key: cord-0965853-kh34pv2w
authors: Rabbani, Masud; Haque, Munirul M.; Das Dipal, Dipranjan; Zarif, Md Ishrak Islam; Iqbal, Anik; Schwichtenberg, Amy; Bansal, Naveen; Soron, Tanjir Rashid; Ahmed, Syed Ishtiaque; Ahamed, Sheikh Iqbal
title: An mCARE study on patterns of risk and resilience for children with ASD in Bangladesh
date: 2021-11-01
journal: Sci Rep
DOI: 10.1038/s41598-021-00793-7
sha: ea78a7efc3ee8af1c540134a2e26cb66349cbee8
doc_id: 965853
cord_uid: kh34pv2w

Community-wide lockdowns in response to COVID-19 influenced many families, but the developmental cascade for children with autism spectrum disorder (ASD) may be especially detrimental. Our objective was to evaluate behavioral patterns of risk and resilience for children with ASD across parent-report assessments before (from November 2019 to February 2020), during (March 2020 to May 2020), and after (June 2020 to November 2020) an extended COVID-19 lockdown. In 2020, our study Mobile-based care for children with ASD using remote experience sampling method (mCARE) was inactive data collection before COVID-19 emerged as a health crisis in Bangladesh. Here we deployed “Cohort Studies”, where we had in total 300 children with ASD (150 test group and 150 control group) to collect behavioral data. Our data collection continued through an extended COVID-19 lockdown and captured parent reports of 30 different behavioral parameters (e.g., self-injurious behaviors, aggression, sleep problems, daily living skills, and communication) across 150 children with ASD (test group). Based on the children’s condition, 4–6 behavioral parameters were assessed through the study. A total of 56,290 behavioral data points was collected (an average of 152.19 per week) from parent cell phones using the mCARE platform. Children and their families were exposed to an extended COVID-19 lockdown. The main outcomes used for this study were generated from parent reports child behaviors within the mCARE platform. Behaviors included of child social skills, communication use, problematic behaviors, sensory sensitivities, daily living, and play. COVID-19 lockdowns for children with autism and their families are not universally negative but supports in the areas of “Problematic Behavior” could serve to mitigate future risk.

www.nature.com/scientificreports/ In Bangladesh, the COVID-19 impacts on children's mental health, especially by depression, anxiety, and sleeping disorder. Approximately 19.3% of Bangladeshi children were disturbed moderately, and about 7.2% were severely suffered by COVID-19 29 . This children's mental disturbance is also linked to parents' stress and abnormal behavior, especially for the children with ASD. Children with ASD were at risk for delays during the lockdown period 8 . According to the DSM-5 30 , new lifestyle changes can create difficulties in their behavioral development. Several studies 1, 8, 10, 18, [31] [32] [33] document that the COVID-19 confinement impacts the psychological wellbeing of children with ASD. Most studies were based on surveys or focus-group interviews from parents. But behavioral changes in a child with ASD can be a long process, so to get a better picture of long-term impacts, prospective longitudinal studies are needed on children with ASD. Our aim in this study was to document the impact of the COVID-19 lockdown on specific behavioral parameters of children with ASD.

Our work is the first prospective longitudinal study to examine the COVID-19 lockdown effect on the specific behavioral parameter in ASD within a Low-Middle-Income-Countries like Bangladesh. This study is based on the mCARE (Mobile-based care for children with ASD) project 34, 35 , which monitored 150 children with autism, capturing behavioral data for one year (from November 2019 to November 2020). In Bangladesh, the first lockdown took place from March 2020 to May 2020 36 . The timeframe of our study provided us with pre-lockdown (from November 2019 to February 2020), lockdown (March 2020 to May 2020), and post-lock down (June 2020 to November 2020) participant behavioral data. Using the mCARE tools over the course of the pandemic restrictions, health care professionals could take proper care and employ evidence-based decision-making remotely 34 . This study aims to evaluate parent-report behavioral patterns of risk and resilience for a group of children with ASD before, during, and after an extended COVID-19 lockdown. The main aim of mCARE was to monitor the behavioral and milestone parameter of the children with ASD by remote sampling method using the mobile app. The project was held in Bangladesh for a year, and in this time period, the COVID-19 pandemic was broken out and, several countries were locked down including, Bangladesh. In this study, we used the mCARE test group children's behavioral data to find the research question that "How lockdown due to COVID-19 impacts on children with ASD?". We hypothesis that "The lockdown due to COVID-19 has more negative behavioral impact than positive impact on children with ASD". However, it has some positive impacts on children with ASD.

In this study, we evaluate the behavioral changes (patterns of risk and resilience) for 150 children with ASD in Bangladesh. We used the mCARE 34, 35 data and divided the data into three time-based segments (pre-lockdown, lockdown, and post-lockdown data) based on the COVID-19 lockdown period in Bangladesh. After that, we analyzed the data and compared the behavioral patterns of risk and resilience. mCARE. In this project, a total of 300 children with ASD were recruited from four major centers in Bangladesh. We have selected these four centers, there are the major Autism Development centers in Bangladesh, and we recruited these children by proper consent from these centers, and we got 95% of positive responses from the parents of children with ASD. Besides, we also took the IRB from Marquette University Institutional Review Board on July 9, 2020, with the protocol number HR-1803022959 for recruiting these children. Then we have divided two groups: (a) test group: 150 children with ASD are in this group who are regularly monitored by the mCARE system. (b) control group: 150 children with ASD are in this group who are not monitored regularly by the mCARE system, but they were in the system to compare with the test group children. We selected the children for these groups randomly from our cohort. In this 300 cohort, we selected children aged from 2 to 9, and where 79.7% were male, and 20.3% were female, 61.9% were active smartphone users (APP users), and the rest of 38.1% used non-smartphone users (SMS users). We observed that parents were interested in mCARE: APP using, as they feel easier use in mCARE: APP. And we also observed that mCARE: APP users responded higher and more accurately than mCARE: SMS users. We have recruited all demographic children with ASD, such as single parents who have only fathers or mothers. Recruitment had been done by voluntary participation by the legal guardians and will be conducted through the care practitioners. In the recruiting process, we have faced the challenge of training the parents, but by focus group discussion, we overcame the problem. We have analyzed the practitioner satisfaction based on paired t-test 37 . In this study, we have used the "test group" children data to evaluate the behavioral changes in the COVID-19 period.

Research design. In this study, we analyzed the data from the mCARE project, collected from November 2019 to November 2020. mCARE, an innovative longitudinal monitoring digital platform, was funded through a grant from the National Institutes of Health (NIH) 38 and implemented in Bangladesh. The mCARE tools included an mCARE: APP (for smartphone-based service) and mCARE: SMS (a short message service) for families with a cell phone without a 'smart' interface. The mCARE project was run for one year with different activities. Our mCARE project included data from the pre-lockdown, lockdown, and post-lock down periods in Bangladesh 36 . In this study, we segmented these data into three sections (pre-lockdown, lockdown, and postlockdown data) and analyzed the behavioral impact of the children with ASD by these groups of data.

Participants. In this study, we have analyzed behavioral data from 150 ASD children enrolled in the "test group" of the mCARE project. In the mCARE project, we enrolled 300 children with ASD from Bangladesh between 2 and 9 years of age with approval from Marquette University's Institutional Review Board (Protocol number HR-18030222959). We took the informed consent from the parents and/or legal guardians of all subjects (as all subjects were under 18 years) in mCARE to analyze their data for research purposes. Children with ASD were recruited from the four major institutes of Bangladesh in two geographical locations-Dhaka and Chittagong. Typically, families in Bangladesh with low and high socioeconomic resources receive treatment Table 1 shows the patient distribution (only test group) among the four centers. We have incorporated diversity in terms of age, sex, ASD severity, and family socioeconomic resources. From NIMH and IPNA, we recruited 100 caregivers of ASD kids from each. The participants have been divided into two groups-mCARE-APP (50) and mCARE-SMS (50). Each group was further divided equally into test (25) and control (25) groups. Similarly, from Nishpap and AWF, 50 participants have been chosen from each school divided into a test group (25) and a control group (25) . The power analysis yields that the sample size of 300 is sufficient to discover a difference among mCARE-SMS, mCARE-APP, and the control groups with the power of 0.97 at α = 0.05 and the medium effect size. The power analysis was performed using GPower 3.1 based on the ANOVA with all covariates, including demographic variables, care facilities, and care practitioners. (detailed description of the recruitment process in "mCARE" section).

The sociodemographic information of this study is shown in Table 2 . In Table 2 , the total number of participants is 300 (both the test and control groups). These groups were divided into two categories (mCARE: APP www.nature.com/scientificreports/ and mCARE: SMS) based on the mCARE tool application. In this work, we only use the "Test Group" behavioral data to analyze the impact of COVID-19 on children with ASD in Bangladesh.

We deployed the two mCARE tools to collect behavioral parameters based on family phone use/access across the four recruitment locations. We provided the mCARE tools to the primary caregivers (mostly the biological parents of children with ASD).

We assessed 30 behavioral parameters. Initially, the study's care practitioners (clinical coordinators of the four centers) set the behavioral parameters (4-6 parameters for each child) for the children with ASD based on their initial screening and intervention. So about 13.33-20% of behavioral parameters were used to assess every child in this study. After the initial setup, the parents/caregivers began reporting their children's behavioral data based on their child's current behaviors/progress twice a week. A solid data collection plan with proper consent was provided at the begging of the study. All the data was saved in mCARE: DMP (Data Management Platform).

In mCARE, there were 16 clinical coordinators had been recruited for four centers, and for each child with ASD, there was one particular primary caregiver. In this study, the clinical coordinator and caregiver have played a vital role in fulfilling the study's goal. The clinical coordinators are responsible to (i) monitor and utilize the data submitted (in mCARE-DMP) by the caregivers in their treatment process, (ii) follow-up with the primary caregiver if data collection were interrupted, (iii) support the caregivers in any abnormal situation like lockdown in COVID-19, (iv) update the behavioral and milestone parameters of children (with ASD) in the system (mCARE: DMP) based on their condition, (v) communicate with the caregiver, (vi) conduct focus group session with the caregiver, and (vii) report the critical findings to the research team. As the mCARE is a self-reported study for longitudinal monitoring of children with ASD, the primary caregivers (parents) are the key person of the study. Their main role is to (i) report their children's progress based on their condition through mCARE: APP or mCARE: SMS, (ii) take-care their children according to the clinical coordinators' suggestions and ASD knowledge getting from the mCARE, and (iii) contact with the clinical coordinator if there any emergency help needed. The family supports and dwelling environment are two important factors in this study. We observed that those children who belong to fewer siblings and higher income-educated families could get better family care; that reflects on their better improvement level. Similarly, we also observed better improvement in our "test group" children (with ASD) who had better living places, especially in urban rather than rural and congested slums. In the lockdown period due to COVID-19, the children living in congested slums or apartments were more containment than other children who lived in space areas or homes.

Data collection rate. The average data collection rate of mCARE was around 152.19 per week from the four centers. Figure 1 shows the data collection rate throughout the mCARE project timeline. In the data collection phase, we faced several difficulties during the lockdown period.

We observed a low data collection rate (shown in the red box in Fig. 1 ) during the Bangladesh lockdown time. Several reasons were discovered:

I. Several patients returned to their hometowns, mostly in rural areas, where the network connection was unreliable. II. In some cases, for job purposes, the father had to stay separate from the family, taking the single-family mobile phone with him. III. Many parents lost their jobs or had limited income, so they faced difficulties submitting data. We provided all participants monthly incentives throughout the study. www.nature.com/scientificreports/ IV. Family members and caregivers experienced COVID-19-like symptoms and went into isolation in a separate room in the home. This hampered the family from giving regular data feedback. V. Caregivers had mobile phone issues, such as the mobile phone they used to send data was broken or had no balance, and they couldn't repair it or recharge it in time due to the lockdown situation. VI. As the schools were closed due to the lockdown, some of the patients changed schools, and some went to their hometowns permanently.

Our clinical coordinators handled individual problems, talked with participants on their cell phones, and offered assistance and motivation to find solutions for data reporting. As many of the families were in financial crisis in the COVID-19 lockdown period for no job, business, or leaving their home and shifting to the village, our clinical coordinator found these families and helped them financially from the mCARE project fund. It was important to collect proper children's data from the parents to get the impact of COVID-19 on children's mental health. As individual problems were handled and resolved, data collection rates rose among all participants (shown in the green box in Fig. 1 ).

Statistical analysis. The goal of this study was to determine how lockdown impacted children with ASD based on parental behavioral reports. We considered 30 behavioral parameters (both positive and negative based on the behavioral type), and based on the psychiatrist's expertise, we categorized them into four domains (Communication, Social Interaction, Problematic Behavior, and Sensory Sensitivities) (Details in Table 3 ). Parents were asked to provide the rated severity (0 to 10) of each behavior. Parents provided this value using the mCARE: APP or mCARE: SMS). The evaluation window was considered for a year. We divided this window into three different phases (pre-lockdown, lockdown, and post-lockdown) to differentiate the lockdown impact on the children with ASD compared to normal time. Simple descriptive statistics (mean 43 and mode 43 ) were utilized to calculate behavior severity for three different phases. We also calculated the 95% Confidence Interval (CI) for the validation of our results. We used the "Z" value (1.96 for 95% CI) 44 for calculating the 95% CI using the following formula:

where X is the mean; Z is 1.96, chosen from the Z-value table 44 ; S is the standard deviation; n is the sample number in average.

In this study, we have followed our method in accordance with the relevant and standard guidelines and regulations. Table 3 to show the patterns of risk and resilience on the behavioral parameter due to the COVID-19 lockdown. We analysed by both statistical mean (shown in Table 3 ) and mode (shown in Online Appendix Table S1 ) to determine the impact of the COVID-19 lockdown for children with ASD. We had a total of 56,290 behavioral data reports on the sum of all patterns for 150 families over one year (November 2019 to November 2020). We classified these data into three categories: before lockdown or pre-lockdown time (from November 2019 to February 2020), during lockdown (from March 2020 to May 2020), and after-lockdown or post-lockdown (from June 2020 to November 2020). We also categorized the 30 behavioral parameters into two types: positive parameters (7 out of 30) and negative parameters (23 out of 30) (Details in Table 3) .

We calculated the month-wise behavioral severity rate for a particular behavioral parameter by taking the average (using the statistical mean) and most likely value (using the statistical mode). We calculated the prelockdown, lockdown, and post-lockdown for a particular behavioral parameter by averaging the month-wise behavioral severity rate. We calculated the impact table both for the statistical mean (shown in Table 3 ) and mode (shown in Online Appendix Table S1 ) with a 95% Confidence Interval (CI) to validate our result. From Table 3 and Online Appendix Table S1, we found the same impact on every behavioral parameter of the study participants.

In Table 3 , we used the same concept to discover the positive and negative impact on a behavioral parameter due to lockdown. We also categorized our 30 behavioral parameters into four domains: (i) Communication, (ii) Social Interaction, (iii) Problematic Behavior, and (iv) Sensory Sensitivities. For a "Negative" behavioral parameter, if the lockdown period's behavioral rate was higher than the pre-lockdown period's data, then it created a "Negative" impact due to lockdown for that particular behavioral parameter. If the lockdown period's behavioral rate was lower than both the pre-lockdown and post-lockdown period's data, then it created a "Positive" impact. If the behavioral data was consistently decreased, then there was no impact of lockdown for that particular parameter. This rule is the opposite for calculating the impact on the "Positive" behavioral parameters. Table 3 and Appendix Table S1 show the different levels of behavioral data in three different periods. Table 3 has been calculated by Statistical Mean, and Online Appendix Table S1 is calculated by Statistical Mode. Both tables use the same data but in different statistical models. We have used here 30 different behavioral parameters with the average value in a certain time period (Nov-2019 to Feb-2020, March-2020 to May-2020, and June-2020 to Nov-2020). We also classified these 30 behavioral parameters into four specific domains to understand better the COVID-19 impact on children with ASD. Initially, we also categorized the behavioral parameters by positive or negative, based on the behavioral type of the parameter, and it was also used to determine the COVID-19 impact www.nature.com/scientificreports/ on the children with ASD during the lockdown in Bangladesh. Overall from the two tables (Table 3, Online  Appendix Table S1 ), we found the same result of impact rate due to lockdown. We found 13 "Negative" impacts out of 30 behavioral parameters, which is 43.33%, and 5 "Positive" impacts out of 30 behavioral parameters, which is 16.67%. The remaining 12 behavioral parameters had no impact (40%) due to the lockdown. Figure 2 shows the four negatively impacted behavioral parameters on children with ASD during the lockdown period. From Fig. 2a , we can see the aggressive behavior (how often) was increased in the lockdown period; similarly, the lack of concentration (Fig. 2b) , the sensitivity of pain (Fig. 2c) , and sleep problem (Fig. 2d ) also increased on the lockdown time. According to our expert psychiatrists, this increased level of children's behavior has been considered as a negative impact by the COVID-19 lockdown. Figure 3 shows the four positively impacted behavioral parameters on children with ASD during the lockdown period. From this figure, we can see the unusual sensitivity of light (Fig. 3a) , basic needs like hunger (Fig. 3b) , self-injurious behavior (how often) (Fig. 3c) , and inflexible to changes (Fig. 3d) was decreased on the lockdown period, which indicates the positive impact of COVID-19 on the children with ASD. From the two tables (Table 3, Online Appendix Table S1 ), we can see there are 12 behavioral parameters ("No" in the "Impact by Lockdown" column) which has not changed during the lockdown period, and other five behavioral parameters ("Positive" in the "Impact by Lockdown" column) have improved on the lockdown time period in Bangladesh. This refers that COVID-19 lockdown has a resilience impact on the children (with ASD) behavior. On the other hand, the other 13 behavioral parameters ("Negative" in the "Impact by Lockdown" column) have a negative impact, which is surely linked to increasing the parents' stress.

Approval for human experiments. We took the IRB from Marquette University Institutional Review Board on July 9, 2020, with the protocol number HR-1803022959, and titled "MOBILE-BASED CARE FOR CHILDREN WITH AUTISM SPECTRUM DISORDER USING REMOTE EXPERIENCE SAMPLING METHOD (MCARE)" for recruiting a total of 316 subjects, of which we recruited 300. (Details description of participants in the "Methods" section).

Important findings. We found a 43.33% negative impact of lockdown on the behavioral parameters; out of 30 behavioral parameters, 13 had a negative impact due to lockdown. One important finding from the result is, we got a "negative" impact for all seven positive behavioral parameters in our study. We conclude that lockdown had a more serious impact on the "positive behavioral parameter" than the "negative behavioral parameter". Besides, among the four groups of 30 behavioral parameters, due to the lockdown of COVID-19, the test group children have been more suffering in the "Social Interaction" group. Table 3 found that three behavioral parameters out of 3 (100%) parameters have been negatively affected by the COVID-19 lockdown period. We have chosen and shown graphically (in Fig. 2 ) four "negative behavioral parameters" where we can observe the negative impact. In Fig. 2 , we have marked the negative impact of the red box during the lockdown time. www.nature.com/scientificreports/ Because of the assistance provided by mCARE and more care time given by the family member, we saw positive changes in study participants' behavioral parameters even in the lockdown period. We have selected four behavioral parameters and represent the positive changes (marked by green box) graphically in Fig. 3 . mCARE contribution in COVID-19 lockdown time. During the lockdown period due to COVID-19, the clinical coordinator could continue to give therapy to study participants based on the data from mCARE. For example, Table 4 shows the clinical professional's treatment process in the lockdown period based on the study participant's condition. In this table, we categorize the parameter into four types: communication, daily living skills, motor skills, and socialization.

Based on the children with ASD behavioral data through mCARE: APP or mCARE: SMS, the clinical coordinator could make the proper evidence-based decision for each child with ASD during the lockdown.

Though several works 45, 46 in this relevant area to determine the mental health of the children with ASD in Bangladesh, in this study, we specifically identify the behavioral changes of the children with ASD in Bangladesh during the COVID-19 lockdown period. Compared to other studies, in this work, we have deployed our developed mCARE: APP and mCARE: SMS tools among our cohort. Besides that, our clinical coordinator also conducted the FGD to validate the cohort's data and feedback.

The strong cohort of 300 children with ASD ("Test Group" and "Control Group") with their parent's support and the value-sensitive design-based mobile application (mCARE: APP and mCARE: SMS) is the main strength of our study. As the behavioral development of children with ASD is a long-term process, we faced the challenge of keeping the parents' motivation for providing the data regularly in this study. For this reason, we failed to maintain the same data collection rate (details in Fig. 1 ) from our study population over the study. In this study, we only considered the "test group" of our population with no "Gender-Specific" findings. And we have used our self-created 30 behavioral parameters, which had not been evaluated by standard instruments.

In conclusion, this study indicates that the ongoing COVID-19 pandemic and the subsequent lockdown have undoubtedly produced a mental impact on young children with ASD and their surrounding caregiver personnel. In our research, we found the daily routine change, to be bound in the home for a long time, closed mental health centers for an extended period, the increase of difficulties and mental pressure of parents are the main critical factors underlying behavioral problems of children with ASD due to the COVID-19 outbreak. In the confinement period, children with ASD mainly suffered in their positive behavioral parameters like participating www.nature.com/scientificreports/ in-game, understanding their daily routine, intense interest in toys or dolls, and making social interaction or response. On the other hand, the effectiveness of in-home care and the potential use of online intervention tools like mCARE played a vital role in developing children with ASD at any time, both in normal or any pandemic time in the future. 

Psychosocial and behavioral impact of COVID-19 in autism spectrum disorder: An online parent survey

WHO declares COVID-19 a pandemic

Coronavirus Cases

World Health Organization. WHO Coronavirus (COVID-19) Dashboard (World Health Organization

Tracking Covid-19's Global Spread (Health Care

Early transmission dynamics in Wuhan, China, of novel coronavirus-infected pneumonia

Case-fatality rate and characteristics of patients dying in relation to COVID-19 in Italy

Impact of COVID-19 and lockdown on mental health of children and adolescents: A narrative review with recommendations

The World in Lockdown in Maps and Charts

A qualitative study of child and adolescent mental health during the COVID-19 pandemic in Ireland

United Nations Sustainable Development Group (United Nations

290 Million Students Out of School Due to COVID-19: UNESCO Releases First Global Numbers and Mobilizes Response

Rapid systematic review: the impact of social isolation and loneliness on the mental health of children and adolescents in the context of COVID-19

The potential impact of the COVID-19 pandemic on child growth and development: A systematic review

Policy Responses to Coronavirus (COVID-19): Combatting COVID-19's Effect on Children

American Psychiatric Association. Diagnostic and statistical manual of mental disorders: DSM-5

The relationship between chronotype, sleep, and autism symptom severity in children with ASD in COVID-19 home confinement period

An overview of autism spectrum disorder, heterogeneity and treatment options

Patients with mental health disorders in the COVID-19 epidemic

Detecting developmental delay and autism through machine learning models using home videos of Bangladeshi children: Development and validation study

Autistic disturbances of affective contact

Real-world executive functions in adults with autism spectrum disorder: Profiles of impairment and associations with adaptive functioning and co-morbid anxiety and depression

COVID-19 and "natural" experiments arising from physical distancing: A hypothetical case study from chronobiology

An evaluation of the quality of research on evidence-based practices for daily living skills for individuals with autism spectrum disorder

Immediate psychological effects of the COVID-19 quarantine in youth from Italy and Spain

Lockdowns Compared: Tracking Governments' Coronavirus Responses

The emotional impact of Coronavirus 2019-nCoV (new Coronavirus disease)

Impact of COVID-19 pandemic on the mental health of children in Bangladesh: A cross-sectional study

American Psychiatric Association. Diagnostic and Statistical Manual of Mental Disorders (DSM-5®)

Stress and emotional wellbeing of parents due to change in routine for children with autism spectrum disorder (ASD) at home during COVID-19 pandemic in Saudi Arabia

Parent-reported behavioural changes in children with autism spectrum disorder during the COVID-19 lockdown in Italy

Psychological and behavioral impact of lockdown and quarantine measures for COVID-19 pandemic on children, adolescents and caregivers: A systematic review and meta-analysis

Grant report on mCARE: Mobile-based care for children with autism spectrum disorder (ASD) for low-and middle-income countries (LMICs)

IEEE 44th Annual Computers, Software, and Applications Conference (COMPSAC)

Bangladesh Confirms Its First Three Cases of Coronavirus

Paired t test

National Institutes of Health (NIH)

National Institute of Mental Health and Hospital

Institute For Peadiatric Neurodisorder And Autism in BSMMU

Accessed 10

Median, Mode and Range

Implementing a mental health care program and home-based training for mothers of children with autism spectrum disorder in an urban population in Bangladesh: Protocol for a feasibility assessment study

Parent mediated intervention programmes for children and adolescents with neurodevelopmental disorders in South Asia: A systematic review

This study has been partially supported by an NIH Grant (1R21MH116726-01). The funding source has been used for study design, data collection, analysis of the data and data interpretation, writing this report, and deciding to submit the paper for publication. All authors of the paper have full access to the data of this study and accept the responsibility to submit it for publication. The authors are thankful to four Bangladeshi specialist Autism health care and institutions: The Institute of Pediatric Neuro-disorder & Autism (IPNA) Bangladesh, The National Institute of Mental Health (NIMH), Autism Welfare Foundation (AWF), and Nishpap Autism Foundation and their respective departments for the continuous support throughout this study.

All authors have equal contributions to this study.

The authors declare no competing interests.

The online version contains supplementary material available at https:// doi. org/ 10. 1038/ s41598-021-00793-7.Correspondence and requests for materials should be addressed to M.R.Reprints and permissions information is available at www.nature.com/reprints.Publisher's note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/.