key: cord-0942676-j4p4ehex authors: Tiwari, Lokesh; Shekhar, Shashank; Bansal, Anmol; Kumar, Subhash title: COVID-19 associated arterial ischaemic stroke and multisystem inflammatory syndrome in children: a case report date: 2020-10-27 journal: Lancet Child Adolesc Health DOI: 10.1016/s2352-4642(20)30314-x sha: ab57b378354ef8ae016f3b9bb8462ab86206b406 doc_id: 942676 cord_uid: j4p4ehex nan A 9-year-old girl was admitted to the paediatric intensive care unit (PICU) with a history of high-grade fever for 14 days, throbbing frontal headache, vomiting, and progressive weakness on the right side of her body for 5 days. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA was detected on nasopharyngeal swab by RT-PCR on presentation. On admission to the PICU (day 1), she had bilateral non-purulent conjunctivitis, high-grade fever (axillary temperature using digital thermometer of 39·4°C), blood oxygen saturation of 98%, a heart rate of 64 bpm, tachypnoea, and hypertension (132/102 mm Hg). The patient had a Glasgow Coma Score (GCS) of 11 (E3, V2, M6), upper motor neuron type rightsided seventh cranial-nerve palsy, complete hemiplegia, brisk deep tendon reflexes, and extensor plantar response on the right. Her pupils were normal and she had no signs of meningeal irritation. Her paediatric quick sequential organ function assessment (qSOFA) score was 2 (of 3) on admission. 1 The suspected causes for her neurological signs included COVID-19 associated encephalitis or stroke. She received tier 1 and tier 2 management for raised intracranial pressure-ie, mechanical ventilation, thermoregulation, sedation, and head-elevation to 30° (tier 1) and osmotherapy (glycerol, 3% hypertonic saline, and intermittent mannitol) and intermittent hyperventilation (tier 2), and other supportive care (eg, optimisation of intravascular volume, maintenance of normoxemia, and prevention and treatment of fever and seizures). 2 Empirical antibiotics included ceftriaxone, vancomycin, and azithromycin. She had normal blood counts and renal function, mild transaminitis, high CRP, high ESR, high D-dimer, and increased triglyceride and ferritin concentrations, suggestive of a hyper inflamm atory response (table) . Chest x-ray showed bilateral ground-glass opacification and reticulonodular opacity. She deteriorated with worsening GCS, bradycardia, and cardiac arrest on day 3 of PICU admission. Return of spontaneous circulation was achieved after 2 mins of chest compression and ventilation. Her SOFA score worsened from 2 to 12, therefore requiring higher ventilatory, vasoactive, and inotropic support for respiratory failure, shock, and autonomic instability (appendix). Echocardiography did not show valvular defect or coronary artery dilatation. Her ECG, serum sample of myocardial creatine phosphokinase (CPK-MB), and troponin I measurements were normal. An unenhanced CT scan showed multifocal discrete and confluent hypodensities, suggestive of infarcts, in the genu and adjacent body of corpus callosum, left basal ganglia, and bilateral thalami, with mild oedema and mild mass effect over the lateral ventricle. Subsequently, a CT angiography showed multifocal smooth stenosis of both intracranial internal carotid arteries, right middle cerebral artery, and both A2 segments of the anterior cerebral arteries. Diffuse narrowing of the M2 and M3 segment branches of both middle cerebral arteries was also seen (figure). To further investigate rarer causes of childhood stroke the following tests were done, all were negative: anti-nuclear antibody, vasculitis profile, and sickle cell screen (table). There was no history of neck trauma that suggested arterial dissection. She had no history of skin lesions that were suggestive of varicella (chickenpox) in the past or during current illness. Cerebrospinal fluid examination showed pleocytosis with slightly increased protein (table) but tested negative for SARS-CoV-2 using RT-PCR. No organism was detected in cerebrospinal fluid or blood despite extensive tests for possible organisms (table) . Test for IgM and IgG antibody for varicella zoster virus could not be reported because the test kit was not available. Additional treatment included intravenous immunoglobin (2 g/kg over 2 days), methylprednisolone (30 mg/kg per day intravenously for 5 days) followed by dexamethasone (0·15 mg/kg per day for 2 weeks), remdesivir (5·0 mg/kg by intravenous infusion over 1 h, followed by 2·5 mg/kg per day by intravenous infusion for 5 days), and low-molecular-weight heparin (1 mg/kg subcutaneously twice daily for 2 weeks followed by once daily for 1 week). Her clinical status improved progressively (appendix), and she was weaned off mechanical ventilation on day 12. Her serum CRP, ferritin, and lactate dehydro genase values returned to normal. COVID-19 RT-PCR on nasopharyngeal swab and bronchoalveolar lavage was negative on day 12 of stay in the PICU. A serum COVID-19 antibody test (done after 4 weeks of admission) was positive. She showed slow but consistent improvement over the next 3 weeks, reaching a GCS of 13 (E4, V3, M6) and power of 2-3 of 5 on the affected side. She transferred from PICU to a paediatric ward where she continues to receive psychomotor rehabilitation for her residual illness. COVID-19, a global health emergency, caused by the highly pathogenic SARS-CoV-2, has affected more than 28 million people and caused 900 000 deaths, as of Sept 13, 2020. 3, 4 Most of the children infected with SARS-CoV-2 either are asymp tomatic or have upper respiratory tract infection. 5 Paediatric multisystem inflammatory syndrome tempo rally associated with COVID-19 (PIMS-TS) or multi system inflammatory syndrome in children (MIS-C) is an emerging lifethreatening non-respiratory compli cation of COVID-19 that can present at any time during the course of illness, but commonly at 1-6 weeks after infection. 6 See Online for appendix evidence suggests this syndrome is related to uncontrolled inflammatory response and cytokine storm following infection with SARS-CoV-2; however, understanding of the mechanism remains poor. 6 Global and population-specific incidence of MIS-C is unknown. WHO has formulated a case defi nition and global registry for MIS-C. 10 Our case of a 9-year-old girl with fever for 14 days, bilateral non-purulent conjunctivitis, shock, elevated D-dimer, ESR, CRP, sterile blood culture, positive COVID-19 RT-PCR, and no respiratory symptoms, satisfies WHO diagnostic criteria of MIS-C. 10 This patient presented with initial clinical manifestations of acute ischaemic stroke, which has so far not been reported in children. In one of the first reports of PIMS-TS, the patient who died despite extensive organ support (mechanical ventilation, renal replacement therapy, and extracorporeal membrane oxygenation) had developed right middle cerebral artery and ACA ischaemic infarcts. That patient was confirmed as SARS-CoV-2 positive at post-mortem analysis. 11 In our case, early manifestation of stroke and aggressive therapy to halt the cytokine storm seems to be crucial for reaching a positive outcome. The differential diagnosis of stroke in children is broad. viral encephalitis, bacterial meningoencephalitis, brain abscess, tubercular meningitis, and cardiac thromboembolism-were ruled out. There was no history to suggest varicella encephalitis, although antibodies for varicella zoster virus could not be tested. We also ruled out other rare causes of acute ischaemic stroke (table) . Neuro logical presentations associated with COVID-19 and similar strains in adults include altered consciousness, headache, dizziness, anosmia, dysgeusia, seizures, stroke, Guillain-Barré syndrome, and neuropsychiatric symp toms. 7,8 SARS-CoV-2 is reported to have a 7·6 times increase (95% CI 2·3-25·2) in the risk of resulting in a stroke in comparison with other coronavirus and seasonal infections in adults. 12 Patho physiology of acute ischemic stroke in COVID-19 is not fully elucidated yet, although possible mechanisms could be immune mediated or para-infectious events, a hypercoagulable state from systemic inflammation and cytokine storm, viral mimicry of the host resulting in autoantibodies, viral super antigen sequences, antibody or T-cell recognition of viral antigens, or formation of immune complexes. 6, 8, 13, 14 Direct viral-induced endothelitis or endotheliopathy, leading to angiopathic thrombosis is also seen in some neurotropic viruses such as Varicella zoster. 15 Endothelial infection by SARS-CoV-2 in the kidney, heart, bowel, and lung at autopsy is reported but cerebral vessels have not yet been investigated. 16 Initial neuro imaging findings, similar to our described case, are reported in a few adult cases of COVID-19 infection. 7 In our case, RT-PCR for COVID-19 was positive on nasopharyngeal swab but negative in cerebrospinal fluid. Cerebrospinal fluid is often negative for viral genetic material in influenzaassociated encephalopathy. 8, 17 The negative RT-PCR for the cerebro spinal fluid sample could be attributed to low viral load in the sample, transient viraemia, and delay in testing after symptom appearance. 18 MIS-C is rare; of 662 cases reported so far, 19 only the case in this report has presented with acute ischaemic stroke. Consid ering the emerging evidence of this disease, clini cians should include SARS-CoV-2 in their differential diag nosis for children presenting with new neurological symp toms, positive inflammatory markers, and suggestive imag ing findings while explor ing other possible causes. Aggressive therapy to halt the cytokine storm and relevant supportive care while considering differential diagnoses is crucial for reaching positive outcomes in children. Further studies are required to understand the patho genesis of ischaemic stroke and assess the neurological and cognitive outcomes in children with COVID-19. LT made the treatment decisions, supervised the initial draft, reviewed the studies, and did the planning, analysis, and final drafting of the Case Report. SS and AB were involved in patient management, literature search, and writing of the initial draft. SK helped in synthesis and analysis of radiographical information. All the authors contributed to the final manuscript. LT and SS accessed and verified the data. LT had the final responsibility to submit for publication. We declare no competing interests. Abstract PCCLB-42: new predictive score for PICU: pediatric quick SOFA score-AIIMS Patna model Management of intracranial hypertension A novel coronavirus from patients with pneumonia in China COVID-19) outbreak situation SARS-CoV-2 Infection in children COVID-19 and multisystem inflammatory syndrome in children and adolescents Encephalopathy in patients with COVID-19: a review Neurologic and radiographic findings associated with covid-19 infection in children Clinical characteristics of children and young people admitted to hospital with COVID-19 in United Kingdom: prospective multicentre observational cohort study Multisystem inflammatory syndrome in children and adolescents temporally related to COVID-19 Hyperinflammatory shock in children during COVID-19 pandemic Risk of ischemic stroke in patients with COVID-19 versus patients with influenza Neurological associations of COVID-19 Central nervous system involvement by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) Varicella zoster virus vasculopathies: diverse clinical manifestations, laboratory features, pathogenesis, and treatment Endothelial cell infection and endotheliitis in COVID-19 Systematic SARS-CoV-2 screening in cerebrospinal fluid during the COVID-19 pandemic Diagnostic techniques for COVID-19 and new developments Multisystem inflammatory syndrome in children: a systematic review Ethical clearance was granted by the All India Institute of Medical Sciences Patna ethics committee (approval no AIIMS/Pat/IEC/2020/535). Written informed consent for publication was obtained from the patient's parents. We thank Arun Prasad, Pradeep Kumar, Bhabesh Kant Chowdhary, Amit Sinha for contributing to the clinical management of the patient. All authors have access to all the data in the study. LT and SS have accessed and verified all the data in the study.