key: cord-0969166-apowyq7i
authors: Stahl, Klaus; Bräsen, Jan Hinrich; Hoeper, Marius M.; David, Sascha
title: Absence of SARS-CoV-2 RNA in COVID-19-associated intestinal endothelialitis
date: 2021-01-03
journal: Intensive Care Med
DOI: 10.1007/s00134-020-06326-6
sha: d6787681ab4a75775b6a57d9aec4a9b6ad66c8cd
doc_id: 969166
cord_uid: apowyq7i

nan

Fluorescent in situ hybridization using RNA-SARS-CoV-2 HuluFISH PLUS probe tagged with Atto565 (MetaSystems Probes, Altlussheim, Germany). Abundant positive signals can be seen in lung specimen of a patient who died from Covid-19 pneumonia (red signals, a). In the gut specimens of our patient, vessels including those with severe endothelialitis were completely negative (b); only single signals in cells located within interstitium can be seen (c) (micrographs by courtesy of Jessica Schmitz, Nephropathology Unit, Institute of Pathology, MHH) demonstration of massive intestinal endothelialitis and microvascular thrombosis is of high clinical relevance for intensivists fighting COVID-19. Evidently, some patients with severe COVID-19 develop a systemic vasculitis, the pathogenesis of which is not yet understood but might be triggered by indirect effects rather than direct viral infection.

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Electron microscopy of SARS-CoV-2: a challenging task

Direct evidence of SARS-CoV-2 in gut endothelium

Hunting coronavirus by transmission electron microscopy -a guide to SARS-CoV-2-associated ultrastructural pathology in COVID-19 tissues