key: cord-0966413-0oxy9b1p authors: Hastie, Claire E.; Mackay, Daniel F.; Ho, Frederick; Celis-Morales, Carlos A.; Katikireddi, Srinivasa Vittal; Niedzwiedz, Claire L.; Jani, Bhautesh D.; Welsh, Paul; Mair, Frances S.; Gray, Stuart R.; O'Donnell, Catherine A.; Gill, Jason MR.; Sattar, Naveed; Pell, Jill P. title: Vitamin D concentrations and COVID-19 infection in UK Biobank date: 2020-05-07 journal: Diabetes Metab Syndr DOI: 10.1016/j.dsx.2020.04.050 sha: d92b23fd3daf619151bb02e0d6874ed6542cfa75 doc_id: 966413 cord_uid: 0oxy9b1p Abstract Background and aims COVID-19 and low levels of vitamin D appear to disproportionately affect black and minority ethnic individuals. We aimed to establish whether blood 25-hydroxyvitamin D (25(OH)D) concentration was associated with COVID-19 risk, and whether it explained the higher incidence of COVID-19 in black and South Asian people. Methods UK Biobank recruited 502,624 participants aged 37–73 years between 2006 and 2010. Baseline exposure data, including 25(OH)D concentration and ethnicity, were linked to COVID-19 test results. Univariable and multivariable logistic regression analyses were performed for the association between 25(OH)D and confirmed COVID-19, and the association between ethnicity and both 25(OH)D and COVID-19. Results Complete data were available for 348,598 UK Biobank participants. Of these, 449 had confirmed COVID-19 infection. Vitamin D was associated with COVID-19 infection univariably (OR = 0.99; 95% CI 0.99–0.999; p = 0.013), but not after adjustment for confounders (OR = 1.00; 95% CI = 0.998–1.01; p = 0.208). Ethnicity was associated with COVID-19 infection univariably (blacks versus whites OR = 5.32, 95% CI = 3.68–7.70, p-value<0.001; South Asians versus whites OR = 2.65, 95% CI = 1.65–4.25, p-value<0.001). Adjustment for 25(OH)D concentration made little difference to the magnitude of the association. Conclusions Our findings do not support a potential link between vitamin D concentrations and risk of COVID-19 infection, nor that vitamin D concentration may explain ethnic differences in COVID-19 infection. • A novel coronavirus that manifested in 2019 (SARS-CoV-2) has led to a pandemic of pneumoniarelated illness . There is an urgent need to better understand risk factors for contracting the infection and for poorer prognosis thereafter. • Evidence is emerging that COVID-19 disproportionately affects black and minority ethnic individuals, but the underlying mechanism is unknown. One potential mediator could be their higher prevalence of apparent vitamin D deficiency. • We explored whether blood 25 hydroxyvitamin D (25(OH)D) concentration was associated with COVID-19 risk among UK Biobank participants, and whether this explained wholly, or in part, the higher incidence of COVID-19 infection in ethnic minority participants. • Our analyses of UK Biobank data provided no evidence to support a potential role for (25(OH)D) concentration to explain susceptibility to COVID-19 infection either overall or in explaining differences between ethnic groups. COVID-19 and low levels of vitamin D appear to disproportionately affect black and minority ethnic individuals. We aimed to establish whether blood 25-hydroxyvitamin D (25(OH)D) concentration was associated with COVID-19 risk, and whether it explained the higher incidence of COVID-19 in black and South Asian people. States a pattern of higher risk has been observed in African Americans. 3 Consequently, the relationship between ethnicity and COVID-19 has been identified as an urgent public health research priority. 4 Several factors have been proposed to explain the apparent greater risk of COVID-19 infection in ethnic minority groups. UK Government statistics show that people from black and minority ethnic backgrounds are more likely than white British people to live in the most socioeconomically deprived areas of England. 5 Furthermore, certain minority ethnic groups experience a higher burden of comorbid disease, 6 which may put them at higher risk of more severe COVID-19 infection. 7, 8 One potential mediator could be the higher prevalence of apparent vitamin D deficiency in black and minority ethnic populations. 4 Vitamin D is variably inversely associated with multiple health outcomes and mortality (although these associations may not be causal). 9 Most vitamin D results from production in the skin following exposure to ultraviolet (UV) radiation from the sun. Individuals with dark skin have, on average, lower concentrations of blood vitamin D because the melanin in dark skin does not absorb as much UV. 10 Furthermore, deficiency is more common in high latitude countries such as the UK. Whilst most chronic conditions have not been improved by vitamin D supplementation, a recent meta-analysis of randomised trials suggested vitamin D may lessen the risk of acute respiratory infections. 11 In this study, we hypothesised that blood 25 hydroxyvitamin D (25(OH)D) concentration was associated with COVID-19 risk among UK Biobank participants, and explained wholly, or in part, the higher incidence of COVID-19 infection in ethnic minority participants. UK Biobank recruited 502,624 participants aged 37-73 years across England, Scotland and Wales between 2006 and 2010. Its aim was to identify the causes of disease and death in middle and old age by following up participants over time. At baseline, biological measurements were recorded and touch-screen questionnaires administered according to a standardised protocol. 12, 13 The study received ethical approval from the North West Multi-Centre Research Ethics Committee (REC reference: 16/NW/0274), and was conducted in accord with the principles of the Declaration of Helsinki. All participants gave written informed consent for data collection, analysis, and record linkage. Baseline data from UK Biobank were linked to COVID-19 test results provided by Public Health England, 14 including the specimen date, origin (whether the person was an inpatient or not) and result (positive or negative). Confirmed COVID-19 infection was defined as at least one positive test result. Data were available for the period 16th March 2020 to 14th April 2020. Exposures were measured at the baseline assessment visits conducted between 2006 and 2010. Blood collection sampling procedures for the study have previously been described and validated. 15 Biochemical assays, including 25(OH)D, a measure of vitamin D status, were performed at a central laboratory on around 480,000 samples. Further details of these measurements can be found in the UK Biobank Data Showcase and Protocol. 16 Vitamin D was imputed with the minimum detectable value (10nmol/L) if it was below the limit of detection, and the maximum detectable value (375nmol/L) if too high for detection. Ethnicity was self-reported, and categorised as white, black, South Asian, or other. Smoking status was self-reported and categorised as current or non (ex/former) smoker. Blood pressure was measured at the baseline visit using an automated measurement, and the average of available measures used. Height was measured using a Seca 202 height measure. Weight was measured to the nearest 0.1 kg using the Tanita BC-418 MA body composition analyser. Body mass index (BMI) was derived from weight(kg)/height(m) 2 . It was categorised into underweight (BMI<18.5kg/m 2 ), normal weight (BMI 18.5-24.9 kg/m 2 ), overweight (BMI≥25-29.9 kg/m 2 ) and obese (BMI≥30 kg/m 2 ). Area-level socioeconomic deprivation was assessed by the Townsend score (incorporating measures of unemployment, non-car ownership, non-home ownership and household overcrowding). 17 Higher scores on the Townsend score represent greater socioeconomic deprivation; scores were categorised into quintiles. Diabetes at baseline was defined as self-reported physician-diagnosed type 1 or type 2 diabetes, a primary care or hospital record of diabetes at or before recruitment (defined as ICD-10 codes E10-E14.9), or diabetes medication. Household income was self-reported and categorised into: <£18,000; £18,000-£30,999; £31,000-£51,999; £52,000-£100,000; or >£100,000. Health was self-rated as excellent, good, fair, or poor. Long-standing illness, disability or infirmity was self-reported as yes or no. Univariable logistic regression analysis was performed of the association between 25(OH)D concentration (as a continuous variable) and confirmed COVID-19 infection. The model was then adjusted for sex, month of assessment, Townsend deprivation quintile, household income, self-reported health rating, smoking status, BMI quintile, ethnicity, age at assessment, diabetes, systolic blood pressure (SBP), diastolic blood pressure (DBP), and long-standing illness, disability or infirmity. As sensitivity analyses these models were repeated with participants categorised as vitamin D deficient (defined as <25nmol/L) or not deficient 18 and then categorised as vitamin D insufficient (defined as <50nmol/L) or sufficient. Next, univariable logistic regression was performed of the association between ethnicity and confirmed COVID-19 infection. The model was first adjusted for 25(OH)D, then sex, month of assessment, Townsend deprivation quintile, household income, self-reported health rating, smoking status, BMI quintile, age at assessment, diabetes, SBP, DBP, and long-standing illness, disability or infirmity. Finally, multivariable analysis was performed including an ethnicity*vitamin D deficiency interaction term. All analyses were undertaken using Stata v14. (Table 3) . Results were similar when, instead of adjusting for 25(OH)D concentration, adjustment was made separately for vitamin D deficiency and vitamin D insufficiency. There was no significant interaction between ethnicity and vitamin D deficiency (OR=0.90; 95% CI=0.66-1.23; p-value=0.515). Our findings are consistent with previous studies 7, 19 It has been suggested in some media outlets that language barriers may contribute to ethnic differences in COVID-19 risk. This is unlikely to contribute to the risk we observed in UK Biobank because all participants spoke English (albeit with the possibility of fluency variation). The association with ethnicity was only slightly attenuated after adjustment for socioeconomic and lifestyle differences in white and black and minority ethnic participants; the risk of COVID-19 remained around 4-fold in black participants and more than 2-fold in South Asians. Further studies are required to determine the mechanisms underlying ethnic variations in risk of COVID-19 infection and its severity. It may be that pathways related to cardiometabolic conditions or differences in cardiorespiratory reserve, or potentially other social factors, are more relevant, as we have recently discussed. 23 Our study replicated findings showing increased risk of COVID-19 in black and minority ethnic individuals, in men, and in people who are overweight or obese. It is surprising that we did not observe an association between diabetes or blood pressure and COVID-19 risk. Other studies have shown increased risk of hospitalisation and severe illness requiring ventilation in patients with diabetes and hypertension. 7, 24 However, ours is a relatively healthy general population cohort. Furthermore, we do not have information on the severity of COVID-19 and have included all positive tests rather than only severe cases. We did not show an independent association between smoking and COVID-19. Evidence from the literature is mixed. Initial studies suggested that current smoking increases the risk of severe infection. 25 However, this has since been disputed with some evidence that smoking may even protect against initial infection. 26 The strengths of UK Biobank include its extensive phenotyping which enables the adjustment for demographic and lifestyle risk factors, disease and ill health, its large sample size, and its central processing laboratory for biochemical assays. However, it is not representative of the general population, in that participants live in less socioeconomically deprived areas, are predominantly Caucasian, and have fewer self-reported health conditions. 27 year, and generally track over time. 28 Our study is the first to assess whether there is an association between blood 25(OH)D concentration and COVID-19 risk. We found no such link, suggesting that measurement of 25(OH)D would not be useful to assess risk in clinical practice. Furthermore, our results suggest that vitamin D is unlikely to be the underlying mechanism for the higher risk observed in black and minority ethnic individuals and vitamin D supplements are unlikely to provide an effective intervention. Our analyses of UK Biobank data provided no evidence to support a potential role for 0.029 OR odds ratio; CI confidence interval *also adjusted for sex, month of assessment, Townsend deprivation quintile, household income, self-reported health rating, smoking status, BMI category, age at assessment, diabetes, SBP, DBP, and long-standing illness, disability or infirmity Covid-19 study case mix programme; 2020. 2. Office for National Statistics John Hopkins Coronavirus Resource Centre Is ethnicity linked to incidence or outcomes of covid-19? Ethnicity facts and figures CVD risk factors and ethnicity--a homogeneous relationship? 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Qeios Comparison of Sociodemographic and Health-Related Characteristics of UK Biobank Participants With Those of the General Population Intraindividual variation in plasma 25-hydroxyvitamin D measures 5 years apart among postmenopausal women ) body mass index; IQR inter-quartile range DBP diastolic blood pressure Manuscript title: Vitamin D concentrations and COVID-19 infection in UK Biobank Authors United Kingdom 2 Institute of Cardiovascular and Medical Sciences JPP and NS had the original concept. CEH undertook the statistical analyses. All authors interpreted the results. CEH, JPP and NS drafted the manuscript. All authors revised the manuscript and approved the final version. The authors declare no conflict of interest.