key: cord-1028745-kwzvp4v0 authors: Dutta, Noton K.; Mazumdar, Kaushiki; Gordy, James T. title: The Nucleocapsid Protein of SARS–CoV-2: a Target for Vaccine Development date: 2020-06-16 journal: J Virol DOI: 10.1128/jvi.00647-20 sha: 1602207e3d46ac630bf0bd9bbed083f1b2b00635 doc_id: 1028745 cord_uid: kwzvp4v0 nan knowledge of its genetics and biochemistry, and its strong immunogenicity, the N protein of SARS-CoV-2 should be strongly considered as a vaccine candidate for SARS-CoV-2. A pneumonia outbreak associated with a new coronavirus of probable bat origin The genome sequence of the SARS-associated coronavirus Identification of a novel coronavirus in patients with severe acute respiratory syndrome Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation Structure, function, and antigenicity of the SARS-CoV-2 spike glycoprotein Structural modeling of 2019-novel coronavirus (nCoV) spike protein reveals a proteolyticallysensitive activation loop as a distinguishing feature compared to SARS-CoV and related SARS-like coronaviruses Crystal structure of SARS-CoV-2 nucleocapsid protein RNA binding domain reveals potential unique drug targeting sites Potential rapid diagnostics, vaccine and therapeutics for 2019 novel coronavirus (2019-nCoV): a systematic review The SARS-CoV-2 vaccine pipeline: an overview HIV-1 evades antibody-mediated neutralization through conformational masking of receptor-binding sites A sequence homology and bioinformatic approach can predict candidate targets for immune responses to SARS-CoV-2 Comparative full-length genome sequence analysis of 14 SARS coronavirus isolates and common mutations associated with putative origins of infection Evasion of antibody neutralization in emerging severe acute respiratory syndrome coronaviruses Virology. The SARS coronavirus: a postgenomic era Characterization of a novel coronavirus associated with severe acute respiratory syndrome Isolation of virus from a SARS patient and genome-wide analysis of genetic mutations related to pathogenesis and epidemiology from 47 SARS-CoV isolates Nucleocapsid protein recruitment to replication-transcription complexes plays a crucial role in coronaviral life cycle Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid Effects of a SARS-associated coronavirus vaccine in monkeys The development of vaccines against SARS corona virus in mice and SCID-PBL/hu mice Detection of antibodies against SARS-coronavirus using recombinant truncated nucleocapsid proteins by ELISA Search for potential target site of nucleocapsid gene for the design of an epitope-based SARS DNA vaccine Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43 Analysis of preferred codon usage in the coronavirus N genes and their implications for genome evolution and vaccine design The SARS coronavirus nucleocapsid protein-forms and functions We received no funding. We report no conflicts of interest. We have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest.