key: cord-0984764-uhxol85c authors: Truffot, Aurélie; Andréani, Julien; Le Maréchal, Marion; Caporossi, Alban; Epaulard, Olivier; Germi, Raphaele; Poignard, Pascal; Larrat, Sylvie title: SARS-CoV-2 Variants in Immunocompromised Patient Given Antibody Monotherapy date: 2021-10-03 journal: Emerg Infect Dis DOI: 10.3201/eid2710.211509 sha: 353a4f92b813d6c7468eabe23247af77ef6c957d doc_id: 984764 cord_uid: uhxol85c A 72-year-old immunocompromised man infected with severe acute respiratory syndrome coronavirus 2 received bamlanivimab monotherapy. Viral evolution was monitored in nasopharyngeal and blood samples by melting curve analysis of single-nucleotide polymorphisms and whole-genome sequencing. Rapid emergence of spike receptor binding domain mutations was found, associated with a compartmentalization of viral populations. A 72-year-old immunocompromised man in France who had chronic lymphocytic leukemia associated with hypogammaglobinemia for 4 years experienced diarrhea, asthenia, fever, and cough associated with coronavirus disease (COVID-19). Although he had received 1 injection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mRNA vaccine (BNT162b2; Pfizer/BioNTech, https://www.pfizer.com) 20 days earlier, we confirmed a diagnosis of COVID-19 by using a semiquantitative SARS-CoV-2 reverse transcription PCR (RT-PCR) viral load assay. This assay showed a cycle threshold (C t ) value of 27 for a nasopharyngeal swab specimen. His most recent monoclonal antibody (mAb) chemotherapy treatment (venetoclax and rituximab) had been conducted 17 days earlier. Because of his immunocompromised status, treatment with bamlanivimab (LY-CoV555), a neutralizing IgG1 mAb, was initiated at day 0, 4 days after onset of symptoms (Table) . The patient received an infusion of 700 mg in a single dose and was discharged. Analysis of samples showed a high viral load in a nasopharyngeal swab specimen (C t 20) and a blood sample (C t 37) (Table) . Three days after the mAb infusion, the patient's symptoms worsened, and he was hospitalized in the Infectious Diseases Department at Grenoble Hospital (Grenoble, France) on day 6. The condition of the patient had deteriorated; he had an additional need for oxygen, which resulted in a convalescent-phase plasma transfusion on day 10. After this treatment, the condition of the patient continued to deteriorate, and he was transferred to the intensive care unit on day 13. A high dose of corticosteroids was given on days 21-26. This treatment resulted in an improvement of his respiratory condition, but the patient remained dependent on supplemental oxygen (6 L/min). The patient was discharged from the intensive care unit and returned 2726 Emerging Infectious Diseases • www.cdc.gov/eid • Vol. 27, No. 10, October 2021 to the infectious disease department on day 33, but still had a high viral load in nasopharyngeal swab specimens (C t v20 on day 45). Because of this persistent viral replication, the patient was given remdesivir on day 47 and this treatment was continued for 10 days (200 mg for 1 day, followed by 100 mg/d for 9 days). SARS-CoV-2 carriage in a nasopharyngeal swab specimen decreased during treatment, and the patient was discharged from the infectious disease department and transferred to a rehabilitation center. The nasopharyngeal swab specimen viral load became negative on day 61. To monitor viral evolution, we performed a multiplex RT-PCR based on melting curve analyses with VirSNIP Kits (TIB Molbiol, https://www.tib-molbiol. de) to evaluate the presence of the S: E484K and S: N501Y mutations in SARS-CoV-2 variants. Three days after mAb treatment (day 3), RT-PCR results suggested the presence of S: N501Y and an absence of S:E484K on an nasopharyngeal swab specimen. On day 6, the S:N501Y mutation was still present but was also found associated with an undetermined mutation at position 484 (melting temperatures different from those of wild-type E and the mutated strain K). On day 11, we detected the S: N501Y mutation in a blood sample but found no mutation at position 484. No nasopharyngeal swab specimen or blood sample from before mAb administration was available for analysis and comparison. We performed whole-genome sequencing on 12 clinical samples by using amplicon-based technology on the Ion Torrent Platform (ThermoFisher, https:// www.thermofisher.com) according to the protocol of and plug-ins used by Sjaarda et al. (1) . We confirmed results of this analysis by using the minimap2 program (2) . This analysis detected clade 20I/501Y.V1, Alpha variant (Pangolin: B.1.1.7), on day 3 in nasopharyngeal swab specimens. Three days later (day 6), a novel mutation (G23012C, S: E484Q) appeared in nasopharyngeal swab specimens at frequency of 82%, which rapidly reached >99% (S: E484Q) 10 days after mAb treatment (Table; Figure) . Eleven days after the mAb infusion, we detected an additional nucleotide mutation A23040G (S: Q493R) in only a blood sample at a frequency of 64%. This rate reached 76% at day 17 without any detection in nasopharyngeal swab specimens. Clinical trials of monotherapy treatment for SARS-CoV-2 infection have shown that subsequent dynamic shifts in the viral population appear to be frequent (3, 4) . An in vitro model showed that E484 and Q493 are 2 amino acid mutations of the spike protein that are known to be critical for bamlanivimab binding (5, 6) . The S: E484Q mutation is a hotspot of escape and could reduce susceptibility to bamlanivimab by >1,000-fold (6) and S: Q493R by >6,666-fold (7) . Use of bitherapy with bamlanivimab and etesevimab decreases the risk for emergence of drug-resistant variants (5, 8) . However, an escape mutation after use of this drug combination was recently described (7) . Our analysis identified signs of compartmentalized viral populations on the basis of sequences recovered in blood and nasopharyngeal swab samples (notably on day 17). Such a phenomenon has been reported in clinical trials (9, 10) . Further analysis is needed to distinguish genetic changes that occur in the primary viral population from apparent changes to clarify whether such escape mutants are enough to spread and persist in humans and how SARS-CoV-2 displays compartmentalized replication. Genomic surveillance for SARS-CoV-2 variants is encouraged for COVID-19 patients given mAbs as monotherapy or biotherapy. Dr. Truffot is a physician in the Department of Virology, University Hospital of Grenoble, Grenoble, France. Her research interests include novel sequencing technologies for genome diagnosis and follow-up of major pathogens, including SARS-CoV-2, and quantification of monoclonal antibodies by high-performance liquid chromatography/ mass spectrometry. World Organisation for Animal Health. SARS-COV-2 in animals-situation report 1 Detection of canine distemper virus nucleoprotein RNA by reverse transcription-PCR using serum, whole blood, and cerebrospinal fluid from dogs with distemper disease and diplomacy: GISAID's innovative contribution to global health Search and clustering orders of magnitude faster than BLAST MAFFT multiple sequence alignment software version 7: improvements in performance and usability RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies From People to Panthera: natural SARS-CoV-2 infection in tigers and lions at the Bronx Zoo Phylogenomics reveals viral sources, transmission, and potential superinfection in early-stage COVID-19 patients in Ontario Minimap2: pairwise alignment for nucleotide sequences Effect of bamlanivimab as monotherapy or in combination with etesevimab on viral load in patients with mild to moderate COVID-19: a randomized clinical trial Bamlanivimab treatment leads to rapid selection of immune escape variant carrying E484K mutation in a B.1.1.7 infected and immunocompromised patient Antibody cocktail to SARS-CoV-2 spike protein prevents rapid mutational escape seen with individual antibodies Complete map of SARS-CoV-2 RBD mutations that escape the monoclonal antibody LY-CoV555 and its cocktail with LY-CoV016 Emergence of SARS-CoV-2 spike protein escape mutation Q493R after treatment for COVID-19 The monoclonal antibody combination REGEN-COV protects against SARS-CoV-2 mutational escape in preclinical and human studies Evolution of viral quasispecies during SARS-CoV-2 infection Compartmentalized replication of SARS-Cov-2 in upper vs. lower respiratory tract assessed by whole genome quasispecies analysis. Microorganisms V ariants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) usually result from random mutations in humans or other hosts, but accelerated evolution can also occur under selective pressure from therapeutic interventions using