key: cord-0990769-4fwxple4
authors: Watson, D. W.; Guy, J. S.; Stringham, S. M.
title: Limited Transmission of Turkey Coronavirus in Young Turkeys by Adult Alphitobius diaperinus (Coleoptera: Tenebrionidae)
date: 2000-05-01
journal: J Med Entomol
DOI: 10.1093/jmedent/37.3.480
sha: 9011d0a832f7e32bbce4a6afee62a80fa4606920
doc_id: 990769
cord_uid: 4fwxple4

We examined the role of lesser mealworm, Alphitobius diaperinus (Panzer), in the transmission of an enteric disease of turkeys caused by a coronavirus. Turkey coronavirus (TCV) from two sources was studied, one isolate (NC95) was embryo propagated, the second was TCV infected material from turkeys diagnosed with poult enteritis mortality syndrome (PEMS). Beetles were fed virus-infected feces mixed with chicken feed. Transmission of virus was effectively halted by surface sterilization of the beetles. Turkey poults administered beetle homogenates infected with TCV+ PEMS that had not been surface sterilized had reduced weight gains and 50% mortality. Mortality and weight gains were not effected in the NC95 group. Virus isolation procedures were performed to determine NC95 viability at varying time intervals. Beetles were dissected and the guts removed 1, 12, and 24 h after the initial viral feeding. Whole beetles were also examined for comparison. Whole beetles and beetle guts were homogenized and injected into turkey eggs for embryo propagation. Direct immunofluorescence was used to determine the presence of TCV. A. diaperinus were capable of mechanical transmission of TCV. However, only turkey embryos receiving whole beetle and beetle gut homogenates within 1 h of feeding on the virus were positive for TCV. Laboratory studies demonstrating PEMS transmission by A. diaperinus are continuing.

TURKEY CORONAVIRUS (TCV) was characterized as an emerging disease of Minnesota turkeys in 1951 (Pomeroy and Nagaraja 1991) . Manifested by a loss of appetite, weight loss, and wet droppings especially in young birds, it was an acute, highly infectious disease resulting in 25% mortality of the infected ßocks. After 1971, there occurred a dramatic drop in incidence with Ͻ1% mortality. The last TCV outbreak was controlled by depopulation, followed by disinfection of the facility, and a rest period before repopulation. Although disease has not occurred in Minnesota since 1977, it continues to be a sporadic cause of disease in other states.

Similarly, poult enteritis mortality syndrome (PEMS) emerged in turkey ßocks in North Carolina in the early part of this decade and has since spread into neighboring states. This costly disease manifests itself by an abrupt, sudden mortality in most cases Guy 1995, Barnes et al. 1996) . Surviving birds are markedly stunted, have impaired immunity, and are susceptible to other infectious diseases (Qureshi et al. 1997) . Since the disease Þrst appeared, PEMS research has focused on its cause and identifying methods of prevention, control, and treatment. Studies have demonstrated the disease is highly infectious, and although the cause of PEMS remains unknown, it is believed that the disease may be a complex of etiologic agents. For example, TCV is often, but not always, associated with the disease Barnes 1991, Guy et al. 1997) .

The lesser mealworm, Alphitobius diaperinus (Panzer), is a common pest in poultry houses. All life stages are found in poultry litter and manure, where they feed on manure, litter, meal, dead birds, and other insects (Axtell 1994, Rueda and Axtell 1997) . Consequently, this beetle has been incriminated in the transmission of several diseases and disease agents. These include Newcastle disease, avian inßuenza, infectious bursal disease, MarekÕs disease, fowl pox, Salmonellasis, Aspergillus spp., Escherichia coli, Bacillus spp., Streptococcus spp., Reovirus, Rotavirus, Eimeria (coccidiosis), tapeworms, and cecal worms (De las Casas et al. 1973 , 1976 Despins et al. 1994; McAllister et al. 1994 McAllister et al. , 1995 McAllister et al. , 1996 . Adult beetles have potential to spread disease between farms, because beetles take ßight after routine spreading of litter on Þeld soils after depopulation. Our study objective was to examine the potential of adult beetles to transmit TCV virus to healthy turkeys under laboratory conditions.

Experiments were conducted under controlled environments using commercial hatchlings and laboratory reared adult beetles. One-day-old turkeys were raised in heated brooders until 7 d of age. Turkeys were weighed and divided into eight groups of 15 birds each.

Two sources of TCV were selected for study. An embryo-propagated TCV (NC95) was obtained from PEMS-affected turkeys and embryo propagated as described by Guy et al. (1997) . Embryo-propagated TCV NC95 was administered to turkeys to establish a source of infected fecal material. The second source of TCV was fecal material obtained from a PEMS outbreak (TCVϩPEMS feces).

Adult beetles were divided into Þve groups of 200 each. The beetles were denied food and water for 3 d then allowed feed for 24 h. The negative control beetles were given chicken feed and water. The remaining four groups of beetles were fed virus-infected feces mixed with chicken feed (1:10). Two groups of beetles were given fresh NC95 infected feces. Two other beetle groups were fed TCVϩPEMS infected feces. One group from each pair of virus infected beetles was surface sterilized to remove virus particles from all but the gut, the other group was not surface sterilized (unsterilized). Beetles were surface sterilized by immersion in a solution of 50% sodium hypochlorite and Tween 80 for 10 min, then rinsed once in 70% ethanol, and three times in sterile distilled water. Whole beetle homogenates were suspended in 20 ml DulbeccoÕs minimum essential medium with 2% fetal bovine serum, 0.15 mg/ml gentamicin, and 5 g/ml amphotericin B (DMEM) tissue culture medium. After centrifugation (10 min at 2,000 ϫ g), the clariÞed homogenates were held on ice. The suspended virus supernatant was administered to the turkeys by gavage (1 ml per bird). Poult groups received one of the following eight treatments: (1) Sham-inoculated-negative control with DMEM (1 ml per bird). (2) A second negative control group was inoculated with unsterilized beetle homogenate from TCV negative beetles. Two positive control groups were inoculated with (3) NC95 TCV infected feces, and (4) TCVϩPEMS infected feces, respectively. Two additional groups were inoculated with (5) unsterilized beetle homogenate from NC95 TCV positive beetles and (6) surface sterilized beetle homogenate from NC95 TCV positive beetles. Two remaining virus treatment groups were inoculated with (7) unsterilized beetle homogenate from TCVϩPEMS beetles, and (8) surface sterilized beetle homogenate from TCVϩPEMS beetles.

Three days after inoculation, Þve birds were selected randomly from each treatment group. These birds were necropsied and examined for clinical indications of TCV infection. Bursa tissues were removed and prepared for sectioning. Bursa tissues were examined for presence of TCV by direct immunoßuorescence (FA) using a FITC-antibody conjugate prepared with antisera from TCV-hyperimmunized chickens (McNulty and Allen 1984, Guy et al. 1997) . Growth depression was measured by weighing the remaining birds on days 7, 14, and 21. The remaining birds were bled for TCV serology tests on day 21 and euthanized.

A second experiment was conducted to determine if virus remained viable in beetles for Ͻ24 h. We concluded that the other possible agents in the TCVϩPEMS source material may complicate the results and chose to use only the NC95 TCV isolate. Adult beetles were divided into seven groups of 50 each and replicated three times. Food denial and feeding was conducted as described above. Six groups of beetles were given fresh NC95 TCV infected feces. The negative control beetles were given chicken feed and water. Two groups of virus fed beetles were euthanized at time interval 1, 12, and 24 h. One group of beetles from each time interval was surface sterilized as described above. Fifty beetles from the surface sterilized group were aseptically dissected on ice to remove the gut. The second group of beetles was not surface sterilized and their bodies left whole. For each time interval, 50 beetle guts and 50 whole beetles were placed in sterile packets and immediately frozen at Ð70ЊC.

Whole beetles and beetle guts were prepared as a 10% (wt:vol) suspension in virus transport medium (DMEM), homogenized, and clariÞed by centrifugation at 2,000 ϫ g for 20 min. Fertile turkey eggs (n ϭ 21) were incubated for 23 d and inoculated into the amniotic sac with 0.2 ml of homogenated beetle suspensions (Senne 1989) . Eggs were returned to the incubator and examined daily for mortality. Embryo intestines were collected at 3 d after inoculation, frozen in optimal cutting temperature compound (Miles, Elkhart, IN), sectioned with a cryostat and Þxed in cold (Ϫ20ЊC) acetone. Tissue sections were stained using FITC-conjugated antisera speciÞc for NC95 and were examined for presence of TCV by direct immunoßuorescence (FA) as described above (Guy et al. 1997, McNulty and Allen 1984) . Antigen preparations were examined with an epißuorescence microscope. The intensity of ßuorescence was arbitrarily rated on a scale of zero (undetectable) to three (maximum ßuorescence).

Mean poult weight was calculated for each treatment. Treatment means were analyzed using one-way analysis of variance (ANOVA) (Minitab 1997) .

No mortality was observed in turkeys in the shaminoculated (DMEM) control group, or in birds inoculated with uninfected beetle homogenates. Average daily weight gain in these groups was 39.8 and 40.7 g, respectively (F ϭ 95.42, df ϭ 7, P Յ 0.005), (Table 1) . No mortality was observed in birds administered NC95 TCV infected feces but average daily weight gain was greatly reduced (23.0 g). Mortality in birds receiving TCVϩPEMS feces was 25%, with an average daily weight gain of 17.7 g.

Birds in the negative control groups were coronavirus negative in both FA and serology tests (Table 1 ). In contrast the FA tests for the positive control birds, which were administered fecal material from NC95 TCV and TCVϩPEMS sources, were 40 and 60% pos-itive, respectively. Serology tests for both positive control groups were 100% positive by day 21.

Surface sterilization of the beetles negatively impacted the survival of virus in beetles. No birds receiving surface sterilized homogenates from either NC95 or TCVϩPEMS infected beetles were found FA or serology positive (Table 1) . Birds administered unsterilized beetle homogenates (NC95) were negative in the FA test but 16% had converted serologically by day 21. Twenty percent of the birds in the TCVϩPEMS unsterilized beetle group were FA positive (day 3) and 100% had converted serologically by day 21 (Table 1) .

To better deÞne the role of A. diaperinus in the transmission of TCV the second experiment employed embryo-propagation to isolate virus. Using turkey embryo to isolate virus directly from beetle gut or whole beetle provided a more direct diagnosis of the presence of TCV by reducing competitive organisms that may interfere or confound the results. Previous studies indicate that TCV was detectable by direct immunoßuorescence (FA) by day 3 after inoculation (Guy et al. 1997) . Inoculated embryos were dissected and the intestines examined for presence of TCV. Immunoßuorescence detected the presence of virus in embryonic tissues inoculated with beetles euthanized 1 h after feeding on NC95 infected feces. No virus was found in beetle inoculated embryonic tissues for time intervals 12 and 24 h ( Table 2) . No virus was found in the negative control groups.

Mechanical transmission of a disease organism in insects may occur in two forms. The agent may be transported on the cuticle or exoskeleton of the insect, or may be ingested by the insect to be retained in a viable state as it passes through the insect gut. In this study we found that surface sterilization of the insect effectively eliminated TCV from the exoskeleton of the insect. Turkey poults infected with embryo-propagated TCV (NC95) and TCV from PEMS affected turkeys had reduced weight gains when compared with the control groups. Turkeys administered surface sterilized virus infected beetles did not suffer a reduction in weight gains, nor did they test positive by either the FA or serology. Although birds inoculated with unsterilized beetle homogenates in NC95 group did not experience a reduction in weight, one of six birds had converted serologically. Experimental studies with isolate NC95 demonstrated that the virus did not produce clinically apparent disease (Guy et al. 1999) . In our study viable virus (NC95) was either not present or perhaps below detectable levels in the beetle gut 24 h after feeding on infected feces, suggesting that the beetle gut was environmentally inhospitable for the virus. Further examination of the viability of NC95 indicated the virus remained active in beetle guts and on whole beetles (unsterilized) for 1 h but was no longer viable after 12 h. The implications of these results are evident. A. diaperinus may well be involved in the transmission of TCV within a turkey house in which an active outbreak has occurred where beetles and birds are in close contact. However, it is less likely to transmit disease upon immigrating from Þeld soils to a turkey house after the land application of litter.

The role of TCV in PEMS is less clear, because TCV may not be always present in outbreaks. Turkey poults infected with TCVϩ feces from PEMS affected turkeys had reduced weight gains when compared with the control group. Turkeys administered unsterilized beetles in the TCVϩPEMS group had reduced weight gains similar to that of the positive control group and tested positive in the FA test and serologically. Therefore, the agent(s) contributing to PEMS appear to be viable on the surface of A. diaperinus for at least 24 h. Although, the results of our second experiment indicated TCV was fastidious, some virus particles in the PEMS ϩ inoculum must have remained viable and gained purchase to be serologically detectable. The insect exoskeleton or perhaps fecal material adhering to the exoskeleton may have provided sufÞcient harborage to protect the agent(s). Although the etiology of PEMS remains unsettled this study further supports the theory that PEMS may involve a complex of organisms. Conclusive evidence of the role of A. diaperinus and other insects in the transmission of PEMS depends on the more complete characterization of the etiologic agent. Means followed by the same letter are not signiÞcantly different, P Յ 0.05, ANOVA. a n ϭ 5. b n ϭ 6. 

The biology and economic importance of the darkling beetle in poultry houses

Spiking mortality of turkeys (SMT) and related disordersÑan update

Poult enteritis mortality syndrome (SMT) and related disordersÑan update, pp. 1Ð 8. In NCSU Quarterly update to Poultry PEMS Task Force

The relationship between the lesser mealworm and avian viruses. I. Reovirus 24

Relationship between the lesser mealworm, fowl pox, and Newcastle disease virus in poultry

Transmission of enteric pathogens of turkeys by darkling beetle larva

Partial characterization of a turkey enterovirus-like virus

Antigenic characterization of a turkey coronavirus iden-tiÞed in Poult Enteritis and Mortality syndrome-affected turkeys

High mortality and growth depression are experimentally produced in young turkeys by dual infection with enteropathogenic Escherichia coli and turkey coronavirus

Reservoir competence of the lesser mealworm (Coleoptera: Tenebrionidae) for Salmonella typhimurium (Eubacteriales: Enterobacteriaceae)

Isolation of infectious bursal disease virus from the lesser mealworm, Alphitobius diaperinus (Panzer)

Reservoir competence of Alphitobius diaperinus (Coleoptera: Tenebrionidae) for Escherichia coli (Eubacteriales: Enterobacteriaceae)

Applications of immunoßuorescence in veterinary viral diagnosis

UserÕs guide, release 11. Minitab, State College

Coronaviral enteritis of turkeys (Bluecomb Disease)

Immune system dysfunction during exposure to poult enteritis and mortality syndrome agents

Arthropods in litter of poultry (broiler chicken and turkey) houses

Virus propagation in embryonating eggs

We thank L. Smith, J. Breslin, S. Denning, and the Poultry Heath Management Group, College of Veterinary Medicine, North Carolina State University for their assistance in this project. This project was supported by a grant from the North Carolina Turkey Task Force and NCO Project 6503.