key: cord-0960575-ai3bxsp4
authors: Ruran, Hana B; Adamkiewicz, Gary; Cunningham, Amparito; Petty, Carter R; Greco, Kimberly F; Gunnlaugsson, Sigfus; Stamatiadis, Natalie; Sierra, Gabriella; Vallarino, Jose; Alvarez, Marty; Hayden, Lystra P; Sheils, Catherine A; Weller, Edie; Phipatanakul, Wanda; Gaffin, Jonathan M
title: Air quality, Environment and Respiratory Outcomes in Bronchopulmonary Dysplasia, the AERO-BPD cohort study: design and adaptation during the SARS-CoV-2 pandemic
date: 2021-06-30
journal: BMJ Open Respir Res
DOI: 10.1136/bmjresp-2021-000915
sha: efb18bdb9fad265a6bfc721f46ac97cbf9fd7f60
doc_id: 960575
cord_uid: ai3bxsp4

INTRODUCTION: Almost half of all school-age children with bronchopulmonary dysplasia (BPD) have asthma-like symptoms and more suffer from lung function deficits. While air pollution and indoor respiratory irritants are known to affect high-risk populations of children, few studies have objectively evaluated environmental contributions to long-term respiratory morbidity in this population. This study aimed to examine the role of indoor environmental exposures on respiratory morbidity in children with BPD. METHODS AND ANALYSIS: The Air quality, Environment and Respiratory Ouctomes in BPD (AERO-BPD) study is a prospective, single-centre observational study that will enrol a unique cohort of 240 children with BPD and carefully characterise participants and their indoor home environmental exposures. Measures of indoor air quality constituents will assess the relationship of nitrogen dioxide (NO(2)), particulate matter (PM(2.5)), nitric oxide (NO), temperature and humidity, as well as dust concentrations of allergens, with concurrently measured respiratory symptoms and lung function. Adaptations to the research protocol due to the SARS-CoV-2 pandemic included remote home environment and participant assessments. ETHICS AND DISSEMINATION: Study protocol was approved by the Boston Children’s Hospital Committee on Clinical Investigation. Dissemination will be in the form of peer-reviewed publications and participant information products. TRIAL REGISTRATION NUMBER: NCT04107701.

Advances in neonatal medicine have resulted in the improved survival of infants born at earlier gestational ages; however, despite these interventions, the rate of bronchopulmonary dysplasia (BPD) has not declined and is one of the most common complications of preterm birth. Chronic lung disease of prematurity is evident in a growing population of children with respiratory health impairment. During the first 2 years of life, there is a 50% risk of readmission if the patient has the diagnosis of BPD. This risk continues with almost half of all school-age children with BPD develop asthma or asthma symptoms. 1 An even greater proportion has poor lung function at school age. [2] [3] [4] [5] [6] Several recent studies associate childhood lung function as the main precursor of adult pulmonary function [7] [8] [9] [10] in birth cohorts and asthmatic populations. The knowledge that indoor air quality is associated with respiratory symptoms, bronchiolitis, healthcare utilisation and impaired lung function in children, particularly in asthmatic populations, is widespread, but children with BPD are largely excluded from asthma epidemiologic and clinical studies and may not have the same response to pollutant exposures. As of now, long-term respiratory research in BPD has focused on neonatal factors, yet neonatal insults only partially explain the later childhood health outcomes. Therefore, determining potentially modifiable environmental contributions to respiratory morbidity and lung function in school-age children with BPD is urgently needed.

Improved neonatal care to premature infants over the past 3 decades increased survivorship without improvement in prevalence of BPD 11 or long-term respiratory morbidity into childhood, leading to a growing population of children with respiratory health problems. 12 13 Two recent preterm birth cohorts from Sweden 14 and Australia 15 demonstrated current respiratory symptoms in 40% and 52% of preterm children Open access at school age, respectively, clearly indicating the rate of long-term respiratory morbidity is high despite improved neonatal care. With approximately 30 000 infants per year born in the USA with birth weights under 1500 g (very low birth weight) or extreme prematurity (<28 weeks' gestation) 16 17 and rates of BPD are estimated at over 40% for these infants, 12 a growing population of at least 12 000 children per year are at high risk of long-term respiratory morbidity. It is estimated that almost half of survivors suffer from asthma or asthma-like symptoms at school age and up to 60% have abnormal lung function.

Debate exists as to whether children with a history of chronic lung disease of prematurity truly develop asthma or if they suffer from a separate asthma-like condition. 18 Children with BPD have similar symptomatology and lung function impairment 2 but lack typical features of childhood asthma-personal or family history of atopy, aeroallergen sensitisation and elevated markers of allergic airway inflammation. Several cohorts have demonstrated normal levels of FeNO in school-age children with BPD, 19 20 significantly lower than comparable patients with asthma, 21 and low levels of aeroallergen sensitivity. 19 Regardless of definition, the rates of respiratory morbidity, whether defined as physician diagnosis of asthma 1 22 23 or as report of current asthma-like symptoms, 11 14 15 are similarly high.

While childhood asthma is known to have strong environmental contributions to both inception and morbidity of disease, little is known about the environmental contributions to respiratory morbidity in children with BPD. This absence of information is due to several phenomena. First, children born prematurely are frequently excluded from large birth cohort studies of allergy and asthma in order to remove subjects with a comorbid respiratory condition. Second, studies of preterm infants have primarily investigated neonatal survival and early life determinants of early life morbidity. In the few studies that analyse factors related to long-term respiratory health, the NICU-centric (neonatal intensive care unit) approach typically only allows for neonatal risk factors to be assessed. Home-based environmental determinants of asthma development in children with BPD have been limited to tobacco smoke exposure, 11 pet ownership and respiratory viral infections 1 in infancy, assessed by parental questionnaire. For non-BPD cohorts, exposure to ambient air pollutants has been associated with asthma development, asthma symptoms and asthma exacerbations. [24] [25] [26] [27] [28] [29] [30] [31] Moreover, home-based measurements of nitrogen dioxide (NO 2 ) and other pollutants with indoor sources have been associated with asthma symptom severity 25 32-34 in children, even at modest levels of exposure. 33 Across many locations, indoor exposure to environmental pollutants has been associated with increased incident asthma diagnosis, 35 asthma symptoms 33 36 and asthma severity 32 in children. The most consistent associations have been with NO 2 , 32 33 36 ozone and PM 2.5 . 37 Studies of healthcare utilisation support the symptomatic findings. Ambient air quality is associated with incidence of emergency department visits and hospitalisations 38 and episodes of bronchiolitis 39 40 in children. In fact, Strickland and colleagues 41 found that associations between ambient pollutant concentrations and paediatric emergency visits for asthma exacerbations were largest among children born preterm, suggesting a uniquely vulnerable population of children. This finding highlights the importance of studying personal exposure to air pollution in this high-risk paediatric population, beyond just those with asthma. We have similarly found that that preterm children in the School Inner City Asthma study are at higher risk for pollution-related health effects than non-preterm children with asthma in the same cohort. 42 Effect modification of preterm gestational age (GA) <37 weeks was seen on the relationship of indoor black carbon (BC) exposure with greater asthma symptoms and a significantly lower forced expiratory volume in one second (FEV 1 ) percent predicted in preterm children than term children with exposure to PM 2.5 . 42 These data suggest preterm children are a subpopulation of asthmatics more highly vulnerable to the effects of poor air quality with increased symptoms and lower lung function. Moreover, these data suggest that findings from childhood asthma environmental health and medical intervention studies cannot be generalised from asthma to BPD. Therefore, investigating air quality and health outcomes specific to the BPD population is important and is currently a substantial knowledge gap.

This study fulfils several unmet needs and knowledge gaps. It will be the first study to objectively evaluate the role of home environmental exposures on school-age children with BPD. This unique study design will pair objective in-home assessment of pollutant and allergen exposures with objective outcome measures of lung function and prospectively assessed symptomatology-something that is currently lacking in the BPD literature.

The motivation for this study is to begin to unravel the basis for the increased prevalence of asthma-like symptoms and lung function abnormalities outside of neonatal events among school-age children with BPD. There are two primary study objectives: 1. Quantify the contributions of home environmental exposures on respiratory morbidity in children with BPD. 2. Determine the effect of indoor air quality on lung function in children with BPD. The study will focus on the role of indoor irritants, while also considering factors such as indoor temperature and humidity, indoor allergens, medical history and family social characteristics. Objective lung function, prospective respiratory symptom assessments and environmental measurements will be made concurrently over two 7-day periods using novel and low burden objective monitoring Open access in 240 children. Atopic status will be characterised and BPD severity as potential effect modifiers. These data will provide the needed information to develop interventions aimed at improving long-term respiratory outcomes in patients with BPD.

Two hundred and forty school-age children with a diagnosis of BPD will be recruited to participate in an epidemiologic cohort study of one clinic-based and two home-based assessments. Measurements will be made to find associations of home environmental exposures with respiratory symptoms and lung function across repeated timepoints, including effect-modifying factors. Longitudinal analyses will explore variation (over 14 days) in respiratory symptom outcomes and pulmonary function in association with changes in environmental exposures, measured in two seasons to address known seasonal variation in both exposure and outcome measures.

Inclusion criteria ► Must be 6-12 years old at the time of enrolment. ► Born <32 weeks' GA. ► Diagnosed with BPD by requiring greater than 28 days of oxygen or other respiratory support (ventilator or continuous positive airway pressure (CPAP)) in the neonatal period.

Exclusion criteria ► History of major airway or chest surgery, such as tracheostomy, open heart surgery, lung surgeries, spinal surgeries (not including patent ductus arteriosis closure). ► Physical or mental impairment that will prevent subject from successfully conducting spirometry. ► Plans to move out of state within the next 9 months.

Families of potentially eligible children will be contacted by letter and phone. A short screening survey will be administered by phone to further ascertain study eligibility. Families who are eligible and interested in the study will be scheduled for a clinic visit at our wellestablished Boston Children's Hospital (BCH) Clinical Research Unit. Round-trip transportation will be arranged to overcome transportation barriers and families will be reimbursed for their time. Reminder phone calls will be made 2 days prior to the visit, when staff will confirm absence of an acute illness over the prior 2 weeks. Children will be asked to refrain from use of certain medications prior to the visit (that interfere with spirometry). The definition of BPD used for enrolment is based on the 2001 National Institute of Child Health and Human Development (NICHD) workshop categorised BPD based on severity, gestational age and postmenstrual age assessment of BPD severity. 43 Acknowledging that the optimal BPD definition has recently been the topic of debate, 44 we selected the 2001 NICHD definition because it was the consensus definition at the time of birth for the majority of children eligible to participate in this study and remains the primary validated 45 consensusbased definition in clinical practice. Confirmation of eligibility is performed by reviewing the subject's birth and neonatal medical records at the time of enrolment.

Clinical, social and physiological data will be collected at the research clinic visit and outcome assessments of symptoms and lung function, as well as environmental data will be collected during two in-home visits, which will consist of a home environmental audit, dust sampling and 7 days of ambient air sampling (see figure 1 ). Staff will be trained, certified and monitored over time in the collection of each measurement, following written procedures, as developed for the SICAS 46 and SICAS-2 47 trials.

The following will be collected at the research clinic visit: standardised questionnaires regarding health, medical history, demographic and family characteristics; objective tests of atopy and lung function; anthropometry; oropharyngeal examination and biological samples (urine, blood). Participants will undergo the following, Figure 1 Schema depicting study timeline for each recruitment year. *Personal outcomes measured at the home sampling visits. Order of 'cold' and 'warm' sampling visits will vary based on enrolment season.

in the order listed: (1) brief orientation to testing facility and informed consent/assent; (2) urine collection; (3) anthropometry, oropharyngeal examination; (4) oscillometry; (5) pre-bronchodilator and post-bronchodilator spirometry; (6) venipuncture; (7) questionnaires.

The child (with parental assistance) will be instructed to void into a collection device.

The child's weight (to 0.1 kg) and standing height (to 0.1 cm) will be measured using a calibrated digital electronic scale and stadiometer, respectively. Measures will be repeated three times, and average values will be used.

Oscillometry will be performed by standard procedure 47 using the Tremoflo device (Thorasys, Montreal, Canada), calibrated daily with test load. After brief orientation, at least reproducible efforts will be recorded. The following parameters will be recorded from the best effort R 5 , R 20 , R 5-20 , f res , AX 5 .

Spirometry will be performed according to ATS (American Thoracic Society) guidelines. 48 At least reproducible flow-volume loops will be obtained using a portable spirometer (Pneumotrac; Morgan Scientific, Haverhill, Massachusetts, USA), calibrated daily, using quality standards 49 and the Global Lung Initiative reference equations. 50 After baseline manoeuvres, 2.5 mg of albuterol will be administered via nebuliser (or 4 inhalations of albuterol 90 mcg by metered dose inhaler with aerochamber, if nebuliser therapy prohibited) and spirometry will be repeated 10-15 min later to obtain postbronchodilator forced vital capacity (FVC), FEV 1 and forced expiratory flow (FEF) . Children will be asked to withhold short-acting bronchodilator medications for 6 hours and long-acting bronchodilator medications for 48 hours prior to testing. Venipuncture A 30 mL venous blood sample will be obtained using paediatric procedures. Within 1 hour, the sample is spun and aliquoted into vials for plasma and cell samples. Samples will be assayed for total immunoglobulin E (IgE) and a panel of common aeroallergens to determine respiratory sensitisation via Phadia ImmunoCAP assay (Thermo Fisher, Uppsala, Sweden). Specific IgE >0.35 kU will be considered positive for sensitisation; children with any positive tests will be considered atopic.

Standardised assessments include those for respiratory symptoms; allergy; medical, family and social history; medications; home and neighbourhood characteristics; frequency of respiratory illnesses/infections; and healthcare utilisation. All instruments will be completed by the parent and child, when appropriate for age, with assistance by research staff as needed. Questionnaires and their relationship to our aims are shown in table 1.

Two in-home sampling visits will be scheduled to occur in a warm season (April-September) and a cold season (October-March), separated by at least 3 months. A twomember research team will visit the family at a convenient time to the family. At the start of each sampling period the team will (1) conduct a home environmental audit; (2) set up an air monitor to measure indoor air quality and thermal conditions (7 days) in the child's bedroom; and (3) instruct the child and parent to complete the daily symptom and household activities diary. Staff members will visit the family at the completion of the 7-day collection period, at which time they will (1) retrieve monitoring devices; (2) collect symptom and activity diaries;

(3) administer questionnaires to capture the primary outcome-maximum symptom days over the prior 2 weeks, as well as updated medical and healthcare utilisation information since the prior encounter; (4) collect a vacuumed dust sample from the bedroom for allergen assays: (5) perform spirometry with a portable spirometer (Pneumotrac; Morgan Scientific, Haverhill, Massachusetts, USA) calibrated daily, using quality standards 49 and the Global Lung Initiative reference equations 50 and oscillometry by Tremoflo device (Thorasys, Montreal, Canada). Staff will contact the family after the first two nights to troubleshoot any problems and reinforce the protocol.

Staff will use a structured checklist completed during a home 'walk through' to characterise the home physical and social environment (15-20 min) . The checklist will be adapted from one developed by Dr Adamkiewicz that includes 100 precoded categories (observed/ not observed: evident mould, pests, kitchen/bathroom ventilation characteristics, combustion sources, chemical exposures). 51

Portable indoor air quality (IAQ) monitors that provide real-time measurements, developed by collaborators at the TH Chan Harvard School of Public Health, 52 will be placed on the child's bedroom shelf away from direct radiator contact and will monitor PM 2.5 , NO 2 ,

NO, carbon dioxide (CO 2 ), temperature and humidity during the 7-day monitoring period. Sensors will output to data loggers every 5 min. We also will measure PM 2.5 mass integrated/averaged over the 7-day monitoring period using standard methods 53 54 and in real time.

NO and NO 2 will be calibrated quarterly and replaced every 18 months, CO 2 will be calibrated annually and all sensors will be assessed for any signal drift. International Study Of Asthma And Allergies In Childhood (ISAAC) asthma core questions: Score of ≥5 on the eightitem global wheezing questions has a sensitivity of 95% and specificity of 100% for identifying asthma 68 69 Composite Asthma Score Index (asthmatic children only, detailed above) 65 Paediatric sleep questionnaire 70 Modified asthma control test 71 Rhinitis ISAAC rhinitis core questions 69 72 73 Respiratory illnesses SICAS healthcare utilisation and respiratory infections (last 12 months) 74 

Dust collection and allergen/endotoxin assays Dust for allergens and endotoxin will be obtained by vacuuming a 2 m 2 area in the child's bedroom plus bedding using an Oreck XL handheld vacuum with a special dust collector (DACI laboratory, JHU) fitted into the inlet hose of the vacuum using a standardised protocol, 55 consisting of vacuuming each surface for 5 min. Samples will be weighed in an environmentally controlled laboratory and extracted. Allergen concentrations will be measured using the MARIA assay 56 (using a Universal Allergen Standard), on a Bio-Plex Bead Suspension Array System that allows simultaneous capture and detection of multiple allergens in a single sample. Endotoxin (which stimulates airway inflammation 57 will be measured by enzyme immunoassay as previously described. 58 Dust samples will be banked appropriately, providing opportunities for future evaluation of the exposome, such as microbiome analyses.

A summary of the key exposures, outcomes, confounders and modifiers is shown (table 1) . Most indices will be analysed as continuous exposures or as quartiles of their distributions; when clinical thresholds exist, indices will be dichotomised.

Analyses will include (1) quantification of associations between home environment exposures and the prevalence and respiratory symptom severity and lung function; (2) assessment of whether the associations between home environmental exposures and respiratory symptoms or lung function are modified by atopy, BPD severity, neonatal medical course (ventilation, length of oxygen exposure) and selected family and neighbourhood exposures. Sensitivity analyses will evaluate the robustness of inferences and will explore variation by BPD severity in stratified analyses. The main analyses under both aims can be unified under the general approach of quantifying repeated measures analyses of associations between home environment exposure (X) and respiratory-related outcome (Y). The primary symptom outcome is maximum symptom days in the prior 2 weeks, adapted from asthma epidemiology and intervention studies for similar age groups 59-62 : maximal number of days with symptoms in the weeks before the visit is defined as the largest value among the following three variables: number of days with wheezing, tightness in the chest or cough; number of nights with disturbed sleep as a result of breathing symptoms; and number of days on which the child had to slow down or discontinue play activities because of breathing symptoms. In the absence of disease-specific validated outcome measures for BPD, we adapted instruments frequently used in asthma epidemiology studies (maximum asthma symptom days, childhood asthma control test) to solicit more general respiratory symptoms that are frequently encountered in this population and overlap with asthma symptoms. While these ask specifically about cough, wheeze and chest tightness, they also solicit information about exertional impairment and sleep disruption from 'breathing issues' more generally. The daily diary completed during the exposure assessment period includes daily questions about cough, wheeze and shortness of breath. The primary lung function outcome for associations with temporally related lung function measure is specifically FEV 1 percent predicted.

Linear (for continuous outcomes) and generalised linear (for count outcomes) models will be used to assess the repeated-measures associations between environment exposures X and respiratory outcomes Y, adjusting for age, sex, race, ethnicity, family income, parent education, season and BPD severity. Other covariates (confounders, mediators; table 1) will also be considered. We will first estimate the association between each individual exposure and respiratory outcome, then assess the overall effect combining multiple environment exposures. Variable selection will be conducted with statistical approaches guided by biological rationale and conceptual considerations. A two-stage approach will be used. First, we will use variable selection techniques based on covariates only, such as cluster analysis or principal component analysis, eliminating variables that are highly correlated to avoid collinearity in subsequent modelling. We will then use cross-validated variable selection techniques to build models involving multiple environment exposures.

Effect modification will be explored using interaction tests and stratified analyses, assessing factors including BPD severity and atopy. BPD severity will be assigned based on consensus guideline 45 and extracted from the primary medical record. Atopy will be defined by having one or more positive specific IgE >0.35 kU to the panel of allergens. We will also examine if associations between specific allergen levels and respiratory outcomes are stronger in those with a specific sensitivity (by IgE) to that allergen.

Secondary outcomes will consider the effect of indoor exposures on annualised healthcare utilisation for urgent respiratory care, annualised number of respiratory illnesses. Additional measures of lung function will be evaluated in relation to indoor air quality exposures: FEV 1 /FVC ratio, and FVC percent predicted; oscillometry measures: R 5 , R 20 , R 5-20 , f res , AX 5 .

The proposed statistical analysis assessing the impact of environmental exposures on respiratory-related outcomes is based on generalised estimating equations to account for correlation among clustered observations at the subject level with assumptions derived from similar studies. 36 42 62 63 Based on these assumptions, a sample of 240 enrolled subjects, a 20 ppb change in NO 2 will Open access provide 89% and 87% power to detect a 1.0 maximum symptom day difference and a 3% FEV 1 percent predicted difference, respectively. A 30 µg/m 3 change in PM 2.5 would provide 99% power to detect an association with either 1.0 maximum symptom day or 3% FEV 1 percent predicted. We do not expect that confounders will significantly reduce our power. Our indoor school data showed that only between 1% and 9% of the variation in exposures was explained by demographic data and season.

Enrolment was halted in early March 2020 due to the SARS-CoV-2 pandemic. Both state and medical centre restrictions on the use of the hospital-based clinical research unit and on the performance of home environmental assessments limited the capability to complete the planned assessments as part of the original protocol. Moreover, participants became reluctant to participate in in-person/in-home clinical research due to concerns over social distancing and risk of attending hospital research clinic visits. In order to maintain the research objectives, study integrity and ensure the safety of the participants and research staff, 64 the protocol was altered to augment recruitment, enable remote enrolment and provide minimal-contact home sampling and health outcome assessment.

In order to broaden our recruitment base, we partnered with local NICU and large paediatric practices to advertise the study and directly recruit from patient rosters. Expanding recruitment to sites outside of Boston Children's Hospital also augments generalisability and minimises potential bias of only recruiting from a tertiary care medical facility.

Given the anticipated local/institutional restrictions for in-person study visit conduct, compounded by likely ongoing participant concerns about coming to the clinical research centre for study visits, approaches were developed to allow performing the informed consent process remotely. Interested participants are mailed/ emailed study information, including the informed consent document, for their pre-review. Following this, study team members use video conferencing software to conduct a visit that allows for a complete discussion of study participation and conduct the informed consent acquisition process, including using electronic signature platforms for participants to sign the consent documents.

During mandatory or preferred times of social distancing, enrolment procedures can be performed remotely. In this situation, all questionnaire assessments will be performed via a virtual meeting. Research laboratory flexibility was expanded to allow labs to be drawn at a remote site affiliated with BCH, limiting the need for participants to come to the main academic medical centre for blood draws. When able, brief visits to obtain objective assessments (anthropomorphic data, spirometry and oscillometry) are performed at the clinical research unit, minimising the time for participants and staff to be in close contact at the hospital.

Adaptations to the home sampling visits allow for complete data collection without research staff entering the home and with minimal in-person interaction. Vacuum dust sampling is performed by providing families with a vacuum outfitted with dust traps for parents to perform the procedure. Written instruction (provided in English and Spanish) and a virtual video interface allow the study staff to guide the family through the procedure. Similarly, curbside IAQ monitor setup is performed by dropping off the monitor at the family's home with written instructions for set-up and a virtual platform used to guide appropriate deployment in the child's bedroom. The in-person home environmental walkthrough inventory was adapted to a virtual visit in which the participant's caregiver assists in the physical evaluation of the home environment either using their own device or a study-provided tablet with video-conferencing capability. The research team guides the walkthrough and evaluation by instructing the caregiver to point the camera at the relevant areas of investigation.

Spirometry and oscillometry are typically performed with the participant performing specific breathing manoeuvres in close proximity to the research staff while the staff actively coaches the research subject through the manoeuvre. Ensuring the integrity of the primary spirometry endpoint is paramount. In an effort to achieve remote collection of FEV 1 measurements, remote spirometry with the ZEPHYR x (https://www. zephyrx. com/) MIR spirometer device was implemented. This is an app-based home spirometry system with live coaching ability (ie, video conferencing embedded in the app so research staff can coach in real time). The device is United States Food and Drug Administration (FDA) approved and security has been vetted by BCH clinical research innovation group. The device is currently being used and piloted in several clinical and research capacities, including a multicentre NIH research consortium, the NHLBI PreCISE network (NCT04129931; https:// preciseasthma. org/). Furthermore, airway oscillometry allows adaptation and distancing options to collect this information at a six foot distance from the subject. Moreover, this manoeuvre does not require a forced exhalation, is less likely to induce coughing and therefore unlikely to generate an aerosol of secretions.

The motivation for conducting this research protocol was highly influenced by the investigators' clinical practice evaluating and managing the respiratory health of children with BPD and recognising the frustrating lack of evidence basis for environmental assessment and intervention in this high-risk group of children. This protocol did not include explicit patient or public involvement in the initial design of the study protocol; however, the recruitment methodology was designed to honour and Open access respect the personal nature of protected health information and patient and family preferences for being contacted to learn about research opportunities. Methodological adjustments to the protocol in response to the SARS-CoV-2 pandemic were made to ensure the health and safety of participants in line with public health recommendations from local and national governing bodies. The findings of this research will be presented in academic settings, including conferences and peerreviewed journals. A small group of participants will be invited to assist the research team in producing a patientlevel mode of dissemination of the main study results to inform participants and linked communities after publication of the primary manuscript.

The study was approved by the Boston Children's Hospital Committee on Clinical Investigation IRB # P00029918, and informed consent and assent were obtained prior to performing any study procedures. The team will disseminate results to the scientific and lay community interested in indoor air quality or BPD in order to avoid unintentional duplication of research. Collaboration will be fostered with investigators who could make use of the collected data and protocols to further scientific discovery in this field.

All results derived from the proposed research will be shared through presentation and discussion at national and international research conferences, as well as through publication in peer-reviewed journals. Results will be presented at national and international scientific meetings focused on organisations interested in environmental health or paediatric respiratory health research. It is also anticipated that at least one to two major publications reporting the primary objectives of this research project and several secondary analysis publications will be generated. Additionally, according to the NIH Policy on Enhancing Public Access to Archived Publications Resulting from NIH Funded Research, all final manuscripts on acceptance for publication will be submitted to the NIH National Library of Medicine PubMed Central (PMC) database, making these openly accessible to the scientific and lay community. Data collected during the course of the proposed study will be made available to the scientific public on submission of a written formal request for data to the principal investigator and coinvestigators of this study. Following the publication of the main study findings, a patient-level communication will describe the key results and be available to participants and linked communities.

The AERO-BPD study was designed to provide a solid foundation for further investigation of environmental exposures and long-term respiratory morbidity in BPD-a novel area of investigation. As the population of children surviving preterm birth increases due to improved neonatal practices, the population of children at risk for long-term respiratory morbidity and poor lung function, so too, will increase. This has enormous implications on the surviving children, families and medical providers charged with caring for these patients, and the healthcare system, as a whole, as this growing segment of the population demands greater resource expenditure over their lifetime. The AERO-BPD study was designed to identify specific harmful components of the indoor environment associated with increased respiratory morbidity in children with BPD and to understand how indoor pollutants will negatively affect lung function in children with BPD, independent of other known epidemiologic risk factors.

School-age children with BPD suffer substantial respiratory morbidity-both as symptoms and lung function impairment. Yet, there is a current lack of evidence basis for factors contributing to this morbidity beyond the NICU experience. Identifying modifiable risk factors, such as poor indoor air quality, and designing interventions to mitigate their effects are of utmost importance to all stakeholders. The impact of these findings will be to identify modifiable risk factors affecting respiratory morbidity in this growing high-risk group of children. These data will be the platform from which targeted interventions can be tested to improve long-term morbidity and will provide greatly needed evidence basis for practicing clinicians, patients and policymakers. Findings from this research will improve our understanding of the environmental influence on children with BPD and offer results from which intervention trials may be designed and a foundation for evidence-based clinical recommendations. In addition to further studies on environmental exposure, this study will provide a foundation for future specific intervention studies to test mitigation strategies for indoor pollutants to improve respiratory health in this population. Furthermore, findings from this study will provide an evidence basis for recommendations related to respiratory health in BPD that currently does not exist and inform guideline creation and public policy.

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Conducting clinical research during the COVID-19 pandemic: protecting scientific integrity

Common household activities are associated with elevated particulate matter concentrations in bedrooms of inner-city Baltimore pre-school children

Development and validation of the Composite Asthma Severity Index--an outcome measure for use in children and adolescents

Sleep environments of children in an urban U.S. setting exposed to interpersonal violence

The relationship between social fragmentation and sleep among adolescents living in

Results of an inner-city schoolbased asthma and allergy screening pilot study: a combined approach using written questionnaires and step testing

International study of asthma and allergies in childhood (Isaac) written questionnaire: validation of the asthma component among Brazilian children

Pediatric sleep questionnaire (PSQ): validity and reliability of scales for sleep-disordered breathing, snoring, sleepiness, and behavioral problems

Development and crosssectional validation of the childhood asthma control test

International study of asthma and allergies in childhood (Isaac): rationale and methods

Validation of a rhinitis symptom questionnaire (Isaac core questions) in a population of Swiss school children visiting the school health services

Management of asthma based on exhaled nitric oxide in addition to guidelinebased treatment for inner-city adolescents and young adults: a randomised controlled trial

PROMIS Pediatric Scale v1.0 -Global Health-7

PROMIS Pediatric -Profile-25 v2.0: HealthMeasures. net

An ultra-brief screening scale for anxiety and depression: the PHQ-4