key: cord-1030124-xfjbkriz authors: Loffredo, Lorenzo; Fallarino, Alessia; Paraninfi, Aurora; Pacella, Fernanda; Pacella, Elena; Oliva, Alessandra; Violi, Francesco title: Different rates of conjunctivitis in COVID-19 eastern and western hospitalized patients: a meta-analysis date: 2021-11-09 journal: Intern Emerg Med DOI: 10.1007/s11739-021-02880-z sha: e687a1342a0d7d599e3118f97987dbf957a8f1f6 doc_id: 1030124 cord_uid: xfjbkriz nan The coronavirus disease 2019 (COVID-19) is a lung infection that in its most severe manifestations leads to ischemic, thrombotic and respiratory complications that can increase mortality and intensive care admission [1] . In the early phase of pandemic infection, we assessed the relationship between conjunctivitis and COVID-19 [2] . Interestingly, we found an association between COVID-19 severe disease and an increased incidence of conjunctivitis [2] . However, the meta-analysis was limited by the low rate of events and by the population composed exclusively by Chinese COVID-19 patients. Thus, after almost 2 years of pandemic, the goal of this meta-analysis is to assess whether the association with COVID-19 severity persists in a larger number of patients with conjunctivitis and different ethnicities. As eligibility criteria we assessed the following types of studies: clinical studies in hospitalized patients with COVID-19 infection that assessed the severe (as severe pneumonia, mortality, ARDS, and use of mechanical ventilation or ICU recovery) and non-severe forms of the disease and reported the incidence of conjunctivitis. No publication date, or publication status restrictions were imposed. Only publications written in English language were included in the meta-analysis. The studies were identified by searching electronic databases. This search was applied to Pubmed, ISI Web of Science, SCOPUS and Cochrane database. The last search was run on July 28th, 2021. Reference lists of all studies included in the present meta-analysis were screened for potential additional eligible studies. Two investigators (A.F. and L.L.) independently searched in the electronic databases combining the following text terms and MeSH terms: "COVID-19"[All Fields] OR "severe acute respiratory syndrome coronavirus 2"[All Fields] OR "severe acute respiratory syndrome coronavirus 2"[All Fields] OR "2019-nCoV"[All Fields] OR "SARS-CoV-2"[All Fields] AND ("conjunctivitis"[MeSH Terms] OR "eye"[All Fields] OR ADULTS[All Fields]). Two authors (L.L., A.F.) independently reviewed titles and abstracts generated by search. Studies were excluded if the title and/or abstract showed that the papers did not meet the selection criteria of our meta-analysis. For potentially eligible studies or if the relevance of an article could not be excluded with certitude, we procured the full text. Disagreements were resolved by discussion between L.L. and A.F.; if no agreement was reached, a third author (A.P.) decided. Studies not including a control group and animal studies were excluded. Case reports, editorials, commentaries, letters, review articles, and guidelines were also excluded from the analysis (Fig. 1) . We evaluated the frequency of conjunctivitis in hospitalized patients affected by severe and non-severe COVID-19 infection. This review was conducted and reported according to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analysis). We allocated the results of each trial as dichotomous frequency data. We considered a p value < 0.05 as significant. Odds Ratios (OR) and 95% confidence intervals (CIs) were calculated. Data were pooled and compared with a randomeffect model. The presence of publication bias was evaluated using the Egger's test. Statistical heterogeneity was calculated by I 2 . The software Comprehensive Meta-Analysis (version 2.2.064, USA, 2011) supported the analysis. The presence of publication bias was explored using funnel plots of effect size against standard error. We included studies in hospitalized patients with COVID-19 that assessed the severe (as severe pneumonia, mortality, ARDS, use of mechanical ventilation or intensive care units recovery) and non-severe forms of the disease and reported the incidence of conjunctivitis. Eleven studies [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] including 2308 patients, assessed the clinical course of patients with COVID-19 and presence/ absence of conjunctivitis (Fig. 1) . Clinical characteristics of the studies are reported in Table 1 . Patients from Euro-Mediterranean area (Italy, Germany, Spain, Turkey, Egypt) and Asia (China) were included (Table 1) . Compared to Asian studies, a higher incidence of conjunctivitis was observed in those of Euro-Mediterranean area (severe COVID: 9% vs 16% and not-severe: 1.3 vs 7%, respectively). A significant increase of conjunctivitis in severe COVID-19 patients was observed in both ethnic groups (random model, Asian population: O.R. 2.5; 95% C.I. 1.1-6.0; p = 0.03; Euro-Mediterranean population: O.R. 2.3; 95% C.I. 1.1-4.9; p = 0.02) (Fig. 2) . The overall rate of conjunctivitis was 5.9%; it was 12.7% and 4.1% in severe and non-severe COVID-19 patients, respectively. The overall meta-analysis showed that patients with severe COVID-19 infection had an increased incidence of conjunctivitis (random model, O.R. 2.4; 95% C.I. 1.4-4; p = 0.002) (Fig. 2) . Moderate heterogeneity among trials was observed (I 2 = 32%); the publication bias was not statistically significant (Egger's test, p = 0.395). This meta-analysis provides evidence that conjunctivitis, in a multi-ethnic population, is associated with a more severe form of COVID-19; this difference remains also when the subgroups analysis for ethnic groups was performed. The close relationship between conjunctivitis and COVID severity could be very important for clinicians to recognize a possible critical evolution of the patient early. In this regard, we recently demonstrated [13] in patients admitted to the medical wards for COVID pneumonia that when conjunctivitis appears the prognosis becomes poor with an increase of intensive care admissions or mortality rate. An interesting hypothesis is if conjunctivitis is related not to the route of entry of the virus in the human body but to the manifestation of a systemic disease. Thus, conjunctivitis could represent a sign of a systemic disease and a warning sign of poor outcome consequent to the systemic inflammation. This hypothesis may be supported by the multisystem inflammatory syndrome in young with COVID-19, where conjunctivitis has been described as a sign of a storm of cytokines and inflammatory molecules [14, 15] . Another important message that comes from this metaanalysis is the high incidence (approximately 13%) of conjunctivitis in multi-ethnic patients with severe COVID. Although a higher incidence of conjunctivitis has been observed in the Euro-Mediterranean studies, no definitive conclusions can be drawn as further targeted studies, aimed at comparing this ocular sign in the European and Asian COVID population with a large sample and with similar clinical features, should be done. In any case, this meta-analysis shows that conjunctivitis is a frequent manifestation in both COVID areas and that this clinical sign should be carefully considered for its poor prognosis. This meta-analysis has the following limitations: -Composite events were analyzed; there are currently few studies [8, 13] that analyzed single events, such as mortality and ICU admission. Future studies need to evaluate the association among these single outcomes, COVID and conjunctivitis. -The prognostic value of the conjunctivitis cannot be fully elucidated. We cannot exclude that conjunctivitis occurred following environments that favored its appearance such as that of intensive care. The predictive role of conjunctivitis was analyzed in an our previous study [13] in which the diagnosis was done at admission and we found an association of conjunctivitis with increased mortality and ICU admissions. However, further longitudinal studies are needed to confirm this association. -The Asian studies included in this meta-analysis were performed in the first phase of the pandemic period (by February 2020) unlike the European ones performed in a later phase (after February 2020); this could have created a different infectious scenario due to the increase in COVID-19 variants and a different incidence among the populations studied. In conclusion, the results of this meta-analysis show that conjunctivitis may represent a sign of severe COVID-19 infection associated with a poor outcome in Asian and Euro-Mediterranean patients. Funding All the authors have no financial relationships relevant to this article to disclose. Conflict of interest All the authors have no conflicts of interest to disclose. Human and animal rights This article does not contain any studies with human participants or animals performed by any of the authors. Informed consent For this type of study formal consent is not required. 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