key: cord-0995185-w0j4vzaa authors: Dell’Era, Valeria; Farri, Filippo; Garzaro, Giacomo; Gatto, Miriam; Aluffi Valletti, Paolo; Garzaro, Massimiliano title: Smell and taste disorders during COVID‐19 outbreak: A cross‐sectional study on 355 patients date: 2020-06-11 journal: Head Neck DOI: 10.1002/hed.26288 sha: 3620c3708b4b22558b710d903a192d3522356a03 doc_id: 995185 cord_uid: w0j4vzaa BACKGROUND: As reported by increasing literature, a significant number of patients with SARS‐CoV‐2 infection developed smell/taste disorders. Aim of this study is to determine the prevalence and severity of these symptoms among laboratory‐confirmed SARS‐CoV‐2 patients. Secondary objective is to determine their onset/recovery time. METHODS: This cross‐sectional study was conducted from March 10 to 30, 2020 at Novara University Hospital during the COVID‐19 Italian outbreak. The 355 enrolled patients answered a questionnaire at 14th (or more) days after proven infection. RESULTS: The overall population prevalence of both smell/taste or one of the two disorders was 70%. They were first symptoms in 31 (8,7%) patients. Most patients reported a complete loss that in half of the cases (49.5%) was fully recovered after 14 days, with a median recovery time of 10 days. CONCLUSION: This study confirms a high prevalence of smell/taste disorders in COVID‐19 infection with self‐recovery for half cases after about 2 weeks. Anosmia and hyposmia, the inability to perceive smells and a reduced or altered ability respectively, as well as taste perception alterations, are occasionally associated with respiratory virus infection, including the wellknown family of coronavirus. 1 Since mid-February 2020, SARS-CoV-2, a novel coronavirus initially identified in late 2019 in China, 2 spread worldwide. As the infection was better characterized, the main symptoms of fever, cough, tiredness, and difficulty breathing were well-characterized. 3 However several anecdotal case reports, 4, 5 case series, [6] [7] [8] and surveys [9] [10] [11] [12] [13] suggested that a significant amount of patients with suspected Sars-CoV-2 infection also developed anosmia/hyposmia and/or taste alteration, and in a relevant amount of patients, these were the first symptoms of COVID-19. Most of these studies, however, base their results on presumptive or symptoms based COVID-19 diagnosis. Only one study to date have assessed a population of laboratory-confirmed patients, performing quantitative smell assessment, but without investigating taste. 14 Limited knowledge is available on prevalence, severity, and recovery time of these disorders in SARS-CoV-2 patients. The aim of this study was to investigate the prevalence and severity of smell and taste disorders in a population of laboratory-confirmed SARS-CoV-2 patients. All authors equal contribute to this paper. A secondary objective was to investigate the onset and recovery time from the same symptoms. This is a cross-sectional study conducted from March 10 to 30, 2020 at the ENT clinic of the University of Eastern Piedmont, Ospedale Maggiore della Carità in Novara, Italy. The study was carried out in accordance with the ethical standards of the Helsinki Declaration and was approved by the local ethical review board (Comitato Etico Interaziendale n.88/2020). The study included a convenience sample of SARS-CoV-2 polymerase chain reaction (PCR) confirmed cases being admitted for care or assessed and discharged during the study period. Patients with confirmed SARS-CoV-2 infection were prospectively identified and recorded. Administrative data were extracted from hospital databases and patients were offered a follow-up interview. Patients were informed about the study and asked to participate at the time of the follow-up interview (= or > 14 days from diagnosis). Interviews were carried out by two attending physicians either in person or by phone using a structured questionnaire. Inclusion criteria were: Age > 18-year old, informed consent and laboratory proven SARS-CoV-2 infection with a mild or moderate presentation of COVID-19 according to the severity classification of the Massachusetts General Hospital COVID-19 treatment guidance. 15 Exclusion criteria were: declined informed consent, a severe form of COVID-19. Interview data were anonymously collected and no reward was offered for participation. The questionnaire consisted of 22 questions, including general demographic information (age, gender), medical history (comorbidities, risks factors), SARS-CoV-2 related questions (risk exposure for occupational/family reasons, duration of infection, symptoms-fever, cough, dyspnea, fatigue or muscle pain, diarrhea or nausea) and specific information about the sense of smell and about taste (presence/ absence, 0 to 10 numeric rating scale [NRS] of smell at baseline, during infection, after 14 days and at answering time-were 0 is "no sense of smell/taste" and 10 is "normal sense of smell/taste", time of onset/recovery). The survey is available in the supplementary online material (Data S1). A clinically significant change in the smell of taste perception was defined as having a score difference equal to or greater than two points between study times. Smell and taste assessment were reported using data from the scales which have been treated as quantitative real values. Categorical variables were reported as a proportion while quantitative variables as median and ([25-75 quartile] range) where appropriate. Comparison of the baseline, worst, and 14-days smell and taste perception was performed using Friedman's test. All statistical analysis has been performed using R 3.6.3, (R Core Team [2020]. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria). A P-value <.05 was considered significant. We interviewed a total of 355 patients with laboratoryconfirmed diagnosis of SARS-CoV-2. The population had a median time from symptoms onset of 23 ([17-28] range 2-53) days. The median age was 50 years old, 192 (54%) were male. The overall population prevalence of both smell and taste or either one of the two disorders was 70% (249 of 355). Among the two study symptoms, 14 (3.9%) patients presented a smell disorder with no alteration of taste, while 12 (3.4%) patients presented with a taste disorder with no alternation of smell (but presented other SARS-CoV-2 systemic symptoms). Two patients presented with smell disorders as the sole SARS-CoV-2 symptom, while none had taste disorders as their only symptom. Smell or taste disorders were the first symptom of presentation in 31 (8.7% of entire population and 13.0%) patients. Patients characteristics of the interviewed population, and for those presenting with olfactory and taste dysfunctions are presented in Table 1 . Two hundred and thirty-seven 237 (66%) patients reported a change in smell perception greater than two points between their baseline smell and the worst smell experienced during the infection. Of these, 128 (54%) reported the smell dysfunction already present at the time of diagnosis. These patients had a baseline smell perception of 10 ( [9, 10] F I G U R E 1 Smell, A, and taste, B, perception (score) before developing symptoms (baseline), at the highest intensity of symptoms (worst) and after 2 weeks from their onset (14 days) Figure 2 shows the daily rate of recovery for both smell and taste disorders. Over the last weeks, an increasing number of authors reported a high rate of olfactory and gustatory dysfunctions in COVID-19 patients, sometimes presenting as the first symptom. [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] The main goal of the present study was to evaluate the prevalence of smell/taste disorders in a large COVID-19 positive population, composed of patients who were either asymptomatic or presenting mild or moderate symptoms. Our findings (66 and 65.4% respectively, without gender differences) are similar to those of Yan et al about a smaller sample of 59 positive patients; 10 a recent European multicenter study reported higher percentages both for smell and taste disorders, with a female prevalence. 12 Since only one study by Mao et al referred to the prevalence of chemosensory impairment in Asiatic COVID-19 population (5%), 9 our data seem to corroborate the hypothesis by Lechien et al that western positive patients (USA and Europe) may be more subject to the development of olfactory and gustatory disorders. 12 The majority of patients in this study complained of complete anosmia/ageusia, confirming the observation that COVID-19 related impairment tends to induce a severe olfactory and gustatory dysfunction. 10, 12 Of particular interest is the analysis of the timing of anosmia: the loss of smell was noted in 54% prior to diagnosis and appeared as the first symptom in 13% of cases. Recent onset of olfactory disorders may drive the physicians to treat these patients as possible COVID-19 positive: adequate precautions for the health care practitioners, PCR-testing, and home self-isolation are crucial measures to avoid that such patients continuing to accidentally act as vectors of the disease. Another pivotal issue of the research regards the recovery of olfactory and gustatory functions: 49.5% of patients reported a full regaining of both senses after 14 days since the beginning of the symptoms and this percentage improved to 62.9% at time of interview (23 days median, range 15-31), with a median recovery time of 10 days. Obviously, even if our follow-up is longer than other reports, [10] [11] [12] it remains too short to give a conclusive interpretation regarding the prognosis. Although the exact mechanism with which SARS-CoV-2 could impair smell and taste has not been definitively determined, two hypotheses appear to be plausible: damage to the olfactory epithelium, due to cell expression of angiotensin converting enzyme 2 (ACE2) receptors which act as a binding point for the virus, 16 or a direct assault on the olfactory neurons. 17 The relatively rapid and spontaneous recovery of most patients in the current and in other studies, tends to suggest an epithelial based pathogenesis, given the capability for the epithelium to quickly restore its functions after damage. [10] [11] [12] According to some Authors, the presence of 15 variants of the ACE2 gene explains many ACE2 polymorphisms and differences in expression between European and Asian populations; this finding could be a cue to investigate if different patterns may influence susceptibility and clinical features of COVID-19 infection. 18 The current study presents some limitations, shared by all similar researches. First of all, the patients did not undergo nasal endoscopy, specific imaging or objective smell assessment; these examinations would contribute to a better understanding of pathogenetic mechanisms and to define some prognostic factors about the functional recovery. Second, the evaluation tool was a self-reported smell and taste score: It has been previously demonstrated that subjective evaluation of sense of smell is quite specific but less sensitive than objective testing. 19 Moreover, the survey was rapidly developed by the Authors with the aim of collecting and sharing the most possible data in the shortest time; after such emergency contingency, additional validation is needed. Third, even if our population is quite numerous, the sampling time was short and limited to a single center; future studies on larger samples can help to clarify the prevalence of anosmia/ageusia in COVID-19 patients. Lastly, no patients in this study received specific treatment for olfactory or gustatory loss; currently no medical treatment has proved to be highly effective in post-viral anosmia. [20] [21] [22] The potential risks of immunosuppression with the use of oral and some topical steroids also need to be borne in mind, consequently we agree with most authors, who recommend against specific therapy. [4] [5] [6] [7] [8] 12, 13 A different approach could be applied to those patients with persistent olfactory disorders after complete recovery from COVID-19 and an exhaustive smell/taste assessment; in any case, further studies are needed in order to investigate potential benefits of treatment in selected cases. Beyond the limitation, this is one of the first studies to provide the prevalence, the onset time, the severity and the recovery time of COVID-19 related smell and taste disorders; the follow-up time is acceptable and the sample is various and quite representative: Novara, indeed, is a small city (100 000 inhabitants) near the border with the Lombardy Region, the epicenter of the Italian COVID-19 outbreak; at accrual time-March 30th-the positive patients in the town were almost 800, of which 355 were enrolled in the current research. The current study strongly confirms the high prevalence of olfactory and gustatory disorders in COVID-19 infection. 22 Smell and taste loss may be used as indicators of potential contagion, and early identification may help to reduce the risk of spread, especially by paucisymptomatic cases. We would like to thank you Dr. Fabio Carfagna for advice on the manuscript. Authors declare no conflicts of interest, including relevant financial interests, activities, relationships, and affiliations. The authors declare no financial and material support for the research. ORCID Valeria Dell'Era https://orcid.org/0000-0002-2171-4120 Identification of viruses in patients with postviral olfactory dysfunction. Laryngoscope Characteristics of and important lessons from the coronavirus disease 2019 (COVID-19) outbreak in China: summary of a report of 72 314 cases from the Chinese center for disease control and prevention Clinical features of patients infected with 2019 novel coronavirus in Wuhan A new symptom of COVID-19: loss of taste and smell Sudden and complete olfactory loss function as a possible symptom of COVID-19 Isolated sudden onset anosmia in COVID-19 infection. A novel syndrome? Rhinology Anosmia and ageusia: common findings in COVID-19 patients. 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