key: cord-1005071-t0fp63w9
authors: Sugita, Kohei; Kaneko, Shuzo; Hisada, Rina; Harano, Makiko; Anno, Emi; Hagiwara, Sou; Imai, Eri; Nagata, Michio; Tsukamoto, Yusuke
title: Development of IgA vasculitis with severe glomerulonephritis after COVID-19 vaccination: a case report and literature review
date: 2022-03-11
journal: CEN Case Rep
DOI: 10.1007/s13730-022-00695-1
sha: 10e5b7afa0adb4a521c776808260796c3d61660b
doc_id: 1005071
cord_uid: t0fp63w9

With the worldwide spread of the COVID-19 vaccine program during the COVID-19 pandemic, the numbers of reported cases with new-onset or relapsed kidney disease/vasculitis such as minimal change nephrotic syndrome, immunoglobulinA (IgA) nephropathy, and IgA vasculitis (IgAV) that developed after COVID-19 vaccination are increasing. We present the case of a 67-year-old Japanese woman who developed IgAV with purpura on her extremities and trunk in the evening of the day that she received the second dose of the Pfizer-BioNTech COVID-19 vaccine. She subsequently presented with acute kidney injury and nephrotic syndrome, and a kidney biopsy performed 14 days after the second vaccination showed diffuse mesangial and endocapillary glomerulonephritis with necrotizing crescent formation, accompanied by IgA deposition. One steroid pulse plus four administrations of a monthly intravenous cyclophosphamide injection were applied, followed by oral azathioprine during oral steroid tapering. Her response to this treatment was unsatisfactory and intractable for some time. Eventually, her renal function improved and nephrotic syndrome was resolved, while microscopic hematuria and proteinuria at ~ 1 g/gCr remained at 6 months post-vaccination. Unlike the previous milder renal-involved IgAV cases following COVID-19 vaccination, our patient’s case presented severe glomerulonephritis and took a long time to recover despite intensive initial immunosuppressive treatment.

ImmunoglobulinA (IgA) vasculitis (IgAV) is an immunecomplex mediated systemic vasculitis associated with IgA. The underlying cause of IgAV is unknown, but immunological, genetic, and environmental factors are all thought to be involved [1] [2] [3] . IgAV is known to be triggered by an activation of the immune system by infection and by a variety of chemicals [1] [2] [3] . There are some reports of IgAV that developed after measles-mumps-rubella (MMR) vaccination or influenza vaccination [4] [5] [6] . With the spread of the COVID-19 pandemic and the COVID-19 vaccine programs worldwide, new-onset and relapses of primary kidney disease/vasculitis with kidney involvement that developed after COVID-19 vaccination have recently been reported [7] [8] [9] [10] [11] [12] . The association between hematuria following a COVID-19 vaccination with the onset or relapse of IgA nephropathy [13] has been attracting attention, and reports of IgAV (Henoch-Schönlein purpura with/without kidney complication) after COVID-19 vaccination are also increasing in number [14] [15] [16] [17] [18] [19] [20] [21] [22] .

Here, we present the case of a patient who exhibited IgAV immediately after receiving the second dose of a COVID-19 vaccine. Although the renal complications of all past IgAV cases were mild [14] [15] [16] [17] [18] [19] [20] [21] [22] , our patient developed severe glomerulonephritis, and it took a considerable amount of time for her recovery despite intensive initial immunosuppressive treatment.

A 67-year-old Japanese woman with a history of hypertension developed an erythematous maculopapular rash on her left leg in the evening of the same day that she received the second dose of the Pfizer-BioNTech COVID-19 vaccine by intramuscular injection. She had undergone regular medical checkups, and she had no history of urinary abnormalities or purpura in the past. She had received the first dose of the same vaccine 3 weeks prior. At approx. 5 h after the patient was administered her second vaccination, the rash had spread to both legs, upper limbs, and hips, with subcutaneous edema. The day after the second vaccination, the patient first noticed gross hematuria. On day seven after the second vaccination, the patient visited the department of internal medicine at our hospital and was examined at the department of nephrology. She was admitted to the department of nephrology on day 12.

On her admission, she showed renal impairment with serum creatinine (sCr) at 0.83 mg/dl, the estimated glomerular filtration rate (eGFR) 52.6 ml/min/1.73m 2 , urine protein (3 +), protein quantification 5.1 g/gCr, urine occult blood (3 +), and urine sediment of red blood cells > 100/ HPF (high-power fields). The results of tests for antineutrophil cytoplasmic antibody (ANCA) and cryoglobulins were negative, all other autoantibodies were negative, complement was within normal range, and IgA was within the reference range.

A kidney biopsy was performed on day 14, and a skin biopsy was performed on day 15, after which methylprednisolone 500 mg was administered intravenously for 3 days, followed by daily prednisolone 40 mg (0.8 mg/kg). After the initiation of the steroid, the patient's purpura, arthritis, and abdominal pain improved, but leg edema remained. In the specimens from the kidney biopsy, all 36 glomeruli showed mesangial or endocapillary proliferative changes (Fig. 1a, b) , 15 of which were accompanied by necrotizing cellular crescent formation (Fig. 1c) . All necrotizing cellular crescents lesions were similar, suggesting that these lesions had developed within the same time period, i.e., at the acute phase. There was no tubulointerstitial damage.

Immunofluorescent staining on a frozen section showed IgA and C3 deposition in the mesangial areas (Fig. 2 ). An electron microscopy examination revealed cell proliferative changes with an electron-dense deposit in the mesangial area. The foot process effacement of podocytes was limited to sites with endocapillary proliferative changes (Fig. 3) . A skin biopsy showed leukocytoclastic vasculitis, suggesting small-vessel vasculitis. The patient's renal function worsened to the maximum sCr value of 2.2 mg/ dl with the eGFR 18.1 ml/min/1.73m 2 , accompanied by nephrotic-range massive proteinuria; her hypoalbuminemia was minimally 2.1 g/dL.

Since the patient's nephritis with nephrotic-range proteinuria continued for 4 weeks, we started monthly intravenous cyclophosphamide (IVCY) 500 mg. The monthly IVCY was performed four times. At 4 months after the initiation of the IVCY treatment, the patient's urinary protein had decreased to 1 g/gCr and her leg edema was also improved. The IVCY was discontinued and switched to oral azathioprine. The oral glucocorticoid (prednisolone) during tapering was reduced to 10 mg/day at that point.

6 months after the start of the patient's treatment, her renal function was improved at sCr 0.65 mg/dl and eGFR 68.7 ml/min/1.73m 2 , but the urinary protein value continued to be around 1 g/gCr, and the microscopic hematuria also continued. An increased risk of IgAV within 12 weeks after measlesmumps-rubella vaccination was reported (odds ratio [OR] 3.4, 95% CI: 1.2-10.0), which was not seen with other vaccines [5] . In contrast, in a case-crossover study of various vaccines in 167 children, the risk of IgAV within 3 months after vaccination was not increased compared with the 3 months before vaccination (OR 1.6, 95% CI: 0.8-3.0) [4] . The messenger RNA (mRNA) vaccines for SARS-COv-2 such as those produced by Pfizer-BioNTech and Moderna have a pioneer mechanism of action that produces the spike proteins as viral antigens in target cells of the host. Nucleoside-modified mRNA wrapped in a lipid nanoparticle encodes the SARS-CoV-2 spike protein and releases it into the cytoplasm of target cells of the host, where it is translated by ribosomes to produce viral antigens, which are presented on the surface of Th1 cells and trigger immune responses such as the activation of killer T cells, NK cells, macrophages, or B cells to induce antibody production.

The leading immunoglobulin against COVID-19 infection is IgA [24] , which provides mucosal immunity. After COVID-19 vaccination, both IgG and IgA are elevated, but their blood kinetics are different, with IgG lasting longer [25] . Obeid et al. reported a case of relapsed IgAV following Moderna vaccination. They observed a specific increase of anti-spike IgA antibodies after COVID-19 vaccination in the patient's serum. They also reported that the patient had a transient IgA-type antinuclear antibody after vaccination, and this disappeared with steroid treatment [14] . Table 1 summarizes the cases of IgAV after vaccination that we found using a search of the Pubmed and Google Scholar databases as of January 27, 2022. The time to IgAV onset after vaccination ranged from 2 h to 20 days [14] [15] [16] [17] [18] [19] [20] [21] [22] . Urinary findings suggesting nephritis were mild and transient, and only one biopsy-proven case with mild glomerulonephritis was reported [20] . The period of IgA elevation after COVID-19 vaccination is transient and shorter [24, 25] , and the onsets of IgAN and IgAV after vaccination are earlier than that of minimal change of nephrotic syndrome [12] , suggesting that these onsets occur during IgA elevation. All acute-phase lesions in the present case seemed to be a simultaneous development, which suggests that the onset was very limited to a short period after the vaccination. However, we did not investigate the time course of the patient's serum IgA levels.

The clinical course of glomerulonephritis in our patient's case was severe, refractory, and persistent despite intensive immunosuppressive treatment. Whether postvaccination IgAV is complicated by glomerulonephritis or recovers without nephritis is an important point to discuss. This is an argument similar to the difference in mechanisms between severe/persistent and mild/transient cases of IgAV-glomerulonephritis, which has not been fully elucidated. Suzuki et al. showed that IgAV patients with nephritis had higher production and circulation of galactose-deficient (GD)IgA1 and GD IgA1-specific IgG autoantibodies compared to healthy subjects and IgAV patients without nephritis [26] . That report's findings indicate that it is possible that our present patient had large amounts of galactose-deficient (GD) IgA1 and GDIgA1specific IgG autoantibodies that were necessary to develop and maintain severe nephritis. Unfortunately, neither GD-IgA1 nor GD-IgA1-specific IgG was examined in her case.

The adverse effects of COVID-19 vaccine have been generally accepted to date. Despite the unprecedented frequency of the vaccines' use for adults worldwide, the reports of IgAV after COVID-19 vaccine are not frequent and the clinical prognoses are mild and transient in almost all cases. However, experts should be aware that some cases can progress to severe glomerulonephritis.

IgAV is a systemic vasculitis that predominantly affects children between the ages of 3 and 15 years. [1] [2] [3] In Japan at this time, the pros and cons of vaccinating infants and school children are under consideration. If COVID-19 vaccination becomes more widespread among young people in the future, the number of cases of IgAV (including severe forms) can be expected to increase, and thus epidemiological surveys as well as a clarification of the mechanisms of development are needed.

Conflict of interest All the authors have declared no competing interest.

Fully informed written consent for the publication of her case was obtained from the patient.

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