key: cord-0965030-0ve09epq authors: Loftus, Michael J.; Young-Sharma, Tracey E.M.W.; Wati, Shitanjni; Badoordeen, Gnei Z.; Blakeway, Luke V.; Byers, Sally M.H.; Cheng, Allen C.; Jenney, Adam W.J.; Naidu, Ravi; Prasad, Amitesh; Prasad, Vinita; Tudravu, Litia; Vakatawa, Timoci; van Gorp, Elke; Wisniewski, Jessica A.; Rafai, Eric; Stewardson, Andrew J.; Peleg, Anton Y. title: Epidemiology, antimicrobial resistance and outcomes of Staphylococcus aureus bacteraemia in a tertiary hospital in Fiji: A prospective cohort study date: 2022-03-28 journal: Lancet Reg Health West Pac DOI: 10.1016/j.lanwpc.2022.100438 sha: 60bf586271375991a21eb995c021af9ec7beaf95 doc_id: 965030 cord_uid: 0ve09epq BACKGROUND: Staphylococcus aureus bacteraemia (SAB) is one of the commonest bloodstream infections globally and is associated with a high mortality rate. Most published data comes from temperate, high-income countries. We describe the clinical epidemiology, microbiology, management and outcomes of patients with SAB treated in a tropical, middle-income setting at Fiji's largest hospital. METHODS: A prospective, observational study was performed of consecutive SAB cases admitted to Colonial War Memorial Hospital (CWMH) in Suva, between July 2020 and February 2021. Detailed demographic, clinical and microbiological data were collected, including the key outcome of in-patient mortality. To estimate the population incidence, all SAB cases diagnosed at the CWMH laboratory were included – even if not admitted to CWMH – with the population of Fiji's Central Division used as the denominator. FINDINGS: A total of 176 cases of SAB were detected over eight-months, which equated to an incidence of 68.8 cases per 100,000 population per year. Of these, 95 cases were admitted to CWMH within 48 h of index culture. Approximately 8.4% (8/95) of admitted cases were caused by methicillin-resistant Staphylococcus aureus (MRSA). All cause in-patient mortality was 25.3%, increasing to 55% among patients aged 60 or older. INTERPRETATION: This reported incidence of SAB in central Fiji is one of the highest in the world. SAB was associated with significant mortality, especially in those over 60 years of age, despite a relatively low frequency of methicillin resistance. FUNDING: Supported by the National Health and Medical Research Council (Australia) and the GRAM (Global Research on Antimicrobial Resistance) Project, Oxford University (United Kingdom). Background Staphylococcus aureus bacteraemia (SAB) is one of the commonest bloodstream infections globally and is associated with a high mortality rate. Most published data comes from temperate, high-income countries. We describe the clinical epidemiology, microbiology, management and outcomes of patients with SAB treated in a tropical, middle-income setting at Fiji's largest hospital. Methods A prospective, observational study was performed of consecutive SAB cases admitted to Colonial War Memorial Hospital (CWMH) in Suva, between July 2020 and February 2021. Detailed demographic, clinical and microbiological data were collected, including the key outcome of in-patient mortality. To estimate the population incidence, all SAB cases diagnosed at the CWMH laboratory were included − even if not admitted to CWMH − with the population of Fiji's Central Division used as the denominator. Findings A total of 176 cases of SAB were detected over eight-months, which equated to an incidence of 68.8 cases per 100,000 population per year. Of these, 95 cases were admitted to CWMH within 48 h of index culture. Approximately 8.4% (8/95) of admitted cases were caused by methicillin-resistant Staphylococcus aureus (MRSA). All cause in-patient mortality was 25.3%, increasing to 55% among patients aged 60 or older. Interpretation This reported incidence of SAB in central Fiji is one of the highest in the world. SAB was associated with significant mortality, especially in those over 60 years of age, despite a relatively low frequency of methicillin resistance. Staphylococcus aureus bacteraemia (SAB) is one of the commonest bloodstream infections worldwide. 1 The allcause mortality rate from SAB in high-income countries has been reported to be up to 20−30%, 2,3 with even higher rates of over 50% seen in some low-and middleincome countries (LMICs). 4, 5 S. aureus infections − including SAB − are of great importance in the fields of both antimicrobial resistance (AMR) and healthcare-associated infections (HAIs). Regarding AMR, methicillin-resistant S. aureus (MRSA) has been identified as a High Priority Pathogen by the World Health Organization. 6 Antibiotic treatment options for MRSA are more limited and may not always be available throughout LMIC settings. Until recently there has been a paucity of data on MRSA prevalence from LMICs, 7 a situation that is slowly improving due to initiatives such as the Global Antimicrobial Resistance and Use Surveillance System (GLASS). 8 Regarding HAIs, S. aureus is the most frequent cause of healthcare-associated bacteraemia in both high-income 9 and LMIC settings. 10 Healthcare-associated SAB (HA-SAB) has been used in surveillance programs as a marker of healthcare quality, 11 in part as there are specific interventions − such as hand hygiene 12 and lineinsertion bundles 13 − that are proven to reduce the rates of HA-SAB, including in LMICs. 14, 15 SAB is of particular importance to tropical regions such as the Pacific Islands. This region experiences high rates of skin disease (including impetigo, scabies and dermatophyte infections) 16 and diabetes 17 − both key risk factors for community-acquired SAB. A previous study of SAB from Fiji over a decade ago demonstrated a very high annual incidence of 50 cases per 100,000 population. 18 Currently, most published data on SAB comes from high-income, temperate countries. There is little available data from low-and middle-income tropical countries, 19, 20 despite the majority of the global population residing in such settings. To address this knowledge gap, we describe the clinical epidemiology, microbiology, management and outcomes of patients with SAB treated at Fiji's largest hospital. We conducted a prospective, observational study of SAB among inpatients at the Colonial War Memorial Hospital (CWMH) in Suva, over eight months from July 2020 until February 2021. CWMH is a 500-bed tertiary hospital and the largest healthcare facility in Fiji. It serves a local catchment population of Fiji's Central Division (almost 400,000 people) and is also the national referral centre for Fiji's other two divisional hospitals. It has adult, paediatric and neonatal intensive care units (ICUs), and offers specialty services including cardiology, nephrology, orthopaedics, plastic surgery, urology and neurosurgery. CWMH has a six-member Infection Prevention and Control team that conducts regular surveillance of select HAIs. Alcohol-based handrub is available on all CWMH wards, and is present at each bedside within ICUs. A recent point-prevalence survey of HAIs at CWMH reported that 8.7% of inpatients had an HAI, 69.4% had a peripheral vascular catheter and 5.5% had a central vascular catheter. 21 Patients with SAB were eligible if they were admitted to CWMH at the time of blood culture collection, or up to 48 h following blood culture collection. Patients were to be excluded if they were not being treated with curative intent for the entire calendar day on which the index blood culture was collected. We identified patients with SAB by screening new positive blood culture results each day. The CWMH Evidence before this study Staphylococcus aureus bacteraemia (SAB) is a common cause of bloodstream infection and associated with high mortality. However, most knowledge about the epidemiology and outcomes of SAB comes from temperate, high-income countries. The only previous study of SAB from the Pacific Islands region was conducted over a decade ago in Fiji − this reported an incidence of 50 cases per 100,000 population per year however no outcome data was available. We analysed all cases of SAB from the largest hospital of a major Pacific Island Country (Fiji), and found an annualised incidence of 69 cases per 100,000 population. Among those admitted to CWMH, the rate of MRSA was low at only 8.4%, almost half of all adults had diabetes, and in-hospital mortality was over 25%. Healthcare-associated infections were responsible for almost one-third of SAB cases (n = 28, 29%), and are therefore potentially preventable. Our study demonstrates that central Fiji has one of the highest reported rates of SAB in the world, and is associated with significant in-hospital mortality that is greater than high-income countries but comparable to equivalent estimates from other low-and middle-income countries. Efforts to reduce SAB in Fiji could focus on diabetes and skin disease management at the community level, and prevention of healthcare-associated infections at the hospital level. Articles laboratory receives clinical samples not only from CWMH inpatients, but also from nearly every other healthcare centre in Fiji's Central Division. We noted all SAB results in order to estimate a local population incidence, but only collected clinical data for CWMH inpatients. Ward-based data collectors then determined whether patients with SAB were eligible. The notes of eligible patients were reviewed on the wards or in medical records as soon as practicable. Study data were collected and managed using RED-Cap (Research Electronic Data Capture) tools hosted at Monash University, Australia. 22 Clinical and antibiotic data were collected at the initial chart review. Clinical data used definitions from the WHO GLASS Attributable Mortality of AMR Bloodstream Infections document, 23 unless specified otherwise below and outlined in greater detail in the Supplementary Appendix Table S1 . Clinical data included patient age, sex, admission date, admission source, admitting specialty, age-adjusted Charlson comorbidity index (CCI) score, Pitt bacteraemia score and highrisk quick Sequential Organ Failure Assessment (qSOFA) score at SAB onset, and whether the patient had any immunosuppression, hospitalisation, ICU admission, surgical procedure or antibiotic exposure preceding their SAB. Diverging from the WHO GLASS document, we also collected data on the likely source of infection and the epidemiological attribution of their SAB (using the categories of Hospital Acquired, Community Acquired, or Healthcare Associated 24 ). Antibiotic data included all antibiotics received between Days 0 to 2 inclusive ('initial antibiotic therapy'; Day 0 = day of positive blood culture), and the antibiotic susceptibility profile as reported by the Suva lab was also obtained. As the chart review occurred at one time point only, shortly after the diagnosis of SAB, we were unable to collect data on the duration of antibiotic therapy. Effective initial antibiotic therapy was defined as receipt of at least one agent listed in Fiji's Therapeutic Guidelines: 25 for methicillin-susceptible S. aureus (MSSA), intravenous (IV) (flu)cloxacillin or cephazolin; for MRSA, IV vancomycin. Broad-spectrum beta-lactams with anti-staphylococcal activity (such as piperacillin-tazobactam) were also counted as effective against MSSA. Chloramphenicol is commonly used in Fiji to treat non-invasive S. aureus infections, however we deemed chloramphenicol 'ineffective' therapy as it is not a recommended agent for SAB. 25 Data on repeat blood cultures, echocardiography results and patients' discharge date and status were collected retrospectively after patients had either been discharged or died, these data did not require further review of the paper medical record. Any patients discharged home to die had their discharge status recorded as moribund. During data analysis, moribund patients were grouped with those who had died in hospital. Patients' clinical management was entirely at the discretion of their CWMH clinicians, with no input from the research team. There were no specific study interventions, such as mandated echocardiography or repeat blood cultures. Initial identification and antibiotic susceptibility testing (AST) for S. aureus were performed by the CMWH laboratory as per their standard procedures. Clinical samples were plated onto 5% sheep blood agar. Identification was performed using the deoxyribonuclease test and the staphylococcus latex agglutination test, and AST was performed for first-line laboratory antimicrobials using the disk-diffusion method with CLSI breakpoints. 26 Alternatively, if an isolate was either identified as MRSA or was growing repeatedly from blood cultures, identification and AST were performed using the VITEK-2 GP ID and VITEK-2 AST cards (bio-M erieux, Marcy-Etoile, France; version 9.01), respectively. Where possible, the index S. aureus isolate from each patient was sub-cultured, stored at -80⁰C, and shipped to Melbourne, Australia for confirmatory testing. Confirmatory identification was performed using MALDI-TOF (Bruker, Hanau, Germany) and confirmatory AST was performed using VITEK-2 AST cards (bioM erieux, version 8.01) on all available isolates. Echocardiography was performed at the discretion of treating clinicians. Adult transthoracic echocardiograms (TTEs) are performed by dedicated technicians, whereas for children all TTEs are performed by paediatricians trained in echocardiography. There is no access to transoesophageal echocardiography. To calculate the community incidence of SAB, we used the population of the Central Division of Fiji from the most recent 2017 National Population and Housing Census. 27 This was corrected for subsequent population growth using estimates published by the Pacific Community Statistics for Development Division 28 to arrive at mid-year population estimates for 2020 and 2021. To calculate an annualised incidence of SAB, the denominator of person-months was first obtained by multiplying each year's population by however many months of surveillance took place within that year (six in 2020, and two in 2021) and then summing these totals. For the numerator, we excluded any SABs among non-residents of Central Division. The remaining SABs detected at CWMH over the study period were then divided by the number of person-months and multiplied by 12 to determine the annualised incidence of SAB. To calculate the rate of healthcare-associated SABs per 10,000 patient days, we assumed full occupancy of CWMH over the 8-month study period, as hospital occupancy and patient-day data were not available. While this assumption could lead to an inflated denominator and underestimation of the true rate, it is offset by the fact that CWMH is regularly operating at, or slightly above, its stated bed capacity. Data analysis was performed using R (version 3.6.1). In bivariate comparisons, we used Fisher's exact test for categorical variables and Mann−Whitney U test for numeric variables. The Wilson method was used to calculate confidence intervals for proportions. For logistic regression analysis, Directed Acyclic Graphs (DAGs) were employed to identify a minimally sufficient adjustment set of variables to control confounding (Supplementary Appendix Fig. S1 ). The two relationships of interest were the impact of methicillin-resistance on mortality, and the impact of effective initial therapy on mortality. This second relationship was only assessed within the MSSA cohort, as effective therapy is on the causal pathway between AMR status and mortality. Due to concerns regarding over-parameterisation, Akike's information criterion was used to assess the impact of additional variables on model fit. The manuscript has been prepared according to the STROBE checklist. Ethics approval was provided by the Alfred Hospital Ethics Committee (593/19) and the Fiji National Health Research Ethics Review Committee (88/2019). The funders had no role in the design, data collection, analysis, and interpretation, or preparation of this manuscript. A total of 183 consecutive cases of SAB were identified at the CWMH laboratory during the study period, of which, 99 (54%) were associated with a patient admission to CWMH within 48 h of their index blood culture ( Figure 1 ). 86% (85/99) of patients had an isolate available for confirmatory testing in Australia − four isolates were identified as coagulase-negative staphylococci (CoNS) and these patients were subsequently excluded from the study, leaving a total of 95 inpatients for analysis. Confirmatory AST was performed on all available isolates and found only one discrepancy − a patient with MRSA with that was initially identified in Suva as MSSA. Excluding the four cases of CoNS identified on confirmatory testing, and three patients who did not reside in Fiji's Central Division, there were 176 separate instances of SAB detected at the CMWH laboratory over the eight-month study period. This equated to an annualised incidence of SAB for Fiji's Central Division of 68.8 cases per 100,000 population. Regarding HA-SAB, less than one-third of all SABs (28/95, 29.5%) were healthcare-associated (either hospital-acquired, or healthcareassociated but community-onset). This equates to a rate of HA-SAB of 2.3 per 10,000 patient days at CWMH across the study period. Among the cohort of 95 inpatients with detailed clinical data, the median patient age was 44 years (IQR 16 − 59 years), with a range of 9 days to 89 years ( Table 1) . There were 25 (26%) paediatric patients <18 years, including two (2.1%) neonates <28 days (a full description of the paediatric cohort can be found in Supplementary Appendix Table S2 ). The median CCI score was 2, and the median Pitt Bacteraemia score was 0. The prevalence of diabetes among adult patients was 34/70 (48.6%). No paediatric patients had diabetes. All patients were being treated with curative intent for the entire calendar day of their index blood culture. MRSA was responsible for 8/95 (8.4%) cases of SAB, all of which occurred in adults. Just over two-thirds of all admitted SABs (67/95, 71%) were community-acquired. While only 6% (4/67) of community-acquired SABs were caused by MRSA, this rose to 14% (4/28) among healthcare-associated infections, although this was not statistically significant (p = 0.22). Almost half of all SABs (39/95, 41%) were defined as primary infections, with a further two as central-line associated and the remaining 54 as secondary infections ( Table 1) . Skin and soft tissue (40/54, 74%) and bone and joint (9/54, 17%) sources accounted for almost all secondary infections. Overall, 219 blood cultures were collected; ≥1 repeat blood culture was performed in 77/95 (81.1%) patients and 33/95 (34.7%) patients had ≥2 repeat blood cultures. There was variability in the time to first repeat blood culture, with a median of 4 days (IQR 3-6 days) but ranging from 0 to 16 days. Of the 77 patients with at least one repeat blood culture, a negative blood culture was achieved in 73 (94.8%). The remaining four patients all died before clearing their blood cultures. The median time to first negative blood culture was 6 days (IQR 4−8 days, range 0−17), and the median number of further blood cultures until first negative was 1 (IQR 1−2, range 1−5). The longest duration of bacteraemia was ten days and the most positive blood cultures for a single patient was five, across a nine-day period. The antibiotic susceptibility seen among the SAB isolates is summarised in Table 2 . Resistance to penicillin among MSSA isolates was almost universal, with only 4/73 (5.5%) remaining susceptible. All tested MSSA isolates were susceptible to clindamycin and tetracycline, with low (≤10%) rates of resistance seen to ciprofloxacin and trimethoprim-sulfamethoxazole (TMP-SMX). Of note, all MRSA isolates remained susceptible to vancomycin, clindamycin, and TMP-SMX. The 95 patients were prescribed 247 antibiotics as part of their initial therapy − a time when blood culture results were generally not known − with a median of 3 antibiotics each (IQR 2−3, range 0−5). Overall, 71/95 (74.7%) patients received at least one effective antibiotic in their initial regimen. No patients with MRSA received effective initial antibiotic therapy, compared with 71/87 (81.6%) of those with MSSA. The rates of effective initial therapy were similar between paediatric (20/25, 80%) and adult (51/70, 72.9%) cases. The antibiotics most commonly used as part of initial antibiotic therapy were cloxacillin (n = 73, 76.8% of patients), gentamicin (53, 55.8%), ceftriaxone (37, 38.9%) and metronidazole (30, 31.6%). Only five patients received vancomycin as part of their initial treatment regimen, all of whom had MSSA infection and also received empiric cloxacillin. Echocardiography status was determined for 91 patients (95.8%). Within this assessable cohort, a total of 62 echocardiograms were performed on 55 patients (55/ 91 = 60.4%). There was a median of 5 days (IQR 4−7 days) between a patient's index blood culture and their first echocardiogram. Of the 36 patients who did not undergo echocardiography, 15 (n = 2), tricuspid (n = 1), as well as one further case with a vegetation seen on the interatrial septum. All patients had definite endocarditis according to modified Duke criteria, 29 and all survived with a median hospital length of stay of 34 days. The overall in-hospital mortality rate was 25.3% (24/95 The crude in-hospital mortality rate increased with age (Figure 2) , reaching 55.0% (11/20) among those aged 60 years or older, compared to only 17.3% (13/75) among the remainder. Overall, in-hospital mortality among all adults was 31.4% (22/70), versus 8.0% (2/25) among children <18 years (Relative risk 1.34, 95% CI 1.06-1.64, p = 0.032). Both paediatric deaths were in patients <12 months of age but neither were neonates. When assessing the relationship between MRSA and mortality using multivariable logistic regression, ageadjusted CCI score was identified as a key confounder on DAG. MRSA was not associated with significantly increased odds of dying in hospital (OR 1.32, p = 0.73), however higher CCI score was associated with increased mortality (OR 1.52, p = 0.001). Among the MSSA cohort, when assessing the impact of effective initial therapy on mortality, high Pitt score (≥4) was deemed a key confounder on DAG. Effective initial therapy did not significantly alter the odds of in-hospital mortality (OR 3.18, p = 0.21), whereas high Pitt score was associated with increased odds of dying in hospital (OR 17.1, p = 0.0002). For both models above, there was evidence of over-parameterisation when further potential confounders were added. Our research demonstrates that central Fiji has one of the highest reported incidence rates of SAB globally (68.8 cases per 100,000 population per year), which is associated with a significant in-hospital mortality rate over 25%. Globally, most estimates of the population incidence of SAB come from high-income countries, ranging from 15 to 40 cases per 100,000 person-years. 30, 31 Estimates from LMICs have been far less frequent, often focus exclusively on paediatric populations, and potentially suffer from under ascertainment due to lower rates of microbiological testing. 32 A Thai study of all age groups reported a SAB incidence of 7.3 per 100,000 person-years in two rural provinces, 33 while two African studies in children reported incidences of 27 and 48 cases per 100 000 person-years in Kenya and Mozambique, respectively. 34, 35 Our observed rate of 68.8 cases per 100 000 person-years is higher than the 50.1 per 100 000 person-years reported by an earlier study of SAB cases in Suva over 2006−2007, 18 and reinforces that Fiji has one of the highest global rates of SAB. This is likely driven by Fiji's high rates of both diabetes (almost half of all adults in our study) and concomitant skin diseases. 16 One of the closest reported rates of SAB from the literature comes from Northern Australia, another tropical setting, with an incidence of 65 per 100,000 person-years among a cohort with high rates of diabetes (21.7%) and socioeconomic disadvantage. 36 We determined a healthcare-associated SAB (HA-SAB) rate at CWMH of 2.3 cases per 10,000 patient days. HA-SAB is one of the commonest causes of nosocomial bacteraemia 37 and is associated with high mortality, however there are proven interventions − such as hand hygiene, or improved management of intravascular catheters − that can lower the HA-SAB rate. 38 Although a meta-analysis suggested that healthcareassociated infections (HAIs) are 2−3 times more common in LMICs compared to high-income countries, 10 a recent point-prevalence survey of HAIs in Fiji found a relatively low overall prevalence of HAIs at CMWH of 8.7%, 21 slightly below estimates from similar studies in both Australia 39 and Singapore. 40 Despite this apparent low overall rate of HAIs, CWMH's HA-SAB rate of 2.3 per 10,000 patients days is higher than that seen in equivalent large hospitals in Australia (0.96 per 10,000 patient days) 41 or in England (1.04 per 10,000 patient days). 42 HA-SABs may represent a prime candidate for proven, targeted, infection prevention and control interventions at CWMH. We observed an all-cause in-hospital mortality rate of 25.3% for all SABs in Fiji, rising to 31.4% among adult patients with SAB. This is slightly higher than rates of around 20% reported by high-income countries, 2, 43 however is similar to most other studies from LMICs which range from 27 to 33%. 5, 44 Interestingly, a case series from Thailand − which might have been expected to provide the closest comparison to Fiji as another tropical, upper-middle-income country − reported a far higher proportion of patients (40%) either dying in-hospital or being discharged moribund, and a 12-week allcause mortality rate over 50%. 4 Our finding of a significantly increased risk of death with rising age is consistent with the published literature from both highincome and LMIC settings. 4, 43 While the proportion of MRSA isolates in our study was relatively low at 8.4%, this is higher than the rate of only 2.3% from 128 consecutive SABs at CWMH 15 years ago. 18 Reported rates of MRSA vary widely between Pacific Island countries, from very low rates of 0% and 12% in Vanuatu and Cook Islands, respectively, 45, 46 through to 30−42% in Kiribati, Tonga and Samoa. 47−49 Patients with MRSA bacteraemia experienced a higher − but not statistically significant − crude mortality. This may be due to none of these patients receiving effective empiric antibiotics. Because of low numbers of MRSA cases we could not perform logistic regression to correct for confounders associated with both methicillin-resistance and mortality. We note that other studies from LMICs have found conflicting results regarding the impact of ineffective empiric therapy for S. aureus: in Thailand ineffective initial therapy was significantly associated with increased mortality on logistic regression, 4 whereas such an association was not evident in a large multinational study across South America. 50 Just over half of our assessable cohort underwent an echocardiogram; endocarditis was seen infrequently (7.3%, n = 4) with none of these patients dying. This contrasts with reported rates of endocarditis following SAB of up to 25%, 51 with accompanying in-hospital mortality of 20−30%. 52 Our prevalence could be falsely low in part due to the absence of transoesophageal echocardiography at CWMH, which has a higher sensitivity. Additionally, the low observed prevalence and mortality of endocarditis may be related to survivor bias, with healthier patients more likely to survive long enough to undergo an echocardiogram (a median of 5 days after their index culture). Other cohort studies from LMICs have demonstrated similarly low rates of endocarditis following SAB − between 7 and 14% − with underascertainment felt to be likely. 4, 5 Although our research was conducted during the coronavirus disease 2019 (COVID-19) pandemic, across the entire study period there was no community transmission of SARS-CoV-2 in Fiji and very few restrictions beyond the closure of the international border. We therefore feel our findings are representative of the outcomes and incidence of SAB during a non-pandemic period. There are some limitations to our research. First, this single-centre analysis was from Fiji's largest hospital, located in the Central Division, and may not be representative of other hospitals or Divisions in Fiji. We do not have clinical or outcome data on SABs who were not admitted to CWMH within 48 h of their index culture, so cannot assess how this cohort might differ from the inpatient cohort presented above. Second, for pragmatic reasons, chart review occurred at a single time point shortly after index blood culture − this precluded data collection on antibiotic treatment duration, procedures for source control or presence of metastatic infection. All of these are important factors in determining SAB outcome, which could not subsequently be corrected for in our analyses. Third, our multivariable regression should be interpreted with caution as we were unable to include all variables suggested by the DAG process, due to small sample size and concerns regarding over-parameterisation. Finally, our measurement of SAB incidence may be a slight overestimate if there were further cases of CoNS incorrectly identified as S. aureus among patients with no isolates for confirmatory testing. However, conversely, our measurement could instead be an underestimate if there were further SAB cases that went undiagnosed, were misdiagnosed as CoNS, or were detected by one of the few small microbiology laboratories in Central Division outside of CWMH. Conducting detailed, prospective, clinical and laboratory research in low-and middle-income countries is challenging, particularly during a global pandemic. Despite the limitations outlined above, our research contributes valuable new knowledge about SAB from a tropical, middle-income country. We have shown that central Fiji has one of the highest rates of SAB in the world. Based on the existing literature and our own new research, we believe efforts to reduce SAB in Fiji should focus on the prevention of both diabetes and skin diseases at the community level, and the optimisation of infection prevention activities at the hospital level. MJL, AJS, AWJJ, AYC, RN, ER and AYP conceived, designed and initiated study. MJL, AJS and AYC performed statistical analysis. ER, RN, LT, AP, TV, and VP provided technical and logistical advice. RN and LT coordinated and supervised the Fiji study staff performing data collection. TY-S and SW performed ward data collection. AP, TV and VP undertook all Suva laboratory work and data collection. JW, LB, SB, EvG and ZB undertook all Melbourne laboratory work and data collection. MJL wrote the first draft, prepared figures and tables, and made subsequent revisions based on coauthors' feedback. 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