key: cord-0952296-w6a4lg9n authors: Walker, David H. title: Value of Autopsy Emphasized in the Case Report of a Single Patient with Middle East Respiratory Syndrome date: 2015-12-24 journal: Am J Pathol DOI: 10.1016/j.ajpath.2015.11.001 sha: e6d06cbbbea2ebf9157b23e2ec6c084daedc01e2 doc_id: 952296 cord_uid: w6a4lg9n This commentary highlights the article by Ng et al, emphasizing the need for autopsies to better model infectious diseases such as Middle East respiratory syndrome. A single research-oriented autopsy of a patient with Middle East respiratory syndrome (MERS) defined the pathologic basis of this disease, which has affected approximately 1500 individuals and caused >500 deaths. The pathogenesis of MERS is partially revealed in this investigation by identification of the target organ, lung, and the target cells, type 2 pneumocytes and bronchial submucosal glands, the likely source of virus that results in person-to-person transmission. The study also strongly supports dipeptidyl peptidase 4 (DPP4) as the host receptor for the virus. The truth-testing of this human autopsy highlights the valid and discordant characteristics of animal models in nonhuman primates, transgenic mice expressing the host receptor for MERScoronavirus (CoV), and rabbits. The long interval between the emergence of this dangerous disease 3 years ago and the first autopsy reminds us of the lost opportunity that the decline of the performance of autopsies, particularly research-oriented postmortem examinations in the United States, represents. The article by Ng et al 1 in this issue of the American Journal of Pathology exemplifies the value of a well-performed study of an autopsy. Despite nearly 1500 cases of MERS and >500 deaths, this is the only report of an autopsy. MERS is caused by MERS-CoV that is reminiscent of the severe acute respiratory syndrome-CoV that appeared in China after spreading from an origin in bats by undetermined means into live animal markets where civet cats were an amplifying host, leading to human infections and personto-person spread globally. After >8000 cases with a 10% case fatality rate, severe acute respiratory syndrome was controlled and has not reappeared. In contrast, MERS appeared in the Middle East in 2012 with most cases occurring in Saudi Arabia and the United Arab Emirates and continues to reemerge from its amplifying host, camels, with both camel-to-human and nosocomial and household contact human-to-human transmission occurring. 2,3 MERS has spread to at least 24 other countries, including the United States and South Korea, where >180 cases arose from a single infected individual who had returned from travel to the Middle East. 4 Although MERS occurs in only 4% of household contacts, nosocomial outbreaks occur particularly when infection control measures are deficient. Thus, although the average number of cases generated from one infected individual with MERS is <0.7, indicating that person-to-person spread dies out, the occurrence of new cases of camel-tohuman transmission continues. Although the case fatality rate of laboratory-confirmed MERS is 35%, there are many asymptomatic and mild infections in contacts. In fact, MERS is diagnosed most often in elderly men and individuals with comorbidities, including type 2 diabetes mellitus, chronic renal failure, chronic cardiac disease, hypertension, chronic pulmonary disease, and obesity, and individuals who are immunocompromised. The clinical manifestations of MERS are principally respiratory. The symptoms are nonspecific, including fever, chills, nonproductive cough, and dyspnea. Clinical worsening over a few days leads to respiratory failure with diffuse rales and radiographic abnormalities similar to other viral pneumonias and acute respiratory distress syndrome. Patients also manifest tachypnea, tachycardia, and hypotension. Patients often develop acute renal failure and gastrointestinal symptoms, including nausea, vomiting, and/or abdominal pain. The main mode of transmission remains unknown, although it appears to be via large droplets of respiratory secretions and direct contact, although airborne transmission may also play a role. The value of a well-designed and performed single autopsy study is well illustrated in this report of a research-oriented autopsy that was performed under less than ideal conditions as revealed by the lack of a means for weighing the organs and the 10-day postmortem interval before performance of the autopsy. 1 The article by Ng et al 1 revealed the nature of the disease through the pathologic lesions and supplied critical knowledge for understanding the pathogenesis by identifying the target organ and target cells by immunohistochemistry and visualization of the CoV in lung lesions by electron microscopy. 1 The pathology in this fatal case of MERS included exudative diffuse alveolar damage with hyaline membranes, pulmonary edema, type 2 pneumocyte hyperplasia, interstitial pneumonia that was predominantly lymphocytic, and multinucleate syncytial cells. These lesions comprise the concrete pathologic basis for the patient's respiratory failure and radiologic abnormalities. Bronchial submucosal gland necrosis was also observed. Note that neither acute renal lesions nor central nervous system lesions were identified. The target organ in this patient, the lungs, and the target cells of infection were identified by immunohistochemical detection of MERS-CoV antigens in pneumocytes, multinucleate epithelial syncytial cells, and bronchial submucosal glands. Infection of bronchial submucosal glands is a likely source of viral shedding in respiratory secretions, leading to human-to-human transmission. MERS-CoV antigens colocalized with surfactant-antigen containing type 2 pneumocytes and syncytial cells but not in pulmonary macrophages. DPP4, the receptor for MERS-CoV spike protein S1 subunit, colocalized with MERS-CoV antigen-containing pneumocytes and syncytial cells. This viral tropism is the pathogenic basis for the respiratory manifestations of MERS. No MERS-CoV antigen was detected in kidney, small intestine, colon, liver, spleen, or lymph nodes. The data from this study are discordant with some hypotheses that have been suggested for MERS. No MERS-CoV infection was detected in the kidney, and no acute renal lesions were present, which contrasts with the rare detection of viral RNA in urine of patients. 5 Acute renal failure is a frequent manifestation of severe cases of MERS as occurred in this patient, but the pathophysiologic explanation would appear more likely to be the effects of hypotension on renal function rather than direct viral infection of the kidney, at least in this case. Similarly, the absence of infection in the intestines contrasts with the rare detection of viral RNA in stool and suggests that nausea, vomiting, and abdominal pain, which occur not infrequently in patients with MERS, are unlikely to be caused by viral infection of the gastrointestinal tract. 5 Many in vitro studies of MERS-CoV infection of various cell types were performed and interpreted to suggest viral tropism for various organs for which the autopsy study provided no support. For example, in vitro infection of dendritic cells and macrophages has resulted in dysregulation in the production of cytokines and antigen presentation, and infection of T lymphocytes induced apoptosis. 6 The observations reported in the spleen and lymph nodes of this case do not support the relevance of these studies in human infection. Of course, these conclusions could change with further studies of larger series of cases of MERS. It is ironic that animal models of MERS were developed in nonhuman primates, mice, and rabbits without knowledge of the viral pathogenesis and pathologic lesions of MERS-CoVeinfected humans. An appropriate animal model of an infectious disease should represent the clinical course, organ tropism of the agent, cellular tropism of intracellular agents such as viruses, and pathologic lesions of the human disease after inoculation of an appropriate dose of the agent by the appropriate route that represents the most likely mode of transmission and portal of entry of the agent. There should be an incubation period and duration of illness that agree reasonably with the human course of infection and dosedependent severity, including ideally sublethal and lethal doses. DPP4, the host cell receptor for MERS-CoV, 7 varies among animal species with the receptors of mice, ferrets, and hamsters failing to bind the virus, rendering these species resistant to infection in contrast with the receptors of camels, humans, rhesus monkeys, common marmosets, and rabbits which bind the virus, making the animals susceptible to infection. After combined intranasal, intratracheal, oral, and ocular inoculation or only intratracheal inoculation of an extremely high dose of virus, rhesus macaques develop a mild illness shortly (1 or 2 days) later with viral replication limited to the respiratory tract (nasal mucosa, conjunctiva, and lung), radiographic pneumonia, histopathologic interstitial pneumonia, and viral antigen in type 1 and 2 pneumocytes and alveolar macrophages. 8 This model represents self-limited human MERS as occurs in healthy immunocompetent females and likely differs from most infections in humans by the large dose of inoculum, short incubation period, and infection of alveolar macrophages. Common marmosets inoculated with a large viral dose via combined intranasal, ocular, oral, and intratracheal routes developed moderate-to-severe disease that required euthanasia of two of nine animals on day 4 after a short incubation period with radiographic bilateral pneumonia and viral RNA in nasal and oropharyngeal swabs, blood, respiratory tract, gastrointestinal tract, kidney, heart, adrenal gland, liver, spleen, and brain. 9 Multifocal moderate-tomarked acute interstitial pneumonia on days 3 and 4 was followed by fibrinous consolidation on day 6. Viral antigen was identified in pulmonary lesions, especially type 1 pneumocytes and alveolar macrophages. This model represents severe human disease and pathologic lesions, and it likely differs from human infection in the high dose of inoculum, short incubation period and course, evidence for widely disseminated infection, and infection of predominantly type 1 pneumocytes and alveolar macrophages. A subsequent study of MERS-CoV infection in common marmosets found sublethal mild-to-moderate respiratory disease with no recovery of infectious virus or detection of viral RNA by RT-PCR or viral antigens by immunohistochemistry, modest radiographic evidence of pulmonary disease, and multifocal interstitial pneumonia with few syncytial cells. 10 Both studies found clinical respiratory disease, although differing in severity of illness and virologic results. Development of a nonhuman primate model that is consistently severe will require further investigation and attention to the pulmonary pathology, target cells, and viral dissemination or lack thereof. Because mice are resistant to MERS-CoV, owing to the attachment domain of the viral spike protein not binding to the murine DPP4 receptor, the human DPP4 gene has been expressed in mice. The first successful approach was transduction of mouse lungs by intranasal inoculation of a replication deficient adenovirus vector that express the DPP4 protein. 11 MERS-CoV replicated in the lungs of these mice and was cleared with only mild clinical signs within 6 to 8 days in young mice and 10 to 14 days in aged mice. Subsequently, transgenic mice were generated that express human DPP4 in all tissues. Intranasal inoculation of a high dose of virus in these mice (eventually shown to be 100,000 LD 50 ) resulted in a severe illness with a short incubation period and 100% lethality on day 6. 12 Viral RNA was detected in many organs, and infectious virus was isolated from lungs and brain. Moderate interstitial pneumonia on day 2 progressed with viral antigen detected in type 1 and 2 pneumocytes and microglia, neurons, and astrocytes. A subsequent study of these transgenic mice determined the median infectious dose and LD 50 and investigated the events after intranasal inoculation of MERS-CoV. 13 Five of eight mice inoculated with 10 median tissue culture infectious doses of virus died between days 8 and 13. All mice inoculated with 10 LD 50 of virus died between days 6 and 12. Infectious virus was recovered from the lungs of only two of eight mice and the brain of only one of these mice. None had viral antigen detected in the lung or brain by immunohistochemistry, although viral RNA was uniformly detected in all lung samples and in the brain and gastrointestinal tract samples from days 6 through 10. Pulmonary histopathology progressed from mild multifocal perivascular, peribronchial, and interstitial mononuclear cell infiltrates on day 2 to a peak on day 8. Beginning on day 6 the brain contained pathologic lesions, including perivascular cuffing, apoptotic bodies, microglial activation, and mild meningitis. This transgenic mouse model effectively represents dose-dependent lethal and sublethal MERS-CoV pneumonia with dose-dependent incubation and appropriate pulmonary target cells. Disseminated infection and pathology in the brain were not observed in the human autopsy report; thus, they diverge from the current knowledge of what an ideal model should contain. In addition, infection of the gastrointestinal tract was not observed in the human autopsy. Clearly further autopsy studies are needed to establish firmly the spectrum of human pathology and organ involvement in MERS. Infection of rabbits with a high dose of MERS-CoV resulted in subclinical pulmonary infection without observed histopathologic lesions. 14 Excretion of virus from the upper respiratory tract suggested a potential means of transmission. This model may accurately represent what occurs in asymptomatic human infections. The report by Ng et al 1 emphasizes the importance of defining a novel disease. Until the target organs and the nature of the damage are determined, one does not in reality understand a disease. In the case of MERS development of numerous animal models was undertaken before knowledge of the human pathology. Although these experimental studies were able to suggest the target cells of the virus and histopathology of MERS, only some of the features of the animal models conform to the observations in the human autopsy. Until the truth-testing of a large series of autopsies is reported, judgment will favor those models supported by the observations in a single postmortem examination. Note that the experimental in vitro, in vivo, and structural evidence for DPP4 as a receptor for the MERS-CoV is supported by the autopsy study. Indeed, it is unfortunate that there is inadequate knowledge of the pathology of other emerging infectious diseases. Human monocytic ehrlichiosis, a prevalent life-threatening disease in the United States, is an example. Our own studies of several lethal, sublethal, persistent, and tick-transmission mouse models of ehrlichiosis have determined the pathology and pathogenic and immune mechanisms in mice that are far less understood in the human infections. 15e17 Despite thousands The American Journal of Pathologyajp.amjpathol.org of cases with a 10% case fatality rate in China, our knowledge of the pathology of severe fever and thrombocytopenia syndrome virus infection is limited to a report of a few cases in Japan. 18, 19 We pathologists cannot feel too superior as the decline of autopsies in the United States is dramatic compared with the past. Our potential loss of knowledge includes loss of the opportunity to evaluate the effects of new treatments and the correlations with new imaging tools. An even greater lost opportunity is the inadequate use of autopsies as prospective research tools. The decline in performance of autopsies has resulted in the erosion of expertise of many pathologists whose sporadic assignment to cover an autopsy falls outside their areas of expertise, which is often the surgical pathologic diagnoses of a single organ rather than the comprehensive analysis of the pathophysiologic and morphologic changes in all of the organ systems accrued over a lifetime. The consideration of further reduction in the autopsy requirement for training pathology residents may portend further retreat from the potential gains in knowledge that well-performed postmortem examinations can provide. Because there is only one autopsy report of a novel disease that has killed >500 individuals in the past 3 years should awaken our attention to the value of this final procedure in the search for new medical knowledge. It also supports the careful use of some of the current animal models for development and testing of vaccine candidates and novel therapeutics for the prevention and treatment of the pulmonary disease caused by MERS-CoV. 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