key: cord-0950530-69vlv5ex authors: Zhao, Fengping; Liu, MengYun; Kong, Lingzhen title: Association between red blood cell distribution width‐to‐albumin ratio and diabetic retinopathy date: 2022-03-13 journal: J Clin Lab Anal DOI: 10.1002/jcla.24351 sha: 660379210f19c39b742ec8092fdcd36e8a0d39fa doc_id: 950530 cord_uid: 69vlv5ex BACKGROUND: Diabetes mellitus (DM) has shown a trend of reaching pandemic levels in the world. Chronic inflammation is a key factor in the development of diabetic retinopathy (DR). Red blood cell distribution width‐to‐albumin ratio (RA) is used to assess immune status and the immune response. Our study was conducted to assess the association between DR and RA levels to determine the value of RA in predicting DR. METHODS: The data came from the National Health and Nutrition Examination Survey (NHANES) between 1999 and 2006, The RA was calculated as the Red Blood Cell Distribution Width/Albumin Ratio. Multivariable logistic regression and propensity score‐matched analysis were used to examine the association between RA and DR levels. RESULTS: The clinical and demographic features of the 1,751 patients with DM. The eligible participants included 874 females and 870 males with mean age 62.2 ± 14.0 years, and mean RA 3.2 ± 0.5. RA ≥ 2.9659 was a risk factor for DR (OR = 1.66 95% CI: 1.31–2.11, p < 0.0001). After adjusting for age, sex, race, education, marital status, ratio of family income to poverty, body mass index, fasting glucose, hypertension, and coronary heart disease, RA ≥ 2.9659 was an independent risk factor for DR (OR = 1.64, 95% CI: 1.23–2.19, p = 0.0008). The propensity score‐matched analysis also showed that high RA was an independent risk factor for DR. CONCLUSIONS: Our study shows that RA is a risk factor for patients with DR. The findings of this study should be validated the role of RA in DR in diabetic patients. and society. 6, 8, 9 Early detection of predictors of the occurrence of DR allows doctors to identify high-risk patients earlier for early prevention and quality treatment. Chronic inflammation is the non-specific response of body to injury or stress. 10, 11 There is growing evidence that chronic inflammation is a key factor in the development of DR. 12, 13 Pathological manifestations of chronic retinal inflammation, such as increased retinal blood flow, abnormal stasis of white blood cells, infiltration of neutrophils and macrophages, activation of complement and microglia, up-regulation of cytokines, and increased vascular permeability and tissue edema, have been confirmed in animal models as well as in patients with DR during the development of chronic retinal inflammation. 14 Red blood cell distribution width (RDW) is a parameter that reflects the heterogeneity of red blood cells (RBC) measured using hematology analyzer. 15 The normal range of RDW in human is between 11 and 15%. The larger the RDW value, the greater the difference of red blood cell shape and size. This suggests that there is poor blood and various hematopoietic abnormalities. It was evident that RDW was generally elevated in patients with diabetes and its complications. 16, 17 Moreover, elevated RDW is associated with severe imbalance of RBC homeostasis, abnormal RBC production and survival. This may be associated with oxidative stress, vascular inflammation, and malnutrition such as iron, vitamin B 12, and folic acid deficiencies. In addition, excessive RDW may increase mortality from diabetes mellitus and macrovascular as well as microvascular diseases. Recent studies 18, 19 have shown that albumin is involved in inflammation. The RA is an integrated and novel inflammatory biomarker based on RDW and albumin. Its index was originally used to assess the outcomes in patients with solid stroke 19 and ARDS 20 and is believed to accurately reflect inflammation. However, the role of RA in patients with diabetic retinopathy is still unclear. In the present study, it was hypothesized that patients with DM and higher levels of inflammation were at higher risk of developing retinopathy as measured by RA. Therefore, a cross-sectional study was conducted to assess the association between diabetic retinopathy and RA levels to determine the value of RA in predicting DR. The data came from the National Health and Nutrition Examination Survey (NHANES). NHANES was a 2-year cross-sectoral, hierarchical and multi-stage probability cluster survey. It aimed to gather information on a variety of potential risk factors and nutrition in the non-institutionalized, democratized and U.S. population. The Definition of DM: the selection criteria for diabetes included selfreported diabetes, on anti-diabetes drugs, taking insulin, fasting glucose ≥110 mg/dl, or glycohemoglobin (HbA1c) ≥6.5%. Definition of DR: using the Canon CR6-45NM ophthalmic digital imaging system and Canon EOS 10D digital camera (Canon), or has a doctor ever told that diabetes has affected eyes or that had retinopathy. Demographic characteristics included age, sex, race, marital status, education level, body mass index (BMI), C-reactive protein (CRP), RDW, Albumin, Insulin use, fasting glucose, ratio of family income to poverty (PIR), hypertension, and coronary heart disease. Continuous data were expressed as mean ± standard deviation, and categorical variables were expressed as frequency. Differences in baseline characteristics were compared via an independent sample t test in continuous variables and χ2 tests in categorical variables. For the current study, receiver operating characteristic curve analysis was used to determine the optimal cutoff value for the RA. A multivariable logistic regression analysis was performed to examine the association between RA and diabetic DR thus calculating 95% confidence intervals (CI) and odds ratios (OR). In model 1, there was no adjustment any confounding factors. Age, sex, race, education, marital status, PIR, BMI, fasting glucose chronic conditions including hypertension and CHD was adjusted in model 2. To avoid potential bias, propensity score matching (PSM) was also determined in this study. This study ensured that all reported selection factors for DR were included in the model covariation to further reduce potential confusion. The PSM was performed at a ratio of 1:1, using the 0.05 caliper width of the SD by the propensity score log. After PSM, model 3 was analyzed. All analyses were conducted using R software (version 4.01, http://www.r-proje ct.org). The double-sided significant difference was set at p < 0.05. The clinical and demographic features of the 1,751 patients with DM were as presented in Table 1 . However, it was found that the data of some patients were partly incomplete. The eligible participants included 874 female and 870 males with mean age 62.2 ± 14.0 years, and mean RA 3.2 ± 0.5. The outcomes of this study showed that there was no statistical difference in the age, sex, race, marital status, BMI, and CRP. Patients with DR had high levels of RA, RDW, albumin, fasting glucose insulin using hypertension and CHD (p < 0.05). Smooth curve fitting was used to assess the effects of RA and DR ( Figure 1 ). We constructed various models to assess the independent effects of RA and DR, after adjusting for other potential confounders. Results of logistic regression analysis found that shows effect sizes (OR) and 95% CI ( Note: Data are weighted estimates, and values are presented as means ± standard deviation or means (percentage). Abbreviations: BMI, body mass index; CRP, C-reactive protein; PIR, ratio of family income to poverty; RA, red blood cell distribution width-toalbumin ratio; RDW, red blood cell distribution width. The propensity score-matched analysis was also performed to further verify the relationship between RA and DR. After propensity scorematched analysis, the baseline characteristics of patients in different RA groups were not significantly different ( This is the first study which has shown that RA is an independent risk factor for DR. Further results revealed that patients with DR had higher levels of RA than those without DR. Diabetes is a chronic, low-grade and persistent inflammatory disease. 21, 22 Normal tissue immune cells, such as regulatory T cells, macrophages, and eosinophils, exert anti-inflammatory effects by synthesis of cytokines such as IL-3, IL-10, and IL-4 thereby maintaining microenvironmental homeostasis. 23 Inflammatory activities are inversely associated with albumin synthesis rate which raises blood viscosity, aggregation, and platelet activation. 31 Earlier studies have shown that decreased albumin increases the general population risk for cardiovascular events. In our research, the levels of albumin in patients with higher RDW were considerably lower. HbA1c is produced in red blood cells through a slow and irreversible reaction between hemoglobin and glucose. 32 It reflects the average blood glucose level of body in recent 3 months, with no obvious short-term fluctuation, which is suitable for long-term 33 Abbreviations: CI, confidence interval; OR odds ratio; RA, red blood cell distribution width-to-albumin ratio. a Adjusted for age, sex, race, education, marital status, ratio of family income to poverty, body mass index, fasting glucose, hypertension, and coronary heart disease. red blood cells, such as increased osmotic brittleness of red blood cells, enhanced adhesion, and increased density of red blood cells, that the level of RDW was positively correlated with HbA1c and negatively correlated with blood glucose. 33, 34 Serum albumin is only synthesized in the liver and is a biochemical marker of nutritional status and a major component of colloidal osmotic pressure. Albumin is a negative acute phase protein whose synthesis rate is negatively correlated with inflammatory activity. Currently, there are no effective biomarkers to monitor the DR or to effectively classify patients in order to best assess the efficacy of treatment, and our study has several advantages. The sample size of this study was large enough to determine a significant association between RA and patients with DR. In addition, analysis of detailed covariant data allowed the adjustment for potential confounding factors that might influence the association between RA and DR. However, there are some limitations to the current study. First, cross-sectional study design means causality could not be determined. Prospective studies are thus needed to determine the cause and effect. Second, the data used in this study were extracted from a single blood test. Furthermore, a continuous testing may be more informative than admission testing because of the short life span of the blood cells. Third, RA is easily measured in clinical practice, but the loss of RDW and albumin is common. None. The data that support the findings of this study are available on request from the corresponding author (Lingzhen Kong). 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