key: cord-0987355-9uss9cx0
authors: Lacal, Juan Carlos; Zimmerman, Tahl; Campos, Joaquín M.
title: Choline Kinase: An Unexpected Journey for a Precision Medicine Strategy in Human Diseases
date: 2021-05-25
journal: Pharmaceutics
DOI: 10.3390/pharmaceutics13060788
sha: aa1805945163266c083466ee42bb3d6b2400639c
doc_id: 987355
cord_uid: 9uss9cx0

Choline kinase (ChoK) is a cytosolic enzyme that catalyzes the phosphorylation of choline to form phosphorylcholine (PCho) in the presence of ATP and magnesium. ChoK is required for the synthesis of key membrane phospholipids and is involved in malignant transformation in a large variety of human tumours. Active compounds against ChoK have been identified and proposed as antitumor agents. The ChoK inhibitory and antiproliferative activities of symmetrical bispyridinium and bisquinolinium compounds have been defined using quantitative structure–activity relationships (QSARs) and structural parameters. The design strategy followed in the development of the most active molecules is presented. The selective anticancer activity of these structures is also described. One promising anticancer compound has even entered clinical trials. Recently, ChoKα inhibitors have also been proposed as a novel therapeutic approach against parasites, rheumatoid arthritis, inflammatory processes, and pathogenic bacteria. The evidence for ChoKα as a novel drug target for approaches in precision medicine is discussed.

Alteration of cell metabolism is a frequent event in human diseases [1] . In some instances are a necessary requirement, such as in the case of cancer onset and progression. A typical feature of cancer cells is an increased metabolic rate which supports unregulated growth and higher duplication rates. In keeping with this requirement, aerobic glycolysis, glutamine catabolism and lipid metabolism are frequently up-regulated in tumours [1] . As a key event, the pathways involved in the generation of the major phospholipids, such as cytidine diphosphate-choline (CDP-choline) and cytidine diphosphate-ethanolamine (CDP-ethanolamine), are frequently altered in human tumours. One of the enzymes that regulate these pathways is Choline kinase α (ChoKα). Evidence that ChoKα plays a critical role in many human diseases is increasingly being accumulated, and ChoKα has become the focus of a targeted therapeutic strategy [2] .

As a consequence, small molecule inhibitors and small interfering RNA (siRNA) that interfere with the function of ChoKα have been designed. These reagents have proven to be effective and selective anticancer drugs [2] . We describe here how the most active antiproliferative and antitumoral agents against ChoK were designed by relying on

In humans, two genes code for the enzymatic activity responsible for the generation of phosphorylcholine (PCho) designated as CHKA and CHKB. Located in chromosomes 11q13.1 (CHKA) and 22q13.33 (CHKB), they code for proteins of ca. 50 kDa (ChoKα) and 45 kDa (ChoKβ, 395 amino acids) [4] [5] [6] . The CHKA gene originates by differential splicing ChoKα1 (52 kDa, 457 amino acids) and ChoKα2 (50 kDa, 439 amino acids) ( Figure 1 ). active antiproliferative and antitumoral agents against ChoK were designed by relying on quantitative structure-activity relationships (QSARs) and structural parameters. The initial framework of these inhibitors was based on symmetrical bispyridinium and bisquinolinium structures. ChoKα inhibitors have also been proposed as a novel therapeutic approach against malaria, rheumatoid arthritis, pathogenic bacteria and inflammatory processes [3] . The aim of this review is to recount the history of the development of ChoKα inhibition therapies against human diseases and to summarize the most striking findings in this field. It is a fascinating journey from bench to the bedside. In addition, there are many paths yet to be fully explored in areas of illnesses other than cancer.

In humans, two genes code for the enzymatic activity responsible for the generation of phosphorylcholine (PCho) designated as CHKA and CHKB. Located in chromosomes 11q13.1 (CHKA) and 22q13.33 (CHKB), they code for proteins of ca. 50 kDa (ChoKα) and 45 kDa (ChoKβ, 395 amino acids) [4] [5] [6] . The CHKA gene originates by differential splicing ChoKα1 (52 kDa, 457 amino acids) and ChoKα2 (50 kDa, 439 amino acids) ( Figure 1 ).

ChoK characteristics. ChoK catalyzes the phosphorylation of choline, rendering phosphorylcholine. This reaction requires Mg 2+ and ATP. In humans, two distinct genes, CHKA and CHKB, code for the ChoK enzymes. The locus of these genes is indicated. CHKA generates ChoKα1 and ChoKα2 by differential splicing. Their molecular weight and size are included as well as their intracellular location.

There is an overall sequence identity of 56% between ChoKα and ChoKβ proteins. Nevertheless, they seem to serve different metabolic and biological functions. ChoKα is essential for mouse [7] and plant [8] embryo development. A ChoKα knockout cannot be rescued by ChoKβ [7] . Meanwhile, ChoKβ is dispensable in mice and a ChoKβ Knockout (ChoKβ KO) leads to a non-lethal, rostrocaudal muscular dystrophy and forelimb deformity due to decreased levels of phosphatidylcholine [9] that can be rescued by ChoKα [10] . More recently, the role of ChoKβ in bone homeostasis in mice has been demonstrated [11] . This result is consistent with the fact that pharmacological inhibition of ChoK activity in human osteoblasts results in defective mineralization in vitro [12] . In humans, mutations in the CHKB gene lead to similar pathology [13] . Finally, in Arabidopsis, there are four Choline-Ethanolamine Kinase (CEK) genes related to choline (Cho) and ethanolamine (Etn) phosphorylation, with different roles in metabolism and development [14] .

Additional enzymatic analyses revealed differential activity patterns for ChoKα and ChoKβ, φυρτηερ συππορτινγ different physiological roles.

Although both have Cho kinase and Etn kinase activities under in vitro conditions, in whole cells assays they divert. ChoKα still has both activities but ChoKβ has only the ethanolamine kinase activity [15] . Furthermore, ChoKβ does not compensate for de- Figure 1 . ChoK characteristics. ChoK catalyzes the phosphorylation of choline, rendering phosphorylcholine. This reaction requires Mg 2+ and ATP. In humans, two distinct genes, CHKA and CHKB, code for the ChoK enzymes. The locus of these genes is indicated. CHKA generates ChoKα1 and ChoKα2 by differential splicing. Their molecular weight and size are included as well as their intracellular location.

There is an overall sequence identity of 56% between ChoKα and ChoKβ proteins. Nevertheless, they seem to serve different metabolic and biological functions. ChoKα is essential for mouse [7] and plant [8] embryo development. A ChoKα knockout cannot be rescued by ChoKβ [7] . Meanwhile, ChoKβ is dispensable in mice and a ChoKβ Knockout (ChoKβ KO) leads to a non-lethal, rostrocaudal muscular dystrophy and forelimb deformity due to decreased levels of phosphatidylcholine [9] that can be rescued by ChoKα [10] . More recently, the role of ChoKβ in bone homeostasis in mice has been demonstrated [11] . This result is consistent with the fact that pharmacological inhibition of ChoK activity in human osteoblasts results in defective mineralization in vitro [12] . In humans, mutations in the CHKB gene lead to similar pathology [13] . Finally, in Arabidopsis, there are four Choline-Ethanolamine Kinase (CEK) genes related to choline (Cho) and ethanolamine (Etn) phosphorylation, with different roles in metabolism and development [14] .

Additional enzymatic analyses revealed differential activity patterns for ChoKα and ChoKβ, φ υ ρ τ η ε ρ σ υ π π o ρ τ ι ν γ different physiological roles. Although both have Cho kinase and Etn kinase activities under in vitro conditions, in whole cells assays they divert. ChoKα still has both activities but ChoKβ has only the ethanolamine kinase activity [15] . Furthermore, ChoKβ does not compensate for decreased phosphatidylcholine (PC) biosynthesis in ChoKα +/− heterozygous mice. ChoKα production is sufficient to maintain normal PC levels in most tissues, but not in limb muscles of ChoKβ KO mice. Furthermore, phosphatidylethanolamine (PE) levels are unaffected in ChoKβ KO mice, a Pharmaceutics 2021, 13, 788 3 of 28 result which suggests that PE homeostasis is fully maintained when the ChoKα protein is intact [9] . Again, in Arabidopsis, substrate specificity for Cho and Etn are distinct for the four CEK enzymes [14] and resembles the situation described in mammalian cells [15] .

The crystal structure of human ChoK demonstrates that this enzyme forms a dimer (doi:10.2210/pdb2CKQ/pdb; doi:10.2210/pdb5EQY/pdb) [16, 17] . This structural feature, confirmed in enzymatic analysis may have important biochemical and biological consequences since different levels of enzymatic activity for homo-and heterodimers have been reported, with the ChoKα homodimer as the most active and the ChoKβ homodimer as the least active [18, 19] . Furthermore, a balance of expression of the two isoforms may have a differential effect on the regulation of the cell cycle [18, 19] . Dimerization ratios may therefore serve as a complex regulatory mechanism for enzyme function, though further work has to be done to clarify this system.

ChoKα is overexpressed in a large diversity of human tumours including breast [20] , lung [21] [22] [23] , colorectal [21, 24] , bladder [25] , prostate [21, [26] [27] [28] , ovary [29] , endometrial [30] , pancreas [31] , liver [32] [33] [34] [35] , esophagus [36] , and T-cell lymphoma [37] . ChoKα overexpression has also been reported in osteosarcoma tumour-derived cancer cells [12] , Hepatitis B Virus (HBV)-induced hepatocarcinomas [38, 39] , breast, colon-and liver-derived cancer cells [40] , glioblastoma- [41] and glioma-derived cell lines [42] , pancreatic tumourderived cell lines [31, 43] and T-cell acute lymphoblastic leukemia (T-ALL) primary cells and commercial cell lines [44] . In addition, ChoKα expression levels appears to be a marker of cancer prognosis, aggressiveness or metastasis, in lung [22, 23] , breast [45, 46] , bladder [25] , prostate [26, 27] , ovary [29] , colorectal [24] , esophageal squamous cell carcinomas [36] , liver [34, 35] and B-Cell lymphomas [47, 48] . In breast cancer, it has been associated with ER − status, increased invasiveness, and drug resistance [20, 45, 46, 49, 50] .

Since ChoKα has an established role in the onset and progression of human cancers, this enzyme was proposed as a novel therapeutic target for cancer [2] . Therefore, a program for the design and synthesis of specific inhibitors (see Section 4 below) was established. siRNA attenuation of ChoK production was also used to support the proof of concept [51] [52] [53] . Thus, small molecules and specific siRNAs with potent antitumor activities both in vitro and in experimental animal models have been developed.

To further support the importance of ChoKα as a target for cancer therapy, ChoKα inhibition has been used in combinatorial regimes and showed a potent antitumor effect that synergizes with 5-FU in breast and colon cancer cells [54] [55] [56] and reverses resistance to TRAIL in colorectal [57] and ovarian cancer cells [58] . Similar synergic effects have been obtained with pancreatic ductal adenocarcinoma-derived cancer cells with gemcitabine, 5-FU or oxaliplatin [31] , and for lung cancer cells with acid ceramidase inhibitors [59] or cisplatin (Lacal, unpublished) .

PC synthesis is an absolute requirement of cancer cells in order to proliferate. Thus, inhibition of ChoKα has been shown to have therapeutic effects in a variety of tumorderived cancer cell lines and tumor xenografts [2, 3] . Blocking this metabolic pathway has been shown to be non-toxic to normal, primary cells as well as immortalized nontumorigenic cells, implying that different responses are activated in tumorigenic and non-tumorigenic cells in different cell systems [60] [61] [62] [63] .

A large number of studies use magnetic resonance spectroscopy (MRS), high-resolution magic angle spinning MRS (HR-MAS MRS) or positron emission tomography (PET) to determine ChoK levels or their products as biomarkers in cancer diagnosis and prognosis have been reviewed recently. Therefore, although this is a very important field in development, these techniques will not be the subject of this review.

The cost of drug discovery is far too high to be led solely by trial and error. In computer-aided drug design, quantitative structure-activity relationship (QSAR) studies are an effective and useful approach. A QSAR study attempts to understand differences in the biological activities of a group of congeners in terms of molecular changes induced by substituent changes.

The most potent ChoK inhibitor known in the early 1990s was hemicholinium-3 (1, HC-3) with an IC 50 for ChoK inhibition ex vivo of 500 µM using recombinant ChoK, and an IC 50 against the human HT-29 cell line of 2.5 mM [64] . A high affinity paralyzing respiratory effect is induced by HC-3 [65] , which is a competitive inhibitor of Cho transport. As a result, 1 cannot be used as an antiproliferative agent in vivo. Improving the potency and diminishing the toxicity of 1, by changing its specificity and antiproliferative activity, was the first step in developing potential anticancer drugs that target ChoK. The ChoK inhibitory and antiproliferative activities of symmetrical bispyridinium and bisquinolinium compounds have been defined using quantitative structure-activity relationships (QSARs) and structural parameters. We will review this strategy in the following sections.

Two choline-like chains comprising quaternary groups are bound to the central biphenyl system in the HC-3 structure, forming oxazonium rings in solution [65] . The 1,4-oxazonium moieties of 1 were replaced with pyridinium rings with various substituents at position 4 in order to investigate their electronic effects. The substitution could be carried out at position 2 of the pyridinium ring, in which the possible electronic effects (inductive and mesomer) could also be operational; however, in the latter position, the sterical effect might have masked or altered the electronic effect. Therefore, in our earlier sudies we eliminated the possibility of substitution at the position adjacent to the N + . We have used the 1,2-ethylene(bisbenzyl) moiety as a linker to maintain the number of atoms between the two positively charged nitrogen atoms of 1 (Figure 2 ). agnosis and prognosis have been reviewed recently. Therefore, although this is a very important field in development, these techniques will not be the subject of this review.

The cost of drug discovery is far too high to be led solely by trial and error. In computer-aided drug design, quantitative structure-activity relationship (QSAR) studies are an effective and useful approach. A QSAR study attempts to understand differences in the biological activities of a group of congeners in terms of molecular changes induced by substituent changes.

The most potent ChoK inhibitor known in the early 1990s was hemicholinium-3 (1, HC-3) with an IC50 for ChoK inhibition ex vivo of 500 μM using recombinant ChoK, and an IC50 against the human HT-29 cell line of 2.5 mM [64] . A high affinity paralyzing respiratory effect is induced by HC-3 [65] , which is a competitive inhibitor of Cho transport. As a result, 1 cannot be used as an antiproliferative agent in vivo. Improving the potency and diminishing the toxicity of 1, by changing its specificity and antiproliferative activity, was the first step in developing potential anticancer drugs that target ChoK. The ChoK inhibitory and antiproliferative activities of symmetrical bispyridinium and bisquinolinium compounds have been defined using quantitative structure-activity relationships (QSARs) and structural parameters. We will review this strategy in the following sections.

Two choline-like chains comprising quaternary groups are bound to the central biphenyl system in the HC-3 structure, forming oxazonium rings in solution [65] . The 1,4-oxazonium moieties of 1 were replaced with pyridinium rings with various substituents at position 4 in order to investigate their electronic effects. The substitution could be carried out at position 2 of the pyridinium ring, in which the possible electronic effects (inductive and mesomer) could also be operational; however, in the latter position, the sterical effect might have masked or altered the electronic effect. Therefore, in our earlier sudies we eliminated the possibility of substitution at the position adjacent to the N + . We have used the 1,2-ethylene(bisbenzyl) moiety as a linker to maintain the number of atoms between the two positively charged nitrogen atoms of 1 ( Figure 2 ). The compounds were screened in an ex vivo system using the human recombinant ChoK as a target. We determined the effect of our compounds on the ChoK enzymatic The compounds were screened in an ex vivo system using the human recombinant ChoK as a target. We determined the effect of our compounds on the ChoK enzymatic activity regardless of other confounding factors such as permeability into cells, special cellular conditions, intracellular modifications of compounds or compartmentalization. ChoK inhibitors were also tested on the HT-29 cell line to see how they affected cell proliferation in tumor cells [66] . This cell line was developed from a colon adenocarcinoma, one of the most common solid human cancers that are resistant to chemotherapy, making these cells suitable for the production of new antitumor drugs. The Ghose-Crippen modified atomic contribution method (ATOMIC5 option) [67] of the PALLAS 2.0 software [68] was used to measure the clog P (calculated log P) values of the bis-salts. π spacer is the linker sub- activity regardless of other confounding factors such as permeability into cells, special cellular conditions, intracellular modifications of compounds or compartmentalization. ChoK inhibitors were also tested on the HT-29 cell line to see how they affected cell proliferation in tumor cells [66] . This cell line was developed from a colon adenocarcinoma, one of the most common solid human cancers that are resistant to chemotherapy, making these cells suitable for the production of new antitumor drugs. The Ghose-Crippen modified atomic contribution method (ATOMIC5 option) [67] of the PALLAS 2.0 software [68] was used to measure the clog P (calculated log P) values of the bis-salts. πspacer is the linker substituent constant and πcat head is the -CH2-C6H4-(CH2)n-C6H4-CH2-group substituent constant, determined with the PALLAS 2.0 program [67] Ghose-Crippen modified atomic contribution method (ATOMIC5 option) [67] .

The activity against the HT-29 cell line is, on average, higher than the activity against ChoK. Thus, the symmetrical bisquaternized salts may be affecting a different part of the pathway in this tumor cell line (see Tables 1-3 ). Electronic parameter for resonance effects (ref. [69] ). c Predicted by using the Ghose-Crippen modified atomic contribution system (ATOMIC5 option, ref. [67] ) of the PALLAS 2.0 program [68] . d πR4 = clog PR4 − clog PH; clog P values have been calculated using the CDR option of PALLAS 2.0 program. e See ref. [70] . f These values were estimated by 13 C NMR spectroscopy (see ref. [71] ).

All of the R 4 substituents are either electron-releasing, neutral, or electron-withdrawing (-NMe2, -NH2, -CH2OH, -Me, -COOH, -C≡N). The electronic parameters for resonance effect (σR) were published by Charton [69] , and the following correlation Equation (1) 

where, pIC50 = −log IC50, bearing in mind that the higher the value of pIC50 the more potent is the compound, n is the number of compounds, r is the correlation coefficient, s is the standard deviation, F is the F ratio between the variances of observed and calculated activities, and data within parentheses are standard errors of estimate. [69] ). c in vitro assay carried out on the HT-29 cell line, Predicted by using the Ghose-Crippen modified atomic contribution system (ATOMIC5 option, ref. [67] ) of the PALLAS 2.0 program [68] . d π R4 = clog P R4 − clog P H ; clog P values have been calculated using the CDR option of PALLAS 2.0 program. e See ref. [70] . f These values were estimated by 13 C NMR spectroscopy (see ref. [71] ). tron-releasing effect than the -NH2 or -NMe2 groups. As a result, endocyclic am groups like pyrrolidino, piperidino, and perhydroazepino may have a stronger e tron-releasing effect. The N-methylanilino and the N-diallyl groups were also tes Furthermore, when compared to the -NH2 and -NMe2 groups, their higher lipophilici can aid in the antiproliferative activities of even more active compounds [70] . 13 C NMR spectroscopy was used to estimate the unknown σR descriptors for the allylamino, pyrrolidino, piperidino, perhydroazepino, and N-methylanilino moie [71] . The totality of the ten compounds synthesized until then give rise to Equation ( MN58b is a member of the first generation of HC-3 derivatives that were used prototype for studying ChoKα effects in both normal and tumor cells [2] .

We chose structures with the following characteristics to investigate the poten effect of lipophilicity on ChoK inhibition in ex vivo conditions [72] :

(a) Cationic heads with a "zero electronic effect", i.e., hydrogen at position 4, that al the positive charge to be dispersed to a large extent. We used unsubstituted qu linium and isoquinolinium rings to achieve this. (b) Aralkyl spacers with different number of methylene groups.

ChoK inhibition activity is found to correlate with lipophilicity for these c pounds, as shown in Equation (3) On the basis of Equation (3), we propose that hydrophobic interactions between bisalts 13-20 and ChoK can occur. The QSAR greatest contribution is that it has provi a comprehensive and reasonably complete quantitative understanding of the role of drophobicity in drug action [73] . Hydrophobicity is related not only to absorption distribution but also to the interactions with ChoK active site. Apart from electrost interactions, it appears that the cationic heads and spacers between the two positive trogen atoms, hydrophobilically bind to the enzyme more strongly. Non-covalent in actions in aqueous solution are dominated by hydrophobic interactions. At hydropho surfaces, loosely connected water molecules have a degree order and are therefore in undesirable entropic degree. The interaction of a drug hydrophobic areas with its bi ing site releases the organized water molecules, resulting in an increase in entropy. planar quinolinium and isoquinolinium cations have been shown to bind to artificial ceptors, with aromatic rings, more strongly than the alkylammonium cations [74] . Comp.

a All values are the mean of two independent determinations performed in duplicate. b Recombinant human ChoK was used as a target, Predicted by using the Ghose-Crippen modified atomic contribution system (ATOMIC5 option, ref. [67] ) of the PALLAS 2.0 program [68] . c in vitro assay carried out on the HT-29 cell line, ND: Not determined. Our QSAR studies revealed that the efficacy of inhibitors is determined by π at a particular location on the molecules rather than the overall log P. Equation (5) suits very well with the use of these site-specific π parameters: p(IC50)ex vivo = 0 In order to obtain Equation (5), we had to accept that for compounds 2-12, the πcat head was equal to zero (π2H due to the H-2 and H-3 protons of the pyridinium moiety), a All values are the mean of two independent determinations performed in duplicate. b Recombinant human ChoK from yeast was used as a target, σ R : Electronic parameter for resonance effects (ref. [69] ). c in vitro assay carried out on the HT-29 cell line, Predicted by using the Ghose-Crippen modified atomic contribution system (ATOMIC5 option, ref. [67] ) of the PALLAS 2.0 program [68] . d π R4 = clog P R4 − clog P H ; clog P values have been calculated using the CDR option of the PALLAS 2.0 program. e We have used the acetamido value instead of the pivaloylamino group σ R value because the latter value was unavailable (ref. [69] ).

All of the R 4 substituents are either electron-releasing, neutral, or electron-withdrawing (-NMe 2 , -NH 2 , -CH 2 OH, -Me, -COOH, -C≡N). The electronic parameters for resonance effect (σ R ) were published by Charton [69] , and the following correlation Equation (1) emerges from data of Table 1 [64, 72] :

where, pIC 50 = −log IC 50 , bearing in mind that the higher the value of pIC 50 the more potent is the compound, n is the number of compounds, r is the correlation coefficient, s is the standard deviation, F is the F ratio between the variances of observed and calculated activities, and data within parentheses are standard errors of estimate. The inhibitory potency of the compounds is unrelated to their inductive effect [64] , but is well correlated with their resonance effect. This is in line with traditional chemical principles. The positively charged ring nitrogen is in direct conjugation with R 4 . The higher the resonance effect of R 4 , the better the delocalization of the positive charge. Following these findings, researchers looked for a substituent with a higher electron-releasing effect than the -NH 2 or -NMe 2 groups. As a result, endocyclic amino groups like pyrrolidino, piperidino, and perhydroazepino may have a stronger electron-releasing effect. The Nmethylanilino and the N-diallyl groups were also tested. Furthermore, when compared to the -NH 2 and -NMe 2 groups, their higher lipophilicities can aid in the antiproliferative activities of even more active compounds [70] . 13 C NMR spectroscopy was used to estimate the unknown σ R descriptors for the diallylamino, pyrrolidino, piperidino, perhydroazepino, and N-methylanilino moieties [71] . The totality of the ten compounds synthesized until then give rise to Equation (2):

MN58b is a member of the first generation of HC-3 derivatives that were used as a prototype for studying ChoKα effects in both normal and tumor cells [2] .

We chose structures with the following characteristics to investigate the potential effect of lipophilicity on ChoK inhibition in ex vivo conditions [72] :

(a) Cationic heads with a "zero electronic effect", i.e., hydrogen at position 4, that allow the positive charge to be dispersed to a large extent. We used unsubstituted quinolinium and isoquinolinium rings to achieve this. ChoK inhibition activity is found to correlate with lipophilicity for these compounds, as shown in Equation (3):

On the basis of Equation (3), we propose that hydrophobic interactions between the bisalts 13-20 and ChoK can occur. The QSAR greatest contribution is that it has provided a comprehensive and reasonably complete quantitative understanding of the role of hydrophobicity in drug action [73] . Hydrophobicity is related not only to absorption and distribution but also to the interactions with ChoK active site. Apart from electrostatic interactions, it appears that the cationic heads and spacers between the two positive nitrogen atoms, hydrophobilically bind to the enzyme more strongly. Non-covalent interactions in aqueous solution are dominated by hydrophobic interactions. At hydrophobic surfaces, loosely connected water molecules have a degree order and are therefore in an undesirable entropic degree. The interaction of a drug hydrophobic areas with its binding site releases the organized water molecules, resulting in an increase in entropy. The planar quinolinium and isoquinolinium cations have been shown to bind to artificial receptors, with aromatic rings, more strongly than the alkylammonium cations [74] .

The electron-releasing potential of the substituent at position 4 of the heteroaromatic cationic head (Table 1 ) and the lipophilicity of the bissalts (Table 2 ) had a significant effect in ChoK inhibition. Therefore, in order to improve the inhibitory efficacy, we combined these two properties in the same molecule and prepared compounds 21 and 22 (Table 3) . The -NH 2 group is an electron-releasing group that should be strong, while the pivaloylamino moiety should be weak. We used the corresponding value of the very similar acetamido group instead (σ R = −0.35) [69] because the latter σ R value was unavailable. The results met our standards, so we tried to link the ChoK inhibitory potency with the two descriptors, resulting Equation (4), in which all the compounds of Tables 1-3 were included: p(IC 50 ) ex vivo = 4.44 − 0.73 (±0.14) σ R + 0.12 (±0.04) clog P n = 19, r = 0.836, s = 0.241, F 2,16 = 18.61 (significance at α < 0.001)

Our QSAR studies revealed that the efficacy of inhibitors is determined by π at a particular location on the molecules rather than the overall log P. Equation (5) suits very well with the use of these site-specific π parameters:

In order to obtain Equation (5), we had to accept that for compounds 2-12, the π cat head was equal to zero (π 2H due to the H-2 and H-3 protons of the pyridinium moiety), while for bisquinolinium and bisisoquinolinium structures 13-22, π cat head is 1.27. π spacer is the -CH 2 -C 6 H 4 -(CH 2 ) n -C 6 H 4 -CH 2 -group substituent constant, which ranges between 5.14 (n = 0 of the spacer) and 6.28 (n = 3 of the spacer). The fact that π spacer has a higher coefficient and values than π cat head leads to the inference that the former is much more significant than the latter in terms of hydrophobicity. Finally, the positive π-term coefficients indicate that hydrophobic moieties and electron-donating groups support ChoK inhibitory action, at least within the context of spacers and heteroaromatic rings used.

The final research focused on two aspects: (a) the impact that a variation of the linker that links the quinolinium cations with electron-releasing groups at position 4 with other [66] . Tables 4-6 show the biological results of compounds 23-63, which correspond to series A, B, and C, as a function of the group at position 7 of the quinolinium ring. significant than the latter in terms of hydrophobicity. Finally, the positive π-term coefficients indicate that hydrophobic moieties and electron-donating groups support ChoK inhibitory action, at least within the context of spacers and heteroaromatic rings used.

The final research focused on two aspects: (a) the impact that a variation of the linker that links the quinolinium cations with electron-releasing groups at position 4 with other different groups at positions 3, 7, and 8 of the heterocycle (compounds 23-63) would have on the ex vivo human inhibitory activity; and (b) the impact of the factors that regulate the antiproliferative properties of such compounds [66] . Tables 4-6 show the biological results of compounds 23-63, which correspond to series A, B, and C, as a function of the group at position 7 of the quinolinium ring. Lipophilicity, molar volume, and steric bulk are all factors that influence molar refractivity (MR) [75] . To get rational values for the regression coefficients of the resulting QSAR equations, the MR values are usually scaled by a factor of 0.1. With the support of 3D structures, the importance of MR in QSAR equations of certain ligand-enzyme interactions were interpreted. These studies revealed that π-modeled substituents bind in a hydrophobic space. Interactions between these substituents with a polar surface will give rise to a positive sign of MR in a QSAR equation [76, 77] , while a negative sign or a nonlinear relationship indicates a limited area or steric hindrance at this binding site [75] . Lipophilicity, molar volume, and steric bulk are all factors that influence molar refractivity (MR) [75] . To get rational values for the regression coefficients of the resulting QSAR equations, the MR values are usually scaled by a factor of 0.1. With the support of 3D structures, the importance of MR in QSAR equations of certain ligand-enzyme interactions were interpreted. These studies revealed that π-modeled substituents bind in a hydrophobic space. Interactions between these substituents with a polar surface will give rise to a positive sign of MR in a QSAR equation [76, 77] , while a negative sign or a nonlinear relationship indicates a limited area or steric hindrance at this binding site [75] . Lipophilicity, molar volume, and steric bulk are all factors that influence molar refractivity (MR) [75] . To get rational values for the regression coefficients of the resulting QSAR equations, the MR values are usually scaled by a factor of 0.1. With the support of 3D structures, the importance of MR in QSAR equations of certain ligand-enzyme interactions were interpreted. These studies revealed that π-modeled substituents bind in a hydrophobic space. Interactions between these substituents with a polar surface will give rise to a positive sign of MR in a QSAR equation [76, 77] , while a negative sign or a nonlinear relationship indicates a limited area or steric hindrance at this binding site [75] . Equation (6) was obtained after taking into account the volume effects (MR 8 ) (the subscript refers to the location of the substituent), the measured global lipophilicity (clog P), the linker substituent constant, and the electronic parameters (σ R ) of the R 4 substituent for antiproliferative activity: The following points should be illustrated from Equation (6): (a) Since the coefficient of MR 8 is a squared negative expression, the existence of a methyl group at position 8 is detrimental to antiproliferative activity; thus, a hydrogen atom at this position is preferable because it has a lower MR value; (b) Lipophilicity contributes to the antiproliferative activity in two ways: on the one hand, it has a global contribution (clog P), and on the other, it has a particular contribution at a specific location on the molecules (π linker ). Since both descriptors are orthogonal, their inclusion in the QSAR equation is justified. Since π linker has a higher relative contribution in Equation (6) than clog P, it is possible that favorable hydrophobic interactions between the enzyme and the linker modulate the inhibitor-ChoK coupling. While raising the global lipophilicity and the linker lipophilicity would increase antiproliferative activity, solubility was the reason for limiting the spacers to 3,3 -, 4,4 -biphenyl, and 4,4 -bibenzyl moieties. Equation (7) is obtained when the experimental p(IC 50 ) HT-29 values are compared with the theoretical ones determined by Equation (7): 

Since it obtained the best experimental results, compound RSM-932A was selected for further study. It acts similarly to MN58b in vitro by inducing cell cycle arrest in nontumorigenic cells and apoptosis in tumor cells [60] [61] [62] [63] . RSM-932A has no detectable toxicity in mice at highly effective doses that lead 77 percent tumor growth inhibition in in vivo conditions [78] . Indeed, RSM-932A (also known as TCD-717) was the first ChoKα inhibitor to be studied in humans, in a phase I clinical trial [79] .

Other modifications have been carried out to give rise to newly designed structures 64-68 ( Figure 3) .

Symmetrical bis-charged structures containing minor modifications in the spacer have been described [80] . Unfortunately, because of their structural homology to choline, double positively charged chemotypes are correlated with substantial toxicity due to off-target activities [81] . The lead series described by Zech et al. [81] , on the other hand, relies heavily on basic amines found in methyldiazepanes to bind to the choline site. A primary hydrogen bond is added by the protonated 1' nitrogen. It is important to remember that depending on local conditions like pH, the methyldiazepane moiety may be transiently protonated or present as a free base. Furthermore, diazepane is used in a wide variety of licensed medications, mainly to boost solubility or ADME parameters. In most cases, this moiety does not pose a significant toxicity risk. Compound 68 induced apoptosis at low µM concentrations in the ChoKα expressing line MDA-MB-415.

From their synthesis to the molecular basis of their binding mode, a review in the field of ChoK inhibitors was published [82] . The first library of asymmetric bispyridinium derivatives was reported by Rubio-Ruiz et al. [82] . To investigate potential new binding modes to the enzyme. 4(4-Chloro-N-methylanilino)pyridinium, 1-benzyl-4-(dimethylamino)pyridinium, and 4-pyrrolidinopyridinium fragments were combined in these structures, with both positively charged moieties linked with variable linkers. The plasticity of the choline binding site, the discovery of new exploitable binding sites, and the allosteric properties of this enzyme are all emphasized as strategies for developing inhibitors and their selectivity on ChoKα over ChoKβ. Rubbini [80] ), symmetrical uncharged (66 [81] ), unsymmetrical mono-charged ChoKα inhibitors (67, 68 [82] ) and bis-charged ChoKα inhibitors (69 [83] ).

Symmetrical bis-charged structures containing minor modifications in the spacer have been described [80] . Unfortunately, because of their structural homology to choline, double positively charged chemotypes are correlated with substantial toxicity due to off-target activities [81] . The lead series described by Zech et al. [81] , on the other hand, relies heavily on basic amines found in methyldiazepanes to bind to the choline site. A primary hydrogen bond is added by the protonated 1' nitrogen. It is important to remember that depending on local conditions like pH, the methyldiazepane moiety may be transiently protonated or present as a free base. Furthermore, diazepane is used in a wide variety of licensed medications, mainly to boost solubility or ADME parameters. In most cases, this moiety does not pose a significant toxicity risk. Compound 68 induced apoptosis at low μM concentrations in the ChoKα expressing line MDA-MB-415.

From their synthesis to the molecular basis of their binding mode, a review in the field of ChoK inhibitors was published [82] . The first library of asymmetric bispyridinium derivatives was reported by Rubio-Ruiz et al. [82] . To investigate potential new binding modes to the enzyme. 4(4-Chloro-N-methylanilino)pyridinium, 1-benzyl-4-(dimethylamino)pyridinium, and 4-pyrrolidinopyridinium fragments were combined in these structures, with both positively charged moieties linked with variable linkers. The plasticity of the choline binding site, the discovery of new exploitable binding sites, and the allosteric properties of this enzyme are all emphasized as strategies for developing inhibitors and their selectivity on ChoKα over ChoKβ. Rubbini 

The implication of ChoKα in a diversity of human cancers is well established. Its lack of expression is lethal, implying that its function is essential [7] , and its inhibition leads to either cell arrest in non-tumorigenic cells, or cell death in tumor cells [2, 3, [60] [61] [62] [63] . Since ChoKs use choline and ATP as substrates of the enzymatic reaction, inhibition of ChoKα activity by direct blockage of the Cho or ATP-binding pockets could be a specific and effective strategy to block cancer cell proliferation. A number of ChoKIs have been designed to achieve this goal [82] .

However, this strategy has been questioned since V-11-0711, a potent inhibitor of ChoKα that affect only its catalytic activity, is not able to induce cell death in breast tumour cells. Therefore, a non-catalytic function of ChoKα has been suggested [84, 85] . In keeping with this, a balance between ChoKα and ChoKβ may be required for the appropriate regulation of cell proliferation and reduction of the protein levels of ChoKα may be required in some cell systems for induction of cell death [19] . Furthermore, a chaperone activity has been reported for ChoKα [86, 87] . If this is correct, effective ChoKαIs must be able to simultaneously reduce ChoKα enzymatic activity and intracellular protein levels.

The enzymatic activity of ChoKα follows a ping-pong mechanism with an intermediary phosphorylated conformation that can be used for its inhibition without affecting ATP and Cho binding by a steric interaction [88, 89] . In keeping with this, RSM932A/TCD717 has a unique mechanism of action, different from any other ChoKαI so far described. Its effect is not competitive with choline or ATP [88] since it does not bind directly in the choline or ATP pockets as do previously characterized ChoKαIs, but rather in a proximal but novel location near the surface of the enzyme [90] . This characteristic renders a rather unique mechanism of action that potentiates its specificity and potent antitumor activity [78] .

Inhibitors such as RSM-932A or specific siRNAs with a drastic reduction of both the levels of ChoKα and its product, phosphorylcholine, show a drastic and selective induction of cell death in a large variety of tumor cells. Additionally, it has been suggested that RSM932A induces a conformational change that expose the unfolded structure of the enzyme to endogenous proteases resulting is a drastic reduction of the ChoKα protein levels [62, 88] . Consistent with this interpretation, inhibition of ChoKα activity with no significant reduction of its protein levels may not be sufficient to trigger cell death in tumor cells [84, 85] . However, in glioblastoma cells, inhibition of ChoKα by V-11-0711 is sufficient to significantly reduce cell viability, invasiveness and clonogenicity. Furthermore, this antitumoral effect was related to inhibition of the expression of EMT associated genes mediated by ChoKα, and was synergistic with temozolomide [91] . Thus, the effect on cell viability by ChoKIs may be cell type dependent.

Several studies have looked into the implications of interfering with ChoK function, which may clarify how ChoK specific inhibitors (ChoKIs) and ChoK silencing with specific siRNA operate. ChoK inhibition through pharmacological or siRNA approaches cause a variety of effects such as loss of mitochondrial potential and cytochrome c release [58] , increased ceramide output [61] , ER stress [62] , unfold protein response (UPR) [62] , and ROS homeostasis via glutathione levels [92] . Similar effects on ER stress have been reported in Arabidopsis [93] .

Decreased mitochondria function by pharmacological inhibition of ChoKα has been associated with a reduction of citrate synthase expression and AMPK activation. These effects are also related to an increase in glucose and acetate uptake in an attempt to overcome the metabolic stress. The final outcome is the induction of cell death including apoptosis or necrosis with an exquisite specificity towards cancer cells [60] [61] [62] [63] 91] .

The implication of ChoKβ in cancer is still not resolved. Genetic evidence has clearly demonstrated a role of ChoKβ in muscular dystrophy [9] [10] [11] [12] [13] . Furthermore, ChoKβ inhibition by siRNA has no detrimental effect in cell viability, maybe because ChoKα can supplement its absence as a redundant metabolic enzyme [9] . However, we cannot exclude the possibility of a role of ChoKβ in the modulation of the activity of ChoKα in different tissues and therefore can still play a critical role in the onset and progression of tumours [18, 19] .

Drug resistance, one of the major burdens in anticancer research, has been also investigated in the ChoK pathway. In NSCLC-derived cells, resistance to ChoKαIs is overcome by overexpression of acid ceramidase (ASAH1) in keeping with the known mechanism of action of ChoKαIs of inducing increased levels of ceramides [59, 61] and allows the identification of tumours resistant to treatment with ChoKαIs. Acquisition of resistance to ChoKαIs has also being found in pancreatic [31] and breast cancer cells (Lacal et al., unpublished) . In B-Cell lymphomas, resistance to lysine deacetylase inhibitors (KDACI) is linked to increased ChoKα activity and can be overcome by treatment with ChoKαIs (48) . Similar effects have been reported in TRAIL-resistant colorectal [57] and ovarian tumours [58] where ChoKαIs can restore sensitivity to TRAIL.

Differences in the response of normal, non-tumorigenic and cancer cells have been unveiled. Thus, in breast, lung and colon-derived tumor cells, a drastic alteration of the levels of expression of proteins involved in the regulation of endoplasmic reticulum (ER) stress and the unfold protein response (UPR) takes place as a consequence of ChoKα inhibition [62] . A completely different scenario is observed in non-tumorigenic cells, where a transient and attenuated ER stress response is observed. These alterations in protein expression occur in parallel with a drastic reduction in cyclin D1, RB and E2F1α in cancer cells which is not observed in non-tumorigenic cells [62] . Thus, maintenance of the RB-E2F1α complex in non-tumorigenic cells after ChoKα inhibition induces arrest in G0/G1 but not cell death [62] . These results are consistent with many studies where a differential effect was observed in cancer versus non-tumorigenic cells [2, 3] . Additionally, in Arabinopsis, a similar role for the CEK1 enzyme has been proposed for the management of ER stress [92] .

Likewise, specific interference with ChoKα siRNA drives cancer cells to apoptotic death [2, 3, [51] [52] [53] 55] . By contrast the specific ChoKα inhibitor V-11-0711 (IC 50 = 20 nM), causes reversible growth arrest [84] , similar to what is observed in non-tumorigenic cells with other inhibitors [60, 61, 93] . These observations suggest that ChoKα plays a role in cancer cell survival that is outside the PC synthesis pathway [84] in keeping with abundant information that links ChoKα with both metabolic and signal transduction functions. These results are further supported by transcriptome analysis in cancer cells since inhibition of ChoKα induce alteration of expression levels of genes involved in cell cycle regulation, apoptosis, and nucleotide metabolism [94] [95] [96] [97] .

In addition to all the reported effects on cell metabolism, interference with ChoKα activity and levels has also an effect in signal transduction pathways. ChoKα has been shown to be phosphorylated by c-Src in breast cancer cells and as a consequence, it associates with the EGFR and is translocated to the plasma membrane [98] . Phosphorylation takes place at Y197 and Y333 and increases its activity by 1.40-to 1.68-fold when ChoKα is expressed along with c-Src or EGFR, respectively, and about 2.5-fold when co-overexpressed with both EGFR and c-Src [98] . Phosphorylation of ChoKα seems to correlate with a higher cell proliferation rate. Recently, this interaction has been mapped at the SH3 domain of c-Src and the poly-proline region N-terminal of ChoKα [99] . The relationship of ChoKα and EGFR, has also been reported in lung [100] and liver tumors [35] and has been associated with resistance to EGFR inhibitors [35] . In prostate cancer, ChoKα acts as a chaperone for the androgen receptor (AR), elucidates a feed-forward signalling loop that maintains ChoKα expression and as a consequence reinforces AR signaling activity. This chaperone function confers a growth advantage to cancer cells where ChoKα is overexpressed [89, 90] .

By contrast, ChoKβ activity is regulated by PKA at residues Ser 39 and Ser 40, a process that depends on cAMP levels [101] . Phosphorylation of ChoKβ increases its catalytic efficacy for choline and ATP but not to ethanolamine, and results in an increased sensitivity to HC-3 inhibition. This could be consistent with that phosphorylation of ChoKβ may be a mechanism to regulate PC and PE synthesis and that phosphorylation could modulate the balance of both isoenzymes as previously described [19] . Additionally, a differential phosphorylation by c-Src and PKA suggests that both isoforms could be implicated in carcinogenesis although by alternative mechanisms and in different organs.

Regulation of ChoKs is therefore similar to other eukaryotic protein kinases (ePKs) and follows phosphorylation patterns, in keeping with their proposed common ancestor and with an early eukaryotic origin. In fact, both ePKs and ChoKs have been suggested to have played an important role in early evolution of highly complex eukaryotic cells [102] .

ERK and AKT phosphorylation are reduced by either pharmacological inhibition or siRNA silencing of ChoKα in breast and T-cell lymphoma cancer cells [103] [104] [105] . Finally, ChoKα is regulated by PI3K and Ral-GDS, two direct effectors of the Ras oncoprotein [106] .

MYC and HIF1 have been involved in the regulation of ChoKα expression [37, 107, 108] , suggesting that overexpression of ChoKα results from the acidification typical of solid tumors. HIF1 is modulated in gliomas by 2-HG (2-hydroxyglutarate), the product of IDH1 (isocitrate dehydrogenase 1), resulting in a reduction in ChoK and EtnK activity [109] .

ChoKα has been involved in neuronal differentiation [110] , a process mediated by CCAAT/Enhancer binding Protein-β (C/EBPβ) through the CHKA promoter and the involvement of the ERK1/2 pathway and KDM2B (Lysine Demethylase 2B) as a repressor [111, 112] . In keeping with this observation, a relationship with Parkinson's disease (PD) has been recently suggested for ChoKα by modulation of Daf-16 location and control of the lipid content [113] . Finally, ChoKα interacts with the nonstructural protein 5A (NS5A) and phosphatidylinositol-4-kinase III (PI4KIII), modulating hepatitis C virus (HCV) replication through a mechanism that involves the endoplasmic reticulum [114, 115] . Inhibition of ChoKα has therefore a potential antiviral function.

ChoK dysfunction has recently been linked to a variety of other human diseases. ChoKα deficiency is lethal during early embryo development in mice and Arabidopsis [7, 8] , while ChoKβ is dispensable for embryo viability but required for proper forelimb development [9] [10] [11] [12] [13] . Differential expression patterns [116, 117] , mitochondrial activity [118] , and neuromuscular junctions [119] are all effects of ChoKβ deficiency in mice. Inactivating mutations in the CHKB gene have been linked to human muscular dystrophy and myopathy in several recent studies [11, [120] [121] [122] [123] [124] [125] [126] [127] [128] . Since a lack of ChoKβ activity causes muscular dystrophy, any intervention that restores ChoKβ activity can help to restore normal muscle development in this pathological situation.

Regulation of both CHKA and CHKB genes have been reported to follow distinct patterns. Thus, the ChoKβ is regulated along with the carnitine palmitoyltransferase 1B (CPT1B) gene and it is involved in the modulation of brown adipose tissue to generate heat [129] . Curiously, ChoKα is regulated in a robust circadian expression pattern in the liver and adrenal glands [130] . These findings reinforce the idea of differential physiological roles for both isoenzymes.

Searching for mechanisms that will allow to control ChoK functions, it is of interest to remark that two recent reports involve miRNA species in a network of regulatory processes to control ChoKα levels and function. It has been shown that miR-367-3p targets the 3'-UTR of the chka mRNA transcript with strong affinity in MCF7 breast cancer cells and, as a consequence, represses its expression [131] . In the lung cancer cell lines H1355 and A549, miR-1-3p regulates the EGFR/MAPK/ChoKα signaling pathway through modulation of the expression of FAM83A (Family with sequence similarity 83, member A) [100] a gene involved in the onset of lung adenocarcinoma [132] . These interesting findings open the possibility to explore alternative strategies for the control of ChoKα levels and activity as therapeutic approaches.

Roughly 15% of the world population is affected at some point in their lives by an illness caused by a protozoan parasite. In the developing world, this figure increases to 65%. The available therapies for many of these types of protozoal infections are limited, inadequate, and increasingly useless as resistant strains emerge. At the same time, market logic ensures that research and development into novel drug therapies for treating many of these parasites is limited. Therefore, research into novel drug targets such as choline kinase is of paramount importance [133] .

Plasmodium falciparum is a parasite which is the causative agent of malaria, which is transmitted by mosquitoes. Developing alternatives for the treatment of malaria is extremely critical: new drug-resistant strains of P. falciparum continuously appear. This includes strains resistant to artemisinin, the current frontline drug of choice in endemic regions [134] . Novel drug targets must be established and thoroughly researched, and drug discovery programs need to focus on discovering inhibitors for these novel target. One relatively novel drug target is choline kinase, an enzyme in the pathway leading to the production of Phosphatidylcholine (PC) and phosphatidylethanolamine (PE).

PC and PE are two essential and highly represented phospholipids in found in the membranes of P. falciparum (40% and 35% of total phospholipids, respectively) [135] . The essential nature of these phospholipids means that the pathways responsible for their synthesis can be disrupted to eliminate this parasite [136] . In P. falciparum, as in other eukaryotes, PC and PE are synthesized from choline (Cho) or ethanolamine (Etn), respectively, via the Kennedy pathway. The first enzyme in this pathway in P. falciparum is choline kinase (pfChoK), which functions both as a choline kinase and an ethanolamine kinase. Importantly, pf-ChoK is more highly expressed in growth phases of P. falciparum [137] . Inhibition of pf-ChoK affects the parasite's viability in in vitro and mouse models of malaria [88, 138] . Therefore, pfChoK is a natural target for inhibition.

The first pfChoKI, hexadecyltrimethylammonium bromid (HBA), was reported in 2007. HBA significantly reduces PCho synthesis (by 57% at 10 µM) and parasite growth (81% at 20 µM) and reduces parasitemia by 50% in mice with doses of 5 mg/kg [138] .

Some promising inhibitors have been discovered by the repurposing of hChoK inhibitors [88, 139] . This repurposing strategy is effective because the primary structure of the active site of pf-ChoK (Figure 4) , as well as the tertiary structure ( Figure 5) , are highly conserved with respect to hChoK [140] . For example, hChoK inhibitors MN58b and RSM-932A inhibit pfChoK in the micromolar range [88] . These inhibitors also prevent growth of P. falciparum at the low nanomolar range. In addition, they are effective against both drugsensitive and drug-resistant strains. MN58b and RSM-932 also prevent intraerythrocytic development, which affects parasite egress and invasion. RSM-932A has an uncompetitive mechanism with respect to choline and ATP while MN58b has a competitive mechanism with respect to both substrates. Bisquinolinium bromide salt derivatives were discovered via high-throughput compound screening. These inhibitors were found to have affect P. falciparum growth at IC50s < 1μM and had IC50s against pf-ChoK in the low μM [141] .

Leishmania infantum is the causative agent of Leishmaniasis, a disease found in the tropics and subtropics and transmitted by phlebotomine sand fly. Leishmaniasis affects nearly 1 million people a year [142] . There has been an initial study whether or not ChoK inhibition is an effective strategy for eliminating L, infantum parasites. A number of quaternary ammonium salts were screened, however the most effective inhibitor, (N-iodomethyl-N,N-dimethyl-N-(6,6-diphenylhex-5-en-1-yl) ammonium iodide, only inhibited liChoK in the mM range. Therefore, more work needs to be carried out to discover more powerful inhibitors against liChoK and to assess their ability to kill the parasite [143] . circle. The ATP binding site residues are marked with a red circle. While there is poor overall alignment of sequences, there is strong conservation in the areas of the ATP and choline binding site, as can be seen by the number of residues that are either conserved or semiconserved. 

Trypanosoma brucei is the causative agent of Human African Trypanosomiasis also known as Africn sleeping sickness. This parasite is transmitted by the Tsetse fly. Several hundred thousand sub-Saharan Africans are infected yearly with this parasite, leading to 10,000 deaths. This parasite produces a single choline kinase isoform (tbChoK) [144] . Conditional knockouts tbChoK has demonstrated that this enzyme is critical for cell growth [145] . The only available treatments are often toxic and drug resistant strains have emerged [146] . To date, we have identified a single drug discovery study which utilized fragment based screening against tbChoK to identify leads that were effective against T. brucei at IC50s in the low μM [145] . These results suggest that tbChoK is a promising drug target.

The emergence of bacterial pathogens that are resistant to current antibiotic therapies underlines the need for continuous research into alternative therapies and the discovery of novel drug targets. A promising strategy is to exploit information regarding drug targets in eukaryotic systems and explore their analogs in bacterial systems. This is an ideal, cost-effective, and efficient strategy, because the same therapies that were developed at considerable expense in eukaryotic systems can be repurposed as antibiotics and antimicrobials. Promising examples of this strategy are bacterial pathways of which ChoK is a part. These pathways are best characterized in the Gram-positive Streptococcus pneumoniae and Gram-negative Haemophilius influenzae. Repurposed hChoK inhibitors which selectively inhibited the ethanolamine kinase function of pf-ChoK were also discovered. These blocked the growth of parasites at IC 50 s in the low nanomolar range. These inhibitors also caused a concomitant drop in cellular PE, leading to cell death [139] .

Bisquinolinium bromide salt derivatives were discovered via high-throughput compound screening. These inhibitors were found to have affect P. falciparum growth at IC 50 s < 1µM and had IC 50 s against pf-ChoK in the low µM [141] .

Leishmania infantum is the causative agent of Leishmaniasis, a disease found in the tropics and subtropics and transmitted by phlebotomine sand fly. Leishmaniasis affects nearly 1 million people a year [142] . There has been an initial study whether or not ChoK inhibition is an effective strategy for eliminating L, infantum parasites. A number of quaternary ammonium salts were screened, however the most effective inhibitor, (Niodomethyl-N,N-dimethyl-N-(6,6-diphenylhex-5-en-1-yl) ammonium iodide, only inhibited liChoK in the mM range. Therefore, more work needs to be carried out to discover more powerful inhibitors against liChoK and to assess their ability to kill the parasite [143] .

Trypanosoma brucei is the causative agent of Human African Trypanosomiasis also known as Africn sleeping sickness. This parasite is transmitted by the Tsetse fly. Several hundred thousand sub-Saharan Africans are infected yearly with this parasite, leading to 10,000 deaths. This parasite produces a single choline kinase isoform (tbChoK) [144] . Conditional knockouts tbChoK has demonstrated that this enzyme is critical for cell growth [145] . The only available treatments are often toxic and drug resistant strains have emerged [146] . To date, we have identified a single drug discovery study which utilized fragment based screening against tbChoK to identify leads that were effective against T. brucei at IC 50 s in the low µM [145] . These results suggest that tbChoK is a promising drug target.

The emergence of bacterial pathogens that are resistant to current antibiotic therapies underlines the need for continuous research into alternative therapies and the discovery of novel drug targets. A promising strategy is to exploit information regarding drug targets in eukaryotic systems and explore their analogs in bacterial systems. This is an ideal, cost-effective, and efficient strategy, because the same therapies that were developed at considerable expense in eukaryotic systems can be repurposed as antibiotics and antimicrobials. Promising examples of this strategy are bacterial pathways of which ChoK is a part. These pathways are best characterized in the Gram-positive Streptococcus pneumoniae and Gram-negative Haemophilius influenzae.

Choline is an essential nutrient for S. pneumoniae [147] and the choline kinase of S. pneumoniae (sChoK) is an essential enzyme [148] . This pathogen meets its needs for this metabolite in part by scavenging choline molecules from host cells using the Pce phosphodiesterase [149] . As in eukaryotes, the ChoK of S. pneumoniae (sChoK) phosphorylates choline (Cho) into phosphorylcholine (PCho). However, the subsequent metabolic pathways diverge from the Kennedy pathway: PCho is utilized in the pathways responsible for the synthesis of bacterial teichoic acids ( Figure 6 ). 

Choline is an essential nutrient for S. pneumoniae [147] and the choline kinase of S. pneumoniae (sChoK) is an essential enzyme [148] . This pathogen meets its needs for this metabolite in part by scavenging choline molecules from host cells using the Pce phosphodiesterase [149] . As in eukaryotes, the ChoK of S. pneumoniae (sChoK) phosphorylates choline (Cho) into phosphorylcholine (PCho). However, the subsequent metabolic pathways diverge from the Kennedy pathway: PCho is utilized in the pathways responsible for the synthesis of bacterial teichoic acids ( Figure 6 ). Figure 6 . The sChok pathway and how it fits into the overall process of teichoic acid assembly. PCho molecules (purple) are transferred from CDP-Cho to the GalNac residues (green) of glycan precursors (RXN 3). The glycan precursors then assemble to form pre-teichoic acids (RXN 4), which become either LTA or CTA molecules depending on their final destination. LTA and CTA are embedded in the membrane or cell wall, respectively.

These are lipoteichoic acid (LTA) and cell wall teichoic acid (CTA) [150, 151] . The LicC cytidylyl transferase catalyzes the production of CDP-choline from PCho ( Figure 6 , RXN 2). The LicD1 and LicD2 PCho transferases transfer the PCho to the N-acetylgalactosamine (GalNac) residues found on pre-teichoic acid glycan precursors ( Figure 6, RXN 3 ) [152] .

The precursers are then assembled by an uncharacterized enzyme into variably sized pre-teichoic acid molecules, (Figure 6, RXN 4) [152] . Pre-teichoic acid transport across the cell membrane then mediated by the teichoic acid flippase TacF to become either LTA or CTA. LTA and CTA are chemically similar, with the difference that LTA is embedded in the cell membrane via a glycolipid lipid anchor through the action of the TacL ligase [153] , and CTA is covalently attached to the cell wall via peptidoglycan molecules through the action of LCP phosphotransferases [145] . Meanwhile LTA is a known virulence factor and the LTA synthesis pathway has demonstrated to be a source of drug targets [154] .

Significantly, LidD2 knockouts of S. pneumoniae diminish virulence due to limitations in the number of PCho containing teichoic acids found on the cell surface [155] . PCho decoration of the cell surface is required for normal cell-functioning because the Cho moiety anchors choline binding proteins (CBPs) such as murein hydrolases LytA and LytaAB, which are critical factors for cell division [156] . In addition, it anchors the choline binding protein A (CBPA) which is a determinant of virulence [157] . Many other CBPs are involved in colonization and even sepsis [158] . Therefore, the choline metabolic pathways, of which Figure 6 . The sChok pathway and how it fits into the overall process of teichoic acid assembly. PCho molecules (purple) are transferred from CDP-Cho to the GalNac residues (green) of glycan precursors (RXN 3). The glycan precursors then assemble to form pre-teichoic acids (RXN 4), which become either LTA or CTA molecules depending on their final destination. LTA and CTA are embedded in the membrane or cell wall, respectively.

These are lipoteichoic acid (LTA) and cell wall teichoic acid (CTA) [150, 151] . The LicC cytidylyl transferase catalyzes the production of CDP-choline from PCho ( Figure 6, RXN 2) . The LicD1 and LicD2 PCho transferases transfer the PCho to the N-acetylgalactosamine (GalNac) residues found on pre-teichoic acid glycan precursors ( Figure 6, RXN 3) [152] .

The precursers are then assembled by an uncharacterized enzyme into variably sized pre-teichoic acid molecules, (Figure 6, RXN 4) [152] . Pre-teichoic acid transport across the cell membrane then mediated by the teichoic acid flippase TacF to become either LTA or CTA. LTA and CTA are chemically similar, with the difference that LTA is embedded in the cell membrane via a glycolipid lipid anchor through the action of the TacL ligase [153] , and CTA is covalently attached to the cell wall via peptidoglycan molecules through the action of LCP phosphotransferases [145] . Meanwhile LTA is a known virulence factor and the LTA synthesis pathway has demonstrated to be a source of drug targets [154] .

Significantly, LidD2 knockouts of S. pneumoniae diminish virulence due to limitations in the number of PCho containing teichoic acids found on the cell surface [155] . PCho decoration of the cell surface is required for normal cell-functioning because the Cho moiety anchors choline binding proteins (CBPs) such as murein hydrolases LytA and LytaAB, which are critical factors for cell division [156] . In addition, it anchors the choline binding Pharmaceutics 2021, 13, 788 18 of 28 protein A (CBPA) which is a determinant of virulence [157] . Many other CBPs are involved in colonization and even sepsis [158] . Therefore, the choline metabolic pathways, of which sChoK is an integral part, play an important role in S. pneumoniae growth and invasion.

Disrupting the teichoic acid production pathway can also disrupt S. pneumonaie cell growth. Therefore, it follows that disruption of any element of this pathway, such as sChoK, is a promising therapeutic avenue to follow. In addition, the same tools used the to inhibit eukaryotic ChoKs are likely to be effective against sChoK, particularly those know to interact with the substrate binding sites. This is because the choline and ATP sites of sChoks are highly conserved with respect to eukaryotic isoforms (Figure 4 ) as are the tertiary structures.

With this rationale in mind, the well-known choline analog and competitive hChoKI was studied and found to inhibit sChoK and cell growth with a high IC 50 (in the mM range). This was also found to disrupt lipoteichoic acid production, which was consistent with the cell wall deformations that were observed by scanning electron microscopy. This established sChoK as a putative drug target [159] [160] [161] . Later, the hChoK inhibitors MN58b and RSM-932A were assayed and found to inhibit both sChoK activity and S. pneumoniae cell growth at moderate and low µM concentrations, to modulate lipoteichoic acid production and assembly, and to affect the cell wall. Interestingly, in the case of in vitro studies with purified sChoK, MN58 functions as a competitive inhibitor against both choline and ATP, while RSM932A functions as a competitive and non-competitive inhibitor against choline and ATP, respectively. This result against RSM932A is in sharp contrast to the observed uncompetitive behavior of this drug in the case of pf-ChoK. This indicates that there are likely enough differences in the largely conserved active sites of the different ChoKs to modulate ChoKI behavior. This promising outcome suggests that it will likely be possible to rationally design ChoKI that are effective but selective between the different isoforms.

The choline kinase of Haemophilius influenzae (hiChoK) is another promising target to explore for the design of inhibitors against this pathogen. Cho is not a nutritional requirement in the case of the Gram-negative H. influenzae, as it is in S. pneumoniae. Nevertheless, this pathogen does uptake this metabolite from its surroundings [162] , including from host cells [163] H. influenzae produces PCho and uses it to decorate its lipopolysaccharides (LPS). PCho containing LPS molecules mediate the interactions between the pathogen and host and help the pathogen avoid host immune responses [149] . By mimicking the phosphatidylcholine of eukaryotic host cells, these LPS molecules shield the pathogen from attack from host cell produced anti-microbial peptides [164] and antibodies [165] . It is important to note that the LicA gene that codes for hiChoK is upregulated during the colonization steps of H. influenzae [166] . PCho is also important for pathogenesis of H. influenzae, because this molecule mediates cell adhesion to the host PAF receptor [162] in a step which leads to invasion the respiratory tract [149] . In addition, PCho decoration of lipoteichoic acid has been shown to reduce the binding of a bacteriocidal antibodies [164] .

In animal models of rheumatoid arthritis (RA), ChoKIαs are also very successful, suggesting that these drugs have the potential to be used as potent therapeutics in inflammatory diseases [167] . Cell migration and resistance to apoptosis of cultured fibroblast-like synoviocytes (FLS), which are involved in cartilage destruction in RA, were suppressed by MN58b, a powerful specific ChoKαI. ChoKα inhibition with MN58b significantly decreased FLS migration and proliferation, and abrogated joint inflammation and damage in either pretreatment or proven disease protocols in the K/BxN arthritis mouse model [167] .

These findings are in keeping with studies that postulate that synovial inflammation, hyperplasia, and joint destruction are all hallmarks of RA [168] . Phosphoinositide 3-kinase (PI3K)/Akt and Mitogen-activated protein kinase (MAPK) are involved in the control of FLS activity in RA, including matrix metalloproteinases (MMP) expression, synoviocyte growth and survival, and are the subject of therapeutic intervention in RA [169] . As previously mentioned, MN58b selectively inhibits ChoKα, which inhibits MAPK and PI3K/Akt signalling [103] [104] [105] , while PI3K inhibition affects ChoK function [170] . Finally, disruption of ChoKα activity affects phosphorylation of Rb, downmodulates cyclin D1, and interferes with PDGF signaling in FLS, as previously demonstrated in other cell systems [62] .

Using the C. Elegans as a model for Parkinson's disease (PD), it has been suggested that ChoK may be an important player in PD since inhibition of its expression by siRNA significantly reduces the nuclear localization of Daf-16. ChoK silenced worms showed decreased lipid content, a parameter of immense importance in PD-associated endpoints [113] .

Furthermore, ChoKα inhibitors have been shown to be very active in the treatment of several animal models for human diseases related to dysregulation of the inflammasome [171] . Thus, in the LPS-induced septic shock model, ChoKα inhibition dramatically reduced LPS-induced death, an effect associated with reduced levels of circulating IL-1β. Additionally, ChoKα inhibition has therapeutic effects in three models of chronic syndromes associated with cryopyrin (CAPS): Muckle-Wells syndrome (MWS), familial cold autoinflammatory syndrome (FCAS) and neonatal-onset multisystem inflammatory disease (NOMID). These three syndromes are a consequence of mutations in the NLRP3 gene that cause chronic activation of the inflammasome [171] . Therefore, ChoKα inhibition may play an important role in the treatment of a diversity of human diseases related to an altered inflammatory response.

ChoKα is overexpressed in a large number of human tumors, and blocking its function causes cancer cells to die while non-tumorigenic cells undergo a reversible cell cycle arrest. Based on a novel mechanism of action, it makes possible combination of ChoK inhibitors with many current therapeutic approaches. Thus, specific targeting of this enzyme is a novel strategy for the treatment of many cancer types. Other diseases for which ChoKα inhibitors have been shown to be effective include malaria, rheumatoid arthritis, inflammation, parasites, and pathogenic bacteria.

One of our ChoKα inhibitors, RSM932A/TCD717, has reached the first in human Phase I clinical trial [79] . This first step towards the use of this novel family of drugs in the regular clinical practice has been a milestone that has paved the way for future development of successful drugs. Although further work is needed to finally reach the clinic as a standard of care in any of the several pathological conditions where ChoKIs have been proposed as a therapeutic strategy, the most important issues as the preclinical and the toxicology studies have been already addressed.

There is limited research into ChoKIs of parasites and therefore a limited number of promising ChoK drug leads. Likewise, much of this research has not yet reached the pre-clinical stage, nor therefore Phase I trials. Given this dearth in research, repurposing hChoK inhibitors may be good strategy for discovering novel treatments for parasites. As hChoKIs become cleared for use in humans to treat pathologies such as cancer, rheumatoid arthritis and inflammation, the immense amount of research required to reach that point can be exploited to find new treatments for illnesses such as malaria.

Despite the importance of hiChoK in pathogenesis of H. influenzae, the idea that hiChoK could function as a therapeutic target, remains untested. While, hiChoK has not yet been identified as an enzyme critical for cell growth, it is likely that inhibitors for this enzyme could be developed and employed as therapeutic adjuncts designed to assist the immune system in defending against infection.

Meanwhile, the improvements in sChoK inhibition observed with MN58b and RSM932A fully validated the idea that inhibitors of eukaryotic ChoKs could be repurposed for use against prokaryotes [172, 173] . However, sChoK still needs to be fully validated as the drug target responsible for these physiological effects and further metabalonomic and/or mutational studies need to be carried to fully establish this system.

In addition, more refined research needs to be carried out to fully distinguish the effects that these drugs might have on other choline binding proteins such as the autolysin LytA and many others [156] . The autolysin LytA mediates cell autolysis in S. pneumoniae, therefore, as an initial step, any putative sChoK inhibitor should also be assayed for its ability to modulate the autolytic process [171, 172] . For example, HC-3 is known to modulate autolysis [174] , but MN58b and RSM932A do not. Precision about the actual drug targets is necessary. However, more than one target for putative sChoK inhibitor would actually be highly beneficial. Multiple disruptions in more than one part of the choline metabolism or choline binding pathway may help prevent the development of new resistance to this novel family of inhibitors.

ChoK and other CBPs may be produced by other many bacterial species. Therefore, ChoKIs, like many other drugs [175] may affect species found in the human microbiome. Future studies will need to take these possible effects into account in determine the effects of ChoKIs on human health. The final goal should be to develop a ChoKI therapeutic that is specific to microbial choline kinases, and preferably targeted against specific pathogenic species. Preventing unintended consequences will require a more complete and rationalized understanding of the structural differences between the different ChoKs and CPBs of the various species.

Recently, a functional relationship between the immune checkpoint programmed cell death protein-1 (PD-1) and ChoKα has been established [176] . PD-L1 regulates metabolism through ChoKα, COX-2, and TGF-β, a new twist in the application of combinatorial therapies targeting both the immune checkpoints and modulation of critical enzymes involved in cancer metabolism. Thus, a new door has been opened to further investigate a potential relationship of ChoKα and the immune response.

Finally, since the mechanism of action of ChoKα inhibitors is based on the inhibition of the production of the ILs responsible for these inflammatory processes [166, 170] there is another potential application. ChoKαIs reduce macrophage activation and NLRP3 inflammasome attenuating the inflammatory response by modulating the production of IL-6, IL-1β and IL-18. All these processes are carried out by inhibiting ChoKα, the therapeutic target of our inhibitors. COVID-19 patients present in its most acute phases the Severe Acute Respiratory Syndrome (SARS), caused by strong bilateral lung inflammation, responsible for their death. This inflammatory process is associated with a strong increase in cytokine levels mediated by the inflammasome [177] process that coincides with studies carried out with SARS-Cov in which a relationship with the activation of the NLRP3 inflammasome was observed [178] . Based on these observations, it is reasonable to assume that inhibition of the phosphatidylcholine synthesis pathway through inhibition of the ChoKα enzyme could be an effective treatment in COVID-19 patients in the most advanced stages of the disease. This also deserves further investigation. 

Cellular metabolism and disease: What do metabolic outliers teach us? Cell

Choline kinase: A novel target to search for antitumoral drugs

Choline kinase as a precision medicine target for therapy in cancer, autoimmune diseases and malaria

Structure and function of choline kinase isoforms in mammalian cells

Phosphatidylcholine and the CDP-choline cycle

Molecular structure and differential function of choline kinases CHKalpha and CHKbeta in musculoskeletal system and cancer

Early Embryonic Lethality Caused by Disruption of the Gene for Choline Kinase α, the First Enzyme in Phosphatidylcholine Biosynthesis

The Choline/Ethanolamine Kinase Family in Arabidopsis: Essential Role of CEK4 in Phospholipid Biosynthesis and Embryo Development

A Rostrocaudal Muscular Dystrophy Caused by a Defect in Choline Kinase Beta, the First Enzyme in Phosphatidylcholine Biosynthesis

Functional rescue in a mouse model of congenital muscular dystrophy with megaconial myopathy

Choline Kinase Mutant Mice Exhibit Reduced Phosphocholine, Elevated Osteoclast Activity, and Low Bone Mass

Phosphatidylcholine metabolism and choline kinase in human osteoblasts

Megaconial congenital muscular dystrophy due to loss-of-function mutations in choline kinase β

The Four Arabidopsis Choline/Ethanolamine Kinase Isozymes Play Distinct Roles in Metabolism and Development

Differential role of choline kinase alpha and beta isoforms in human carcinogenesis

Elucidation of Human Choline Kinase Crystal Structures in Complex with the Products ADP or Phosphocholine

Crystal Structures of Human Choline Kinase Isoforms in Complex with Hemicholinium-3: Single amino acid near the active site influences inhibitor sensitivity

Expression and characterization of the active molecular forms of choline/ethanolamine kinase-alpha and -beta in mouse tissues, including carbon tetrachloride-induced liver

Balance of human choline kinase isoforms is critical for cell cycle regulation

Increased choline kinase activity in human breast carcinomas: Clinical evidence for a potential novel antitumoral strategy

Overexpression of choline kinase is a frequent feature in human tumor-derived cell lines and in lung, prostate and colorectal human cancers

Expression of choline kinase alfa to predict outcome in patients with early-stage non-small-cell lung cancer: A retrospective study

CHKA mediates the poor prognosis of lung adenocarcinoma and acts as a prognostic indicator

Overexpression of CHKA contributes to tumor progression and metastasis and predicts poor prognosis in colorectal carcinoma

A critical role for choline kinase-α in the aggressiveness of bladder carcinomas

Exploiting altered patterns of choline kinase-alpha expression on human prostate tissue to prognosticate prostate cancer

Comparative Metabolomic and Lipidomic Analysis of Phenotype Stratified Prostate Cells

Metabolic Reprogramming by Malat1 Depletion in Prostate Cancer (Stage 2)

Alterations of Choline Phospholipid Metabolism in Ovarian Tumor Progression

Alterations of Choline Phospholipid Metabolism in Endometrial Cancer Are Caused by Choline Kinase Alpha Overexpression and a Hyperactivated Deacylation Pathway

Choline Kinase Alpha (CHKα) as a Therapeutic Target in Pancreatic Ductal Adenocarcinoma: Expression, Predictive Value, and Sensitivity to Inhibitors

A genetic database can be utilized to identify potential biomarkers for biphenotypic hepatocellular carcinoma-cholangiocarcinoma

Epigenetic footprint enables molecular risk stratification of hepatoblastoma with clinical implications

Choline Kinase Alpha and Hexokinase-2 Protein Expression in Hepatocellular Carcinoma: Association with Survival

Choline Kinase α Mediates Interactions Between the Epidermal Growth Factor Receptor and Mechanistic Target of Rapamycin Complex 2 in Hepatocellular Carcinoma Cells to Promote Drug Resistance and Xenograft Tumor Progression

Activation of choline kinase drives aberrant choline metabolism in esophageal squamous cell carcinomas

Dysregulated choline metabolism in T-cell lymphoma: Role of choline kinase-α and therapeutic targeting

Vitro Hepatitis C Virus Infection and Hepatic Choline Metabolism. Viruses

The metabolic responses to hepatitis B virus infection shed new light on pathogenesis and targets for treatment

Expression profiling of choline and ethanolamine kinases in MCF7, HCT116 and HepG2 cells, and the transcriptional regulation by epigenetic modification

Magnetic Resonance Spectroscopy for Detection of Choline Kinase Inhibition in the Treatment of Brain Tumors

Knockdown of choline kinase α (CHKA) inhibits the proliferation, invasion and migration of human U87MG glioma cells

Metabolic Imaging of Pancreatic Ductal Adenocarcinoma Detects Altered Choline Metabolism

EB-3D a novel choline kinase inhibitor induces deregulation of the AMPK-mTOR pathway and apoptosis in leukemia T-cells

Choline kinase activation is a critical requirement for the proliferation of primary human mammary epithelial cells and breast tumor progression

Choline kinase overexpression increases invasiveness and drug resistance of human breast cancer cells

Elevated Choline Kinase α-Mediated Choline Metabolism Supports the Prolonged Survival of TRAF3-Deficient B Lymphocytes

Metabolomic Profiling Reveals Cellular Reprogramming of B-Cell Lymphoma by a Lysine Deacetylase Inhibitor through the Choline Pathway

Phospholipase D1 and choline kinase-α are interactive targets in breast cancer

Downregulation of Choline Kinase-Alpha Enhances Autophagy in Tamoxifen-Resistant Breast Cancer Cells

RNA Interference-Mediated Choline Kinase Suppression in Breast Cancer Cells Induces Differentiation and Reduces Proliferation

Choline kinase alpha depletion selectively kills tumoral cells

Noninvasive detection of lentiviralmediated choline kinase targeting in a human breast cancer xenograft

Combined 5-FU and ChoKα inhibitors as a new alternative therapy of colorectal cancer: Evidence in human tumor-derived cell lines and mouse xenographs

Choline Kinase Down-regulation Increases the Effect of 5-Fluorouracil in Breast Cancer Cells

Choline Kinase Alpha Inhibition by EB-3D Triggers Cellular Senescence, Reduces Tumor Growth and Metastatic Dissemination in Breast Cancer

Choline kinase inhibitors synergize with TRAIL in the treatment of colorectal tumors and overcomes TRAIL resistance

Choline kinase alpha impairment overcomes TRAIL resistance in ovarian cancer cells

Acid ceramidase as a chemotherapeutic target to overcome resistance to the antitumoral effect of choline kinase α inhibition

Inhibition of choline kinase renders a highly selective cytotoxic effect in tumor cells through a mitochondrial independent mechanism

Choline kinase inhibition induces the increase in ceramides resulting in a highly specific and selective cytotoxic antitumoral strategy as a potential mechanism of action

Endoplasmic reticulum stress participates in the cytotoxic effect of choline kinase α inhibitors in tumor cells

Near infrared fluorescent imaging of choline kinase alpha expression and inhibition in breast tumors

QSAR of 1,1 -(1,2-ethylenebisbenzyl)bis(4-substitutedpyridinium) dibromides as choline kinase inhibitors: A different approach for antiproliferative drug design

Structure-activity aspects of hemicholinium-3 (HC-3) and its analogs and congeners

Symmetrical Bis-Quinolinium Compounds: New Human Choline Kinase Inhibitors with Antiproliferative Activity against the HT-29 Cell Line

Atomic physicochemical parameters for three dimensional structure directed quantitative structure-activity relationships. 4. Additional parameters for hydrophobic and dispersive interactions and their application for an automated superposition of certain naturally occurring nucleoside antibiotics

Pallas Frame Module, a Prediction Tool of Physicochemical Parameters

Electrical Effect Substituent Constants for Correlation Analysis

Substituent Constant for Correlation Analysis in Chemistry and Biology

Quantitative structure-activity relationships for a series of symmetrical bisquaternary anticancer compounds

Anticancer bisquaternary heterocyclic compounds: A ras-ional design. Il Farm

QSAR studies on enzyme inhibitors

Molecular recognition in aqueous media. New binding studies provide further insights into the cation-.pi. interaction and related phenomena

Hansch Analysis and Related Approaches

Quantitative Drug Design

Chemische Struktur und Biologische Aktivität von Wirkstoffen

Preclinical Characterization of RSM-932A, a Novel Anticancer Drug Targeting the Human Choline Kinase Alpha, an Enzyme Involved in Increased Lipid Metabolism of Cancer Cells

Study of Intravenous TCD-717 in Patients with Advanced Solid Tumours. ClinicalTrials.gov Identifier: NCT01215864. Available online

Design, synthesis, crystallization and biological evaluation of new symmetrical biscationic compounds as selective inhibitors of human Choline Kinase α1 (ChoKα1)

Novel Small Molecule Inhibitors of Choline Kinase Identified by Fragment-Based Drug Discovery

Recent advances in the design of choline kinase α inhibitors and the molecular basis of their inhibition

Lead optimization-hit expansion of new asymmetrical pyridinium/quinolinium compounds as choline kinase α1 inhibitors

A non-catalytic role of choline kinase alpha is important in promoting cancer cell survival

Choline kinase-α protein and phosphatidylcholine but not phosphocholine are required for breast cancer cell survival

Choline Kinase Alpha as an Androgen Receptor Chaperone and Prostate Cancer Therapeutic Target

Kinase joins the chaperone club: Androgen-regulated kinome reveals choline kinase alpha as a potential drug target in prostate cancer

Antiplasmodial Activity and Mechanism of Action of RSM-932A, a Promising Synergistic Inhibitor of Plasmodium falciparum Choline Kinase

Kinetic and mechanistic characterisation of Choline Kinase-α

Identification of a Unique Inhibitor-Binding Site on

Reciprocal regulation of the cholinic phenotype and epithelial-mesenchymal transition in glioblastoma cells

Global metabolic profile identifies choline kinase alpha as a key regulator of glutathione-dependent antioxidant cell defense in ovarian carcinoma

Arabidopsis CHOLINE/ETHANOLAMINE KINASE 1 (CEK1) is a primary choline ekinase localized at the endoplasmic reticulum (ER) and involved in ER stress tolerance

The novel choline kinase inhibitor ICL-CCIC-0019 reprograms cellular metabolism and inhibits cancer cell growth

Choline kinase as a link connecting phospholipid metabolism and cell cycle regulation: Implications in cancer therapy

Disctinct choline metabolic profiles are associated with differences in gene expression for basal-like and luminallike breast cancer xenograft models

Interplay of choline metabolites and genes in patient-derived breast cancer xenografts

Functional interactions between choline kinase-α, epidermal growth factor receptor and c-Src in breast cancer cell proliferation

Molecular basis for the interaction between human choline kinase alpha and the SH3 domain of the c-Src tyrosine kinase

Involvement of MicroRNA-1-FAM83A Axis Dysfunction in the Growth and Motility of Lung Cancer Cells

Phosphorylation of Human Choline Kinase Beta by Protein Kinase A: Its Impact on Activity and Inhibition

Evolutionary Ancestry of Eukaryotic Protein Kinases and Choline Kinases

Regulation of Akt(ser473) phosphorylation by Choline kinase in breast carcinoma cells

Selective inhibition of choline kinase simultaneously attenuates MAPK and PI3K/AKT signaling

A novel small molecule antagonist of choline kinase-α that simultaneously suppresses MAPK and PI3K/AKT signaling

Regulation of Choline kinase activity by Ras proteins involves Ral-GDS and PI3K

Hypoxia regulates choline kinase expression through hypoxia-inducible factor-1 alpha signaling in a human prostate cancer model

Choline phosphorylation and regulation of transcription of choline kinase α in hypoxia

Mutant IDH1 gliomas downregulate phosphocholine and phosphoethanolamine synthesis in a 2-hydroxyglutarate-dependent manner

Phosphatidylcholine Biosynthesis during Neuronal Differentiation and Its Role in Cell Fate Determination

Choline kinase alpha expression during RA-induced neuronal differentiation: Role of C/EBPβ

KDM2B regulates choline kinase expression and neuronal differentiation of neuroblastoma cells

A Systematic RNAi Screen of Neuroprotective Genes Identifies Novel Modulators of Alpha-Synuclein-Associated Effects in Transgenic Caenorhabditis elegans

Hepatitis C virus subverts human choline kinase-alpha to bridge phosphatidylinositol-4-kinase IIIalpha (PI4KIIIalpha) and NS5A and upregulates PI4KIIIalpha activation, thereby promoting the translocation of the ternary complex to the endoplasmic reticulum for viral replication

Human Choline Kinase-α Promotes Hepatitis C Virus RNA Replication through Modulation of Membranous Viral Replication Complex Formation

Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse

Understanding the muscular dystrophy caused by deletion of choline kinase beta in mice

Muscle choline kinase beta defect causes mitochondrial dysfunction and increased mitophagy

Myotonic Dystrophy Transgenic Mice Exhibit Pathologic Abnormalities in Diaphragm Neuromuscular Junctions and Phrenic Nerves

A congenital muscular dystrophy with mitochondrial structural abnormalities caused by defective de novo phosphatidylcholine biosynthesis

Sequential neuroradiological and neurophysiological studies in a Japanese girl with merosin-deficient congenital muscular dystrophy

Congenital megaconial myopathy due to a novel defect in the choline kinase Beta gene

Muscular dystrophy with large mitochondria associated with mutations in the CHKB gene in three British patients: Extending the clinical and patho-logical phenotype

Exome sequencing identifies a CHKB mutation in Spanish patient with megaconial congenital muscular dystrophy and mtDNA depletion

Novel CHKB mutation expands the megaconial muscular dystrophy phenotype

New splicing mutation in the choline kinase beta (CHKB) gene causing a muscular dystrophy detected by whole-exome sequencing

Congenital neurogenic muscular atrophy in megaconial myopathy due to a mutation in CHKB gene

Clinical characteristics of megaconial congenital muscular dystrophy due to choline kinase beta gene defects in a series of 15 patients

Emergent Coordination of the CHKB and CPT1B Genes in Eutherian Mammals: Implications for the Origin of Brown Adipose Tissue

The hepatic circadian clock regulates the choline kinase a gene through the BMAL1-REV-ERBα axis

MicroRNA 367 3p induces apoptosis and suppresses migration of MCF 7 cells by downregulating the expression of human choline kinase α

FAM83A and FAM83A-AS1 both play oncogenic roles in lung adenocarcinoma

Available online

Artemisinin Resistance inPlasmodium falciparumMalaria

A pathway for phosphatidylcholine biosynthesis in Plasmodium falciparum involving phosphoethanolamine methylation

Targeting the lipid metabolic pathways for the treatment of malaria

Molecular characterization and localization of Plasmodium falciparum choline kinase

Inhibition of Plasmodium falciparum Choline Kinase by Hexadecyltrimethylammonium Bromide: A Possible Antimalarial Mechanism

Plasmodium falciparum Choline Kinase Inhibition Leads to a Major Decrease in Phosphatidylethanolamine Causing Parasite Death

Crystal Structure of the Apo and the ADP-Bound Form of Choline Kinase from Plasmodium falciparum

Development of a new and reliable assay for choline kinase using (31)P NMR. Heliyon

Identification of inhibitors for putative malaria drug targets among novel antimalarial compounds

Biochemical characterization of the initial steps of the Kennedy pathway in Trypanosoma brucei: The ethanolamine and choline kinases

Coupled Enzyme Activity and Thermal Shift Screening of the Maybridge Rule of 3 Fragment Library against Trypanosoma brucei Choline Kinase; A Genetically Validated Drug Target

Drugs and drug resistance in African trypanosomiasis. Drug Resist. Updates

Choline in the Cell Wall of a Bacterium: Novel Type of Polymer-Linked Choline in Pneumococcus

Drastic reduction in the virulence of Streptococcus pneumoniae expressing type 2 capsular polysaccharide but lacking choline residues in the cell wall

Review of phosphocholine substituents on bacterial pathogen glycans: Synthesis, structures and interactions with host proteins

Incorporation of choline into Streptococcus pneumoniae cell wall antigens: Evidence for choline kinase activity

Synthesis of glycerol phosphate lipoteichoic acid in Staphylococcus aureus

Biosynthesis of teichoic acids in Streptococcus pneumoniae and closely related species: Lessons from genomes

Lipoteichoic acid deficiency permits normal growth but impairs virulence of Streptococcus pneumoniae

Role of lipoteichoic acid in infection and inflammation

Pneumococcal licD2 gene is involved in phosphorylcholine metabolism

Choline Binding Proteins from Streptococcus pneumoniae: A Dual Role as Enzybiotics and Targets for the Design of

Contribution of novel choline-binding proteins to adherence, colonization and immunogenicity of Streptococcus pneumoniae

Role of Novel Choline Binding Proteins in Virulence of Streptococcus pneumoniae

A dual choline/phosphocholine colorimetric method for measuring the relative strength of inhibitors of choline kinases of Gram-positive pathogens

A Novel Drug Target for the Inhibition of Streptococcus pneumoniae

Parallel Colorimetric Quantification of Choline and Phosphocholine as a Method for Studying Choline Kinase Activity in Complex Mixtures

Decoration of lipopolysaccharide with phosphorylcholine: A phase-variable characteristic of Haemophilus influenzae

The transfer of choline from the host to the bacterial cell surface requires glpQ in Haemophilus influenzae

Bacterial Phosphorylcholine Decreases Susceptibility to the Antimicrobial Peptide LL-37/hCAP18 Expressed in the Upper Respiratory Tract

Phosphorylcholine Allows for Evasion of Bactericidal Antibody by Haemophilus influenzae

Choline kinase inhibition in rheumatoid arthritis

Evolving concepts of rheumatoid arthritis

Small-molecule therapeutics in rheumatoid arthritis: Scientific rationale, efficacy and safety

The novel phosphatidylinositide 3-kinase inhibitor PI103 downregulates choline kinase resulting in phosphocholine depletion detected by Magnetic Resonance Spectroscopy

Choline uptake and metabolism modulate macrophage IL-1β production

Identification and validation of novel and more effective choline kinase inhibitors against Streptococcus pneumoniae

Choline Kinase Emerges as a Promising Drug Target in Gram-Positive Bacteria

Autolyse the cell in order to save it? Inducing, then blocking, autolysis as a strategy for delaying cell death in the probiotic Lactobacillus reuteri

Effects of Long Term Exposure to Aspirin on Growth, Functionality and Protein Profile of Lactobacillus rhamnosus (LGG) (ATCC 53103)

The PD-L1 metabolic interactome intersects with choline metabolism and inflammation

Clinical features of patients infected with novel coronavirus in

Severe acute respiratory syndrome Coronavirus ORF3a protein activates the NLRP3 inflammasome by promoting TRAF3-dependent ubiquitination of ASC