key: cord-0953155-grml98oa
authors: Walmsley, Amanda M; Arntzen, Charles J
title: Plant cell factories and mucosal vaccines
date: 2003-02-25
journal: Curr Opin Biotechnol
DOI: 10.1016/s0958-1669(03)00026-0
sha: 8efed0e2f2116f528ef08e76585ba0fc56cb261f
doc_id: 953155
cord_uid: grml98oa

Many advances continue to be made in the field of plant-derived vaccines. Plants have been shown capable of expressing a multicomponent vaccine that when orally delivered induces a T-helper cell subset 1 response and enables passive immunization. Furthermore, a plant-derived vaccine has been shown to protect against challenge in the target host. Increased antigen expression levels (up to 4.1% total soluble protein) have been obtained through transformation of the chloroplast genome. In view of these findings, plant-derived vaccines have been proved as valuable commodities to the world’s health system; however, before their application, studies need to focus on optimization of immunization strategies and to investigate antigen stability.

Amanda M Walmsley à and Charles J Arntzen y Many advances continue to be made in the field of plant-derived vaccines. Plants have been shown capable of expressing a multicomponent vaccine that when orally delivered induces a T-helper cell subset 1 response and enables passive immunization. Furthermore, a plant-derived vaccine has been shown to protect against challenge in the target host. Increased antigen expression levels (up to 4.1% total soluble protein) have been obtained through transformation of the chloroplast genome. In view of these findings, plant-derived vaccines have been proved as valuable commodities to the world's health system; however, before their application, studies need to focus on optimization of immunization strategies and to investigate antigen stability.

Emerging and re-emerging diseases, the increasing age and size of the world's population and the threat of biological warfare are challenging the research, development and production arenas of the pharmaceutical industry. There are many effective avenues for the production of recombinant pharmaceutical proteins; however, the main challenge lies in achieving cost-effective production on a large scale. Over the past five years the potential for using plants as bioreactors has become well established. Plant systems offer several advantages: they are economical, using low-cost inputs such as light, water and miner-als; they are easily adaptable to large-scale operations (by growing more plants); they possess minimal risk of contamination with potential human pathogens; the products may not require purification and pharmaceuticals produced this way can be given mucosally, hence simplifying delivery and decreasing overall cost.

Plant-derived pharmaceuticals can be categorized into three areas: antibodies, biopharmaceuticals, and vaccines. Plant-derived antibodies, also referred to as plantibodies, have extensive uses in many areas including bioremediation, disease resistance in plants and industrial purification processes. The most advanced application of plantibodies, however, is the production of antibodies for medical use. Stoger and colleagues [1] provided a recent review on plant-derived antibodies.

There have been many reports of biopharmaceutical expression in plants and these proteins have a diverse range of applications [2-9] (see Table 1 ). In general, however, expression levels of these plant-derived biopharmaceuticals need to be increased before commercial production can be accomplished [10] .

In the past 12 years, substantial research has shown that plant-derived vaccines are feasible commodities. Walmsley and Arntzen give a general description of this technology and its early development [11] . This review highlights the advances made in the past three years and the future directions for the development of plantderived vaccines.

Subunit vaccines comprise specific macromolecules that induce a protective immune response against a pathogen. The use of plants to express antigens for use as vaccines has seen continued interest over the past few years, as witnessed by an increasing number of reports of transgenic plant expression of new antigens and the altered expression of previously reported antigens. A range of different plant and vector systems for the expression of antigens have been investigated and some are summarized in Table 2 [12] [13] [14] [15] [16] [17] [18] [19] [20] . Seed-specific production of the B subunit of heat-labile toxin (LTB) by Streatfield et al. [19] achieved LTB expression levels reaching 1.8% total soluble protein (TSP) and two separate maize breeding programs have increased antigen production by fivefold [19] and tenfold [20] . The investigations of Chikwamba et al. [20] regarding expression of LTB in maize are also the first to report the use of particle bombardment for the production of plant-derived vaccines. Immunogenicity was demonstrated for hepatitis B surface antigen (HBsAg) [12, 21] , the S protein of transmissible gastroenteritis coronavirus (TGEV) [13, 14] and a human immunodeficiency virus 1 (HIV-1) epitope [16] , while successful challenge trials resulted after immunization with P. aeruginosa epitopes [15] , the FP1 epitope of foot and mouth disease virus (FMDV) [17] and LTB [20] .

Additional antigens expressed in plants include the respiratory syncytial virus (RSV) G and F proteins [22, 23] , the VP6 protein of rotavirus [24] [25] [26] , the measles virus (MV) hemagglutinin (H) protein [27] , and an epitope from the major surface antigen of Plasmodium falciparum (PfMSP1) [28] . Although the immunogenicity of the plant-derived RSV G and F proteins and the measles virus H protein were demonstrated in animal trials, the immunogenicity of the VP6 antigen and the PfMSP1 epitope were not tested.

The second report of clinical trials carried out with a plant-derived vaccine appeared in 2000 [29] . Transgenic potatoes expressing the Norwalk virus capsid protein (NVCP) under control of the tuber-specific patatin promoter were orally delivered to human volunteers in phase I/II clinical trials. Of the total number of volunteers who ingested the transgenic potatoes, 95% developed significant increases in specific IgA antibody-secreting cells while 20% developed specific serum IgG and 30% developed specific stool IgA. Although the increase in specific serum antibodies was modest, the use of adjuvants, improved assembly of NV-like particles and increased dosage of the recombinant protein may improve the NVCP immunogenicity (see Update).

Transformation of the plastid genome offers several advantages for antigen expression, including high-level foreign protein expression, removal of the threat of gene silencing, and transgene containment owing to lack of pollen transmission. Daniell et al. [30 ] published the first report of a plant-derived vaccine developed through chloroplast transformation. Integration of an unmodified cholera toxin B subunit (CTB)-coding region into the chloroplast genome resulted in the accumulation of up to 4.1% TSP in tobacco leaves. GM 1 ganglioside-dependent binding assays showed the chloroplast-synthesized CTB to retain its ability to bind to the intestinal membrane receptor; however, immunogenicity studies were not performed.

In attempts to facilitate the harvesting of proteins, antigens have been expressed in a tissue-specific manner in maize kernels [19] , tomato fruit [23] and seeds of transgenic tobacco [31] . Targeting of the major glycoprotein (gB) of human cytomegalovirus (HCMV) to transgenic tobacco seeds was previously described [32] , and further investigations showed that recombinant gB was almost exclusively deposited in protein storage vesicles in mature tobacco seeds [31] .

Owing to their minimalistic nature, subunit vaccines are often difficult to detect in a recombinant protein expression system and/or ineffective at inducing the mucosal immune system. Additional proteins are therefore often Table 1 Applications of biopharmaceuticals produced in plants. [19, 20] employed to either improve the detection or immunogenicity of a subunit vaccine. Both Gil et al. [33] and Dus Santos et al. [34] fused antigens to the gene encoding b-glucuronidase (GUS) to increase ease of detection within the plant expression system. In both instances, transformants were selected on the basis of GUS activity. The GUS fusion to both the 2L21 protective epitope from canine parvovirus [33] and protective epitope from FMDV [34] proved immunogenic. Additionally, mice immunized with the GUS-FMDV epitope fusion were completely protected against challenge with the native virus.

Cholera toxin (CT) has been used effectively as a targeting protein within plant-derived vaccines. Nemchinov et al. [35] described tobacco mosaic virus (TMV) expression of a Vibrio cholerae CTB fusion to an epitope (HVR1) from the hepatitis C virus (HCV). Tobacco plants inoculated with the recombinant TMV produced the HVR1 epitope fused to a functionally active, pentameric CTB. The plant-derived CTB-HVR1 reacted with HVR1-specific monoclonal antibodies and sera from individuals infected with virus from four of the major genotypes of HCV. Intranasal immunization of mice with a crude plant extract containing the recombinant CTB-HVR1 elicited both anti-CTB serum antibody and anti-HVR1 serum antibody that specifically bound to HCV-like particles.

An epitope of the rotavirus enterotoxin protein (NSP4) has also been fused to CTB and expressed in potatoes [36] . Expressing a multicomponent CT fusion vaccine in potato expanded this work. The vaccine consisted of the CTB-NSP4 epitope fusion and an ETEC fimbrial antigen fusion to the component of CTA that associates the A subunit to the CTB subunit (CTA2) [37 ] . The two fusion proteins assembled into cholera holotoxin-like structures that retained enterocyte-binding affinity.

Orally immunized mice generated detectable levels of serum and mucosal antibodies specific for the native antigen. Elevated levels of interleukin-2 (IL-2) and interferon-g (IFN-g) were detected in immunogen-challenged spleen cells from the immunized mice. This indicated the presence of a strong T-helper cell subset 1 (TH1) immune response to the three plant-synthesized antigens. This result was supported by the demonstration of a significant increase in CD4þ lymphocyte numbers. Diarrheal symptoms were reduced in severity and duration in passively immunized mouse neonates following rotavirus challenge. This paper represents the first report of a multicomponent, plant-derived vaccine. This work also demonstrates for the first time passive immunization through a plant-derived vaccine and the induction of a TH1 immune response by an orally delivered, plantderived vaccine.

The vaccination strategy and schedule can have a significant effect on the immune response developed.

Lauterslager et al. [38] investigated the efficacy of a potato-derived LTB vaccine in mice. It was determined that oral administration of plant-derived LTB elicits a systemic and local IgA response in parentally primed, but not naïve, animals. This was in contrast to the results reported by Haq et al. [39] and Mason et al. [40] who reported significant specific antibody responses in naïve animals and showed protection from challenge [40] . The authors' explanations included an inadequate immunization schedule, low antigen dose, low antigen immunogenicity, inability to accurately detect antibodies and interference of tuber material in assays. As tuber materials did not interfere in previous studies performed with the same potato variety [39, 40] , and unsuccessful challenge trials indicated either low or non-existent antibody titers, this investigation highlights the importance of immunization regime and antigen dosage.

After establishing the immunogenicity of plant-derived MV H protein in mice trials [27] , Webster et al.

[41 ] optimized the dose of plant material required to obtain high titer, MV-specific, neutralizing antibodies and examined the boosting of MV H DNA immunization with the plant-derived vaccine. A single-dose DNA inoculation followed by multiple, orally delivered, plant-derived boosters, induced significantly greater quantities of MV-neutralizing antibodies than immunization with DNA or plant-derived vaccine alone. This paper reports the first demonstration of an enhanced immune response to a prime-boost vaccination strategy combining a DNA vaccine with orally delivered plant-derived vaccines.

Reliable methods are needed to quantify plant-derived antigens and ensure their stability. Lee et al.

[42] performed preliminary stability studies. Clover plants expressing the Mannheimia haemolytica A1 leukotoxin 50 fusion protein were harvested and allowed to dry at room temperature and ambient humidity for one to four days. After three days, the clover tissue retained approximately 20% of its initial fresh weight; however, no significant degradation of the fusion protein was observed. Hence, the fusion protein did not require refrigeration for stability. The clover-expressed fusion protein induced an immune response in injected rabbits that recognized and neutralized the native antigen in modified neutral red cytotoxicity assays.

Smith et al.

[43 ] performed a more comprehensive stability study. The quantification of antigenically reactive HBsAg was found to be strongly dependent on the ratio of detergent:cell concentration. A 1-20% w/v sodium ascorbate concentration in the extraction buffer improved the measured levels of monoclonal-reactive antigen 4-to 12-fold. Detergent also influenced antigen stability in cell lysates stored at 48C. Under optimum conditions stability was maintained for at least one month, whereas excess detergent rendered the antigen susceptible to proteolytic degradation. Proteolysis was counteracted by the addition of skimmed milk or its protein component; this stabilized the antigen for up to two months. Also, by altering the sodium ascorbate concentration or buffer pH, the proportion of HBsAg displaying the monoclonalreactive epitopes increased between 8-and 20-fold.

Although antigen stability will have to be investigated on a case-by-case basis, it is obvious that simple in vitro manipulations may prove valuable in increasing the immunogenicity and stability of plant-derived antigens.

Castañ ó n et al.

[44] investigated minimal processing of a potato-derived rabbit hemorrhagic disease virus (RHDV) vaccine consisting of the VP60 gene. Harvested potatoes were peeled, cut into pieces, lyophilized, powdered, stored and used in trials within three months of collection. Rabbits were primed subcutaneously and boosted intramuscularly with extracts made from the potato powder. The rabbits immunized with the transgenic potato elicited specific antibody responses and were protected against challenge with virulent RDHV.

Evidence for plant expression systems leading to the improved manufacture and delivery of vaccines is in hand. Studies have proven plants capable of expression of many different antigens, all of which have demonstrated immunogenicity when tested and some have been shown to provide protection in model and target animals. It has often been stated that there is a need for increased antigen expression levels in plants; however, targeted expression and plant breeding have alleviated this problem. Preliminary investigations into antigen stability, processing and optimal immunization strategies have been performed. However, there is a need for further in-depth studies of individual antigens before plantderived vaccines can be used as a commodity. Recently, concerns have been raised regarding the regulation of plant-derived vaccines and the safety of the food chain from contamination with these pharmaceuticals. For this reason the initial concept of edible, food-delivered vaccines needs to be developed into inexpensive, plantderived, mucosal vaccines that are regulated through prescription and not released like other transgenic plants used for modern agricultural products. Within the United States, two entities currently regulate plant-derived pharmaceuticals, the US Department of Agriculture (USDA) and the Food and Drug Administration (FDA). The USDA currently regulates the location and containment of transgenic plants expressing pharmaceuticals, whereas the FDA ensures quality assurance and quality control. The FDA guarantees that quality assurance and quality control standards are maintained during production and preparation of the materials to ensure safety and efficacy of the materials before human administration. The FDA also determines the relative risk of progressing plantderived pharmaceuticals to licensure through phased clinical trial monitoring. Recently, the FDA released the first draft regulations for the production of plantderived pharmaceuticals [45 ] . The regulatory environment in the US is evolving to encourage the safe yet rapid commercialization of plant-derived pharmaceuticals. The recent developments in plant-derived vaccine production reported here bring this technology ever closer to graduating from the research and development phase. The coevolution of plant-derived vaccine technology and production regulations is ensuring these prototype vaccines are close to becoming useful commodities.

An additional human clinical trial with a plant-derived vaccine was reported in August of 2002 [46] . Transgenic spinach expressing epitopes from the rabies virus glycoprotein (G protein) and nucleoprotein (N protein) fused to the coat protein (CP) of alfalfa mosaic virus (AlMV) was orally delivered to 14 human volunteers. Five of the volunteers had been previously immunized with the conventional rabies vaccine. Three of the five volunteers who had previously been immunized with the conventional vaccine and five of the nine initially naive individuals showed significant specific antibody responses to the rabies virus while none of the control volunteer samples showed significant elevation in rabies-specific antibodies. Seven days after completing the feeding regime, the nine initially naive volunteers were given a dose of the conventional rabies virus vaccine. Three of these individuals showed rabies virus neutralizing antibodies, but none of the five controls showed these antibodies. Although no neutralizing antibody was detected before immunization with the commercial vaccine, there was a clear indication of the potential of the plant-derived rabies vaccine to act as a supplementary oral booster for rabies vaccinations. 

Expression of full-length bioactive antimicrobial human lactoferrin in potato plants

Transgenic plants as factories for biopharmaceuticals

Production of recombinant proteins in transgenic plants: practical considerations

Expression of recombinant human acetylcholinesterase in transgenic tomato plants

Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants

Plants for delivery of edible vaccines

Production of hepatitis B surface antigen in transgenic plants for oral immunization

Oral immunogenicity of the plant-derived spike protein from swine-transmissible gastroenteritis coronavirus

Immunogenicity of porcine transmissible gastroenteritis virus spike protein expressed in plants

Chimeric animal and plant viruses expressing epitopes of outer membrane protein F as a combined vaccine against Pseudomonas aeruginosa lung infection

Chimeric plant virus particles as immunogens for inducing murine and human immune responses against human immunodeficiency virus type 1

Induction of a virusspecific antibody response to foot and mouth disease virus using the structural protein VP1 expressed in transgenic potato plants

Expression of cholera toxin B subunit in transgenic tomato plants

Development of an edible subunit vaccine in corn against enterotoxigenic strains of Escherichia coli

A functional antigen in a practical crop: LT-Bproducing maize protects mice against Escherichia coli heat labile enterotoxin (LT) and cholera toxin (CT)

Oral immunization with hepatitis B surface antigen expressed in transgenic plants

Human respiratory syncytial virus vaccine antigen produced in plants

Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response

Production of recombinant rotavirus VP6 from a suspension culture of transgenic tomato

Rotavirus VP6 expressed by PVX vectors in Nicotiana benthamiana coats PVX rods and also assembles into virus-like particles

Improved production of recombinant rotavirus VP6 in sodium butyratesupplemented suspension cultures of transgenic tomato

Plantderived measles virus hemagglutinin protein induces neutralizing antibodies in mice

Expression of Plasmodium falciparum C-terminal region of merozoite surface protein (PfMSP1 19 ), a potential malaria vaccine candidate, in tobacco

Human immune responses to a novel norwalk virus vaccine delivered in transgenic potatoes

Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chloroplasts

The native CTB gene was transformed into tobacco chloroplasts and resulted in CTB expression levels of up to 4.1% TSP. This is the first report of transgenic chloroplasts manufacturing a plant-derived vaccine

Sorting of glycoprotein B from human cytomegalovirus to protein storage vesicles in seeds of transgenic tobacco

Development of biopharmaceuticals in plant expression systems: cloning, expression and immunological reactivity of human cytomegalovirus glycoprotein B (UL55) in seeds of transgenic tobacco

High-yield expression of a viral peptide vaccine in transgenic plants

A novel methodology to develop a foot and mouth disease virus (FMDV) peptide-based vaccine in transgenic plants

Development of a plant-derived subunit vaccine candidate against hepatitis C virus

Synthesis of a cholera toxin B subunit-rotavirus NSP4 fusion protein in potato

A plant-based multicomponent vaccine protects mice from enteric diseases

This is the first report of plants expressing a multicomponent vaccine, a plant-derived vaccine inducing a TH1 response and passive immunization resulting from a plant-derived vaccine

Oral immunisation of naive and primed animals with transgenic potato tubers expressing LT-B. Vaccine

Oral immunization with a recombinant bacterial antigen produced in transgenic plants

Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): potatoes expressing a synthetic LT-B gene