key: cord-1014748-sbrznhdn
authors: Slots, Jørgen
title: Herpesviral–bacterial interactions in periodontal diseases
date: 2009-12-11
journal: Periodontol 2000
DOI: 10.1111/j.1600-0757.2009.00308.x
sha: 662449ff6eb17541b354a4fdb639edfec5b6bf25
doc_id: 1014748
cord_uid: sbrznhdn

nan

Periodontitis is one of the most complex infectious diseases of the human body. Individual periodontal lesions may harbor millions of genomic copies of herpesviruses (179) as well as papillomaviruses, human immunodeficiency virus (HIV), human T-lymphotropic virus type 1, torquetenovirus, and hepatitis B and C viruses (190) . Herpesvirus-infected periodontal sites tend to exhibit more breakdown than herpesvirus-free sites, and a herpesviral active infection is associated with an elevated risk of progressive periodontal disease (189) . Furthermore, the oral cavity supports more than 700 bacterial species, and the periodontal pocket area harbors more than 400 bacterial species (148) . Periodontopathic bacteria, such as Porphyromonas gingivalis and Tannerella forsythia, possess virulence factors involved in colonizing periodontal sites, neutralizing local host defenses and destroying periodontal tissues (78, 192) .

The host immune response attempts to control both pathogenic viruses and bacteria in periodontal sites. However, it is unclear if various immune mediators, such as certain cytokines and chemokines, exert primarily a protective or a destructive role in periodontal disease. Also, some immune mechanisms that are active against viruses may diminish antibacterial immune responses, and vice versa. It may be that periodontitis is the result of extensive and partly opposing immune responses against viralbacterial combined infections (179) . Major advances in the diagnosis, prevention and treatment of periodontitis probably depend upon a better understanding of the pathogenic infections and the associated host responses.

This review article presents evidence that viruses and bacteria in aggregate produce a greater pathogenic effect than the sum of the individual agents, and discusses how the concept of a herpesviral-bacterial combined infection may change our understanding of the pathogenesis, and possibly the management, of destructive periodontal disease. Emphasis is placed on synergistic interactions between periodontal Epstein-Barr virus and cytomegalovirus, and major suspected periodontopathic bacteria.

Respiratory disease represents the best-known example of serious viral-bacterial co-infections (113, 129) . Worldwide, respiratory infections give rise to more disease and death than any other human infection and are one of the leading causes of morbidity and mortality (121) . Seasonal influenza in the USA is estimated to result in more than 200,000 hospitalizations and 36,000 deaths annually (218) . The great ÔSpanish fluÕ influenza pandemic of 1918-1919 caused 20-50 million deaths worldwide and an estimated 675,000 deaths in the USA, affecting mostly young adults and not the usual children and old individuals. At the peak, half of the worldÕs population was clinically infected. During the influenza pandemic years of 1918-1919 (type A influenza, subtype H1N1) and 1957-1958 (type A influenza, subtype H2N2), the incidence of secondary bacterial pneumonia, the most common cause of excess mortality during influenza outbreaks, varied between 2 and 18% in different populations (123) . The influenza A (swine flu) pandemic of 2009 involved a novel H1N1 strain. The main bacteria in influenza-associated pneumonia are Streptococcus pneumoniae, alphahemolytic streptococci, Haemophilus influenzae, Staphylococcus aureus and Moraxella (Branhamella) catarrhalis (123) . A combined infection with influenza viruses and S. aureus causes particularly severe and fatal pneumonia in both children and adults (29) . Respiratory viruses other than the influenza viruses can also interact pathogenically with bacterial pathogens, for example human parainfluenza virus with S. pneumoniae (50) and adenovirus with Bordetella pertussis (185) . A study of community-acquired childhood pneumonia revealed that mixed viralbacterial infections, which comprised about 75% of the pneumonias studied, resulted in more severe inflammation and clinical illness than single viral or bacterial infections (87) .

Viruses can induce alterations in the respiratory tract, which allow resident bacteria to multiply to levels capable of inducing inflammation (6) . Respiratory tract viruses can inhibit the ciliary activity of the respiratory epithelium and thereby increase the risk for bacterial superinfection (6) . Respiratory cells infected with influenza A virus, respiratory syncytial viruses or adenoviruses show enhanced bacterial adherence in both in vitro and in vivo model systems (69) . The influenza virus may predispose to secondary bacterial pneumonia by denudating the respiratory epithelium and exposing basement membrane elements (e.g. fibrinogen) to which bacteria can attach (47, 123 ). An influenza virus infection can also cause prolonged desensitization of lung sentinel cells to toll-like receptor ligands, resulting in a reduced presence of neutrophils and an increased secondary bacterial load (39) . In addition, alteration of neutrophil functions by the influenza virus may decrease the clearance of pulmonary bacteria (97, 104) .

Acute otitis media has been linked to synergistic interactions between viruses and bacteria (7) . Otitis media is often associated with viruses, such as rhinovirus, respiratory syncytial virus, adenovirus, coronavirus, influenza virus (232) , cytomegalovirus (238) and other herpesviruses (20) , as well as with bacteria, including H. influenzae, S. pneumoniae, M. catarrhalis, and Prevotella and Peptostreptococcus species (19) . Acute otitis media with a tympanostomy tube showed S. pneumoniae, S. aureus, H. influenzae, Pseudomonas aeruginosa and yeast (166) . Viruses were detected in 65% of acute otitis media samples that were positive for H. influenzae, in 77% of samples that were positive for S. pneumoniae and in 73% of samples that were positive for M. catarrhalis (170) . Tympanic membrane changes with acute otitis media are more severe in patients co-infected with respiratory viruses and bacteria than in patients who have a single infection with either of the two types of infectious agents (237) . Viruses interact with bacteria in acute otitis media to boost the local inflammatory process and also have a profound adverse effect on resolution of the disease (75) .

Other human diseases seem also to have a combined viral-bacterial etiopathogeny. Recurrent bacterial sinusitis may develop as a complication to viral colds (5) . Infectious mononucleosis caused by infection with Epstein-Barr virus can give rise to tonsillar upgrowth of Prevotella intermedia and Fusobacterium nucleatum, and subsequently pharyngotonsillitis (18) . The LemierreÕs syndrome, which is characterized by severe pharyngitis and sepsis, can occur as a sequel to Epstein-Barr virus-induced mononucleosis and is frequently associated with an overgrowth of Fusobacterium necrophorum (53) . Gastroenteritis is often associated with mixed infections of viruses (rotavirus, adenovirus, norovirus, astrovirus) and bacteria (pathogenic Escherichia coli, Salmonella, Shigella, Campylobacter jejuni), and the combined viral-bacterial infection can lead to enhanced pathosis (117) . Appendicitis has been associated with measles virus, adenovirus and herpesviruses, as well as with Bacteroides fragilis, E. coli, Yersinia, Salmonella and Shigella (91). In Helicobacter pylori-associated gastritis, the activation of a latent Epstein-Barr virus infection by monochloramine from infiltrating neutrophils can aggravate the gastric disease (128) . In organ transplant recipients, cytomegalovirus and human herpesviruses type 6 or type 7 can induce serious opportunistic infections with bacteria, fungi, protozoa and other viruses, probably as a result of immunomodulation by the herpesviruses (12, 137, 150) . A renal transplant patient developed a subhepatic abscess as a result of infection with cytomegalovirus and P. gingivalis (103) . Vascular diseases occur at a higher frequency as a result of a combined infection with herpes simplex virus and Chlamydia pneumoniae (165) or periodontopathic bacteria (224) than from a single infection with either of these agents. Rheumatic heart disease and autoimmune myocarditis may involve dual infection with coxsackievirus and group A streptococci, which have the potential to dysregulate immune mechanisms and trigger an autoimmune reaction (167) . HIV infection causes a variety of severe bacterial, fungal and viral infections (46, 100, 174, 242) . Moreover, P. gingivalis may up-regulate expression of the HIV-1 R5-specific co-receptor CCR5 in oral keratinocytes and thereby increase the risk for oral infection and dissemination of R5-tropic HIV-1 strains (58) . Also, various gram-positive and gramnegative bacteria of the periodontitis biofilm have the capacity to re-activate HIV in latently infected T cells, macrophages and dendritic cells (80) , and the vaginosis bacteria Prevotella bivia and Peptostreptococcus asaccharolyticus, but no other vaginal bacteria studied, are potential activators of HIV expression in monocytoid cells and T-lymphocytic cells (72) .

Animal models have been used to study viralbacterial interactions. Respiratory pathosis was enhanced in mice when the influenza virus or other viruses co-infected with bacteria (198) . In a mouse model of pathogenetic synergy, an influenza virus infection antecedent to an S. pneumoniae challenge caused pneumonia and led to 100% mortality, whereas an infection with S. pneumoniae prior to an influenza virus infection provided protection from influenza and improved survival rates (123) . Immune mediators, including cytokines and chemokines, through toll-like receptors ⁄ mitogen-activated protein kinase pathways, seem to play important roles in the pathogenesis of the influenza virus-S. pneumoniae co-infection (184) . Calves dually infected with bovine respiratory syncytial virus and Haemophilus somnus, both bovine respiratory pathogens, contracted lung disease and harbored H. somnus in the lungs, whereas the lungs of monoinfected calves showed neither pathosis nor H. somnus infection (57) . Acute otitis media was induced in 63% of mice that had been stably colonized by S. pneumoniae and then infected with the influenza virus, whereas all mockinfected mice remained free of disease (122) . Mice infected with herpes simplex virus, adenovirus or vaccinia virus revealed enhanced susceptibility to E. coli pyelonephritis (59) . Mice inoculated intraperitoneally with murine cytomegalovirus together with P. aeruginosa, S. aureus or Candida albicans exhibited mortality rates of 80-100%, whereas immunization against murine cytomegalovirus abrogated the synergistic effect on mortality for all combinations of the infectious agents studied (70) . In immunocompromised rats, a symptomatic infection with rat cytomegalovirus induced severe neutropenia and subsequently a high rate of enteric gramnegative rod bacteremia (201) . A markedly higher mortality rate was found in mice co-infected with murine cytomegalovirus and P. gingivalis than in mice mono-infected with either cytomegalovirus or P. gingivalis (203) . A significantly lower level of systemic interferon-gamma was detected in the dually infected mice than in the mono-infected mice, suggesting that P. gingivalis was able to reduce the antiviral interferon-gamma response and thus increase the pathogenicity of the co-infecting cytomegalovirus (203) .

For completeness, a latent herpesvirus infection may, in some experimental settings, up-regulate the activation state of the innate immunity, thereby providing immune benefits for the host. Mice latently infected with murine Epstein-Barr virus or cytomegalovirus can show resistance to infection with the bacterial pathogens Listeria monocytogenes and Yersinia pestis (10) . However, the herpesvirusinduced protection against bacterial infection seems to be transient, lasting only a few months (236) .

Collectively, numerous studies of natural diseases and experimental infections conclude that viralbacterial co-infections produce more disease than single infections with either of the two types of infectious agents. Early studies on viral-bacterial synergism emphasized a decreased clearance or an enhanced pathogenicity of the bacteria involved in the infectious complex, but more recent investigations also point to the possibility of an increased virulence of the virus. As millions of herpesviruses and bacteria can inhabit individual periodontitis sites (179) , it is reasonable to assume that herpesviralbacterial interactions also play a role in the development of human periodontitis.

Studies from various countries have found genomic copies of Epstein-Barr virus and cytomegalovirus to be present in most periodontitis lesions and to be less prevalent in gingivitis and healthy periodontal sites (Table 1) . Study-to-study variation in the detection of periodontal herpesviruses may be a result of (i) the clinical status of the study subjects, (ii) the viral diagnostic methods utilized, or (iii) true geographical ⁄ ethnic differences in herpesviral occurrence.

Identification of periodontal herpesviruses is influenced by the sample site and method utilized. Because pathogen loads typically peak during periods of active disease, herpesvirus recovery from periodontal sites depends greatly on the accuracy of the clinical diagnosis. The difficulty of defining periodontitis for the purpose of research was recently pointed out (156) . The use of patient age as a main criterion for distinguishing between ÔaggressiveÕ and ÔchronicÕ periodontitis may not be a reliable indicator of active or stable disease. The term ÔnormalÕ periodontal site is sometimes used to suggest absence of attachment loss rather than inflammation-free gingiva. As herpesviruses may enter even slightly inflamed periodontal sites, sensitive polymerase chain reaction (PCR) techniques may show viruses in ÔunhealthyÕ control sites. Conversely, periodontal therapy can markedly reduce herpesvirus subgingival counts and thus cause a significant underestimation of the viral load associated with untreated disease (177) . Reliable data on the prevalence and quantity of periodontal herpesviruses may require study of virgin lesions with a well-defined disease-activity status.

Studies that examine a small number of periodontal sites in each subject will inevitably underestimate the prevalence of herpesviruses in the study population and the total number of herpesvirus genome-copies in the entire periodontium. Studies of herpesviruses in subgingival sites will also not account for the substantial herpesvirus counts residing within inflamed gingival tissue (98, 168) .

The era of relying upon in vitro cell culture for routine laboratory diagnosis of viral infections has truly passed. Viral isolation indicates an active and possibly disease-producing infection, but isolation of viruses is difficult, costly and time-consuming. High-fidelity PCR-based techniques have become the standard for identification and quantification of periodontal herpesviruses (99, 146) , and the transcription of late herpesvirus genes for structural proteins is understood to signify productive viral replication and is commonly used to indicate an active herpesvirus infection (60, 124) . PCR identification of oral herpes simplex virus may yield two-to fourfold more positive samples than viral culture (42, 227) . Nested PCR may unveil more periodontal sites that are positive for cytomegalovirus than viral culture or real-time PCR (16) or end-point detection PCR (23) . Nested PCR technology is particularly efficient in detecting low viral loads (168) . However, PCR primers that amplify templates of microbial communities at different efficiencies may yield biased results (86) . Misamplification and falsepositive herpesvirus results may emerge when there are shared regions of nucleotide sequences between herpesvirus species and unknown infectious agents. Nevertheless, as periodontal Epstein-Barr virus and cytomegalovirus have been identified using a variety of PCR primers in platforms of end-point detection PCR, nested PCR, reverse transcription PCR and realtime PCR, the risk of a systematic misidentification of these viruses is small. However, different PCR primers and protocols may detect herpesviruses with a varying degree of proficiency (28) , and technical expertise and quality assurance methods vary among laboratories (102, 144, 183) . Preparation of the target nucleic acid constitutes a particularly vulnerable stage of PCR protocols.

The genotype distribution and seroprevalence of Epstein-Barr virus (82, 175) and cytomegalovirus (147, 168) differ among populations. Similarly to medical infections (157) , some herpesvirus subtypes may exhibit increased periodontopathogenicity. Glycoprotein B (gB) homologues within the herpesvirus family participate in virus entry and cell-to-cell spread, and the encoding gene is commonly used in genotyping (145) . The cytomegalovirus gB-II genotype seems to predominate in chronic periodontitis, whereas the cytomegalovirus gB-I genotype is more closely related to gingivitis and a healthy periodontium (235) . In addition, herpesviruses occur at a higher prevalence in low-income countries than in affluent countries (3) , and the herpesvirus seroprevalence in high-income countries exhibits significant racial, educational and socioeconomic disparities, starting at an early age and persisting into middle age (43, 44, 243) .

Epstein-Barr virus and cytomegalovirus genomes have been identified in periodontitis lesions with prevalences ranging from a few per cent to more than 80% (Table 1) . Cytomegalovirus seropositivity was identified in 95% of patients with periodontitis but in only 74% of patients with gingivitis (P = 0.057) (88) . Epstein-Barr virus (105) and cytomegalovirus (240) are also common inhabitants of peri-apical lesions of endodontic origin. Cytomegalovirus was detected in 32% of peri-apical abscesses exhibiting spontaneous pain (23) .

To assess, in more detail, the linkage between herpesviruses and periodontitis, patients have been grouped according to disease severity. Table 1 reveals the presence of Epstein-Barr virus in 65% of aggressive periodontitis lesions and in 49% of chronic periodontitis lesions and of cytomegalovirus in 44% of aggressive periodontitis lesions and in 44% of chronic periodontitis lesions. Although the prevalence of Epstein-Barr virus and cytomegalovirus was similar in aggressive and chronic periodontitis, the herpesvirus infection may differ qualitatively in the two diseases. An active (ÔproductiveÕ) cytomegalovirus infection tends to be associated with periodontal sites showing no radiographic evidence of alveolar crestal lamina dura (211, 219), a finding consistent with disease-active periodontitis (158) . An active cytomegalovirus infection has also been linked to increased gingival inflammation in immunosuppressed organ-transplant recipients (141) . An acute herpes simplex virus-type 1 infection in a 26year-old patient caused, within a few hours, extensive gingival recession (155) . By contrast, a latent (Ônon-productiveÕ) herpesvirus infection is generally associated with chronic periodontitis sites exhibiting little propensity for disease progression (16) .

Quantitatively, significantly more herpesvirus genomic copies inhabit progressive and untreated periodontitis lesions than stable or treated periodontitis sites (89, 90, 179) . As many as 8.3 · 10 8 Epstein-Barr virus DNA copies and 4.6 · 10 5 cytomegalovirus DNA copies have been detected in samples of individual periodontal pockets (179) , and even higher viral loads may reside within the gingival tissue of periodontitis lesions (98). Sunde et al. (208) identified up to one million genome-copies of Epstein-Barr virus in periodontal sites of a 63 year-old patient with refractory periodontitis. Herpesviruses other than Epstein-Barr virus and cytomegalovirus (110, 118, 138, 152, 168) , as well as papillomavirus and other viruses (189) , can also inhabit periodontitis lesions. Indeed, the total count of viruses approaches that of bacteria in some advanced periodontitis lesions. The abundance of herpesvirus genomic copies in individual periodontitis lesions translates into a heavy viral load for the entire periodontium of patients with severe and widespread periodontal disease. The high number of herpesviral copies in progressive periodontitis has implications for our understanding of the etiology of the disease. Table 2 summarizes statistical relationships between periodontal herpesviruses and bacteria. A study in China found that 17% of Epstein-Barr virus-positive periodontitis lesions, and 54% of cytomegalovirus-positive periodontitis lesions, contained as many as six to eight species of major periodontopathic bacteria (40) . Periodontal Epstein-Barr virus and cytomegalovirus seem to be most closely associated with P. gingivalis and T. forsythia (179) , two bacteria with high periodontopathic potential (78) . The link between cytomegalovirus and P. gingivalis appears to be particularly strong (193) . Cytomegalovirus and P. gingivalis were linked to localized aggressive (juvenile) periodontitis in Jamaica with odds ratios of 4.6 and 7.8, respectively, but the cytomegalovirus-P. gingivalis dual infection was linked to the disease with an odds ratio as high as 51.4 (125) . The significantly higher odds ratio of the cytomegalovirus-P. gingivalis co-infection than of the sum of the individual pathogens is suggestive of a pathogenetic synergy between the infectious agents. A study, in the USA, of 140 adults with gingivitis or periodontitis, linked Epstein-Barr virus-1 and cytomegalovirus to elevated occurrence of the pathogens P. gingivalis, T. forsythia, P. intermedia, Prevotella nigrescens and Treponema denticola ( Table 3 ). In a study from Japan, P. gingivalis comprised 0.25% of total salivary bacterial counts in Epstein-Barr virus-positive periodontitis patients but only 0.02% (a 13-fold difference) in Epstein-Barr virus-negative patients (205) . Periodontal Epstein-Barr virus (179, 207) and cytomegalovirus (85, 125, 140, 219) have also been related to the subgingival presence of the major periodontopathogen Aggregatibacter actinomycetemcomitans. An association between herpesviruses and anaerobic bacteria has also been demonstrated in periapical pathosis (171) .

Herpes simplex virus may participate in periodontal disease in a subgroup of individuals (85, 89, 110, 138, 176, 178) . Herpes simplex virus-1, in combination with P. gingivalis, T. forsythia, P. intermedia or A. actinomycetemcomitans, has been linked to periodontitis (85) , and, in combination with T. denticola, T. forsythia or Dialister pneumosintes, to periodontitis and necrotic dental pulp (138) .

Despite a large body of supporting evidence, welldesigned studies of diverse populations are still needed to corroborate the findings of a relationship between various herpesviral-bacterial consortia and periodontal disease severity. Research is also needed to determine the extent to which herpesviral-bacterial co-infections in periodontitis represent pathogenetically significant interactions or merely an independent etiologic importance of each of the infectious agents.

The periodontal immune response to herpesviral and bacterial infections is bifunctional with both tissue- 

Slots et al. (196) Campylobacter rectus Saygun et al. (179) Treponema denticola Contreras et al. (31) protective and tissue-destructive effects, depending upon the type of the infectious agent and the immune competency of the individual. Features of herpesviral and bacterial infections of importance in the etiopathogeny of periodontitis are highlighted below.

Infectious agents causing periodontitis must be able to colonize periodontal sites, overcome local host defenses, proliferate in periodontal sites and participate in the breakdown of periodontal tissues (192) . Also, periodontitis develops in a multistep process that reflects the dynamic interplay between the periodontal infectious agents and the host immune responses.

Successful immune control of a periodontal infection depends on a highly coordinated series of host defenses (36) . The host identifies pathogens as nonself by recognizing pathogen-associated molecular patterns (116) . Pathogen recognition receptors and signaling pathways subsequently activate cells of the immune system. Cytokines mediate the interaction and regulation of immune cells. Optimally, the host executes immune responses sufficient to control the pathogens, but also ensures suppression of excessive immune reactions in order to limit the pathological consequences of inflammation. If uncoordinated, the host immune response by itself may cause pathosis.

Immune regulation is mediated by activated CD4 T cells, which, on the basis of characteristic cytokine profiles, are grouped into T helper (Th) type 1 (Th1), Th2, Th17 and induced T regulatory (iTreg) cells (52) . Activated T helper cells secrete either pro-or antiinflammatory cytokines depending on the type and the mode of the immunologic stimulus. Th1 cells drive the type-1 pathway (Ôcellular immunityÕ) to fight enveloped viruses and intracellular bacterial pathogens, eliminate cancerous cells and stimulate delayed-type hypersensitivity reactions (93) . Activated Th1 cells are characterized as pro-inflammatory and are a source of interferon-gamma, interleukin (IL)-1, IL-2, IL-6, IL-12, IL-18, IL-23 and tumor necrosis factor-alpha. Cytomegalovirus and other herpesviruses induce Th1-type pro-inflammatory cytokine responses (163) . Pro-inflammatory cytokines ⁄ chemokines promote leukocyte infiltration into the site of infection and play a crucial role in orchestrating the immune response against herpesvirus infections by enhancing the proliferation of T lymphocytes and facilitating cell-cell signaling.

Th2 cells induce the type-2 pathway (Ôhumoral immunityÕ), which is linked to differentiation of B cells and antibody production (93) . Antibodies are of critical importance in the defense against nonenveloped viruses and extracellular bacterial pathogens. The Th2 response evokes the secretion of the antiinflammatory and immunoregulatory cytokine IL-10, and of IL-4, IL-5, IL-6, IL-9 and IL-13. However, the Th1 ⁄ Th2 concept has inconsistencies, and several human and animal cytokine activities fail to fall into exclusive Th1 or Th2 patterns (93) . IL-6 can act as both a pro-inflammatory and an antiinflammatory cytokine. Also, the various cytokines in inflammation have both unique and redundant roles, and can augment or inhibit the effect of other cytokines, complicating the assessment of the biological significance of individual cytokines. In addition, Th1 cells can inhibit Th2 cell differentiation by their expression of interferon-gamma and IL-12, while Th2 cells can inhibit Th1 cells by their expression of IL-4, IL-10 and transforming growth factorbeta (233) . The balance between Th1 and Th2 immune responses is regulated by iTreg cells and influenced by genetic and environmental factors. The feedback mechanisms among Th1 and Th2 type cytokines aim at amplifying or dampening local inflammatory responses in order to sustain an adequate host response and to safeguard the host against bursts of exaggerated inflammation.

Th1 and Th2 cell responses have received considerable interest in periodontal research (55, 71) . As Th1 and Th2 immune responses are partly antagonistic to each other, the net effect of the combined Th1 ⁄ Th2 immune response may determine whether a periodontal infection leads to limited gingival tissue reaction or to breakdown of periodontal attachment. Pro-inflammatory cytokines occur at elevated levels in severe periodontitis lesions (62), where they exert immunomodulating activity as well as participate in collagen degradation and bone resorption (92) . As periodontitis lesions concurrently harbor herpesviruses (which trigger Th1-based cellular immunity) and pathogenic bacteria (which induce Th2-based humoral immunity), the diseased periodontium may experience a changing dominance of either herpesviral or bacterial immunity.

Some studies have suggested that Th1 cells are associated with stable periodontitis and that Th2 cells are associated with disease progression (56, 186) . Other studies have reported a predominance of Th1 cells, or a reduced Th2 response, in diseased periodontal tissue (55, 56, 216) . These findings are not necessarily in conflict with each other. A predominance of Th1 cells in disease-stable sites may suggest a successful anti-herpesvirus defense, and elevated levels of Th1 cells in progressing lesions may reflect an ongoing effort by the host to control an active herpesvirus infection. Similarly, the dis-pute about a dominance of either T cells or B cells in periodontitis sites may be partly unfounded (11, 71) . A preponderance of T cells may reflect the attempt of the host to control an active herpesvirus infection. High B-cell counts may be caused by specific antibody production or the result of an active Epstein-Barr virus infection, which, in the absence of sufficient cytotoxic T-cell function, can induce B-cell proliferation by polyclonal stimulation (21) .

The eight human members of the herpesvirus family infect parenchymal cells, connective tissue cells, epithelial cells, various hematopoietic cells and other cell types, and can cause a variety of illnesses by mechanisms that are direct, indirect or immunoregulatory (151, 189, 190) . The clinical outcome of herpesvirus infections ranges from subclinical or mild disease to encephalitis, pneumonia and other potentially lethal infections, and even to cancer, including lymphoma, sarcoma and carcinoma (190) . The great majority of adults are carriers of Epstein-Barr virus and cytomegalovirus. Once infected, a person harbors the herpesvirus for life.

The herpesvirus replication-cycle includes binding of viral envelope glycoproteins to cell-membrane receptors, internalization and dismantling of the virus particle, migration of the viral DNA to the cell nucleus, transcription of viral genes, assembly of the virion and viral egress from the infected cell (Fig. 1) . Herpesviruses destroy infected cells by active lytic replication. After primary infection, herpesviruses remain latent with limited expression of viral genes, albeit retaining the transcriptional and replicational capacity. Latency ⁄ persistence is maintained for Epstein-Barr virus in resting memory B lymphocytes, and for human cytomegalovirus in dendritic cells and in monocytes and their progenitors.

Active herpesvirus infections evoke strong innate and adaptive immune responses, which include both immune activation and immune suppression (34, 131, 164) . Key effector cells of the innate immune system are dendritic cells, monocytes ⁄ macrophages and natural killer cells, whose function is to limit the viral burden until cells of the adaptive immunity become available to suppress the infection. The effector cells recognize viral proteins via toll-like receptors, natural killer cell receptors or other pattern recognition receptors. Herpesvirus DNA reacts with toll-like receptor 9, which is significantly up-regulated in periodontitis lesions compared with gingivitis lesions (88) . Cells of innate immunity employ cytokine secretion and cell-mediated cytotoxicity as the primary anti-herpesvirus effector mechanisms. Macrophages and polymorphonuclear leukocytes can destroy antibody-coated virions or virus-infected cells via reactive oxygen species, nitric oxide and activated caspases. Natural killer cells are an important source of interferon-gamma and are able to kill herpesvirus-infected cells via virus-specific antibodydependent cell-mediated cytotoxicity or via antibody-independent mechanisms. In addition, natural killer cells share similarities with cytotoxic T cells and may play a role in the adaptive immunity (206) .

The phagocytic cells internalize, process and express herpesvirus-derived immunogenic peptides on their surface which, after linkage to major histocompatibility complex (MHC) molecules, can attach to and activate Th1 cells of the adaptive immune system. Cytomegalovirus can have a profound influ-ence on the immune system of healthy individuals (25) , and cytomegalovirus reactivity has been detected in as many as 30-50% (up to 80%) of the T cells of cytomegalovirus-seropositive elderly individuals (200) . Recognition of the herpesvirus proteins causes the induction of the pro-inflammatory transcription factor nuclear factor-kappaB (NF-jB) with a subsequent release of cytokines and mobilization of CD8 + T cells (116) . Activated CD8 + T cells differentiate into virus-specific cytotoxic T cells capable of recognizing and killing cells carrying viral peptides. The release of CD8 + T-cell-associated antiviral cytokines, such as interferon-gamma and tumor necrosis factor-alpha, may also inhibit viral replication without killing the infected cell (66) .

Herpesvirus infections in immunocompetent individuals also induce antibody production against herpesvirus proteins (209) , and patients with periodontitis exhibit an elevated level of antibodies against herpesviruses (77, 88) . However, antibodies against herpesviruses and against many other enveloped viruses do not ensure a favorable clinical outcome. Herpesviruses have developed strategies to downregulate antiviral host defenses in order to persist in the midst of intense inflammation (154) . Immunosuppression may thus take place, even in the presence of a substantial immune activation. Herpesviruses encode immunoevasive glycoproteins that interfere with antigen presentation, T-cell immune surveillance and natural killer cell function (130) . The viral proteins seek to alter or mimic MHC protein function, leukocyte activation and migration, induction and activity of cytokines and interferons, antibody-based defense mechanisms, and host cell susceptibility to apoptosis (programmed cell death), which is the principal mode of physiologic elimination of cells (130) .

Despite elaborate immune-evasion efforts by herpesviruses, the host immune system generally prevails in immunocompetent subjects, perhaps because of the host immune-sensing mechanisms that terminate viral gene expression before the assembly of infectious virions (161) . However, herpesvirus disease incidence is elevated in immunologically immature subjects, and in individuals who are immunosuppressed as a result of advancing age (immunosenescence), diseases [e.g. HIV ⁄ acquired immune-deficiency syndrome (AIDS)] or treatments (e.g. cancer chemotherapy, radiotherapy, pharmacological immunosuppression with organ transplantation, high-dose corticosteroids).

Bacterial infections evoke functionalities of both the innate immune system and the adaptive immune system. Bacterial species attach to specific toll-like receptors to establish some degree of specificity in the innate immune system and subsequently in the adaptive immune system (76, 94) . Bacterial pathogens detected by toll-like receptors on the surface of macrophages activate NF-jB-mediated transcription of cytokines and chemokines (136) . Macrophages release cytokines and chemokines to recruit neutrophils in the innate immune system and serve as antigen-presenting cells for lymphocytes in the adaptive immune system.

Neutrophils comprise more than 90% of the inflammatory cells in periodontal pockets (45) . Their importance in periodontal defense is evidenced by the observation of severe periodontitis in most subjects exhibiting major neutrophil deficiencies (38) . Neutrophils phagocytize and kill ingested bacteria by means of reactive oxygen species (e.g. the myeloperoxidase system and hypochlorite), antimicrobial proteins (e.g. defensins, lysozyme and lactoferrin), degradative enzymes (e.g. elastase and cathepsin G) and other microbicidal pathways (38) .

Extracellular pathogenic bacteria activate Th2 cells, which release anti-inflammatory cytokines and commit B cells to antibody production. P. gingivalis seems to evoke primarily a Th2-type response in periodontitis patients (79) . Antibacterial antibodies of the IgG1 subclass play important roles in opsonization and complement activation (38) . Lipopolysaccharide of P. gingivalis and of other periodontopathic bacteria can also activate complement via the alternative pathway and induce the release of pro-inflammatory cytokines (41) . Complement assists antibodies by acting as an opsonin, by lysing bacterial cells and by attracting lymphocytes and neutrophils to the site of infection.

Intracellular bacteria trigger a Th1-mediated immune response, which includes the release of interferon-gamma, pro-inflammatory cytokines and the IgG2 antibody isotype (48, 220) . IgG2 serum antibody occurs at high levels in patients with localized aggressive (juvenile) periodontitis and, despite being a less efficient opsonin than IgG1 and IgG3, seems to protect against further tissue destruction (181). P. gingivalis (101, 106), A. actinomycetemcomitans (26, 169) and other periodontal species (27, 222, 225) have the ability to invade cells of the periodontium and thus may trigger a Th1, as well as a Th2, immune response. P. gingivalis-specific T cells can produce both Th1 and Th2 cytokines irrespectively of the type of antigen-presenting cells (54) .

Epstein-Barr virus-cytomegalovirus co-infection tends to be associated with severe types of oral disease, including aggressive periodontitis (89, 179, 219, 239) , chronic periodontitis (22, 235) , periodontal abscesses (180) , acute necrotizing ulcerative gingivitis (30) , symptomatic peri-apical lesions (194) and oral ulcers (210) . Cytomegalovirus-herpes simplex virus co-infection has also been associated with increased gingival inflammation and periodontal attachment loss (110) . Persons exhibiting a high rate of shedding of Epstein-Barr virus into saliva tend to reveal sali-vary cytomegalovirus DNA more often than those with a low rate of Epstein-Barr virus shedding, which may be suggestive of a cooperative relationship between the two viruses (64) . Infants and children infected with both Epstein-Barr virus and cytomegalovirus have shown stronger T-cell responses and more severe disease than children mono-infected with either of the viruses (226) . Feline calicivirus and feline herpesvirus were both shed in 88% of cats with chronic gingivostomatitis compared to 21% of cats without chronic oral inflammatory disease (112) .

A concomitant infection with two herpesviruses may activate latent viral genomes by the mechanism of reciprocal transactivation, which connotes that gene products of one virus trigger the transcription of another virus (231) . Two active viruses, each providing their own unique set of virulence factors, can result in a particularly severe disruption of the immune system and accelerate a disease process (213) . Herpesvirus reactivation causes a major spike in cytotoxic T cells and pro-inflammatory cytokines (132) , but also produces virus-derived homologues of human IL-10 (108) and other inhibitors of the antiviral Th1 cell-mediated defense (195) . Cytomegalovirus IL-10 suppresses NF-jB activation and subsequently transcription of tumor necrosis factor-alpha and IL-1beta (135) . Specific genotypes of IL-10 are correlated with an increased risk of progressive periodontitis (35) . Other mechanisms of interaction between two viruses include the enhanced expression of viral receptors and co-receptors in target cells as well as the production of superantigens (120) .

Herpesviruses and specific bacteria in aggregate are also associated with enhanced severity of periodontal disease (179) . Herpesviruses may aid in the initial colonization and upgrowth of periodontopathic bacteria. An active human cytomegalovirus infection of primary periodontal pocket epithelial cells or HeLa cells enhances the adherence of A. actinomycetemcomitans in a dose-dependent mode (217) ; viral infections may destroy epithelial cells and expose new bacterial attachment sites in the basement membrane; and herpesvirus glycoproteins expressed on the surface of infected host cells may serve as receptors for bacteria. In addition, as A. actinomycetemcomitans can bind to the Fc part of the IgG molecule (221), the organism may attach to antibodies directed against the herpesvirus-derived glycoproteins. Consistent with the concept of herpesviral-bacterial synergism, the initial phases of localized aggressive (juvenile) periodontitis demonstrate an active cytomegalovirus infection (219) and a remarkable increase in A. actinomycetemcomitans subgingival counts (197) . Cytomegalovirus has, in several animal models, shown the ability to impair neutrophil chemotaxis, phagocytosis, oxidative burst and intracellular killing capacity (2); some of these neutrophil defects have also been described in localized aggressive periodontitis (182) . Individuals who show an absence of herpesviral infection or of herpesviral re-activation may exhibit a normal periodontium or minimal or nonprogressive disease, even in the presence of periodontopathic bacteria.

Herpesviruses can compromise the immune control of periodontopathic bacteria by a variety of mechanisms. Polymorphonuclear leukocytes seem to exhibit less efficient chemotaxis and bactericidal capacity in periodontitis patients with subgingival herpesviruses than in herpesvirus-free subjects (142) . Anti-herpesvirus cytotoxic ⁄ suppressor T cells, which are present at elevated levels in aggressive periodontitis lesions (187) , may adversely affect mammalian cells involved in the antibacterial periodontal defense. Th1 cytokines associated with active herpesvirus infections reduce Th2-cell responses and thereby the antibody-mediated control of pathogenic bacteria. Herpesviruses are capable of subverting macrophage (51, 63, 109) , complement (111) and neutrophil (1) functions of importance in the antibacterial host defense. P. gingivalis and other exogenous-like species, which may be primarily controlled by antibody-mediated mechanisms (159, 215) , may benefit most from a reduction in antibacterial immunity and outgrow co-existing indigenous microorganisms. Taken together, an active herpesvirus infection has the potential to impair antibacterial host defenses and may trigger a microbial shift towards a more virulent subgingival microbiota.

The lipopolysaccharide of gram-negative periodontopathic bacteria, most prominently P. gingivalis, can induce a Th1-cell type release of pro-inflammatory cytokines (41, 223) , and may co-operate with cytomegalovirus in stimulating IL-1beta gene transcription (229) . As Th1 cell activities can inhibit Th2 antibacterial immunity, the lipopolysaccharideinduced Th1 cell response may partly constitute an immune-evasive maneuver by periodontopathic bacteria in their efforts to survive in a hostile environment. On the other hand, the Th1 immune response may help to control P. gingivalis infections when they occur intracellularly (101, 106) . Also, as herpesviruses may induce the overgrowth of P. gingivalis and other pathogenic species (179, 193, 196) , the Th1-mediated anti-herpesvirus immune response may indirectly aid in containing the population of periodontopathic bacteria.

It is equally conceivable that a bacterial infection can pave the way for a herpesvirus clinical infection. P. gingivalis sonicate has the potential to re-activate Epstein-Barr virus (205) . The etiopathogenic model for periodontitis, detailed below, proposes that a bacterial-viral-bacterial sequential infection gives rise to the disease. Dental biofilm bacteria initiate gingivitis with an influx of herpesvirus-infected monocyte ⁄ macrophages, T cells or B cells (32) . A subsequent immunosuppressive event may then re-activate latent herpesviruses, resulting in the release of pro-inflammatory cytokines and matrix metalloproteinases, and in the overgrowth of periodontopathic bacteria.

Proteolytic enzymes of periodontal bacteria can degrade pro-inflammatory cytokines and other hostdefense systems (83, 212) , thereby potentially activating a co-existing latent herpesvirus infection. Human gingival epithelial cells challenged with P. gingivalis showed a fourfold increase in the primary cytokine IL-1beta level, but the related secondary cytokines IL-6 or IL-8 were virtually undetectable as a result of direct degradation by P. gingivalis proteases, with lysine gingipain being the most effective (202) . Thus, in addition to subverting host defenses against bacterial infections (153) , gingipains may exert periodontopathogenicity by perturbing cytokine-mediated antiviral host responses.

Lastly, herpesviruses and bacteria may interact bidirectionally, as shown in a study of infected wound chambers in mice (203) , adding another level of complexity to the analysis of the pathophysiology of periodontitis. Mice co-infected with murine cytomegalovirus and P. gingivalis in wound chambers exhibited significantly higher mortality than mice infected with either murine cytomegalovirus or P. gingivalis, or simultaneously with murine cytomegalovirus and E. coli (203) . The increase in cytomegalovirus pathogenicity was related to an ability of P. gingivalis to suppress interferon-gamma, perhaps because of the proteolytic activity of the organism. A low level of interferon-gamma was found despite the capacity of cytomegalovirus to trigger the induction of interferon-gamma, and despite the ability of bacterial lipopolysaccharide, via cytokine simulation, to induce interferon-producing CD8 + T cells (160) . Adding further to the complexity, cytomegalovirus encodes genes capable of inhibiting the interferon response and antiviral processes (37) .

In teleological terms, periodontitis can perhaps be viewed as a by-product of the efforts by the host to control periodontal herpesvirus infections and avoid systemic viral diseases. Periodontal pro-inflammatory cytokines, in spite of being capable of inducing collagen degradation and alveolar bone resorption (92) , may actually be overall beneficial by helping to prevent activation and systemic dissemination of virulent viruses (65) . Similarly, although CD8 + T cells may impede antibacterial defenses, they also confer a critical antiviral function. Periodontitis may represent an excellent example of how host immune responses can exert simultaneously protective and destructive functionalities.

The finding of abundant herpesviruses in periodontitis lesions redefines the pathogenic paradigm of the disease. Fig. 2 describes a model for the development of periodontitis, which as its core has a sequential infectious process that proceeds from bacteria to herpesvirus to bacteria (191) . Initially, bacteria in the dental biofilm induce gingivitis, which permits latent herpesviruses, embedded in the DNA of macrophages, T lymphocytes and B lymphocytes, to infiltrate the periodontium (32) . Cytomegalovirus can replicate in gingival tissue (68) , which may help to sustain the periodontal infection. Re-activation of the latent herpesviruses may occur spontaneously or during periods of decreased host defense, resulting from drug-induced immunosuppression, concurrent infection, unusual and prolonged emotional stress, hormonal changes, physical trauma, etc. Probably not coincidentally, most herpesvirus-activating factors are also suspected risk factors ⁄ indicators for periodontitis (162) .

In response to the active herpesvirus infection, the host elicits a robust T-cell-mediated immune response, comprised primarily of CD8 + T cells. To counteract the hostile host environment, herpesviruses in turn execute strategies to down-regulate antiviral host defenses. Herpesviruses evade immune responses by disintegrating components of the MHC and interfering with antigen presentation, by silencing natural killer cells, by expressing a viral homolog of IL-10, by diverting potent cytokine responses and by inhibiting apoptosis (189) . The encounter between antiviral host defenses and virally mediated anti-host responses results in a major release of pro-inflammatory cytokines that have the potential to activate osteoclasts (13, 71) and to impair antibody-mediated host defenses against exogenous-like bacterial spe-cies, such as P. gingivalis and A. actinomycetemcomitans (179) . The ensuing increase in pathogenic bacteria provides additional mechanisms of periodontal tissue destruction (78) .

Cytomegalovirus or other herpesviruses can exert acute cytopathogenic effects on fibroblasts, epithelial cells, keratinocytes, endothelial cells, inflammatory cells and bone cells (17) . An active herpesviral infection in severely immunocompromised patients may directly destroy periodontal cells and tissue by cytotoxic mechanisms, as seen in patients with necrotizing ulcerative gingivitis and noma. Herpesvirus infections may participate in oral collagen degradation, as suggested in in vivo (172) and in vitro (14) studies, and potentially interfere with periodontal tissue turnover and healing. Herpesvirus infections have also been related to diminished repair in periodontal guided tissue regeneration (199) and to dry socket formation after tooth extraction (73, 74) .

In the herpesviral-bacterial model of periodontitis, herpesvirus-related cytopathogenic effects, immune evasion, immunopathogenicity, latency, re-activation from latency and tissue ⁄ site tropism comprise important aspects of periodontal pathosis. It is likely (143) . The decrease in post-treatment herpesvirus counts is probably caused by a reduction in gingivitis and thus in the numbers of virally infected inflammatory cells. Similarly, the low herpesvirus counts in healthy periodontal sites may be the result of a virtual absence of infected inflammatory cells. Cytomegalovirus was also not detected in healthy peri-implant sites (139) . Saliva can contain high genome-copy counts of herpesviruses. Medically healthy persons have been shown to contain 3.6 · 10 2 to 1.6 · 10 9 copies ⁄ ml of Epstein-Barr virus in saliva (173) and from 6 to 2.2 · 10 6 per 0.5 lg of DNA (228) . Salivary cytomegalovirus DNA was detected in one-half of periodontitis patients at levels of 3.3 · 10 3 -4.2 · 10 4 copies ⁄ ml, whereas the virus was not detected in the saliva of periodontally normal individuals or in complete denture wearers (173) , affirming the close relationship between cytomegalovirus and periodontitis. Herpesvirus-6 and herpesvirus-7 can occur in saliva with prevalences exceeding 90% and in concentrations of several million genome-copies (126) . Herpesvirus-6 was detected in the whole saliva of 68% of healthy volunteers from Brazil (152) . Herpesvirus-8 reached salivary loads of 2.6-4.1 · 10 6 genome-copies ⁄ ml in a renal allograft recipient (4). HIV-infected individuals harbor higher salivary cytomegalovirus counts than non-HIV-infected persons (114, 115) , and HIV-infected individuals receiving antiretroviral therapy demonstrate higher salivary prevalence and copy counts of Epstein-Barr virus and other herpesviruses than normal control subjects (127) . HIV-infected individuals have revealed salivary median copy counts of 5.3 · 10 5 ⁄ ml for Epstein-Barr virus and 3.3 · 10 3 ⁄ ml for cytomegalovirus (64) .

Conventional periodontal treatment has reduced salivary Epstein-Barr virus genome-copy counts by 13-fold and salivary cytomegalovirus copy counts by 65-fold (177), salivary Epstein-Barr virus counts in two children with Kostmann syndrome by more than 100fold (241) , and the average Epstein-Barr virus counts per ml of saliva from 946,000 to 9,010, and with six of 11 (54%) study patients with salivary Epstein-Barr virus pre-pretreatment not showing the virus post-treatment (81) . Several patients in the above studies exhibited gingival inflammation post-treatment; more efficacious anti-gingivitis measures may have decreased the salivary herpesvirus counts even further (188) . These treatment data suggest that diseased periodontal sites are an important source of salivary herpesviruses. The potential of periodontal therapy to decrease herpesvirus levels in saliva may reduce the risk of herpesvirus transmission and herpesvirus-related diseases among close acquaintances.

The herpesviral-bacterial model of periodontitis provides a rationale for considering new approaches to disease prevention and treatment. Sunde et al. (208) treated a patient, who exhibited refractory periodontitis and high Epstein-Barr virus subgingival copy counts, with the anti-herpesvirus drug, valacyclovir HCl, 500 mg twice a day for 10 days. The treatment suppressed subgingival Epstein-Barr virus to undetectable levels for at least 1 year and resulted in a ÔdramaticÕ clinical improvement (208) . Sunde et al. (208) proposed employing virus screening in periodontal treatment to determine when antiviral intervention is appropriate and if it has succeeded. Periodontal viruses may be identified successfully by using diagnostic DNA microarrays that are able to detect simultaneously herpes simplex virus types 1 and 2, Epstein-Barr virus, and cytomegalovirus as well as other viruses (134); or by using multiplex real-time PCR techniques to quantify simultaneously the number of genome-copies of herpes simplex virus, varicella-zoster virus, Epstein-Barr virus, cytomegalovirus and human herpesvirus-6 (49, 67) . Newer metagenomic pyrosequencing techniques may be even more proficient in identifying known and unknown periodontal viruses.

Anti-herpesvirus chemotherapy can also decrease the salivary viral load. A short course of valacyclovir, 2 g twice on the day of treatment and 1 g twice the following day, resulted in a significant decrease in the salivary occurrence of Epstein-Barr virus compared with controls (126) . Valacyclovir, 500 mg orally twice daily for 1 month, given to elite male distance runners, reduced the salivary load of Epstein-Barr virus by 82% compared with placebo (33). Valacyclovir therapy, 3 g per day for 14 days, resulted in a reduction, of more than 100-fold, of Epstein-Barr virus genome-copies in oral wash fluid of patients with acute infectious mononucleosis (9) .

Chemotherapeutics are effective against viruses in the lytic phase, but not against viruses in the latent phase, limiting their potential use to disease-active infections. Acyclovir types of drugs are acyclic nucleoside analogues that inhibit herpesviral DNA polymerase and replication of the viral genome. The orally administered acyclovir prodrug, valacyclovir, can reach serum concentrations similar to those of intravenously administered acyclovir and is prescribed for a variety of herpesviral diseases (149) . Prolonged treatment with valacyclovir at dosages of 500-1000 mg ⁄ day is well tolerated, perhaps except in immunosuppressed individuals, and the adverse events are infrequent and generally mild, with headache being reported most often (6) . To date, resistance to valacyclovir has not been clinically significant. However, randomized controlled trials are needed before anti-herpesviral chemotherapy can be considered as standard clinical practice in the treatment of advanced periodontitis.

Future management of periodontal diseases may benefit from anti-herpesviral immunotherapeutics: either prophylactic vaccines, which harness the immune system of healthy subjects to prevent infection with disease-causing viruses; or therapeutic vaccines, which stimulate the immune system into combating existing viruses and disease. An efficacious vaccine against herpesviruses may also provide clinical proof-of-principle of the periodontopathic importance of the viruses. The notion of herpesviralbacterial synergism in periodontitis implies that vaccination against herpesviruses can also contribute to the control of periodontopathic bacteria. One difficulty to overcome with prophylactic vaccination is declining immunity over time, which carries an increased risk of delayed-onset primary herpesvirus diseases with increased morbidity. The US Institute of Medicine has assigned high priority to the development of vaccines against herpes simplex virus, Epstein-Barr virus and cytomegalovirus, to be given to 12-year-old children (204) .

The etiopathogenesis of periodontitis includes virulence factors of herpesviruses and bacteria, host immune responses against viral and bacterial infections, and manipulation of host-cell processes by the infectious agents. Herpesviruses may induce periodontitis by activating specific tissue-destroying pathways of the immune system and by predisposing an individual to bacterial carriage or increased bacterial load. However, the molecular contribution of herpesviruses versus bacteria to periodontal pathosis remains little understood.

The inflamed periodontium appears to be a major site for Epstein-Barr virus and cytomegalovirus accumulation and re-activation, especially in the progressive phase of periodontal disease. A single advanced periodontitis lesion may contain up to 100 million genomic copies of herpesviruses, approaching the total viable counts of bacteria. Immunosuppressive factors are potential triggers of herpesvirus re-activation and, perhaps for that reason, are also major risk factors for periodontitis. An active herpesvirus infection in the periodontium may give rise to systemic viremia, but the magnitude and duration of this are unknown.

An active herpesvirus infection correlates with periodontitis disease activity and may be a major contributor to the periodontal immune response. Herpesviruses are potent inducers of pro-inflammatory cytokines that have the potential to activate osteoclasts and matrix metalloproteinases. An active herpesvirus infection can also impair antibacterial immune mechanisms and potentially cause an upgrowth of periodontopathic bacteria. Some periodontopathic bacteria may re-activate a latent herpesvirus infection. Synergism among herpesviruses and bacteria may play an important role in the onset and progression of periodontitis. The interplay of herpesviruses and bacteria in periodontitis may be compared to a marionette theater where the puppeteer is the virus and the puppets are the bacteria. Even if the puppeteer (virus) controls the performance (disease), both the puppeteer and the puppets are necessary for the marionette theater to function.

Obviously, it is difficult to link a herpesvirus infection that occurs in childhood to periodontitis that debuts several decades later. An important question is whether the herpesvirus-periodontitis association is etiologically based or is merely an epiphenomenon to gingival inflammation. Studies on herpesviral etiology of medical diseases face similar difficulties in delineating cause and effect. To distinguish between causality and correlation, information is needed on the extent to which an active herpesvirus infection gives rise to destructive periodontal disease, and the extent to which diseaseactive periodontitis may activate a latent herpesvirus infection. A causal relationship may be inferred if the removal of herpesviruses by specific antiviral medication arrests, reverses or prevents periodontitis. Studies assessing temporal and histological correlations may also help to elucidate the role of herpesviruses in periodontal disease. Such studies may take advantage of molecular techniques able to quantify herpesvirus loads and relate either active or latent herpesvirus infection to periodontitis-disease activity. However, the definitive answer to the periodontopathic significance of herpesviruses will probably have to await the development of efficacious antiherpesvirus vaccines.

The notion of a herpesviral-bacterial co-infection in periodontitis may turn out to be the pathogenetic Rosetta stone that unlocks many of the intricacies of the disease. Herpesvirus-induced periodontitis implies that anti-herpesvirus immunity constitutes an important aspect of achieving a long-lasting state of stable periodontal conditions. The development of herpesvirus vaccines in the not too distant future makes the topic of periodontopathic herpesviruses particularly intriguing. Control of herpesviruses by vaccination may foreshadow a future with a diminishing role for the traditional periodontal therapies of surgery and antibiotics. It is hoped that the issues raised in this review will help to steer periodontal research into new fertile fields of investigation.

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Virus-induced neutrophil dysfunction: role in the pathogenesis of bacterial infections

Seroprevalence of HHV-8, CMV, and EBV among the general population in Ghana

Extensive oral shedding of human herpesvirus 8 in a renal allograft recipient

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Microbiology of acute otitis media with tympanostomy tubes

Antigenic complementarity between coxsackie virus and streptococcus in the induction of rheumatic heart disease and autoimmune myocarditis

Human herpesvirus 7, Epstein-Barr virus and human cytomegalovirus in periodontal tissues of periodontally diseased and healthy subjects

Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythensis are components of a polymicrobial intracellular flora within human buccal cells

Microbiology of acute otitis media in children with tympanostomy tubes: prevalences of bacteria and viruses

Herpesviral-bacterial coinfection in periapical pathosis

Natural killer cells and alterations in collagen density: signs of periradicular herpesvirus infection?

Periodontitis lesions are the main source of salivary cytomegalovirus

Oral mucosal fungal infections

EBNA-1 sequence variation in Danish and Chinese EBV-associated tumours: evidence for geographical polymorphism but not for tumour-specific subtype restriction

Bacterial and viral DNA in periodontal disease: a study using multiplex PCR

Periodontitis lesions are a source of salivary cytomegalovirus and Epstein-Barr virus

Herpesviral-bacterial interrelationships in aggressive periodontitis

Quantitative analysis of association between herpesviruses and bacterial pathogens in periodontitis

Human cytomegalovirus and Epstein-Barr virus type 1 in periodontal abscesses

Cytokines and inflammatory factors regulating immunoglobulin production in aggressive periodontitis

Early-onset periodontitis: systemic aspects of etiology and pathogenesis

An international external quality assessment of nucleic acid amplification of herpes simplex virus

Immunokinetics in severe pneumonia due to influenza virus and bacteria coinfection in mice

Severe pneumonia and chronic lung disease in a young child with adenovirus and Bordetella pertussis infection

Immunopathogenesis of chronic inflammatory periodontal diseases: cellular and molecular mechanisms

Evidence for a specific crevicular lymphocyte profile in aggressive periodontitis

Selection of antimicrobial agents in periodontal therapy

Herpesviruses in periodontal diseases

Oral viral infections of adults

Herpesviruses: a unifying causative factor in periodontitis?

Black-pigmented Bacteroides species, Capnocytophaga species, and Actinobacillus actinomycetemcomitans in human periodontal disease: virulence factors in colonization, survival, and tissue destruction

The herpesvirus-Porphyromonas gingivalis-periodontitis axis

Cytomegalovirus infection in symptomatic periapical pathosis

Epstein-Barr virus in oral diseases

Cytomegalovirus periodontal presence is associated with subgingival Dialister pneumosintes and alveolar bone loss

Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in human periodontal disease: occurrence and treatment

Cooperation between viral and bacterial pathogens in causing human respiratory disease

Clinical and microbiological evaluation of a bioabsorbable and a nonresorbable barrier membrane in the treatment of periodontal intraosseous lesions

HCMV microinfections in inflammatory diseases and cancer

Prevention of bacteraemia by systemic ciprofloxacin treatment in rat cytomegalovirus-infected immunocompromised rats

The host cytokine response to Porphyromonas gingivalis is modified by gingipains

Reduced expression of gamma interferon in serum and marked lymphoid depletion induced by Porphyromonas gingivalis increase murine morbidity and mortality due to cytomegalovirus infection

Institute of Medicine (US) Committee to Study Priorities for Vaccine Development. Vaccines for the 21st century: a tool for decision making

Relationship between Porphyromonas gingivalis, Epstein-Barr virus infection and reactivation in periodontitis

Adaptive immune features of natural killer cells

Human cytomegalovirus and Epstein-Barr virus in apical and marginal periodontitis: A role in pathology

Patient with severe periodontitis and subgingival Epstein-Barr virus treated with antiviral therapy

Changes in seroprevalence to four herpesviruses over 30 years in Swedish children aged 9-12 years

Oral ulcers in AIDS patients frequently associated with cytomegalovirus (CMV) and Epstein-Barr virus (EBV) infections

Cytomegalovirus-associated periodontitis and Guillain-Barré syndrome

The RgpA-Kgp proteinase-adhesin complexes of Porphyromonas gingivalis inactivate the Th2 cytokines interleukin-4 and interleukin-5

Molecular evidence and clinical significance of herpesvirus coinfection in the central nervous system

Prevalence of cytomegalovirus, human herpesvirus-6, and Epstein-Barr virus in periodontitis patients and healthy subjects in the Thai population

Longitudinal monitoring of human antibody in subjects with destructive periodontal diseases

Involvement of T-lymphocytes in periodontal disease and in direct and indirect induction of bone resorption

Human cytomegalovirus enhances A. actinomycetemcomitans adherence to cells

Influenza-associated hospitalizations in the United States

Herpesviruses in localized juvenile periodontitis

Vaccines against intracellular bacterial pathogens

Fc-binding components: a virulence factor in Actinobacillus actinomycetemcomitans?

Bacterial invasion of epithelial cells and spreading in periodontal tissue

Signal transduction of lipopolysaccharide-induced osteoclast differentiation

Serum antibody response to periodontal pathogens and herpes simplex virus in relation to classic risk factors of cardiovascular disease

Bacterial internalization in periodontitis

Strong response of T cells in infants with dual infection by Epstein-Barr virus and cytomegalovirus

Polymerase chain reaction for detection of herpes simplex (HSV) DNA on mucosal surfaces: comparison with HSV isolation in cell culture

Multiple Epstein-Barr virus infections in healthy individuals

Activation of interleukin 1beta gene transcription by human cytomegalovirus: molecular mechanisms and relevance to periodontitis

EBV-1 and HCMV in aggressive periodontitis in Brazilian patients

Reciprocal transactivation between HIV-1 and other human viruses

Temporal relationships between colds, upper respiratory viruses detected by polymerase chain reaction, and otitis media in young children followed through a typical cold season

Immune stimulatory strategies for the prevention and treatment of asthma

Infection frequency of Epstein-Barr virus in subgingival samples from patients with different periodontal status and its correlation with clinical parameters

Correlation between infections with different genotypes of human cytomegalovirus and Epstein-Barr virus in subgingival samples and periodontal status of patients

Gamma-herpesvirus-induced protection against bacterial infection is transient

Detection of respiratory viruses in nasopharyngeal secretions and middle ear fluid from children with acute otitis media

Acute otitis media associated with cytomegalovirus infection in infants and children

Prevalence of human herpesviruses in patients with aggressive periodontitis

Relationship between human cytomegalovirus transcription and symptomatic apical periodontitis in Iran

Detection and quantification of herpesviruses in Kostmann syndrome periodontitis using real-time polymerase chain reaction: a case report

Epidemiology, pathogenesis, and management of human immunodeficiency virus infection in patients with periodontal disease

Socioeconomic and race ⁄ ethnic patterns in persistent infection burden among U.S. adults