key: cord-0950463-d48u5w0h authors: Derraik, José G B; Anderson, William A; Connelly, Elisabeth A; Anderson, Yvonne C title: Rapid evidence summary on SARS-CoV-2 survivorship and disinfection, and a reusable PPE protocol using a double-hit process date: 2020-04-06 journal: nan DOI: 10.1101/2020.04.02.20051409 sha: be4df7a03de718c39fb660583a9be8accc895398 doc_id: 950463 cord_uid: d48u5w0h In the COVID-19 pandemic caused by SARS-CoV-2, hospitals are stretched beyond capacity. There are widespread reports of dwindling supplies of personal protective equipment (PPE), which are paramount to protect frontline medical/nursing staff and to minimize further spread of the virus. We carried out a rapid review to summarize the existing evidence on SARS-CoV-2 survivorship and methods to disinfect PPE gear, particularly N95 filtering facepiece respirators (FFR). In the absence of data on SARS-CoV-2, we focused on the sister virus SARS-CoV-1. We propose a two-step disinfection process, which is conservative in the absence of robust evidence on SARS-CoV-2. This disinfection protocol is based on an initial storage of PPE for ≥4 days, followed by ultraviolet light (UVC), dry heat treatment, or chemical disinfection. Importantly, each of the two steps is based on independent disinfection mechanisms, so that our proposed protocol is a multiplicative system, maximising the efficacy of our disinfection process. This method could be rapidly implemented in other healthcare settings, while testing of each method is undertaken, increasing the frontline supply of PPE, and avoiding many of the upstream issues of supply chain disruption currently being faced. In pandemic situations, such as the current COVID-19 scenario, hospital resources are frequently stretched beyond capacity, as has already occurred in many countries across the globe 1 . Preventing the spread of COVID-19 to and from health care workers and patients relies on the availability and effective use of personal protective equipment (PPE) 1 . PPE includes masks, eye protection, gloves, gowns, and in the event of aerosolgenerating procedures, N95 filtering facepiece respirators (FFR) or FFP2 standard (or equivalent) 2 . Two small studies from the same group in Singapore failed to detect SARS-CoV-2 contamination from PPE, but only on the surface of uncovered shoes 3, 4 . In the case of SARS-CoV-2, it has been estimated that 3.2% of patients in China required intubation 5 . Evidence from the SARS-CoV-1 epidemic show that doctors and nurses involved in the early critical care period and endotracheal intubation of patients were more than 13 times more likely to acquire SARS-CoV-1 infection themselves 6 . Given this results in a significant loss of highly specialised healthcare workers in an already strained workforce, avoidance of cross contamination is critical in all health care settings. The World Health Organization acknowledges the current global stockpile is insufficient, particularly for masks and respirators 2 , and supply of gowns and goggles is also expected to be insufficient. Coordinating the supply chain of PPE in the midst of an epidemic with many closed borders and reduced freight is challenging. Individual behaviour becomes a factor when people are scared or ill-informed 7 , theft of PPE can occur, and local supply chain issues mean that inappropriate use of PPE happens due to lack of supply, despite best-practice guidance on its use 2 . A call for ideas on conserving PPE was made through JAMA on 20 March 2020 8 . One recommendation was reusing PPE. Given the ability of this to rapidly increase supply issues close to the frontline avoiding many of the upstream disruption to the supply chain, this rapid evidence summary was prepared, aiming in particular at the re-utilization of the usually disposable N95 FFRs during the current epidemic. A whole-of-PPE solution has been developed within this protocol, which would be able to be rapidly set up in many healthcare settings. In light of the very recent identification of SARS-CoV-2, there is a near complete lack of data on the survival of this virus in the environment under different conditions, as well as efficacy of disinfection methods. In the absence of data on SARS-CoV-2, we have focused on SARS-CoV-1, which forms a sister clade virus from the same species 9 . • Materials tested included cardboard 10 , wood 11 , plastic [11] [12] [13] [14] , fabric 11, 12 , paper 11, 12 , and metal 10, 11 (Table 1) . • Survival on a range of materials varied somewhat, and even within type (e.g. stainless steel vs copper 10 ). • One study demonstrated survival of 2 days on a disposable polypropylene gown and 24 hours on a cotton gown 12 (Table 1) . • Survival at room temperature and at 40 to 50% relative humidity was as long as 9 days on a polystyrene petri dish 13 and approximately 21 days also on plastic 14 ( Table 1 ). • 9-day survival in respiratory specimens at room temperature 12 , but more than 14 days in dechlorinated tap water at 4°C and >17 days in urine at 20°C 15 (Table 1) . • Of note, a study with a surrogate coronavirus (i.e. transmissible gastroenteritis virus) showed that this virus was detectable on N95 respirators for up to 24 hours 16 . • It is important to highlight the effect of inoculum size on SARS-CoV-1 inactivation, as clearly shown by Lai et al. 2005 12 . While inoculation of a cotton gown at 10 4 TCID 50 /ml led to inactivation in 5 minutes, at 10 6 TCID 50 /ml inactivation took 24 hours. • Only one study found 10 . Virus undetectable after 4 days on plastic and stainless steel, with shorter survival on cardboard and copper 10 (2 days and 4 hours, respectively) ( Table 1 ). • It would be ill-advised to rely on a single study on SARS-CoV-2 to draw any clear conclusions on the virus' survival on different surfaces, especially in light of the existing data on SARS-CoV-1. Thus, until new evidence comes to light, it should be assumed that SARS-CoV-2 may survive for much longer periods under certain conditions, as shown to be the case for SARS-CoV-1. • Unless PPE needs to be immediately re-used, PPE that is visually intact and visually clean could be stored for a stand-down period of at least 4 days before undergoing a given disinfection treatment (i.e. ensuring the virus receives a double-hit). A wide variety of disinfection methods for PPE have been examined and reported in the literature. These can be characterized as either 1) energetic methods (e.g. ultraviolet, dry and wet heat, and microwave generated steam), or 2) chemical methods (e.g. alcohol, ethylene oxide, bleach, and vapourized hydrogen peroxide). Some of these rapidly and significantly affect N95 filter performance (alcohol 17 ), and others require chemical supplies and specialized facilities (e.g. ethylene oxide, vapourized hydrogen peroxide), or are not readily scalable to large numbers of PPE (e.g. microwave generated steam). We focus here on methods that may be easier to implement at a useful scale. Across the ultraviolet (UV) light spectrum, there are three classifications: UVA (320-400 nm), UVB (280-320 nm), and UVC (200-280 nm) 18 . UVC light has much stronger germicidal properties than both UVA and UVB 19, 20 . UVC is strongly absorbed by RNA and DNA bases leading to molecular structural damage via a photodimerization process. This results in virus inactivation, such that they are no longer able to replicate 20, 21 . Thus, the focus of this protocol has been on UVC. • Studies were found only for SARS-CoV-1 (Table 2) . It should be noted that those studies were almost invariably performed on aqueous solutions, in air, or on solid surfaces, i.e. environments that do not directly reflect for example, the micro-environment of N95 FFRs. As a result, the reported applied doses are at best a relative guidance. • Ultraviolet light C (UVC) applied doses varied markedly from 300 to 14,500 mJ/cm 2 , with mixed outcomes (Table 2 ). • At 360 mJ/cm 2 , SARS-CoV-1 had the highest UV D 90 (i.e. required applied dose for 90% inactivation) among nearly 130 viruses from hundreds of published studies summarized by Kowalski 22 . In addition, in protein medium, an applied dose of 14,500 mJ/cm 2 did not completely inactivate the virus 18 (Table 2 ), due to competitive absorption of UV photons by the protein. • UVC is effective against SARS-CoV-1, but efficacy of the applied dose (a function of irradiance and time) appears to be highly dependent on many factors, such as inoculum size, culture medium, and shape and type of material 12, 20, 23, 24 , likely explaining the highly inconsistent findings in the published literature. . CC-BY-NC 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. (which was not peer-reviewed) The copyright holder for this preprint . https://doi.org/10.1101/2020.04.02.20051409 doi: medRxiv preprint • Based on the available evidence it seems that the effect of relative humidity on UVGI efficacy can be considered negligible 22 . • Importantly, the applied dose is not necessarily the same as the actual dose the treated virus receives. While the applied dose is easy to measure experimentally, the received dose is not. If there are shadowing or absorption effects from the surrounding medium, the actual dose reaching the virus will be lower. • The penetration of UV across the multiple layers of an N95 FFR may vary from one model and manufacturer to another 25 . There is some limited evidence that the majority (approximately 90%) of captured aerosols occurs on the outer filter layer on an N95 FFR 26 . Therefore, providing a larger UV dose on the outside surface may be desirable. • Overall, the effective applied dose is unclear, but appears to be high in comparison to other viruses. • Mills et al. (2018) reported on a more extensive set of tests on N95 masks using H1N1 viruses, and included the effects of soiling agents (artificial saliva and/or skin oil) that could reduce the efficacy of UV exposure 27 . Fifteen different N95 models were tested from a variety of manufacturers, and both the facemask and straps were monitored. All FFRs were disinfected to a level of at least 3 log (i.e. 99.9%), even in the presence of soiling agents, when the UV dose was 1,000 mJ/cm 2 . Similarly, Heimbuch & Harnish (2019) showed complete disinfection of SARS-CoV-1 from FFR coupons in the presence of artificial saliva (mucin) and artificial skin oil (sebum) 23 . • We estimate that the minimum applied UVC dose for effective deactivation of SARS-CoV-2 on N95 FFRs would likely be close to 1,000 mJ/cm 2 , particularly in light of the mask's porous surface (as compared to a smooth surface material), as shown by Heimbuch & Harnish's 2019 study 23 . • Note that the studies showing SARS-CoV-1 survival at higher doses were most likely confounded by the aqueous media (often with added protein), which would absorb UVC photons, reducing the actual dose reaching the virus. Heat treatment is one of the most common methods for virus deactivation. Heat induces structural changes in virus proteins, disrupting the specific structures necessary to recognize and bind to host cells 28 . • Studies found only for SARS-CoV-1 ( Table 2 ). It should be noted that it is not easy to extrapolate the results from most heat treatment studies reported here. They were often performed with the virus exposed while in solutions, which are mechanistically different from surface contamination, as one would most likely encounter on PPE that is not heavily soiled, particularly N95 FFRs. • Environments with lower temperatures seem to be more favourable for virus survival and increased transmission rates 14, 29, 30 . While the efficacy of heat treatment appears to be affected by relative humidity 14 , this relationship for SARS-CoV-1 is unclear, as almost all reported experimental studies failed to report on relative humidity (Table 2) . However, the association between temperature and relative humidity was not monotonic for other coronaviruses, with virus survival lowest at moderate relatively humidity (50%) 30 . • Two studies reduced SARS-CoV-1 to below levels of detection with exposure to 56°C at 20 min 18 and 60 min 31 , but one study showed that at 56°C for 30 min heat treatment was ineffective 13 in protein medium ( Table 2 ). • Heat treatment at 60°C for 30 minutes inactivated SARS-CoV-1 in two studies 13, 18 , irrespective of protein concentrations. However, in Duan et al. (2003) 11 the virus was only inactivated at 67°C after 60 minutes, while in Darnell et al. 19 inactivation occurred only after 90 minutes at 65°C ( Table 2 ). • Overall, heat treatment at 60°C for 90 minutes would reduce SARS-CoV-1 to below levels of detection according to all five heat-treatment studies reported in Table 2 . • The applied UVC dose should be at least 1,000 mJ/cm 2 , but we recommend an initial conservative dose of 2,000 mJ/cm 2 [applied to each side of N95 FFRs, i.e. wearer-facing and outer sides] to account for possible errors in applied dose estimation, effects of different materials, the challenge to reach the inner filtering layers of FFRs 25 , as well as the uncertainty regarding the actual susceptibility of SARS-CoV-2 to UVGI. • We also recommend heat treatment at 60°C for 90 minutes to treat PPE. This is most likely a very conservative protocol when applied to surface contamination, but in the absence of more precise data, we recommend erring on the side of caution. The 90minute period of exposure is advisable to ensure there is adequate heat transfer to the inner layers of the FFRs, particularly if a number of masks are being treated at the same time (in which case we would caution against stacking them). • While we cannot recommend a target relative humidity due to the paucity of data for SARS-CoV-1, moderate levels are likely to be more desirable (i.e. 40% to 50%). • We advise against attempts to disinfect and reuse soiled PPE, as studies using both UVGI and heat treatment show a protective effect of protein and aqueous substrata on SARS-CoV-1 survival. • Unpublished experimental data from our group showed that there is minimal UVC radiation on the wearer-facing side of N95 FFRs when the outer side is irradiated (outer 7.34 mW/cm 2 vs inner 0.10 mW/cm 2 ). There are reports of widespread SARS-CoV-2 infection among frontline medical staff 32 , thus, it has to be assumed that SARS-CoV-2 contamination of N95 FFRs would likely occur on both sides, particularly when there . CC-BY-NC 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint . https://doi.org/10.1101/2020.04.02.20051409 doi: medRxiv preprint is strong evidence of asymptomatic transmission 33-35 . Therefore, we strongly recommend that both wearerfacing and outer sides of N95 masks be equally treated at the recommended UVC dose. • Table 3 summarizes six studies that have looked at the effects of UVGI disinfection on the performance and structure of N95 FFRs. • In five studies, applied doses varied somewhat from 180 mJ/cm 2 to 6,900 mJ/cm 2 , but there were no observed effects on the N95 FFRs filter aerosol penetration, filter airflow resistance, fit, odour detection, comfort, donning difficulty, or physical appearance ( Up to 20 UVGI cycles (total applied UVC dose 20,000 mJ/cm 2 ) did not have a meaningful effect on fit, airflow resistance, or particle penetration for any model. Strap strength was unaffected by 10 UVGI cycles (total applied dose 10,000 mJ/cm 2 ), but 20 cycles had some effect on certain models. • Lindsley et al. 36 went further, estimating the cumulative effect of extremely high exposures of N95 FFRs to UVC in order to mimic repeated cycles of UVGI treatment. Their lowest applied dose of 120,000 mJ/cm 2 reduced the bursting strength of the four N95 models tested by 11% to 42% (depending on the model and the individual layer), with very minor effects on filter aerosol penetration and filter airflow resistance (Table 3 ). An applied dose of 590,000 mJ/cm 2 reduced the breaking strength of straps from the four N95 FFR models tested by 10% to 21% 36 . It should be noted that their lowest dose is 120,000 mJ/cm 2 , which is 60 times higher than the conservative minimum dose of 2,000 mJ/cm 2 we recommend for SARS-CoV-2 inactivation. • Table 4 describes five studies that examined the effects of heat treatment on the performance and structure of N95 FFRs. • Two studies looked at dry heat treatment at 80°C 17,37 , reporting no meaningful effects on filter particle penetration and leading to no obvious signs of damage (Table 4 ). • Three studies looked at moist heat incubation at 60°C and 80% relative humidity, two for 15 minutes 38,39 and one for 30 minutes 40 (Table 4 ) -there were no meaningful effects on filter aerosol penetration or filter airflow; most FFRs were undamaged, but in the three studies there was separation of the inner foam nose cushion from the FFR body in one particular model. • While two studies have looked at the effects of 3 heat treatment cycles for 15 minutes 38 and 30 minutes 40 at 60°C on N95 FFRs, no peer-reviewed studies seem to have looked at the potential effects of more than 3 heat treatment cycles or multiple cycles of longer duration on N95 FFRs. The exception is Liao et al. (2020) 41 , who have recently reported that 20 cycles of dry heat at 75°C for 30 minutes did not affect the filtration efficacy of the key fabric in N95 FFRs. However, due to their methodology it was not possible to ascertain whether the fit of the masks for example, would be affected by their treatment protocol. • It is worth noting that N95 FFRs are mostly made of polypropylene 42 , whose maximum operating temperature is approximately 80°C 43 , so that heat treatment approaching this temperature is probably illadvised. • We recommend the use of UVGI at a conservative applied UVC dose 2,000 mJ/cm 2 (for each surface) for N95 FFRs. • Based on the available evidence, there are uncertainties about using our recommended heat treatment at 60°C for 90 minutes for N95 FFRs, as the extended time required for heat treatment may have adverse effects that could compromise its safety for reuse, especially after multiple disinfection cycles. However, a recent report by Liao et al. 41 suggests that our proposed heat treatment regimen could be applied to N95 FFRs, and could therefore be adopted in the absence of UVC treatment. • Apart from N95 FFRs, in a pandemic situation the supply of other PPE will be seriously affected, including isolation gowns, surgical masks, face shields, and goggles. • Heat treatment (at 60°C for 90 minutes) is recommended for isolation gowns (due to their size and folds) and surgical masks (due to their folded construction). • Face shields are made of thin plastic, and usually have a foam-like material on the area that is in direct contact with the face, which would be difficult to clean with chemical disinfectants. As the shields may be damaged at 60°C we therefore recommend that these are treated with UVGI. However, repeated UVGI treatment could affect the clarity of the shields leading to 'fogging', in which case they should be discarded. • Goggles and other eyewear should be immersed for at least 10 minutes in a chlorine solution at a conservative dose of 5,000 mg/l, which would account for the gradual reduction in chlorine concentration throughout the day. Alternatively, these could be cleaned with an alcohol solution at ≥80%, which should be left for at least 30 seconds 44 . Afterwards, the goggles/eyewear should be rinsed thoroughly with warm water to remove the disinfectant solution, which could otherwise damage the equipment or cause skin irritation on the . CC-BY-NC 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint . https://doi.org/10.1101/2020.04.02.20051409 doi: medRxiv preprint wearer. Also, as goggles and other eyewear can be made of different materials, we recommend testing to make sure the disinfectant would not damage them (e.g. 'fogging' the lenses) before implementing a chemical disinfection procedure. • According Fisher & Shaffer 2014 42 extended use is preferable over limited re-use due to a lower risk of contamination with lesser contact with FFR surface. • However, extended used leads to an increase in noncompliant behaviours (e.g. adjusting or touching the N95) over time 47 , increasing the risk of selfcontamination. • 97% of 542 first-line healthcare workers in China during the COVID-19 response had some form of skin damage, which increased with longer wear of N95 FFRs 48 . An accompanying editorial highlighted that this increases the likelihood of non-compliant FFR-wearing behaviour, and consequently an increased risk of viral transmission 49 . • As prolonged skin breakdown increases health care workers susceptibility to infection and improper PPE use, access to virtual dermatology clinics for healthcare workers is strongly recommended to manage and treat skin breakdown in health professionals wearing PPE for extended periods. • Due to the widespread use of alcohol-base disinfectants, it is important to emphasise that masks and respirators should not be sprayed with alcohol. Alcohol can remove the electrostatic charge from the respirator filter material, severely reducing the filter's effectiveness at collecting particles, as shown by a number of studies 17 . Given the dearth of evidence of PPE disinfection in SARS-CoV-2, our recommendations have been conservative, and have concluded a double-hit process would be favourable to one, until robust evidence on the efficacy of individual methods against SARS-CoV-2 is available. Importantly, each of the two steps is based on two different and independent disinfection mechanisms. Therefore, our dual-step disinfection protocol is a multiplicative process, where if each step can achieve a 3-log reduction, the two consecutive steps would theoretically achieve a 6-log reduction in SARS-CoV-2 (i.e. 99.9999%). Given this lack of evidence, clinicians have applied a WIWI (would-I-wear-it) test to the process for developing protocol recommendations. Further, based on the literature that was examined during the preparation of this manuscript, the proposed methodology would most likely achieve disinfection against other pathogenic organisms. As previously mentioned, PPE that are obviously soiled with organic matter should not be reused, as their disinfection is more difficult to achieve using procedures that would not damage them. Based on the available evidence, the following disinfection steps are proposed, as outlined in Figure 1 . At point of doffing PPE, wearer is to remove and inspect items, looking for any damage or soiling (e.g. bloodstains or presence of organic material). If the PPE is damaged or visibly contaminated, this is to be placed in a bin for biohazard waste. If not damaged or contaminated, PPE is to go into a separate clearly marked bin for re-use. This PPE to be bagged and transported in bin to storage area, where the process outlined below will begin. of PPE (including straps); any soiled and damaged PPE to be discarded, intact PPE to be stored. All intact PPE to be stored for at least 4 days in a specially designated area (if conditions can be determined, we suggest ~20 o C and 40-50% relative humidity), ensuring no direct contact between items, and minimizing any creases in material. Locally this will be rooms or enclosures where gowns and other PPE can be hung in four rotating enclosures/rooms, and left for four days. 3a. UVGI -After completion of the mandatory standdown storage period, N95 FFRs and plastic face shields to be treated in the designated UVGI chamber at an applied dose of 2,000 mJ/cm 2 , with this dose applied to each side, i.e. wearer-facing and outer sides. 3b. Heat treatment -After completion of the mandatory stand-down storage period, polypropylene gowns and surgical masks to undergo heat treatment at . CC-BY-NC 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint . https://doi.org/10.1101/2020.04.02.20051409 doi: medRxiv preprint 60°C for 90 minutes, possibly at moderate relative humidity (40-50%). 3c. Chemical disinfection -After completion of the mandatory stand-down storage period, eyewear must be disinfected with the appropriate high-grade agent, either through soaking or use of appropriate wipes. After UVGI, heat treatment, or chemical disinfection, careful reinspection of PPE (including straps) must take place; any PPE with any sign of damage must be discarded; intact PPE to be packaged for re-use, after being appropriately marked as PPE derived from disinfection, including the number of the disinfection cycle. Frontline staff to ensure that any decontaminated PPE fit properly as new; at any sign of suboptimal fit, decontaminated gear to be immediately discarded. Afterwards, a new disinfection cycle to begin. It should be noted that N95 FFRs should probably be discarded after the fifth re-use. An exception to this rule would be under extreme circumstances, where the alternative to further re-use of suboptimal PPE would be not wearing any protection at all. This protocol provides recommendations for a pragmatic disinfection process for all PPE, that could be rapidly implemented, based on best available evidence. A double-hit process has been proposed due to the immediate urgency of the issue in the current pandemic. Testing of this protocol is in planning stages, but its conservative double-hit approach would most likely achieve disinfection. Based on a total estimated 10% loss of N95 FFRs over 5 cycles, this procedure would increase supply by 400%. We are currently finalizing the tests of the prototype of a UV chamber that would be able to treat a batch of N95 FFRs at the required does in less than 4 minutes. Careful design of heat chambers and UVC cabinets for re-use of PPE will not only address the problem of short-term supply in the frontline during the pandemic, but also likely lead to considerable cost-savings in the long term. Further, it would also improve the environmental footprint of a given healthcare facility allowing for long-term reuse of PPE, as according to estimates from US hospitals for example, 5.17 tons of waste are generated per staffed bed every year 50 . It is intended that results of protocol testing will be made available as soon as feasible. It is the right of every healthcare worker responding to the current pandemic to have PPE available not only for their protection, but also to reduce the spread of COVID-19 51 is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint . https://doi.org/10.1101/2020.04.02.20051409 doi: medRxiv preprint . CC-BY-NC 4.0 International license It is made available under a is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity. The copyright holder for this preprint . https://doi.org/10.1101/2020.04.02.20051409 doi: medRxiv preprint • At 590 J/cm 2 the mean strap breaking strengths decreased by 10-21% • The lowest applied dose tested of 120 J/cm 2 reduced the bursting strength of the four models tests by 11% to 42% (depending on layer and model). Sourcing personal protective equipment during the COVID-19 pandemic World Health Organization. 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Coronavirus disease (COVID-19) outbreak: rights,roles and responsibilities of healthworkers, including key considerations for occupational safety and health SARS-CoV, influenza A and syncitial respiratory virus resistance against common disinfectants and ultraviolet irradiation Effectiveness of three decontamination treatments against influenza virus applied to filtering facepiece respirators • This is an unpublished report that has not been peer-reviewed, with poorly described methods.• The ten 30-minute cycles did not affect the fabric's filtration efficiency • However, in the absence of any information on the irradiance, it is not possible to ascertain the actual applied UVC dose. • This is an unpublished report that has not been peer-reviewed, with poorly described methods.• No change in filtration efficiency after 3 cycles • Drop in filtration efficiency (from ~97% to ~85%) after 5 cycles, explained by the authors as due to loss of static charge of the fibers.FFR, filtering facepiece respirators; MHI, moist heat incubation; RH, relative humidity.