key: cord-0975736-ox1i4avh authors: Gan, Samuel Ken-En; Phua, Ser-Xian; Yeo, Joshua Yi title: Sagacious epitope selection for vaccines, and both antibody-based therapeutics and diagnostics: tips from virology and oncology date: 2022-02-21 journal: Antib Ther DOI: 10.1093/abt/tbac005 sha: 4053087aaad3dd1a65b7c5e06566e1cfc3e40c92 doc_id: 975736 cord_uid: ox1i4avh The target of an antibody plays a significant role in the success of antibody-based therapeutics and diagnostics, and vaccine development. This importance is focused on the target binding site—epitope, where epitope selection as a part of design thinking beyond traditional antigen selection using whole cell or whole protein immunization can positively impact success. With purified recombinant protein production and peptide synthesis to display limited/selected epitopes, intrinsic factors that can affect the functioning of resulting antibodies can be more easily selected for. Many of these factors stem from the location of the epitope that can impact accessibility of the antibody to the epitope at a cellular or molecular level, direct inhibition of target antigen activity, conservation of function despite escape mutations, and even noncompetitive inhibition sites. By incorporating novel computational methods for predicting antigen changes to model-informed drug discovery and development, superior vaccines and antibody-based therapeutics or diagnostics can be easily designed to mitigate failures. With detailed examples, this review highlights the new opportunities, factors, and methods of predicting antigenic changes for consideration in sagacious epitope selection. Antibodies and their fragments are increasingly important in diagnostics and therapeutics development as evidenced in the ongoing COVID-19 pandemic [1, 2] . An already expensive process, diagnostics can fail owing to escape mutations on the epitope that compromise primer based kits [2] [3] [4] or diagnostic antibody binding [5] , even with sagacious rational antibody design and engineering [6] . On therapeutics, antigenic epitope changes leading to escape mutations can contribute to drug failures. Thereby, e.g. phage display for antibody selection. The same technology also supported the targeting of specific epitopes on the antigen, where having antibodies specific to an epitope in a diagnostic kit can improve selectivity and specificity. This specificity is useful when differentiating between similar antigens e.g. between reverse transcriptase (RT) of viruses such as human immunodeficiency virus (HIV) and hepatitis B virus (HBV) or the RNA-dependent RNA polymerase (RdRp) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from Middle East Respiratory Syndrome (MERS), influenza, and severe acute respiratory syndrome (SARS). Yet, being too specific in diagnostics, as opposed for vaccinations, can also result in false negatives when the target epitope on the antigen mutates beyond antibody recognition [11] . With advances in peptide technology, short/stapled peptides can also be used without necessarily going to the deoxyribonucleic acid (DNA) level for recombinant expression and the commonly used whole cells or antigens [12, 13] . Recent developments in cyclic peptides and peptide vaccines further allow for immunization against specific conformational structures in epitopes instead of whole antigens through mimotopes [14] as B cell peptides [15] . Such methods of greater selectivity can support the development of therapeutics to reduce off-target effects, although some level of lower specificity could be of value for vaccines, and to an extent, diagnostics to target variants. The selection of epitopes when incorporated into antibody design thinking is thus a paradigm shift from 'chancedependent' antibody development to a more rational and purposeful approach. Given the dependence on the intended application in guiding toward higher specificity or to cater to minor changes, there are two categories [16] of (1) linear/continuous: defined as a stretch of amino acids sequences; (2) conformational/discontinuous: defined as sequence distal residues in close proximity through protein folding, with the latter conformational type more prevalent as B-cell epitopes. About 96% of monoclonal antibody therapeutic candidates fail to make it to the market [17] , costing close to tens of millions of dollars [18] for each failure, augmenting the MID3 approach that includes epitope prediction. Epitope prediction, traditionally based on amino acid physicochemical properties such as hydrophobicity, flexibility, solvent accessibility, and antigenicity [19] [20] [21] [22] , has seen augmentation by machine learning methods to show promise for cancer [23] and even hybrid experimentalcomputational approaches [24] involving deep neural network for major histocompatibility complex binding [25] and attention-based long short-term memory networks [26] . For epitope selection, particularly for therapeutics, accessibility of the epitope by the antibodies is perhaps the first and foremost consideration. In maximizing success, extracellular targets are typically picked for therapeutic antibody candidates with the exception of 'intrabodies' (where target cells produce the antibodies against intracellular antigens within itself [27] ). Apart from intrabodies, whole antibodies against intracellular targets often have limited penetration of membranes (knowledge known to frequent flow cytometry users) requiring intracellular delivery methods [28, 29] not always feasible for therapeutics. Although unknown in prevalence, there are instances where intracellular oncogenic markers can be targeted because of externalization e.g. PRL3 [30] . Despite the cellular penetration handicap, antibodies have intrinsic advantages for targets unsuitable for small molecules [29] due to specificity requirement. Thus, personalized assessment of targeting intracellular proteins on a case-by-case basis. With antibody intracellular delivery hurdles yet to be overcome, most current therapeutics and diagnostic whole antibody targets are extracellular antigens. There are many factors within extracellular targets such as posttranslational modification which can underlie the suitability and recognition of the epitopes. In one example, glycosylation has been shown to impede antibody recognition in both the ongoing COVID-19 pandemic [31] and HIV [32] when the modification occurred at epitope sites. Thus, even with good epitope bioinformatics-calculated prediction scores or good experimental results from bacterial produced nonglycosylated proteins (with rare exceptions), care must be taken for the effects of posttranslational modification on occluding or interfering with antibody recognition. Epitope occlusion, in the form of cryptic epitope or cryptotopes [33] , has been reported in numerous viruses such as the Norovirus [34] , Influenza [35] , Ebola [36] , and HIV (Fig. 1A) , where apart from posttranslational modifications methods, HIV utilized the hypervariable regions in the Env to occlude gp41 epitopes [37] from immune detection. On self-antigens, such occlusion of inducible cryptic epitopes plays a role in reducing autoimmunity [38] , but in some cases can be exploited for differentiating disease states when the cryptotopes are exposed during pathogenesis e.g. prion disease [39] . In the unlikely situation where targeting the viral receptor is not easily achieved, blocking the viral target (host cell receptor) from viral spike binding can be performed, as in the case for SARS-CoV-2 [40, 41] and poliovirus [42] . However, care is needed to avoid affecting host cell activity by the antibody through over activation (e.g. by the mimicking of receptor stimulation and inducing of altered signaling, as well as the triggering of uncontrolled microthrombosis, cell lysis and neutrophil activation [43] ) or preventing activation (e.g. inhibiting hyaluronan clearance by liver cells [44] ). Some antigens have the potential to bind antibodies at nonconventional complementarity determining regions by inducing binding pockets or the formation of stretches/patches on antibodies. This phenomenon was observed for nonconventional immunogenic molecules such as nickel [45] binding to trastuzumab and pertuzumab that can possibly underlie the disease pathogenesis of nickel type-I allergy. The other example of induced binding is in the molecular dynamics simulation of Trastuzumab binding to Her2, inducing a cryptotope that facilitated pertuzumab binding [46] . Although the synergism between the two clinical therapeutics (pertuzumab and trastuzumab) was shown experimentally to be due to the different epitopes on the The location of the epitope on the target antigen can affect its accessibility by antibodies that are conjugated or multimeric in nature due to steric hindrances. This is evident in internal epitopes that would not be accessible to multimeric IgMs but accessible to monomeric Igs or antibody fragments. (C) Steric hindrances for multivalent binding by antibodies due to the location of the epitope on the target antigen. Created with BioRender.com. same antigen without evidence of an induced pertuzumab epitope [47] , the possibility of occluded epitopes ought to be sagaciously considered depending on the desired application and utility. Apart from access at the intra/extracellular level and occluded/induced epitopes, obvious steric hindrances at the molecular level to access epitopes can impact the efficacy of the antibody (Fig. 1B) . Although the earlier mentioned example [46, 47] showed synergistic binding of trastuzumab and pertuzumab simultaneously to their different epitopes on Her2, steric hindrances resulting from multiple whole or conjugated or multimeric antibodies are known to arise in flow cytometry [48] . When made into multimeric IgMs for multiple antigen binding, trastuzumab IgM could not have full occupancy of its Fab regions due to steric hindrances binding to multiple Her2. This was, however, not in the case for pertuzumab IgM [49] , which showed higher avidity effects [50] . The sheer size of multimeric antibodies can be advantageous in agglutination for immune clearance and result in a better therapeutic or diagnostic than the traditional IgG, as evidenced in a nasal delivery for SARS-CoV-2 [51] . Yet, this advantage requires further studies for possible steric hindrance (Fig. 1C) . With IgM being typically used in hemagglutination assays [52] [53] [54] [55] , one need to consider these steric effects on the accuracy of such IgM-based assays. For this reason, the checklist for selecting any epitope for diagnostic or therapeutic application needs to account for molecular accessibility, especially when utilizing larger antibodies (whether conjugated, multimeric, or whole). It should be noted that the textbook primary antibody response is typically IgM and steric hindrances may underlie why IgMs tend to have lower affinities for the antigen. Such steric hindrances also apply to the development of bi-specific antibodies requiring additional optimization [56] such as protein flexibility in accommodating antibodies as reflected recently in the Her2-Her3 extracellular dimer dynamics in the Her2-Her3-Nrg1β complex, which modulated receptor activity and accommodated trastuzumab and pertuzumab binding in vitro [57] . Nonetheless, the potential limitation of multi-specific antibodies by steric hindrances, especially those intended to engage whole cells [58] (the promises of bispecific T-cell engagers (BiTEs) for oncology [59] ), should include accessibility as a major consideration. It is in this area that perhaps a more flexible antibody hinge at the constant heavy 1 domain of antibodies may alleviate some structural constraints as evidenced in immunoglobulin A [60] and other isotypes [50] . Since escape mutations in the epitope result in antibody recognition failure, one key criterion of epitope selection is its conservation and this occurs in at least two levels within the antigen: (1) in the presence of mutations and (2) conservation within the family to allow broad-spectrum targeting (Fig. 2) . The earlier level of mutations within the species or viral type being important is exemplified in the recent COVID-19 pandemic, where SARS-CoV2 spike mutations led to decreased effectiveness of the early vaccines to novel variants [5] . On the intent for broad-spectrum protection, other viral vaccines such as that against the human papillomavirus found to induce cross-neutralizing antibodies [61] to its close relations are potential exploitation areas. Likely due to faster escape mutations in microbial pathogens, there are more monoclonal antibody therapeutics used clinically against cancer than infectious diseases [62] , where those targeting the latter tend to be polyclonal [63] , perhaps in an attempt to cover more epitopes to mitigate escape. Nonetheless, in the search for conserved regions in pathogens, large sequence databases can provide some insights (such as HBVdb [64] , Los Alamos Hepatitis C Virus [65] , hemorrhagic fever virus [66] , HIV [67, [68] [69] , Global Initiative on Sharing All Influenza Data [70] , and Nextstrain [71] ), but bearing in mind the constraints of extracellular targets for both neutralization of viruses for therapeutics and detection in diagnostics that do not require preprocessing to release internal intracellular contents. It should be noted that broad-spectrum targets of such nature are expectedly limited due to viral tropism. With some foresight, overcoming cellular level accessibility issues e.g. intrabodies or novel delivery methods to target intracellular targets, conserved viral epitopes can expand to viral enzymes [72] for better search of potential broadspectrum antivirals. This can be performed by searching for substrate analogues sites that indicate the presence of a functional domain [73, 74] that would be more conserved due to the preservation of enzyme functions. In antibodies, the conserved target of omalizumab (Xolair ® ) to immunoglobulin E (IgE) fragment crystallizable (Fc) enabled it to be effective against type I allergies of varying allergen-specific IgEs [75] . Since the constant (C) region of antibodies is generally more conserved, omalizumab was sagaciously raised against the C-region, overlapping with the FcεRIα binding site. Although the existence of allotypes in the Cε-regions [76] can be an issue, other possible effects recently reported include the allosteric communication from the variable (V) region that could influence FcεRIα interaction [77] . Despite overlapping with the binding site of FcεRI, the V-region distal allosteric effects had negligible effects on omalizumab binding. This makes the omalizumab binding site a sagacious epitope that displayed the following benefits: (1) at a conserved region across various pathogenic factors (different allergen-specific IgEs); (2) at the allosterically insulated regions that are unaffected by the changes at hypervariable regions; and (3) relatively immutable. The need for conserved epitopes in the face of mutations is evidenced by the challenge to existing COVID-19 vaccines by new variants [78] . In the lack of conserved epitopes, the alternative option is to preemptively predict possible escape mutations in the epitopes and design interventions that would remain effective against them. This is not only easily performed through recombinant or peptide methods discussed above, but even easier to implement as vaccines with the clinical use of mRNA vaccines [79] . Despite the lack of their reported incorporation for therapeutic or diagnostic development, the use of prediction methods in vaccines has been in place for a while with the example of the successful poliovirus RdRp in vitro platform [80] and similar attempts for HFMD causative enterovirus (EV) A71 [81] . These methods of predicting new mutations exhibit great promise despite being in vitro and can benefit from in silico augmentation involving network analysis [82] , robust statistical model building [83] , time series [84] , stacking models [85] , and random signature analysis [86, 87] . Nonetheless, it should be noted that EVs and lentiviruses like HIV mutate through recombination, whereas influenza utilizes the method of assortment (in the Orthomyxovirus family), requiring further research to get to a unified computational method capable of dealing with RNA viruses of different mutation methodologies. For reassortment viruses, it is important to consider that in silico simulations of the reassortment would be more efficient and safer to generate given the need of Antibody Therapeutics, 2022 67 Figure 2 . Conservation of epitopes across highly mutable target antigens (top) would reduce the chance of escape mutations even as the pathogen accumulates mutations in the target antigen to prolong the effectiveness of the antibody in either detecting or neutralizing the target. Similarly, targeting conserved epitopes that are conserved in similar proteins (e.g. RT) across viruses or pathogens can allow broad-spectrum detection or therapeutic intervention (bottom), improving its market use, while also useful against emerging viruses utilizing replication enzymes e.g. RT or RdRp of the same protein family. Created with BioRender.com. coinfection which can be constrained experimentally by biosafety and bioethics concerns in possible gain-offunction experiments. Regardless of the methodology approach, key features of tropism changes or polymerase activity in respiratory viruses, such as that mentioned for H5N8 [88] would be areas of importance to focus in species jumping and pandemic potential. Experimentally, there is already notable progress in predicting mutations through the understanding of molecular biology and the innate biases of polymerases involved in replication (Fig. 3) . Although such predictions are often deemed stochastic and unpredictable [89] , recent evidence has shown that certain propensities in the genetic code [90] can confer a degree of predictability. It is with more knowledge about the target biology that renders mutations in the given target less stochastic and more predictable. This is demonstrated recently where even without immune or drug selection pressures, HIV RT displayed clear mutational biases in specific locations in HIV genes within a mimic of a single replication cycle [91] . Given that a high percentage of the generated mutations were found in patients, the rise of drug-resistant mutations in the HIV genes was not entirely stochastic nor required intense immune or drug selection pressures to emerge. As the 'Godzilla' of fast mutating viruses, HIV studies showed that the crossresistance for protease inhibitors [92] and RT inhibitors [93] could be investigated by network analysis. Such analysis has showed how mutations can confer cross-resistance beyond one inhibitor. Thereby, in the vein of MID3, structural modeling studies can reveal the possible effects of emerging mutations with respect to drug resistance given the constraints of the mutations to the functional fitness of the target. It is also in this area that therapeutics which augments lethal mutagenesis for error catastrophe [94, 95] could be an alternative strategy for undruggable targets. Although alternative strategies exist in noncompetitive non-nucleoside RT inhibitors or non-nucleoside reverse transcriptase inhibitors (NNRTIs) [96] and the abovementioned lethal-mutagenesis-based [97] , the direct inhibition of targets is the most common therapeutic approach. This approach puts the focus of epitopes at the active/functional sites as key targets, to which the consideration of the antibody therapeutics over small molecules can augment the success given that larger molecules may inhibit binding better. From the example of steric hindrances of trastuzumab and pertuzumab IgM to Her2, pertuzumab IgM inhibited cell proliferation better than pertuzumab IgG1 [49] . This was interesting because pertuzumab IgM being larger/multimeric, may have prevented Her2 homodimerization/activation (necessary for its oncogenic effects [98] ) better than its IgG1 counterpart based on the stoichiometry of binding sites. This is especially so given that the experiments were performed in vitro without effector immune cell effects and that unlike the trastuzumab binding site [99] , the pertuzumab binding site directly inhibited homodimerization [100] . [91] ) to determine innate viral polymerase mutation rates, hotspots, and biases for the incorporation into computational methods for better prediction. Such a platform would not only provide insights to the viral polymerases for augmentation toward lethal mutagenesis, but also improve preparedness to identify conserved regions and identify potential mutations of concern that can lead to escape to guide drug and diagnostic kit development. Created with BioRender.com. Through steric hindrances by larger IgM molecules, the selection of epitopes directly at/near the active or dimerization sites allows for direct therapeutic inhibition as opposed to mere immune tagging for immune effector cells. When coupled with the use of multimeric or conjugated antibodies, better direct effects can be achieved (Fig. 4A) . Such an approach is relevant not only in oncology where over expressed biomarkers lead to proliferation, but also in infectious diseases to neutralize virus attachment and entry [101] . With further understanding of viral spike proteins, epitopes that facilitate direct inhibition of potentially deadly viral pathological effects ought to be considered. Given the recent evidence showing the SARS-CoV-2 spike to also possess superantigenic characteristics that can cause hyperinflammation leading to multisystem inflammatory syndrome in children and cytokine storms in adult COVID-19 [102] , such regions can be targeted through epitope selection of the region for mitigating the clinical morbidity, providing a much needed paradigm shift in treating viral infections [103] with pathogenesis roots in superantigenic activity. Although antibodies may already bind to such superantigenic motifs sites, further in-depth consideration of such recognition sites [104] will facilitate epitope selection for direct viral pathogenicity blocking in addition to that of attachment and entry. Evident from oncology and HIV virology requiring multi-pronged interventions, where in the former, there is combinatorial synergistic use of trastuzumab and pertuzumab [47, 105] , and in the latter, combinatorial antiretroviral therapy [106] , there is still much to consider in additional epitopes. The addition of more targetable sites within the same antigen especially in the absence of other ideal epitopes for direct intervention can open the way for allosteric epitopes. With clear lessons in the use of NNRTI and recent evidence of a non-enzymatic subunit being potentially druggable in HIV [107] , distal epitopes can be used to influence the enzyme/target's active sites by noncompetitive antibodies (Fig. 4B) . Allosteric sites may not necessarily elicit major shape changes [108] and are thus often modulators than direct activators/inhibitors (see examples: IgE [77] , FcεRI [109] , IgA [60] , microbial targets [110] ). Such dampening of activity in oncology or virology can be what is precisely required at times to permit the necessary gain by the immune system. Furthermore, these allosteric sites in microbial and viral proteins could drive function-crippling mutations through drug resistance mechanisms at the drug binding site such as the non-cleavage site mutations contributing to drug resistance-associated protein fitness compensation in HIV-1 Gag [111, 112] . These sites could also be epitopes for intrabodies to prevent drug resistance or reduce protein fitness and be leveraged upon to dissociate already bound complexes by increasing dissociation (e.g. in the case of IgE-FcERI [109] ). With additional areas to target apart from active/functional sites, it may be easier to find common allosteric sites to develop broad-spectrum targeting antibodies. Such widening of the search field may overcome constraints present in extracellular localization. Although the allosteric analysis is currently most effectively performed computationally, it is important to be as inclusive of the entire protein structure/model as holistically possible to study the distal effects in a counterintuitive manner when compared with raising antibodies based on the reductionist approach in experiments of using purified recombinant proteins, regions, or peptides. Although new databases such as the Post Translational Modification Structural Database [113, 114] now including annotated posttranslational Choosing the epitopes for their potential functionality to (A) directly inhibit dimerization in the case of Pertuzumab IgM inhibiting Her2 dimerization better than its IgG counterpart due to its larger size [49] . In such cases, an antibody fragment such as a Fab alone may not necessarily provide sufficient steric hindrance to block dimerization. In the event where direct blocking may not be suitable, the targeting of (B) allosteric epitopes to influence active or functional sites can also be explored to increase target areas in the same target antigen. Created with BioRender.com. modification, the majority of crystal/nuclear magnetic resonance structures in structural databases without posttranslational modifications can result in incongruency of findings, especially when using cell lines with varying glycosylation patterns [115] . Sagacity in target and epitope can maximize the success of vaccine, therapeutics, and diagnostic development. Apart from deep understanding of the target biology, there are numerous factors ranging from accessibility at the cellular and molecular level, the inducibility, occlusion, conservation at family and species levels, influence by distal allosteric sites. Given the diversity of these factors, there will inevitably be difficulties for direct comparisons and implementations, especially when they should be considered holistically. Coupling such efforts with sagacious antibody development [6] involving swapping of elements [116] , expensive developmental processes can be made more cost and time efficient. 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The authors would like to thank Zealyn Shi-Lin Heng for proofreading the manuscript.