key: cord-0970486-umamkud9
authors: Cheranev, Valery; Loginova, Maria; Jankevic, Tatjana; Kutyavina, Svetlana; Rebrikov, Denis
title: A novel allele, HLA‐C*15:227, identified when typing COVID‐19 patients
date: 2021-02-08
journal: HLA
DOI: 10.1111/tan.14199
sha: 05ba96131d3b0a6d82db1dc22f47b2a09bde16fd
doc_id: 970486
cord_uid: umamkud9

HLA‐C*15:227 differs from HLA‐C*15:02:01:01 by a single nonsynonymous change (368A → G Tyrosine 99 to Cysteine).

HLA-C*15:227 differs from HLA-C*15:02:01:01 by a single nonsynonymous change (368A ! G Tyrosine 99 to Cysteine).

HLA-C*15:227, NGS HLA typing, novel HLA allele The IPD-IMGT/HLA Database currently has information on more than 20,000 human leukocyte antigen (HLA) class I alleles and more than 6200 of these are alleles of HLA-C gene. 1 In this report, we describe the sequence of a novel HLA-C allele. It was identified in a COVID-19 patient, using a next-generation sequencing (NGS) method for HLA typing.

The genomic DNA was extracted from peripheral blood cells using a commercial kit "Proba MCh-Max," according to the manufacturer's instructions (DNA-technology, Moscow, Russia) on automatic DNA extraction station "DT-stream" (DNA-technology, Moscow, Russia). It was typed for HLA-alleles at the A, B, C, DRB1, and DQB1 loci using reagents "HLA-expert" (DNA- The novel allele was submitted to GenBank (accession number MT896389) and the IPD-IMGT/HLA Database 1 and was officially assigned by the World Health Organization (WHO) Nomenclature Committee for Factors of the HLA System in September 2020. List of such new names were published in the following WHO Nomenclature Report. 2 The HLA-C*15:227 allele was confirmed in the Research Laboratory of Applied Immunogenetics by NGS typing with the VariFind HLA solution IL kit (Parseq Lab Co, Saint Petersburg, Russia), the whole HLA-C (except the 3 0 -UTR region) gene was amplified by long PCR at 1st step. Once the library for NGS was obtained, it was then sequenced using a MiSeq (Illumina, The United States) and analyzed by VariFind HLA Software v2.2 (Parseq Lab Co, Saint Petersburg, Russia).

IPD-IMGT/HLA database

Nomenclature for factors of the HLA system

A novel allele, HLA-C*15:227, identified when typing COVID-19 patients

ACKNOWLEDGMENT This research was funded by grant number 075-15-2019-1789 from the Ministry of Science and Higher Education of the Russian Federation allocated to the Center for Precision Genome Editing and Genetic Technologies for Biomedicine.

The authors declare no conflicts of interest.