therefore a prerequisite for receptor binding (Figure 2(G-H) ). On the other hand, this conformational change also seems to open up the stable cap structure sitting above the S2 cores ( Figure 2 (E-F)). This may lead to disassociation of S1 trimer and exposure of the fusion apparatus, triggering the membrane fusion process.

To gain a better understanding of MERS-CoV interaction with cellular receptors at atomic levels, we and others have determined the crystal structure of MERS-CoV RBD bound to the extracellular domain of its cellular receptor dipeptidyl peptidase 4 (DPP4) [23, 24] . We showed that MERS-CoV RBD consists of a core and a receptor binding subdomain. MERS-CoV RBD and the related SARS-CoV RBD share a high degree of structural similarity in their core subdomains, but are notably divergent in the receptor binding subdomains [36] . The receptor binding subdomain of MERS-CoV RBD directly interacts with blades 4 and 5 of DPP4 propeller instead of its intrinsic hydrolase domain. The interface consists of a buried surface of ∼2550 Å 2 involving 14 residues in receptor binding subdomain interacting with 15 residues in DPP4. The actual binding forces are mediated through two major binding patches. Patch 1 represents 49% of buried surface and forms between the C-terminal end of the long loop connecting the β6 and β7 strands and blade 4 of DPP4. Patch 2 occupies 51% of buried surface and forms a slightly concaved outer surface at the far end of the MERS-CoV receptor binding subdomain and a linker containing a short helix between blade 4 and blade 5 of DPP4. The concaved outer surface is made by the short β6 strand, C-terminal parts of β5 and β7 strands, N-terminal part of β8 strand and the β5-β6 linking loop. It is hoped that better understanding of the atomic details of the spike glycoprotein, as well as the interface between MERS-CoV RBD and DPP4 will provide the structural basis for rational design and development of therapeutics and vaccines against MERS-CoV infection. 

Neutralizing antibodies are a major component of protective immunity against viral infection in humans. Polyclonal by nature, the antibody response in vivo mobilizes a dynamic and complex mixture of monoclonal antibodies (mAbs) that work in concert to target various antigenic domains on the viral envelope glycoprotein. Identifying the neutralizing mAbs that constitute the neutralizing activity of polyclonal response and their recognized antigenic domains has therefore become the first crucial step towards gaining a better understanding of the protective antibody response, developing clinical intervention methods, and designing immunogens capable of eliciting neutralizing antibodies.

Great achievements have been made in the isolation of neutralizing mAbs in the past few years using various technology platforms ( Figure 3 ). Up till now, more than 20 mAbs, most of which are human or humanized antibodies, have been described by scientists from all over the world. These antibodies are listed in chronological order of publication in Table 1 , together with their unique biochemical and antiviral properties against MERS-CoV infection observed in cell culture and experimental animal models.

It is apparent that the single chain fragment variable (scFv) library approach allows rapid discovery of mAb, without time constraints from immunizing experimental animals or approaching convalescent individuals of MERS-CoV infection. The earliest mAbs reported in 2014 were identified through screening non-immune human scFv libraries with the ectodomain of S glycoprotein (mAb 3B11) [40] or soluble RBD from S glycoprotein (MERS-4, MERS-27 and the m336 panel) [37, 42] as bait protein (Figure 3(A) ). These antibodies all demonstrated high neutralizing activities and therefore were widely used as reference antibodies in later studies.

Antibodies have also been generated from immunized animals (Figure 3 (B)). Several groups have reported mAbs isolated from either wild-type inbred mice or transgenic mice expressing human antibodyvariable heavy chains and κ light chains. Mersmab-1 (known as hMS-1 after humanization) was isolated from mice immunized subcutaneously with chimeric S1-Fc [47, 48] . The mAbs 2E6 and 4C2 (humanized form 4C2 h) were isolated in mice immunized with recombinant RBD produced in insect cells [55] . Furthermore, two human-like mAbs, REGN3048 and REGN3051, were directly cloned from transgenic mice expressing human versions of the antibody after immunization with DNA encoding S glycoprotein and purified recombinant S glycoprotein [51] . Both mAbs have been tested in humanized mice models and in non-human primates [51, 52] . The authors indicated that the advantages of their system not only lay in the human component of their antibodies but also in the quick speed associated with isolation and production, since no humanization or optimization step was required. Currently, REGN3048 and REGN3051 have entered phase I clinical trials.

Most of the mAbs reported so far target the RBD region of S glycoprotein, but RBD does not seem to be the only target for anti-MERS-CoV antibody responses. Recently, a mAb targeting the S1 N-terminus domain (NTD) region, which does not contain RBD, was isolated from mice immunized with S glycoprotein [57] . This antibody, 5F9, was shown to successfully block virus entry in cell culture models and the efficacy was comparable to other mAbs in IC 50 . Further, the mAb panel D12, F11, G2 and G4 were generated by priming Similarly, 3B11 and m336 were isolated from non-immunized human scFv phage libraries with MERS-CoV S protein or RBD protein as bait protein, respectively. (B) Monoclonal antibodies sorted from immunized animals. The antibodies 5F9, hMS-1 (Mersmab-1), D12, F11, G2, G4, 4C2h (4C2), REGN3048 and REGN3051 were isolated from mice immunized with the indicated vaccines labelled in the colour-coded boxes, each representing a different immunogen; the bait or target protein for antibody selection were also listed. The mice from which REGN3048 and REGN3051 were isolated were given the pale blue colour to indicate that they express human immunoglobulin genes. NbMS10-Fc, JC57-11, JC57-13, JC57-14, F1B-H1 and HCAb-83 were isolated from larger animal including llama, rhesus macaque and camels as indicated. The vaccines and selection criteria were also shown. NTD (N-terminal domain), Fc (fragment constant). (C) Monoclonal antibodies isolated from human survivors recovered from MERS-CoV infection. MERS-GD27, MERS-GD33, LCA60, CDC-C2, CDC-C5, CDC-A2 and CDC-A10 were generated by culturing B cells sorted from the patient and screening for MERS-CoV-specific antibodies. MCA1 was produced by constructing a phage library displaying scFv cloned from a convalescent patient. mice with DNA encoding the full-length S glycoprotein and boosting them with S1 protein. Among them are two mAbs that target the non-RBD S1 (mAb G2) and S2 region (mAb G4), respectively [49] . These non-RBD-binding antibodies potently neutralized pseudoand live MERS-CoV in cell culture and were also protective in mouse models [49, 50] . Together, the development of these antibodies elucidates that RBD may not be the single target for anti-viral antibody response. More studies are needed to elaborate the detailed mechanisms for these antibodies.

Apart from the traditional approach of isolating mAbs from immunized mice, several groups have turned to larger animal models for antibody isolation. One group immunized rhesus macaques with combined DNA and protein vaccines and isolated a panel of mAbs, including JC57-11, JC57-13, JC57-14, and FIB-H1, targeting both RBD and non-RBD S1 region of the S glycoprotein, all with potent neutralizing activities [50] . Another group immunized llama with recombinant RBD and screened the nanobody library for high-affinity single heavy chain antibody [61] . These nanobody-derived mAbs are smaller in molecular weight and more stable than traditional antibodies, and may provide a new option for future antibody isolation.

In terms of closeness to authentic human antibodies, no approach can compete with those based on direct B cell cloning from convalescent individuals. One such mAb LCA60 was isolated from memory B cells of human survivors of MERS-CoV infection and was among the most potent mAbs reported in neutralizing pseudo-and live viruses [53] . More mAbs isolated from human survivors were described as more convalescent blood samples became available, including MCA1 [56] , CDC-C2, CDC-C5, CDC-A2, CDC-A10 [50] , MERS-GD27, and MERS-GD33 [58, 59] (Figure  3 (C)), all with potent neutralizing activities against MERS-CoV. The mAbs LCA60, CDC-C2, MCA1, and MERS-GD27 were also tested to be protective in animal models.

As MERS-CoV research progressed quickly in the past few years, many mAbs have been tested for prophylactic or therapeutic protection efficacy in human DPP4 transgenic / transduced mice models, and a few have entered large animal model trials such as in rabbits or non-human primates (NHPs). However, as different animal models were established among labs worldwide with slightly different evaluation end points, it is difficult to make a direct comparison among these mAbs. This is also true for in vitro evaluation of neutralizing activitiessince different cell lines, pseudoviruses, and neutralizing assay techniques are utilized, the published IC 50 values can only serve as indirect reference for comparison. Head to head comparison in the same experimental system would be required to identify the most protective mAb or combination of mAbs against MERS-CoV infection in order to proceed to clinical trials.

We and others have carried out structural studies of MERS-CoV neutralizing antibodies in complex with MERS-RBD to understand neutralizing mechanism at atomic levels ( Figure 4) . Based on the epitopes revealed by structural studies, MERS-CoV antibodies targeting RBD can be classified into three groups (Figure 4(B) , Table 1 ).

The first group consists of antibodies MERS-27, D12, 4C2 and JC57-14, which interact with the Cterminal segment of the β6-β7 loop and β7 strand of RBD by both heavy and light chains (Figure 4(B) ) [38, 49, 50, 55] . Their common epitopes on the RBD include residues Val527, Ser528, Ile529, Val530, Pro531, Ser532, Trp535, Glu536 and Asp539 in the β6-β7 loop. The residues Trp535, Glu536 and Asp539 also happen to be within the DPP4-binding site patch 1 of MERS-CoV RBD [23] , mediating interaction with Lys267 and the carbohydrate moiety linked to Asn229 of DPP4 [38] . Therefore, the Group 1 antibodies would directly compete with DPP4 in binding to RBD by interfering with both protein-protein and protein-carbohydrate interactions between RBD and DPP4. Structural super-impositions also showed that these four antibodies and DPP4 would have steric clashes between the variable domain of the heavy chain and the propeller domain of DPP4 if they simultaneously bind to RBD (Figure 4(C) ).

The second group consists of antibodies m336, MCA1, CDC2-C2 and MERS-GD27, which interact with the β5-β8 strands, β5-β6 loop and β6-β7 loop in RBD mainly by the heavy chain (Figure 4(B) ) [43, 50, 56, 58] . Their common epitope consists of Phe/ Leu506, Asp510, Trp535, Glu536, Asp539, Tyr540, Tyr541, Arg542, and Trp553. Although antibodies in both Group 1 and Group 2 share the binding residues Trp535, Glu536 and Asp539, their approaching angles to the RBD are significantly different. As shown in Figure 4 (C), the approaching angle of Group 2 antibodies is closer to that of DPP4 by rotating approximately 90 degrees anti-clockwise from that of Group 1 antibodies, thereby generating more steric clashes with DPP4. This is also evidenced by a larger overlap between the common epitope of Group 2 antibodies and DPP4-binding site on RBD [23] . As a representative of Group 2 antibodies, m336 exhibits very potent neutralizing activity by not only mimicking critical interactions between RBD and DPP4 but also adopting an approaching angle similar to that of DPP4 (Figure 4(C) ).

The third group consists of antibody MERS-4 and its variant MERS-4V2 with four residue replacements in the HCDR3 (Figure 4(B) ) [39] . By structural determination, it was shown that MERS-4 Fab and MERS-4V2 scFv share the same mode of binding to the RBD (Figure 4(B) ) [39] . Analysis of the RBD/MERS-4V2 complex structure showed that the antibody contacts with the β5-β6, β6-β7 and β7-β8 loops of the receptor-binding subdomain in RBD [39] . The epitope involves Leu507, Ser508, Gln516, Asn519, Asn521, Gln522, Tyr523, Pro525, Lys543, Leu545, and Gly550 [39] . To be note, the MERS-4 epitope has no overlap with DPP4-binding site (Figure 4(C) ). By approaching the RBD outside the DPP4-binding site, MERS-4 recognizes a unique epitope different from all previously reported RBD-targeting antibodies. Comparisons of RBD in DPP4-bound and MERS-4-bound states revealed that binding of MERS-4 induces or fixes the β5-β6 loop into a conformation in which it folds into a shallow groove on the RBD interface critical for accommodating a short helix of DPP4, thereby indirectly disrupting the interaction between RBD and DPP4 (Figure 4(C) ). Such different epitope and mechanism enable MERS-4 to synergize with other antibodies including RBD-targeting MERS-27 and m336 in neutralization, which provides valuable addition for the combined use of antibodies against MERS-CoV infection [39] .

In addition to the aforementioned ten antibodies targeting RBD, the near atomic resolution cryo-EM structures of the trimeric MERS-CoV spike and its complex with antibody G4 were also determined ( Figure 4(D) ) [33] . G4 is the first reported S2-targeting antibody and its epitope consists of a glycosylated, solvent-exposed loop residing in a connector domain between the HR1 and HR2 of the S2 subunit. In the unbound spike trimer structure this loop is largely disordered, whereas it extends out from two β-strands and is surrounded by all six CDRs (complementarity determining regions) of the mAb G4 upon antibody recognition (Figure 4(E) ). The specific spike-G4 interaction may stabilize the loop and further impede conformational changes of S2 subunit essential for membrane fusion after DPP4 binding. The binding epitope for G4 in S2 subunit is more conserved than RBD among MERS-CoV isolates, shedding light on G4 as a potential broad-spectrum neutralizing antibody for MERS-CoV. Yet this loop between HR1 and HR2 is variable in sequence and length among different viruses even in lineage C betacoronaviruses [33] , limiting its application to other coronaviruses. In terms of pan-coronavirus medical countermeasures (MCMs), the recently developed fusion inhibitor peptide EK1 is a potential candidate. The peptide EK1 was designed to target the more conserved HR1 region of the S2 stem, and was shown to block cell-cell fusion induced by spike protein from multiple human coronaviruses [30] .

In general, most reported MERS-CoV neutralizing antibodies recognize the RBD in the S1 subunit, and these antibodies are highly potent in neutralization. These facts show that the RBD in the S1 subunit is a major vulnerable site for antibody recognition and neutralization. To be note, the RBD is also the region where most naturally occurring mutations of the S glycoprotein occur. Currently, the comprehensively studied antibodies targeting the non-RBD region of the spike glycoprotein also include mAb 5F9 targeting the N-terminal domain (NTD) of the S1 subunit [57] , as well as mAbs G2, CDC2-A2, CDC2-A10, JC57-13 and FIB-H1 targeting the non-RBD region of the S1 subunit [33, 50] . However, the detailed epitopes and specific mechanisms are still unclear for these antibodies. We expect that more antibodies with new neutralizing epitopes and/or mechanisms would be important for the combined use of antibodies against MERS-CoV infection.

Although monoclonal antibodies show promising antiviral effects in both cell culture and animal models against MERS-CoV infection, their roles are still limited in large-scale disease prevention in MERS-CoV high risk areas, as the therapeutic window is generally narrow for mAbs and mass-scale production is timeand resource-consuming. Vaccines still remain the best choice for MERS-CoV prevention.

Given its critical role in mediating viral entry and as major targets for neutralizing antibodies, S glycoprotein and its RBD have become the prime targets for MERS-CoV immunogen design and vaccine development. Various approaches have been applied and more than twenty vaccine candidates have been reported in the past few years, including vaccines based on inactivated virions [62, 63] , virus-like particles [64] , recombinant viral vectors [65] [66] [67] [68] [69] [70] [71] [72] [73] [74] [75] [76] [77] [78] [79] [80] , DNA [49, 81, 82] , recombinant protein subunits [33, 49, [83] [84] [85] [86] [87] [88] [89] [90] [91] [92] , and nanoparticles [80, 93, 94] . Table 2 summarizes the critical features of these approaches and their protective potentials in experimental animal models.

Up till now, only two vaccine candidates, GLS-5300 and MERS001, have entered human clinical trials. The vaccine GLS-5300 was the first to be tested in healthy human volunteers. It is a DNA plasmid encoding the MERS-CoV S glycoprotein, requiring two-to-three injections delivered by electroporation [81] . The phase I clinical trial was started in 2016 at the Walter Reed Army Institute, and another phase I/II clinical trial is being conducted in Korea to test dosage safety and immunogenicity. Another vaccine candidate, MERS001, is a replication-deficient chimpanzee adenovirus (ChAdOx1) containing the MERS-CoV S glycoprotein antigen [70, 71] . This vaccine only requires one-time administration of 5×10 9 -5×10 10 virus particles via intramuscular route, and the local adverse events as well as immunogenicity will be evaluated in the phase I clinical trial conducted at the University of Oxford. In addition, one more candidate vaccine has been tested in dromedary camels either for potential human use or straight into veterinary use. It explores a modified vaccinia virus Ankara (MVA) as a vector to express MERS-CoV S glycoprotein [67] . The regimen involves immunization through intranasal as well as intramuscular routes twice at a 4-week interval. The vaccinated camels demonstrated a significant reduction of excreted infectious virus and viral RNA transcripts in vaccinated animals upon MERS-CoV challenge. Protection against MERS-CoV infection correlated with the presence of serum neutralizing antibodies to MERS-CoV. As MVA has established a reasonably good safety profile in humans and induced desirable protective immunity in camels, it represents one of the potential candidates to be further evaluated in humans in the near future.

The remaining vaccine candidates are all in the stages of preclinical or laboratory development and invariably target the S glycoprotein or RBD critical for viral entry ( Table 2) . Vaccines based on inactivated [62, 63] or virus-like particles [64] have historical precedence in inducing protective immune responses in humans. Whether the same strategies are applicable to MERS-CoV requires further studies, particularly when it comes to possible safety concerns [62] .

Apart from MERS001 and the MVA-based vaccine tested in dromedary camels, other vector-based approaches are also being actively pursued, including adenovirus [68, 69, 72, 73, 80] , measles virus [74, 75] , VEEV replicon particle [76, 77] , vesicular stomatitis virus [78] , and rabies virus [79] . All recombinant viruses encoding the MERS-CoV S or S1 antigen demonstrated strong immunogenicity in mice or non-human primate models, and some were shown to confer protection in MERS-CoV challenge mouse models (Table 2) . However, concerns remain regarding the pre-existing immunity against these viral vectors from natural infection, because it would diminish the vaccine potency [95] . To overcome the issue of preexisting immunity against human adenoviruses while preserving their advantages such as high yields and strong immunogenicity, rare serotypes of chimpanzee adenovirus of low human seroprevalence may be adopted as viral vectors [70, 73] . Our group recently developed a vaccine candidate with replication-defective chimpanzee adenovirus C68 (AdC68) vector expressing full length MERS-CoV S glycoprotein. Seroprevalence of AdC68 is around 2% in human population, much lower than that of the commonly used human adenovirus 5 (HuAd5) vector (>60%) [96, 97] . One intra-nasal administration of 2 × 10 9 viral particles completely protected human DPP4 knock-in (hDPP4-KI) mice from lethal MERS-CoV challenge, and passive transfer of AdC68-S immune sera conferred survival advantage in lethal challenge mouse models [73] . Further, the safety profiles of these vectors have yet to be extensively tested in humans. Recently, Hashem and colleagues showed that the adenovirus-based S1 vaccine may pose potential safety concerns because it may induce pulmonary perivascular hemorrhage in a MERS-CoV challenge mouse model, regardless of the its full protection upon lethal viral infection. They also showed that the pulmonary pathology can be mitigated by incorporating CD40L, an immune-modulator therefore potential molecular adjuvant, into the recombinant adenovirus-based vaccine [72] . Whether this vaccine-associated pathology is related to residual infectious viruses or unbalanced immune responses awaits further investigation. With this in mind, all future MERS-CoV vaccine candidate designs should take extra cautions on safety evaluation. Furthermore, recombinant-protein-based vaccines are widely pursued. Strategies to solubilize the MERS-CoV S glycoprotein in order to form stable immunogens include forming nanoparticles and using soluble protein truncations. In particular, both nanoparticles formed with full length MERS-CoV S glycoprotein [93, 94] and subunit RBD-based vaccines [83] [84] [85] [86] [87] [88] [89] [90] have been shown to induce virus neutralizing antibodies and to protect mice when challenged with MERS-CoV. One RBD subunit vaccine also conferred protection in rhesus macaques [91] . This indicates that RBD alone as antigen may be sufficient for protective immunity to develop against the virus. Along with the finding that mAb targeting NTD is able to neutralize MERS-CoV, Lan et al showed that three doses of intramuscularly administered recombinant NTD protein also induced protective immunity against live MERS-CoV in human DPP4 transduced mouse model (Ad5-hDPP4 mice) [92] . More recently, with the structural insights into the spike glycoprotein, Pallesen et al developed a prefusion-stabilized S trimer vaccine by substituting proline residues into the S2 domain [33] . The introduction of proline disfavours the refolding of the linker between HR1 and the central helix, thus preventing the transition of spike into the post-fusion state. This rationally designed antigen, MERS S-2P, was shown to induce broader and more potent neutralizing activity than wild type spike trimer protein [33] .

Finally, a prime-boost strategy based on a full-length S glycoprotein DNA vaccine followed by an S1-glycoprotein boost was able to induce virus-neutralizing antibodies and confer protection against the clinical severity of diseases in non-human primate models [49] . Compared with the protein-only regimen, the combination of DNA and protein induced a more functionally diverse antibody repertoire and stronger Th1 immune response. It was suggested that the native S glycoprotein conformation, formed on the cell surface after DNA vaccination, helped induce more diverse antibodies against MERS-CoV.

As summarized in Table 2 , most of the aforementioned strategies require multiple immunizations which may pose additional logistic hurdles at the end point use. It is unclear whether these immunization strategies were empirically designed or due to relatively # Abbreviations for animal models: human DPP4 transgenic (hDPP4-Tg) mice with global/epithelial hDPP4 expression, human DPP4 knock-in (hDPP4-KI) mice with hDPP4 replacing mDPP4 in situ, mice transduced with human adenovirus 5 vector expressing hDPP4 (Ad5-hDPP4 mice), and non-human primates (NHPs).

* Efficacy in the specific animal model listed in the previous column. If studies are conducted in multi-animal models, only results in the highest-level model are shown. Neutralizing antibody (nAb).

↑ indicates more, while ↓ indicates less.

poor immunogenicity of candidate vaccines. For practical and compliant purposes, a single immunization with the highest immunogenicity in animals and humans will be preferred.

The outbreak of MERS-CoV in Saudi Arabia in 2012 reminded us of the 2003 SARS-CoV outbreak in China. Despite the differences in geographic location, epidemiology and immediate animal reservoirs, these two viruses share remarkable similarity in causing severe respiratory syndrome, leading to high fatality in humans and trigger serious public health concerns. With the advent of modern techniques in virology, immunology and vaccinology, we have gained substantial insights into the biology of MERS-CoV, and its pathogenesis with unprecedented speed and accuracy. As summarized in the current review, tremendous progress has been made in understanding (1) the entry process of MERS-CoV into target cells, (2) the structure and function of S glycoprotein and cellular receptor DPP4 in mediating viral entry, (3) antibody response during natural infection and isolation of broad and potent neutralizing mAbs, and (4) design and development of vaccine candidates using various innovative technologies. However, our progress in translating these discoveries into clinical application has been slow. Only two vaccine candidates and one mAb panel have entered phase I clinical trials for safety. Ironically, no vaccines and treatment strategies have been approved for SARS-CoV infection even after more than a decade of outbreak. We could not imagine how catastrophic it would be should SARS-CoV hit again or MERS-CoV continues to probe and gain strong capacity in transmission to and among humans.

We are facing a difficult predicament when it comes to public health challenges in the new era of emerging and re-emerging infectious diseases. On one hand, the human population is becoming ever mobile and exposed to an increasing number of pathogens. On the other hand, translating basic discoveries into preventative and treatment applications has been exceedingly slow. Among many plausible reasons, a lack of incentives in financial returns perhaps stands the tallest. The deadlock is not just happening to MERS-CoV and SARS-CoV but also to many other infectious pathogens such as Ebola, Marburg, Lassa, highly pathogenic avian influenza, HIV-1, and so on. Fundamental and drastic changes have to be made in the entire research and development system before we can truly prepare and position ourselves ahead of deadly epidemic and pandemic. Only then, can our speed and accuracy in basic discovery be timely translated into clinical and public health needs. The time to act is now.

Newly discovered coronavirus as the primary cause of severe acute respiratory syndrome

Epidemiology and cause of severe acute respiratory syndrome (SARS) in

People's Republic of China

A new virus isolated from the human respiratory tract

Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease

Identification of a new human coronavirus

Characterization and complete genome sequence of a novel coronavirus, coronavirus HKU1, from patients with pneumonia

Summary of probable SARS cases with onset of illness from 1

Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia

Middle East respiratory syndrome coronavirus (MERS-CoV

Middle East respiratory syndrome

Origin and evolution of pathogenic coronaviruses

Genetic relatedness of the novel human group C betacoronavirus to Tylonycteris bat coronavirus HKU4 and Pipistrellus bat coronavirus HKU5. Emerg Microbes Infect

Genomic characterization of a newly discovered coronavirus associated with acute respiratory distress syndrome in humans

Rooting the phylogenetic tree of middle east respiratory syndrome coronavirus by characterization of a conspecific virus from an African Bat

Further evidence for bats as the evolutionary source of Middle East respiratory syndrome coronavirus

Bat origins of MERS-CoV supported by bat coronavirus HKU4 usage of human receptor CD26

Origins and pathogenesis of Middle East respiratory syndrome-associated coronavirus: recent advances

Middle East respiratory syndrome coronavirus (MERS-CoV): announcement of the coronavirus study group

Role of the spike glycoprotein of human Middle East respiratory syndrome coronavirus (MERS-CoV) in virus entry and syncytia formation

Coronavirus spike proteins in viral entry and pathogenesis

The spike protein of SARS-CoV -a target for vaccine and therapeutic development

PubMed Central PMCID: PMCPmc2750777; eng

Structure of MERS-CoV spike receptor-binding domain complexed with human receptor DPP4

Molecular basis of binding between novel human coronavirus MERS-CoV and its receptor CD26

The receptor binding domain of the New Middle East respiratory syndrome coronavirus Maps to a 231-residue region in the spike protein that efficiently elicits neutralizing antibodies

Structure of the fusion core and inhibition of fusion by a heptad repeat peptide derived from the S protein of Middle East respiratory syndrome coronavirus

Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC

Structure-based discovery of Middle East respiratory syndrome coronavirus fusion inhibitor

Combining a fusion inhibitory peptide targeting the MERS-CoV S2 protein HR1 domain and a neutralizing antibody specific for the S1 protein receptor-binding domain (RBD) showed potent Synergism against pseudotyped MERS-CoV with or without mutations in RBD. Viruses

A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike. Sci Adv

Cryo-electron microscopy structures of the SARS-CoV spike glycoprotein reveal a prerequisite conformational state for receptor binding

PubMed Central PMCID: PMCPMC5223232; eng

Stabilized coronavirus spikes are resistant to conformational changes induced by receptor recognition or proteolysis

Immunogenicity and structures of a rationally designed prefusion MERS-CoV spike antigen

Cryo-EM structures of MERS-CoV and SARS-CoV spike glycoproteins reveal the dynamic receptor binding domains

Cryo-EM structure of the SARS coronavirus spike glycoprotein in complex with its host cell receptor ACE2

Receptor recognition mechanisms of coronaviruses: a decade of structural studies

Potent neutralization of MERS-CoV by human neutralizing monoclonal antibodies to the viral spike glycoprotein

Structural basis for the neutralization of MERS-CoV by a human monoclonal antibody MERS-27

1038/srep13133. PubMed PMID: 26281793; PubMed Central PMCID: PMCPmc4539535; eng

Structural definition of a unique neutralization epitope on the receptor-binding domain of MERS-CoV spike glycoprotein

Identification of human neutralizing antibodies against MERS-CoV and their role in virus adaptive evolution

PubMed Central PMCID: PMCPmc4024880; eng

3B11-N, a monoclonal antibody against MERS-CoV, reduces lung pathology in rhesus monkeys following intratracheal inoculation of MERS-CoV Jordan-n3/2012

Exceptionally potent neutralization of Middle East respiratory syndrome coronavirus by human monoclonal antibodies

Junctional and allelespecific residues are critical for MERS-CoV neutralization by an exceptionally potent germline-like antibody

Efficacy of antibody-based therapies against Middle East respiratory syndrome coronavirus (MERS-CoV) in common marmosets

Prophylaxis With a Middle East respiratory syndrome coronavirus (MERS-CoV)-specific human monoclonal antibody Protects rabbits from MERS-CoV infection

Passive transfer of A Germline-like neutralizing human monoclonal antibody Protects transgenic mice against lethal Middle East respiratory syndrome coronavirus infection. Sci Rep

A conformation-dependent neutralizing monoclonal antibody specifically targeting receptor-binding domain in Middle East respiratory syndrome coronavirus spike protein

Single-dose treatment with a humanized neutralizing antibody affords full protection of a human transgenic mouse model from lethal Middle East respiratory syndrome (MERS)-coronavirus infection

Evaluation of candidate vaccine approaches for MERS-CoV

Importance of neutralizing monoclonal antibodies targeting multiple antigenic sites on MERS-CoV spike to avoid neutralization escape

Pre-and postexposure efficacy of fully human antibodies against spike protein in a novel humanized mouse model of MERS-CoV infection

Prophylactic and therapeutic efficacy of mAb treatment against MERS-CoV in common marmosets

Prophylactic and postexposure efficacy of a potent human monoclonal antibody against MERS coronavirus

PubMed Central PMCID: PMCPmc4547275; eng

Prophylactic efficacy of a human monoclonal antibody against MERS-CoV in the common marmoset

A humanized neutralizing antibody against MERS-CoV targeting the receptorbinding domain of the spike protein

Human neutralizing monoclonal antibody inhibition of Middle East respiratory syndrome coronavirus replication in the common marmoset

A novel neutralizing monoclonal antibody targeting the N-terminal domain of the MERS-CoV spike protein. Emerg Microbes Infect

Ultra-potent human neutralizing antibody Repertoires against MERS-CoV from A recovered patient

A novel human mAb (MERS-GD27) provides prophylactic and postexposure efficacy in MERS-CoV susceptible mice. Sci China Life Sci

A novel nanobody targeting Middle East respiratory syndrome coronavirus (MERS-CoV) receptor-binding domain has potent cross-neutralizing activity and protective efficacy against MERS-CoV

Chimeric camel/human heavy-chain antibodies protect against MERS-CoV infection

Immunization with inactivated Middle East respiratory syndrome coronavirus vaccine leads to lung immunopathology on challenge with live virus

Enhanced protection in mice induced by immunization with inactivated whole viruses compare to spike protein of Middle East respiratory syndrome coronavirus

MERS-CoV virus-like particles produced in insect cells induce specific humoural and cellular imminity in rhesus macaques

Middle East respiratory syndrome coronavirus spike protein delivered by modified Vaccinia virus Ankara efficiently induces virus-neutralizing antibodies

Protective efficacy of recombinant modified Vaccinia virus Ankara delivering Middle East respiratory syndrome coronavirus spike glycoprotein

An orthopoxvirus-based vaccine reduces virus excretion after MERS-CoV infection in dromedary camels

Immunogenicity of an adenoviral-based Middle East respiratory syndrome coronavirus vaccine in BALB/c mice. Vaccine

Systemic and mucosal immunity in mice elicited by a single immunization with human adenovirus type 5 or 41 vector-based vaccines carrying the spike protein of Middle East respiratory syndrome coronavirus

Protective efficacy of a novel simian adenovirus vaccine against lethal MERS-CoV challenge in a transgenic human DPP4 mouse model. NPJ Vaccines

Chadox1 and MVA based vaccine candidates against MERS-CoV elicit neutralising antibodies and cellular immune responses in mice

A highly immunogenic, protective and safe adenovirus-based vaccine expressing MERS-CoV S1-CD40L fusion protein in transgenic human DPP4 mouse model

Single intranasal immunization with chimpanzee adenovirusbased vaccine induces sustained and protective immunity against MERS-CoV infection. Emerg Microbes Infect

A highly Immunogenic and protective Middle East respiratory syndrome coronavirus vaccine based on a recombinant measles virus vaccine platform

Live-attenuated bivalent measles virus-derived vaccines targeting Middle East respiratory syndrome coronavirus induce robust and multifunctional T cell responses against both viruses in an appropriate mouse model. Virology

Rapid generation of a mouse model for Middle East respiratory syndrome

Middle East respiratory syndrome coronavirus Causes multiple Organ Damage and lethal disease in mice transgenic for human dipeptidyl peptidase 4

A recombinant VSV-vectored MERS-CoV vaccine induces neutralizing antibody and T cell responses in rhesus monkeys after single dose immunization

One-health: a safe, efficient, dual-use vaccine for humans and animals against Middle East respiratory syndrome coronavirus and rabies virus

Heterologous prime-boost vaccination with adenoviral vector and protein nanoparticles induces both Th1 and Th2 responses against Middle East respiratory syndrome coronavirus. Vaccine

A synthetic consensus anti-spike protein DNA vaccine induces protective immunity against Middle East respiratory syndrome coronavirus in nonhuman primates

DNA vaccine encoding Middle East respiratory syndrome coronavirus S1 protein induces protective immune responses in mice

A Truncated receptor-binding domain of MERS-CoV spike protein potently Inhibits MERS-CoV infection and induces strong neutralizing antibody responses: Implication for developing therapeutics and vaccines. PloS one

Searching for an ideal vaccine candidate among different MERS coronavirus receptor-binding fragments -the importance of immunofocusing in subunit vaccine design. Vaccine

Identification of an ideal adjuvant for receptor-binding domain-based subunit vaccines against Middle East respiratory syndrome coronavirus

Optimization of antigen dose for a receptor-binding domain-based subunit vaccine against MERS coronavirus

Receptor-binding domain of MERS-CoV with optimal immunogen dosage and immunization interval protects human transgenic mice from MERS-CoV infection

A recombinant receptorbinding domain of MERS-CoV in trimeric form protects human dipeptidyl peptidase 4 (hDPP4) transgenic mice from MERS-CoV infection

Intranasal vaccination with recombinant receptor-binding domain of MERS-CoV spike protein induces much stronger local mucosal immune responses than subcutaneous immunization: Implication for designing novel mucosal MERS vaccines. Vaccine

Tailoring subunit vaccine immunity with adjuvant combinations and delivery routes using the Middle East respiratory coronavirus (MERS-CoV) receptor-binding domain as an antigen

Recombinant receptor binding domain protein induces Partial protective Immunity in rhesus macaques against Middle East respiratory syndrome coronavirus challenge

The recombinant N-terminal domain of spike proteins is a potential vaccine against Middle East respiratory syndrome coronavirus (MERS-CoV) infection. Vaccine

Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice. Vaccine

MERS-CoV spike nanoparticles protect mice from MERS-CoV infection

Effect of preexisting immunity to adenovirus human serotype 5 antigens on the immune responses of nonhuman primates to vaccine regimens based on human-or chimpanzee-derived adenovirus vectors

International epidemiology of human pre-existing adenovirus (Ad) type-5, type-6, type-26 and type-36 neutralizing antibodies: correlates of high Ad5 titers and implications for potential HIV vaccine trials. Vaccine

Replication-defective vector based on a chimpanzee adenovirus

No potential conflict of interest was reported by the authors.

Jiuyang Xu http://orcid.org/0000-0002-1906-5918 Xinquan Wang http://orcid.org/0000-0003-3136-8070 Linqi Zhang http://orcid.org/0000-0003-4931-509X