key: cord-0961740-1kevzpza authors: Eidensohn, Yehuda; Wei, Abraham; Sirkin, Michael; Dever, Lisa L. title: Brevibacteria tibial osteomyelitis date: 2021-01-12 journal: IDCases DOI: 10.1016/j.idcr.2021.e01046 sha: 6098f67a8019733cdea9dee5773ad0b6da9472cc doc_id: 961740 cord_uid: 1kevzpza Brevibacteria are Gram-positive rods found in human skin flora and dairy products. Although generally not considered human pathogens, case reports have implicated Brevibacterium species as rare causes of bacteremia, endocarditis, peritonitis, and osteomyelitis. We report a case of Brevibacterium tibial osteomyelitis in an immunocompetent individual with implanted hardware and highlight the challenge of identifying the organism and recognizing it as a potential pathogen. Brevibacteria are Gram-positive rods that appear as diphtheroid rods in cultures for the first 24 h and then transition to coccoid or coccobacillary morphology [1] . They are found in raw milk and contribute to the taste and scent of cheese. They are normal human skin flora and are thought to contribute to foot odor. We report a case of tibial osteomyelitis associated with implanted hardware in an immunocompetent adult due to this organism. A 40-year-old man with a history of extensive lower extremity trauma two years prior requiring left leg open reduction and internal fixation presented to the emergency department with left ankle pain and serous drainage for two months. He had been treated previously with oral cephalexin with no response. He was afebrile and his vital signs were within normal limits. Physical examination was remarkable only for a 0.5 cm open wound over the left medial malleolus. There was no drainage, erythema or swelling noted. Laboratory studies were performed including complete blood count, complete metabolic panel, erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and procalcitonin. All were within normal limits. Plain radiograph of the left ankle showed a healed fracture of the distal tibia with intact plate and screws. There were no acute changes. He was discharged with wound care instructions. In outpatient follow-up one week later, he was found to have expansion of the left ankle wound with profuse serous drainage concerning for underlying osteomyelitis. He underwent incision and drainage of the left distal tibia. Surgical exploration revealed a purulent sinus tract from the skin to the implanted plate and screws. All hardware was removed. Four operative cultures were obtained; two from the wound, one from deep ankle tissue, and one from the periosteum. Post-operatively, he received empiric intravenous (IV) vancomycin 1250 mg every 8 h, cefepime 2 g every 8 h and metronidazole 500 mg every 8 h. Routine laboratory studies were normal with the exception of an elevated CRP that trended from 24 to 3 mg/L and ESR that trended from 9 to 17 mm/hour during the course of his hospitalization. Blood and urine cultures were negative. Two wound cultures and one tissue culture grew coryneform Gram-positive rods initially identified as Corynebacterium species. Two days later, the isolate was confirmed to be Brevibacterium species via biochemical testing (Analytical Profile Index Coryne, bioMerieux, Marcy-l'Etoile, France), but was unable to be identified to the species level. The patient had decreased pain and improved wound healing following surgery. Due to subtherapeutic vancomycin levels despite aggressive dosing, he was changed to IV daptomycin 10 mg/kg (750 mg) every 24 h. He was discharged home to complete a five-week course of therapy. The susceptibility of the Brevibacterium species was not known at the time of discharge. Agar disc diffusion susceptibility testing was not available at the time due to a nationwide shortage of Mueller-Hinton agar during the coronavirus pandemic. The isolate was sent to an external laboratory for susceptibility testing. Susceptibility results returned two weeks later. The isolate was susceptible to erythromycin (MIC < 0.50 mg/mL), gentamicin (MIC < 1.0 mg/mL), penicillin (MIC 0.12 mg/mL), rifampin (MIC < 1.0 mg/mL), tetracycline (MIC < 1.0 mg/mL), and vancomycin (MIC < 2.0 mg/mL). Three weeks following surgery, the patient's left tibial wound was healing and sutures were removed. After 5 weeks of IV daptomycin he was transitioned to oral amoxicillin 500 mg every 8 h for a planned 4 week-course of therapy. Six weeks post-operatively, his surgical wound was well healed with no tenderness or erythema, and ESR and CRP levels were normal. The genus Brevibacterium includes many species but only nine have been isolated from humans: B. linens, B. iodinum, B. epidermidis, B. casei, B. mcbrellneri, B. otitidis, B. paucivorans, B. massiliense and B. sanguinis. Brevibacterium species were thought to be apathogenic until 1991, when the first case of sepsis due to B. epidermidis central line-associated blood stream infection was reported by McCaughey [2] . Since that time, at least 18 total case reports have implicated Brevibacterium species in human disease (Table 1) [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16] [17] [18] [19] [20] [21] . B. casei is the most frequent species isolated from clinical specimens [1] . The most common infection has been bacteremia (n = 10), with the rest of cases being peritonitis, pericarditis, endocarditis, brain abscess, and osteomyelitis. Reported patient characteristics vary widely. Five patients were immunocompromised; two had acquired immunodeficiency syndrome (AIDS) and three were receiving chemotherapy for cancer. Others had a history of cancer, or had other systemic diseases including Zollinger-Ellison, aplastic anemia, pulmonary hypertension, heart failure or methylmalonic acidemia. Presence of foreign material or central venous catheters (CVC) were common. Most cases of bacteremia were associated with CVC. The patient with peritonitis was receiving continuous ambulatory peritoneal dialysis, and the patient with endocarditis had prosthetic valves. The cases of Brevibacterium brain abscess and osteomyelitis occurred in immunocompetent healthy patients with no apparent predisposition [2, 20] . A theme of these case reports is the challenge of recognizing the organism is not a skin contaminant and correctly identifying it. This often requires extensive biochemical testing or sending isolates to a reference laboratory for identification. Simple mass spectrometry is not adequate for identification. Earlier identification techniques such as the API Coryne System (bioMérieux, USA), were reported having discrepancies or errors in identification [10, 18] . Other adjunctive tests include 16s rRNA analysis, fatty acid analysis with gas chromatography, and degradation of casein, tyrosine, and xanthine. Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) using spectra of bacterial peptides is the most reliable newer modality of identification, but is not yet widely available in microbiology laboratories. Antimicrobial susceptibility testing is also a challenge. Techniques most commonly reported are Etest and agar disc diffusion, with only a few successful instances of automated microdilution testing. Even when a MIC value is obtained, no standard breakpoints exist for Brevibacterium. The most commonly used breakpoints were proposed by Funke in 1996 [22] . More recent reports used the Clinical and Laboratory Standards Institute's recently established breakpoints for Corynebacterium [23] . Isolates are universally sensitive to the glycopeptides with occasional betalactam resistance. These are consistent with early work by Funke, and are why the most common empiric therapy has been vancomycin or teicoplanin [22] . We chose to use daptomycin in our patient due to the inability to achieve therapeutic levels of vancomycin despite every 8-h dosing. Brevibacteria can cause serious infections even in immunocompetent individuals, especially in the presence of CVC or implanted hardware. The presence of implanted hardware was likely a predisposing factor in our patient. Our case highlights the need for greater awareness of the potential pathogenicity of Brevibacterium species, especially in patients with implanted material, and the possibility of misidentification as apathogenic coryneform bacteria. None. The patient provided verbal and written consent for publication. Yehuda Eidensohn: Care of patient, writing and editing of manuscript. Abraham Wei: Care of the patient, editing of manuscript. Michael Sirkin: Care of patient and review of manuscript. Lisa L. Dever: Care of patient, writing, editing, submitting and revising manuscript. The authors report no declarations of interest. 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