key: cord-1009668-kjpegunf authors: Vu, Mai Thi; Nguyen, Anh Quynh; Nguyen, Bo Duy; Duong, Huong Thu; Van Nguyen, Quyet; Nguyen, Ha Hai; Phung, Lam Nam; Van Nguyen, Dinh title: Utility of skin testing in assessment of post-AZD1222 vaccine (AstraZeneca) allergic reactions: case series in Vietnam date: 2021-10-22 journal: Asia Pac Allergy DOI: 10.5415/apallergy.2021.11.e40 sha: 5cb46f3227a9a2dc951214510d5fc627dc716a78 doc_id: 1009668 cord_uid: kjpegunf nan To the editor, The coronavirus disease 2019 (COVID-19) pandemic is a global health emergency with more than 200 million cases and 4.5 million deaths. Vaccination is one of the main preventive measures to bring the current COVID-19 pandemic under control. Vaccines may cause adverse events; however, the majority of those adverse events are not allergic. Anaphylaxis induced by vaccines occurs approximately at a rate of 1.3:1,000,000 doses of vaccine [1] . Recent data indicate that the incidence of COVID-19 vaccine-induced anaphylaxis is higher than that of all previous vaccines. The rates of anaphylaxis caused by the novel mRNA vaccine BNT162b2 (Pfizer-BioNTech, Mainz, Germany) and mRNA-1273 (Moderna, Cambridge, MA) are 4.7:1,000,000 doses and 2.8:1,000,000 doses, respectively [2] . Early data estimates the anaphylaxis rate after AZD1222 vaccination is 7.4:1,000,000 doses [3] . Vaccine allergy is mainly caused by excipients rather than an active ingredient of the vaccine. For mRNA COVID-19 vaccines, PEG 2000 (a component of BNT162b2 and mRNA-1273) and tromethamine (in mRNA-1273) are thought to be the allergenic components, while polysorbate 80, a derivative of polyethylene glycol (PEG), is proposed as the culprit antigen of the AZD1222 vaccine [4] . However, it remains unclear whether PEG, tromethamine, or polysorbate 80 cause all of the allergic reactions to these vaccines. It is currently recommended for those at high-risk of allergic reactions, such as people with suspected or confirmed allergy to the first dose of COVID-19 vaccine to have a vaccine from a different vaccine group or to have a graded dose of the same vaccine administered [5] . Use of skin testing for COVID-19 vaccines and their components such as PEG and polysorbate in high-risk groups has been reported to confirm the diagnosis of vaccine-induced allergic reactions. However, the sensitivity, specificity, and predictive value of skin testing have not been evaluated [4] [5] [6] [7] [8] . In this case series, we examined the utility of skin testing in patients who had allergic reactions after the first dose of the AZD1222 vaccine. Ten patients with suspected allergic reactions post vaccination visited our clinics of allergy and clinical immunology at Vinmec International Hospital, Times City. Their history of allergy and clinical manifestations were examined carefully. Of those, 6 had symptoms within 4 hours, defined as immediate reactions [6] and the remaining 4 showed nonimmediate allergic reactions with onset of symptoms 5 to 12 hours after vaccination. Testing with PEG, polysorbate, and COVID-19 vaccines including AZD1222, BNT162b2, and mRNA-1273 was performed prior to the administration of a second dose of a vaccine. Selected pharmaceutical products containing PEG 3350, PEG 4000, polysorbate 20, and polysorbate 80 were used for skin testing ( Table 1) . Skin testing with PEG 3350 and PEG 4000 aimed to assess the risk of allergy to PEG 2000, a component of mRNA vaccines. PEG 3350 and PEG 4000 were used for a skin test based on the report of Banerji et al. [7] , due to an unavailability of PEG 2000 in Vietnam. Skin testing with polysorbate 20 and polysorbate 80 aimed to evaluate the risk of polysorbate allergy in the AZD1222 vaccine. Dilutions used and the order of testing is illustrated in Table 1 . Time intervals between steps were 20 minutes. Skin prick tests with undiluted AZD1222, BNT162b2, and mRNA-1273 vaccines were performed undiluted and intradermal (ID) tests were conducted using 1/10 dilutions of each vaccine. The result was read and recorded after 20 minutes (Fig. 1) . The results are presented in Table 2 . Of 6 patients (#01, #04, #05, #06, #07, and #08) who developed allergic symptoms rapidly following the AZD1222 vaccine, 4 of them (patient #01, #04, #05, and #06) had positive prick and ID tests to the AZD1222 vaccine. These 6 patients had negative skin tests to polysorbate 20 and polysorbate 80. Patient #01 then received the BNT162b2 vaccine for the second dose without prior BNT162b2 skin testing. Patient #05 had a negative skin test with PEG but a positive test with the BNT162b2 vaccine at a 1/10 dilution (ID test). This patient was then vaccinated with the BNT162b2 vaccine in graded doses without any subsequent allergic reaction (outcomes for patients #4 and #6). Of the remaining 2 patients who had suffered rapid allergic symptoms, patient #08 had a negative skin test with the AZD1222 vaccine and patient #07 refused to have a skin test with the AZD1222 vaccine because of a clear clinical history of anaphylaxis after AZD1222 vaccination. Patient #08 then received the second dose of AZD1222 in graded doses due to the immediate reaction the patient suffered after the first dose. Patient #07 had a negative skin test with BNT162b2. This patient refused skin testing with PEG, however, she tolerated the BNT162b2 vaccine as her second COVID 19 vaccine dose. Patients #09 and #10 had negative results to all of the skin tests. They tolerated well their second doses of the AZD1222 vaccine. Skin testing to polysorbate may not be sufficient to assess the allergy risk to the AZD1222 vaccine as all 6 patients with symptoms of hypersensitivity allergic reactions had negative prick tests and ID tests to polysorbate 20 and polysorbate 80. It is possible that another component of the AZD1222 vaccine is responsible for postvaccination allergic reactions. Although data examining the utility of skin testing to polysorbate in the evaluation of the risk of allergic reactions to AZD1222 is limited, there is some research which indicates there may be a role for excipient skin testing of mRNA COVID-19 vaccines [8, 9] . A positive AZD1222 vaccine skin test was associated with allergic symptoms in 6 of our cases, which may suggest that the positive predictive value of AZD1222 vaccine skin testing is significant and useful in evaluating the risk of allergy to the vaccine prior to subsequent doses of vaccines. In individuals with allergic reactions after the first dose of the AZD1222 vaccine, it is possible to change to an mRNA vaccine according to the current recommendations. Indeed, 5 out of 10 patients in our study tolerated BNT162b2 vaccination. Another of our cases had a positive 4/6 https://apallergy.org https://doi.org/10.5415/apallergy.2021.11.e40 Skin testing in patients with allergic reactions to AZD1222 vaccine ID test to BNT162b2 vaccine before the second dose and then tolerated BNT162b2 vaccine with administration of divided doses. To date, there are no data which have determined the incidence of cross-reactive allergic reaction between mRNA vaccines and the AZD1222 vaccine; however, there are several reports of cross-reactivity between polysorbate and PEG [4] . Our results suggest a role for skin testing with mRNA vaccines in patients with previous allergic reaction to AZD1222 vaccine. We conclude that skin testing to COVID-19 vaccines might be helpful in confirming the mechanism of immediate reactions to COVID 19 vaccines and in selecting an alternative vaccine for the subsequent doses. A limited role for excipients such as polysorbate and PEG in allergic reactions to COVID 19 vaccines has been observed in this case series. Further studies should be carried out to determine the precise utility of skin testing in patients with COVID-19 vaccine allergy. Written informed consent was obtained from the patients for publication of this case report and any accompanying images. This study was reviewed and approved by Vinmec Healthcare System-VinUniversity institutional ethical review board for biomedical research: No. 163.QD/VMEC. Risk of anaphylaxis after vaccination in children and adults COVID-19 vaccine safety update Centers for Disease Control and Prevention COVID-19 vaccine-associated anaphylaxis and allergic reactions: consensus statements of the KAAACI Urticaria/Angioedema/Anaphylaxis Working Group Allergy to COVID-19 vaccines: a current update COVID-19 vaccine testing & administration guidance for allergists/immunologists from the Canadian Society of Allergy and Clinical Immunology (CSACI) Middleton's allergy: principles and practice mRNA Vaccines to prevent COVID-19 disease and reported allergic reactions: current evidence and suggested approach Skin testing in patients with allergic reactions to AZD1222 vaccine First-dose mRNA COVID-19 vaccine allergic reactions: limited role for excipient skin testing Coronavirus disease 2019 vaccine hypersensitivity evaluated with vaccine and excipients allergy skin testing Acute allergic reactions to mRNA COVID-19 vaccines Authors would like to thank all patients and their families for making this possible. We gratefully thank to Clinical Professor Sheryl van Nunen for reviewing this paper.