key: cord-0994767-janwx5x8 authors: Faico-Filho, K. S.; Passarelli, V. C.; Bellei, N. title: Is Higher Viral Load in SARS-CoV-2 Associated With Death? date: 2020-08-06 journal: nan DOI: 10.1101/2020.08.04.20164061 sha: 55bdc5291574f4273700b4633220e1bf49cc3d4a doc_id: 994767 cord_uid: janwx5x8 There is no proven prognostic marker or adequate number of studies in patients hospitalized for coronavirus disease 2019 (COVID19). We conducted a retrospective cohort study of patients hospitalized with COVID19 from March 14 to June 17, 2020, at Sao Paulo Hospital. SARSCoV2 viral load was assessed using the cycle threshold (Ct) values obtained from an RTPCR assay applied to the nasopharyngeal swab samples. Disease severity and patient outcomes were compared. Among the 875 patients, 50.1% (439/875) had mild, 30.4% (266/875) moderate, and 19.5% (170/875) severe disease. A Ct value of <25 (472/875) indicated a high viral load, which was independently associated with mortality (OR: 0,34; 95% CI: 0,217 to 0,533; p < 0.0001). Admission SARSCoV2 viral load is an important surrogate biomarker of infectivity and is independently associated with mortality among patients hospitalized with COVID19. The pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been responsible for causing >11 million infections around the world since December 2019 [1] . Although the severity is mild in most cases, up to 33% of the infected patients require hospitalization and almost 20% die [2, 3] . To date, several studies have focused on the risk factors for moderate to severe coronavirus disease-2019 (COVID-19), such as comorbidities, older age, and laboratory abnormalities [4, 5] . However, the effect of SARS-CoV-2 viral load on clinical outcomes remains understated. Two Chinese studies demonstrated that the viral load in hospitalized patients was higher [6, 7] and one recent American study demonstrated that higher viral loads were correlated with a higher risk of intubation and death for inpatients [8] . Nevertheless, the correlation between viral load and hospitalization risk in inpatients and outpatients remains unelucidated. Detection of SARS-CoV-2 by reverse transcription-polymerase chain reaction (RT-PCR) assay in nasopharyngeal swab specimens is the primary diagnostic method for COVID-19 [9] . Data concerning cycle threshold (Ct) values, which are inversely proportional to the amount of RNA viral copies, has been used as an inference of the viral load. Viral load can also act as a biomarker of disease severity, clinical outcome, and mortality. We conducted a retrospective analysis of SARS-CoV-2 Ct values, disease severity, and clinical outcome of 875 patients at a large university hospital in São Paulo, Brazil, between March and June 2020. . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) The copyright holder for this preprint this version posted August 6, 2020. . https://doi.org/10.1101/2020.08.04.20164061 doi: medRxiv preprint This retrospective observational cohort study involved patients who presented We used the cycle threshold (Ct) values as a semiquantitative measure of viral load. The amount of viral RNA copies present in the positive samples is inversely proportional to the corresponding Ct value. That is, the greater is the amount of viral RNA, the lower is the Ct value. Nasal swab samples were collected from the patients at admission. The RNA of the samples was purified using the Quick-RNA Viral Kit (Zymo Research, USA), according to manufacturer's instructions. The purified RNA was stored at −80°C. . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted August 6, 2020. . https://doi.org/10.1101/2020.08.04.20164061 doi: medRxiv preprint Viral detection was performed using the AgPath-ID One-Step RT-PCR reagents . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted August 6, 2020. . https://doi.org/10.1101/2020.08.04.20164061 doi: medRxiv preprint Statistical analyses were performed using the Student t-test for parametric data and the Mann-Whitney test for non-parametric data. Significance level was set at a p value of <0.05. The initial Ct values for each age group is presented in Figure 1 . We found no significant differences. . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted August 6, 2020. for patients with moderate disease was higher and significantly differed from that for patients with mild (p < 0.0001) and severe (p < 0.0001) disease, as is shown in Figure 2 . As is shown in Figure 3 , when the initial Ct was compared with the hospital discharge or death, survivors demonstrated a higher value (low RNA viral copies) than non-survivors (Ct median, 27 and 21, respectively). The initial Ct differed significantly between the two groups (p < 0.0001). Mortality rates were . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted August 6, 2020. . https://doi.org/10.1101/2020.08.04.20164061 doi: medRxiv preprint 46% (87/191) among patients with a high viral load (Ct < 25) and 22% (41/185) among patients with a low viral load. The risk of in-hospital mortality was also higher in patients with a high viral load (Ct < 25) than those with a low viral load (Ct > 24), and this association was statistically significant (OR: 0.34; 95% CI: 0.217-0.533; p < 0.0001). The analysis of viral load is applied in different endemic respiratory viruses' diseases, although a clear association between the viral load and disease severity or outcome remains unelucidated. [11, 12, 13, 14] . Nonetheless, certain viruses, such as H5N1, are considerably associated with severe . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted August 6, 2020. . https://doi.org/10.1101/2020.08.04.20164061 doi: medRxiv preprint disease, such as COVID-19. Pawestri et al. confirmed the association of the viral load with poor disease outcome. Our study included a large number of admission SARS-CoV-2 samples with a stratified clinical expression in mild (non-hospitalized), moderate, and severe (hospitalized) than described by others who did not categorize patients. Argyropoulos et al. [16] found that patients with mild Covid-19 infection presented high viral load, as was demonstrated in our study. However, their study demonstrated that hospitalized patients had a lower viral load than nonhospitalized patients, but they did not categorize the patients according to disease severity. Their small sample size probably accounts for the different results we observed in this study. Viral load distribution according to the Ct values from initial samples of our study patients followed a V curve, with mild and critically ill patients having higher viral load than hospitalized patients with a better outcome. Ct values of <25 were consistent with high viral load in patients who were more likely to die during hospitalization. Magleby et al. [17] evaluating the correlation between Ct values and clinical outcome demonstrated a significant association between higher viral load and death or intubation. The results of this study must be interpreted considering the methodological limitations. We focused on virologic aspects and did not include the effects of comorbidities, clinical symptoms, date of admission, date of sample collection, use of antivirals and antibiotics because of the heterogenous nature of the hospitalized patients and requirement of a large cohort for subgroup analysis. The duration of symptoms before testing may be an . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint this version posted August 6, 2020. . important variable, therefore, date of symptom onset is not consistently reported by medical assistants collecting samples at health services but other studies demonstrated that patients with other acute viral infections tend to present to hospital after expected peak viral load [18] . In conclusion, we found that admission SARS-CoV-2 viral load, as was determined by the Ct value, is an important surrogate epidemiological marker of infectivity that was independently associated with mortality among the patients hospitalized with COVID-19. These findings suggest that Ct values can be used to assist clinicians to identify patients at a high risk for severe outcome. . CC-BY-NC-ND 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not certified by peer review) The copyright holder for this preprint this version posted August 6, 2020. . https://doi.org/10.1101/2020.08.04.20164061 doi: medRxiv preprint Evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as coronavirus disease 2019 (COVID-19) pandemic: A global health emergency Presenting characteristics, comorbidities, and outcomes among 5700 patients hospitalized with COVID-19 in the New York City area Clinical characteristics of 138 hospitalized patients with 2019 novel coronavirus-infected pneumonia in Wuhan, China Development and validation of a clinical risk score to predict the occurrence of critical illness in hospitalized patients with COVID-19 Risk factors associated with acute respiratory distress syndrome and death in patients with Coronavirus Disease 2019 pneumonia in Wuhan, China Viral load dynamics and disease severity in patients with SARSCoV-2 in Zhejiang province Viral dynamics in mild and severe cases of COVID-19 Impact of SARS-CoV-2 Viral Load on Risk of Intubation and Mortality Among Hospitalized Patients with Coronavirus Disease WHO Laboratory testing for 2019 novel coronavirus (2019-nCoV) in suspected human cases. 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