key: cord-1018634-bq8xl59x
authors: Habibzadeh, Parham; Sajadi, Mohammad M.; Emami, Amir; Karimi, Mohammad Hossein; Yadollahie, Mahboobeh; Kucheki, Maryam; Akbarpoor, Sahar; Habibzadeh, Farrokh
title: Rate of re-positive RT-PCR test among patients recovered from COVID-19
date: 2020-08-05
journal: Biochem Med (Zagreb)
DOI: 10.11613/bm.2020.030401
sha: 5c0552f6bcc16a5d35a1c15eebf0de969fd5d34d
doc_id: 1018634
cord_uid: bq8xl59x

nan

Recently, health care officials in Larestan (Iran) decided to use the convalescent plasma to treat newly infected patients. They asked 35 patients with confirmed COVID-19 who had been hospitalized in Larestan Hospital during March 22 to March 26, 2020, to donate plasma. The diagnosis was made by RT-PCR according to a method described earlier (5) . The RNA was extracted from nasopharyngeal swabs (Invitrogen ChargeSwitch Total RNA Cell Kit, Invitrogen Co, USA). The assay tested the E and RdRP genes (5) . Both internal and negative controls were performed for tests. Thirteen of the 35 invited patients did agree to donate. They were thus re-tested with RT-PCR (using the same method) to determine if they were RT-PCR-negative.

The study protocol was approved by the Petroleum Industry Health Organization Institutional Review Board.

Reverse transcription polymerase chain reaction test was positive in 9 (5 male, 4 female) of 13 recovered patients. They had a median age of 52 (interquartile range (IQR) 30 to 56) years. They presented mostly with cough, fever, malaise, and dyspnoea. On admission, 6 had abnormal findings in their chest and mediastinal unenhanced spiral computed tomography, including ground-glass opacities with or without consolidation; 4 had a positive C-reactive protein test; and 5 had 1 or 2 underlying medical conditions, most commonly hypertension (4 of 5). The disease severity was mild to moderate. The patients were hospitalized for a median of 5 (IQR 3 to 10) days. During hospitalization, they received osteltamivir (75 mg taken orally every 12 hours) and/or lopinavir/ritonavir (400/100 mg taken orally every 12 hours). They were discharged home when their symptoms resolved completely. Other laboratory parameters measured at the time of admission were within normal limits ( Table 1 ). The second RT-PCR test was found positive in these patients after a medi- Observing 9 positive RT-PCR tests in 13 recovered patients after a median of 18 days of complete resolution of their symptoms -a positive rate of almost 70% -is very high. Even if we assume that all the remaining invited (but unattended) 22 recovered patients would have tested negative, the rate was still high -26% (95% confidence interval (CI): 11% to 41%). Considering the low sensitivity (high false-negative rate) of RT-PCR test for the diagnosis of COVID-19 in nasopharyngeal samples, this rate would clearly be an underestimation (6) . On account of the low sample size studied, the results might be considered trivial, at the first glance; however, even the lower limit of the 95% CI of the rate, 11%, is unacceptably high and potentially dangerous.

This high RT-PCR re-positive rate would have serious health implications in the world and might even change our strategies to tackle with the current pandemic. These patients, although asymptomatic, can potentially spread the virus after more than 2 weeks (even 48 days) of complete resolution of their symptoms. It is, however, worth to note that RT-PCR does not discriminate between intact whole virus and viral RNA. Therefore, a positive test does not necessarily imply an active infection or ability to transmit infection. This underlines the importance of developing tests to detect active viral replication and employing an active surveillance for identifying those infected with the virus, even asymptomatic people.

None declared. 

The Novel Coronavirus: A Bird's Eye View

Testing an Old Therapy Against a New Disease: Convalescent Plasma for COVID-19

Positive RT-PCR Test Results in Patients Recovered From COVID-19

Findings from investigation and analysis of re-positive cases 2020

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

Variation in False-Negative Rate of Reverse Transcriptase Polymerase Chain Reaction-Based SARS-CoV-2 Tests by Time Since Exposure