key: cord-0980406-ybqjv7co
authors: Zhang, Yuanyuan; Wang, Mingjie; Zhang, Xian; Liu, Tianxiao; Libby, Peter; Shi, Guo-Ping
title: COVID-19, the Pandemic of the Century and Its Impact on Cardiovascular Diseases
date: 2021-11-22
journal: Cardiol Discov
DOI: 10.1097/cd9.0000000000000038
sha: 7551f161011177a1d628e9b78f5e301197e4dd40
doc_id: 980406
cord_uid: ybqjv7co

COVID-19 caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection likely ranks among the deadliest diseases in human history. As with other coronaviruses, SARS-CoV-2 infection damages not only the lungs but also the heart and many other organs that express angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV-2. COVID-19 has upended lives worldwide. Dietary behaviors have been altered such that they favor metabolic and cardiovascular complications, while patients have avoided hospital visits because of limited resources and the fear of infection, thereby increasing out-hospital mortality due to delayed diagnosis and treatment. Clinical observations show that sex, age, and race all influence the risk for SARS-CoV-2 infection, as do hypertension, obesity, and pre-existing cardiovascular conditions. Many hospitalized COVID-19 patients suffer cardiac injury, acute coronary syndromes, or cardiac arrhythmia. SARS-CoV-2 infection may lead to cardiomyocyte apoptosis and necrosis, endothelial cell damage and dysfunction, oxidative stress and reactive oxygen species production, vasoconstriction, fibrotic and thrombotic protein expression, vascular permeability and microvascular dysfunction, heart inflammatory cell accumulation and activation, and a cytokine storm. Current data indicate that COVID-19 patients with cardiovascular diseases should not discontinue many existing cardiovascular therapies such as ACE inhibitors, angiotensin receptor blockers, steroids, aspirin, statins, and PCSK9 inhibitors. This review aims to furnish a framework relating to COVID-19 and cardiovascular pathophysiology.

The World Health Organization (WHO) has estimated that, since late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has infected approximately 220 million people worldwide, resulting in over 4.5 million deaths. Although COVID-19 was initially defined as an infectious respiratory disease, it is now known that this disease can affect organs other than the lungs, including the kidneys, liver, gastrointestinal tract, central nervous system, and cardiovascular system. [1] [2] [3] Early studies showed that patients infected with SARS-CoV or Middle East respiratory syndrome coronavirus (MERS-CoV) developed severe cardiovascular complications. SARS-CoV infection occurred mostly in patients with hyperlipidemia, cardiovascular disorders, and abnormalities of glucose metabolism, [4, 5] whereas MERS-CoV infection was found preferentially in patients with underlying cardiovascular disease (CVD), such as congestive heart failure (HF). [6, 7] The outbreak of COVID-19 has changed our lifestyle. An online survey from Europe, North America (including the US), and western Asia indicated that reduced activity due to COVID-19-related restrictions led to unhealthier food choices. [8] Social isolation changed dietary behaviors. [9] Adolescents were less physically active and showed increased consumption of ultra-processed foods, [10] leading to other public health issues [ Figure 1 ]. Indeed, obese Italian individuals gained an average of 1.5 kg in weight after 1 month of strict lockdown, [11] thereby increasing their risk of CVD. Here, we offer an overview of our current understanding of the cardiovascular consequences of COVID-19. Patients with CVD may have heightened susceptibility to SARS-CoV-2, and, vice versa, COVID-19 may precipitate cardiovascular complications.

Besides advanced age, being male and having underlying medical conditions that are major risk factors for COVID-19associated mortality, such as cardiovascular complications (myocardial injury, myocarditis, acute coronary syndrome (ACS), cardiac hypertrophy, cardiac arrhythmia, HF, and even venous thromboembolic (VTE) disease) increased the mortality rate by twice as much as other risk factors. [12] Risk factors for CVD such as hypertension, obesity, and diabetes comorbidities are frequently found in COVID-19 patients and are associated with a high mortality rate. CVD and associated risks in COVID-19 patients are tightly linked with hospitalization, mechanical ventilation, admittance to intensive care, and in-and out-hospital deaths. The prevalence of hypertension and CVD in COVID-19 patients reportedly reaches 30% and 14.5%, respectively [13, 14] ; rates that increase in patients requiring mechanical ventilation or intensive care, and remain 2-fold higher in those who succumb to SARS-CoV-2. [6, 13, 15] Cardiac injury, as defined by raised troponin levels, occurs more frequently in COVID-19 patients who do not survive (>50%) than in those who survive (1%). Similarly, in a study of 197 hospitalized patients, cases of underlying diabetes (31% vs. 14%), hypertension (48% vs. 23%), and CVD (24% vs. 1%) were found to be higher in non-survivors than in survivors. This suggests that half of these hospitalized COVID-19 patients may have had cardiovascular comorbidities. [13, 14] Cardiac injury at admission is associated with high mortality and the need for mechanical ventilation. [16, 17] Plasma troponin and natriuretic peptide levels increase with the increasing severity of SARS-CoV-2 infection. [18] COVID-19 patients with severe symptoms experience a greater likelihood of coronary heart disease (CHD), hypertension, and diabetes compared with those with mild symptoms. [15] Patients from intensive care units (ICUs) typically have a high prevalence of CVD events and associated risk factors. For example, a retrospective analysis of 138 COVID-19 patients admitted to ICU showed that 44.4% had arrhythmias and were more likely to have underlying comorbidities, including hypertension (58.3% vs. 21.6%), diabetes (22.2% vs. 5.9%), and CVD (25.0% vs. 10.8%) compared with non-ICU patients. [14] The in-hospital mortality rate for COVID-19 patients was significantly greater among patients with myocardial injury than among those without myocardial injury (60.9% vs. 25.8%). [19] The numbers of both clinical and basic studies on COVID-19 have grown rapidly since the beginning of the outbreak. We searched approximately 2000 out of about 8000 of the most recent publications in the field from PUBMED and selected approximately 300 of the most recent articles that focused on the link between CVD and SARS-CoV-2. These diseases seldom stand alone but affect each other. One is often a risk factor for the other. This review will focus mostly on clinical observations placed within a pathophysiological context to assist our understanding of one of the deadliest diseases of the century to date.

Due to the demand for hospital resources for COVID-19 patients, social distancing requirements, and fear of infection during the pandemic, in-person clinical visits were widely switched to virtual (audio or video) consultations, telemedicine, or digital health platforms [ Figure 1 ]. The International Atomic Energy Agency conducted a worldwide survey among 909 inpatient and outpatient centers in 108 countries and reported that cardiovascular procedures decreased by 42% from March 2019 to March 2020, and by 64% from March 2019 to April 2020. The numbers of other procedures also decreased, including transthoracic echocardiography (59%), transesophageal echocardiography (76%), stress tests (78%), and coronary angiography (invasive or computerized tomography (CT); 55%) (P < 0.001 for each procedure). [20] Between January and May 2019 and January and May 2020, there were no differences in acute thoracic and abdominal aortic procedures (incidence rates ratio (IRR) = 0.96, P = 0.39) among 40 departments in Asia, Europe, and the USA, whereas a 35% decline was recorded in the numbers of elective procedures performed (IRR = 0.81, P = 0.001). [21] In Europe, among 15 centers from 12 countries, only 20,226 consecutive acute admissions to Emergency and Cardiology Departments in 2020 were due to ACS, acute HF, arrhythmia, and pulmonary embolism compared with 30,158 in 2019. Nevertheless, the risk of death was higher in 2020 (odds ratio (OR) = 4.1). [22] A retrospective multicenter registry study conducted in Novara, Italy, included 6609 patients who underwent primary percutaneous coronary intervention (PCI) in 77 European centers in 18 countries. The authors reported a significant reduction in PCIs in 2020 compared with 2019 (IRR = 0.81, P < 0.0001). [23] Data from an Italian National survey of 14 hospitals showed a 39% reduction in hospital beds for surgical patients. Additionally, compared with 2019, surgical activity decreased by 52% in 2020. That fewer procedures were performed did not obviate the risk for nosocomial SARS-CoV-2 infection. Indeed, the report stated that 29 nurses, 12 doctors, and 3 postoperative patients became infected with COVID-19. [24] In 9 locations in western Germany between January 1 and April 30, 2020, there was an overall decline of 20% in cardiovascularrelated admissions, including 53% for dizziness/syncope, 38% for HF, 28% for chronic obstructive pulmonary disease (COPD), and between January 17 and July 22, 2020, of data from the American Heart Association (AHA) COVID-19 Cardiovascular Registry showed that Black and Hispanic patients accounted for 55.5% of the COVID-19-positive hospitalized population and were much younger than their Caucasian counterparts. [48] In a community-based UK Biobank cohort of 473,555 individuals, 459 deaths were attributed to COVID-19 deaths and 2626 to other causes. Univariate regression models showed that age (OR = 2.76, P = 2.6 Â 10 À17 ), male sex (OR = 1.47, P = 1.3 Â 10 À6 ), and Black versus White ethnicity (OR = 1.21, P = 3.0 Â 10 À7 ) were independently and jointly explanatory (area under the ROC curve (AUC): 0.79) and increased the risk for COVID-19related mortality. [49] In contrast, Asian COVID-19 patients have a higher risk of developing cardiorespiratory diseases. The mortality rate was reported to be higher for Asian (adjusted odds ratio (adjOR) = 1.31), Black (adjOR = 0.93), and Hispanic patients (adjOR = 0.90) than among the White population. [48] Despite these observations, it is clear that the effect of race on SARS-CoV-2 infection can vary depending on population structure, education level, and socioeconomic factors.

COVID-19 infections result in increased hospitalization rates and greater severity of illness in patients with metabolic diseases, such as obesity and diabetes. The International Severe Acute Respiratory and Emergency Infection Consortium described rates of 17.4% and 13.4% for diabetes and obesity, respectively, among 95,966 cases. [50] However, the prevalence of diabetes and obesity was reported to be much higher (33.8% and 41.7%, respectively) in a cohort of 5700 COVID-19 patients from 12 New York City hospitals between March 1 and April 4, 2020, [51] likely because the definition of obesity differs between Asians and Americans. [52] Analysis of 7606 patients from the AHA COVID-19 registry through July 22, 2020, showed that obesity classes I to III are risk factors for in-hospital death or mechanical ventilation (OR = 1.28, 1.57, and 1.80, respectively), while class III obesity is associated with the risk of in-hospital death (HR = 1.26). Being overweight and obesity classes I to III are also risk factors for mechanical ventilation (OR = 1.28, 1.54, 1.88, and 2.08, respectively). [53] Similar observations were made in France and China. In a retrospective cohort study from the University Hospital in Lille, France, the authors reported an OR of 7.36 for a requirement for invasive mechanical ventilation among obese COVID-19 patients (body mass index (BMI) of ≥35 kg/m 2 ) versus those with a BMI of <25 kg/m 2 after adjusting for age, sex, and comorbidities such as diabetes, hypertension, and dyslipidemia. [54] A BMI greater than 28 kg/m 2 is defined as obese in China. The OR for developing severe pneumonia among 383 hospitalized COVID-19 patients was 1.84 for overweight patients (BMI 24.0-27.9 kg/m 2 ) and 3.40 for obese patients compared with those of normal weight (BMI 18.5-23.9 kg/ m 2 ). [55] Among age-and sex-matched COVID-19 patients, obesity was associated with a 3-fold increased risk of severe COVID-19 (OR = 3.00) after adjusting for age, sex, smoking status, hypertension, diabetes mellitus, and dyslipidemia. [56] A systematic review and meta-analysis of 41 studies encompassing a total of 219,543 subjects, including 115,635 SARS-CoV-2positive patients, showed that obesity was a risk factor for COVID-19 (OR = 1.50). Obese COVID-19 patients showed a higher incidence of hospitalization (OR = 1.54), ICU admission (OR = 1.48), invasive mechanical ventilation (OR = 1.47), and in-hospital death (OR = 1.14). [57] Of the total Scottish population of 5,463,300, diabetic patients accounted for 5.8% (319,349), 1082 (0.3%) of whom developed fatal or ICU-treated COVID-19; of these, 972 (89.8%) were more than 60 years old. In contrast, among the 5,143,951 non-diabetic individuals, only 4081 (0.1%) developed fatal or ICU-treated COVID-19. After adjusting for age and sex, diabetes remained a strong risk factor for fatal and ICU-treated COVID-19 (adjOR = 1.395, P < 0.0001). [58] Besides these CVD risk factors, substantial evidence exists worldwide to support the existence of a link between SARS-CoV-2 infection and CVD-associated comorbidities.

Hypertension is an important component of metabolic syndrome and a significant risk factor for CVD and SARS-CoV-2 infection. In Asia, a study from Beijing, China, showed that 81 (16%) out of 498 consecutive hospitalized COVID-19 patients had preexisting hypertension. There were more cases of severe COVID-19 among patients with hypertension than among those without hypertension (21% vs. 10%, P = 0.007). Hypertension is associated with an increased risk of illness even after adjusting for age, sex, hospital geographical location, and blood pressure at admission. Hypertensive patients were often older and had higher blood neutrophil counts and CRP, lactate dehydrogenase, and NT-proBNP levels compared with non-hypertensive patients. Like age (OR = 1.062, P < 0.001), hypertension was also a strong risk factor for COVID-19 (OR = 2.310, P = 0.008) in this Chinese population. [59] Other studies from China yielded the same conclusion. In a group of 113 confirmed COVID-19 cases from Shanghai, China, pre-existing hypertension and a high sequential organ failure assessment (SOFA) score were found to be independent risk factors for the development of cardiac injury among COVID-19 patients. [60] Furthermore, among 414 COVID-19 patients from Wuhan, China, patients with hypertension (149) had higher plasma levels of hs-cTnI (P < 0.0001) and NT-proBNP (P < 0.0001) on admission. Moreover, hypertension was found to be a risk factor for in-hospital death (HR = 2.57) after adjusting for age and sex. [61] In a study from Tehran, Iran, the authors reported that 176 (29.4%) out of 598 COVID-19 patients had underlying hypertension. Severe/critical COVID-19 was more frequent among patients with hypertension than in those without hypertension (23.8% vs. 9.7%, P = 0.012). [62] A report by the Chinese Center for Disease Control and Prevention stated that 4.2% of 44,672 patients with COVID-19 had CVD and 12.8% had hypertension. [63] Additionally, although the total fatality rate was 2.3%, that for patients with hypertension, diabetes, and CVD was 6.0%, 7.3%, and 10.5%, respectively. [64] The National Health Commission of China also reported that the mortality rates for COVID-19 patients with a history of CHD and hypertension were 17% and 35%, respectively. [5] Apart from respiratory failure, CVD-related comorbidities were the most frequently observed complications in a multicenter cohort study of 191 COVID-19 patients from Wuhan, China. [13] Cardiac injury (59% vs. 1%), HF (52% vs. 12%), and elevated concentrations of creatinine kinase-myocardial isoform (CK-MB) and hs-cTnI were more common in non-survivors than in survivors. [13] A retrospective study of 150 COVID-19 patients from Wuhan found that CVD was common in patients who died (13 out of 68) but not in those who recovered (0 out of 82). [65] A different study from Wuhan comprising 77 COVID-19 patients who were admitted to the ICU showed that those presenting with myocardial injury were generally older ((68.4 ± 10.1) years vs. (62.1 ± 13.5) years, P = 0.02), had a higher prevalence of underlying CVD (34.1% vs. 11.1%, P = 0.02), and experienced more in-ICU CV complications (41.5% vs. 13.9%, P = 0.008) than non-ICU patients. Myocardial injury at admission increased the risk of 28-day mortality (HR = 2.2, P = 0.004). [66] A study of 138 hospitalized patients with COVID-19 in Wuhan showed that the levels of markers of cardiac damage (CK-MB and hs-cTnI) were greatly increased in ICU patients compared with those not requiring intensive care, while ICU patients were more likely to manifest arrhythmia than non-ICU patients (44.4% vs. 6.9%). [6] Hospitalized COVID-19 patients (n = 416) from Wuhan also demonstrated high levels (19.7%) of cardiac injury, greater age, more comorbidities, more laboratory abnormalities, and more extensive chest X-ray findings. [17] Patients with cardiac injury were more likely to require mechanical ventilation (P < 0.001) and experienced higher mortality rates than those without cardiac injury (51.2% vs. 4.5%, P < 0.001). [17] A meta-analysis of 6 studies that included 1527 COVID-19 patients from China showed that 8% suffered from acute cardiac injury. The incidence of acute cardiac injury was 13-fold higher in ICU/ severe COVID-19 patients than non-ICU/severe patients. [6] In Korea, studies of COVID-19 patients from 10 hospitals from February 15 to April 24, 2020, showed that 42% (n = 954) had pre-existing CVD or risk factors for CVD, including hypertension (28.8%) and diabetes (17.0%). Patients with pre-existing CVD or those with risk factors for CVD experienced higher ICU admittance rates (5.3% vs. 1.6%, P < 0.001) or a greater requirement for mechanical ventilation (4.3% vs. 1.7%, P < 0.001) than those without CVD or the risk factors. CVD and associated risk factors (adjOR = 1.79, P = 0.027), diabetes (OR = 2.43, P < 0.001), and congestive HF (OR = 2.43, P = 0.049) independently predicted in-hospital death. [67] The Premier Healthcare Database from North Carolina, US, contained data for 132,312 patients hospitalized from April 1 to September 30, 2020, 8383 (6.4%) of whom were COVID-19positive. The death rate was markedly higher in these patients (24.2%) than in those hospitalized for HF (2.5%). As expected, being male (OR = 1.26), age (OR = 1.35), and obesity (OR = 1.25) were associated with in-hospital mortality among patients with HF and COVID-19. [68] Out of a large cohort of 18,472 individuals in the US registry from Ohio and Florida, between March 8 and April 12, 2020, a total of 1735 were found to be COVID-19-positive, 421 were admitted to hospital (24.3%), 161 (9.3%) were admitted to an ICU, and 111 (6.4%) experienced mechanical ventilation. Among the COVID patients, 682 (39.3%) had hypertension; 332 (19.1%) were diabetic; 161 (9.3%) had CAD; and 146 (8.4%) had HF. [69] Among another US cohort of 5894 COVID-19 patients from New York from March 1 to April 15, 2020, 2573 (40%) had a history of hypertension. [70] Of the 1533 acute myocardial infarction (AMI) patients who presented to the MedStar Health system (11 hospitals in Washington DC and Maryland), 86 had COVID-19, were older, tended to be non-White, and had more comorbidities compared with AMI patients who tested negative for COVID-19. In-hospital mortality (P < 0.001) and the levels of inflammatory markers (white blood cells, lactate dehydrogenase, ferritin, and CRP) and NT-proBNP (P = 0.044) were also higher in AMI patients diagnosed with COVID-19 than in those testing negative for the disease. [71] A chest CT-based study of 180 adult COVID-19 patients from New York found that coronary artery calcification was associated with intubation (adjOR = 3.6) and mortality (adjOR = 3.2). [72] A prospective cohort study of 586 COVID-19 patients from Yale University showed a 36.7% incidence of CVD. The highest incidence was reported for hypertension (60.2%), followed by diabetes (39.8%), and hyperlipidemia (38.6%). In this cohort, age (OR = 1.28), previous ventricular arrhythmia (OR = 18.97), use of P2Y 12inhibitors (OR = 7.91), high CRP (OR = 1.81), high hs-cTnT (OR = 1.84), and low albumin (OR = 0.64) were associated with all-cause of mortality. [73] A study of 52 COVID-19 patients from Milwaukee, Wisconsin, who were admitted to ICU reported that 29% had prior cardiac disease. These patients were more likely to have new or worsening left ventricle (LV) dysfunction, while echocardiographic analysis showed that 55.7% had cardiac complications. Prior cardiac disease, right ventricular enlargement, and pulmonary hypertension were all associated with morbidity and mortality in these COVID-19 patients. [74] An observational and retrospective study from Italy, Europe, reported that hypertension was the most prevalent comorbidity among 351 COVID-19 outpatients (35%). [75] Pre-existing hypertension increased the risk of developing severe disease and death. [76] A study of 692 consecutive patients with COVID-19 from 13 Italian cardiology centers between March 1 and April 9, 2020, showed that 13% (n = 90) had a history of HF. The inhospital death rate was higher in patients with HF (41.1%) than in those without HF history (22.9%). Multivariate COX regression analysis showed that HF history was a risk factor for COVID-19-associated death (adjusted hazard ratio (adjHR) = 2.25, P = 0.006). [77] Among 6272 patients from the Lombardy region of Italy diagnosed with COVID-19 between February 21 and March 11, 2020, 23.9% of the women and 33.8% of the men had underlying CVD. [78] A study from the same city showed that among 1043 COVID-19 patients admitted to ICU, 49% had hypertension, 21% had CVD, and 21% had diabetes. Of the patients with hypertension, 38% died in the ICU. [79] In France, a study from Paris reported the detection of coronary artery calcification in 50.7% of 209 consecutive hospitalized COVID-19 patients. These patients had more primary outcomes (50.0% vs. 17.5%, P < 0.0001) and episodes of non-invasive ventilation (49.1% vs. 15.5%, P < 0.0001) than those without calcification. [80] In Germany, among 40 COVID-19 patients from 2 medical centers, 19 had hypertension, 11 had diabetes, and 10 had previously diagnosed cardiac disease. Plasma NT-proBNP levels were elevated in 27 patients, and hs-cTnT levels in 25; of these, 18 had no prior cardiac disease. Plasma CK-MB levels were increased in 17 patients, 15 of whom had no history of cardiac disease. Free light chain immunoglobulin (FLC Ig) lambda levels were elevated in 32 patients, FLC Ig kappa levels in 29, and Ddimer levels in 33. Blood hs-cTnT levels were higher in COVID-19 patients admitted to ICU than in non-ICU COVID-19 patients. [81] In Spain, a study of 3080 COVID-19 patients from Madrid reported that patients with a previous history of HF (4.9%) were more prone to developing acute HF (P < 0.001), had higher levels of NT-proBNP, and experienced higher mortality rates (P < 0.001) than those without previous HF. COVID-19 patients with acute HF also had higher mortality rates than those without acute HF (P < 0.001). [82] Meta-analyses have given a broad overview of the associations between SARS-CoV-2 infection and CVD-related comorbidities. Patients with a history of CVD and hypertension were more likely to have elevated troponin levels. [83] A meta-analysis involving 11 studies reported a combined risk ratio (RR) of 2.49 for severe disease in the presence of hypertension. [84] An analysis of 56 studies and 198 articles that included 159,698 patients infected with COVID-19 showed that acute cardiac injury (OR = 13.29), hypertension (OR = 2.60), HF (OR = 6.72), arrhythmia (OR = 2.75), CAD (OR = 3.78), and CVD (OR = 2.61) were associated with mortality. Arrhythmia (OR = 7.03), acute cardiac injury (OR = 15.58), CHD (OR = 2.61), CVD (OR = 3.11), and hypertension (OR = 1.95) were associated with ICU admission. [85] The results of these meta-analyses indicate that there are strong links between COVID-19 and CVD across patients from Asia, America, and Europe, although the degree of association may vary depending on the study origin and sample size.

Hypertension involves endothelial dysfunction that leads to an imbalance between vasodilation and vasoconstriction, elevated levels of reactive oxygen species (ROS) and pro-inflammatory mediators, and reduced nitric oxide (NO) bioavailability. [86, 87] SARS-CoV-2 interactions with endothelial cells (ECs) in hypertensive patients drive viral-mediated injury and EC dysfunction; induce chemokine release by ECs, with consequent inflammatory cell adhesion and migration through the endothelial barrier; and lead to a procoagulant state and tissue damage, such as myocardial injury. [88] SARS-CoV-2 infection is mediated by ACE2 located on the host cell surface. [89] ACE2 is a homolog of the metalloproteinase ACE, a key enzyme in the reninangiotensin system (RAS) that produces angiotensin II (Ang II). [90] [91] [92] Ang II promotes EC dysfunction, while SARS-CoV-2 infection-mediated downregulation of ACE2 expression results in the dysregulation of the renin-angiotensin-aldosterone system (RAAS). [93] In the RAAS, ACE converts Ang I into Ang II, which can bind to both Ang II receptor 1 (AGTR1) and AGTR2. In contrast, ACE2 hydrolyzes Ang I into Ang-(1-9) and also generates Ang-(1-7) from Ang II. Ang-(1-7) binds to both AGTR2 and Mas receptor (MasR), an endogenous orphan receptor for Ang-(1-7) [ Figure 2 ]. [94] ACE/Ang II/AGTR1 mediate vascular dysfunction, while ACE2/Ang-(1-7)/AGTR2/ MasR form the vasoprotective axis that leads to vasodilatory, anti-fibrotic, anti-proliferative, and anti-inflammatory effects. [95] [96] [97] [98] Both the overactivity of the vasodeleterious axis and the hypoactivity of the vasoprotective axis are major contributors to hypertension. In addition to producing Ang-(1-7) from Ang II, ACE2 also produces Ang-(1-9) from Ang I, which is then converted into Ang-(1-7) by ACE [ Figure 2 ], indicative of the complexities of ACE and ACE2 functions in the RAAS. A cardiac ACE-mediated increase in Ang II levels drives LV hypertrophy. [99, 100] Patients with hypertension are particularly susceptible to an imbalance between the ACE/Ang II/AGTR1 and the ACE2/Ang-(1-7)/AGTR2/MasR axes, which may be further intensified by SARS-CoV-2-mediated downregulation of ACE2 in the myocardium and endothelium. [93, 101, 102] SARS-CoV-2 infection shifts the balance in the RAAS toward the deleterious axis, resulting in elevated oxidative stress and inflammation in both the endothelium and myocardium. Ang II acts on AGTR1 and promotes the expression of pro-inflammatory cytokines and chemokines via nuclear factor kappa B (NF-kB), [103] favoring vascular permeability and inflammatory cell accumulation, and resulting in tissue injury and hypoxia [ Figure 2 ]. [104, 105] By binding to AGTR1, Ang II promotes vasoconstriction, profibrotic and pro-thrombosis responses, and cell proliferation. AGTR1 also stimulates nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and ROS production, which impairs endothelial NO synthase function and reduces NO production, thereby facilitating thrombosis and vascular inflammation [ Figure 2 ], which are features of hypertension-associated chronic endothelial dysfunction. [106] [107] [108] Thus, AGTR1 activation contributes to the development and progression of hypertension. [99, 109, 110] ACE2 acts as a counter-regulatory enzyme by converting Ang II into Ang-(1-7), a heptapeptide that binds to both AGTR2 and MasR to reduce blood pressure, [95, 97] promote vasodilation, increase kidney Na and water excretion, and elicit antiinflammatory, anti-proliferative, antioxidant, and anti-apoptotic effects that benefit CVD [ Figure 2 ]. [111] [112] [113] [114] These activities oppose the ACE/Ang II/AGTR1 axis. ACE2/Ang-(1-7)/AGTR2/ MasR signaling stimulates the activity of endothelial NO synthase, increases NO production, decreases Ang II-stimulated NADPH oxidase activity, and modulates the generation of reactive ROS [ Figure 2 ]. [115] These activities of Ang-(1-7) can ameliorate hypertrophy and fibrosis in mice. [116] ACE2 overexpression prevents or even reverses HF-related features, [117] [118] [119] [120] whereas ACE2 deficiency exacerbates aspects of HF. [121] SARS-CoV-2 infection reduces ACE2 expression and Ang-(1-7) production, leading to reduced Ang-(1-7)-mediated cardioprotective activity and increased Ang II levels. [96] Whereas ACE2 overexpression ameliorates cardiac remodeling, [95] the loss of ACE2 in endothelial and cardiac cells may contribute to acute and, perhaps, chronic exacerbation of CVD in SARS-CoV-2infected hypertensive patients. Prolonged systemic hypertension results in associated target organ damage, such as LV hypertrophy, which is also an independent risk factor for cardiovascular complications.

The ACE/Ang II/AGTR1 and ACE2/Ang-(1-7)/AT2R/MasR pathways are co-expressed in most tissues and act in both autocrine and paracrine manners. ACE2 is relatively abundantly expressed in the heart, mainly in pericytes and cardiomyocytes, and at much lower levels in ECs and fibroblasts, [93, [122] [123] [124] [125] [126] [127] [128] [129] [130] [131] [132] [133] [134] [135] [136] although some studies have reported that ACE2 is not expressed in human cardiac ECs. [137] SARS-CoV-2 infection downregulates ACE2, leading to disrupted Ang II metabolism [138] and vascular permeability, [139] increased Ang II availability and Ang-(1-7) deficiency, increased inflammation, endothelial activation, leukocyte recruitment, and even platelet activation [ Figure 2 ]. [140] Through a mechanism that likely involves the NF-kB pathway, Ang II enhances inflammatory cytokine production, cellular apoptosis, and fibrosis in response to hypoxia. [97] The reduced presence of ACE2 on the cell surface might be a result of endocytosis and intracellular proteolysis of membrane-bound ACE2. [95] In cardiomyocytes, ACE2-mediated SARS-CoV-2 infection causes myocardial injury through the downregulation of cardioprotective gene expression. [128, [141] [142] [143] Reduced ACE2 expression in cardiac tissue in COVID-19 patients is associated with an increase in Ang II availability as a result of disrupted Ang II metabolism. Plasma Ang II levels correlate with the viral load. [144] Combined, these observations indicate that SARS-CoV-2 infection worsens outcomes following myocardial injury.

Ace2 gene transfer attenuates atherosclerosis in mice by reducing angiogenesis and regulating monocyte-EC interaction via decreasing the expression of adhesion molecules in ECs through Ang-(1-7) production. [134, 135] Blood pressure is also increased in ACE2-deficient mice. [145] Lentiviral-mediated overexpression of ACE2 improved blood pressure and hypertensionassociated pathologies. [146] Vascular ACE2 overexpression increased Ang-(1-7) production, restored endothelial function, and reduced blood pressure. [147] Although the underlying mechanisms are unknown, compared with that in their wildtype siblings, blood pressure remains unchanged in 3-month-old ACE2-deficient mice, or is even lower after 6 months. [148] MasR null mice also have normal blood pressure. [149] In wild-type mice, treatment with recombinant human ACE2 did not affect baseline blood pressure or plasma levels of Ang II or Ang-(1-7). [150] These conflicting observations remain to be confirmed and explained.

Cardiac injury and myocarditis SARS-CoV-2 infection via alveolar epithelial cells causes neutrophil accumulation and enhances sub-endothelial space vascular permeability, which results in alveolar exudate formation, [151, 152] pulmonary edema, alveolar gas exchange disorders (such as acute respiratory distress syndrome (ARDS), oxygen depletion, and hypoxia), [153, 154] consequently leading to myocardial infarction, sudden cardiac arrest, HF, and abnormal coagulation. Severe pneumonia due to SARS-CoV-2 infection impairs gas exchange and causes hypoxemia, explaining why critically ill COVID-19 patients usually require oxygen therapy and mechanical ventilation for respiratory support. SARS-CoV-2 infection-induced hypoxic injury triggers pulmonary vasoconstriction and pulmonary hypertension, leading to cardiac insufficiency and HF. [155, 156] Systemic and local increases in cytokine concentrations can cause myocardial injury, [157] characterized by increased troponin levels that precede myocardial ischemia or non-ischemic processes such as myocarditis. [158] In France, post-mortem histological analysis of hearts from patients with SARS-CoV-2-induced myocarditis showed inflammatory cell accumulation involving mainly macrophages and CD8 + T cells in the ventricles and septum. All nasopharyngeal swabs and distal bronchoalveolar lavages tested negative for SARS-CoV-2 RNA before death. Additionally, myocardial cells in these patients were positive for anti-SARS-CoV2 antibodies. [159] Biopsies of COVID-19 patients from China showed myocardium infiltration of the same mononuclear cells but not CD4 + T cells. [157] Persistent myocarditis led to dilated cardiomyopathy and increased the risk of mortality [ Figure 3 ]. [160] Hs-cTnI, hs-cTnT (cardiac injury), and NT-proBNP (myocardial stress) are biomarkers with prognostic value in COVID-19 patients. Their plasma levels are known indicators of cardiac injury. Initial examination showing increased levels of hs-cTnI and NT-proBNP predicts mortality in COVID-19 patients. [161] Plasma troponin levels are generally higher in patients with severe COVID-19 than in those with mild disease. [18] Across studies, between 7% and 36% of hospitalized COVID-19 patients display elevated cardiac hs-cTnI or hs-cTnT concentrations, and high levels of troponin are associated with the risk for ICU admission and mortality. [14, 17, 83] In several Chinese cohorts, markers of myocardial injury were found in 7% to 17% of COVID-19 patients [13, 14, 151] and were present more frequently in those admitted to ICU (22.2% vs. 2.0%, P < 0.001) or who died (59% vs. 1%, P < 0.0001). [13, 162] A cohort study from Wuhan, China, showed that myocardial injury was common among COVID-19 patients and was associated with in-hospital mortality. [17] Out of 416 hospitalized COVID-19 patients, 82 (19.7%) had cardiac injury and exhibited higher plasma levels of NT-proBNP, CK-MB, and hs-cTnI compared with patients without myocardial injury. The mortality rate was also 10-fold higher in patients with cardiac injury than in those with normal cardiac function (51.2% vs. 4.5%, P < 0.001). A COX regression model also demonstrated that mortality rates were higher both during the time from symptom initiation and admission to the endpoint. [17] A study of 173 COVID-19 patients (119 over and 54 below 60 years of age) from Tongji Hospital, Wuhan, China, showed that age, but not sex, was positively correlated with cardiac injury (hs-cTnI). Multivariate logistic regression analysis showed that increased blood levels of procalcitonin, IL-2 receptor, IL-6, IL-10, TNF-a, CRP, and D-dimer; higher white blood cell and neutrophil counts; and lower blood lymphocyte and natural killer (NK) cell counts were all associated with cardiac injury. [163] In another 150 COVID-19 patients from the same hospital, including 16 mild and 24 severe cases, blood NT-proBNP, hs-cTnI, hs-CRP, and creatinine levels were all higher in patients with severe COVID-19 than in those with mild disease. [164] Meanwhile, a study of 100 patients with confirmed severe COVID-19 from February 8 to April 10, 2020, from Beijing Hospital found that plasma hs-cTnI levels were higher in individuals aged > 60 years than in those aged 60 years (median interquartile range (IQR): 5.2 vs. 1.9, P = 0.018). Hs-cTnI levels were also higher in men than in women (IQR: 4.2 vs. 2.9, P = 0.018), as were plasma NT-proBNP levels (32.1% vs. 9.1%, P = Cardiology Discovery (2021) 1 4 www.cardio-discovery.org 0.006), although no differences were found between elder and young patients. [91] An international multicenter cohort study involving 305 patients (205 of whom were male) hospitalized with laboratory-confirmed COVID-19 from 7 hospitals in New York City and Milan identified myocardial injury in 190 (62.3%) of them. Inhospital mortality was higher in patients with myocardial injury with (31.7%) or without (18.6%) transthoracic echocardiographic abnormalities than in those without myocardial injury (5.2%). [165] A study from New York University comprising 2163 consecutive COVID-19-positive adult patients with hs-cTnI ≥ 1 m g/mL showed that patients with myocardial injury were older, more likely to be male, and were associated with higher inhospital mortality and a greater frequency of critical illness compared with those without myocardial injury. [166] A prospective study of 32 hospitalized COVID-19 patients from Munich, Germany reported that 65.7% of patients had left and/or right ventricular dysfunction. Concomitant biventricular dysfunction was common in patients with increased hs-cTnT levels. [167] In a meta-analysis of 13 studies encompassing a total of 3289 COVID-19 patients, the presence of cardiac injury was found to be associated with mortality (RR = 7.95, P < 0.001), the need for intensive care (RR = 7.94, P = 0.01), and more severe COVID-19 (RR = 13.81, P < 0.001). [168] The mechanisms by which SARS-CoV-2 infection increases the risk of myocardial injury and myocarditis can be multifactorial, and can involve hypoxemia and oxygen supply and demand mismatch, direct infection of the myocardial tissue by SARS-CoV-2, cardiac damage due to an inflammatory cytokine storm, oxidative stress, microvascular dysfunction, plaque instability, and coagulation abnormalities [ Figure 3 ]. [169] SARS-CoV-2 enters cardiomyocytes via ACE2 [128] and causes direct damage to the host cells. Endomyocardial biopsy showed cardiomegaly and scattered cardiomyocyte necrosis and mononuclear cell infiltration in the myocardium. [170] Viral particles have been detected in cardiac tissues from COVID-19 patients. [126, 127, 171, 172] SARS-CoV-2 impairs stress granule formation, thereby facilitating viral replication and cellular damage. [173] Following entry, the virus activates inflammatory responses, leading to mononuclear cell and T-cell accumulation. Primed CD8 + T cells cause cardiomyocyte inflammation and cytotoxic cell-mediated toxicity. Pro-inflammatory cytokines are released into the circulation, thereby augmenting lymphocyte activation and cardiomyocyte damage. [158, 174] Cellular hypoxia may also worsen cardiomyocyte injury. COVID-19 patients show various degrees of hypoxia, which reduces energy supply for cell metabolism and increases anaerobic metabolism, intracellular acidosis, and oxygen free radical generation. [151] Hypoxia also induces calcium influx, which promotes cardiomyocyte apoptosis. [6] ACS ACS includes STEMI, non-STEMI, and unstable angina. Increased thrombotic tendency as reflected by elevated plasma D-dimer levels can promote AMI in COVID-19 patients. [175] An increase in D-dimer levels, which is indicative of fibrin formation, has been used as a biomarker for hypertension and AMI, [175, 176] and is also a prognostic marker for mortality in COVID-19. [176] One study of 199 COVID-19 patients reported an association between D-dimer values >1 mg/mL and in-hospital mortality (adjHR = 18.4). [13] In a retrospective cohort of 191 patients with COVID-19 from Wuhan, China, 15 (8%) were found to have CHD. [13] The National Health Commission of China reported that 17% of COVID-19 patients had CHD. [5] Local inflammation and hemodynamic changes may increase the risk of atherosclerotic plaque rupture, resulting in AMI [ Figure 3 ].

During the pandemic surge in 2020, STEMI catheterization procedures fell by 40% in Spain and 38% in the US. [177, 178] These drops were associated with multiple factors, including reduced numbers of hospital visits due to patient reluctance, increased medical reperfusion, reduced daily stressors due to social distancing and quarantine, less pollution from traffic and factories, more rest and fewer activities, and, perhaps, less smoking due to warnings from social media. [177] However, case studies showed that delayed STEMI presentation may have increased the risk of mechanical complications associated with AMI. [179] The European Society of Cardiology (ESC) recommends a maximum delay from STEMI presentation to reperfusion of less than 120 minutes for COVID-19 patients. Although non-STEMI ACS is an urgent condition, emergent PCI is usually not necessary. The ESC divided non-STEMI into 4 groups based on the risk level. High-risk non-STEMI should follow the STEMI treatment strategies, whereas intermediate and low-risk non-STEMI may use non-invasive strategies. [178, 180] Cardiomyopathy and HF HF is one of the most common complications of COVID-19 and is more likely to occur in patients with pre-existing cardiac disease. [181] Patients with HF exhibit high ACE2 expression and an increased risk of heart attack that progresses after infection. [122] Studies have shown that SARS-CoV and MERS-CoV infections cause and aggravate HF. [7, 182, 183] NT-proBNP is a quantitative cardiac biomarker for hemodynamic stress and HF. High NT-proBNP (>88.64 pg/mL) levels are associated with high mortality rates in COVID-19 patients. [184] Numerous studies have shown HF occurs frequently among COVID-19 patients. Among those who died from SARS-CoV-2 infection, nearly 50% had HF. [16, 17, 175] For example, in a retrospective case series of 799 moderate to severely ill COVID-19 patients from Tongji Hospital in Wuhan, China, 41 (49%) out of 83 deceased patients had HF. [181] Another retrospective multicenter cohort study from Wuhan showed that HF occurred in 44 (23%) out of 191 COVID-19 patients and 28 (52%) out of 54 nonsurvivors. [13] A descriptive study of 99 COVID-19 patients from Wuhan again revealed similar levels of HF in patients who died. HF contributed to 40% of deaths in this study. Among the 99 cases, 2 had no history of CVD but nevertheless died of HF and sudden cardiac arrest. [185] Studies from the US and other countries have yielded similar conclusions. A retrospective study of 6439 patients with COVID-19 from Mount Sinai Hospital in New York demonstrated that patients with previous HF experienced longer hospital stays (P < 0.001), had a higher risk of requiring mechanical ventilation (adjOR = 3.64, P < 0.001), and had higher mortality rates (adjOR = 1.88, P = 0.002) compared with those without previous HF. [186] An international study comprising 1272 COVID-19 patients from 69 countries reported that 39% of hospitalized patients had an LV abnormality (dilation, systolic dysfunction, diastolic dysfunction), 33% had a right ventricle abnormality, and 28% experienced biventricular failure. [60] Many HF patients developed COVID-19 and COVID-19 patients are at increased risk of HF. These observations are suggestive of an interconnection between SARS-CoV-2 and HF pathogenesis. Besides a direct effect of SARS-CoV-2 on cardiomyocytes that leads to cardiomyocyte apoptosis or necrosis, [126] [127] [128] [141] [142] [143] 171] a cytokine storm following SARS-CoV-2 infection can induce and aggravate HF, similar to that seen with SARS-CoV and MERS-CoV. [187] Although the direct underlying mechanisms remain unknown, IL-6 released during a cytokine storm can induce diastolic dysfunction by exerting positive inotropic effects on cardiomyocytes via the JAK/STAT3 pathway [188] and increase cardiomyocyte stiffness by reducing the phosphorylation of titin. [189] IL-1b and TNF-a cause contractile abnormalities in cardiomyocytes by altering intracellular Ca 2+ homeostasis [190, 191] and induce cardiomyocytes apoptosis. [192] IL-1b and TNF-a upregulate AGTR1 expression in cardiac fibroblasts and promote Ang II-induced collagen deposition and fibrosis. [193] TNF-a promotes fibrosis by enhancing the expression of the HF biomarker lysyl oxidaselike 2 via the upregulation of TGF-b. [194] These mechanisms may all contribute to the pathogenic effects of SARS-CoV-2 in human HF [ Figure 3 ].

Arrhythmia can arise secondary to hypoxemia, metabolic disorders, and systemic inflammation following viral infection in patients with or without pre-existing CVD [ Figure 3 ]. [175] Arrhythmia includes atrial fibrillations, ventricular tachycardia, and ventricular fibrillation, all symptoms that have been reported in COVID-19 patients. [14, 16, 195] Patients with severe COVID-19 and with established or undiagnosed CVD are more prone to developing arrhythmia in response to any illness, including SARS-COV-2-induced ischemia or myocardial injury, indirect effects from hypoxia, septic shock, multi-organ failure, and metabolic and electrolyte abnormalities. [196] Arrhythmia occurs in approximately 5% to 17% of COVID-19 patients. [16, 195, 197] Two independent studies from Zhongnan Hospital in Wuhan, China, reported that among 138 hospitalized COVID-19 patients, 23 had cardiac arrhythmia. Patients with cardiac arrhythmia were more prevalent in the ICU than in non-ICU settings (44.4% vs. 6.9%, P < 0.001). [14] A retrospective case series of 102 COVID-19 patients reported that COVID-19 patients admitted to ICU were more likely to suffer from arrhythmia (ICU 38.9% vs. non-ICU 13.1%). [198] A study from 8 centers in Heidelberg, Germany, indicated that 20.5% of 166 hospitalized COVID-19 patients had arrhythmia. Atrial fibrillation was the most common observation, while age and CVD were predictors of new onset. Arrhythmia has been associated with increased levels of cardiac biomarkers, hospitalization, admission to ICU, mechanical ventilation, and in-hospital mortality. In multiple regression analyses, the incidence of arrhythmia was associated with hospitalization duration and mechanical ventilation. Blood hs-cTnT (P < 0.001), NT-proBNP (P < 0.001), IL-6 (P = 0.014), lactate dehydrogenase (P = 0.012), hospital duration (P < 0.001), admission to ICU (P = 0.025), and duration in ICU (P = 0.025) were all significantly higher in patients with arrhythmia (n = 34) than in those without arrhythmia (n = 132). [199] In a study of 700 COVID-19 patients from Philadelphia, 53 (8%) were found to have developed arrhythmia-related events during hospitalization, including 9 cardiac arrests, 25 atrial fibrillations, and 9 clinically significant bradyarrhythmias, while 10 patients experienced non-sustained ventricular tachycardias. [195] A retrospective analysis of 4526 hospitalized COVID-19 patients from 4 continents and 12 countries found that 827 had arrhythmia; of these, 69% had hypertension, 42% had diabetes mellitus, 30% had HF, and 24% had CAD. Among those who developed arrhythmia, 81.8% showed atrial arrhythmia, 20.7% had ventricular arrhythmia, and 22.6% had bradyarrhythmia. [200] Thrombosis and platelet activation Compared with influenza patients, COVID-19 patients have 9 times as many alveolar capillary microthrombi, leading to a significantly greater number of capillary occlusions. [201] Thrombosis in the lung induces pulmonary hypertension, leading to increased levels of cardiac troponin, CK-MB, and NT-proBNP in patients with severe COVID-19. [14, 17, 102, 202] Therapeutic anticoagulant treatment can reduce mortality among COVID-19 patients, highlighting the importance of thrombosis in SARS-CoV-2 infection. [203] ECs express ACE2. [129] [130] [131] [132] [133] [134] [135] 203] IL-6 and hepcidin enhance ACE2 expression in human pulmonary artery ECs [131] and these 2 molecules are strongly correlated with SARS-CoV-2 infection severity. [204] [205] [206] Virus infection causes EC death and consequent inflammatory cell infiltration and microvascular pro-thrombotic events. In a study of 7 COVID-19 patients, the most frequently occurring severe arterial thrombotic events were limb ischemia and floating thrombus of the aorta. [207] The pro-thrombotic state found in COVID-19 patients is related to endothelial damage. SARS-CoV-2 also promotes the transformation of ECs from an anti-thrombotic to a pro-thrombotic state in the microvascular environment, leading to increased levels of von Willebrand factor (VWF) and plasminogen activator inhibitor, a major inhibitor of fibrinolysis [ Figure 4 ]. [208] Microvascular thrombosis and endothelial damage in COVID-19 patients contribute to microvascular ischemia, which increases the frequency of myocardial injury and stroke. [209, 210] COVID-19 case studies have reported the occurrence of ischemic arterial events such as intra-aortic thrombi, MI, and spontaneous thrombosis of the aortic valve. [211] COVID-19 patients showed left subclavian artery thrombosis and ulcerated plaques with floating thrombus within the aortic arch, and acute ischemia and occlusion of the superficial femoral artery. Computed tomography angiography of the abdominal aorta and lower limbs from one case study revealed the presence of multiple intra-aortic thrombi, occlusion of the distal superior mesenteric artery, splenic and renal ischemic lesions, and occlusion of the right superficial femoral artery and the left supra-articular popliteal artery. Some patients showed acute occlusion of the proximal left circumflex artery with a high thrombus load, or with normal ECG but with high blood troponin and CRP, and acute occlusion of the M2 segment of the middle cerebral artery. [211] Aberrant coagulation makes a substantial contribution to ischemic heart disease and stroke [ Figure 4 ]. COVID-19 patients exhibit abnormal coagulation parameters, such as prothrombin time, fibrin degradation products, activated partial thromboplastin time, and D-dimer values. Increased levels of fibrin degradation products and D-dimer correlated with poor prognosis. [212, 213] An observational study of 184 critically ill COVID-19 patients revealed a 31% incidence of thrombotic complications, including 4% arterial and 27% venous. [214] The levels of factor VIII and fibrinogen were elevated in COVID-19 patients, supporting a hypercoagulable state. [215, 216] Rises in D-dimer levels in COVID-19 patients indicate ongoing thrombosis. [217] Several studies from Wuhan, China, showed that elevated plasma D-dimer levels are associated with poor prognosis. Blood D-dimer values greater than 2 mg/mL may represent the cut-off value for predicting mortality (sensitivity 92.3%, specificity 83.3%). [176] The burden of underlying coagulopathy reached 50% among deceased COVID-19 patients, and D-dimer levels >1 mg/mL (normal: <0.5 mg/mL) served as an independent predictor of an 18-fold greater risk of inhospital mortality (OR = 18.4, P = 0.001). [13] Fibrin degradation products and D-dimer levels were also significantly higher in COVID-19 non-survivors than in survivors, and 71.4% of nonsurvivors had disseminated intravascular coagulation during the course of their disease. [212] Patients with high D-dimers values were more likely to require high-flow oxygen, anticoagulation therapy, antibiotics, and ICU care. These patients also had elevated levels of IL-6, greater numbers of monocytes and lymphocytes, and a greater risk of death. [218] An early report on 1099 COVID-19 patients from 552 hospitals from 30 provinces in China indicated that 46% of these patients had elevated D-dimer levels (>0.5 mg/mL), 60% of whom were severely ill. [15] Among the 150 COVID-19 patients in ICU in a French tertiary hospital, 95% had elevated D-dimer and fibrinogen concentrations. [219] Platelet activation controls thrombosis. [220] SARS-CoV-2/ platelet interactions result in platelet activation and degranulation, thereby potentiating the pro-thrombotic vascular milieu. [221] SARS-CoV-2 RNA has been detected in platelets of COVID-19 patients. These platelets were hyperactivated and aggregated under low-level thrombin stimulation. [222] SARS-CoV-2 RNA likely interacts with platelets via Toll-like receptor 7 (TLR7) and TLR9, thereby activating leukocytes and stimulating inflammatory cytokine expression [ Figure 4 ]. [223] Platelet activation and platelet-monocyte aggregate formation were detected in patients with severe, but not mild, COVID-19. [224] Platelets release chemokines (CXCL1, 5, 7 and CCL3, 5, 7), [225] providing additional mechanisms for recruiting mononuclear cells and lymphocytes as part of the cytokine storm [ Figure 4 ]. Platelets from patients with severe COVID-19 were shown to induce tissue factor expression in monocytes from healthy volunteers ex vivo. [224] A study of 36 COVID-19 patients and 31 age-and sex-matched controls from Italy showed that blood Ddimer levels were higher in the former (P < 0.001), but blood neutrophil and platelet counts did not differ. In contrast, the levels of blood platelet activation markers (P-selectin, platelet- derived microparticles, and CD66b + CD41 + platelet/neutrophil complexes) were all higher in COVID-19 patients, as were the levels of blood neutrophil activation markers (neutrophil microparticles, myeloperoxidase (MPO)/DNA complexes, citrullinated histone H3, and matrix metalloproteinase 9). In vitro, plasma from COVID-19 patients induced neutrophil MPO-DNA complex formation, which could be blocked by aspirin. [226] Many studies have also reported that blood platelet counts are reduced in COVID-19 patients. A meta-analysis that included 9 studies comprising 1779 COVID-19 patients showed that blood platelet counts were lower in patients with severe disease, and even lower in those who died. [227] A retrospective study of 1476 consecutively admitted COVID-19 patients during the pandemic in Wuhan, China, showed that platelet counts were reduced in 20.7% of them. In-hospital mortality rates of 92.1%, 61.2%, 17.5%, and 4.7% among these patients correlated with platelet counts of 50 Â 10 9 /L, >50 to 100 Â 10 9 /L, >100 to 150 Â 10 9 / L, and >150 Â 10 9 /L, respectively. [228] There was also a disparity between survivors and non-survivors, with very low platelet counts being associated with increased mortality. [228] [229] [230] VTE VTE, such as deep vein thrombosis, causes pulmonary embolism [231] and cardiac arrest. [232, 233] VTE-associated pulmonary damage and impaired gas exchange lead to an imbalance of myocardial oxygen supply/demand, reduced activity of the mitochondrial electron transport chain, acidosis, and oxidative damage [ Figure 5 ]. [234] SARS-CoV-2 may drive thrombotic processes by reducing ACE2 and Ang II clearance. In turn, increased Ang II availability promotes the release of VWF from ECs as well as platelet activation [ Figure 5 ]. [235] Patients with coexisting STEMI and COVID-19 showed increased rates of thromboembolic complications that affect multiple vessels, stents, and thrombus grade post-PCI. [236] A case study showed that COVID-19 patients with VTE had greatly elevated CRP (180 mg/L), hs-cTnI (3.24 mg/mL), and D-dimer (21 mg/mL) levels, indicating that SARS-CoV-2 infection and VTE contribute to myocardial injury and that a close association exists between COVID-19 and VTE incidence [ Figure 5 ]. [237] A study from Wuhan, China, enrolled 81 COVID-19 patients from the ICU of a local hospital, 25% of whom had VTE. These COVID-19 patients with VTE were older and exhibited abnormal coagulation parameters, such as higher D-dimer levels and longer activated partial thromboplastin time than patients without VTE. Blood D-dimer levels greater than 1.5 mg/mL (normal range: <0.5 mg/mL) predicted VTE with a sensitivity of 85.0% and specificity of 88.5%. [238, 239] A study from Leiden, Netherlands, showed that patients with severe COVID-19 had a higher incidence of thromboembolic complications. Among 184 Figure 5 : SARS-CoV-2 infection causes venous thromboembolism (VTE). SARS-CoV-2 infection reduces ACE2 expression concurrently with Ang II accumulation, which activates platelets and endothelial cells (ECs). EC-derived VWF and factor VIII contribute to VTE formation, which induces myocardial injury and pulmonary embolism as a mechanism for AMI, stroke, and HF. ACE: Angiotensin-converting enzyme; AMI: Acute myocardial infarction; Ang: Angiotensin; CRP: C-reactive protein; HF: Heart failure; hs-cTnT: High-sensitive cardiac troponin T; SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2; VWF: von Willebrand factor. Cardiology Discovery (2021) 1 4 www.cardio-discovery.org COVID-19 patients admitted to the ICU, the rate of thrombotic complications reached 31%, including 27% cases of VTE and 3.7% arterial thrombotic events. [214] After 17 days, the cumulative incidence of thrombotic events reached 49%. Pulmonary embolism was the most frequently reported thrombotic event (87%). [240] A study from Lille, France, showed that the diagnosis of pulmonary thromboembolism in 196 ICU patients with COVID-19 was high (21%), and substantially more common than the 7% for influenza patients or the 6% for all ICU patients. [241] Among a single-center cohort of 198 hospitalized patients from Amsterdam, The Netherlands, 173 patients were confirmed COVID-19-positive, 75 of whom were admitted to the ICU. These ICU patients had higher D-dimer levels, a higher percentage of VTE, deeper venous thrombosis, and increased incidence of symptomatic VTE than patients admitted to the regular ward. [242] When total confirmed cases were considered, 21% of patients had VTE, similar to those from Leiden, [214] Wuhan, [238, 239] and Lille. [241] VTE was associated with increased mortality before (HR = 2.7) and after (adjHR = 2.4) adjusting for age, sex, and ICU stay as time-varying variables. [242] A systemic review of 20 studies identified a weighted mean prevalence of 31.3% for VTE among a cohort of 1988 COVID-19 patients. [243] Other types of thrombotic events affecting the arterial system have also been reported, such as MI [15] and stroke. [244] Thrombotic events are consistently associated with a high risk of mortality. [15, 240, 245] 

Several mechanisms have been proposed to explain the coronary complications in COVID-19 patients, including coronary plaque rupture, cytokine storm, hypoxic injury, coronary spasm, microthrombi, and endothelial injury. Cardiomyocytes, ECs, and pericytes are among the best-studied cardiac and vascular host cells targeted by SARS-CoV-2 [ Figure 6 ]. SARS-CoV-2 RNA has been found in the blood of COVID-19 patients and was positively correlated with COVID-19 severity. [246] Histological analysis identified the presence of SARS-CoV-2 in myocardium from COVID-19 patients. [171, 172] RNA sequencing analysis demonstrated that ventricular cardiomyocytes expressed high levels of cathepsin L and B, which serve for SARS-CoV-2 spike (S) protein priming. [247] Cultured human-induced pluripotent stem cell-derived cardiomyocytes were shown to be susceptible to SARS-CoV-2 infection, which resulted in cytotoxicity. [126] [127] [128] 143] Direct infection of cardiomyocytes triggers cardiomyocyte inflammation, apoptosis, and necrosis, resulting in myocardial injury and myocarditis [ Figure 6 ]. Although the exact mechanisms by which SARS-CoV-2 damages cardiomyocytes remain incompletely understood, a SARS-CoV-2-induced cytokine storm is thought to be one of the mechanisms underlying COVID-19-induced cardiac injury, coronary spasm, and microthrombi, as discussed above. Another possible mechanism involves a SARS-CoV-2-induced increase in oxygen demand by cardiomyocytes during ARDS as a consequence of hypoxia, which causes oxidative stress. [248] The vascular endothelium is among the frontline targets for SARS-COV-2 infection. [249] Indeed, ECs in small or large arteries and veins express ACE2, [129] [130] [131] 134, 135, 250] to which the viral S protein binds, although at much lower levels than that seen on pericytes or cardiomyocytes. [122] [123] [124] [125] [126] [127] Viral inclusion structures were found in ECs from glomerular capillary loops and airway microvessels from patients with severe COVID-19. In these patients, the lung endothelium was severely injured, with disrupted cell membranes and a high degree of microthrombosis. [251] SARS-CoV-2 infection induces systemic inflammation. The accumulation of inflammatory cells in the endothelium leads to endothelialitis and EC apoptosis, resulting in endothelial dysfunction [ Figure 6 ]. [251, 252] Circulating cytokines increase the expression of adhesion molecules and chemokines in ECs, thereby augmenting inflammation by promoting leukocyte recruitment [ Figure 6 ]. A case study confirmed SARS-CoV-2 infection of the pulmonary endothelium concomitant with pulmonary endothelialitis and microvascular thrombosis. [201] Endothelial injury and dysfunction in COVID-19 patients may arise either from the direct infection of SARS-CoV-2 and the subsequent induction of intracellular oxidative stress [111, [253] [254] [255] or indirectly as a consequence of an acute inflammatory response. [131, [204] [205] [206] Following viral infection, endothelial activation and increased adhesion molecule expression led to enhanced neutrophil activation and ROS production. [256] Oxidative stress due to an imbalance between ROS (or free radicals) and antioxidants causes an increase in pro-thrombotic and cell adhesion molecule-related expression. [257] ROS accumulation promotes oxidative stress and NF-kB signaling, which favors vasoconstriction and vascular permeability [Figure 6] . [258, 259] SARS-CoV-2 infection of vascular ECs also results in systemic inflammatory endothelial disorder, including the leakage of plasma components from microvessels, intramicrovascular blood clotting, thrombus formation, and excessive release of inflammatory cytokines following vascular endothelial damage. [126, 127, [129] [130] [131] 250, 254, 255] Elevated cytokine levels dampen the anti-thrombotic effects of ECs by activating the coagulation system and causing thrombosis. [260, 261] In patients with severe COVID-19, the levels of endothelial and platelet activation markers remain high [262] and the resulting endothelial dysfunction promotes microthrombosis accompanied by thrombocytopenia and elevated blood D-dimer levels. [13] Pericytes reside outside of the endothelium, share a basement membrane with ECs, thereby helping to maintain basement membrane and vascular barrier integrity, and provide mechanical support to preserve EC stability and function in capillary vessels. [122] A breakdown of the pericyte-EC cross-talk results in a compromised vasculature that is prone to the inflammatory and procoagulant states. This is supported by observations from a pericyte SARS-CoV-2 infection model and COVID-19 patient histology. [136] Pericyte-deficiency (Pdgfb ret/ret mice) led to dilated capillaries and elevated production of VWF by ECs, which promoted platelet adhesion and blood coagulation by binding to and stabilizing factor VIII. The loss of pericytes promoted VWF expression in microvascular ECs, platelet aggregation, and fibrin deposition, and disrupted thrombogenic homeostasis [Figure 6] . [136] In addition to ACE2, [123, 136] cardiac pericytes also express CD147, an extracellular matrix metalloproteinase inducer that also serves as a receptor for SARS-CoV-2. [263] Cardiac pericytes are also primary SARS-CoV-2 targets. [122] SARS-CoV-2-induced pericyte injury may impair endothelial function and induce microvascular dysfunction. One study showed that recombinant SARS-CoV-2 S protein induced cardiac pericyte migration, reduced pericyte activity related to its role in supporting EC network formation, and promoted the secretion of pro-inflammatory molecules as part of the cytokine storm, as well as the production of pro-apoptotic signals targeting ECs [ Figure 6 ]. [263] Inflammation and the cytokine storm in COVID-19 patients An analysis of the pathology of the first patient who died of COVID-19 showed myocardial inflammation and damage, myocardial cell degeneration and necrosis, and heart interstitial inflammatory infiltrates, including monocytes, lymphocytes, and neutrophils. [157] The cytokine storm observed in COVID-19 patients may originate from multiple sources. Reduced ACE2 expression and increased Ang II accumulation following SARS-CoV-2 infection augment the ACE2/Ang II/AT1R pathway, a mechanism that can promote the cytokine storm, particularly at advanced stages of severe illness, which is characterized by multiple organ failure [ Figure 7A ]. [264] As previously discussed, endocytosis or membrane fusion of SARS-CoV-2 with target cells such as cardiomyocytes, ECs, and cardiac pericytes leads to cell damage or apoptosis, which activates immune responses and promotes the release of inflammatory cytokines [ Figure 7B ]. [111, [253] [254] [255] ECs produce a wide variety of cytokines and chemokines and have been identified as central regulators of systemic inflammatory responses or the cytokine storm that cause loss of vascular barrier integrity and promote pulmonary edema, endothelialitis, and activation of the coagulation pathway. [265] The marked increase in the levels of inflammatory markers such as IL-2R, IL-6, CRP, and TNF-a links with mortality and promotes inter-endothelial gaps and vascular hyperpermeability, [266, 267] a hallmark of the inflammatory response. Cytokines, in turn, activate ECs and increase adhesion molecule expression, [255] leading to vascular disturbances, including leukocyte tethering to the vascular bed, platelet aggregation, and coagulation derangements [ Figure 7B ]. SARS-CoV-2 infection of alveolar epithelial cells and macrophages leads to the release of pathogen-associated molecular patterns and damage-associated molecular patterns, which also activate chemokine (such as CCLs) and pro-inflammatory cytokine (such as IL-1b, IL-6, TNF-a, IFNg) production, contributing to mononuclear cell and lymphocyte recruitment and accumulation [268, 269] and further cytokine production by leukocytes [ Figure 7C ]. [270] When epithelial cells are infected, they release cytokines (IL-1b, IFN-a/b) that can stimulate NK cells. Activated NK cells release cytokines to activate macrophages, which, in turn, release their own cytokines (TNF-a and IL-12), thereby activating NK cells and leading to the formation of a positive feedback cycle [ Figure 7C ]. [271] Viral infection is followed by antigen presentation by lymphocytes and adaptive immune system activation. T helper type 1 (Th1) cells release large amounts of IL-12 and IFN-g for self-stimulation and division, and also activate macrophages that, in turn, activate the innate immune system, which represents an additional feedback mechanism [ Figure 7D ]. [272] A cytokine storm can decrease atherosclerotic plaque stability and favor plaque rupture, microthrombosis, and cardiac injury. [273, 274] The levels of markers of inflammation such as IL-1, IL-6, TNF-a, CRP, erythrocyte sedimentation rate, and fibrinogen are significantly elevated in COVID-19 patients who develop cardiac complications or those in ICUs. [19, 213] In a 3center study from Paris, France, biomarkers at baseline and 60day mortality were analyzed in 101 COVID-19 patients admitted to the ICU. Of these, 83 (83%) were under mechanical ventilation. Although the baseline IL-1b level was undetectable, those of IL-6 and CRP were significantly higher in patients with worsening organ failure than in patients in the non-worsening group. Baseline IL-6 and CRP levels were also significantly higher in non-survivors than in survivors. The levels of IL-6 were positively correlated with organ failure severity. After adjusting for the SOFA score and time from symptom onset to blood sampling, IL-6 and CRP levels were also significantly associated with mortality. [275] Cytokine storm changed blood inflammatory cell profiles in COVID-19 patients and/or blood inflammatory cell profile changes following SARS-CoV-2 infection contributed to the cytokine storm. Histological analysis of the lungs of COVID-19 patients showed the presence of greater numbers of CD4 + T cells but fewer CD8 + T cells compared with lungs from patients with influenza. [201] Flow cytometric analysis of peripheral blood from a patient who died from severe COVID-19 indicated low total numbers of CD4 + and CD8 + T cells; however, these cells were hyperactivated, as evidenced by the high expression of HLA-DR (CD4 + ) and CD38 (CD8 + ). This observation, combined with an increase in the concentrations of pro-inflammatory CCR4 + CCR6 + Th17 cells, [157] was suggestive of severe immune injury. A study from 4 hospitals in China investigated 258 hypertensive and COVID-19 patients, 207 of whom used antiviral agents or steroids. Of the 51 patients who did not use antiviral agents or steroids, 34 had mild disease and 17 had severe disease. Blood levels of CD4 + and CD8 + T cells decreased in the order of normal>mild>severe but cured>severe>died during a 4-week monitoring period. The levels of blood CD38 + HLA-DR + and CD38 + PD-1 + CD8 + T cells, IFNG + CD8 + T cells, and IFNG + CD4 + T cells were also elevated among patients who survived, as well as those who died during the 4 weeks following symptom onset [ Figure 7 ]. [276] As expected, the concentrations of SARS-CoV-2-specific IgG, IgM, and IgA antibodies were higher in survivors than in non-survivors. [276] CD14 dim CD16 + nonclassical monocytes mediate anti-viral immune responses and play athero-protective, anti-inflammatory, and pro-homoeostatic roles [ Figure 7 ]. [277, 278] In a cohort of 96 consecutive patients from Tübingen, Germany, including 47 who had CAD and COVID-19, 19 who had CAD only, and 30 who were healthy, the numbers of these non-classical monocytes in the blood were markedly lower in CAD-COVID-19 patients than in those of the other 2 groups (P < 0.0001), and these cells showed decreased expression of adhesion, migration, and T-cell activation markers (CD54, CD62L, CX3CR1, CD80, and HLA-DR). Decreased numbers of these cells were positively associated with ICU admission, respiratory failure, and use of mechanical ventilation. [279] Impact of SARS-CoV-2 infection on common CVD therapy ACE2 is required for SARS-CoV-2 entry into host cells. Increased ACE2 expression likely heightens the risk of CVD and increases the likelihood of severe SARS-CoV-2 infection. Nevertheless, myocardium ACE2 expression is decreased in post-mortem autopsy samples from SARS-CoV-2-infected patients, and this reduction is associated with a concomitant increase in myocardial inflammation and fibrosis. [111, [253] [254] [255] Increased ACE2 expression protects against Ang II-induced vasoconstriction and inflammation but also increases susceptibility to SARS-CoV-2 infection. In particular, the therapeutic RAS blockade agents used in CVD management, such as ACE inhibitors (ACEIs) and angiotensin receptor blockers (ARBs), increase ACE2 expression, thereby increasing the susceptibility to SARS-CoV-2 infection and the pathogenicity of COVID-19. For instance, the ARBs losartan and olmesartan increase ACE2 expression in rat hearts after MI, [280] while enalapril normalizes ACE2 expression in rat LVs following HF. [281] These observations suggest that an increase in ACE2 levels may be protective against SARS-CoV-2 infection due to the increase in Ang II catabolism and Ang-(1-7) production [ Figure 2 ]. [282] However, to date, there is a lack of consistent results to support that RAS blockers improve prognosis, [78] or that ACEIs and ARBs affect the susceptibility to SARS-CoV-2 infection. [283] Contradictory results were obtained from both experimental models and patient population studies. Anti-hypertensive treatments with ACEIs or ARBs (lisinopril, losartan, enalapril) were associated with increased ACE2 levels in the LVs of normotensive rats. [14, 151] Results from a single-center retrospective study from Wuhan, China, showed that ACEIs and ARBs reduced the blood levels of inflammatory biomarkers (CRP and procalcitonin) and yielded better outcomes in 43 COVID-19 patients compared with those of 83 patients treated with other anti-hypertensive drugs. [284] A retrospective multicenter study of 1128 adult COVID-19 patients with hypertension from 9 hospitals in Wuhan, China, showed that the use of these ACEIs and ARBs was associated with a 58% reduction in the risk for all-cause death (adjHR = 0.42) after adjusting for age, gender, comorbidities, and in-hospital medications. [285] The French National Health Insurance database of almost 2 million hypertensive patients assessed whether the use of anti-hypertensive drugs affected the risk for COVID-19. ACEIs and ARBs were compared with calcium channel blockers. Three exclusive cohorts that included individuals aged 18 to 80 were followed from February 15 to June 7, 2020. Endpoints were time to hospitalization for COVID-19 and time to intubation/death during hospital stay. Within 16 weeks, 2338 individuals were hospitalized, while 526 died or were intubated for COVID-19. ACEIs and ARBs lowered the risk of COVID-19related hospitalization in both males and females and in all age groups (from 18 to 80). ACEIs and ARBs also lowered the risk of death/intubation in males and females, as well as in patients between the ages of 51 and 80, but not in those aged 18 to 50. [286] Analysis of the Healthcare database from the Health Insurance Review and Assessment Service of Korea, which covers the entire Korean population of 50 million people from January 1, 2015, to April 8, 2020 , showed that 69,793 individuals underwent COVID-19 screening. Among 1290 hypertensive and COVID-19-positive patients, 682 had records of ACEI/ARB use while 603 did not. Inverse probability of treatment weighting was used to mitigate selection bias and a Poisson regression model was used to estimate the relative risks to compare the outcomes between ACEI/ARB users and non-users. The use of ACEIs/ARBs was found to be associated with better clinical outcomes (adjRR = 0.60, P = 0.005) and showed a protective effect in overall outcomes in both men (adjRR = 0.84, P = 0.008) and patients with pre-existing respiratory disease (adjRR = 0.74, P = 0 .002); however, no association was found between ACEI/ARB use and all-cause mortality (adjRR = 0.62, P = 0.097) or respiratory events (adjRR = 0.99, P = 0.84). [287] Two clinical trials of losartan as an additional treatment for COVID-19 are currently underway (NCT04311177 and NCT043120-09).

Different population studies have also shown that RAS inhibitors neither increase the risk of SARS-CoV-2 infection in patients with hypertension nor negatively influence disease severity. A case-population study of 1139 COVID-19 patients and 11,390 controls showed that RAS inhibitors do not increase the risk of COVID-19-related hospital admission when compared with other anti-hypertensive drugs (OR = 0.94). [288] A randomized clinical trial of 659 patients hospitalized in Brazil with mild (57.1%) to moderate (42.9%) COVID-19 were grouped 1:1 with or without ACEIs or ARBs for 30 days. The primary outcome was days alive and out of hospital during this 30-day period. No difference was found between patients taking the ACEIs or ARBs and those who did not. Additionally, no differences were identified in the mortality rate, cardiovascular death, or COVID-19 progression. [289] Studies of 3179 patients who had been discharged or had died selected from 5625 COVID-19 inpatients from 61 hospitals across Italy showed that the use of ACEIs, ARBs, or Ca-antagonists was not associated with risk of death. Only a marginal negative association was found between ARB use and risk of death (adjOR = 0.65, P = 0.025), while a marginal positive association was found between diuretic use and risk of death (OR = 1.66, P = 0.020). [290] At the very least, these studies established the safety of ACEIs and ARBs, [70, 71, 78, 291] and support the continued use of ACEIs and ARBs in patients with COVID-19, although the debate continues. [292] [293] [294] [295] Glucocorticoids are not recommended for treating SARS-CoV-2-induced lung injury based on the results of a UK-based study. Although glucocorticoids inhibit lung inflammation, they also increase the risk of CVD events. [296] Studies from SARS-CoV showed that glucocorticoids also inhibit the immune response and virus elimination. [297] In a UK community-based biobank cohort of 473,555 individuals that contained 459 COVID-19 deaths and 2626 non-COVID-19 deaths, besides age, sex, being Black, and comorbidities, the use of oral steroids, but not ACEIs or ARBs, was associated with an increased risk of COVID-19related death. [49] However, several clinical studies have yielded the opposite conclusions. In Italy, a study of 692 consecutive patients with COVID-19, including 90 (13.0%) who had a history of HF, from 13 Italian cardiology centers between March 1 and April 9, 2020, showed that in-hospital treatment with corticosteroids and heparin had beneficial effects. Adjusted HRs for death were 0.46 (P = 0.001, n = 404) for corticosteroid treatment and 0.41 (P < 0.001, n = 364) for heparin treatment. [77] Patient background, corticosteroid dose, and treatment length may be associated with these discrepancies. In the RECOVERY trial of hospitalized COVID-19 patients from the UK that included 2140 patients who received oral or intravenous lowdose, short-term dexamethasone treatment (6 mg/day for 10 days) and 4321 who received standard care, the 28-day fatality rate was significantly reduced in those receiving dexamethasone (adjRR = 0.83, P < 0.001). The incidence of death was lower in the dexamethasone group than in the standard care group among patients receiving invasive ventilation (adjRR = 0.64) and those receiving oxygen, but not those receiving ventilation (adjRR = 0.82). [298] Compared with standard care alone, higher dose, intravenous dexamethasone treatment (20 mg daily for 5 days or 10 mg daily for 5 days plus standard care) in 151 COVID-19 patients with moderate to severe ARDS from Brazil increased the number of ventilation-free days during the first 28 days (n = 148, P = 0.04). [299] A study of 86 hospitalized COVID-19 patients from Iran showed similar results. Low doses of methylprednisolone (2 mg/day) or dexamethasone (6 mg/day) significantly improved clinical status at day 5 (P = 0.002) and day 10 (P = 0.001) and reduced the overall disease score (3.909 vs. 4.873, P = 0.004) and length of hospital stay (7.43 days vs. 10.52 days, P = 0.015). [300] In a similar randomized controlled trial involving 68 hospitalized COVID-19 patients from Iran, patients receiving high doses of intravenous methylprednisolone (n = 34, 250 mg/ day) for 3 days showed a markedly higher percentage of recovery (94.1% vs. 57.1%, P < 0.001), a lower mortality rate (5.9% vs. 42.9%, P < 0.001), and longer survival time (HR = 0.293, logrank test P < 0.001) compared with those receiving standard care (n = 34). [301] Several meta-analyses indicated that corticosteroids remain effective and safe among severely ill COVID-19 patients, but not among all hospitalized COVID-19 patients. [302] [303] [304] Accordingly, the WHO guidelines recommend corticosteroid administration for patients with severe COVID-19 or those critically ill with the disease, but not for patients with non-severe COVID-19. [305] A recent study from Wuhan, China, assessed the effect of lowdose aspirin on COVID-19 patients who also had CAD. Among 183 patients, 52 took low-dose aspirin and 131 did not. Multivariate analysis indicated that aspirin use did not affect either in-hospital mortality or all-cause mortality. [306] However, some studies have reported different results. Among 28 COVID-19 patients who received aspirin and 204 who did not, those receiving aspirin were significantly older and were more likely to be hypertensive and suffer from cerebrovascular disease and coronary disease (all P < 0.001). Both the 30-day (4.17% vs. 29.2%) and 60-day (8.3% vs. 33.3%) mortality rates were markedly lower in patients of the low-dose aspirin group than in those of the non-aspirin group. [307] In a very recent study of 412 COVID-19 patients from a multicenter (Collaborative Research to Understand the Sequelae of Harm in COVID (CRUSH COVID)) registry from Maryland, USA, 98 patients received aspirin 24 hours or 7 days before admission. Again, aspirin users were significantly older and had a greater probability of suffering from hypertension, diabetes, CAD, renal disease, and betablocker use (all P < 0.001). Aspirin use significantly reduced the risk of mechanical ventilation use (HR = 0.56, P = 0.007), ICU admission (HR = 0.57, P = 0.005), and in-hospital mortality (HR = 0.53, P = 0.02). [308] Aspirin has triple effects, namely, it inhibits virus replication and has both anticoagulant and antiinflammatory properties. Aspirin was suggested to exert protective effects in COVID-19 patients, although the results from a clinical trial (NCT04365309) from Xijing Hospital, Xi'an, China, that was completed in June 2020 have not been released.

Statins and proprotein convertase subtilisin/kexin type 9 (PCSK9) inhibitors are both commonly used among CVD patients, but these lipid-lowering drugs have also proved beneficial for COVID-19 patients. Multiple studies have reported that COVID-19 patients have low blood levels of low-density (LDL) and high-density (HDL) lipoproteins. LDL and HDL levels were even lower in patients with severe disease or deceased patients. [309] [310] [311] [312] In contrast, blood triglyceride levels are significantly elevated in COVID-19 patients. From 98 COVID-19 patients from Wuhan, China, including 46 who were admitted to the ICU, 32 and 46 who suffered from myocardial injury and HF, respectively, and 36 who died, the blood triglyceride to HDL-C ratio was increased in patients with myocardial injury, HF, severe illness, and fatal outcome, while the baseline triglyceride to HDL-C ratio was correlated with the levels of hs-cTnI (r = 0.251, P = 0.018), NT-proBNP (r = 0.274, P = 0.008), HbA1C (r = 0.239, P = 0.038), and IL-6 (r = 0.218, P = 0.042). Multivariate logistic regression analysis showed that an increased triglyceride to HDL-C ratio was an independent risk factor for myocardial injury (OR = 2.73, P = 0.013), HF (OR = 2.64, P = 0.019), severe illness (OR = 3.01, P = 0.032), and fatal outcome (OR = 2.97, P = 0.014). [313] A retrospective chart review of 139 COVID-19 patients, including 26 deceased and 113 survivors, showed that deceased patients were older (P < 0.001), were more likely to suffer from hypertension (P < 0.006), diabetes mellitus (P = 0.002), CVD (P = 0.001), chronic renal insufficiency (P = 0.003), and atrial fibrillation (P = 0.003). Deceased patients also had lower plasma total cholesterol (P = 0.004), HDL-C (P < 0.001), and LDL-C (P < 0.001) levels but higher triglyceride levels (P < 0.001), compared with those of survivors. [314] Statin use decreased COVID-19 patient fatality and severity by nearly 30%. [315] In a study of 154 elderly nursing home residents with COVID-19, a history of statin use was associated with an absence of symptoms during COVID-19 infection (OR = 2.65, P = 0.028), even after adjusting for age, sex, and comorbidities such as diabetes, hypertension, and functional status. [316] Another study enrolled 13,981 patients with COVID-19, 1219 of whom received statins. The 28-day all-cause of mortality in a propensity score-matching model was 5.2% and 9.4% among statin and non-statin users (adjHR = 0.58, P = 0.001), respectively. Statin therapy was also associated with lower blood CRP and IL-6 concentrations and neutrophil counts. [317] A meta-analysis that included 4 studies and 8990 COVID-19 patients revealed that statin use was associated with a significantly reduced risk of COVID-19-associated death and disease severity (HR = 0.70, P = 0.01) compared with non-use of statins. [315] These results suggest that statin treatment may protect against coronary endothelial dysfunction due to COVID-19.

Statins and PCSK9 inhibitors may mitigate SARS-CoV-2 infection through multiple mechanisms. Statins reduce inflammation and oxidative stress. [318, 319] They inhibit the rate-limiting enzyme of the mevalonate pathway, leading to reduced GTPase activity, which is essential for cell migration, activation, signaling, and cytokine expression. [320] Statins stabilize MyD88, suppress TLR function, and attenuate NF-kB activation following a proinflammatory trigger (eg, hypoxia). [320, 321] Statins also reduce ox-LDL levels and NADPH oxidase activity, which decreases ROS generation by inhibiting NF-kB signaling pathway-associated transcription or by improving endothelial NO synthase coupling. [322] Like ACEIs and ARBs, [323] statin treatment also increases ACE2 expression. [324] Statins may exert similar antiviral activity to that of ACEIs and ARBs by reducing Ang II availability and increasing Ang-(1-7) production. Statin-or PCSK9 inhibitor-mediated lipid reduction may block SARS-CoV-2 infection by impeding its entry into host cells through the disruption of lipid rafts. Statins, such as pitavastatin, could also reduce SARS-CoV-2 infectivity by inhibiting its main protease, which plays a role in proteolytic maturation and viral replication. [325] Accordingly, statins and PCSK9 inhibitors may act as immunomodulators in COVID-19 because of their antioxidative, anti-arrhythmic, and anti-thrombotic properties. Their use is associated with improved endothelial functions, reduced oxidative stress, less platelet adhesion, and increased atherosclerotic plaque stability. [319, 326] Thus, the use of lipid-lowering drugs may not need to be discontinued in COVID-19 patients with CVD. [327] 

Due to the rapidly evolving basic and clinical research relating to COVID-19, the contents of this manuscript are subject to modification as we learn more about this devastating disease. Given the broad tissue distribution of ACE2 in many tested organs, it seems likely that SARS-CoV-2 infects not only the lungs and heart but also many other organs that are not discussed in this review. Furthermore, many of the recently published studies are still observational and descriptive, even though this disease was first documented more than a year ago. Our knowledge about SARS-CoV-2 remains incomplete, especially the continuing emergence of different mutants. Despite this, we hope that the contents presented here will aid in our understanding of the disease, particularly the interconnection between COVID-19 and CVD, drug selection, or perhaps most importantly, patient care.

The authors thank Ms. Chelsea Swallom for her editorial assistance.

Dr. Shi receives funding support from the National Heart, Lung, and Blood Institute (R01HL151627) and the National Institute of Neurological Disorders and Stroke (R01AG063839). Dr. Libby receives funding support from the National Heart, Lung, and Blood Institute (1R01HL134892), the American Heart Association (18CSA34080399), and the RRM Charitable Fund, and the Simard Fund. 

Dr. Libby is an unpaid consultant to, or involved in clinical trials for Amgen, AstraZeneca, Baim Institute, Beren Therapeutics, Esperion Therapeutics, Genentech, Kancera, Kowa Pharmaceuticals, Medimmune, Merck, Norvo Nordisk, Novartis, Pfizer, and Sanofi-Regeneron. Dr. Libby is a member of the scientific advisory board for Amgen, Caristo, Cartesian, CSL Behring, DalCor Pharmaceuticals, Dewpoint, Kowa Pharmaceuticals, Olatec Therapeutics, Medimmune, Novartis, PlaqueTec, and XBiotech, Inc.

Dr. Libby's laboratory has received research funding in the last 2 years from Novartis. Dr. Libby is on the Board of Directors of XBiotech, Inc. Dr. Libby has a financial interest in Xbiotech, a company developing therapeutic human antibodies. Dr. Libby's interests were reviewed and are managed by Brigham and Women's Hospital and Partners HealthCare in accordance with their conflict-of-interest policies.

Editor note: Peter Libby is an Associate Editor of Cardiology Discovery. Guo-Ping Shi is an Editorial Board Member of Cardiology Discovery. The article was subject to the journal's standard procedures, with peer review handled independently of these editors and their research groups.

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SARS-CoV-2 in cardiac tissue of a child with COVID-19-related multisystem inflammatory syndrome

Evidence of SARS-CoV-2 transcriptional activity in cardiomyocytes of COVID-19 patients without clinical signs of cardiac involvement

Human iPSC-derived cardiomyocytes are susceptible to SARS-CoV-2 infection

Clinical and biochemical indexes from 2019-nCoV infected patients linked to viral loads and lung injury

Altered blood pressure responses and normal cardiac phenotype in ACE2-null mice

Overexpression of angiotensin-converting enzyme 2 in the rostral ventrolateral medulla causes long-term decrease in blood pressure in the spontaneously hypertensive rats

Transgenic angiotensinconverting enzyme 2 overexpression in vessels of SHRSP rats reduces blood pressure and improves endothelial function

Angiotensin-converting enzyme 2 is an essential regulator of heart function

Altered heart rate and blood pressure variability in mice lacking the Mas protooncogene

Role of the ACE2/angiotensin 1-7 axis of the renin-angiotensin system in heart failure

Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China

Hyperglycemia induces neutrophil extracellular traps formation through an NADPH oxidase-dependent pathway in diabetic retinopathy

Targeting potential drivers of COVID-19: neutrophil extracellular traps

Prevalence and impact of diabetes among people infected with SARS-CoV-2

Clinical perspective of hypoxia-mediated pulmonary hypertension

Oxygen, oxidative stress, hypoxia, and heart failure

Pathological findings of COVID-19 associated with acute respiratory distress syndrome

Recognizing COVID-19-related myocarditis: the possible pathophysiology and proposed guideline for diagnosis and management

SARS-Cov-2 fulminant myocarditis: an autopsy and histopathological case study

Early detection of elevated cardiac biomarkers to optimise risk stratification in patients with COVID-19

Cardiology Discovery (2021) 1 4 www.cardio-discovery.org

A pneumonia outbreak associated with a new coronavirus of probable bat origin

Cardiac injury is associated with inflammation in geriatric COVID-19 patients

Analysis of myocardial injury in patients with COVID-19 and association between concomitant cardiovascular diseases and severity of COVID-19

Characterization of myocardial injury in patients with COVID-19

Myocardial injury in adults hospitalized with COVID-19

Left and right ventricular dysfunction in patients with COVID-19-associated myocardial injury

Cardiac injury is associated with mortality and critically ill pneumonia in COVID-19: a metaanalysis

Cardiovascular manifestations and treatment considerations in COVID-19

Myocardial localization of coronavirus in COVID-19 cardiogenic shock

Association of cardiac infection with SARS-CoV-2 in confirmed COVID-19 autopsy cases

Factors associated with myocardial SARS-CoV-2 infection, myocarditis, and cardiac inflammation in patients with COVID-19

Pulmonary and cardiac pathology in African American patients with COVID-19: an autopsy series from New Orleans

T-cell immunity in myocardial inflammation: pathogenic role and therapeutic manipulation

COVID-19 and the heart

D-dimer levels on admission to predict in-hospital mortality in patients with Covid-19

Reduction in ST-segment elevation cardiac catheterization laboratory activations in the United States during COVID-19 pandemic

The obstacle course of reperfusion for ST-segment-elevation myocardial infarction in the COVID-19 pandemic

Papillary muscle rupture due to delayed STEMI presentation in a patient self-isolating for presumed COVID-19

European Society for Cardiology. The European Society for Cardiology (2020) ESC guidance for the diagnosis and management of CV disease during the COVID-19 pandemic

Clinical characteristics of 113 deceased patients with coronavirus disease 2019: retrospective study

Network-based analysis of comorbidities risk during an infection: SARS and HIV case studies

Clinical and laboratory features of severe acute respiratory syndrome vis-a-vis onset of fever

Prognostic value of NT-proBNP in patients with severe COVID-19

Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study

Prognostic impact of prior heart failure in patients hospitalized with COVID-19

Inflammation -cause or consequence of heart failure or both

Continuous glycoprotein-130-mediated signal transducer and activator of transcription-3 activation promotes inflammation, left ventricular rupture, and adverse outcome in subacute myocardial infarction

Interleukin-6 receptor inhibition modulates the immune reaction and restores titin phosphorylation in experimental myocarditis

Interleukin-1 beta modulates the growth and phenotype of neonatal rat cardiac myocytes

Cellular basis for the negative inotropic effects of tumor necrosis factor-alpha in the adult mammalian heart

TNF-alpha signal transduction in rat neonatal cardiac myocytes: definition of pathways generating from the TNF-alpha receptor

IL-1beta and TNF-alpha upregulate angiotensin II type 1 (AT1) receptors on cardiac fibroblasts and are associated with increased AT1 density in the post-MI heart

TNF-a increases cardiac fibroblast lysyl oxidase expression through TGF-b and PI3Kinase signaling pathways

COVID-19 and cardiac arrhythmias

COVID-19 gone bad: a new character in the spectrum of the hyperferritinemic syndrome

Clinical characteristics of novel coronavirus cases in tertiary hospitals in Hubei Province

Clinical features and short-term outcomes of 18 patients with corona virus disease 2019 in intensive care unit

Predictors and prognostic implications of cardiac arrhythmias in patients hospitalized for COVID-19

Worldwide survey of COVID-19-associated arrhythmias

Pulmonary vascular endothelialitis, thrombosis, and angiogenesis in Covid-19

Cardiac involvement in a patient with coronavirus disease 2019 (COVID-19)

Therapeutic Anticoagulation Is Associated with Decreased Mortality in Mechanically Ventilated COVID-19 Patients

Increased serum levels of hepcidin and ferritin are associated with severity of COVID-19

Hepcidin levels predict Covid-19 severity and mortality in a cohort of hospitalized Italian patients

Interleukin-6 is a biomarker for the development of fatal severe acute respiratory syndrome coronavirus 2 pneumonia

Severe arterial thrombosis associated with Covid-19 infection

Coronavirus disease 2019, prothrombotic factors, and venous thromboembolism

Characteristics of ischaemic stroke associated with COVID-19

Severe acute respiratory syndrome coronavirus 2 infection and ischemic stroke

Clinical spectrum of ischaemic arterial diseases associated with COVID-19: a series of four illustrative cases

Abnormal coagulation parameters are associated with poor prognosis in patients with novel coronavirus pneumonia

COVID-19 and its implications for thrombosis and anticoagulation

Incidence of thrombotic complications in critically ill ICU patients with COVID-19

Hypercoagulability of COVID-19 patients in intensive care unit: a report of thromboelastography findings and other parameters of hemostasis

The procoagulant pattern of patients with COVID-19 acute respiratory distress syndrome

Fibrinolysis shutdown and thrombosis in severe COVID-19

D-dimer as a prognostic indicator in critically ill patients hospitalized with COVID-19 in Leishenshan Hospital

High risk of thrombosis in patients with severe SARS-CoV-2 infection: a multicenter prospective cohort study

Thrombosis and platelets: an update

Platelet gene expression and function in patients with COVID-19

Platelets can associate with SARS-Cov-2 RNA and are hyperactivated in COVID-19

Potential role for tissue factor in the pathogenesis of hypercoagulability associated with in COVID-19

Platelet activation and platelet-monocyte aggregate formation trigger tissue factor expression in patients with severe COVID-19

Thrombosis as an intravascular effector of innate immunity

Association of neutrophil activation, more than platelet activation, with thrombotic complications in coronavirus disease 2019

Thrombocytopenia is associated with severe coronavirus disease 2019 (COVID-19) infections: A metaanalysis

Thrombocytopenia and its association with mortality in patients with COVID-19

What do monitoring platelet counts in COVID-19 teach us?

Exploring possible mechanisms for COVID-19 induced thrombocytopenia: unanswered questions

Heart disease in patients with pulmonary embolism

Pulmonary embolism as a cause of cardiac arrest: presentation and outcome

A prospective study on cardiovascular events after acute pulmonary embolism

SARS-CoV-2 and cardiovascular complications: from molecular mechanisms to pharmaceutical management

Delay of acute intracellular pH recovery after acidosis decreases endothelial cell activation

High thrombus burden in patients with COVID-19 presenting with ST-segment elevation myocardial infarction

Acute pulmonary embolism and COVID-19 pneumonia: a random association

Thromboembolic risk and anticoagulant therapy in COVID-19 patients: emerging evidence and call for action

Prevalence of venous thromboembolism in patients with severe novel coronavirus pneumonia

Confirmation of the high cumulative incidence of thrombotic complications in critically ill ICU patients with COVID-19: An updated analysis

Pulmonary embolism in patients with COVID-19: awareness of an increased prevalence

Incidence of venous thromboembolism in hospitalized patients with COVID-19

COVID-19 and venous thromboembolism: a meta-analysis of literature studies

Risk of stroke in hospitalized SARS-CoV-2 infected patients: a multinational study

Thrombosis in hospitalized patients with COVID-19 in a New York City Health System

Detectable 2019-nCoV viral RNA in blood is a strong indicator for the further clinical severity

Cardiology Discovery (2021) 1 4 www.cardio-discovery.org

Mediators of SARS-CoV-2 entry are preferentially enriched in cardiomyocytes

The cardiovascular burden of coronavirus disease 2019 (COVID-19) with a focus on congenital heart disease

Inhibition of SARS-CoV-2 infections in engineered human tissues using clinical-grade soluble human ACE2

SARS-CoV-2 leads to a small vessel endotheliitis in the heart

Endothelial cell infection and endotheliitis in COVID-19

Endotheliitis and endothelial dysfunction in patients with COVID-19: its role in thrombosis and adverse outcomes

Redox biology of respiratory viral infections

Angiotensin (1-7) and other angiotensin peptides

The science underlying COVID-19: implications for the cardiovascular system

COVID-19: the vasculature unleashed

Oxidative stress: harms and benefits for human health

Endothelial cell dysfunction: a major player in SARS-CoV-2 infection (COVID-19)

Endothelin and endothelial dysfunction

COVID-19, renin-angiotensin system and endothelial

The vascular endothelium: the cornerstone of organ dysfunction in severe SARS-CoV-2 infection

Endotheliopathy in COVID-19-associated coagulopathy: evidence from a single-centre, crosssectional study

The SARS-CoV-2 spike protein disrupts the cooperative function of human cardiac pericytesendothelial cells through CD147 receptor-mediated signalling: a potential non-infective mechanism of COVID-19 microvascular disease

COVID-19 illness in native and immunosuppressed states: a clinical-therapeutic staging proposal

Cardiovascular disease and COVID-19

COVID-19 cytokine storm: the interplay between inflammation and coagulation

Inflammatory cytokines in the BAL of patients with ARDS. Persistent elevation over time predicts poor outcome

The trinity of COVID-19: immunity, inflammation and intervention

The pathogenesis and treatment of the 'Cytokine Storm' in COVID-19

Review: cytokine storm syndrome: looking toward the precision medicine era

Developmental and functional control of natural killer cells by cytokines

Cytokine release syndrome

Impact of coronavirus disease 2019 on pulmonary function in early convalescence phase

Coronaviruses and the cardiovascular system: acute and long-term implications

Elevated plasma IL-6 and CRP levels are associated with adverse clinical outcomes and death in critically ill SARS-CoV-2 patients: inflammatory response of SARS-CoV-2 patients

Dynamic SARS-CoV-2-specific immunity in critically ill patients with hypertension

Nonclassical monocytes in health and disease

Human CD14dim monocytes patrol and sense nucleic acids and viruses via TLR7 and TLR8 receptors

Numbers and phenotype of non-classical CD14dimCD16+ monocytes are predictors of adverse clinical outcome in patients with coronary artery disease and severe SARS-CoV-2 infection

Upregulation of angiotensin-converting enzyme 2 after myocardial infarction by blockade of angiotensin II receptors

Enalapril attenuates downregulation of Angiotensin-converting enzyme 2 in the late phase of ventricular dysfunction in myocardial infarcted rat

Renin-angiotensinaldosterone system inhibitors in patients with Covid-19

Cardiovascular disease, drug therapy, and mortality in Covid-19

Effects of angiotensin II receptor blockers and ACE (angiotensin-converting enzyme) inhibitors on virus infection, inflammatory status, and clinical outcomes in patients with COVID-19 and hypertension: a single-center retrospective study

Association of inpatient use of angiotensin-converting enzyme inhibitors and angiotensin II receptor blockers with mortality among patients with hypertension hospitalized with COVID-19

Antihypertensive drugs and COVID-19 risk: a cohort study of 2 million hypertensive patients

Clinical outcomes of COVID-19 following the use of angiotensin-converting enzyme inhibitors or angiotensin-receptor blockers among patients with hypertension in Korea: a nationwide study

Use of reninangiotensin-aldosterone system inhibitors and risk of COVID-19 requiring admission to hospital: a case-population study

Effect of discontinuing vs continuing angiotensin-converting enzyme inhibitors and angiotensin II receptor blockers on days alive and out of the hospital in patients admitted with COVID-19: a randomized clinical trial

Comorbidities, cardiovascular therapies, and COVID-19 mortality: a nationwide, Italian Observational Study (ItaliCO)

Association of angiotensinconverting enzyme inhibitor or angiotensin receptor blocker use with COVID-19 diagnosis and mortality

RAAS inhibitors and risk of Covid-19

RAAS inhibitors and risk of Covid-19

RAAS inhibitors and risk of Covid-19. Reply

RAAS inhibitors and risk of Covid-19. Reply

Dose-dependent oral glucocorticoid cardiovascular risks in people with immune-mediated inflammatory diseases: a population-based cohort study

Clinical evidence does not support corticosteroid treatment for 2019-nCoV lung injury

Dexamethasone in hospitalized patients with Covid-19

Effect of dexamethasone on days alive and ventilator-free in patients with moderate or severe acute respiratory distress syndrome and COVID-19: the CoDEX randomized clinical trial

Methylprednisolone or dexamethasone, which one is superior corticosteroid in the treatment of hospitalized COVID-19 patients: a triple-blinded randomized controlled trial

Intravenous methylprednisolone pulse as a treatment for hospitalised severe COVID-19 patients: results from a randomised controlled clinical trial

Efficacy and safety of systematic corticosteroids among severe COVID-19 patients: a systematic review and meta-analysis of randomized controlled trials

Impact of corticosteroids in coronavirus disease 2019 outcomes: systematic review and meta-analysis

Impact of systemic corticosteroids on hospitalized patients with COVID-19

A living WHO guideline on drugs for covid-19

Mortality and pre-hospitalization use of low-dose aspirin in COVID-19 patients with coronary artery disease

Effect of low-dose aspirin on mortality and viral duration of the hospitalized adults with COVID-19

Aspirin use is associated with decreased mechanical ventilation, intensive care unit admission, and in-hospital mortality in hospitalized patients with coronavirus disease 2019

Hypolipidemia is associated with the severity of COVID-19

Low high-density lipoprotein level is correlated with the severity of COVID-19 patients: an observational study

Declined serum high density lipoprotein cholesterol is associated with the severity of COVID-19 infection

Lipoprotein concentrations over time in the intensive care unit COVID-19 patients: Results from the ApoCOVID study

Triglyceride to high-density lipoprotein cholesterol ratio is an important determinant of cardiovascular risk and poor prognosis in coronavirus disease-19: a retrospective case series study

The atherogenic index of plasma as a predictor of mortality in patients with COVID-19

Meta-analysis of effect of statins in patients with COVID-19

The effects of ARBs, ACEis, and statins on clinical outcomes of COVID-19 infection among nursing home residents

In-hospital use of statins is associated with a reduced risk of mortality among individuals with COVID-19

Immune modulatory effects of statins

Biology of proprotein convertase subtilisin kexin 9: beyond low-density lipoprotein cholesterol lowering

Anti-inflammatory action of statins in cardiovascular disease: the role of inflammasome and toll-like receptor pathways

Atorvastatin attenuates myocardial remodeling induced by chronic intermittent hypoxia in rats: partly involvement of TLR-4/MYD88 pathway

Endothelial dysfunction in COVID-19: current findings and therapeutic implications

Angiotensin converting enzyme inhibitors and angiotensin receptor blockers and mortality among COVID-19 patients: a systematic review and meta-analysis

Online ahead of print

Statin therapy in COVID-19 infection

Statins and the COVID-19 main protease: in silico evidence on direct interaction

Statins and cardioprotection -more than just lipid lowering

Statins and PCSK9 inhibitors: what is their role in coronavirus disease 2019

COVID-19, the Pandemic of the Century and Its Impact on Cardiovascular Diseases